51. Validation of a SPE HPLC–UV method for the quantification of a new ER-specific photosensitizer OR-141 in blood serum using total error concept
- Author
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Olivier Feron, Joëlle Quetin-Leclercq, Emilie Bony, Robert Kiss, David Delvaux, Yohan Mace, Olivier Riant, and Adan Pinto
- Subjects
Serum ,Clinical Biochemistry ,Pharmaceutical Science ,Endoplasmic Reticulum ,01 natural sciences ,Analytical Chemistry ,Matrix (chemical analysis) ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Blood serum ,Pharmacokinetics ,Drug Discovery ,Calibration ,Animals ,Photosensitizer ,Solid phase extraction ,Chromatography, High Pressure Liquid ,Spectroscopy ,Photosensitizing Agents ,Chromatography ,Chemistry ,Solid Phase Extraction ,010401 analytical chemistry ,Reproducibility of Results ,Repeatability ,0104 chemical sciences ,Dilution ,030220 oncology & carcinogenesis - Abstract
The aim of this work is to develop the first validated HPLC-UV method quantification in blood serum for a new endoplasmic reticulum (ER)-specific benzophenazine photosensitizer (OR-141). A fast solid phase extraction (SPE) cleaning sample procedure was achieved on C18 encapped (ec) SPE cartridges and the separation was performed on a RP-18e column (5μM) using an isocratic elution with methanol. The method has been fully validated according to accuracy profiles based on total error and tolerance intervals. Calibration was performed in the matrix and trueness (
- Published
- 2017