274 results on '"Sui, Qun"'
Search Results
102. ZEB1 promotes invasion and metastasis of endometrial cancer by interacting with HDGF and inducing its transcription
- Author
-
Yan-Yi, Xiao, Li, Lin, Yong-Hao, Li, Hui-Ping, Jiang, Li-Tong, Zhu, Yuan-Run, Deng, Dan, Lin, Wei, Chen, Cheng-Ying, Zeng, Li-Jing, Wang, Shao-Cheng, Chen, Qing-Ping, Jiang, Chun-Hua, Liu, Wei-Yi, Fang, and Sui-Qun, Guo
- Subjects
Original Article - Abstract
Zinc finger E-box binding homeobox 1 (ZEB1), as a typical transcription inhibitory factor of E-cadherin, plays a major role in stimulating the invasion and metastasis of tumors via modulating the epithelial-mesenchymal transition (EMT) signal. However, its function and modulatory mechanisms in endometrial carcinoma (EC) remain unclear. In this study, silencing ZEB1 significantly reduced EC cell migration, invasion, and metastasis, as well as enhanced the sensitivity of EC cells to cisplatin (cDDP) in vitro and in vivo. Mechanism analysis indicated that ZEB1 interacts with hepatoma-derived growth factor (HDGF) and co-localizes in the nucleus. In addition, ZEB1 as a transcription factor binds to the promoter of HDGF to stimulate HDGF transcription. Furthermore, suppression of HDGF in ZEB1-overexpressed EC cells not only reduced the expression of β-catenin, TCF4, and ZEB1, but also repressed β-catenin translocation from the cytoplasm into the nucleus and further downregulated the combination with TCF4. Interestingly, the β-catenin/TCF4 signaling feedback stimulates ZEB1 transcription and therefore constitutes a positive feedback loop. In clinical samples, ZEB1 positively correlates with HDGF expression, and co-expression of ZEB1 and HDGF promotes the pathogenesis of EC. In summary, our study demonstrated that the positive feedback loop of ZEB1/HDGF/β-catenin/TCF4 plays an unfavorable role in the metastasis of endometrial carcinoma.
- Published
- 2019
103. Separation and configurational assignment of stereoisomeric phenalenones from the marine mangrove-derived fungus Penicillium herquei MA-370
- Author
-
Attila Mándi, Bin-Gui Wang, Xin Li, Sui-Qun Yang, Sándor Balázs Király, Xiaoming Li, Hui Liu, and Tibor Kurtán
- Subjects
Stereochemistry ,Angiotensin-Converting Enzyme Inhibitors ,Fungus ,Peptidyl-Dipeptidase A ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,Drug Discovery ,Binding site ,Molecular Biology ,Density Functional Theory ,Marine fungi ,chemistry.chemical_classification ,Dose-Response Relationship, Drug ,Molecular Structure ,biology ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Penicillium ,Absolute configuration ,Stereoisomerism ,Phenalenes ,Penicillium herquei ,biology.organism_classification ,0104 chemical sciences ,Chiral column chromatography ,010404 medicinal & biomolecular chemistry ,Enzyme ,Chirality (chemistry) - Abstract
Eight phenalenone derivatives, including four new compounds, aceneoherqueinones A and B (1 and 2), (+)-aceatrovenetinone A (3a), and (+)-aceatrovenetinone B (3d), along with four known congeners, (–)-aceatrovenetinone A (3b), (–)-aceatrovenetinone B (3c), (–)-scleroderolide (4a), and (+)-scleroderolide (4b), were characterized from the marine mangrove-derived fungus Penicillium herquei MA-370. Among them, compounds 1 and 2 are rare phenalenone derivatives featuring cyclic ether unit between C-5 and C-2′. All of these compounds were subjected to chiral HPLC analysis, and the unstable stereoisomers 3a–3d, containing configurationally labile chirality centers, were characterized by online HPLC-ECD measurements supported with TDDFT-ECD calculations. The structures of these compounds were elucidated by detailed analysis of their NMR and mass spectroscopic data, and the absolute configuration of compound 1 was confirmed by X-ray diffraction analysis, while those of compounds 2 and 3a–3d were determined by TDDFT-ECD calculations of their ECD spectra. All of the isolated compounds were tested for the inhibitory activity against angiotensin-I-converting enzyme (ACE), and compounds 1 and 2 displayed activity with IC50 values 3.10 and 11.28 μM, respectively. The intermolecular interaction and potential binding sites of 1 and 2 with ACE were elaborated by molecular docking, showing that compound 1 bound well with ACE via hydrogen interactions with residues Ala261, Gln618, Trp621, and Asn624, while compound 2 interacted with residues Asp358 and Tyr360.
- Published
- 2021
104. A new steroid with 7β,8β-epoxidation from the deep sea-derived fungus Aspergillus penicillioides SD-311.
- Author
-
Chi, Lu-Ping, Yang, Sui-Qun, Li, Xiao-Ming, Li, Xiao-Dong, Wang, Bin-Gui, and Li, Xin
- Subjects
- *
X-rays , *STEROIDS , *DRUG design , *MASS spectrometry , *RESEARCH funding , *DESCRIPTIVE statistics , *MOLECULAR structure , *CHROMATOGRAPHIC analysis , *ASPERGILLUS - Abstract
7β,8β-epoxy-(22E,24R)-24-methylcholesta-4,22-diene-3,6-dione (1), a new steroid, along with five known analogues (2–6), was isolated from the deep sea-derived fungus, Aspergillus penicillioides SD-311. Strikingly, 1 possessed a rare 7,8-epoxidation moiety. Meanwhile, this is the first time to report natural products from this fungus species. The structures were established by extensive spectroscopic analysis. The absolute configuration was determined by X-ray diffraction experiments. Compound 1 showed antibacterial activity against Vibrio anguillarum with MIC value of 32.0 µg/mL, while 2 displayed inhibitions against Edwardsiella tarda and Micrococcus luteus with MIC values both of 16 µg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
105. New citrinin analogues produced by coculture of the marine algal-derived endophytic fungal strains Aspergillus sydowii EN-534 and Penicillium citrinum EN-535
- Author
-
Yang, Sui-Qun, Li, Xiao-Ming, Li, Xin, Li, Hong-Lei, Meng, Ling-Hong, and Wang, Bin-Gui
- Published
- 2018
- Full Text
- View/download PDF
106. Research on Environmentally Friendly Chemical Technology for Green Reusable and Sustainable Water Metal Copper Ions
- Author
-
Yu-Lin Yang, Ching-Yu Peng, Shinn-Dar Wu, Meng-Ting Hsieh, Ting-Yu Chang, Yea-Chyi Lin, and Sui-Qun He
- Subjects
Metal ,Chemical technology ,Materials science ,chemistry ,Waste management ,visual_art ,visual_art.visual_art_medium ,chemistry.chemical_element ,Environmentally friendly ,Copper - Abstract
In traditional chemical processes, they are considered highly polluting and unfriendly to the environment. The air, water, soil, and land were severely damaged. Among the international environmental protection issues, especially the development of industry is a very worthwhile study. Therefore, this study will discuss metal ions in water. This study takes copper as the object of discussion. In the future, different metals, such as nickel, iron, zinc, and other metals, will be discussed, and industrial process problems will be solved. For copper, it is also a common metal in life. Copper metal bottles, handicrafts, circuit boards, heat dissipation components, metal soldering agents, sewage treatment agents and many other uses. However, in the copper process, electroplating, and waste after use are often treated as secondary pollution. From the perspective of technology and process, an environmentally friendly chemical process is proposed, which can also make the best use of reactants and waste water. After the process improvement, the copper ions are post-processed to extract new copper compound material technology. At the same time, the water body is controlled at pH 7, which can be reused more to achieve the goal of sustainable circulation. Thank partially support by the MOST 108-2221-E-343-001.
