615 results on '"Zini, Eric'
Search Results
102. Pheochromocytoma in dogs undergoing adrenalectomy
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Zini, Eric, Nolli, Silvia, Ferri, Filippo, Massari, Federico, Gerardi, Gabriele, Nicoli, Stefano, Romanelli, Giorgio, Montinaro, Vincenzo, Trez, Davide, Cavicchioli, Laura, Ferro, Silvia, University of Zurich, and Zini, Eric
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adrenal glands ,dogs ,scoring method ,10253 Department of Small Animals ,630 Agriculture ,3400 General Veterinary ,immunohistochemistry ,neoplasms ,570 Life sciences ,biology ,sustentacular cells ,pheochromocytoma - Published
- 2018
103. Canine leishmaniosis and kidney disease: QA for an overall management in clinical practice
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X, Roura, O, Cortadellas, M J, Day, S L, Benali, A, Zatelli, and Eric, Zini
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Dogs ,Animals ,Leishmaniasis, Visceral ,Kidney Diseases ,Dog Diseases ,Leishmania infantum ,Small Animals ,Leishmaniasis - Published
- 2020
104. Gastrointestinal Ultrasonographic Findings In Cats With Feline Panleukopenia Virus: A Case Series
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Rosaria Isaya, Stefano Ciccarelli, Daniela Enache, Swan Specchi, Marco Pesaresi, Filippo Ferri, Federico Porporato, Edoardo Auriemma, Barbara Contiero, Luigi M Coppola, and Eric Zini
- Abstract
BackgroundFeline panleukopenia virus (FPV) is very resistant and highly contagious and infects domestic cats and other felids. FPV is particularly widespread among sheltered cats, and is associated with high morbidity and mortality, causing severe gastroenteritis characterized by anorexia, lethargy, fever, dehydration, hemorrhagic diarrhea, and vomiting. There is currently no data on the ultrasonographic features of cats affected with FPV. This case series describes abdominal ultrasonographic findings in shelter cats with naturally-occurring FPV, and assesses whether are associated with clinical and laboratory findings. Cats affected by FPV were enrolled in the study if an abdominal ultrasound was performed within 12 hours of diagnosis. Clinical, laboratory and survival data were collected from medical records. Ultrasonographic examinations were reviewed for gastrointestinal abnormalities and their associations with the above data were explored.ResultsTwenty-one cats were included. Nine cats (42.9%) died and 12 (57.1%) recovered. Based on ultrasonography, the duodenum and jejunum showed thinning of the mucosal layer in 70.6% and 66.6% of cats, thickening of the muscular layer in 52.9% and 57.1% of cats, and hyperechogenicity of the mucosa in 41.2% and 33.3%. Jejunal hyperechoic mucosal band paralleling the submucosa and irregular luminal surface were both observed in 33.3% of the cats. Survival was positively associated with increased jejunal mucosal echogenicity ( P =0.003) and hyperechoic mucosal band ( P =0.003). Peritoneal free fluid was positively associated with vomiting ( P =0.002).ConclusionThis study provides ultrasonographic features of naturally-occurring FPV in cats, which, as expected, are compatible with gastroenteropathy. The most frequent findings were diffuse small intestine mucosal layer thinning, muscular layer thickening and mucosal hyperechogenicity, and jejunal hyperechoic mucosal band and irregular luminal surface. At diagnosis, ultrasonographic features may be useful to assess survival.
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- 2020
105. Computed tomographic and ultrasonographic findings of abdominal arterial pseudoaneurysms caused by systemic mycosis in dogs
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S. Morabito, Silvia Ferro, Swan Specchi, Eric Zini, Peter Kuhnert, and Edoardo Auriemma
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medicine.medical_specialty ,Systemic mycosis ,040301 veterinary sciences ,Computed tomographic ,0403 veterinary science ,Pseudoaneurysm ,Dogs ,600 Technology ,Abdomen ,medicine ,Animals ,Clinical significance ,Dog Diseases ,cardiovascular diseases ,Small Animals ,Retrospective Studies ,business.industry ,Ultrasound ,0402 animal and dairy science ,Retrospective cohort study ,04 agricultural and veterinary sciences ,Arteries ,medicine.disease ,Tunica intima ,040201 dairy & animal science ,medicine.anatomical_structure ,cardiovascular system ,570 Life sciences ,biology ,590 Animals (Zoology) ,Radiology ,business ,Tomography, X-Ray Computed ,Aneurysm, False ,Artery - Abstract
OBJECTIVES To describe multidetector CT and ultrasonographic characteristics of abdominal arterial pseudoaneurysms (segmental dilatations of an artery with a ruptured tunica intima) arising secondary to systemic mycosis in dogs. MATERIALS AND METHODS Retrospective study on dogs with confirmed histological diagnosis of a fungal pseudoaneurysm and the availability of multidetector CT or ultrasound images. RESULTS At the time of admission, the three dogs included in this study demonstrated segmental arterial dilation, irregular arterial wall thickening, and increased echogenicity or attenuation within the local perivascular fat on ultrasound and multidetector CT images. Follow-up examinations revealed progressive increase in arterial wall thickening and saccular dilation with formation of a pseudoaneurysm in affected vessels of two dogs. CLINICAL SIGNIFICANCE Multidetector CT and ultrasonography can be useful imaging modalities in the diagnosis and monitoring of abdominal arterial pseudoaneurysms caused by systemic mycosis.
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- 2020
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106. Renal AA-amyloidosis in shelter cats: a retrospective study based on clinico-pathological data, light microscopy and ultrastructural features
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Ferri, F., Palizzotto, C., Ferro, S., Benali, S., Aresu, L., Porporato, F., Rossi, F, Fiore, V., Callegari, C., Guglielmetti, C., Mazza, M., and Zini, Eric
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- 2020
107. Effect of Dietary Supplements in Reducing Probability of Death for Uremic Crises in Dogs Affected by Chronic Kidney Disease (Masked RCCT)
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Andrea Zatelli, Marco Pierantozzi, Paola D'Ippolito, Mauro Bigliati, and Eric Zini
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Technology ,Medicine ,Science - Abstract
Chitosan and alkalinizing agents can decrease morbidity and mortality in humans with chronic kidney disease (CKD). Whether this holds true in dog is not known. Objective of the study was to determine whether a commercial dietary supplement containing chitosan, phosphate binders, and alkalinizing agents (Renal), compared to placebo, reduces mortality rate due to uremic crises in dogs with spontaneous CKD, fed a renal diet (RD). A masked RCCT was performed including 31 azotemic dogs with spontaneous CKD. Dogs enrolled in the study were randomly allocated to receive RD plus placebo (group A; 15 dogs) or RD plus Renal (group B; 16 dogs). During a first 4-week period, all dogs were fed an RD and then randomized and clinically evaluated up to 44 weeks. The effects of dietary supplements on mortality rate due to uremic crises were assessed. At 44 weeks, compared to group A, dogs in group B had approximately 50% lower mortality rate due to uremic crises (P=0.015). Dietary supplementation with chitosan, phosphate binders, and alkalinizing agents, along with an RD, is beneficial in reducing mortality rate in dogs with spontaneous CKD.
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- 2012
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108. Treatment with class A CpG oligodeoxynucleotides in cats with naturally occurring feline parvovirus infection: a prospective study
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Ferri, Filippo, Porporato, Federico, Rossi, Francesco, Enache, Daniela, Callegari, Carolina, Gerardi, Gabriele, Coppola, Luigi M, Contiero, Barbara; https://orcid.org/0000-0002-6586-5035, Crinò, Chiara, Kohan, Neda Ranjbar, Meli, Marina L; https://orcid.org/0000-0002-3609-2416, Lutz, Hans, Hofmann-Lehmann, Regina; https://orcid.org/0000-0001-9750-4296, Zini, Eric; https://orcid.org/0000-0002-7580-1297, Ferri, Filippo, Porporato, Federico, Rossi, Francesco, Enache, Daniela, Callegari, Carolina, Gerardi, Gabriele, Coppola, Luigi M, Contiero, Barbara; https://orcid.org/0000-0002-6586-5035, Crinò, Chiara, Kohan, Neda Ranjbar, Meli, Marina L; https://orcid.org/0000-0002-3609-2416, Lutz, Hans, Hofmann-Lehmann, Regina; https://orcid.org/0000-0001-9750-4296, and Zini, Eric; https://orcid.org/0000-0002-7580-1297
- Abstract
Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiviral response in vivo and inhibited FPV replication in vitro. The aim was to prospectively investigate the effects of CpG-A on survival, clinical score, hematological findings, antiviral response (cytokines), viremia, and fecal shedding (real-time qPCR) in cats naturally infected with FPV. Forty-two FPV-infected cats were randomized to receive 100 µg/kg of CpG-A (n = 22) or placebo (n = 20) subcutaneously, on admission and after 48 h. Blood and fecal samples were collected on admission, after 1, 3, and 7 days. All 22 cats showed short duration pain during CpG-A injections. The survival rate, clinical score, leukocyte and erythrocyte counts, viremia, and fecal shedding at any time-point did not differ between cats treated with CpG-A (50%) and placebo (40%). Antiviral myxovirus resistance (Mx) gene transcription increased in both groups from day 1 to 3 (p = 0.005). Antibodies against FPV on admission were associated with survival in cats (p = 0.002). In conclusion, CpG-A treatment did not improve the outcome in cats with FPV infection. FPV infection produced an antiviral response.
