345 results on '"Guo-Ping Shi"'
Search Results
152. The Immunomodulatory Action of Sialostatin L on Dendritic Cells Reveals Its Potential to Interfere with Autoimmunity
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Anderson Sá-Nunes, Guo-Ping Shi, John F. Andersen, Michalis Kotsyfakis, José M. C. Ribeiro, André Báfica, Lis Ribeiro do Valle Antonelli, Eun-Young Choi, Ivo M.B. Francischetti, and Triantafyllos Chavakis
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Cathepsin ,Autoimmune disease ,T cell ,Cellular differentiation ,Immunology ,Experimental autoimmune encephalomyelitis ,Dendritic cell ,Biology ,medicine.disease ,medicine.disease_cause ,Cell biology ,Autoimmunity ,medicine.anatomical_structure ,Antigen ,medicine ,Immunology and Allergy - Abstract
Sialostatin L (SialoL) is a secreted cysteine protease inhibitor identified in the salivary glands of the Lyme disease vector Ixodes scapularis. In this study, we reveal the mechanisms of SialoL immunomodulatory actions on the vertebrate host. LPS-induced maturation of dendritic cells from C57BL/6 mice was significantly reduced in the presence of SialoL. Although OVA degradation was not affected by the presence of SialoL in dendritic cell cultures, cathepsin S activity was partially inhibited, leading to an accumulation of a 10-kDa invariant chain intermediate in these cells. As a consequence, in vitro Ag-specific CD4+ T cell proliferation was inhibited in a time-dependent manner by SialoL, and further studies engaging cathepsin S−/− or cathepsin L−/− dendritic cells confirmed that the immunomodulatory actions of SialoL are mediated by inhibition of cathepsin S. Moreover, mice treated with SialoL displayed decreased early T cell expansion and recall response upon antigenic stimulation. Finally, SialoL administration during the immunization phase of experimental autoimmune encephalomyelitis in mice significantly prevented disease symptoms, which was associated with impaired IFN-γ and IL-17 production and specific T cell proliferation. These results illuminate the dual mechanism by which a human disease vector protein modulates vertebrate host immunity and reveals its potential in prevention of an autoimmune disease.
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- 2009
153. Critical Role of Mast Cell Chymase in Mouse Abdominal Aortic Aneurysm Formation
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Jiusong Sun, Gunnar Pejler, Guo-Ping Shi, Peter Libby, Jian Liu, Michael F. Gurish, Robert W. Thompson, Jes S. Lindholt, Jie Zhang, Richard L. Stevens, Galina K. Sukhova, Terri L. Ennis, Magnus Åbrink, and Aina He
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Male ,Pathology ,Apoptosis ,030204 cardiovascular system & hematology ,environment and public health ,Muscle, Smooth, Vascular ,Pathogenesis ,Aortic aneurysm ,Mice ,0302 clinical medicine ,Mast Cells ,Aged, 80 and over ,0303 health sciences ,biology ,Serine Endopeptidases ,Middle Aged ,Mast cell ,Abdominal aortic aneurysm ,medicine.anatomical_structure ,cardiovascular system ,Female ,Cardiology and Cardiovascular Medicine ,Adult ,medicine.medical_specialty ,macromolecular substances ,Gene Expression Regulation, Enzymologic ,Article ,03 medical and health sciences ,Aneurysm ,Chymases ,Physiology (medical) ,medicine ,Animals ,Humans ,cardiovascular diseases ,030304 developmental biology ,Aged ,Cathepsin ,Serine protease ,business.industry ,Macrophages ,Microcirculation ,Chymase ,medicine.disease ,Cathepsins ,Mice, Mutant Strains ,Mice, Inbred C57BL ,enzymes and coenzymes (carbohydrates) ,Disease Models, Animal ,biology.protein ,business ,Aortic Aneurysm, Abdominal - Abstract
Background—Mast cell chymase may participate in the pathogenesis of human abdominal aortic aneurysm (AAA), yet a direct contribution of this serine protease to AAA formation remains unknown.Methods and Results—Human AAA lesions had high numbers of chymase-immunoreactive mast cells. Serum chymase level correlated with AAA growth rate (P=0.009) in a prospective clinical study. In experimental AAA produced by aortic elastase perfusion in wild-type (WT) mice or those deficient in the chymase ortholog mouse mast cell protease-4 (mMCP-4) or deficient in mMCP-5 (Mcpt4−/−,Mcpt5−/−),Mcpt4−/−but notMcpt5−/−had reduced AAA formation 14 days after elastase perfusion. Even 8 weeks after perfusion, aortic expansion inMcpt4−/−mice fell by 50% compared with that of the WT mice (P=0.0003). AAA lesions inMcpt4−/−mice had fewer inflammatory cells and less apoptosis, angiogenesis, and elastin fragmentation than those of WT mice. AlthoughKitW-sh/W-shmice had protection from AAA formation, reconstitution with mast cells from WT mice, but not those fromMcpt4−/−mice, partially restored the AAA phenotype. Mechanistic studies suggested that mMCP-4 regulates expression and activation of cysteine protease cathepsins, elastin degradation, angiogenesis, and vascular cell apoptosis.Conclusions—High chymase-positive mast cell content in human AAA lesions, greatly reduced AAA formation inMcpt4−/−mice, and significant correlation of serum chymase levels with human AAA expansion rate suggests participation of mast cell chymase in the progression of human and mouse AAA.
