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To study the triterpenoid saponins in the whole plant of Lysimachia davurica Ledeb.Column chromatography (including AB-8 macroporous resin, silica gel and ODS) was used to separate triterpenoid saponins whose structures were elucidated by ESI-MS, NMR (1D and 2D) and hydrolysis methods.Two new triterpenoid saponins were isolated and established as 3beta, 16alpha, 28-trihydroxy-olean-12-en-3-0-(beta-D-glucopyranosyl-(1 --2)-beta-D-glucuronopyranosyl)-28-0-beta-D-glucopyranoside) (I), 3beta, 16alpha, 28-trihydroxy-olean-12-en-3-O-(beta-D-glucopyranosyl-(1 --2)-beta-D-glucuronopyranosyl)-28-0-beta-D-glucopyranoside) (II).Compounds I and II are new compounds and named as davuricosides D and J.

To establish a method for the germplasm preservation of R. glutinosa.Plantlets of different cultivars obtained by tip culture were inoculated into test tubes with MS medium supplemented with 0.2 mg.L-1 BA and 0.02 mg.L-1 NAA, and preserved at 4-6 degrees C in dark. At the same time, different media (A. distilled water + 10 g.L-1 agar; B. 1/2 MS + 5 g.L-1 agar; C. MS + 10 g.L-1 agar; D. 1/2 MS + 0.5 BA + 0.02 NAA + 10 g.L-1 agar; E. MS + 0.5 BA + 0.02 NAA + 10 g.L-1 agar) were set to conserve plantlets of "85-5" on the same condition.4 months later, the death rate of "Xinggeda" was 73%, "Tucheng" 60%, "85-5" 33%, and "Beijing 1" 9%. All of the plantlets of "85-5" in different media kept alive. 10 months later, most of the preserved plantlets browned and wilted except those on medium A.The low temperature endurance of R. glutinosa is cultivar-dependent. Medium A can preserve "85-5" for more than 10 months in vitro.

The ethyl acetate fraction obtained from the extraction of Artemisia annua yielded 11 flavones, four flavone glycosides and two chromene derivatives. Three new compounds were isolated: quercetagetin 4′-methyl ether, 2,2-dihydroxy-6-methoxychromene and 2,2,6-trihydroxychromene.

To study the bioactive constituents, quinic acid esters, from Siphonostegia chinensis.The compounds were extracted with solvents, isolated by various column chromatography and identified by spectroscopic methods.Three quinic acid esters were isolated and identified as 3,4-di-O-caffeoylquinic acid(I), macranthoin F(II), and methyl ester of 3, 4,5-tri-O-caffeoylquinic acid (III).All compounds were isolated from Siphonostegia for the first time.

To evaluate the germplasm of Rehmannia glutinosa on the basis of photosynthetic pigment contents (PPC).20 cultivars were planted on the same condition. On Oct. 23 and Sept. 25, 3 leaves per cultivar were collected on different plants, and 80 mg mesophyll was collected among upper lateral veins and was ground in 96% alcohol, and the supernatant was subjected to measure on a spectrophotometer (Angilent 8453).The PPCs among cultivars were significantly different at a Por = 0.01 level. The results of the measurements were similar. Chlolophyll a was the most abundant pigment, but varied to a great extent among different cultivars. 20 cultivars were divided into 9 homogeneous groups according to the contents of chlorophyll a by Duncan's multiple range test at Por = 0.05. In addition, the content of chlorophyll a was closely related to leaf color. The cultivars with higher chlolophyll a had deep green leaves, and those with lower had yellow green or pale green leaves.PPC was an inherent character and an important index for the germplasm evaluation of R. glutinosa.

To study the water soluble constituents from Amomum villosum.The constituents were separated and purified with chromatographic methods, identified by NMR, MS, UV and IR.Two quercetin glycosides: quercitrin (quercetin-3-O-alpha-L-rhamnoside I) and isoquercitrin (quercetin-3-O-beta-D-glucoside II) were isolated and identified.I and II were isolated for the first time from A. villosum.

To determine the contents of 6-Gingerol in Rhizoma Zingiberis Recens.HPLC method was used, with Alltech C18 column, acetonitrile-methol-water (43:5:52) as mobile phase with a flow rate of 0.8 mL.min-1, detecting wavelength 280 nm, and column temperature 35 degrees C.Retained time of 6-gingerol was near 19 min, showing a good recovery (98.2%) and linear correlation (r = 0.9999). The contents of 6-gingerol were 1.35-2.87 mg.g-1, and the water contents were 70.4-85.5% mL.g-1 in Rhizoma Zingiberis Recens.The method is appropriate for the determination of 6-gingerol in Rhizoma Zingiberis Recens. Gingerol can be used as a chemical marker of the quality control of Rhizoma Zingiberis Recens.