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- 2020
109. Oxidative status of erythrocytes, hyperglycemia, and hyperlipidemia in diabetic cats
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Zini, Eric; https://orcid.org/0000-0002-7580-1297, Gabai, Gianfranco, Salesov, Elena, Gerardi, Gabriele, Da Dalt, Laura, Lutz, Thomas A; https://orcid.org/0000-0002-5056-8548, Reusch, Claudia E; https://orcid.org/0000-0002-0427-627X, Zini, Eric; https://orcid.org/0000-0002-7580-1297, Gabai, Gianfranco, Salesov, Elena, Gerardi, Gabriele, Da Dalt, Laura, Lutz, Thomas A; https://orcid.org/0000-0002-5056-8548, and Reusch, Claudia E; https://orcid.org/0000-0002-0427-627X
- Abstract
Background: Erythrocytes of diabetic cats have decreased superoxide dismutaseactivity, possibly indicative of oxidative stress. Hypothesis: Erythrocytes of diabetic cats undergo oxidative stress, which is causedby hyperglycemia and hyperlipidemia, and improves with treatment. Animals: Twenty-seven client-owned cats with diabetes mellitus, 11 matched healthycats, and 21 purpose-bred healthy cats. Methods: Prospective study. Advanced oxidized protein products, carbonyls (proteinoxidation by-products), and thiols (antioxidants) were quantified in erythrocyte mem-brane, thiobarbituric acid reactive substances (TBAR, lipid peroxidation by-products),and thiols in erythrocyte cytoplasm of all cats. Comparison were performed betweendiabetic and matched healthy cats, between diabetic cats achieving remission or not,and among purpose-bred cats after 10 days of hyperglycemia (n = 5) or hyperlipid-emia (n = 6) versus controls treated with saline (n = 5) or untreated (n = 5). Results: Compared with controls, erythrocytes of diabetic cats initially had highermedian membrane carbonyls (4.6 nmol/mg total protein [range: 0.1-37.7] versus 0.7[0.1-4.7],P< .001) and lower cytoplasmic TBAR (1.9 nmol/mg [0.5-2.4] versus 2.4[1.4-3.5]P< .001), and thiols (419 nmol/mg [165-621] versus 633 [353-824],P< 0.001). After 12-16 weeks of treatment in diabetic cats, carbonyls decreased by13% (P< .001), but remained higher (P< .001) and TBAR and thiols lower (P= .02,P< .001) than those in controls. No differences were observed between diabetic catsachieving remission or not, and among purpose-bred cats. Conclusions and Clinical Importance:Diabetes mellitus is associated with increasedprotein oxidation and reduced antioxidant defenses, which persist during treatment and remission, although mild improvement in protein oxidation occurs. Short-termhyperglycemia or hyperlipidemia does not cause oxidative stress. The reason fordecreased TBAR remains unknown.
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- 2020
110. Echocardiographic evaluation of the right ventricular dimension and systolic function in dogs with pulmonary hypertension
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Federica Marchesotti, Rosalba Tognetti, Eric Zini, Giulia Costa, María Josefa Fernández del Palacio, Luigi Venco, Tommaso Vezzosi, Oriol Domenech, University of Zurich, and Vezzosi, T
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Male ,10253 Department of Small Animals ,3400 General Veterinary ,Standard Article ,Systolic function ,030204 cardiovascular system & hematology ,0403 veterinary science ,0302 clinical medicine ,right ventricular size ,Dog Diseases ,Prospective Studies ,Prospective cohort study ,tricuspid regurgitation ,right ventricular enlargement ,Observer Variation ,Body surface area ,630 Agriculture ,Pulmonary ,04 agricultural and veterinary sciences ,Standard Articles ,congestive heart failure ,Echocardiography ,Hypertension ,Cardiology ,Veterinary (all) ,Female ,medicine.medical_specialty ,Animals ,Dogs ,Heart Ventricles ,Hypertension, Pulmonary ,Reproducibility of Results ,040301 veterinary sciences ,Regurgitation (circulation) ,Body weight ,03 medical and health sciences ,Disease severity ,Internal medicine ,medicine ,General Veterinary ,business.industry ,medicine.disease ,Pulmonary hypertension ,Heart failure ,570 Life sciences ,biology ,SMALL ANIMAL ,business - Abstract
BACKGROUND Right ventricular (RV) enlargement and dysfunction are associated with prognosis in humans with pulmonary hypertension (PH). HYPOTHESIS/OBJECTIVES To assess RV size and systolic function in dogs with PH and to determine if they are associated with disease severity and right-sided congestive heart failure (R-CHF). ANIMALS 89 dogs with PH and 74 healthy dogs. METHODS Prospective observational study. PH was classified according to the tricuspid regurgitation pressure gradient. RV end-diastolic area (RVEDA) index was calculated as RVEDA divided by body surface area. RV systolic function was assessed with the tricuspid annular plane systolic excursion (TAPSE) and the RV fractional area change (FAC) normalized for body weight (TAPSEn and FACn, respectively). RESULTS RVEDA index was higher in dogs with moderate PH (10.8 cm2 /m2 ; range, 6.2-14.4 cm2 /m2 ) and severe PH (12.4 cm2 /m2 ; range, 7.7-21.4 cm2 /m2 ) than in those with mild PH (8.4 cm2 /m2 ; range, 4.8-11.6 cm2 /m2 ) and control dogs (8.5 cm2 /m2 ; range, 2.8-11.6 cm2 /m2 ; P < .001). RVEDA index was significantly higher in dogs with R-CHF (13.7 cm2 /m2 ; range, 11.0-21.4 cm2 /m2 ) than in dogs without R-CHF (9.4 cm2 /m2 ; range, 4.8-17.1 cm2 /m2 ; P < .001). The severity of tricuspid regurgitation (TR) was the only independent predictor of the RVEDA index (P < .001). TAPSEn and FACn were not significantly different among varying degrees of PH severity and between dogs with and without R-CHF. CONCLUSIONS AND CLINICAL IMPORTANCE The RVEDA index can be used to evaluate RV size in dogs. It can provide additional information in dogs with PH and predict R-CHF. Severity of TR is the main determinant of RV enlargement in dogs with PH.
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- 2018
111. Gastrointestinal Ultrasonographic Findings In Cats With Feline Panleukopenia: A Case Series
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Isaya, Rosaria, primary, Ciccarelli, Stefano, additional, Enache, Daniela, additional, Specchi, Swan, additional, Pesaresi, Marco, additional, Ferri, Filippo, additional, Porporato, Federico, additional, Auriemma, Edoardo, additional, Contiero, Barbara, additional, Coppola, Luigi M, additional, and Zini, Eric, additional
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- 2020
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112. Glycemic variability in newly diagnosed diabetic cats treated with the glucagon‐like peptide‐1 analogue exenatide extended release
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Krämer, Anna L., primary, Riederer, Angelina, additional, Fracassi, Federico, additional, Boretti, Felicitas S., additional, Sieber‐Ruckstuhl, Nadja S., additional, Lutz, Thomas A., additional, Contiero, Barbara, additional, Zini, Eric, additional, and Reusch, Claudia E., additional
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- 2020
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113. Symmetric dimethylarginine in dogs with myxomatous mitral valve disease at various stages of disease severity
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Valente, Carlotta, primary, Guglielmini, Carlo, additional, Domenech, Oriol, additional, Contiero, Barbara, additional, Zini, Eric, additional, and Poser, Helen, additional
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- 2020
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114. Gastrointestinal Ultrasonographic Findings in Cats With Feline Panleukopenia Virus: a Case Series
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Isaya, Rosaria, primary, Ciccarelli, Stefano, additional, Enache, Daniela, additional, Specchi, Swan, additional, Pesaresi, Marco, additional, Ferri, Filippo, additional, Porporato, Federico, additional, Auriemma, Edoardo, additional, Contiero, Barbara, additional, Coppola, Luigi M, additional, and Zini, Eric, additional
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- 2020
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115. Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
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Ferri, Filippo, primary, Porporato, Federico, additional, Rossi, Francesco, additional, Enache, Daniela, additional, Callegari, Carolina, additional, Gerardi, Gabriele, additional, Coppola, Luigi M., additional, Contiero, Barbara, additional, Crinò, Chiara, additional, Kohan, Neda Ranjbar, additional, Meli, Marina L., additional, Lutz, Hans, additional, Hofmann-Lehmann, Regina, additional, and Zini, Eric, additional
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- 2020
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116. Oxidative status of erythrocytes, hyperglycemia, and hyperlipidemia in diabetic cats
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Zini, Eric, primary, Gabai, Gianfranco, additional, Salesov, Elena, additional, Gerardi, Gabriele, additional, Da Dalt, Laura, additional, Lutz, Thomas A., additional, and Reusch, Claudia E., additional
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- 2020
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117. A Clinical, Radiographic and Histological Study of Unerupted Teeth in Dogs and Cats: 73 Cases (2001–2018)
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Bellei, Emma, primary, Ferro, Silvia, additional, Zini, Eric, additional, and Gracis, Margherita, additional
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- 2019
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118. Feline diabetes mellitus: background
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Zini, Eric, University of Zurich, and Zini, Eric
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10253 Department of Small Animals ,630 Agriculture ,570 Life sciences ,biology - Published