- Published
- 2020
107. [Expression of phosphoglycerate kinase 1 in endometrial carcinoma and its association with patients' outcome]
- Author
-
Li, Lin, Qing-Ping, Jiang, Dan, Lin, Wei, Chen, Hui-Ping, Jiang, Chun-Hua, Liu, Yan-Yi, Xiao, Li-Tong, Zhu, and Sui-Qun, Guo
- Subjects
Phosphoglycerate Kinase ,临床研究 ,Lymphatic Metastasis ,Humans ,Female ,Kaplan-Meier Estimate ,Prognosis ,Endometrial Neoplasms ,Neoplasm Staging - Abstract
OBJECTIVE: To investigate the expression of phosphoglycerate kinase 1 (PGK1) and its prognostic value in endometrial carcinoma (EC). METHODS: The expression of PGK1 was detected immunohistochemically in 30 normal endometrium and 130 EC specimens. The relationship between PGK1 protein expression and the clinicopathological features of the patients was evaluated. RESULTS: Immunohistochemical analysis revealed low PGK1 expression in 55.4% (72/130) and high PGK1 expression in 44.6% (58/130) of the EC specimens, as compared with the rates of 90% (27/30) and 10% (3/30) in normal endometrium, respectively (P < 0.001). PGK1 expression was significantly correlated with FIGO stage (P < 0.001), histological grade (P=0.002) and lymph node metastasis (P < 0.001). Kaplan-Meier survival analysis indicated that patients with a high PGK1 expression had a shorter overall survival rate than those with a low PGK1 expression (P < 0.001). Multivariate analysis showed that a high PGK1 expression was not the independent predictor of the prognosis of EC (P=0.077). CONCLUSION: A high expression of PGK1 is associated with aggressive and metastatic behaviors of EC, and detection of PGK1 provides assistance in evaluating the prognosis of patients with EC.
- Published
- 2018
108. Two New Diketomorpholine Derivatives and a New Highly Conjugated Ergostane-Type Steroid from the Marine Algal-Derived Endophytic Fungus Aspergillus alabamensis EN-547
- Author
-
Bin-Gui Wang, Sui-Qun Yang, Xiaoming Li, Lu-Ping Chi, and Xin Li
- Subjects
Aquatic Organisms ,Aspergillus alabamensis ,Magnetic Resonance Spectroscopy ,Ergostane ,Stereochemistry ,Morpholines ,medicine.medical_treatment ,Carboxylic acid ,Pharmaceutical Science ,Microbial Sensitivity Tests ,Crystallography, X-Ray ,01 natural sciences ,Article ,algal-derived endophytic fungus ,Steroid ,chemistry.chemical_compound ,Ergosterol ,Drug Discovery ,medicine ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,lcsh:QH301-705.5 ,diketomorpholine ,steroid ,antimicrobial activity ,chemistry.chemical_classification ,Aspergillus ,Bacteria ,Molecular Structure ,biology ,010405 organic chemistry ,Nuclear magnetic resonance spectroscopy ,biology.organism_classification ,Antimicrobial ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,lcsh:Biology (General) ,Rhodophyta - Abstract
Chemical investigation of the marine algal-derived endophytic fungus Aspergillus alabamensis EN-547 resulted in the isolation of 4-epi-seco-shornephine A methyl ester (1) and 4-epi-seco-shornephine A carboxylic acid (2), two new secondary metabolites having a rare diketomorpholine motif, and 28-acetoxy-12β,15α,25-trihydroxyergosta-4,6,8(14),22-tetraen-3-one (3), a new highly conjugated ergostane-type steroid, together with four known metabolites (4–7). Their chemical structures were elucidated by detailed analysis of their NMR spectra, ECDs, HRESIMS, optical rotation, and X-ray crystallographic data, and by comparison with literature data as well. The antimicrobial activities of compounds 1–7 were evaluated.
- Published
- 2018
109. Simpterpenoid A, a Meroterpenoid with a Highly Functionalized Cyclohexadiene Moiety Featuring gem-Propane-1,2-dione and Methylformate Groups, from the Mangrove-Derived Penicillium simplicissimum MA-332
- Author
-
Sui-Qun Yang, Rui Xu, Bin-Gui Wang, Xiao-Ming Li, Ling-Hong Meng, and Hong-Lei Li
- Subjects
Steric effects ,biology ,010405 organic chemistry ,Stereochemistry ,Organic Chemistry ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,chemistry.chemical_compound ,Penicillium simplicissimum ,chemistry ,Propane ,biology.protein ,Moiety ,Physical and Theoretical Chemistry ,Neuraminidase - Abstract
Simpterpenoid A (1), an unprecedented meroterpenoid possessing a highly functionalized cyclohexadiene moiety (ring C) featuring gem-propane-1,2-dione and methylformate groups, was characterized from mangrove-derived Penicillium simplicissimum MA-332. Due to the highly oxygenated and functionalized ring C and lack of some key correlations, the unambiguous assignment of the planar structure and steric configuration was solved by X-ray crystallographic analysis. Compound 1 exhibited inhibitory activity against influenza neuraminidase in nanomolar quantities.
- Published
- 2018
110. Polyketides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioides
- Author
-
Zhang, Fan-Zhong, primary, Li, Xiao-Ming, additional, Li, Xin, additional, Yang, Sui-Qun, additional, Meng, Ling-Hong, additional, and Wang, Bin-Gui, additional
- Published
- 2019
- Full Text
- View/download PDF
111. Thiocladospolides A–D, 12-Membered Macrolides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioides MA-299 and Structure Revision of Pandangolide 3
- Author
-
Zhang, Fan-Zhong, primary, Li, Xiao-Ming, additional, Yang, Sui-Qun, additional, Meng, Ling-Hong, additional, and Wang, Bin-Gui, additional
- Published
- 2019
- Full Text
- View/download PDF
112. Three New Indole Diterpenoids from the Sea-Anemone-Derived Fungus Penicillium sp. AS-79
- Author
-
Xin Li, Sui-Qun Yang, Xue-Yi Hu, Bin-Gui Wang, Ling-Hong Meng, and Xiaoming Li
- Subjects
Models, Molecular ,Indoles ,Stereochemistry ,Pharmaceutical Science ,Fungus ,Biology ,Sea anemone ,Penicillium sp ,01 natural sciences ,Article ,X-Ray Diffraction ,Drug Discovery ,Botany ,Animals ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Indole test ,endophytic fungus ,antimicrobial activity ,Molecular Structure ,secondary metabolites ,010405 organic chemistry ,Penicillium ,Endophytic fungus ,Anthozoa ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Diterpenes - Abstract
Three new indolediterpenoids, namely, 22-hydroxylshearinine F (1), 6-hydroxylpaspalinine (2), and 7-O-acetylemindole SB (3), along with eight related known analogs (4–11), were isolated from the sea-anemone-derived fungus Penicillium sp. AS-79. The structures and relative configurations of these compounds were determined by a detailed interpretation of the spectroscopic data, and their absolute configurations were determined by ECD calculations (1 and 2) and single-crystal X-ray diffraction (3). Some of these compounds exhibited prominent activity against aquatic and human pathogenic microbes.
- Published
- 2017
- Full Text
- View/download PDF
113. [Coexpression of MAP2K4 and vimentin proteins in human endometrial carcinoma and its clinicopathological significance]
- Author
-
Chun-Hua, Liu, Qing-Ping, Jiang, Dan, Lin, Wei, Chen, Yan-Yi, Xiao, Li, Lin, Yuan-Run, Deng, Hui-Ping, Jiang, and Sui-Qun, Guo
- Subjects
Survival Rate ,临床研究 ,MAP Kinase Kinase 4 ,Humans ,Vimentin ,Female ,Prognosis ,Endometrial Neoplasms - Abstract
OBJECTIVE: To analyze the expression of MAP2K4 and vimentin in human endometrial carcinoma (EC) and their association with the clinicopathological features and prognosis of the patients. METHODS: MAP2K4 and vimentin expressions were detected immunohistochemically in paraffin-embedded tissue sections from 128 patients with EC, and the correlation of MAP2K4 and vimentin expressions with the clinicopathological factors of the patients was analyzed. RESULTS: MAP2K4 and vimentin proteins were positively expressed in 49 (38.3%) and 83 (64.8%) of the patients, respectively. A positive expression of MAP2K4 was negatively correlated with FIGO stage of the tumor (P=0.010) and lymph node status (P=0.016); a positive expression of vimentin was positively correlated with FIGO stage of the tumor (P=0.025), histological grades (P=0.017), depth of myometrial invasion (P=0.044) and lymph node status (P=0.032). MAP2K4 was inversely associated with vimentin expression in EC(r=-0.598, P<0.001). Patients positive for MAP2K4 tended to have a higher overall survival rate (P=0.002), and those positive for vimentin tended to have a lower overall survival rate (P=0.007); patients positive for MAP2K4 but negative for vimentin had the longest survival time, while those negative for MAP2K4 and positive for vimentin had lowest survival rate (P=0.004). CONCLUSION: Detection of MAP2K4 and vimentin might help in early diagnosis and prognostic evaluation of patients with EC.