To provide basis for environmental factors of genuine crude Chinese angelica.On-the-spot investigation and indoor chemical analysis were made to study the physicochemical properties of cultivated soil of Chinese angelica.The physical properties and the organic matte and mineral nutrition of cultivated soil of Chinese angelica are best in Min County, Gansu Province. The ecological environment is the leading factor in forming genuine crude Chinese angelica.

To study the chemical constituents from the whole plant of Lysimachia davurica.The constituents were separated and purified by column chromatograph, and identified by spectral analysis and physical data.Six compounds were isolated and identified as triacontanoic acid(I), palmitic acid(II), beta-amyrin(III), stigmasterol(IV), oleanolic acid(V), soya-cerebroside I (VI).I, III, VI were isolated from this gene for the first time.

To investigate the chemical constituents of Securidaca inappendiculata.Compounds were isolated by silica gel column chromatography and Medium Pressure Liquid Chromatography, respectively. Structures of the compounds were elucidated by chemical evidence and spectral (UV, IR, MS, 1HNMR and 13CNMR) analysis.Eight compounds (1-8) were isolated and identified. Seven of them (2-7) are known compounds: oleanolic acid, p-hydroxytruxinic acid, p-coumaric acid, ethyl p-methoxycinnamate, uridine, beta-sitosterol and daucosterol. One (1) is a new hemiterpenoic acid glycoside, named securiterpenoside.A new hemiterpenoic acid glycoside, named securiterpenoside, was isolated from Securidaca inappendiculata Hassk. Seven known compounds were isolated from genus Securidaca for the first time.

A new method using gradient elution of reversed-phase high performance liquid chromatography was set up for the separation and determination of 12 natural phenolic acids simultaneously. The separation conditions were studied with a Zorbax ODS (5 microns) column, and optimized conditions were suggested with the mobile phase gradient elution of CH3CN-H2O (with 0.5% HAc) (5:95--35:65, V/V, in 40 min) at room temperature, flow rate at 1.0 mL/min and detection wavelength at 280 nm. And the effects of acidity on separations and the relationships between the phenolic acids structures and their retention behaviors were discussed. Qualitative and quantitative analysis of the 12 compounds were performed. The regression equation, correlation coefficient, linear range, detection limit and relative standard deviation (RSD) of each compound were obtained under the optimized conditions. The correlation coefficients of the 12 compounds were between 0.9980 and 0.9999. The detection limits ranged from 0.96 ng to 4.10 ng and RSDs wereor = 2.64%. These results demonstrate that the optimized determination method obtained is successful for the separation and determination of the 12 phenolic acids analysed.

Canthin-6-one, 11-hydroxycanthin-6-one, 11-methoxycanthin-6-one, 5-methoxycanthin-6-one, 4-hydroxy-5-methoxycanthin-6-one and canthin-6-one-3 N -oxide have been isolated from cell suspension cultures of Brucea javanica . The total yield of alkaloid produced in the cells and the medium is in excess of 2.0 mg/g dry weight of cells.

Cell suspension cultures developed from Artemisia annua exhibited antimalarial activity against Plasmodium faldparum in vitro both in the n-hexane extract of the plant cell culture medium and in the chloroform extract of the cells. Trace amounts of the antimalarial sesquiterpene lactone artemisinin may account for the activity of the n-hexane fraction but only the methoxylated flavonoids artemetin, chrysoplenetin, chrysosplenol-D and cirsilineol can account for the activity of the chloroform extract. These purified flavonoids were found to have IC50 values at 2.4 - 6.5 × 10(-5)M against P. falciparum in vitro compared with an IC50 value of about 3 × 10(-8)M for purified artimisinin. At concentrations of 5 × 10(-6)M these flavonoids were not active against P. falciparum but did have a marked and selective potentiating effect on the antiplasmodial activity of artemisinin.

Aerial parts of Ageratina deltoidea yielded 13 methoxylated flavonoids which were identified using UV, MS and 1 H NMR, and with reference to data given in the literature.

Phytochemical studies on aerial parts of Kalanchoe gracilis resulted in the isolation of nine flavonoids and one coumarin. Among the nine flavonoids, four new flavonoids were identified as eupafolin-rhamnosides. The five known compounds were determined as luteolin, quercetin, quercitrin, haempferol, and eupafolin, based on spectroscopic analysis

Cell suspension cultures of Brucea javanica (L.) Merr. were used to determine culture growth characteristics and production of canthin-6-one alkaloids. The major alkaloids produced were canthin-6-one, 11-hydroxycanthin-6-one, 5-methoxycanthin-6-one and 11-methoxycanthin-6-one. Alkaloids were synthesised throughout the 36 day growth cycle of the cells with maximum amounts within the cells occurring between days 20 to 28; approximately 10% of the alkaloids were in the medium at day 24 rising to 45% at day 32.