- 2018
119. Insulin preparations: which one to choose?
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Zini, Eric, University of Zurich, and Zini, Eric
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10253 Department of Small Animals ,630 Agriculture ,570 Life sciences ,biology - Published
- 2018
120. European Veterinary Renal Pathology Service: A Survey Over a 7-Year Period (2008-2015)
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Davide Trez, Silvia Benali, J.J. van der Lugt, Eric Zini, R. Dalla Riva, C. Brovida, Valeria Martini, Luca Aresu, A.M. van Dongen, Rachel E. Cianciolo, University of Zurich, and Aresu, L
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Male ,0301 basic medicine ,Veterinary medicine ,10253 Department of Small Animals ,3400 General Veterinary ,Biopsy ,Standard Article ,Kidney ,0403 veterinary science ,Glomerulonephritis ,Surveys and Questionnaires ,Diagnosis ,Dog ,Nephrology/Urology ,Minimal change disease ,Dog Diseases ,Registries ,Hypoalbuminemia ,Microscopy ,630 Agriculture ,medicine.diagnostic_test ,Not Otherwise Specified ,04 agricultural and veterinary sciences ,Standard Articles ,Europe ,medicine.anatomical_structure ,Renal pathology ,Veterinary (all) ,Female ,Kidney Diseases ,Renal biopsy ,040301 veterinary sciences ,Nephropathy ,03 medical and health sciences ,Dogs ,Electron microscopy ,medicine ,Animals ,General Veterinary ,business.industry ,medicine.disease ,Microscopy, Electron ,030104 developmental biology ,570 Life sciences ,biology ,SMALL ANIMAL ,business - Abstract
Background The European Veterinary Renal Pathology Service (EVRPS) is the first Web-based registry for canine renal biopsy specimens in Europe. Hypothesis/Objectives The aim was to verify whether differences exist between the clinical and laboratory presentation of dogs with nephropathy according to renal pathological findings, as defined by light and electron microscopy of renal biopsy specimens submitted to EVRPS. Animals Renal biopsy specimens of dogs were collected from the archive of the service (n = 254). Cases were included if both light and electron microscopy were available (n = 162). Methods Renal biopsy specimens were classified based on the morphological diagnoses. Thereafter, they were grouped into 3 disease categories, including immune-complex-mediated glomerulonephritis (ICGN), non-immune-complex-mediated GN (non-ICGN), and renal lesions not otherwise specified (RL-NOS). Differences among morphological diagnoses and among disease categories were investigated for clinical and laboratory variables. Results Serum albumin concentration was lower in dogs with ICGN than in those with non-ICGN (P = 0.006) or RL-NOS (P = 0.000), and the urine protein-to-creatinine ratio (UPC) was significantly higher in ICGN than in the other 2 disease categories. Regarding morphological diagnoses, albumin was significantly lower in amyloidosis (AMY) and membranous (MGN), membranoproliferative (MPGN) or mixed glomerulonephritis (MixGN) than in minimal change disease, primary (FSGS I) or secondary (FSGS II) focal and segmental glomerulosclerosis and juvenile nephropathies (JN). The UPC was higher in MPGN than in FSGS I and FSGS II. Conclusions and clinical importance Dogs with ICGN, in particular MPGN, had higher protein loss than those with non-ICGN or RL-NOS, leading to more severe hypoalbuminemia. Clinical and laboratory differentiation among dogs with the different morphological diagnoses and among dogs with different disease categories was difficult due to overlapping results.
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- 2017
121. Research Communications of the 26th ECVIM-CA Congress
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Federica Marchesotti, Oriol Domenech, Tommaso Vezzosi, and Eric Zini
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0301 basic medicine ,Aorta ,CATS ,General Veterinary ,business.industry ,030231 tropical medicine ,Two dimensional echocardiography ,Left atrium ,Anatomy ,Abstracts ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,medicine.artery ,medicine ,business - Published
- 2017
122. Diabetic cats have decreased gut microbial diversity and a lack of butyrate producing bacteria
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Ida N. Kieler, Torben Hansen, Eric Zini, Charlotte R. Bjornvad, Lara Puetz, Oluf Pedersen, Thomas A. Lutz, M. Thomas P. Gilbert, Claudia E Reusch, Melania Osto, Leoni Hugentobler, University of Zurich, and Kieler, Ida Nordang
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Male ,0301 basic medicine ,10253 Department of Small Animals ,Denmark ,Physiology ,lcsh:Medicine ,Gut flora ,Overweight ,Prevotellaceae ,Cat Diseases ,Feces ,0302 clinical medicine ,RNA, Ribosomal, 16S ,Prospective Studies ,lcsh:Science ,CATS ,Multidisciplinary ,biology ,10081 Institute of Veterinary Physiology ,Butyrates ,Diet, High-Protein ,Fructosamine ,Female ,medicine.symptom ,Switzerland ,DNA, Bacterial ,Butyrate ,Article ,03 medical and health sciences ,Diabetes mellitus ,Diabetes Mellitus ,medicine ,Animals ,DNA Barcoding, Taxonomic ,Obesity ,1000 Multidisciplinary ,Bacteria ,lcsh:R ,nutritional and metabolic diseases ,biology.organism_classification ,medicine.disease ,Gastrointestinal Microbiome ,Cross-Sectional Studies ,030104 developmental biology ,Cats ,Dysbiosis ,570 Life sciences ,lcsh:Q ,030217 neurology & neurosurgery - Abstract
Obesity and inactivity are major risk factors of feline diabetes mellitus (FDM) and human type II diabetes mellitus (T2DM). In recent years, changes in the gut microbiota have been suggested as a contributing factor to T2DM. Whether the gut microbiota (GM) composition plays a role in FDM remains unknown. The aim of the current study was firstly a cross-sectional comparison of the GM of diabetic cats, to that of lean, and of obese/overweight non-diabetic cats of a similar age. Specifically, fecal samples from 82 privately-owned cats from Denmark and Switzerland were sequenced using 16S rRNA gene amplicon metabarcoding. Secondly dietary intervention data was generated, by obtaining additional samples from a subset of cats after placing them on a high-protein diet for four weeks. The GM diversity of diabetic cats was lower than that of lean cats in the cross-sectional study, and lower compared to lean and to overweight/obese cats after diet intervention. Diabetic cats also exhibited fewer Anaerotruncus, Dialister, and unknown Ruminococcaceae than lean cats. Serum fructosamine levels correlated negatively with Prevotellaceae abundance and positively with Enterobacteriaceae abundance. In summary the intestinal microbiota of diabetic cats was characterized by decreased GM diversity and loss of butyrate producing bacterial genera.
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- 2019
123. Left atrial anteroposterior diameter in dogs: Reference interval, allometric scaling, and agreement with the left atrial-to-aortic root ratio
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Eric Zini, Federica Marchesotti, Oriol Domenech, Tommaso Vezzosi, Francesca Marchetti, Rosalba Tognetti, V. Patata, and Barbara Contiero
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Male ,medicine.medical_specialty ,Myxomatous mitral valve disease ,040301 veterinary sciences ,Aortic root ,Cardiology ,Normal Reference Range ,Reference range ,Body weight ,Canine ,Degenerative valvular disease ,0403 veterinary science ,03 medical and health sciences ,Dogs ,Left atrial ,Internal medicine ,Internal Medicine ,Left atrial enlargement ,medicine ,Animals ,Aorta ,030304 developmental biology ,Retrospective Studies ,0303 health sciences ,Mitral Valve Prolapse ,Full Paper ,General Veterinary ,business.industry ,Significant difference ,Heart ,04 agricultural and veterinary sciences ,Echocardiography ,Case-Control Studies ,Female ,medicine.disease ,canine ,cardiology ,degenerative valvular disease ,echocardiography ,myxomatous mitral valve disease ,Allometry ,business - Abstract
Left atrial enlargement (LAE) is a well-known negative prognostic factor in dogs with myxomatous mitral valve disease (MMVD). Left atrial-to-aortic root ratio (LA/Ao) is the most commonly used method to evaluate left atrial (LA) size in dogs, while the left atrial anteroposterior diameter (LAD) has been proposed as an additional measurement of LA size in different species. The aim of this study was to establish a normal reference range of LAD normalized to body weight (LADn) in dogs using allometric scales, and to evaluate the agreement between LADn and LA/Ao in the detection of LAE in dogs with MMVD. This was a retrospective, multicenter, observational study. We included 330 healthy dogs, 30 dogs with MMVD in ACVIM stage B1, 30 dogs in ACVIM stage B2, and 30 dogs in ACVIM stage C. The reference range for the LAD, depending on body weight, was between 16.91 mm and 49.68 mm. The reference range for the LADn in healthy dogs was between 10.49 and 15.72. LADn was significantly greater in dogs with MMVD compared to healthy dogs, and a significant difference in LADn was noted between different ACVIM stages (P
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- 2019
124. Home monitoring of heart rate and heart rhythm with a smartphone-based ECG in dogs
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Tommaso Vezzosi, Eric Zini, V. Patata, Carlotta Buralli, Rosalba Tognetti, Oriol Domenech, and Federica Marchesotti
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Adult ,Male ,medicine.medical_specialty ,040301 veterinary sciences ,electrocardiography ,alivecor ,arrhythmia ,iphone ,owner ,0403 veterinary science ,Rhythm ,Dogs ,Heart Rate ,Heart rate ,Medicine ,Animals ,Humans ,Dog Diseases ,Monitoring, Physiologic ,General Veterinary ,medicine.diagnostic_test ,business.industry ,0402 animal and dairy science ,Arrhythmias, Cardiac ,04 agricultural and veterinary sciences ,General Medicine ,Middle Aged ,040201 dairy & animal science ,Home Care Services ,Heart Rhythm ,First person ,Physical therapy ,Veterinary (all) ,Feasibility Studies ,Female ,Smartphone ,business ,Electrocardiography - Abstract
The feasibility of the home monitoring of heart rate (HR) and rhythm through ECG tracings recorded by owners with a smartphone ECG device was evaluated in dogs. Smartphone ECG tracings were recorded by owners at home using a single-lead ECG device and sent via email for interpretation. A questionnaire was prepared to assess the owner’s opinion regarding this home monitoring service. Recordings were evaluated by two operators, and agreement was evaluated for HR and rhythm diagnosis. Thirty-three dogs were included. Thirty-one owners (94 per cent) felt that the recording technique was easy to learn and that the smartphone ECG device was easy to use. A total of 15 owners (45 per cent) required a second person to hold the dog during recording. Of the 150 smartphone ECG tracings that were received, 134 (89 per cent) were interpretable. The median difference between the two operators to assess the mean HR on the smartphone tracings was 10 bpm (−10, +25 bpm). Perfect agreement (κ=1) between operators was observed in the heart rhythm evaluation. Most owners sent adequate ECG tracings for interpretation via email from their smartphone. Home monitoring of HR and heart rhythm may represent an additional tool in the management of dogs with arrhythmias.
