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Dental pulp stem cells are dental pulp-derived mesenchymal stem cells that originate from the neural crest. They exhibit greater potential for the treatment of nervous system diseases than other types of stem cells because of their neurogenic differentiation capability and their ability to secrete multiple neurotrophic factors. Few studies have reported Alzheimer’s disease treatment using dental pulp stem cells. Rat models of Alzheimer’s disease were established by injecting amyloid-β1–42 into the hippocampus. Fourteen days later, 5 × 106 dental pulp stem cells were injected into the hippocampus. Immunohistochemistry and western blot assays showed that dental pulp stem cell transplantation increased the expression of neuron-related doublecortin, NeuN, and neurofilament 200 in the hippocampus, while the expression of amyloid-β was decreased. Moreover, cognitive and behavioral abilities were improved. These findings indicate that dental pulp stem cell transplantation in rats can improve cognitive function by regulating the secretion of neuron-related proteins, which indicates a potential therapeutic effect for Alzheimer’s disease. This study was approved by the Animal Ethics Committee of Harbin Medical University, China (approval No. KY2017-132) on February 21, 2017.

Dental pulp stem cells are a type of adult stem cells with strong proliferative ability and multi-differentiation potential. There are no studies on treatment of vascular dementia with dental pulp stem cells. In the present study, rat models of vascular dementia were established by two-vessel occlusion, and 30 days later, rats were injected with 2 × 107 dental pulp stem cells via the tail vein. At 70 days after vascular dementia induction, dental pulp stem cells had migrated to the brain tissue of rat vascular dementia models and differentiated into neuron-like cells. At the same time, doublecortin, neurofilament 200, and NeuN mRNA and protein expression levels in the brain tissue were increased, and glial fibrillary acidic protein mRNA and protein expression levels were decreased. Behavioral testing also revealed that dental pulp stem cell transplantation improved the cognitive function of rat vascular dementia models. These findings suggest that dental pulp stem cell transplantation is effective in treating vascular dementia possibly through a paracrine mechanism. The study was approved by the Animal Ethics Committee of Harbin Medical University (approval No. KY2017-132) in 2017.

In this article, seven filter algorithms were compared. The Lee sigma method was more suitable for estimating soil moisture (SM) than the other filtering methods under different land cover types. First, we used a combination of roughness and the dual-polarized Sentinel-1A backscattering coefficients (VV and VH) to estimate SM in bare soil areas. Second, we employed water cloud model (WCM) to remove the influence of vegetation signals on the land surface backscattering and estimate SM in vegetation-covered areas. SM was also retrieved by modified soil moisture monitoring index (MSMMI) and modified perpendicular drought index (MPDI) of Sentinel-2A images. The results show that MSMMI can more accurately monitor SM in bare soil areas, which was slightly better than synthetic aperture radar (SAR) results. The SAR backscattering coefficients after the removal of vegetation influence by WCM can more precisely estimate SM in vegetation-covered areas, which is significantly better than MSMMI and MPDI, especially in high vegetation-covered areas. Optics and SAR differ in their abilities to estimate SM under different land cover, but the powerful fitting ability of machine learning can make full use of their advantages. We employed the generalized regression neural network (GRNN), support vector regression (SVR), random forest regression (RFR), and deep neural network (DNN) algorithms to estimate SM combining Sentinel-1A with Sentinel-2A images. The estimation accuracies of SM by regression algorithms were higher than those by the semiempirical SAR and optical models. The accuracy of estimated SM by DNN was higher than that of GRNN and RFR, which were better than SVR.

This article aims to explore the applicability of SMMI (soil moisture monitoring index), MSMMI (modified soil moisture monitoring index), PDI (perpendicular drought index), and MPDI (modified perpendicular drought index) in estimating soil moisture (SM) in farmland. The random forest classifier was used to obtain two-stage land cover types maps. The sensitivity of Sentinel-2 spectral bands to the measured SM at a depth of 0–5 cm was optimized by random forest regression. According to the sensitive bands, SMMI and PDI from different feature spaces were constructed to explore their feasibility for monitoring SM under different land cover types. Second, fractional vegetation cover (FVC) in the study area was estimated by nine kinds of FVC estimation models and compared with the measured FVC. The effects of different FVC methods on estimating SM by MSMMI and MPDI were evaluated. The results show that red edge and short-wave infrared (SWIR) bands of Sentinel-2 had irreplaceable effects on the land cover classification. In terms of monitoring SM in bare soil areas, the SM indices with SWIR bands had high correlations with measured SM. For vegetation-covered areas, MSMMI from the FVCgr model (dimidiate pixel model with red edge bands) and the Short wave infrared1–Short wave infrared2 feature space had the highest correlation with the measured 0–5 cm depth SM. Whether vegetation-covered areas or bare soil areas, the combination of red edge and SWIR bands can effectively improve the estimation accuracy of SM. MSMMI can be used as the best SMMI in the study area. Sentinel-2 images, with great potential, can effectively estimate SM at a depth of 0–5 cm in farmland with complex environments.

