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Additional file 1. Detailed methods for Anti-IFN-�� autoantibody assay, Bio-Plex��� 25 cytokine assay, flow cytometry, DNA extraction, DNA libraries and sequencing, and bioinformatics analysis are provided in Additional file.

Zhenming Yang,1,2 Wen Zeng,2 Ye Qiu,3 Guangnan Liu,4 Jianquan Zhang1 1Department of Respiratory and Critical Medicine, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, 518000, People’s Republic of China; 2Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China; 3Department of Comprehensive Internal Medicine, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China; 4Department of Respiratory Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530005, People’s Republic of ChinaCorrespondence: Jianquan ZhangDepartment of Respiratory and Critical Medicine, The Eighth Affiliated Hospital, Sun Yat-sen University, No. 3025, Shennan Middle Road, Shenzhen, 518000, Guangdong, People’s Republic of ChinaTel +8613978123845Fax +86755-23482484Email jqzhang2002@126.comBackground: Talaromyces marneffei (TM) is the only temperature-biphasic pathogen among Penicillium spp. that causes talaromycosis marneffei (TSM). Clinical manifestations include fever, cough, expectoration, superficial and deep lymph node enlargement, hepatosplenomegaly, subcutaneous nodules, and bone and joint infections. Cases of TSM in Hodgkin lymphoma (HL) patients are uncommon. The clinical manifestations and imaging findings are similar in TSM and HL, which make it difficult for clinicians to distinguish between TSM and HL. Both diseases can present with symptoms, can involve the blood or the respiratory system and can include other symptoms. We report a rare case of HIV-negative nodular sclerosing Hodgkin lymphoma (NSHL) combined with T. marneffei infection to improve clinical knowledge.Case Presentation: The patient was a 51-year-old man who presented with a 1-month history of cough, expectoration, intermittent fever in the afternoon and night, cervical lymph node enlargement, diabetes and previous lung surgery. He had markedly elevated serum inflammatory markers and moderate diffuse lung dysfunction. Chest computed tomography (CT) showed diffuse nodular lesions in both lungs with mediastinal lymph node enlargement. The patient did not respond to antibacterial and diagnostic antituberculosis therapy. After lymph node biopsy and lung culture, we obtained a definite diagnosis of NSLH with T. marneffei infection and administered antifungal therapy. His symptoms improved, and he was discharged for further treatment. Unfortunately, he died of Salmonella sepsis 7 months later.Conclusion: It is rare for NSLH patients to be infected with T. marneffei. Both diseases can present with fever, lymphadenopathy, and hepatosplenomegaly and involve the blood and respiratory system or can cause other symptoms. Clinically, a misdiagnosis or missed diagnosis may occur. A multisite biopsy or culture should be performed to make a definitive diagnosis. Early antifungal therapy combined with standard chemotherapy can achieve satisfactory clinical efficacy.Keywords: Talaromyces marneffei, nodular sclerosing Hodgkin lymphoma, identification, treatment

Chaetarthria indica d���Orchymont, 1920 Material examined. 3 males, 2 females, 2 unsexed spec. (SYSU), China, Guangdong, Taishan County, Shangchuan Island, nature reserve for Macaca mulatta, elev. 20 m, 21˚46��01˝N, 112˚49��02˝E, 19.I.2019, Fenglong Jia, Zulong Liang, Shenyu Miao leg. 1 male, China, Guangdong, Shenzhen City, Mt. Wutong, Taishanjian, 22˚35��50.94˝N, 114˚10��47.96˝E, mixed leaf litter, sifted, elev. 248 m, 18.XI.2018, Cheng, Shuai & Zhao leg. Ecology. Within the specimens from Shangchuan Island, 1 male and 1 female were collected in a shallow pool with fine sand covered by leaf litter, and 1 male and other 4 specimens were collected under stones in river near edge. The specimen from Mt. Wutong was from leaf litter with wet ground., Published as part of Jia, Fenglong, Yang, Zhenming, Jiang, Lu, Chen, Kai & Jiang, Shujiao, 2020, Chaetarthria chenjuni Jia & Yang, sp. nov. (Coleoptera: Hydrophilidae), a new species from China and additional faunistic records, pp. 146-149 in Zoological Systematics 45 (2) on page 149, DOI: 10.11865/zs.202019, http://zenodo.org/record/4617214