- Published
- 2019
125. Immune-complex glomerulonephritis in cats: A retrospective study based on clinico-pathological data, histopathology and ultrastructural features
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Eric Zini, Francesco Rossi, Davide Trez, Valeria Martini, Luca Aresu, Rossella Zanetti, Luigi Michele Coppola, and Filippo Ferri
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Male ,medicine.medical_specialty ,Feline immunodeficiency virus ,040301 veterinary sciences ,Cat Diseases ,Kidney ,Gastroenterology ,0403 veterinary science ,Feline ,03 medical and health sciences ,chemistry.chemical_compound ,Focal segmental glomerulosclerosis ,Glomerulonephritis ,Immune-deposits ,Proteinuria ,Transmission electron microscopy ,Internal medicine ,Membranoproliferative glomerulonephritis ,medicine ,Animals ,Immune Complex Diseases ,030304 developmental biology ,Retrospective Studies ,0303 health sciences ,Creatinine ,lcsh:Veterinary medicine ,CATS ,biology ,General Veterinary ,business.industry ,04 agricultural and veterinary sciences ,General Medicine ,medicine.disease ,biology.organism_classification ,chemistry ,Cats ,lcsh:SF600-1100 ,Female ,medicine.symptom ,business ,Kidney disease ,Research Article - Abstract
Background Chronic kidney disease (CKD) has typically a non-immune mediated origin in cats and immune-complex glomerulonephritis (ICGN) is scarcely described. Aims of this study were to characterize ICGN by light and electron microscopy and identify associations with clinico-pathological findings. In addition, comparisons between cats with ICGN and non immune-complex glomerulonephritis (non-ICGN) were performed. Renal samples examined between 2010 and 2019 were considered if both light and electron microscopy were performed. Signalment, feline immunodeficiency virus (FIV) and leukemia virus (FeLV) status, serum creatinine concentration, urine protein-to-creatinine (UPC) ratio, systolic blood pressure (SBP) and International Renal Interest Society (IRIS) stage were retrieved and used for comparisons. Results Sixty-eight client-owned cats were included. Thirty-seven cats (54.4%) had ICGN and 31 (45.6%) non-ICGN. Eighteen (48.6%) with ICGN had membranous glomerulonephropathy (MGN), 14 (37.8%) membranoproliferative glomerulonephritis (MPGN), and 5 (13.5%) mesangioproliferative glomerulonephritis (MeGN). Clinico-pathological data were not associated with any type of ICGN. Among cats with non-ICGN, 11 (35.5%) had end-stage CKD, 9 (29%) focal segmental glomerulosclerosis, 6 (19.4%) global and multifocal mesangiosclerosis, 2 (6.5%) glomerular atrophy, 2 (6.5%) renal dysplasia and 1 (3.1%) amyloidosis. Eight (25.8%) cats with non-ICGN had chronic interstitial nephritis (CIN) grade 1, 13 (41.9%) grade 2 and 10 (32.3%) grade 3; creatinine and UPC ratio increased with CIN grades (p = 0.001, p
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- 2019
126. Endocrine Pancreas in Cats With Diabetes Mellitus
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Zini, Eric, Lunardi, F, Zanetti, R, Heller, R S, Coppola, L M, Ferro, S, Guscetti, Franco, Osto, Melania, Lutz, Thomas A, Reusch, Claudia E, Cavicchioli, L, University of Zurich, and Zini, Eric
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islets ,Male ,endocrine system ,10253 Department of Small Animals ,General Veterinary ,3400 General Veterinary ,amyloid ,cat ,10184 Institute of Veterinary Pathology ,Amyloidosis ,10081 Institute of Veterinary Physiology ,Cat Diseases ,Glucagon ,Islet Amyloid Polypeptide ,Islets of Langerhans ,Cats ,Diabetes Mellitus ,570 Life sciences ,biology ,Animals ,Insulin ,pathology ,Female ,diabetes mellitus ,pancreas ,Pancreas - Abstract
Pancreatic amyloidosis and loss of α and β cells have been shown to occur in cats with diabetes mellitus, although the number of studies currently available is very limited. Furthermore, it is not known whether pancreatic islet inflammation is a common feature. The aims of the present study were to characterize islet lesions and to investigate whether diabetic cats have inflammation of the pancreatic islets. Samples of pancreas were collected postmortem from 37 diabetic and 20 control cats matched for age, sex, breed, and body weight. Histologic sections were stained with hematoxylin and eosin and Congo red; double labeled for insulin/CD3, insulin/CD20, insulin/myeloperoxidase, insulin/proliferating cell nuclear antigen, and glucagon/Ki67; and single labeled for amylin and Iba1. Mean insulin-positive cross-sectional area was approximately 65% lower in diabetic than control cats ( P = .009), while that of amylin and glucagon was similar. Surprisingly, amyloid deposition was similar between groups ( P = .408). Proliferation of insulin- and glucagon-positive cells and the number of neutrophils, macrophages, and T (CD3) and B (CD20) lymphocytes in the islets did not differ. The presence of T and B lymphocytes combined tended to be more frequent in diabetic cats ( n = 8 of 37; 21.6%) than control cats ( n = 1 of 20; 5.0%). The results confirm previous observations that loss of β cells but not α cells occurs in diabetic cats. Islet amyloidosis was present in diabetic cats but was not greater than in controls. A subset of diabetic cats had lymphocytic infiltration of the islets, which might be associated with β-cell loss.
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- 2015
127. COMPARISON OF GLOMERULAR NUMBER AND SPECIMEN LENGTH OBTAINED FROM 100 DOGS VIA PERCUTANEOUS ECHO-ASSISTED RENAL BIOPSY USING TWO DIFFERENT NEEDLES
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Zatelli, Andrea, DʼIppolito, Paola, and Zini, Eric
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- 2005
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128. Comparison of primary mitral valve disease in German Shepherd dogs and in small breeds
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Borgarelli, Michele, Zini, Eric, D'Agnolo, Gino, Tarducci, Alberto, Santilli, Roberto A., Chiavegato, David, Tursi, Massimo, Prunotto, Marco, and Häggström, Jens
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- 2004
- Full Text
- View/download PDF
129. 64-multidetector CT anatomical assessment of the feline bronchial and pulmonary vascular structures
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Panopoulos, I; https://orcid.org/0000-0001-8496-2159, Auriemma, E, Specchi, S, Diana, A, Pietra, M, Papastefanou, A, Zini, Eric; https://orcid.org/0000-0002-7580-1297, Cipone, M, Panopoulos, I; https://orcid.org/0000-0001-8496-2159, Auriemma, E, Specchi, S, Diana, A, Pietra, M, Papastefanou, A, Zini, Eric; https://orcid.org/0000-0002-7580-1297, and Cipone, M
- Abstract
OBJECTIVES: The aim of the study was to provide a detailed anatomical study of the feline bronchial and vascular structures by using CT angiography (CTA). METHODS: Adult cats with no respiratory clinical signs were enrolled in a CTA protocol to provide an anatomical study of the thorax. The dimensions, number of branches and branching pattern (monopodial vs dichotomic) of both bronchial and pulmonary vascular structures were evaluated under positive inspiration apnoea. A linear generalised estimating equations analysis (Spearman's rho) was used to identify statistical correlation between tracheal diameter, age and body weight of the cats. RESULTS: Fourteen cats met the inclusion criteria. The pulmonary arteries had larger diameters than the pulmonary veins, and the pulmonary veins had larger diameters than the bronchial structures. A higher number of segmental bronchial and pulmonary vascular branches was observed in the left caudal lung lobe than in the other lobes. The monopodial branching pattern of both bronchial and pulmonary vascular structures was predominant in all cats of our study (100%) in cranial, caudal and right middle lung lobes, while a dichotomic branching pattern of the bronchial and pulmonary vascular structures of the accessory lung lobe was seen in 13 cats (93%). Thirteen cats (93%) had three pulmonary vein ostia, and one cat (7%) also presented with an additional left intermediate pulmonary vein ostium. Variation in the number of segmental pulmonary vein branches was noted in the right caudal lung lobe. There was no statistical correlation between tracheal diameter, age and weight. CONCLUSIONS AND RELEVANCE: Architecture of the feline bronchovascularr structures belongs to a mixed type of monopodial and dichotomic branching pattern. In cats, the pulmonary venous drainage system predominately presents three pulmonary vein ostia. Variations in the type of formation and the number of branches of the pulmonary venous drainage system were noted.