- Published
- 2017
114. High Nuclear Expression of HDGF Correlates with Disease Progression and Poor Prognosis in Human Endometrial Carcinoma
- Author
-
Sui-Qun Guo, Qiaofen Fu, Mengyang Zhao, Weiyi Fang, Shengni Hua, Qiangyun Wu, Qingping Jiang, and Lijing Wang
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Article Subject ,Clinical Biochemistry ,Gene Expression ,Kaplan-Meier Estimate ,Biology ,Endometrium ,Gene expression ,Biomarkers, Tumor ,Genetics ,Carcinoma ,medicine ,Humans ,Molecular Biology ,Grading (tumors) ,Lymph node ,Aged ,Proportional Hazards Models ,Aged, 80 and over ,Cell Nucleus ,lcsh:R5-920 ,Proportional hazards model ,Biochemistry (medical) ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Endometrial Neoplasms ,Cell nucleus ,medicine.anatomical_structure ,Lymphatic Metastasis ,Disease Progression ,Cancer research ,Intercellular Signaling Peptides and Proteins ,Immunohistochemistry ,Female ,lcsh:Medicine (General) ,Research Article - Abstract
Aims. This study examined the correlation between high nuclear expression of hepatoma-derived growth factor (HDGF) and clinicopathologic data in endometrial carcinoma (EC), including patient survival.Methods. One hundred and twenty-two endometrial carcinoma (EC) patients from 2002 to 2008 were reviewed in the study. HDGF expression in tumor tissues was examined using immunohistochemistry (IHC), and its association with clinicopathological parameters was evaluated. Tumors with 80% or more nuclei staining were regarded as high expression and tumors with less than 80% nuclei staining considered as low expression.Results and Conclusions. Immunohistochemical analysis revealed that HDGF was expressed in both the nucleus and cytoplasm. High nuclear expression of HDGF was positively correlated with FIGO stage (P=0.032), but not associated with other clinical features, such as histological grading or lymph node status. Patients with high expression of HDGF had poorer overall survival rates than those with low expression of HDGF (P=0.001). However, multivariate analyses showed that high nuclear expression of HDGF protein was not an independent predictor of prognosis for EC patients (P=0.111). Our results suggest that high nuclear expression of HDGF is a potential unfavorable factor for the progression and prognosis of EC.
- Published
- 2014
115. [Role of specificity protein 1 in modulating radiosensitivity of cervical cancer cell lines]
- Author
-
Yuan-Run, Deng, Hui-Ping, Jiang, Lan-Fang, Wu, Wei, Chen, Dan, Lin, and Sui-Qun, Guo
- Subjects
Sp1 Transcription Factor ,Cell Line, Tumor ,Gene Knockdown Techniques ,Humans ,Uterine Cervical Neoplasms ,Female ,RNA, Small Interfering ,Radiation Tolerance ,HeLa Cells - Abstract
To investigate the role of specificity protein 1 (Sp1) in regulating radiosensitivity of cervical cancer cell lines.We analyzed Sp1 expression in 6 different cervical cancer cell lines (SiHa, HeLa, Caski, Me180, Ms751, and C33a) using Western blotting and real-time PCR. Clonogenic survival assay and curve fitting were used to assess the changes in radiosensitivity of Me180 cells transfected with lentivirus-mediated shRNA vector targeting sp1 and HeLa cells transfected with sp1 over-expression vector.In the 6 cell lines tested, the cellular expression levels of Sp1 decreased gradually in the order of Me180, Caski, C33a, SiHa, Ms751, and HeLa. SP1 knockdown with lentivirus-mediated shRNA significantly lowered the survival rate of Me180 cells following radiation exposure (P0.05), and obviously lowered the values of SF2, D0 and Dq but significantly increased α/β of the cells. Compared with the cells transfected with the mock vector, HeLa cells with sp1 over-expression showed a significantly increased survival following radiation exposure (P0.05) with obviously increased values of SF2, D0 and Dq but significantly lowered α/β.Silencing Sp1 can increase the radiosensitivity while Sp1 overexpression enhances the radioresistance of cervical cancer cell lines, suggesting an important role of Sp1 in radiotherapy for cervical cancer.
- Published
- 2016
116. Polyketides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioides
- Author
-
Sui-Qun Yang, Bin-Gui Wang, Fan-Zhong Zhang, Ling-Hong Meng, Xin Li, and Xiaoming Li
- Subjects
Stereochemistry ,Pharmaceutical Science ,Cladosporium cladosporioides ,Bruguiera ,01 natural sciences ,Drug Discovery ,lcsh:QH301-705.5 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,IC50 ,chemistry.chemical_classification ,endophytic fungus ,antimicrobial activity ,biology ,010405 organic chemistry ,acetylcholinesterase ,Endophytic fungus ,biology.organism_classification ,Antimicrobial ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Enzyme ,lcsh:Biology (General) ,chemistry ,enzymatic inhibitory activity ,mangrove plant ,Mangrove ,Two-dimensional nuclear magnetic resonance spectroscopy ,polyketides - Abstract
Five new polyketides, namely, 5R-hydroxyrecifeiolide (1), 5S-hydroxyrecifeiolide (2), ent-cladospolide F (3), cladospolide G (4), and cladospolide H (5), along with two known compounds (6 and 7), were isolated from the endophytic fungal strain Cladosporium cladosporioides MA-299 that was obtained from the leaves of the mangrove plant Bruguiera gymnorrhiza. The structures of these compounds were established by extensive analysis of 1D/2D NMR data, mass spectrometric data, ECDs and optical rotations, and modified Mosher&rsquo, s method. The structures of 3 and 6 were confirmed by single-crystal X-ray diffraction analysis and this is the first time for reporting the crystal structures of these two compounds. All of the isolated compounds were examined for antimicrobial activities against human and aquatic bacteria and plant pathogenic fungi as well as enzymatic inhibitory activities against acetylcholinesterase. Compounds 1&ndash, 4, 6, and 7 exhibited antimicrobial activity against some of the tested strains with MIC values ranging from 1.0 to 64 &mu, g/mL, while 3 exhibited enzymatic inhibitory activity against acetylcholinesterase with the IC50 value of 40.26 &mu, M.
- Published
- 2019
117. Simpterpenoid A, a Meroterpenoid with a Highly Functionalized Cyclohexadiene Moiety Featuring gem-Propane-1,2-dione and Methylformate Groups, from the Mangrove-Derived Penicillium simplicissimumMA-332
- Author
-
Li, Hong-Lei, Xu, Rui, Li, Xiao-Ming, Yang, Sui-Qun, Meng, Ling-Hong, and Wang, Bin-Gui
- Abstract
Simpterpenoid A (1), an unprecedented meroterpenoid possessing a highly functionalized cyclohexadiene moiety (ring C) featuring gem-propane-1,2-dione and methylformate groups, was characterized from mangrove-derived Penicillium simplicissimumMA-332. Due to the highly oxygenated and functionalized ring C and lack of some key correlations, the unambiguous assignment of the planar structure and steric configuration was solved by X-ray crystallographic analysis. Compound 1exhibited inhibitory activity against influenza neuraminidase in nanomolar quantities.