Chaetarthria chenjuni Jia & Yang, sp. nov. (Figs 1–7) Type material. Holotype. male (SYSU), China, Hainan, Wuzhishan Mt., Guanshandian, 18˚53´N, 109˚41´E, elev. 650 m, 21.IV.2012, Yin Ziwei leg. Paratypes (3 spec. IZCAS; 2 spec. NMPC; 2 spec. SHNU; 6 spec. SYSU). 5 spec. same data as holotype; 1 male, 1 female, China, Hainan, Lingshui County, Diaoluoshan Mt., Winding Road, 18˚42´N, 109˚52´E, elev. 600–1000 m, 28.IV.2012, Peng & Dai leg.; 2 females (IZCAS: IOZ(E)20022538, IOZ(E)20022533), China, Guangxi, Napo, Defu, elev. 1440 m, 3.IV.1998, Wenzhu Li leg. (transcribed from Chinese); 1 female (IZCAS: IOZ(E)20022536), locality as above, elev. 1300 m, 14.VIII.1998, Tongli He leg. (transcribed from Chinese); 3 females, China, Guangdong, Shenzhen, Dapeng Peninsula, Getian Village, 22.48157°N, 114.62643°E, 2.VIII.2019, Jia & Mai leg. (transcribed from Chinese). Diagnosis. This new species may be close to C. indica d'Orchymont, 1920. It can be distinguished from C. indica by its labrum black, elytra uniformly black, maxillary palps piceous, ventral surface black, legs black with tarsomeres dark brown, elytra with distinct punctures as well as the systematic punctures, an arrangement otherwise only known from C. saundersi d'Orchymont in the Oriental Region; aedeagus with median lobe broadly rounded apically, without a flagellum arising out of the middle of anterior margin, gonopore oval (Fig. 7). General description. Labrum same colour as clypeus, maxillary palps uniformly dark reddish brown, pronotum with posterior angles dark brown, only slightly lighter than disc, elytra uniformly black. Ventral surface black; legs black, with tarsomeres dark brown. Head and pronotum without punctures. Pronotum without distinct anterior angles, posterior angles acute. Elytra with 4 distinct regular series of punctures, between regular series of punctures with 2 distinct irregular series of punctures, lightly finer than systematic punctures, lateral portion with irregular punctures, coarser than those on disc. Aedeagus with phallobase ca. 1.6× as long as parameres, median lobe broadly rounded apically, without a flagellum, gonopore situated apically. Description. Size and Form. 2.4–2.6 mm. Broadly oval, strongly convex (Fig. 1). Elytra slightly longer than wide. Colour. Dorsum black. Labrum same colour as clypeus or slightly lighter, without lighter anterior margin. Maxillary palps uniformly dark reddish brown. Pronotum with posterior angles dark brown (Jia et al., 2018: fig. 3a), only slightly lighter than disc. Elytra uniformly black (Figs 1, 3). Ventral surface black. Mentum dark brown. Legs black with slightly lighter tarsomeres; epipleura dark brown. Head. Ground punctation on labrum, clypeus and frons undetectable (Fig. 4). Systematic punctures on labrum, frons and clypeus very fine but detectable. Labrum not emarginate anteriorly. Eyes small, slightly protruding, separated by ca. 5.5 × width of one eye. Maxillary palps about a half as long as width of head, apical palpomere almost symmetrical and longer than penultimate (Jia et al., 2018: fig. 3d). Labial palps about one-third as long as width of mentum, apical palpomere conical, shorter than 2nd, 2nd palpomere with numerous long setae on inner and outer face. Antennae 8-segmented, scape almost as long as following antennomeres combined, club loosely segmented. Mentum (Jia et al., 2018: fig. 3d) longer than wide, quadrate, entire anteriorly; with very fine and scattered punctures anteriorly, not punctate medially. Thorax. Pronotum shinning, without distinct anterior angles, lateral margin of anterolateral angle dilated, posterior angles acute (Fig. 6); lateral stria present along entire lateral margin, terminating posteriorly at hind angle. Pronotal ground punctation undetectable, systematic punctures very fine but detectable. Elytra smooth and shining, with 4 distinct regular series of punctures (Fig. 5) between regular series of punctures with a few irregular series of punctures (Fig. 3), lateral portion with irregular punctures that are coarser than those on disc. Sutural stria reaching half of elytral length (Jia et al., 2018: fig. 3c), no series of punctures before sutural stria. Prosternum extremely short, only slightly convex, not carinate. Mesosternum glabrous, slightly concave, with a median, transverse and lightly arcuate ridge at level of anterior mesocoxal margin. Metasternum slightly raised, a little more convex and sparsely pubescent medially. Metepisterna about 4.3× as long as wide, more densely pubescent than metasternum, almost parallel sided except posteriorly, where inner margin bends smoothly towards outer margin. Procoxae densely pubescent, meso- and metacoxae glabrous. Femora with sharply defined tibial grooves on inner face; Femora stout. Pro- and meso-femora densely pubescent except on apical extremity; anterior half of metafemora densely pubescent, posterior half glabrous with some coarse punctures (Jia et al., 2018: fig. 3b), posterodorsal margin densely pubescent. Tibiae slender, hardly flattened. Tarsi with 5 tarsomeres, first metatarsomere shorter than second. Abdomen. Ventrites 1–2 with a large cavity on each side, filled with a hyaline mass supported and covered by a fringe of long stiff yellow setae rising from anterior margin of basal ventrite (Fig. 2); first two ventrites carinate medially. Aedeagus. Aedeagus with phallobase ca. 1.6× as long as parameres; parameres narrowly rounded apically; median lobe distinctly broader than parameres medially, broadly rounded apically, without a flagellum, gonopore situated apically (Fig. 7). Etymology. The species is named after Dr. Jun Chen, a specialist of mites from Institute of Zoology, Chinese Academy of Sciences, Beijing, China, who kindly invited the senior author to examine the specimens in IZCAS. Distribution. China (Hainan, Guangxi, Guangdong).