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- 2019
130. Glucose concentrations after insulin-induced hypoglycemia and glycemic variability in healthy and diabetic cats
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Barbara Contiero, Claudia E Reusch, Thomas A. Lutz, Laura Moretto, Eric Zini, Perrine Dupont, Elena Salesov, University of Zurich, and Zini, Eric
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Blood Glucose ,Male ,medicine.medical_specialty ,10253 Department of Small Animals ,040301 veterinary sciences ,Insulin induced hypoglycemia ,medicine.medical_treatment ,3400 General Veterinary ,cat ,diabetes mellitus ,endocrinology ,pancreas ,Animals ,Case-Control Studies ,Cat Diseases ,Cats ,Diabetes Mellitus ,Female ,Hypoglycemia ,Insulin ,Veterinary (all) ,030209 endocrinology & metabolism ,Standard Article ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,Diabetes mellitus ,medicine ,Glycemic ,CATS ,General Veterinary ,630 Agriculture ,business.industry ,Glucose meter ,04 agricultural and veterinary sciences ,medicine.disease ,10081 Institute of Veterinary Physiology ,Standard Articles ,medicine.anatomical_structure ,570 Life sciences ,biology ,SMALL ANIMAL ,Pancreas ,business - Abstract
Background Little information is available about posthypoglycemic hyperglycemia (PHH) in diabetic cats, and a causal link between hypoglycemia and subsequent hyperglycemia is not clear. Fluctuations in blood glucose concentrations might only represent high glycemic variability. Hypothesis Insulin induces PHH in healthy cats, and PHH is associated with poorly regulated diabetes and increased glycemic variability in diabetic cats. Animals Six healthy cats, 133 diabetic cats. Methods Insulin (protamine‐zinc and degludec; 0.1‐0.3 IU/kg) administered to healthy cats. Blood glucose curves were generated with portable glucose meter to determine the percentage of curves with PHH. Data from insulin‐treated diabetic cats with blood glucose curves showing hypoglycemia included data of cats with and without PHH. Post‐hypoglycemic hyperglycemia was defined as blood glucose concentrations 15 mmol/L within 12 hours. Glycemic variability was calculated as the standard deviation of the blood glucose concentrations. Results In healthy cats, all insulin doses caused hypoglycemia but PHH was not observed; glycemic variability did not differ between insulin preparations. Among diabetic cats with hypoglycemia, 33 (25%) had PHH. Compared with cats without PHH, their daily insulin dose was higher (1.09 ± 0.55 versus 0.65 ± 0.56 IU/kg; P
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- 2017
131. Electrocardiogram-gated 16-multidetector computed tomographic angiography of the coronary arteries in dogs
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Oriol Domenech, Swan Specchi, Edoardo Auriemma, Simona Morabito, Vincenzo Rondelli, Giuseppe Lacava, Eric Zini, Felice Armienti, Toufic Khouri, Carlo Guglielmini, University of Zurich, and Zini, Eric
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Coronary angiography ,Male ,medicine.medical_specialty ,10253 Department of Small Animals ,040301 veterinary sciences ,Computed Tomography Angiography ,3400 General Veterinary ,030204 cardiovascular system & hematology ,computed tomography (CT) ,Coronary Angiography ,0403 veterinary science ,03 medical and health sciences ,Electrocardiography ,0302 clinical medicine ,Left coronary artery ,Dogs ,cardiology ,dogs ,imaging ,Medicine (all) ,Internal medicine ,medicine.artery ,Multidetector Computed Tomography ,medicine ,Animals ,General Veterinary ,630 Agriculture ,business.industry ,04 agricultural and veterinary sciences ,General Medicine ,Coronary Vessels ,Computed tomographic angiography ,Coronary arteries ,Coding system ,medicine.anatomical_structure ,Right coronary artery ,Cardiology ,570 Life sciences ,biology ,Female ,business ,Artery - Abstract
The aims of this study were to assess if ECG-gated 16-multidetector CT (MDCT) provides sufficient temporal and spatial resolution to evaluate canine coronary arteries and provide a detailed description of their anatomy. A total of 24 dogs were included. Images were reviewed to assess: (1) coronary artery opacification and dominance; (2) choice of optimal R-R ECG reconstruction interval for both left coronary artery (LCA) and right coronary artery (RCA); (3) branching patterns of the left main coronary artery (LMCA); and (4) diameter and length of the LCA and RCA and classification of their branches by adapting a previously described segmental coding system. The degree of opacification of the coronary arteries was subjectively judged as excellent or good in five and 19 dogs, respectively. All hearts showed a left coronary dominance. The best R-R reconstruction interval for both LCA and RCA arteries was 75 per cent. Seven different subtypes of LMCA branching patterns were noted. The rami circumflexus and interventricularis paraconalis were divided into three angiographic segments, and the ramus septi interventricularis and the RCA in two and three segments, respectively. ECG-gated 16-MDCT coronary angiography provides adequate resolution to assist the basic anatomy of the main coronary artery branches.
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- 2017
132. Computed tomographic and angiographic assessment of spinal extradural arteriovenous fistulas in a dog
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Morabito, Simona, Edoardo, Auriemma, Paolo, Zagarella, Edy, Mercuriali, Oriol, Domenech, Gualtiero, Gandini, Teresa, Gagliardo, Eric, Zini, Swan, Specchi, University of Zurich, Specchi, S, Morabito, Simona, Auriemma, Edoardo, Zagarella, Paolo, Mercuriali, Edy, Domenech, Oriol, Gandini, Gualtiero, Gagliardo, Teresa, Zini, Eric, and Specchi, Swan
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Male ,SHUNTS ,10253 Department of Small Animals ,630 Agriculture ,3400 General Veterinary ,Angiography ,Scientific ,Thoracic Vertebrae ,Dogs ,Paraparesis ,CORD ,Arteriovenous Fistula ,MANAGEMENT ,570 Life sciences ,biology ,Animals ,Dog Diseases ,Tomography, X-Ray Computed ,VASCULAR MALFORMATIONS ,Spinal Canal ,Spinal Cord Compression - Abstract
A 6-month-old German shepherd dog was presented for progressive paraparesis. Multiple arteriovenous fistulae and hyperostosis of the thoracic vertebrae with secondary thoraco-lumbar spinal cord compression were diagnosed. Arteriovenous spinal fistula is a rare condition but should be considered as a differential diagnosis in young dogs with progressive paraparesis.
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- 2017
133. Use of an implantable pump for controlled subcutaneous insulin delivery in healthy cats
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A Riederer, I Padrutt, K Macha, Eric Zini, Thomas A. Lutz, Claudia E Reusch, Marco Pesaresi, University of Zurich, and Zini, Eric
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Male ,10253 Department of Small Animals ,medicine.medical_treatment ,3400 General Veterinary ,Insulin Glargine ,0403 veterinary science ,Diabetes mellitus ,0302 clinical medicine ,Bolus (medicine) ,Controlled delivery ,Insulin ,Infusion Pumps ,CATS ,630 Agriculture ,Subcutaneous ,Cat ,04 agricultural and veterinary sciences ,Infusion Pumps, Implantable ,10081 Institute of Veterinary Physiology ,Implantable pump ,Treatment ,Animals ,Cats ,Hypoglycemic Agents ,Injections, Subcutaneous ,Insulin Infusion Systems ,Reproducibility of Results ,Animal Science and Zoology ,Veterinary (all) ,Anesthesia ,Implantable ,medicine.drug ,medicine.medical_specialty ,040301 veterinary sciences ,030209 endocrinology & metabolism ,Injections ,03 medical and health sciences ,medicine ,Implantable Pump ,General Veterinary ,business.industry ,Insulin glargine ,medicine.disease ,Subcutaneous insulin ,Surgery ,570 Life sciences ,biology ,1103 Animal Science and Zoology ,business - Abstract
The aim of this study was to examine the safety and reliability of a research-grade implantable pump for controlled delivery of insulin glargine in cats. For this purpose, a small telemetrically controlled drug delivery pump with a refillable reservoir was implanted into the subcutaneous tissues of the dorsal neck in 10 clinically healthy cats. The reservoir was filled with insulin glargine, and the pump was programmed to deliver four boluses of 0.25 IU/kg, 2-3 weeks apart. As a control, insulin glargine (0.25 IU/kg) was injected SC. Blood glucose and plasma insulin glargine concentrations were measured before each bolus and SC injection and for 8 h afterward. Cats were monitored for signs of discomfort. Pumps were easily implanted and well tolerated by all cats. The experiment was completed in five of 10 cats. In four, the pump failed because of technical reasons; another cat developed severe hypoglycaemia attributable to insulin leakage. Overall, plasma insulin glargine increased after six of eight (75%) initial boluses and after one of 16 (6%) successive boluses. Glucose decreased after seven of eight (88%) initial boluses and after four of 16 (25%) successive boluses. Only the first bolus significantly increased plasma insulin glargine (P = 0.008) and decreased glucose (P = 0.008). Of 20 SC injections, 10 (50%) increased plasma insulin glargine (P
- Published
- 2017
134. EVALUATION OF THE COMPUTED TOMOGRAPHIC 'SENTINEL CLOT SIGN' TO IDENTIFY BLEEDING ABDOMINAL ORGANS IN DOGS WITH HEMOABDOMEN
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Eric Zini, Simona Morabito, Swan Specchi, Edoardo Auriemma, Federica Rossi, Filippo Ferri, Pascaline Pey, and Valentina Piola
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medicine.medical_specialty ,General Veterinary ,040301 veterinary sciences ,business.industry ,Hemoabdomen ,030208 emergency & critical care medicine ,04 agricultural and veterinary sciences ,Abdominal cavity ,medicine.disease ,Extravasation ,Computed tomographic ,0403 veterinary science ,03 medical and health sciences ,Contrast medium ,0302 clinical medicine ,Hematoma ,medicine.anatomical_structure ,medicine ,Ct findings ,Radiology ,business ,Sign (mathematics) - Abstract
The CT "sentinel clot sign" has been defined as the highest attenuation hematoma adjacent to a bleeding organ in humans with hemoabdomen. The aims of this retrospective descriptive multicenter study were to describe CT findings in a sample of dogs with surgically or necropsy confirmed intra-abdominal bleeding and determine prevalence of the "sentinel clot sign" adjacent to the location of bleeding. Medical records between 2012 and 2014 were searched for dogs with hemoabdomen and in which the origin of the bleeding was confirmed either with surgery or necropsy. Retrieved CT images were reviewed for the presence and localization of the "sentinel clot sign," HU measurements of the "sentinel clot sign" and hemoabdomen, and presence of extravasation of contrast media within the abdominal cavity. Nineteen dogs were included. Three dogs were excluded due to the low amount of blood that did not allow the identification of a "sentinel clot sign." A "sentinel clot sign" was detected in the proximity of the confirmed bleeding organ in 14/16 (88%) of the patients. The mean HU of the "sentinel clot sign" was 56 (range: 43-70) while that of the hemoabdomen was 34 (range: 20-45). Active hemorrhage was identified as extravasation of contrast medium within the peritoneal cavity from the bleeding organ in three dogs. In conclusion, the CT "sentinel clot sign" may be helpful for identifying the source of bleeding in dogs with hemoabdomen.