- Published
- 2024
- Full Text
- View/download PDF
118. Two New Diketomorpholine Derivatives and a New Highly Conjugated Ergostane-Type Steroid from the Marine Algal-Derived Endophytic Fungus Aspergillus alabamensis EN-547
- Author
-
Yang, Sui-Qun, primary, Li, Xiao-Ming, additional, Li, Xin, additional, Chi, Lu-Ping, additional, and Wang, Bin-Gui, additional
- Published
- 2018
- Full Text
- View/download PDF
119. Simpterpenoid A, a Meroterpenoid with a Highly Functionalized Cyclohexadiene Moiety Featuring gem-Propane-1,2-dione and Methylformate Groups, from the Mangrove-Derived Penicillium simplicissimum MA-332
- Author
-
Li, Hong-Lei, primary, Xu, Rui, additional, Li, Xiao-Ming, additional, Yang, Sui-Qun, additional, Meng, Ling-Hong, additional, and Wang, Bin-Gui, additional
- Published
- 2018
- Full Text
- View/download PDF
120. Isolation, Synthesis, and Radical-Scavenging Activity of Rhodomelin A, a Ureidobromophenol from the Marine Red Alga Rhodomela confervoides
- Author
-
Li, Ke, primary, Wang, Ya-Fei, additional, Li, Xiao-Ming, additional, Wang, Wei-Jia, additional, Ai, Xu-Ze, additional, Li, Xin, additional, Yang, Sui-Qun, additional, Gloer, James B., additional, Wang, Bin-Gui, additional, and Xu, Tao, additional
- Published
- 2017
- Full Text
- View/download PDF
121. [Expressions of DAPK3 and c-Myc in endometrial cancer and their relationship with the patients' prognosis]
- Author
-
Chen, Chen and Sui-Qun, Guo
- Subjects
Proto-Oncogene Proteins c-myc ,Survival Rate ,Death-Associated Protein Kinases ,Lymphatic Metastasis ,Humans ,Female ,Kaplan-Meier Estimate ,Prognosis ,Endometrial Neoplasms - Abstract
To investigate the expressions of DAPK3 and c-Myc and their prognostic value in endometrial carcinoma (EC).The expressions of DAPK3 and c-Myc were detected immunohistochemically in 132 surgical specimens. The relationship between DAPK3 and c-Myc protein expressions and the clinicopathological features of the patients was evaluated.Immunohistochemical analysis revealed low DAPK3 expression in 60.61% (80/132) and high c-Myc expression in 53.79% (71/132) of the specimens of EC. Both DAPK3 expression and c-Myc expression were significantly correlated with FIGO stage (P=0.034 and 0.015, respectively) and lymph node metastasis (P=0.022 and 0.031, respectively). DAPK3 expression was closely correlated with the histological grade (P=0.027) and depth of myometrial invasion (P=0.011). Kaplan-Meier survival analysis indicated that patients with low DAPK3 expressions had a shorter overall survival rate than those with high DAPK3 expressions (P=0.023), while patients with high c-Myc expressions had poorer prognoses than those with low c-Myc expressions (P=0.002). Spearman correlation analysis showed that DAPK3 and c-Myc expressions were negatively correlated (P0.001, r=?0.310). Multivariate analysis identified a high c-Myc expression as the independent predictor of the prognosis of EC (P=0.007).A low expression of DAPK3 and a high expression of c-Myc are associated with aggressive and metastatic behaviors of EC, and their detection may help to predict the prognosis of the EC patients.
- Published
- 2016
122. Three New Indole Diterpenoids from the Sea-Anemone-Derived Fungus Penicillium sp. AS-79
- Author
-
Hu, Xue-Yi, primary, Meng, Ling-Hong, additional, Li, Xin, additional, Yang, Sui-Qun, additional, Li, Xiao-Ming, additional, and Wang, Bin-Gui, additional
- Published
- 2017
- Full Text
- View/download PDF
123. Stereochemical Elucidation of Natural Products from Residual Chemical Shift Anisotropies in a Liquid Crystalline Phase
- Author
-
Li, Xiao-Lu, Chi, Lu-Ping, Navarro-Vázquez, Armando, Hwang, Songhwan, Schmieder, Peter, Li, Xiao-Ming, Li, Xin, Yang, Sui-Qun, Lei, Xinxiang, Wang, Bin-Gui, and Sun, Han
- Abstract
Determination of the stereochemistry of organic molecules still represents one of the major obstacles in the structure elucidation procedure in drug discovery. Although the application of residual dipolar couplings (RDCs) has revolutionized this field, residual chemical shift anisotropies (RCSAs) which contain valuable structural information for nonprotonated carbons have only been scarcely employed so far. In this study, we present a simple but highly effective solution to extract RCSAs of the analytes in a liquid crystalline phase formed by AAKLVFF oligopeptides. This method does not require any special instruments, devices, or correction during postacquisition data analysis and thus can be easily applied in any chemistry laboratory. To illustrate the potential of this method, the relative configurations of four known natural products (1–4) belonging to different structural classes were confirmed. Moreover, we unambiguously elucidated the stereochemistry of spiroepicoccin A (5), a rare thiodiketopiperazine marine natural product whose configuration could not be assigned based on conventional NMR methods.
- Published
- 2020
- Full Text
- View/download PDF
124. Two new terpenoid glucosides from Aster flaccidus
- Author
-
Jian Min Yue, Li-She Gan, Zha-Jun Zhan, and Sui-Qun Yang
- Subjects
China ,Herb medicine ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Monoterpene ,Aster Plant ,Pharmaceutical Science ,Pharmacognosy ,Sesquiterpene ,Aster flaccidus ,Analytical Chemistry ,chemistry.chemical_compound ,Glucosides ,Glucoside ,Drug Discovery ,Botany ,Pharmacology ,chemistry.chemical_classification ,Plants, Medicinal ,Molecular Structure ,Terpenes ,Organic Chemistry ,Glycoside ,General Medicine ,Plant Components, Aerial ,Terpenoid ,Complementary and alternative medicine ,chemistry ,Monoterpenes ,Molecular Medicine ,Sesquiterpenes - Abstract
Two new terpenoid glucosides, namely 2-O-beta-D-glucopyranoside-vicodiol (1) and 10-O-beta-D-glucopyranoside-oplopanone (2), along with seven known compounds, were isolated from the aerial part of Aster flaccidus (composite), a traditional Chinese herb medicine. The structures of 1 and 2 were established by spectroscopic methods, especially 2D NMR experiments.
- Published
- 2006
125. Enolase-1 is a therapeutic target in endometrial carcinoma
- Author
-
Yan Wang, Zhen Liu, Qiaofen Fu, Yiyu Chen, Yan Liu, Luyun Shu, Qinping Jiang, Yiyi Liu, Lijing Wang, Rongcheng Luo, Weiyi Fang, Xiaojie Deng, Sui-Qun Guo, Zhong Mei, Shengni Hua, Mengyang Zhao, and Yue Fan
- Subjects
Male ,ENO1 ,medicine.disease_cause ,Metastasis ,Endometrium ,Mice ,Cell Movement ,cell growth ,RNA, Small Interfering ,Mice, Inbred BALB C ,Traditional medicine ,EMT ,glycolysis ,Class Ia Phosphatidylinositol 3-Kinase ,DNA-Binding Proteins ,Gene Expression Regulation, Neoplastic ,Cell Transformation, Neoplastic ,Oncology ,Female ,RNA Interference ,Signal Transduction ,Research Paper ,Epithelial-Mesenchymal Transition ,Transplantation, Heterologous ,Mice, Nude ,endometrial carcinoma ,Cell Line, Tumor ,Carcinoma ,medicine ,Biomarkers, Tumor ,Animals ,Humans ,Neoplasm Invasiveness ,Epithelial–mesenchymal transition ,RNA, Messenger ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,business.industry ,Cell growth ,Tumor Suppressor Proteins ,Cancer ,medicine.disease ,Endometrial Neoplasms ,Phosphopyruvate Hydratase ,Cancer research ,Cisplatin ,business ,Carcinogenesis ,Proto-Oncogene Proteins c-akt ,Neoplasm Transplantation - Abstract
// Mengyang Zhao 1, 2, * , Weiyi Fang 1, 2, * , Yan Wang 2, 5, * , Suiqun Guo 6, * , Luyun Shu 6 , Lijing Wang 2, 6 , YiYu Chen 2 , Qiaofen Fu 2 , Yan Liu 2 , Shengni Hua 2 , Yue Fan 2 , Yiyi Liu 2 , Xiaojie Deng 2 , Rongcheng Luo 1 , Zhong Mei 5 , Qinping Jiang 2, 4 , Zhen Liu 2, 3 1 Cancer Center, Traditional Chinese Medicine-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong, China 2 Cancer Research Institute, Southern Medical University, Guangzhou, China 3 Department of Pathology of Basic School, Medical University of Guangzhou, Guangzhou, China 4 Department of Pathology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, China 5 Department of Obstetrics and Gynecology of Nanfang Hospital, Southern Medical University, Guangzhou, China 6 Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, China * These authors have contributed equally to this work Correspondence to: Weiyi Fang, e-mail: fangweiyi1975@163.com Zhen Liu, e-mail: narcissus_jane@163.com Qingping Jiang, e-mail: 413430005@qq.com Keywords: ENO1, endometrial carcinoma, cell growth, EMT, glycolysis Received: December 30, 2014 Accepted: April 10, 2015 Published: April 24, 2015 ABSTRACT ENO1 plays a paradoxical role in driving the pathogenesis of tumors. However, the clinical significance of ENO1 expression remains unclear and its function and modulatory mechanisms have never been reported in endometrial carcinoma (EC). In this study, ENO1 silencing significantly reduced cell glycolysis, proliferation, migration, and invasion in vitro , as well as tumorigenesis and metastasis in vivo by modulating p85 suppression. This in turn mediated inactivation of PI3K/AKT signaling and its downstream signals including glycolysis, cell cycle progression, and epithelial-mesenchymal transition (EMT)-associated genes. These effects on glycolysis and cell growth were not observed after ENO1 suppression in normal human endometrial epithelial cells (HEEC). Knocking down ENO1 could significantly enhance the sensitivity of EC cells to cisplatin (DDP) and markedly inhibited the growth of EC xenografts in vivo . In clinical samples, EC tissues exhibited higher expression levels of ENO1 mRNA and protein compared with normal endometrium tissues. Patients with higher ENO1 expression had a markedly shorter overall survival than patients with low ENO1 expression. We conclude that ENO1 favors carcinogenesis, representing a potential target for gene-based therapy.