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- 2009
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154. Leukocyte cathepsin S is a potent regulator of both cell and matrix turnover in advanced atherosclerosis
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E. E. van der Wall, Th.J.C. van Berkel, R de Nooijer, P.J. van Santbrink, R. Dorland, Marijke M. Westra, Guo-Ping Shi, J.H. von der Thüsen, E.A.L. Biessen, Herman S. Overkleeft, A.O. Kraaijeveld, S.H. van Heiningen, Michiel A. Leeuwenburgh, Joop Jukema, Petri T. Kovanen, Ilze Bot, ACS - Amsterdam Cardiovascular Sciences, Pathology, and Faculteit der Geneeskunde
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Proteases ,Pathology ,medicine.medical_specialty ,Myocytes, Smooth Muscle ,Apoptosis ,030204 cardiovascular system & hematology ,Lesion ,Mice ,Necrosis ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Leukocytes ,medicine ,Animals ,Protease Inhibitors ,Aorta ,Cells, Cultured ,Bone Marrow Transplantation ,Cell Proliferation ,030304 developmental biology ,Cathepsin S ,Mice, Knockout ,Cathepsin ,Transplantation Chimera ,0303 health sciences ,CATS ,biology ,Chemistry ,Atherosclerosis ,Elastic Tissue ,Cathepsins ,Molecular biology ,Extracellular Matrix ,3. Good health ,Transplantation ,Fibronectin ,Disease Models, Animal ,Cholesterol ,Receptors, LDL ,Macrophages, Peritoneal ,biology.protein ,Diet, Atherogenic ,Female ,Collagen ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Foam Cells - Abstract
Objective— A dysbalance of proteases and their inhibitors is instrumental in remodeling of atherosclerotic plaques. One of the proteases implicated in matrix degradation is cathepsin-S (CatS). To address its role in advanced lesion composition, we generated chimeric LDLr −/− mice deficient in leukocyte CatS by transplantation with CatS −/− ×LDLr −/− or with LDLr −/− bone marrow and administered a high-fat diet. Methods and Results— No difference in aortic root lesion size could be detected between CatS +/+ and CatS −/− chimeras. However, leukocyte CatS deficiency markedly changed plaque morphology and led to a dramatic reduction in necrotic core area by 77% and an abundance of large foam cells. Plaques of CatS −/− chimeras contained 17% more macrophages, 62% less SMCs, and 33% less intimal collagen. The latter two could be explained by a reduced number of elastic lamina fractures. Moreover, macrophage apoptosis was reduced by 60% with CatS deficiency. In vitro, CatS was found to be involved in cholesterol metabolism and in macrophage apoptosis in a collagen and fibronectin matrix. Conclusion— Leukocyte CatS deficiency results in considerably altered plaque morphology, with smaller necrotic cores, reduced apoptosis, and decreased SMC content and collagen deposition and may thus be critical in plaque stability.
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- 2009
155. Deficiency and Inhibition of Cathepsin K Reduce Body Weight Gain and Increase Glucose Metabolism in Mice
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Karine Clément, Guo-Ping Shi, Min Yang, Jian Liu, Jie Zhang, Jiusong Sun, Michael A. Shi, Froogh Darakhshan, Gregory Dolgnov, Michèle Guerre-Millo, Bruce D. Gelb, and Tinghu Zhang
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Blood Glucose ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Cathepsin K ,Gene Expression ,Mice, Obese ,Adipose tissue ,Cysteine Proteinase Inhibitors ,Carbohydrate metabolism ,Biology ,Weight Gain ,Article ,Mice ,3T3-L1 Cells ,Internal medicine ,Adipocytes ,medicine ,Animals ,Humans ,Insulin ,Cells, Cultured ,Metabolic Syndrome ,Mice, Knockout ,Cathepsin ,Adipogenesis ,Cathepsins ,Fibronectins ,Mice, Inbred C57BL ,Fibronectin ,Glucose ,Endocrinology ,Schizophrenia ,biology.protein ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Weight gain ,Antipsychotic Agents - Abstract
Objectives— Previous studies demonstrated increased levels of cysteine proteases cathepsins in serum and adipose tissues from obese patients. We now provide evidence from a mouse model of obesity to suggest a direct participation of cathepsin K (CatK) in mouse body weight gain and glucose metabolism. Methods and Results— Using real-time polymerase chain reaction, we detected 12-fold increase in CatK transcripts after adipogenesis of human preadipocytes. Using an immunohistology analysis, we consistently observed high levels of CatK expression in adipose tissues from obese humans and mice. Selective inhibition of CatK activity blocked the lipid accumulation in human and mouse preadipocytes. In mice, CatK deficiency reduced significantly diet-induced body weight gain and serum glucose and insulin levels. Similar results were obtained in diet-induced and genetically created ( ob/ob ) obese mice after animals were treated with a CatK-selective inhibitor. Mechanistic study demonstrated a role for CatK in degrading fibronectin, a matrix protein that controls adipogenesis. Deficiency or inhibition of CatK leads to fibronectin accumulation in muscle and adipose tissues. Conclusion— This study demonstrates an essential role of CatK in adipogenesis and mouse body weight gain, possibly via degradation of fibronectin, thus suggesting a novel therapeutic strategy for the control of obesity by regulating CatK activity.
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- 2008
156. Matrix Metalloproteinase-14 Deficiency in Bone Marrow–Derived Cells Promotes Collagen Accumulation in Mouse Atherosclerotic Plaques
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Peter Libby, Fabrice Schneider, James P. Canner, Norbert Gerdes, Sai Man Timothy Tang, Guo-Ping Shi, Galina K. Sukhova, Masanori Aikawa, and Suneel S. Apte
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medicine.medical_specialty ,Pathology ,Myocytes, Smooth Muscle ,Neovascularization, Physiologic ,Bone Marrow Cells ,Matrix metalloproteinase ,Mice ,Mice, Congenic ,Cell Movement ,In vivo ,Physiology (medical) ,Internal medicine ,Matrix Metalloproteinase 13 ,Matrix Metalloproteinase 14 ,medicine ,Animals ,Myocyte ,Macrophage ,Aorta ,Bone Marrow Transplantation ,Mice, Knockout ,Enzyme Precursors ,Tumor Necrosis Factor-alpha ,business.industry ,Macrophages ,Atherosclerosis ,Extracellular Matrix ,Enzyme Activation ,Mice, Inbred C57BL ,Cholesterol ,medicine.anatomical_structure ,Endocrinology ,Receptors, LDL ,Radiation Chimera ,Diet, Atherogenic ,MMP14 ,Interstitial collagenase ,Collagen ,Bone marrow ,Cardiology and Cardiovascular Medicine ,business ,Lipoprotein - Abstract
Background— Interstitial collagen plays a crucial structural role in arteries. Although in vitro results suggest collagenase activity for membrane-bound matrix metalloproteinase type 1 (MMP-14), in vivo evidence for such a function in atherosclerosis remains scant. Methods and Results— Because Mmp14 −/− mice die by 3 weeks of age, this study used lethally irradiated low-density lipoprotein receptor–deficient mice reconstituted with syngeneic bone marrow cells of Mmp14 −/− or Mmp14 +/+ mice. In both groups, histological analyses of the aortic root revealed similar plaque size and macrophage and smooth muscle cell content after 8 or 16 weeks of atherogenic diet. By 16 weeks, however, the plaques of low-density lipoprotein receptor–deficient mice engrafted with Mmp14 −/− bone marrow (n=12) contained significantly more interstitial collagen than those receiving Mmp14 +/+ bone marrow (n=14; P Mmp14 −/− mice had significantly less interstitial collagenase activity than those from Mmp14 +/+ mice both basally ( P P Conclusion— MMP-14 from bone marrow–derived cells can influence the collagen content of mouse atheroma, a critical component of plaque stability.