Jia, Fenglong, Yang, Zhenming, Jiang, Lu, Chen, Kai, Jiang, Shujiao (2020): Chaetarthria chenjuni Jia & Yang, sp. nov. (Coleoptera: Hydrophilidae), a new species from China and additional faunistic records. Zoological Systematics 45 (2): 146-149, DOI: 10.11865/zs.202019, URL: http://zoobank.org/485dba90-162e-414a-b975-79cf0cfa5ce1

Chaetarthria almonara Knisch, 1924 Material examined. 1 male (SYSU), China, Yunnan, Xishuangbanna, Yexianggu, sifting, 22˚10��55˝N, 100˚51��58˝E, elev. 950 m, 27.III.2018, Peng, Shen & Cheng leg., Published as part of Jia, Fenglong, Yang, Zhenming, Jiang, Lu, Chen, Kai & Jiang, Shujiao, 2020, Chaetarthria chenjuni Jia & Yang, sp. nov. (Coleoptera: Hydrophilidae), a new species from China and additional faunistic records, pp. 146-149 in Zoological Systematics 45 (2) on page 149, DOI: 10.11865/zs.202019, http://zenodo.org/record/4617214

No genomic sequence of Mycobacterium isolated from orchids has been reported yet; therefore, this study intends to analyze the complete genomic sequence of a growth-promoting Mycobacterium from orchid Doritaenopsis. Mycobacterium strain Mya-zh01 was isolated from the flower stalk of Doritaenopsis Jiuhbao Red Rose. Our results show that Mya-zh01 can effectively produce and secrete the plant growth hormone indole-3-acetic acid (IAA). Inoculation of Mya-zh01 increased root number and length, plant height, leaf number, and leaf length in Doritaenopsis. Furthermore, inoculation of Mya-zh01 promotes seed germination in Doritaenopsis. We sequenced and assembled chromosome for Mya-zh01 (5,027,704 bp with 68.48% GC content), which was predicted to encode 4968 proteins with functions in oxidation reduction, growth, plasma membrane, ATP and DNA binding, carbon metabolism and biosynthesis of amino acids pathways. Mya-zh01 may trap iron from nature or host cells to facilitate the growth of the orchids by producing two siderophores (Mycobactin and Nocobactin NA). Four pathways (tryptamine, indole-3-acetamide, indole-3-pyruvate, and flavin monooxygenase) and seven enzymes [tryptophan synthase alpha chain, tryptophan synthase beta chain, amidase, monoamine oxidase, indole-3-pyruvate monooxygenase, indole-3-pyruvate decarboxylase and aldehyde dehydrogenase (NAD +)] involved in IAA biosynthesis were predicted in Mya-zh01 genome. In conclusion, this study demonstrated the significance of Mya-zh01 in facilitating plant growth and seed germination in Doritaenopsis by IAA biosynthesis, which provides a new insight into the mechanism of plant–bacteria interaction in Doritaenopsis.