- Published
- 2014
126. Subsampling and Active Learning.
- Author
-
Sui, Qun
- Published
- 2023
127. Simpterpenoid A, a Meroterpenoid with a Highly Functionalized Cyclohexadiene Moiety Featuring gem-Propane-1,2-dione and Methylformate Groups, from the Mangrove-Derived Penicillium simplicissimum MA-332.
- Author
-
Hong-Lei Li, Rui Xu, Xiao-Ming Li, Sui-Qun Yang, Ling-Hong Meng, and Bin-Gui Wang
- Published
- 2018
- Full Text
- View/download PDF
128. Isolation, Synthesis, and Radical-Scavenging Activity of Rhodomelin A, a Ureidobromophenol from the Marine Red Alga Rhodomela confervoides.
- Author
-
Ke Li, Ya-Fei Wang, Xiao-Ming Li, Wei-Jia Wang, Xu-Ze Ai, Xin Li, Sui-Qun Yang, Gloer, James B., Bin-Gui Wang, and Tao Xu
- Published
- 2018
- Full Text
- View/download PDF
129. Thiocladospolides A–D, 12-Membered Macrolides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioidesMA-299 and Structure Revision of Pandangolide 3
- Author
-
Zhang, Fan-Zhong, Li, Xiao-Ming, Yang, Sui-Qun, Meng, Ling-Hong, and Wang, Bin-Gui
- Abstract
Four new 12-membered macrolides, thiocladospolides A–D (1–4), each possessing a sulfur substitution at C-2, along with the known congener pandangolide 3 (5) and the possible hydrolysis product seco-patulolide C (6), were isolated and identified from the culture extract of Cladosporium cladosporioidesMA-299, an endophytic fungus obtained from the leaves of the mangrove plant Bruguiera gymnorrhiza. Their structures were established by interpretation of the NMR spectroscopic and mass spectrometric data. X-ray crystallographic analysis of compound 1, which represents the first crystal structure described for a sulfur-containing 12-membered macrolide, confirmed its structure and absolute configuration. The proposed sulfur side chain at C-3 in pandangolide 3 (5) was revised to be at C-2 by detailed analysis of the NMR data and by a comparison with data for thiocladospolide A (1). The structures for pandangolides 2 and 4 should also be considered for revisions. The isolated compounds were evaluated for the antimicrobial activities against aquatic bacteria and plant pathogens.
- Published
- 2019
- Full Text
- View/download PDF
130. [Identification and genotyping of oncogenic type of human papillomavirus in paraffin-embedded cervical cancer samples in Guangzhou]
- Author
-
Li, Zeng, Shou-yi, Yu, Sui-qun, Gou, Jin-ping, Yun, and Jun, Zhang
- Subjects
China ,Human papillomavirus 16 ,Genotype ,Human papillomavirus 18 ,DNA, Viral ,Papillomavirus Infections ,Humans ,Uterine Cervical Neoplasms ,Female ,Papillomaviridae ,Polymerase Chain Reaction - Abstract
To investigate human papillomavirus (HPV) infection and genotyping in patients with cervical cancer in Guangzhou in the last 3 decades.HPV L1 gene fragment in paraffin-embedded cervical cancer samples was amplified by HPV-specific PCR with consensus primers, and typing of HPV strains was performed on the basis of sequence analysis of the PCR products.The positivity rates of HPV DNA was 26.2% in the 99 cases of cervical cancer. Five HPV genotypes were identified including HPV16, 18, 33, 52 and 58.HPV16, 58 and 33 are the most common genotypes of HPV, accounting for over 88.4% in the total infected cases, suggesting that the HPV genotypes closely related to cervical cancer is more centralized in Guangzhou.
- Published
- 2009
131. [Event-related potentials of emotional excitement in middle-aged and aged adults]
- Author
-
Hong-zhen, Zhou, Sui-qun, Guo, Yi-feng, Luo, Ling-li, Chen, and Shu, Zhou
- Subjects
Adult ,Male ,Time Factors ,Health ,Emotions ,Intelligence ,Evoked Potentials, Visual ,Humans ,Female ,Middle Aged ,Aged - Abstract
To explore how adjustment of the negative and neutral emotion excitement contribute to the well-being of middle-aged and aged adults in their health, intelligence, working and living.Twenty-five middle-aged and aged participants performed recognition tasks of schematic facial representations of positive, neutral and negative emotions and also Chinese characters (equivalent to happy, thinking and fear).The main effects of the facial recognition task occurred in the left temporal-parietal area (40 to 80 ms), bilateral parietal-occipital and temporal-parietal area (160-180 ms and 310-340 ms). The effects of stimulation with the 3 Chinese characters were found in the anterior region (375-475 ms), posterior region (195-255 ms), and whole scalp region (135-175 ms and 275-355 ms). There were no significant interactive effects between schematic facial recognition and stimulation with Chinese characters.Positive emotional excitement may promote the well-being of the adults in their health, intelligence, working performance and living.
- Published
- 2007
132. [Preliminary study of gene expression profiling in human type I and II endometrial carcinoma]
- Author
-
Sui-qun, Guo, Fu-qi, Xing, Zhan-jun, Pang, Wei-yi, Fang, and Guo-bing, Liu
- Subjects
Gene Expression Regulation, Neoplastic ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,Humans ,Female ,Adenocarcinoma ,Microarray Analysis ,Adenocarcinoma, Clear Cell ,Endometrial Neoplasms - Abstract
To study gene expression profiling in human type I and II endometrial carcinoma.Six Affymetrix human genome genechips were utilized to investigate the differences in gene expression profiles between type I and II endometrial carcinoma with bioinformatic analysis.Many genes were highly expressed in estrogen-dependent endometrial carcinoma, and some of them were involved in the metabolism and conversion of estrogen, while some others in estrogen regulation. CYP2C9, for instance, was involved in the conversion of estrogen sulfate to 16-hydroxy sulfate metabolite, DDC in estrogen-dependent pathogenesis of endometrial carcinoma possibly by DDC interaction with AR to enhance steroid receptor transcription.High expression of these genes in estrogen-dependent endometrial carcinoma may provide insights into their roles in the pathogenesis and prognosis of this malignancy.
- Published
- 2006
133. Three New Indole Diterpenoids from the Sea-Anemone-Derived Fungus Penicillium sp. AS-79.
- Author
-
Xue-Yi Hu, Ling-Hong Meng, Xin Li, Sui-Qun Yang, Xiao-Ming Li, and Bin-Gui Wang
- Abstract
Three new indolediterpenoids, namely, 22-hydroxylshearinine F (1), 6-hydroxylpaspalinine (2), and 7-O-acetylemindole SB (3), along with eight related known analogs (4-11), were isolated from the sea-anemone-derived fungus Penicillium sp. AS-79. The structures and relative configurations of these compounds were determined by a detailed interpretation of the spectroscopic data, and their absolute configurations were determined by ECD calculations (1 and 2) and single-crystal X-ray diffraction (3). Some of these compounds exhibited prominent activity against aquatic and human pathogenic microbes. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
134. [Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing]
- Author
-
Xin, Liu, Guo-Bing, Liu, Zhan-Jun, Pang, Fu-Qi, Xing, Sui-Qun, Guo, Wen-Li, Ma, and Wen-Ling, Zheng
- Subjects
Human papillomavirus 16 ,Human papillomavirus 18 ,DNA, Viral ,Papillomavirus Infections ,Carcinoma, Squamous Cell ,Humans ,Uterine Cervical Neoplasms ,Female ,Adenocarcinoma ,Polymerase Chain Reaction - Abstract
To explore an effective method for detecting human papillomavirus (HPV) DNA in cervical cancer tissue.HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product.The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections. Infection with HPV58 was detected in one case. The sequencing results showed no difference in L1 sequence between the detected samples and the standard German HPV58 strain.PCR and direct sequencing approach is effective for detecting and typing of HPV DNA in cervical cancer tissue, through which rare HPV strain or mutants of known HPV strains may not escape detection.