Abstract Background Ischaemic stroke has become the main cause of death and severe neurological disorders, for which effective restorative treatments are currently limited. While stem cell transplantation offers therapeutic potential through neural regeneration, this approach is associated with the challenges of limited applicable sources. Hair follicle stem cells (HFSCs) are multipotential cells that can differentiate into ectodermal and mesodermal lineages and proliferate for long periods. The therapeutic potentials of HFSCs have not been investigated in ischaemic stroke models, and therefore, in this study, we aimed to determine whether they could survive and migrate to ischaemic areas after a stroke attack. Methods A rat model of middle cerebral artery ischaemia/reperfusion was established and intravenously administered HFSCs. The potential of HFSCs to migrate and differentiate into neuron-like cells as well as their ability to reduce the infarct size was evaluated. Rat brain tissue samples were collected 2 weeks after cell transplantation and analysed via TTC staining, immunofluorescence and immunohistochemistry methods. The data were statistically analysed and presented as the means ± standard deviations. Results Intravenously administrated rat HFSCs were able to migrate to the penumbra where they expressed neuron-specific markers, reduced the infarct volume and promoted neurological recovery. Conclusion HFSC transplantation has therapeutic potential for ischaemic stroke and is, therefore, worthy of further investigation toward possible clinical development for treating stroke patients.

Abstract Titanium dioxide nanomaterials are applied in numerous fields due to their splendid physicochemical characteristics, which in turn poses a potential threat to human health. Recently, numerous in vivo studies have revealed that titanium dioxide nanoparticles (TNPs) can be transported into animal brains after exposure through various routes. Absorbed TNPs can accumulate in the brain and may disturb neuronal cells, leading to brain dysfunction. In vitro studies verified the neurotoxicity of TNPs. The mechanisms underlying the neurotoxicity of TNPs remains unclear. Whether necroptosis is involved in the neurotoxicity of TNPs is unknown. Therefore, we performed an in vitro study and found that TNPs induced inflammatory injury in SH-SY5Y cells in a dose-dependent way, which was mitigated by necrostatin-1 (Nec-1) pretreatment. Since receptor-interacting protein kinase 1 (RIP1) is reported to be the target of Nec-1, we silenced it by siRNA. We exposed mutant and wild-type cells to TNPs and assessed inflammatory injury. Silencing RIP1 expression inhibited inflammatory injury induced by TNPs exposure. Taken together, Nec-1 ameliorates the neurotoxicity of TNPs through RIP1. However, more studies should be performed to comprehensively assess the correlation between the neurotoxicity of TNPs and RIP1.

Abstract Background This systematic review aimed to investigate whether non-surgical periodontal therapy (NSPT) can reduce systemic inflammatory levels and improve metabolism in patients undergoing haemodialysis (HD) and/or peritoneal dialysis (PD). Methods Electronic databases (PubMed, EMBASE, CENTRAL, CNKI, and WFPD) were searched for randomized controlled trials (RCTs) performed through July 2019. The risk of bias within studies was assessed with the Cochrane Collaboration’s risk assessment tool. The systemic inflammatory and metabolic outcomes included the highly sensitive C-reactive protein (hs-CRP), interleukin 6 (IL-6), tumour necrosis factor-a (TNF-a), the albumin (Alb), and lipid metabolite levels. Meta-analyses (MAs) were performed to calculate the overall effect size where appropriate. Results Five RCTs were included in this study. Compared with untreated periodontitis groups, the dialysis patients after NSPT significantly showed decreased hs-CRP levels at less than or equal to 2 months (standardized mean difference: − 1.53, 95% confidence interval − 2.95 to − 0.11). No significant difference was found in IL-6 and Alb levels following NSPT at either the 3- or 6- month follow-ups. No MAs could be performed on the TNF-a level and the lipid metabolic markers. Conclusions NSPT can moderately reduce serum hs-CRP levels in HD and/or PD patients, but did not significantly change IL-6 or Alb levels. For TNF-a and lipid metabolism markers, no sufficient evidence supports that these levels are changed after NSPT. Additional scientific research is necessary to assess the effects of NSPT on systemic inflammation and metabolic parameters in dialysis patients.

