Background and objectives Various environmental and management stresses are severely threatening Iran's wetland ecosystems. As one of the most significant and strategic wetlands in the central plateau of Iran, Gavkhoni Wetland is facing various problems. Considering its particular ecological position and the diverse plant habitats around this wetland, it is essential to investigate the vegetation of this area. Knowing the different conditions of the plants, including dominant genera, geographical distribution, biological form, and conservation status, is necessary for any conservation, management, or ecological studies in these areas to be possible. Methodology The study area encompasses the upper reaches of the Zayandehrud River in Isfahan Province, covering 50,000 hectares with elevations ranging from 1,470 to 2,533 meters above sea level. This region has an arid climate characterized by saline soils, including solonchaks, marshy areas, and sandy soils. The average annual temperature at Gavkhuni Wetland is 17.6 degrees Celsius, with total annual precipitation measuring 91 millimeters. According to the Ambrothermic curve, the area experiences a prolonged dry period from April to November. Field surveys were conducted using 1:25,000 scale topographic maps from early October to November 2021. During these surveys, plant samples were collected, pressed, dried, and identified at the herbarium of the Natural Resources Department of the Isfahan University of Technology, with consultation from experts and reliable scientific sources. This research resulted in a comprehensive list of plant species detailing their scientific names, families, genera, life forms, geographical distribution, and conservation statuses. The study area of this research is located at the end of the Zayandehroud basin in Isfahan province, with an area of 500 thousand hectares and an altitude range of 1470 to 2533 meters above sea level. The region's climate is desert and dry, and saline lands with Solonchak and marshy and sandy soils are observed. The average annual temperature of Gavkhoni Lagoon is 17.6 degrees Celsius, and the average annual rainfall is 91 mm. The average annual temperature of Gavkhoni Lagoon is 17.6 degrees Celsius, and the average annual rainfall is 91 mm. According to the embrothermic curve, this region has a very long dry period from April to November. The field survey method was used to identify the area's vegetation, and maps with a 1:25000 scale were used. Field surveys and sampling were done from early October to November 1400, according to the vegetation type. After being transferred to the herbarium of the Faculty of Natural Resources of the Isfahan University of Technology, the collected plant samples were pressed, dried, identified with informed people's advice, and referred to reliable scientific sources. In this research, in addition to preparing a complete list of the plants of the Gavkhoni wetland area, the exact and new scientific names of the dominant species and genera of the area were also separated and examined. Various factors such as biological form, geographical distribution, medicinal, industrial or commercial species, and conservation status of species were investigated using reliable sources. Results This research showed that despite various environmental and management stresses, 100 plant species belonging to 16 families and 65 genera were identified in the region, of which 27 species have medicinal and industrial properties. The results indicate that Amaranthaceae, with 31 species, and Asteraceae, with 14 species, comprise 45% of the region's vegetation. Poaceae and Fabaceae families are essential in the region, with 10 and 9 species, respectively. The results of biological form showed that trophytes are the most dominant biological form, with 40%, and hemi-cryptophyte species are the most abundant at 23%. The study of the geographical distribution of plants showed that 71% of the plants in the region belong to the Iranian-Turanian vegetation zone. Different edaphic, topographical, and, to some extent, climatic conditions in this region have caused a suitable species diversity despite the harsh living conditions. Applying incorrect management measures regarding wetland water rights, climate changes, and lowering the level of underground water tables have caused environmental tensions and the drying up of some plant species in the region. According to the International Union for Conservation of Nature (IUCN) criteria, one species is endangered, and three species are vulnerable. With the continuation of unfavorable conditions imposed on the region's ecosystems (continuation of the water rights of the wetland), many existing species will suffer serious problems. Conclusion The flora at the border of Gavkhoni Lagoon is highly dependent on climate and management conditions. Despite the severe biological limitations, relatively favorable diversity was observed. Improper management in the Zayandeh Rood watershed, especially in the Gavkhoni wetland area, has caused a decrease in underground water and increased drought. Many plants in the region face drying, pest infestation, bush cutting, and overgrazing, which will be a severe threat to the plants in the region if these conditions continue and the impact of climate change. [ABSTRACT FROM AUTHOR]