- Published
- 2005
135. Prenylated Phenol and Benzofuran Derivatives from Aspergillus terreus EN-539, an Endophytic Fungus Derived from Marine Red Alga Laurencia okamurai.
- Author
-
Li, Hong-Lei, Li, Xiao-Ming, Yang, Sui-Qun, Meng, Ling-Hong, Li, Xin, and Wang, Bin-Gui
- Abstract
Three new prenylated phenol derivatives, terreprenphenols A–C (1–3), along with four known related compounds (4–7), were isolated from Aspergillus terreus EN-539, an endophytic fungus obtained from the marine red alga Laurencia okamurai. The structures of these compounds were established by extensive analysis of 1D/2D NMR data, mass spectrometric data, and optical rotation (OR). The corresponding relationship between absolute configuration and optical rotation for known compounds anodendroic acid (4) and asperterreusine C (5) was ambiguous in literature, and their absolute configurations were therefore discussed and confirmed for the first time by time-dependent density functional (TDDFT) ECD and OR calculations. Compounds 1–7 inhibited some common aquatic bacteria with MIC values ranging from 2 to 64 μg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
136. Cytochalasin Derivatives from the Endozoic Curvularia verruculosaCS-129, a Fungus Isolated from the Deep-Sea Squat Lobster Shinkaia crosnieriLiving in the Cold Seep Environment
- Author
-
Hu, Xue-Yi, Wang, Chen-Yin, Li, Xiao-Ming, Yang, Sui-Qun, Li, Xin, Wang, Bin-Gui, Si, Shu-Yi, and Meng, Ling-Hong
- Abstract
A new cytochalasin dimer, verruculoid A (1), three new cytochalasin derivatives, including 12-nor-cytochalasin F (2), 22-methoxycytochalasin B6(3), and 19-hydroxycytochalasin B (4), and 20-deoxycytochalasin B (5), a synthetic product obtained as a natural product for the first time, together with four known analogues (6–9), were isolated and identified from the culture extract of Curvularia verruculosaCS-129, an endozoic fungus obtained from the inner fresh tissue of the deep-sea squat lobster Shinkaia crosnieri, which was collected from the cold seep area of the South China Sea. Structurally, verruculoid A (1) represents the first cytochalasin homodimer containing a thioether bridge, while 12-nor-cytochalasin F (2) is the first 12-nor-cytochalasin derivative. Their structures were elucidated by detailed interpretation of the NMR spectroscopic and mass spectrometric data. X-ray crystallographic analysis and ECD calculations confirmed their structures and absolute configurations. Compound 1displayed activity against the human pathogenic bacterium Escherichia coli(MIC = 2 μg/mL), while compounds 4, 8, and 9showed cytotoxicity against three tumor cell lines (HCT-116, HepG-2, and MCF-7) with IC50values from 5.2 to 12 μM. The structure–activity relationship was briefly discussed.
- Published
- 2021
- Full Text
- View/download PDF
137. Polyketides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioides.
- Author
-
Li, Xiao-Ming, Li, Xin, Yang, Sui-Qun, Meng, Ling-Hong, Zhang, Fan-Zhong, and Wang, Bin-Gui
- Abstract
Five new polyketides, namely, 5R-hydroxyrecifeiolide (1), 5S-hydroxyrecifeiolide (2), ent-cladospolide F (3), cladospolide G (4), and cladospolide H (5), along with two known compounds (6 and 7), were isolated from the endophytic fungal strain Cladosporium cladosporioides MA-299 that was obtained from the leaves of the mangrove plant Bruguiera gymnorrhiza. The structures of these compounds were established by extensive analysis of 1D/2D NMR data, mass spectrometric data, ECDs and optical rotations, and modified Mosher's method. The structures of 3 and 6 were confirmed by single-crystal X-ray diffraction analysis and this is the first time for reporting the crystal structures of these two compounds. All of the isolated compounds were examined for antimicrobial activities against human and aquatic bacteria and plant pathogenic fungi as well as enzymatic inhibitory activities against acetylcholinesterase. Compounds 1–4, 6, and 7 exhibited antimicrobial activity against some of the tested strains with MIC values ranging from 1.0 to 64 μg/mL, while 3 exhibited enzymatic inhibitory activity against acetylcholinesterase with the IC
50 value of 40.26 μM. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
138. Two New Diketomorpholine Derivatives and a New Highly Conjugated Ergostane-Type Steroid from the Marine Algal-Derived Endophytic Fungus Aspergillusalabamensis EN-547.
- Author
-
Li, Xiao-Ming, Li, Xin, Wang, Bin-Gui, Yang, Sui-Qun, and Chi, Lu-Ping
- Abstract
Chemical investigation of the marine algal-derived endophytic fungus
Aspergillus alabamensis EN-547 resulted in the isolation of 4-epi -seco -shornephine A methyl ester (1 ) and 4-epi -seco -shornephine A carboxylic acid (2 ), two new secondary metabolites having a rare diketomorpholine motif, and 28-acetoxy-12β ,15α ,25-trihydroxyergosta-4,6,8(14),22-tetraen-3-one (3 ), a new highly conjugated ergostane-type steroid, together with four known metabolites (4 –7 ). Their chemical structures were elucidated by detailed analysis of their NMR spectra, ECDs, HRESIMS, optical rotation, and X-ray crystallographic data, and by comparison with literature data as well. The antimicrobial activities of compounds1 –7 were evaluated. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
139. From rainforest to herbland: New insights into land plant responses to the end-Permian mass extinction.
- Author
-
Feng, Zhuo, Wei, Hai-Bo, Guo, Yun, He, Xiao-Yuan, Sui, Qun, Zhou, Yu, Liu, Hang-Yu, Gou, Xu-Dong, and Lv, Yong
- Subjects
- *
MASS extinctions , *VEGETATION dynamics , *MARINE animals , *BIOLOGICAL extinction , *PLANTS - Abstract
The end-Permian mass extinction is the greatest biotic crisis in Earth history causing the extinction of a large number of marine and terrestrial animals globally. However, how land plants responded to the catastrophe remains controversial. The successive plant-bearing beds in China provide a unique window into the great vegetation change through the critical Permian–Triassic time interval. A notable monospecific vegetation comprising a new isoetalean, Tomiostrobus sinensis Feng, is documented from the lowermost part of the coastal Kayitou Formation in Southwest China. The new plant is a diminutive, heterosporous lycophyte, which formed ground-covering communities infringing the oligotrophic lakes along coastal regions. The apparent occurrence of T. sinensis immediately above the uppermost coal bed demonstrates that the enigmatic gigantopterid-dominated rainforest ecosystem suddenly collapsed and was replaced by the isoetalean-dominated herbaceous vegetation in the tropics of the eastern Tethys. Our study indicates that the magnitude of vegetation response to the end-Permian mass extinction in different palaeophytogeographic regions is probably largely affected by the latitudinal gradients of biodiversity and ecological stress. Because of the complexity of local palaeoenvironmental, palaeoclimatic and palaeotopographic conditions, it is unlikely that a unified global pattern and timing of land plant response to the end-Permian mass extinction can be obtained. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
140. Three new α -pyrone derivatives from the soil-derived fungus Penicillium herquei MA-370.
- Author
-
Yang SQ, Li XM, Chen XD, Li X, and Wang BG
- Subjects
- Molecular Structure, Crystallography, X-Ray, Microbial Sensitivity Tests, Rhizophoraceae microbiology, Magnetic Resonance Spectroscopy, Penicillium chemistry, Pyrones chemistry, Pyrones pharmacology, Pyrones isolation & purification, Soil Microbiology
- Abstract
Three new α -pyrone derivatives, annularins L-N ( 1 - 3 ), were isolated from the EtOAc extract of Penicillium herquei MA-370, a fungus obtained from the rhizospheric soil of the mangrove plant Rhizophora mucronata. The planar structures of compounds 1 - 3 were determined based on comprehensive spectral interpretation of the NMR and MS data. The absolute configuration of 1 was determined by X-ray crystallographic data and that of 2 was assigned by TDDFT calculations of its ECD spectrum and cotton effects comparison with those of 1 . The antimicrobial activity of compounds 1 - 3 was evaluated.