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- 2008
157. Cathepsin L activity controls adipogenesis and glucose tolerance
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Min Yang, Yaou Zhang, Galina K. Sukhova, Alessandro Doria, Jiusong Sun, Jian Liu, Peter Libby, Barbara B. Kahn, Michèle Guerre-Millo, Guo-Ping Shi, Jie-Hong Pan, Odile D. Peroni, Nobuhiko Katunuma, and Karine Clément
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Male ,medicine.medical_specialty ,Pyridines ,Cathepsin L ,medicine.medical_treatment ,Glucose uptake ,Mice, Obese ,Carbohydrate metabolism ,Receptor, IGF Type 1 ,Mice ,Internal medicine ,Glucose Intolerance ,Adipocytes ,CCAAT-Enhancer-Binding Protein-alpha ,medicine ,Animals ,Humans ,Receptor ,Cells, Cultured ,Mice, Knockout ,Adipogenesis ,biology ,Insulin ,Body Weight ,Glucose transporter ,Cell Differentiation ,Cell Biology ,Cathepsins ,Receptor, Insulin ,Fibronectins ,Mice, Inbred C57BL ,PPAR gamma ,Cysteine Endopeptidases ,Insulin receptor ,Glucose ,Endocrinology ,biology.protein ,Epoxy Compounds - Abstract
Cysteine proteases play an important part in human pathobiology. This report shows the participation of cathepsin L (CatL) in adipogenesis and glucose intolerance. In vitro studies demonstrate the role of CatL in the degradation of the matrix protein fibronectin, insulin receptor (IR) and insulin-like growth factor-1 receptor (IGF-1R), essential molecules for adipogenesis and glucose metabolism. CatL inhibition leads to the reduction of human and murine pre-adipocyte adipogenesis or lipid accumulation, protection of fibronectin from degradation, accumulation of IR and IGF-1R beta-subunits, and an increase in glucose uptake. CatL-deficient mice are lean and have reduced levels of serum glucose and insulin but increased levels of muscle IR beta-subunits, fibronectin and glucose transporter (Glut)-4, although food/water intake and energy expenditure of these mice are no less than their wild-type littermates. Importantly, the pharmacological inhibition of CatL also demonstrates reduced body weight gain and serum insulin levels, and increased glucose tolerance, probably due to increased levels of muscle IR beta-subunits, fibronectin and Glut-4 in both diet-induced obese mice and ob/ob mice. Increased levels of CatL in obese and diabetic patients suggest that this protease is a novel target for these metabolic disorders.
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- 2007
158. Mast cells promote atherosclerosis by releasing proinflammatory cytokines
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Guo-Ping Shi, Paul J. Wolters, Shiro Kitamoto, Min Yang, Peter Libby, Jon Mallen-St. Clair, Jiusong Sun, Galina K. Sukhova, and Lindsey A MacFarlane
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Male ,Adoptive cell transfer ,Mice, Transgenic ,General Biochemistry, Genetics and Molecular Biology ,Proinflammatory cytokine ,Mice ,Animals ,Humans ,Medicine ,Macrophage ,Mast Cells ,Interleukin 5 ,Mice, Knockout ,Innate immune system ,business.industry ,General Medicine ,Atherosclerosis ,Mast cell ,Mice, Inbred C57BL ,Interleukin 33 ,medicine.anatomical_structure ,Immunology ,Cancer research ,Cytokines ,Tumor necrosis factor alpha ,Inflammation Mediators ,business - Abstract
Mast cells contribute importantly to allergic and innate immune responses by releasing various preformed and newly synthesized mediators. Previous studies have shown mast cell accumulation in human atherosclerotic lesions. This report establishes the direct participation of mast cells in atherogenesis in low-density lipoprotein receptor-deficient (Ldlr(-/-)) mice. Atheromata from compound mutant Ldlr(-/-) Kit(W-sh)(/W-sh) mice showed decreased lesion size, lipid deposition, T-cell and macrophage numbers, cell proliferation and apoptosis, but increased collagen content and fibrous cap development. In vivo, adoptive transfer of syngeneic wild-type or tumor necrosis factor (TNF)-alpha-deficient mast cells restored atherogenesis to Ldlr(-/-)Kit(W-sh/W-sh) mice. Notably, neither interleukin (IL)-6- nor interferon (IFN)-gamma-deficient mast cells did so, indicating that the inhibition of atherogenesis in Ldlr(-/-)Kit(W-sh/W-sh) mice resulted from the absence of mast cells and mast cell-derived IL-6 and IFN-gamma. Compared with wild-type or TNF-alpha-deficient mast cells, those lacking IL-6 or IFN-gamma did not induce expression of proatherogenic cysteine proteinase cathepsins from vascular cells in vitro or affect cathepsin and matrix metalloproteinase activities in atherosclerotic lesions, implying that mast cell-derived IL-6 and IFN-gamma promote atherogenesis by augmenting the expression of matrix-degrading proteases. These observations establish direct participation of mast cells and mast cell-derived IL-6 and IFN-gamma in mouse atherogenesis and provide new mechanistic insight into the pathogenesis of this common disease.