Abstract Bacteriophages, also known as phages, are specific antagonists against bacteria. T7 phage has drawn massive attention in precision medicine owing to its distinctive advantages, such as short replication cycle, ease in displaying peptides and proteins, high stability and cloning efficiency, facile manipulation, and convenient storage. By introducing foreign gene into phage DNA, T7 phage can present foreign peptides or proteins site‐specifically on its capsid, enabling it to become a nanoparticle that can be genetically engineered to screen and display a peptide or protein capable of recognizing a specific target with high affinity. This review critically introduces the biomedical use of T7 phage, ranging from the detection of serological biomarkers and bacterial pathogens, recognition of cells or tissues with high affinity, design of gene vectors or vaccines, to targeted therapy of different challenging diseases (e.g., bacterial infection, cancer, neurodegenerative disease, inflammatory disease, and foot–mouth disease). It also discusses perspectives and challenges in exploring T7 phage, including the understanding of its interactions with human body, assembly into scaffolds for tissue regeneration, integration with genome editing, and theranostic use in clinics. As a genetically modifiable biological nanoparticle, T7 phage holds promise as biomedical imaging probes, therapeutic agents, drug and gene carriers, and detection tools.

With the development of urbanization, the transportation network of underground tunnels has been gradually formed and improved. It is a complicated issue for engineering construction when two municipal road tunnels intersect at one point. Based on a construction site of the crossing point of the Huayuan Road Tunnel and Luzhou Road Tunnel in Hefei City, China, the finite element analysis method is used to calculate and analyze the deformation characteristics of the crossing point of the tunnels during the asymmetric construction of connecting parts. The deformation behaviors of the crossing point of tunnels subjected to symmetrical construction are also studied for comparison. Results show that the deformations of the supporting pile and tunnel frame structures increase rapidly when they are subjected to asymmetric construction, while the lateral movement of the supporting pile and the deformation of the tunnel structure can be greatly limited when the symmetrical construction method is adopted. Some suggestions for engineering construction are put forward to ensure the safety of the structure, such as multi-stage construction and temporary supporting measure.

There are many promising renewable energy (RE) technologies that could help increase the contribution of RE in energy supply but which are not yet commercially available. The development rate of new RE technologies depends on many factors, such as Research and Development (R&D) efforts and policies. This study focuses on comparing China’s efforts regarding the development of new RE technologies (e.g., wave and tidal, binary geothermal power, floating solar, micro hydro, osmotic energy, floating offshore wind and vertical axis wind turbines) with those of the European Union (EU). For this purpose, we collected data from publications and databases and analysed several indicators: e.g., the development of renewable electricity generation and capacity, demonstration projects, investments in R&D and patent applications. The results show that China has become a big player globally for mainstream renewable electricity (hydropower, wind and solar PV). This development is due to China’s industrial policy and prioritization of effectiveness over cost efficiency. The main developments in China occurred in the 2010s, while the EU was a frontrunner in the 2000s. For the newer or less mainstream technologies, the application in China is still low, compared to the EU, except for floating solar, where China is a lead player. Regarding patent applications, China has shown a higher application amount compared to the EU since 2006. However, only a small share of China’s patents are valid internationally. We conclude that China has emerged as a big player in mainstream renewable energy technologies over the last decade. In regard to new renewable energy technologies, China is predominantly involved in solar energy and, in comparison to the EU, less in other new technologies (e.g., binary geothermal systems and ocean energy).

Summary Ganoderic acids produced by Ganoderma exhibit anticancer and antimetastatic activities. A novel approach by combining Vitreoscilla haemoglobin (VHb) expression and calcium ion induction was developed to enhance ganoderic acid (GA) production in liquid static cultures of G. lingzhi. The maximum contents of GA‐O, GA‐S and GA‐Me were 1451.33 ± 67.50, 1431.23 ± 79.74 and 1283.81 ± 85.13 μg per 100 mg cell weight, respectively under the integrated approach, which are the highest contents as ever reported in Ganoderma. The contents of squalene and lanosterol were increased by 2.0‐ and 3.0‐fold in this case compared with those in the control. The transcription levels of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase, farnesyl‐diphosphate synthase, squalene synthase and cytochrome P450 CYP5150L8 were upregulated by 2.56‐, 3.31‐, 2.59‐ and 6.12‐fold respectively. Additionally, the expression of VHb improved the ratio of type I to type II GA in liquid static cultivation of G. lingzhi. The transcription levels of cyp512a2, cyp512v2 and cyp512a13, candidate cytochrome P450 genes involved in oxidative modification of the lanostane skeleton in GA biosynthesis, were also increased by 2.28‐, 2.65‐ and 3.54‐fold in the VHb‐expressing strain respectively. Our results illustrated that the approach described here efficiently improved GA production in G. lingzhi fermentation.