- Published
- 2024
- Full Text
- View/download PDF
141. Discovery of (±)-Penindolenes Reveals an Unusual Indole Ring Cleavage Pathway Catalyzed by P450 Monooxygenase.
- Author
-
Meng LH, Awakawa T, Li XM, Quan Z, Yang SQ, Wang BG, and Abe I
- Subjects
- Biocatalysis, Stereoisomerism, Molecular Structure, Cytochrome P-450 Enzyme System metabolism, Indoles chemistry, Indoles metabolism, Penicillium enzymology, Penicillium metabolism
- Abstract
(±)-Penindolenes A-D (1-4), the first representatives of indole terpenoids featuring a γ-lactam skeleton, were isolated from the mangrove-derived endophytic fungus Penicillium brocae MA-231. Our bioactivity tests revealed their potent antimicrobial and acetylcholinesterase inhibitory activities. The biosynthetic reactions by the five enzymes PbaABCDE leading to γ-lactam ring formation were identified with heterologous expression and in vitro enzymatic assays. Remarkably, the cytochrome P450 monooxygenase PbaB and its homolog in Aspergillus oryzae catalyzed the 2,3-cleavage of the indole ring to generate two keto groups in 1. This is the first example of the oxidative cleavage of indole by a P450 monooxygenase. In addition, rare secondary amide bond formation by the glutamine synthetase-like enzyme PbaD was reported. These findings will contribute to the engineered biosynthesis of unnatural, bioactive indole terpenoids., (© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
142. New Hydroxyphenylacetic Acids and α-Pyrone Derivative from the Deep-Sea Cold Seep Sediment-Derived Fungus Penicillium corylophilum CS-682.
- Author
-
Zhu CS, Li XM, Yang SQ, Liu YW, Wang BG, and Li X
- Subjects
- Geologic Sediments microbiology, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Stereoisomerism, Phenylacetates chemistry, Phenylacetates isolation & purification, Phenylacetates pharmacology, Molecular Structure, Molecular Conformation, Penicillium chemistry, Pyrones chemistry, Pyrones pharmacology, Pyrones isolation & purification
- Abstract
Two pairs of new enantiomeric hydroxyphenylacetic acid derivatives, (±)-corylophenols A and B ((±)-1 and (±)-2), a new α-pyrone analogue, corylopyrone A (3), and six andrastin-type meroterpenoids (4-9) were isolated and identified from the deep-sea cold-seep sediment-derived fungus Penicillium corylophilum CS-682. Their structures and stereo configurations were determined by detailed spectroscopic analysis of NMR and MS data, chiral HPLC analysis, J-based configuration analysis, and quantum chemical calculations of ECD, specific rotation, and NMR (with DP4+ probability analysis). Compound 3 showed inhibitory activity against some strains of pathogenic bacteria., (© 2024 Wiley-VHCA AG, Zurich, Switzerland.)
- Published
- 2024
- Full Text
- View/download PDF
143. Antifungal Pseuboyenes A-J, Bergamotene-Derived Sesquiterpenoids from a Cold-Seep-Derived Pseudallescheria boydii .
- Author
-
Ying Z, Li XM, Yang SQ, Li HL, Li X, Wang BG, and Meng LH
- Subjects
- Molecular Structure, Colletotrichum drug effects, Fusarium drug effects, Crystallography, X-Ray, Antifungal Agents pharmacology, Antifungal Agents chemistry, Sesquiterpenes pharmacology, Sesquiterpenes chemistry, Sesquiterpenes isolation & purification, Microbial Sensitivity Tests, Pseudallescheria
- Abstract
A chemical investigation of a cold-seep-sediment-derived fungus, Pseudallescheria boydii CS-793, resulted in characterization of 10 novel bergamotene-derived sesquiterpenoids, pseuboyenes A-J ( 1 - 10 ). Their structures were elucidated by spectroscopic and X-ray crystallographic analyses as well as using the modified Mosher's method. Compound 1 represents the first example of a β -bergamotene containing a 6-oxobicyclo[3.2.1]octane nucleus adducted with a methyl lactate unit, while 8 - 10 involve a skeletal rearrangement from bergamotene. Compounds 2 - 5 showed significant antifungal activities against Colletotrichum gloeosporioides Penz. and Fusarium oxysporum with MICs ranging from 0.5 to 8 μ g/mL. Compound 4 exhibited an in vitro anti- F. proliferatum effect with an EC
50 value of 1.0 μ g/mL.- Published
- 2024
- Full Text
- View/download PDF
144. Antibacterial Polyketides from the Deep-Sea Cold-Seep-Derived Fungus Talaromyces sp. CS-258.
- Author
-
Wu Z, Li XM, Yang SQ, Wang BG, and Li X
- Subjects
- Microbial Sensitivity Tests, Molecular Structure, Talaromyces chemistry, Talaromyces metabolism, Polyketides pharmacology, Polyketides chemistry, Polyketides isolation & purification, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification
- Abstract
Thirty-two fungal polyketide derivatives, including eleven new compounds, namely (3 R ,5' R )-5-hydroxytalaroflavone ( 1 ), talaroisochromenols A-C ( 3 , 5 , and 11 ), (8 R ,9 R ,10a R )-5-hydroxyaltenuene ( 13 ), (8 R ,9 R ,10a S )-5-hydroxyaltenuene ( 14 ), (8 R ,9 S ,10a R )-5-hydroxyaltenuene ( 15 ), nemanecins D and E ( 25 and 26 ), 2,5-dimethyl-8-iodochromone ( 27 ), and talarofurolactone A ( 29 ), together with one new naturally occurring but previously synthesized metabolite, 6-hydroxy-4-methoxycoumarin ( 28 ), were isolated and identified from the deep-sea cold-seep-derived fungus Talaromyces sp. CS-258. Among them, racemic ((±)- 11 ) or epimeric ( 13 - 15 , 25 , and 26 ) mixtures were successfully separated by chiral or gradient elution HPLC. Meanwhile, compound 27 represents a rarely reported naturally occurring iodinated compound. Their planar structures as well as absolute configurations were determined by extensive analysis via NMR, MS, single-crystal X-ray diffraction, Mosher's method, and ECD or NMR calculation (with DP4
+ probability analysis). Possible biosynthetic routes of some isolated compounds, which are related to chromone or isochromone biosynthetic pathways, were put forward. The biological analysis results revealed that compounds 7 , 9, 10, 18 - 22 , 24 , 30 , and 31 showed broad-spectrum antibacterial activities against several human and aquatic pathogens with MIC ranges of 0.5-64 μg/mL.- Published
- 2024
- Full Text
- View/download PDF
145. Pseudallenes A and B, new sulfur-containing ovalicin sesquiterpenoid derivatives with antimicrobial activity from the deep-sea cold seep sediment-derived fungus Pseudallescheria boydii CS-793.
- Author
-
Ying Z, Li XM, Yang SQ, Li HL, Li X, Wang BG, and Meng LH
- Abstract
Pseudallenes A and B ( 1 and 2 ), the new and rare examples of sulfur-containing ovalicin derivatives, along with three known analogues 3 - 5 , were isolated and identified from the culture extract of Pseudallescheria boydii CS-793, a fungus obtained from the deep-sea cold seep sediments. Their structures were established by detailed interpretation of NMR spectroscopic and mass spectrometric data. X-ray crystallographic analysis confirmed and established the structures and absolute configurations of compounds 1 - 3 , thus providing the first characterized crystal structure of an ovalicin-type sesquiterpenoid. In the antimicrobial assays, compounds 1 - 3 showed broad-spectrum inhibitory activities against several plant pathogens with MIC values ranging from 2 to 16 μg/mL., (Copyright © 2024, Ying et al.)
- Published
- 2024
- Full Text
- View/download PDF
146. Bialorastins A-F, highly oxygenated and polycyclic andrastin-type meroterpenoids with proangiogenic activity from the deep-sea cold-seep-derived fungus Penicillium bialowiezense CS-283.