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- 2007
159. Optical Visualization of Cathepsin K Activity in Atherosclerosis With a Novel, Protease-Activatable Fluorescence Sensor
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Dong-Eog Kim, Elena Aikawa, Ashvin N. Pande, Rainer H. Kohler, Peter Libby, Ralph Weissleder, Luisa Quinti, Farouc A. Jaffer, Guo-Ping Shi, and Ching-Hsuan Tung
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Carotid Artery Diseases ,medicine.medical_treatment ,Cathepsin K ,Matrix metalloproteinase ,Biology ,Mice ,Apolipoproteins E ,In vivo ,Physiology (medical) ,Fluorescence microscope ,medicine ,Animals ,Humans ,Fluorescent Dyes ,Spectroscopy, Near-Infrared ,Protease ,Macrophages ,Cathepsins ,Immunohistochemistry ,Cysteine protease ,Mice, Mutant Strains ,Imaging agent ,Elastin ,Enzyme Activation ,Carotid Arteries ,Microscopy, Fluorescence ,Biochemistry ,Cardiology and Cardiovascular Medicine ,Fluorescein-5-isothiocyanate ,Ex vivo ,Peptide Hydrolases - Abstract
Background— Cathepsin K (CatK), a potent elastinolytic and collagenolytic cysteine protease, likely participates in the evolution and destabilization of atherosclerotic plaques. To assess better the biology of CatK activity in vivo, we developed a novel near-infrared fluorescence (NIRF) probe for imaging of CatK and evaluated it in mouse and human atherosclerosis. Methods and Results— The NIRF imaging agent consists of the CatK peptide substrate GHPGGPQGKC-NH 2 linked to an activatable fluorogenic polymer. In vitro, CatK produced a 2- to 14-fold activation of the agent over other cysteine and matrix metalloproteinases ( P 8-fold activation over a control imaging agent ( P 100% NIRF signal increases in apolipoprotein E −/− mice in vivo (n=13; P P Conclusions— Use of this novel protease-activatable NIRF agent for optical imaging in vivo demonstrated preferential localization of enzymatically active CatK to macrophages, consistent with their known greater elastinolytic capabilities compared with smooth muscle cells. Augmented CatK proteolysis in atheromata further links CatK to vascular remodeling and plaque vulnerability.
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- 2007
160. Cathepsin L Deficiency Reduces Diet-Induced Atherosclerosis in Low-Density Lipoprotein Receptor–Knockout Mice
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Christoph Peters, Victoria A. Love, Paurene Duramad, Shiro Kitamoto, Peter Libby, Yadong Zhang, Galina K. Sukhova, Guo-Ping Shi, Chongxiu Sun, Min Yang, Jiusong Sun, and Xiuwei Yang
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Male ,Cathepsin L ,Aorta, Thoracic ,Biology ,Monocytes ,Muscle, Smooth, Vascular ,Extracellular matrix ,Mice ,Physiology (medical) ,Animals ,Receptor ,Cells, Cultured ,Mice, Knockout ,Cathepsin ,Chemotaxis ,Endothelial Cells ,Atherosclerosis ,Cathepsins ,Lipids ,Molecular biology ,Cysteine Endopeptidases ,Receptors, LDL ,Biochemistry ,Knockout mouse ,LDL receptor ,Disease Progression ,biology.protein ,Diet, Atherogenic ,Cardiology and Cardiovascular Medicine ,Elastin ,Lipoprotein - Abstract
Background—Remodeling of the arterial extracellular matrix participates importantly in atherogenesis and plaque complication. Increased expression of the elastinolytic and collagenolytic enzyme cathepsin L (Cat L) in human atherosclerotic lesions suggests its participation in these processes, a hypothesis tested here in mice.Methods and Results—We generated Cat L and low-density lipoprotein receptor (LDLr) double-deficient (LDLr−/−Cat L−/−) mice by crossbreeding Cat L–null (Cat L−/−) and LDLr-deficient (LDLr−/−) mice. After 12 and 26 weeks of a Western diet, LDLr−/−Cat L−/−mice had significantly smaller atherosclerotic lesions and lipid cores compared with littermate control LDLr−/−Cat L+/−and LDLr−/−Cat L+/+mice. In addition, lesions from the compound mutant mice showed significantly reduced levels of collagen, medial elastin degradation, CD4+T cells, macrophages, and smooth muscle cells. Mechanistic studies showed that Cat L contributes to the degradation of extracellular matrix elastin and collagen by aortic smooth muscle cells. Smooth muscle cells from LDLr−/−Cat L−/−mice or those treated with a Cat L–selective inhibitor demonstrated significantly less degradation of elastin and collagen and delayed transmigration through elastin in vitro. Cat L deficiency also significantly impaired monocyte and T-lymphocyte transmigration through a collagen matrix in vitro, suggesting that blood-borne leukocyte penetration through the arterial basement membrane requires Cat L. Cysteine protease active site labeling demonstrated that Cat L deficiency did not affect the activity of other atherosclerosis-associated cathepsins in aortic smooth muscle cells and monocytes.Conclusions—Cat L directly participates in atherosclerosis by degrading elastin and collagen and regulates blood-borne leukocyte transmigration and lesion progression.
