101. Intestinal colonization traits of pandemic multidrug-resistant Escherichia coli ST131
- Author
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Dimitrios Vagenas, Mark A. Schembri, Makrina Totsika, Melanie L. Hutton, Rinaldo Ruter, Sohinee Sarkar, Stephanie Schüller, and Dena Lyras
- Subjects
0301 basic medicine ,E. coli ST131 ,030106 microbiology ,Fimbria ,Mutant ,fimH ,Clone (cell biology) ,Biology ,medicine.disease_cause ,Bacterial Adhesion ,law.invention ,Microbiology ,Cell Line ,03 medical and health sciences ,Probiotic ,Major Articles and Brief Reports ,Mice ,law ,multidrug resistance ,Drug Resistance, Multiple, Bacterial ,medicine ,Escherichia coli ,Immunology and Allergy ,Animals ,Humans ,Colonization ,Escherichia coli Infections ,Bacteria ,Epithelial Cells ,intestinal colonization ,Mucus ,Bacterial Load ,3. Good health ,Multiple drug resistance ,Intestines ,Infectious Diseases ,type 1 fimbriae ,Fimbriae, Bacterial ,Female ,Caco-2 Cells - Abstract
A detailed investigation into the intestinal lifestyle of Escherichia coli multilocus sequence type 131 revealed that these globally dominant multidrug-resistant pathogens use type 1 fimbriae to effectively colonize the mammalian gut and persist long term., Background Epidemiological studies point to the gut as a key reservoir of multidrug resistant Escherichia coli multilocus sequence type 131 (ST131), a globally dominant pathogenic clone causing urinary tract and bloodstream infections. Here we report a detailed investigation of its intestinal lifestyle. Methods Clinical ST131 isolates and type 1 fimbriae null mutants were assessed for colonization of human intestinal epithelia and in mouse intestinal colonization models. Mouse gut tissue underwent histologic analysis for pathology and ST131 localization. Key findings were corroborated in mucus-producing human cell lines and intestinal biopsy specimens. Results ST131 strains adhered to and invaded human intestinal epithelial cells more than probiotic and commensal strains. The reference ST131 strain EC958 established persistent intestinal colonization in mice, and expression of type 1 fimbriae mediated higher colonization levels. Bacterial loads were highest in the distal parts of the mouse intestine and did not cause any obvious pathology. Further analysis revealed that EC958 could bind to both mucus and underlying human intestinal epithelia. Conclusions ST131 strains can efficiently colonize the mammalian gut and persist long term. Type 1 fimbriae enhance ST131 intestinal colonization, suggesting that mannosides, currently developed as therapeutics for bladder infections and Crohn’s disease, could also be used to limit intestinal ST131 reservoirs.
- Published
- 2018