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- 2016
135. Evaluation of insulin-like growth factor-1, total thyroxine, feline pancreas-specific lipase and urinary corticoid-to-creatinine ratio in cats with diabetes mellitus in Switzerland and the Netherlands
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Hans S. Kooistra, Barbara Riond, Sandra Schaefer, Jan S. Suchodolski, Claudia E Reusch, Eric Zini, Jörg M. Steiner, Marrit Prins, University of Zurich, and Reusch, Claudia E
- Subjects
Male ,10253 Department of Small Animals ,Physiology ,Urine ,Cat Diseases ,Hyperthyroidism ,0403 veterinary science ,chemistry.chemical_compound ,0302 clinical medicine ,Adrenal Cortex Hormones ,Prevalence ,Insulin-Like Growth Factor I ,Small Animals ,Cushing Syndrome ,Netherlands ,Acromegaly ,Animals ,Cats ,Creatinine ,Diabetes Mellitus ,Female ,Lipase ,Pancreatitis ,Switzerland ,Thyroxine ,CATS ,630 Agriculture ,04 agricultural and veterinary sciences ,Fructosamine ,medicine.drug ,medicine.medical_specialty ,040301 veterinary sciences ,Urinary system ,030209 endocrinology & metabolism ,03 medical and health sciences ,Diabetes mellitus ,Internal medicine ,medicine ,Insulin glargine ,business.industry ,medicine.disease ,10187 Department of Farm Animals ,Endocrinology ,chemistry ,3404 Small Animals ,570 Life sciences ,biology ,business - Abstract
Objectives The aim of the study was to evaluate circulating insulin-like growth factor-1 (IGF-1), feline pancreas-specific lipase (fPLI) and total thyroxine (TT4) concentrations and urinary corticoid-to-creatinine ratio (UCCR) as indicators for the prevalence of acromegaly, pancreatitis, hyperthyroidism and hypercortisolism in cats with diabetes mellitus. Methods Blood and urine samples were collected from diabetic cats treated in primary care clinics in Switzerland and the Netherlands. Standardised questionnaires and physical examination forms provided clinical information from owners and veterinarians. Laboratory testing included serum biochemistry profile analysis and measurement of circulating fructosamine, IGF-1, fPLI, and TT4 concentrations and UCCR. CT of the pituitary gland was performed using a multidetector computed tomography scanner. Results Blood samples were available from 215 cats and urine samples were collected at home from 117 cats. Age ranged from 2–18 years (median 12 years) and body weight from 2.7–12.3 kg (median 5.5 kg). Sixty-five percent of the cats were castrated male and 35% were female (33% spayed); 82% were domestic shorthair cats. Eighty percent of cats received a porcine insulin zinc suspension, 19.5% insulin glargine and 0.5% a human neutral protamine hagedorn insulin. Thirty-six of 202 (17.8%) cats had IGF-1 concentrations >1000 ng/ml. Serum fPLI, and TT4 concentrations and UCCR were increased in 86/196 (43.9%), 9/201 (4.5%) and 18/117 cats (15.3%), respectively. Prevalence did not differ between countries. Conclusions Hyperthyroidism is rare, whereas increased fPLI concentration, possibly reflecting pancreatitis, is common in diabetic cats. The high UCCR may reflect activation of the hypothalamus–pituitary–adrenal axis, which also occurs in diabetic humans. The percentage of cats with increased IGF-1 was high but lower than reported in recent studies.
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- 2016
136. Effect of the Glucagon‐like Peptide‐1 Analogue Exenatide Extended Release in Cats with Newly Diagnosed Diabetes Mellitus
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Eric Zini, T M Stöckle, Thomas A. Lutz, K Macha, Federico Fracassi, A Riederer, I Padrutt, Elena Salesov, Claudia E Reusch, University of Zurich, Reusch, Claudia E, Riederer, A., Zini, E., Salesov, E., Fracassi, F., Padrutt, I., Macha, K., Stöckle, T.M., Lutz, T.A., and Reusch, C.E
- Subjects
Blood Glucose ,Male ,10253 Department of Small Animals ,3400 General Veterinary ,medicine.medical_treatment ,Insulin Glargine ,Standard Article ,Cat Diseases ,0403 veterinary science ,Endocrinology ,CATS ,630 Agriculture ,Glucagon secretion ,04 agricultural and veterinary sciences ,Incretin ,10081 Institute of Veterinary Physiology ,Glucagon-like peptide-1 ,Standard Articles ,Veterinary (all) ,Female ,medicine.drug ,medicine.medical_specialty ,Remission ,040301 veterinary sciences ,Placebo ,Metabolic control ,Feline ,Internal medicine ,Diabetes Mellitus ,Dietary Carbohydrates ,medicine ,Animals ,Hypoglycemic Agents ,General Veterinary ,Venoms ,Insulin glargine ,business.industry ,Insulin ,0402 animal and dairy science ,Animal Feed ,040201 dairy & animal science ,Hypoglycemia ,Diet ,Treatment ,Cats ,Exenatide ,570 Life sciences ,biology ,SMALL ANIMAL ,Peptides ,business - Abstract
Background Exenatide extended release (ER) is a glucagon-like peptide-1 analogue that increases insulin secretion, inhibits glucagon secretion and induces satiation in humans with type 2 diabetes mellitus. The use of exenatide ER is safe and stimulates insulin secretion in healthy cats. Objectives The objective of this study is to assess the safety of exenatide ER and its effect on body weight, remission and metabolic control in newly diagnosed diabetic cats receiving insulin and a low-carbohydrate diet. Animals Thirty client-owned cats. Methods Prospective placebo-controlled clinical trial. Cats were treated with exenatide ER or 0.9% saline, administered SC, once weekly. Both groups received insulin glargine and a low-carbohydrate diet. Exenatide ER was administered for 16 weeks, or in cats that achieved remission it was given for 4 weeks after discontinuing insulin treatment. Nonparametric tests were used for statistical analysis. Results Cats in the exenatide ER and placebo groups had transient adverse signs including decreased appetite (60% vs. 20%, respectively, P = .06) and vomiting (53% vs. 40%, respectively, P = .715). Body weight increased significantly in the placebo group (P = .002), but not in cats receiving exenatide ER. Cats on exenatide ER achieved remission or good metabolic control in 40% or 89%, respectively, whereas in control cats percentages were 20% or 58% (P = .427 and P = .178, respectively). Conclusion and clinical importance Exenatide ER is safe in diabetic cats and does not result in weight gain. Our pilot study suggests that, should there be an additional clinically relevant beneficial effect of exenatide ER in insulin-treated cats on rate of remission and good metabolic control, it would likely approximate 20% and 30%, respectively.
- Published
- 2015
137. Preventive measures of canine leishmaniosis in Italy: Attitudes of veterinarians based on a questionnaire
- Author
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Nunzio D’Anna, Alessandra Fondati, Michele Maroli, George Lubas, Xavier Roura, Eric Zini, Saverio Paltrinieri, Lorenza Muscardin, Luigi Gradoni, and A. Zatelli
- Subjects
medicine.medical_specialty ,040301 veterinary sciences ,030231 tropical medicine ,Veterinarians ,0403 veterinary science ,03 medical and health sciences ,Dogs ,Professional Competence ,0302 clinical medicine ,Food Animals ,Dog ,medicine ,Animals ,Dog Diseases ,Leishmania infantum ,Ectoparasiticides ,Leishmaniasis ,Leishmania ,Visceral ,biology ,business.industry ,Prevention ,Diagnostic test ,04 agricultural and veterinary sciences ,biology.organism_classification ,Domperidone ,Italy ,Attitude ,Family medicine ,Leishmaniasis, Visceral ,Vaccine ,Animal Science and Zoology ,Rural area ,business ,Working environment - Abstract
There are several topical ectoparasiticides, drugs, supplements and vaccines that protect dogs against sand fly bites and/or reduce the risk of Leishmania infantum infection. This large variety of products and the absence of comparative studies mean that veterinarians are often faced with uncertainty. The aim of the study was to characterise the attitudes of veterinarians towards leishmaniosis prevention in client-owned dogs in Italy. A web-based questionnaire was prepared with 28 questions covering the working environment of veterinarians, advice given to owners regarding topical ectoparasiticides, advice on systemic prevention, and the use of diagnostic tests on which to base advice for prevention. The questionnaire was emailed to 9,426 veterinarians and answers were collected after 100 days. A total of 542 questionnaires were returned (response percentage 5.8 %): 54.8 % of the veterinarians considered their working area to be endemic, 29.3 % examined dogs from urban areas, and 42.3 % diagnosed ≥10 dogs/year with leishmaniosis. Those veterinarians who diagnosed ≥10 dogs/year with leishmaniosis were more likely to consider their working area to be endemic, whereas those who examined dogs mainly from urban areas were less likely to consider the area to be endemic. Veterinarians who considered the working area to be endemic were more prone to prescribe ectoparasiticides throughout the year, including collars and spot-on products and a combination of the two, vaccine alone or vaccine and domperidone combined, and used qualitative serological Leishmania tests. The attitude of veterinarians did not differ whether dogs were from urban or rural areas. In conclusion, veterinarians who consider their working area to be endemic or who frequently diagnose dogs with leishmaniosis tend to adopt more comprehensive strategies, and their attitudes are not influenced by the origin of the dogs: urban or rural. Overall, in Italy the attitudes of veterinarians in relation to canine leishmaniosis prevention appear to vary considerably.