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- 2007
161. Expression of cathepsin K is regulated by shear stress in cultured endothelial cells and is increased in endothelium in human atherosclerosis
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Hanjoong Jo, Randall F. Ankeny, Daiana Weiss, Guo-Ping Shi, Manu O. Platt, J. D. Vega, and W. R. Taylor
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Proteases ,Endothelium ,Physiology ,Cathepsin K ,Aorta, Thoracic ,Biology ,Extracellular matrix ,Mice ,Physiology (medical) ,medicine ,Animals ,Humans ,RNA, Messenger ,Cells, Cultured ,Cathepsin S ,Cathepsin ,Atherosclerosis ,Cathepsins ,In vitro ,Cell biology ,Mice, Inbred C57BL ,Endothelial stem cell ,Disease Models, Animal ,medicine.anatomical_structure ,Biochemistry ,Endothelium, Vascular ,Stress, Mechanical ,Cardiology and Cardiovascular Medicine - Abstract
Cathepsins, the lysosomal cysteine proteases, are involved in vascular remodeling and atherosclerosis. Genetic knockout of cathepsins S and K in mice has shown to reduce atherosclerosis, although the molecular mechanisms remain unclear. Because atherosclerosis preferentially occurs in arteries exposed to disturbed flow conditions, we hypothesized that shear stress would regulate cathepsin K expression and activity in endothelial cells. Mouse aortic endothelial cells (MAEC) exposed to proatherogenic oscillatory shear (OS, ± 5 dyn/cm2for 1 day) showed significantly higher cathepsin K expression and activity than that of atheroprotective, unidirectional laminar shear stress (LS, 15 dyn/cm2for 1 day). Western blot and active-site labeling studies showed an active, mature form of cathepsin K in the conditioned medium of MAEC exposed to OS but not in that of LS. Functionally, MAEC exposed to OS significantly increased elastase and gelatinase activity above that of LS. The OS-dependent elastase and gelatinase activities were significantly reduced by knocking down cathepsin K with small-interfering (si) RNA, but not by a nonsilencing siRNA control, suggesting that cathepsin K is a shear-sensitive protease. In addition, immunohistochemical analysis of atherosclerotic human coronary arteries showed a positive correlation between the cathepsin K expression levels in endothelium and elastic lamina integrity. These findings suggest that cathepsin K is a mechanosensitive, extracellular matrix protease that, in turn, may be involved in arterial wall remodeling and atherosclerosis.
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- 2007
162. Asthma Associates With Human Abdominal Aortic Aneurysm and Rupture
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Alan Daugherty, Xiaozhu Huang, Cleverson Rodrigues Fernandes, Peter Libby, Holger Wemmelund, Xiang Cheng, Galina K. Sukhova, Cong-Lin Liu, Jinying Zhang, Jes S. Lindholt, Mengyang Liao, Henrik Vestergaard, Bruce D. Levy, Guo-Ping Shi, Søren Paaske Johnsen, Chongzhe Yang, and Yi Wang
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0301 basic medicine ,Male ,Time Factors ,Databases, Factual ,Denmark ,030204 cardiovascular system & hematology ,law.invention ,0302 clinical medicine ,Randomized controlled trial ,law ,Risk Factors ,Odds Ratio ,Anti-Asthmatic Agents ,Registries ,Aged, 80 and over ,education.field_of_study ,Middle Aged ,Abdominal aortic aneurysm ,Bronchodilator Agents ,risk factor ,Randomized Controlled Trial ,Female ,Cardiology and Cardiovascular Medicine ,Risk assessment ,medicine.medical_specialty ,Aortic Rupture ,Population ,Observational Study ,Risk Assessment ,Article ,03 medical and health sciences ,abdominal aortic aneurysm ,Research Support, N.I.H., Extramural ,Internal medicine ,medicine ,Journal Article ,Humans ,Risk factor ,education ,Aortic rupture ,Asthma ,Aged ,business.industry ,Odds ratio ,asthma ,medicine.disease ,Surgery ,030104 developmental biology ,Logistic Models ,inflammation ,business ,Aortic Aneurysm, Abdominal - Abstract
Objective— Both asthma and abdominal aortic aneurysms (AAA) involve inflammation. It remains unknown whether these diseases interact. Approach and Results— Databases analyzed included Danish National Registry of Patients, a population-based nationwide case–control study included all patients with ruptured AAA and age- and sex-matched AAA controls without rupture in Denmark from 1996 to 2012; Viborg vascular trial, subgroup study of participants from the population-based randomized Viborg vascular screening trial. Patients with asthma were categorized by hospital diagnosis, bronchodilator use, and the recorded use of other anti-asthma prescription medications. Logistic regression models were fitted to determine whether asthma associated with the risk of ruptured AAA in Danish National Registry of Patients and an independent risk of having an AAA at screening in the Viborg vascular trial. From the Danish National Registry of Patients study, asthma diagnosed Conclusions— Recent active asthma increased risk of AAA and ruptured AAA. These findings document and furnish novel links between airway disease and AAA, 2 common diseases that share inflammatory aspects.
