51. The Cannabinoid 1–Receptor Silent Antagonist O-2050 Attenuates Preference for High-Fat Diet and Activated Astrocytes in Mice
- Author
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Keiichi Irie, Makiko Ohji, Michihiro Fujiwara, Maiko Araki, Kenichi Mishima, Atsunori Shirakawa, Sei Higuchi, Shohei Mishima, Kiyoshi Matsuyama, Kenji Mishima, Yoshiharu Akitake, and Katsunori Iwasaki
- Subjects
Male ,medicine.medical_specialty ,Cannabinoid receptor ,medicine.medical_treatment ,Hypothalamus ,Biology ,Food Preferences ,Mice ,Receptor, Cannabinoid, CB1 ,Internal medicine ,medicine ,Animals ,Dronabinol ,Receptor ,Pyrans ,Pharmacology ,Mice, Inbred ICR ,lcsh:RM1-950 ,digestive, oral, and skin physiology ,Antagonist ,food and beverages ,Feeding Behavior ,Dietary Fats ,Endocannabinoid system ,Conditioned place preference ,lcsh:Therapeutics. Pharmacology ,Endocrinology ,medicine.anatomical_structure ,nervous system ,Astrocytes ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Cannabinoid ,hormones, hormone substitutes, and hormone antagonists ,Astrocyte - Abstract
Endocannabinoids have been shown to activate reward-related feeding and to promote astrocytic differentiation. We investigated whether high-fat diet (HFD) intake produced a preference for HFD via an endocannabinoid-dependent mechanism. In the conditioned place preference test, the 2-week HFD–intake group showed preference for HFD and had increased expression of a marker for reactive astrocytes, glial fibrillary acid protein (GFAP), in the hypothalamus. The cannabinoid CB1–receptor antagonist O-2050 reduced the preference for HFD and expression of GFAP in the hypothalamus. These results suggested that HFD intake led to the development of a preference for HFD via astrocytic CB1 receptors in the hypothalamus. Keywords:: preference for high-fat diet, endocannabinoid, astrocyte
- Published
- 2010