- Published
- 2020
138. Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
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Barbara Contiero, Luigi Michele Coppola, Filippo Ferri, Chiara Crinò, Francesco Rossi, Neda Ranjbar Kohan, Regina Hofmann-Lehmann, Gabriele Gerardi, Daniela Enache, Carolina Callegari, Hans Lutz, Federico Porporato, Eric Zini, Marina L. Meli, University of Zurich, and Ferri, Filippo
- Subjects
Male ,0301 basic medicine ,10253 Department of Small Animals ,Feline Panleukopenia ,viruses ,lcsh:QR1-502 ,Cell Count ,lcsh:Microbiology ,0403 veterinary science ,hemic and lymphatic diseases ,Leukocytes ,Medicine ,Prospective Studies ,11434 Center for Clinical Studies ,CATS ,Leukopenia ,treatment ,biology ,virus diseases ,04 agricultural and veterinary sciences ,antiviral ,Infectious Diseases ,Oligodeoxyribonucleotides ,11404 Department of Clinical Diagnostics and Services ,Female ,medicine.symptom ,Antibody ,feline parvovirus ,040301 veterinary sciences ,CpG Oligodeoxynucleotide ,cat ,610 Medicine & health ,Viremia ,macromolecular substances ,Placebo ,Article ,03 medical and health sciences ,Virology ,Mx protein ,otorhinolaryngologic diseases ,Animals ,Survival rate ,business.industry ,2725 Infectious Diseases ,Type I interferon production ,medicine.disease ,030104 developmental biology ,Immunology ,Cats ,CpG-A ,2406 Virology ,biology.protein ,Antiviral ,Cat ,Feline parvovirus ,Treatment ,Feline Panleukopenia Virus ,business - Abstract
Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiviral response in vivo and inhibited FPV replication in vitro. The aim was to prospectively investigate the effects of CpG-A on survival, clinical score, hematological findings, antiviral response (cytokines), viremia, and fecal shedding (real-time qPCR) in cats naturally infected with FPV. Forty-two FPV-infected cats were randomized to receive 100 µ, g/kg of CpG-A (n = 22) or placebo (n = 20) subcutaneously, on admission and after 48 h. Blood and fecal samples were collected on admission, after 1, 3, and 7 days. All 22 cats showed short duration pain during CpG-A injections. The survival rate, clinical score, leukocyte and erythrocyte counts, viremia, and fecal shedding at any time-point did not differ between cats treated with CpG-A (50%) and placebo (40%). Antiviral myxovirus resistance (Mx) gene transcription increased in both groups from day 1 to 3 (p = 0.005). Antibodies against FPV on admission were associated with survival in cats (p = 0.002). In conclusion, CpG-A treatment did not improve the outcome in cats with FPV infection. FPV infection produced an antiviral response.
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- 2020
139. All cats are gray in the dark: enrichment/depletion approaches for biomarker discovery on felis catus plasma
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Daniela Pagnozzi, Maria Filippa Addis, Eric Zini, Laura Carcangiu, Silvia Tore, Sergio Uzzau, Salvatore Pisanu, University of Zurich, and Pagnozzi, D
- Subjects
0301 basic medicine ,enrichment ,10253 Department of Small Animals ,1303 Biochemistry ,Proteome ,Fibrinogen ,Biochemistry ,Chromatography, Affinity ,03 medical and health sciences ,Protein sequencing ,Tandem Mass Spectrometry ,biomarker discovery ,medicine ,1312 Molecular Biology ,Animals ,Biomarker discovery ,Shotgun proteomics ,Molecular Biology ,plasma ,biology ,630 Agriculture ,depletion ,shotgun proteomics ,Chemistry ,Ligand ,Albumin ,Blood Proteins ,030104 developmental biology ,Cats ,biology.protein ,570 Life sciences ,Apolipoprotein A1 ,Antibody ,Biomarkers ,Chromatography, Liquid ,medicine.drug - Abstract
In veterinary medicine, assay performance is often affected by the lack of species-specific diagnostic tools. Reliable biomarkers might be identified by investigating biological fluids of the species of interest, but protein sequence databases are often incomplete and human-specific devices for reducing sample complexity might fail when applied to animal plasma. Here, seven commercial methods based on different capturing agents (anti-human antibodies, affinity ligands, mixture of antibodies and ligands, and combinatorial peptide ligand libraries) are applied to cat plasma and evaluated in terms of yield, identified proteins/ peptides, and relative abundance by high-resolution shotgun proteomics and label-free quantitation. As a result, anti-human antibody-based methods are unsatisfactory. Most fail in reducing albumin and immunoglobulins, and some lead to a substantial removal of other highly abundant proteins, probably because of nonspecific interactions. A protein A/dye ligand-based method is efficient in reducing immunoglobulins, fibrinogen, and apolipoprotein A1 and A2, but not albumin, and protein identifications do not increase. Only peptide ligand libraries flatten the dynamic range, and increased protein identification (59.0%). Albumin and immunoglobulins are successfully depleted (60.7% and 35.9%, respectively). Although further studies will be required for reinforcing our observations, this work can provide a useful guide for cat plasma pretreatment in biomarker discovery studies.
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- 2018
140. Establishment of a protocol for the isolation of feline pancreatic islets
- Author
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Eric Zini, Thomas A. Lutz, I R Brandão, Claudia E Reusch, Melania Osto, University of Zurich, and Osto, Melania
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Male ,0301 basic medicine ,endocrine system ,10253 Department of Small Animals ,endocrine system diseases ,Cell Survival ,Pancreatic islets ,Experimental and Cognitive Psychology ,Tissue Culture Techniques ,Islets of Langerhans ,03 medical and health sciences ,Feline diabetes ,Behavioral Neuroscience ,2802 Behavioral Neuroscience ,medicine ,Animals ,Pancreatic duct ,geography ,CATS ,geography.geographical_feature_category ,630 Agriculture ,3205 Experimental and Cognitive Psychology ,Chemistry ,10081 Institute of Veterinary Physiology ,Islet ,Molecular biology ,Staining ,Major duodenal papilla ,030104 developmental biology ,medicine.anatomical_structure ,Cats ,Collagenase ,570 Life sciences ,biology ,Pancreas ,medicine.drug - Abstract
Diabetes mellitus is a common endocrinopathy in cats that is associated with pancreatic islets lesions. Research on isolated islets contributed to the understanding of the pathophysiology of human diabetes. Therefore, by improving the existing methods of isolation in cats, we aimed at increasing islet yield, purity and viability of feline isolated islets. Islet isolation was accomplished by pancreas perfusion with 80ml of Collagenase type IV through the pancreatic duct at the site of the major papilla. The enzymatic digestion was combined with mechanical disruption and controlled by dithizone staining. Purification was performed by filtration and handpicking. Purified islets were plated on extracellular matrix pre-coated plates and cultured for 48h. Feline islets with a high degree of viability and purity were isolated and cultured for the first time. Although the percentage of islet free from the acinar tissue relative to the total number of isolated islets was low compared to other species, the suggested protocol represents a promising progress in the procedure of islet isolation in cats.
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- 2018
141. Survival estimates and outcome predictors for shelter cats with feline panleukopenia virus infection
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Regina Hofmann-Lehmann, Paola Rocchi, Tommaso Vezzosi, Barbara Contiero, Federico Porporato, Gabriele Gerardi, Hans Lutz, Marian C. Horzinek, Edoardo Auriemma, Eric Zini, Filippo Ferri, University of Zurich, and Porporato, Federico
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,10253 Department of Small Animals ,Feline Panleukopenia ,040301 veterinary sciences ,3400 General Veterinary ,Physical examination ,Feline panleukopenia ,Animal Welfare ,Disease Outbreaks ,Cohort Studies ,0403 veterinary science ,03 medical and health sciences ,Lethargy ,chemistry.chemical_compound ,Risk Factors ,Internal medicine ,Animals ,Medicine ,Survival analysis ,Retrospective Studies ,CATS ,Leukopenia ,630 Agriculture ,General Veterinary ,biology ,medicine.diagnostic_test ,business.industry ,Retrospective cohort study ,04 agricultural and veterinary sciences ,Prognosis ,biology.organism_classification ,Survival Analysis ,030104 developmental biology ,Italy ,chemistry ,Cats ,11404 Department of Clinical Diagnostics and Services ,570 Life sciences ,Veterinary (all) ,Female ,Feline Panleukopenia Virus ,medicine.symptom ,business ,Maropitant - Abstract
OBJECTIVE To determine survival estimates and outcome predictors for shelter cats with feline panleukopenia virus (FPV) infection. DESIGN Retrospective cohort study. ANIMALS 177 shelter cats with FPV infection. PROCEDURES Medical records of cats treated for FPV infection from 2011 through 2013 were reviewed to collect information pertaining to signalment; history; results of physical examination, CBC, serum biochemical analysis, and blood gas analysis; and treatments (antimicrobials, antiparasitics, antivirals, antiemetics, analgesics, crystalloid or colloid solutions, and blood products). Survival time and outcome predictors were determined by means of Kaplan-Meier estimation, logistic regression, and mixed-model ANOVA. RESULTS Median survival time after hospital admission was 3 days; 20.3% (36/177) of cats survived to discharge from the hospital. Risk of nonsurvival was greater in cats with (vs without) signs of lethargy, rectal temperature < 37.9°C (I00.2°F), or low body weight at hospital admission. Lower (vs higher) leukocyte count on days 3,4, and 7 of hospitalization, but not at admission, was associated with nonsurvival. Amoxicillin–clavulanic acid, antiparasitics, and maropitant but not interferon-ω were associated with survival, whereas glucose infusion was associated with nonsurvival. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that FPV infection carried a poor prognosis for shelter cats. Several variables measured at admission or during hospitalization were associated with outcome. Remarkably and contrary to the existing literature, leukopenia at admission had no association with outcome, possibly owing to early prevention of complications.