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- 2015
163. Deficiency of FcϵR1 Increases Body Weight Gain but Improves Glucose Tolerance in Diet-Induced Obese Mice
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Peter Libby, Cong-Lin Liu, Alexander S. Banks, Yun-Jung Lee, Jun Shirakawa, Meriem Abdennour, Karine Clément, Karine Iamarene, Mengyang Liao, Karen Inouye, Galina K. Sukhova, Rohit N. Kulkarni, Sébastien André, and Guo-Ping Shi
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Male ,medicine.medical_specialty ,Glucose uptake ,Immunoblotting ,Adipose tissue ,Gene Expression ,Mice, Obese ,White adipose tissue ,Diet, High-Fat ,Weight Gain ,Mice ,Endocrinology ,Internal medicine ,Enhancer binding ,3T3-L1 Cells ,medicine ,Adipocytes ,CCAAT-Enhancer-Binding Protein-alpha ,Animals ,Humans ,Obesity ,Original Research ,Mice, Knockout ,Glucose tolerance test ,biology ,medicine.diagnostic_test ,Receptors, IgE ,Reverse Transcriptase Polymerase Chain Reaction ,Glucose Tolerance Test ,Immunoglobulin E ,Obesity, Morbid ,Mice, Inbred C57BL ,PPAR gamma ,Adipose Tissue ,Adipogenesis ,biology.protein ,Female ,RNA Interference ,Energy Metabolism ,Diet-induced obese ,GLUT4 - Abstract
Prior studies demonstrated increased plasma IgE in diabetic patients, but the direct participation of IgE in diabetes or obesity remains unknown. This study found that plasma IgE levels correlated inversely with body weight, body mass index, and body fat mass among a population of randomly selected obese women. IgE receptor FcϵR1-deficient (Fcer1a−/−) mice and diet-induced obesity (DIO) mice demonstrated that FcϵR1 deficiency in DIO mice increased food intake, reduced energy expenditure, and increased body weight gain but improved glucose tolerance and glucose-induced insulin secretion. White adipose tissue from Fcer1a−/− mice showed an increased expression of phospho-AKT, CCAAT/enhancer binding protein-α, peroxisome proliferator-activated receptor-γ, glucose transporter-4 (Glut4), and B-cell lymphoma 2 (Bcl2) but reduced uncoupling protein 1 (UCP1) and phosphorylated c-Jun N-terminal kinase (JNK) expression, tissue macrophage accumulation, and apoptosis, suggesting that IgE reduces adipogenesis and glucose uptake but induces energy expenditure, adipocyte apoptosis, and white adipose tissue inflammation. In 3T3-L1 cells, IgE inhibited the expression of CCAAT/enhancer binding protein-α and peroxisome proliferator-activated receptor-γ, and preadipocyte adipogenesis and induced adipocyte apoptosis. IgE reduced the 3T3-L1 cell expression of Glut4, phospho-AKT, and glucose uptake, which concurred with improved glucose tolerance in Fcer1a−/− mice. This study established two novel pathways of IgE in reducing body weight gain in DIO mice by suppressing adipogenesis and inducing adipocyte apoptosis while worsening glucose tolerance by reducing Glut4 expression, glucose uptake, and insulin secretion.
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- 2015
164. Interaction between allergic asthma and atherosclerosis
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Jin Ying Zhang, Cong-Lin Liu, and Guo-Ping Shi
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0301 basic medicine ,Leukotrienes ,Regulatory T cell ,Population ,030204 cardiovascular system & hematology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,medicine ,Hypersensitivity ,Leukocytes ,Humans ,education ,Asthma ,House dust mite ,education.field_of_study ,biology ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,General Medicine ,biology.organism_classification ,Mast cell ,medicine.disease ,Atherosclerosis ,Lipid Metabolism ,030104 developmental biology ,medicine.anatomical_structure ,Granulocyte macrophage colony-stimulating factor ,Arachidonate 5-lipoxygenase ,Immunology ,biology.protein ,Tumor necrosis factor alpha ,medicine.drug - Abstract
Prior studies have established an essential role of mast cells in allergic asthma and atherosclerosis. Mast cell deficiency or inactivation protects mice from allergen-induced airway hyper-responsiveness and diet-induced atherosclerosis, suggesting that mast cells share pathologic activities in both diseases. Allergic asthma and atherosclerosis are inflammatory diseases that contain similar sets of elevated numbers of inflammatory cells in addition to mast cells in the airway and arterial wall, such as macrophages, monocytes, T cells, eosinophils, and smooth muscle cells. Emerging evidence from experimental models and human studies points to a potential interaction between the two seemingly unrelated diseases. Patients or mice with allergic asthma have a high risk of developing atherosclerosis or vice versa, despite the fact that asthma is a Th2-oriented disease, whereas Th1 immunity promotes atherosclerosis. In addition to the preferred Th1/Th2 responses that may differentiate the two diseases, mast cells and many other inflammatory cells also contribute to their pathogenesis by much more than just T cell immunity. Here we summarize the different roles of airway and arterial wall inflammatory cells and vascular cells in asthma and atherosclerosis, and propose an interaction between the two diseases, although limited investigations are available to delineate the molecular and cellular mechanisms by which one disease increases the risk of the other. Results from mouse allergic asthma and atherosclerosis models and from human population studies lead to the hypothesis that patients with atherosclerosis may benefit from anti-asthmatic medications, or that the therapeutic regimens targeting atherosclerosis may also alleviate allergic asthma.
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- 2015
165. Image-guided percutaneous renal cryoablation for stage 1 renal cell carcinoma with high surgical risk
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Weidong Gan, Rong Yang, Xiaoxiang Chen, Xiang Yan, Wang Wei, Mingxin Zhang, Guo-Ping Shi, Hongqian Guo, Yang Yang, and Yang Wang
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Cryoablation ,Local anesthesia ,Adult ,Male ,medicine.medical_specialty ,Percutaneous ,medicine.medical_treatment ,Cryosurgery ,Surgical oncology ,Renal cell carcinoma ,medicine ,Humans ,Stage (cooking) ,Carcinoma, Renal Cell ,Aged ,Neoplasm Staging ,Aged, 80 and over ,medicine.diagnostic_test ,business.industry ,Research ,Endoscopy ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,Surgery, Computer-Assisted ,Oncology ,Female ,Surgery ,Radiology ,business - Abstract
Background This study was undertaken to evaluate the feasibility, safety, and therapeutic effects of percutaneous renal cryoablation under local anesthesia with conscious sedation for patients who have unresectable stage 1 (T1NoMo) renal cell carcinoma (RCC) in high surgical risk. Methods Eighteen patients who were not candidates for surgery underwent primary cryosurgery guided by gray-scale ultrasound. Contrast-enhanced ultrasonography (CEUS) and contrast-enhanced computed tomography (CT) were performed to evaluate treatment at completion. Results The mean follow-up period was 26.8 months (range, 12–56 months). All tumors were biopsied before cryosurgery. Seventeen tumors remained free of enhancement during follow-up period. No major complications associated with cryoablation procedures were found though two instances of subcapsular hematomas, one of retroperitoneal errhysis and one of nausea, were seen after cryoablation. One patient had a local recurrence of tumor and received additional cryoablation. Local tumor control rate was 100 % of T1NoMo tumors including the recurrence case who underwent additional cryoablation. Conclusions Percutaneous cryoablation can be recommended as a feasible, safe, and promising therapy for the treatment of renal tumor, especially those unresectable stage 1 RCC, with a low risk of complications.