- Author
-
Yan LH, Li PH, Li XM, Yang SQ, Liu KC, Zhang Y, Wang BG, and Li X
- Subjects
- Animals, Fungi, Molecular Docking Simulation, Molecular Structure, Phosphatidylinositol 3-Kinases, Penicillium chemistry, Zebrafish, Terpenes chemistry, Terpenes pharmacology
- Abstract
Six new highly oxygenated and polycyclic andrastin-type meroterpenoids, namely, bialorastins A-F (1-6), were discovered from the culture of Penicillium bialowiezense CS-283, a fungus isolated from the deep-sea cold seep squat lobster Shinkaia crosnieri. The planar structures and absolute configurations of these compounds were determined by detailed analysis of spectroscopic data, single crystal X-ray diffraction, and TDDFT-ECD calculations. Structurally, bialorastin A (1) represents a rare 17-nor-andrastin that possesses an unusual 2-oxaspiro[4.5]decane-1,4-dione moiety with a unique 6/6/6/6/5 polycyclic system, while bialorastin B (2) is also a 17-nor-andrastin featuring a gem-propane-1,2-dione moiety. Additionally, bialorastins C-E (3-5) possess a 6/6/6/6/5/5 fused hexacyclic skeleton, characterized by distinctive 3,23-acetal/lactone-bridged functionalities. All isolated compounds were evaluated for their proangiogenic activities in transgenic zebrafish. Compound 3 exhibited significant proangiogenic activity, which notably increased the number and length of intersegmental blood vessels in model zebrafish in a dose-dependent manner at concentrations of 20 and 40 μM. On a molecular scale, the tested compounds were modeled through molecular docking to have insight into the interactions with the possible target VEGFR2. Mechanistically, RT-qPCR results revealed that compound 3 could promote angiogenesis via activating VEGFR2 and subsequently activating the downstream PI3K/AKT and MAPK signaling pathways. These findings indicate that 3 could be a potential lead compound for developing angiogenesis agents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
147. Antimicrobial polyketides from Magellan Seamount-derived fungus Talaromyces scorteus AS-242.
- Author
-
Wang Y, Ying Z, Li XM, Yang SQ, Li HL, Wang BG, and Meng LH
- Subjects
- Magnetic Resonance Spectroscopy methods, Molecular Structure, Polyketides chemistry, Anti-Infective Agents chemistry, Talaromyces chemistry
- Abstract
Two new nonadride derivatives, namely, talarodrides G and H (1 and 2), and one new depsidone derivative, botryorhodine K (3), together with a known nonadride analogue (4), were characterized from the Magellan Seamount-derived fungus Talaromyces scorteus AS-242. Their structures were established by detailed interpretation of NMR spectroscopic and mass spectrometry data analysis. X-ray crystallographic analysis of compounds 1 and 3 confirmed their structures and absolute configurations, representing the first characterized crystal structure of a nonadride-type polyketide. The isolated compounds exhibited potent antimicrobial activities against the pathogenic bacterium MRSA and V. parahaemolyticus and pathogenic fungi C. gloeosporioides, F. oxysporum, and F. proliferatum, with MIC values ranging from 1 to 64 μg ml
-1 ., (© 2023. The Author(s), under exclusive licence to the Japan Antibiotics Research Association.)- Published
- 2023
- Full Text
- View/download PDF
148. Tertiary lymphoid structures in gynecological cancers: prognostic role, methods for evaluating, antitumor immunity, and induction for therapy.
- Author
-
Zhang K, Xie X, Zheng SL, Deng YR, Liao D, Yan HC, Kang X, Jiang HP, and Guo SQ
- Abstract
Tertiary lymphoid structures (TLSs), referred to as tertiary lymphoid organs and lymphoid tissue neogenesis, are aggregates of immune cells that occur in nonlymphoid tissues. In recent years, it has been found that TLSs within the tumor microenvironment have been associated with local adaptive immune immunity against cancer and favorable prognosis in several human solid tumors, including gynecological cancers. The issue of the prognosis of gynecological cancers, including endometrial, cervical, and ovarian cancer, is an enormous challenge that many clinical doctors and researchers are now facing. Concerning the predictive prognostic role of TLSs, effective evaluation, and quantification of TLSs in human tissues may be used to assist gynecologists in assessing the clinical outcome of gynecological cancer patients. This review summarizes the current knowledge of TLSs in gynecological cancers, mainly focusing on the potential mechanism of TLS neogenesis, methods for evaluating TLSs, their prognostic value, and their role in antitumor immune immunity. This review also discusses the new therapeutic methods currently being explored in gynecological cancers to induce the formation of TLSs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Zhang, Xie, Zheng, Deng, Liao, Yan, Kang, Jiang and Guo.)
- Published
- 2023
- Full Text
- View/download PDF
149. A preoperative nomogram predicting risk of lymph node metastasis for early-stage cervical cancer.
- Author
-
Deng YR, Chen XJ, Xu CQ, Wu QZ, Zhang W, Guo SQ, and Li LX
- Subjects
- Female, Humans, Lymph Node Excision, Lymph Nodes pathology, Lymphatic Metastasis pathology, Neoplasm Staging, Multicenter Studies as Topic, Nomograms, Uterine Cervical Neoplasms surgery, Uterine Cervical Neoplasms pathology
- Abstract
Objective: This study aimed to develop a preoperative nomogram based on clinical and pathological characteristics to provide a more individualized and accurate estimation of lymph node metastasis (LNM) in patients with early-stage cervical cancer., Methods: A total of 7,349 early-stage cervical cancer patients with pathologically confirmed between 1988 and 2015 were obtained from the Surveillance, Epidemiology, and End Results (SEER) database. All the patients were divided into training (n = 5,500) and validation (n = 1,849) cohorts randomly. A cohort of 455 patients from multicenter was used for the external validation. We established a multivariate logistic regression model based on preoperative clinicopathological data, from which a nomogram was developed and validated. A predicted probability of LNM < 5% was defined as low risk., Results: From multivariate logistic regression analysis, age at diagnosis, histologic subtype, tumor grade, tumor size and FIGO stage were identified as preoperative independent risk factors of LNM. The nomogram incorporating these factors demonstrated good discrimination and calibration (concordance index = 0.723; 95% confidence interval (CI), 0.707-0.738). In the validation cohort, the discrimination accuracy was 0.745 (95% CI, 0.720-0.770) and 0.747 (95% CI, 0.690-0.804), respectively. The nomogram was well calibrated with a high concordance probability. We also established an R-enabled Internet browser for LNM risk assessment, which tool may be convenient for physicians., Conclusions: We developed an effective preoperative nomogram based on clinical and pathological characteristics to predict LNM for early-stage cervical cancer. This model could improve clinical trial design and help physicians to decide whether to perform lymphadenectomy or not., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
150. Tertiary Lymphoid Structures Are Associated with a Favorable Prognosis in High-Grade Serous Ovarian Cancer Patients.
- Author
-
Zhang K, Xie X, Zou LH, and Guo SQ
- Subjects
- Humans, Female, Prognosis, Gene Expression Profiling, Tumor Microenvironment, Ovarian Neoplasms pathology, Tertiary Lymphoid Structures pathology
- Abstract
There was accumulating evidence indicating that tertiary lymphoid structures (TLSs) were strongly associated with improved survival and clinical outcome in several solid tumors. In this study, we intended to assess the presence of TLSs and their potential clinical significance in high-grade serous ovarian cancer (HGSOC). TCGA (The Cancer Genome Atlas) cohort included RNA-seq data of 376 HGSOC patients, of which 74 patients included available hematoxylin-eosin (H&E) sections; GEO (Gene Expression Omnibus) cohort, GSE140082, included microarray data of 212 HGSOC patients. TLSs were counted by pathological sections, and the relative abundance of TLSs was assessed by the unsupervised consensus clustering of 12-chemokine transcriptome signatures. The potential associations between TLSs and clinical prognosis, tumor microenvironment (TME), and immunotherapy response of HGSOC were further performed based on transcriptome data. In the H&E sections of HGSOC, TLSs were predominantly located in the stroma and invasive margin of the tumor. Pathological counting results suggested that the expression of 12 chemokines was significantly higher in samples abundant with TLSs than that in the lack of TLSs. Consensus clustering of both TCGA and GEO cohorts divided HGSOC patients into two clusters with different TLSs abundance: low- and high-TLSs. Based on transcriptome analysis, the high-TLS cluster was characterized by better clinical prognosis, a higher degree of immune infiltration, more biological pathways, higher tumor mutational burden score, and higher expression of immune checkpoints. In conclusion, TLSs strongly correlated with the immune-responsive microenvironment and remained a favorable prognostic factor independent of other clinical characteristics in HGSOC. The presence of TLSs was also associated with a potentially favorable response to immune checkpoint blockade (ICB) therapy in HGSOC., (© 2023. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.