151. Prognostic significance of miR-181b and miR-21 in gastric cancer patients treated with S-1/Oxaliplatin or Doxifluridine/Oxaliplatin
- Author
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Xiao Zheng, Binfeng Lu, Changping Wu, Jingting Jiang, Xueguang Zhang, Qi Zhou, Jingfang Ju, Haijiao Yan, and Xiao Xu
- Subjects
Male ,Organoplatinum Compounds ,lcsh:Medicine ,Gene Expression ,Kaplan-Meier Estimate ,Pharmacology ,Gastroenterology ,Floxuridine ,Antineoplastic Combined Chemotherapy Protocols ,Molecular Cell Biology ,Gastrointestinal Cancers ,Pathology ,Medicine ,lcsh:Science ,Stomach and Duodenum ,Multidisciplinary ,Hazard ratio ,Middle Aged ,Prognosis ,Gene Expression Regulation, Neoplastic ,Oxaliplatin ,Drug Combinations ,Treatment Outcome ,Oncology ,Gastritis ,Female ,medicine.drug ,Research Article ,medicine.medical_specialty ,Antineoplastic Agents ,Gastroenterology and Hepatology ,Tegafur ,Molecular Genetics ,Stomach Neoplasms ,Diagnostic Medicine ,Internal medicine ,Gastrointestinal Tumors ,Genetics ,Humans ,Biology ,Survival analysis ,business.industry ,Gene Expression Profiling ,lcsh:R ,Cancer ,Computational Biology ,Cancers and Neoplasms ,medicine.disease ,digestive system diseases ,Log-rank test ,Regimen ,MicroRNAs ,Oxonic Acid ,Gastric Cancer ,lcsh:Q ,business ,Biomarkers ,General Pathology - Abstract
Background The goal of this study is to evaluate the effectiveness of S-1/Oxaliplatin vs. Doxifluridine/Oxaliplatin regimen and to identify miRNAs as potential prognostic biomarkers in gastric cancer patients. The expression of candidate miRNAs was quantified from fifty-five late stage gastric cancer FFPE specimens. Experimental Design Gastric cancer patients with KPS>70 were recruited for the trial. The control group was treated with 400 mg/twice/day Doxifluridine plus i.v. with Oxaliplatin at 130 mg/m2/first day/4 week cycle. The testing group was treated with S-1 at 40 mg/twice/day/4 week cycle plus i.v. with Oxaliplatin at 130 mg/m2/first day/4 week cycle. Total RNAs were extracted from normal and gastric tumor specimens. The levels of miRNAs were quantified using real time qRT-PCR expression analysis. Results The overall objective response rate (CR+PR) of patients treated with S-1/Oxaliplatin was 33.3% (CR+PR) vs. 17.6% (CR+PR) with Doxifluridine/Oxaliplatin for advanced stage gastric cancer patients. The average overall survival for patients treated with S-1/Oxaliplatin was 7.80 month vs. 7.30 month with patients treated with Doxifluridine/Oxaliplatin. The expression of miR-181b (P = 0.022) and miR-21 (P = 0.0029) was significantly overexpressed in gastric tumors compared to normal gastric tissues. Kaplan-Meier survival analysis revealed that low levels of miR-21 expression (Log rank test, hazard ratio: 0.17, CI = 0.06–0.45; P = 0.0004) and miR-181b (Log rank test, hazard ratio: 0.37, CI = 0.16–0.87; P = 0.018) are closely associated with better patient's overall survival for both S-1 and Doxifluridine based regimens. Conclusion Patients treated with S-1/Oxaliplatin had a better response than those treated with Doxifluridine/Oxaliplatin. miR-21 and miR-181b hold great potential as prognostic biomarkers in late stage gastric cancer.
- Published
- 2011