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- 2015
166. Cathepsin K Deficiency Ameliorates Systemic Lupus Erythematosus-like Manifestations in
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Yi, Zhou, Huimei, Chen, Li, Liu, Xueqing, Yu, Galina K, Sukhova, Min, Yang, Vasileios C, Kyttaris, Isaac E, Stillman, Bruce, Gelb, Peter, Libby, George C, Tsokos, and Guo-Ping, Shi
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Mice, Inbred MRL lpr ,Membrane Glycoproteins ,Cathepsin K ,Autoimmunity ,Complement C3 ,Kidney ,Lupus Nephritis ,T-Lymphocytes, Regulatory ,Article ,Mice, Inbred C57BL ,Mice ,Glomerulonephritis ,Toll-Like Receptor 7 ,Immunoglobulin G ,Animals ,Lupus Erythematosus, Systemic ,fas Receptor ,Spleen - Abstract
Cysteinyl cathepsin K (CatK) is expressed in osteoclasts to mediate bone resorption, but it is also inducible under inflammatory conditions. Faslpr mice on a C57BL/6 background develop spontaneous systemic lupus erythematosus (SLE)-like manifestations. While normal mouse kidneys expressed negligible CatK, those from Faslpr mice showed elevated CatK expression in the glomeruli and tubulointerstitial space. Faslpr mice also showed elevated serum CatK levels. CatK deficiency in Faslpr mice reduced all tested kidney pathologies, including glomerulus and tubulointerstitial scores, glomerulus complement C3 and IgG deposition, chemokine expression and macrophage infiltration, and serum autoantibodies. CatK contributed to Faslpr mouse autoimmunity and pathology in part by its activity in Toll-like receptor-7 (TLR7) proteolytic processing and consequent regulatory T cell (Treg) biology. Elevated TLR7 expression and proteolytic processing in Faslpr mouse kidneys and Treg cells showed significantly reduced levels in CatK-deficient mice, leading to increased spleen and kidney Treg content. Purified CD4+CD25highFoxp3+ Treg cells from CatK-deficient mice doubled their immunosuppressive activity against T effector cells, compared to those from CatK-sufficient mice. In Faslpr mice, repopulation of purified Treg cells from CatK-sufficient mice reduced spleen sizes, autoantibody titers, and glomerulus C3 and IgG deposition, and increased splenic and kidney Treg cell contents. Treg cells from CatK-deficient mice had significantly more potency than CatK-sufficient Treg cells in reducing spleen sizes, serum autoantibody titers, and glomerulus C3 deposition, and in increasing splenic and kidney Treg cell content. This study established a possible role of CatK in TLR7 proteolytic activation, Treg immunosuppressive activity, and lupus autoimmunity and pathology.
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- 2015
167. Abstract 418: Microfibrillar-Associated Protein 4 Deficiency Reduces Size and Incidence Rate of Angiotensin II Induced Abdominal Aortic Aneurysms in Mice
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Katrine L Kirketerp-Møller, Jane Stubbe, Anders Schlosser, Karin Kejling, Jesper B Møller, Niels Marcussen, Pernille B Hansen, Guo-Ping Shi, Uffe Holmskov, and Grith L Sørensen
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cardiovascular system ,Cardiology and Cardiovascular Medicine - Abstract
Microfibrillar-associated protein 4 (MFAP4) is an extracellular matrix (ECM) protein primarily located in elastic arteries. It can bind elastin and collagen, and furthermore activate vascular cells through cellular integrin binding and modulate matrix metalloprotinase (MMP) activity. We hypothesized that lack of MFAP4 would decrease vascular inflammation and abdominal aortic aneurysm (AAA) formation. AAA was induced in 9-11 week old mice using two experimental mouse models: 1) Male Mfap4 -/- /ApoE -/- double knock-out (dKO) and ApoE -/- littermate control mice were feed western diet and subjected to continuously angiotensin II (AngII, 1000 ng/kg/min) infusion for 9-28 days via subcutaneous osmotic mini-pumps. Arterial blood pressure was measured in the femoral artery. 2) 1.5 U/mL elastase was infused into the infrarenal aorta in Mfap4 -/- and littermate Mfap4 +/+ mice for 5 minutes. Aortic blood flow was restored and the mice recovered for 9-16 days. Aortic diameter was measured in mice subjected to AngII or elastase infusion at day 28 and 16 respectively. MMP activity was detected by zymography. No difference in AAA formation was observed between genotypes after elastase perfusion. In response to AngII infusion dKO mice showed a significantly decrease in AAA diameter and incidence rate compared to ApoE -/- mice. AngII-induced increase in blood pressure was not dependent of MFAP4. However, there was decreased aortic arch atherosclerotic plaque formation, MMP2 and MMP9 activity in aortic tissue from dKO mice compared to ApoE -/- mice. Furthermore there was a non-significant tendency of decreased elastin degradation score in the AngII infused dKO mice, however this was not observed in the elastase perfused mice. Activity of MMP12 and extent of infiltrating leukocytes in aneurysmal tissue from both models will be further investigated. In conclusion we observed a decreased AAA formation and MMP activity in Mfap4 -/- /ApoE -/- mice which was not explained by variation in blood pressure or altered elastin degradation. The data suggest that MFAP4 induces MMP2-activity and thus the propensity for AAA formation.