The ecological conditions in urban area are greatly changed during the process of industrialization and urbanization of China. The pressure-state-response (PSR) framework is the most popular method to evaluate the ecological quality by integrating a set of remote sensing and statistical indicators into one index through a weighting method. However, a completely remote-sensed ecological index (RSEI), integrating normalized difference vegetation index (NDVI), Wet, land surface temperature (LST), and the normalized differential build-up and bare soil index (NDBSI) through principal components analysis (PCA) method, has been proposed to assess the regional ecological quality. The publications about urban ecological evaluation by RSEI often focus on only one city or a certain area and there are few types of research on the ecological quality assessment by RSEI of 35 major cities in China. In this paper, we employed RSEI to monitor the changes in the ecological quality in China' 35 major cities. The results of RSEI were compared to that of PSR and stepwise regression method was applied to establish the quantitative relationship among RSEI, NDVI, Wet, NDBSI, and LST. The results show that there are 18 cities with ecological quality deteriorated, mainly located in the east and southwest of China (Shanghai, Guangzhou, Hongkong, Macao, Nanjing, Haikou, Shijiazhuang, and Xi'an), and 17 cities with better ecological quality, mainly located in the north and central area of China (Beijing, Tianjin, Shenzhen, Taipei, Fuzhou, Chongqing, and Jinan), from 1990 to 2015. The 3D-scatter plots of RSEI, NDVI, Wet, NDBSI, and LST demonstrate that the levels of very bad and bad mainly situate in where with a high density of built-up and low vegetation cover and soil water content. The PSR map, acquired from integrating 17 indicators, is quite similar to that of RSEI generated by merging only four remote-sensed indicators. This indicates that RSEI can be adopted to characterize regional ecological quality. Take the quantitative equation of Shanghai in 2015 as an example, every 1.46 decrement in NDBSI or each 3.72 increments in NDVI value can result in one increment in RSEI value and the ecological quality can be improved. Specifically, the expansion of the built-up area can lead to ecological degradation, and vegetation construction can promote eco-environmental quality.

Dalbergia odorifera T. Chen seedlings do not grow well in the typical red soils of tropical regions. Eighteen culture substances filled with different substrate combinations and proportions of red soil, coconut coir powder, deciduous leaf powder, and sand were used as to determine their effects on the growth, root system development, dry matter accumulation and allocation, leaf relative electrolyte leakage, chlorophyll content, root superoxide dismutase activity, root malondialdehyde content, and total soluble sugar content of D. odorifera. Results demonstrated that different substrate combinations and proportions had different effects on the performance of D. odorifera. All mixed substrates were better than any single substrate. The suitable substrate combinations and proportions of sand, coconut coir powder, and deciduous leaf powder mixed with red soil improved the growth, root architecture, and physiological characteristics of D. odorifera seedling. For example, groups C1-2 (coconut coir/red soil = 2/2, v/v, the same below) and C3-2 (red soil/sand = 2/2) exerted the best effects on plant growth and biomass accumulation. Groups C1-2, C2-2 (deciduous leaf powder/red soil = 2/2), and C3-2 remarkably enhanced root system development. Group C6 (coconut coir/red soil/sand = 1/1/1) substantially promoted root nodule development. Group C3-1 (red soil/sand = 3/1) exhibited the best effects on physiological characteristics. On the basis of the comprehensive evaluation of Euclid's multidimensional space mathematical model, we found that the suitable substrate combinations followed the order of C1-2 > C3-1 > C2-2. This research provides scientific guidance for the proper seedling culture of D. odorifera and the rational utilization of solid wastes such as coconut coir and deciduous leaves of Ficus elastica.