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- 2018
142. Prevenzione della leishmaniosi canina: Cosa è utile sapere prima di raccomandare un prodotto topico attivo contro la puntura dei flebotomi?
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Alessandra, Fondati, Luigi, Gradoni, Lubas, George, Saverio, Paltrinieri, Xavier, Roura, Andrea, Zatelli, Eric, Zini, and Michele, Maroli
- Published
- 2018
143. Unerupted teeth in dogs and cats: a clinical, radiographic and histological study in 64 cases (2001-2017)
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Emma, Bellei, Ferro, Silvia, and Zini, Eric
- Published
- 2018
144. Left atrial anteroposterior diameter in dogs: reference interval, allometric scaling, and agreement with the left atrial-to-aortic root ratio
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MARCHESOTTI, Federica, primary, VEZZOSI, Tommaso, additional, TOGNETTI, Rosalba, additional, MARCHETTI, Francesca, additional, PATATA, Valentina, additional, CONTIERO, Barbara, additional, ZINI, Eric, additional, and DOMENECH, Oriol, additional
- Published
- 2019
- Full Text
- View/download PDF
145. Home monitoring of heart rate and heart rhythm with a smartphone-based ECG in dogs
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Vezzosi, Tommaso, primary, Tognetti, Rosalba, additional, Buralli, Carlotta, additional, Marchesotti, Federica, additional, Patata, Valentina, additional, Zini, Eric, additional, and Domenech, Oriol, additional
- Published
- 2019
- Full Text
- View/download PDF
146. Pheochromocytoma in Dogs Undergoing Adrenalectomy
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Zini, Eric, primary, Nolli, Silvia, additional, Ferri, Filippo, additional, Massari, Federico, additional, Gerardi, Gabriele, additional, Nicoli, Stefano, additional, Romanelli, Giorgio, additional, Montinaro, Vincenzo, additional, Trez, Davide, additional, Cavicchioli, Laura, additional, and Ferro, Silvia, additional
- Published
- 2018
- Full Text
- View/download PDF
147. 64-multidetector CT anatomical assessment of the feline bronchial and pulmonary vascular structures
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Panopoulos, Ioannis, primary, Auriemma, Edoardo, additional, Specchi, Swan, additional, Diana, Alessia, additional, Pietra, Marco, additional, Papastefanou, Anastasia, additional, Zini, Eric, additional, and Cipone, Mario, additional
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- 2018
- Full Text
- View/download PDF
148. Comparison of doxorubicin-cyclophosphamide with doxorubicin-dacarbazine for the adjuvant treatment of canine hemangiosarcoma
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Laura Marconato, Damiano Stefanello, Eric Zini, and Riccardo Finotello
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Oncology ,Chemotherapy ,medicine.medical_specialty ,General Veterinary ,Cyclophosphamide ,040301 veterinary sciences ,business.industry ,Dacarbazine ,medicine.medical_treatment ,04 agricultural and veterinary sciences ,medicine.disease ,Canine Hemangiosarcoma ,Surgery ,0403 veterinary science ,03 medical and health sciences ,Regimen ,0302 clinical medicine ,Hemangiosarcoma ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Doxorubicin ,business ,Adjuvant ,medicine.drug - Abstract
Canine hemangiosarcoma (HSA) is a neoplasm of vascular endothelial origin that has an aggressive biological behaviour, with less than 10% of dogs alive at 12-months postdiagnosis. Treatment of choice consists of surgery followed by adjuvant doxorubicin-based chemotherapy. We prospectively compared adjuvant doxorubicin and dacarbazine (ADTIC) to a traditional doxorubicin and cyclophosphamide (AC) treatment, aiming at determining safety and assessing whether this regimen prolongs survival and time to metastasis (TTM). Twenty-seven dogs were enrolled; following staging work-up, 18 were treated with AC and 9 with ADTIC. Median TTM and survival time were longer for dogs treated with ADTIC compared with those receiving AC (>550 versus 112 days, P = 0.021 and >550 versus 142 days, P = 0.011, respectively). Both protocols were well tolerated, without need for dose reduction or increased interval between treatments. A protocol consisting of combined doxorubicin and dacarbazine is safe in dogs with HSA and prolongs TTM and survival time.
- Published
- 2015
149. EVALUATION OF THE COMPUTED TOMOGRAPHIC 'SENTINEL CLOT SIGN' TO IDENTIFY BLEEDING ABDOMINAL ORGANS IN DOGS WITH HEMOABDOMEN
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Specchi, Swan, Auriemma, Edoardo, Morabito, Simona, Ferri, Filippo, Zini, Eric, Piola, Valentina, Pey, Pascaline, Rossi, Federica, Specchi, Swan, Auriemma, Edoardo, Morabito, Simona, Ferri, Filippo, Zini, Eric, Piola, Valentina, Pey, Pascaline, and Rossi, Federica
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small animal ,Male ,Hematoma ,small animals ,Animal ,sentinel clot sign ,canine ,X-Ray Computed ,Dogs ,CT ,spleen ,Animals ,Dog Diseases ,Female ,Italy ,Prevalence ,Retrospective Studies ,Tomography, X-Ray Computed ,Veterinary (all) ,Retrospective Studie ,Dog ,Dog Disease ,Tomography - Abstract
The CT âsentinel clot signâ has been defined as the highest attenuation hematoma adjacent to a bleeding organ in humans with hemoabdomen. The aims of this retrospective descriptive multicenter study were to describe CT findings in a sample of dogs with surgically or necropsy confirmed intra-abdominal bleeding and determine prevalence of the âsentinel clot signâ adjacent to the location of bleeding. Medical records between 2012 and 2014 were searched for dogs with hemoabdomen and in which the origin of the bleeding was confirmed either with surgery or necropsy. Retrieved CT images were reviewed for the presence and localization of the âsentinel clot sign,â HU measurements of the âsentinel clot signâ and hemoabdomen, and presence of extravasation of contrast media within the abdominal cavity. Nineteen dogs were included. Three dogs were excluded due to the low amount of blood that did not allow the identification of a âsentinel clot sign.â A âsentinel clot signâ was detected in the proximity of the confirmed bleeding organ in 14/16 (88%) of the patients. The mean HU of the âsentinel clot signâ was 56 (range: 43â70) while that of the hemoabdomen was 34 (range: 20â45). Active hemorrhage was identified as extravasation of contrast medium within the peritoneal cavity from the bleeding organ in three dogs. In conclusion, the CT âsentinel clot signâ may be helpful for identifying the source of bleeding in dogs with hemoabdomen.
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- 2016
150. Exocrine Pancreas in Cats with Diabetes Mellitus
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Melania Osto, Silvia Ferro, Eric Zini, Thomas A. Lutz, Claudia E Reusch, Laura Cavicchioli, Luigi Michele Coppola, Rossella Zanetti, R S Heller, F Lunardi, Franco Guscetti, University of Zurich, and Zini, Eric
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Male ,medicine.medical_specialty ,10253 Department of Small Animals ,acinar cells ,040301 veterinary sciences ,3400 General Veterinary ,medicine.medical_treatment ,10184 Institute of Veterinary Pathology ,cat ,030209 endocrinology & metabolism ,Cat Diseases ,Glucagon ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Internal medicine ,medicine ,Animals ,Insulin ,pancreas ,CATS ,630 Agriculture ,General Veterinary ,business.industry ,Pancreatic islets ,diabetes mellitus ,inflammation ,Ketosis ,04 agricultural and veterinary sciences ,10081 Institute of Veterinary Physiology ,medicine.disease ,Pancreas, Exocrine ,Ketoacidosis ,medicine.anatomical_structure ,Endocrinology ,Pancreatitis ,Cats ,570 Life sciences ,biology ,Female ,Pancreas ,business - Abstract
Pancreatitis has been described in cats with diabetes mellitus, although the number of studies currently available is very limited. In addition, ketoacidosis has been hypothesized to be associated with pancreatitis in diabetic cats. The aims of the present study were to investigate whether diabetic cats have pancreatitis and to determine if pancreatitis is more frequent with ketoacidosis. Samples of pancreas were collected postmortem from 37 diabetic cats, including 15 with ketoacidosis, and 20 control cats matched for age, sex, breed, and body weight. Sections were stained with hematoxylin and eosin, double-labeled for insulin/CD3, insulin/CD20, insulin/myeloperoxidase, insulin/PCNA, and glucagon/Ki67, and single-labeled for Iba1. A previously proposed semiquantitative score was used to characterize pancreatitis, along with counts of inflammatory cells. Scores of pancreatitis and the number of neutrophils, macrophages, and lymphocytes in the exocrine pancreas did not differ between diabetic and control cats or between diabetic cats with and without ketoacidosis. Of note, PCNA-positive acinar cells were increased ( P = .002) in diabetic cats, particularly near islets ( P < .001). Ki67-positive acinar cells were increased only near islets ( P = .038). Ketoacidosis was not linked to proliferation. The results suggest that histopathologic evidence of pancreatitis may not be more frequent in diabetic cats and that ketoacidosis may not be associated with it at the time of death. Augmented PCNA-positive acinar cells might indicate increased proliferation due to chronic pancreatitis. The reason behind the prevalent proliferation of acinar cells surrounding pancreatic islets deserves further investigation.
- Published
- 2016
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