Aims. The over-the-scope clip (OTSC) has recently emerged as a new endoscopic device for treating gastrointestinal bleeding, perforations, fistulas, and leaks. A modified OTSC device (full-thickness resection device, FTRD) has been widely used for endoscopic full-thickness resection. However, there is less experience regarding the indications and methods for OTSC removal. We aimed to summarize the existing methods and indications for OTSC removal. Methods. We searched PubMed, Cochrane Library, and ClinicalTrials.gov to identify relevant publications on OTSC removal. The details of OTSC removal, including the methods, indications, success rates, adverse events, and failure causes, were extracted and summarized. A meta-analysis of pooled success rates was conducted using STATA 15.0. Results. Eighteen articles were included. The reported methods for OTSC removal included (1) grasping forceps, (2) the Nd : YAG laser, (3) argon plasma coagulation, (4) the remOVE system, (5) endoscopic mucosal resection/endoscopic submucosal dissection, and (6) ice-cold saline solution. Indications for OTSC removal were (1) poor healing, (2) OTSC misplacement, (3) repeat biopsy/therapy or further treatment, (4) adverse events after OTSC implantation, (5) removal after recovery, and (6) patient wishes. The pooled success rate of OTSC removal was 89% in patients treated with the remOVE system. Minor bleeding, superficial thermal damage, and superficial mucosal tears were common adverse events. Mucosal overgrowth was the main cause of OTSC removal failure. Conclusions. The remOVE system is the best investigated method, with sufficient efficacy and safety for OTSC removal. This is the first systematic review of OTSC removal and provides significant guidance for clinical practice.

Background: Uncoordinated 51-like kinase 1 (ULK1) plays a vital role in autophagy. ULK1 dysregulation has recently been found in several human cancers. Methods: mRNA expression levels of ULK1 and clinical information were analysed from The Cancer Genome Atlas data. ULK1 expression levels were verified in 36 paired fresh ccRCC tissue specimens by western blot analysis. Expression of ULK1 was knockdown by shRNA lentivirus. ULK1 activity was inhibited by SBI-0206965. The effect of inhibition of ULK1 was measured by detecting the apoptotic rate, autophagy, and the ratio of ROS and NADPH. The efficacy of SBI-0206965 in vivo was assessed by the murine xenograft model. Findings: ULK1 mRNA expression was significantly upregulated in clear cell renal cell carcinoma (ccRCC) and overexpression of ULK1 correlated with poor outcomes. We found that ULK1 was highly expressed in 66.7% of ccRCC tumours (p

Underground mining would cause ground subsidence damage and large amounts of cracks, which would result a loss of surface moisture and nutrient and intensifying drought. There are a few reports about damage to plant roots caused by coal mining. The irregular distribution of plant roots in soil and the different forces generated in process of surface subsidence are difficult to study comprehensively. The technologies to repair damaged plant roots have not been completely perfected yet. Based on quantitative simulation of alfalfa root cut-repair experiment, this paper discusses the influences of inoculated Arbuscular Mycorrhiza Fungi on alfalfa root and the mitigation effects of an inoculation on the growth of alfalfa. Root injured alfalfa were investigated by soil pot experiments. The result indicated that at the same cut degree, the growth situation of inoculated alfalfa is better than the contrast. Compared with the Olsen-P content, at cut level of 0 and 1/3, the sand of inoculated alfalfa has less Olsen-P than contrast, at cut degree of 1/2 and 2/3, the sand of inoculated alfalfa has more Olsen-P than contrast, at degree of 3/4, the sand of inoculated alfalfa has less Olsen-P than contrast, the change trend of Olsen-P content is concerned with the relative strength size of absorb Olsen-P by alfalfa root and dissolve Olsen-P by root exudates and hyphae interstate.

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium Tuberculosis (MTB). It is the second largest single cause of death besides novel coronavirus pneumonia. Along with the abuse of antibiotics and extensive use of anti-tuberculosis drugs, multidrug-resistant (MDR) TB, drug-resistant (XDR) TB and totally drug-resistant (TDR) TB became obstacles to the tuberculosis eradication worldwide. According to the World Health Organization (WHO) statistics, China is not only a high burden tuberculosis country in the world, but also a country with a serious epidemic of MDR. Traditional drugs fail to meet the needs of tuberculosis control. Therefore, it is urgent to find new targets of anti-tuberculosis drugs and develop new anti-tuberculosis drugs. Hence, this paper systematically summarizes the mechanism of traditional and newly developed anti-tuberculosis drugs, in which stressing the research progress of drug resistance mechanisms. This work provides us with new insights of new antituberculosis drug developments, and may contribute to a reduction in the harm that tuberculosis brings to society. [ABSTRACT FROM AUTHOR]