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- 2015
168. Plasma cytokine levels and risks of abdominal aortic aneurysms: A population-based prospective cohort study
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Jes S. Lindholt, Jinying Zhang, Cong-Lin Liu, Bingjie Lv, Guo-Ping Shi, Mengyang Liao, Longxian Cheng, Lars Melholt Rasmussen, and Xiang Cheng
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musculoskeletal diseases ,Male ,medicine.medical_specialty ,Denmark ,Blood Pressure ,Enzyme-Linked Immunosorbent Assay ,macromolecular substances ,Gastroenterology ,environment and public health ,Article ,Cohort Studies ,Interferon-gamma ,immune system diseases ,Risk Factors ,Internal medicine ,medicine ,Humans ,cardiovascular diseases ,Prospective Studies ,Risk factor ,Prospective cohort study ,Interleukin 6 ,Aged ,biology ,business.industry ,Interleukin-6 ,C-reactive protein ,Interleukin-17 ,General Medicine ,medicine.disease ,Abdominal aortic aneurysm ,Interleukin-10 ,enzymes and coenzymes (carbohydrates) ,Blood pressure ,C-Reactive Protein ,Cross-Sectional Studies ,Immunology ,biology.protein ,cardiovascular system ,Cytokines ,Female ,IL17A ,business ,Body mass index ,Aortic Aneurysm, Abdominal - Abstract
BACKGROUND: Abdominal aortic aneurysm (AAA) is characterized by inflammatory cell accumulation in AAA lesions that produce inflammatory cytokines and advance its pathogenesis. Peripheral cytokines may predict the degree or risk of AAA.METHODS AND RESULTS: ELISA determined plasma interleukin-6 (IL6), IL10, IL17A, IFN-γ, and C-reactive protein (CRP) from 476 AAA patients and 200 controls. AAA patients had lower IL6, IFN-γ, IL10, IL17A, and higher CRP than controls. IL10 correlated positively with IFN-γ, IL17A, or IL6, but not CRP in control or AAA populations. IL10 associated negatively with systolic blood pressure, whereas CRP associated positively with diastolic blood pressure and body mass index. CRP was an independent AAA risk factor and correlated positively with aortic diameters before and after adjustments for other risk factors. IFN-γ, IL17A, and CRP correlated positively with cross-sectional AAA area after adjustment. IL10 correlated positively with AAA growth rate before and after adjustment. The risk of death doubled in AAA patients with CRP levels above the median.CONCLUSIONS: Reduced IFN-γ, IL10, and IL17A in AAA patients, positive correlations of IFN-γ and IL17A with cross-sectional AAA area, IL10 with AAA growth rate, and IL10 with IFN-γ and IL17A suggest combined Th1, Th2, and Th17 immune responses in human AAAs.
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- 2015
169. Mast Cells as Effectors in Atherosclerosis
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Guo-Ping Shi, Ilze Bot, and Petri T. Kovanen
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Chemokine ,Allergy ,Anti-Inflammatory Agents ,Tryptase ,Inflammation ,Article ,chemistry.chemical_compound ,Immune system ,medicine ,Animals ,Humans ,Mast Cells ,Molecular Targeted Therapy ,biology ,Chymase ,Atherosclerosis ,Mast cell ,medicine.disease ,medicine.anatomical_structure ,chemistry ,Drug Design ,Immunology ,biology.protein ,Inflammation Mediators ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Histamine ,Signal Transduction - Abstract
The mast cell is a potent immune cell known for its functions in host defense responses and diseases, such as asthma and allergies. In the past years, accumulating evidence established the contribution of the mast cell to cardiovascular diseases as well, in particular, by its effects on atherosclerotic plaque progression and destabilization. Through its release not only of mediators, such as the mast cell–specific proteases chymase and tryptase, but also of growth factors, histamine, and chemokines, activated mast cells can have detrimental effects on its immediate surroundings in the vessel wall. This results in matrix degradation, apoptosis, and enhanced recruitment of inflammatory cells, thereby actively contributing to cardiovascular diseases. In this review, we will discuss the current knowledge on mast cell function in cardiovascular diseases and speculate on potential novel therapeutic strategies to prevent acute cardiovascular syndromes via targeting of mast cells.
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- 2015
170. Activated regulatory T-cells attenuate myocardial ischaemia/reperfusion injury through a CD39-dependent mechanism
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Xiao Bo Mao, Tingting Tang, Zheng Feng Zhu, Jiao Jiao, Ke Jing Wang, Yuhua Liao, Shao Fang Nie, Qing Kenneth Wang, Guo-Ping Shi, Bing Jie Lv, Yu Zhi Lu, Xin Tu, Hong Xiao, Xiang Cheng, and Ni Xia
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Male ,Green Fluorescent Proteins ,Ischemia ,Myocardial Reperfusion Injury ,Pharmacology ,Lymphocyte Activation ,T-Lymphocytes, Regulatory ,Chemokine receptor ,Mice ,Troponin complex ,Antigens, CD ,medicine ,Animals ,cardiovascular diseases ,Myocardial infarction ,Gene Knock-In Techniques ,Macrophage inflammatory protein ,Analysis of Variance ,biology ,business.industry ,Apyrase ,FOXP3 ,Forkhead Transcription Factors ,General Medicine ,medicine.disease ,Adoptive Transfer ,Mice, Inbred C57BL ,Myeloperoxidase ,Immunology ,biology.protein ,Immunotherapy ,business ,Reperfusion injury - Abstract
Regulatory T-cells (Tregs) are generally regarded as key immunomodulators that maintain immune tolerance and counteract tissue damage in a variety of immune-mediated disorders. However, its role in myocardial ischaemia/reperfusion injury (MIRI) remains unknown. The purpose of the present study was to determine whether Tregs exert a beneficial effect on mouse MIRI. We examined the role of Tregs in murine MIRI by depletion using 'depletion of regulatory T-cell' (DEREG) mice and adoptive transfer using Forkhead box P3 (Foxp3)-GFP knockin mice and the mechanisms of cardio protection were further studied in vivo and in vitro. Tregs rapidly accumulated in murine hearts following MIRI. Selective depletion of Tregs in the DEREG mouse model resulted in aggravated MIRI. In contrast, the adoptive transfer of in vitro-activated Tregs suppressed MIRI, whereas freshly isolated Tregs had no effect. Mechanistically, activated Treg-mediated protection against MIRI was not abrogated by interleukin (IL)-10 or transforming growth factor (TGF)-β1 inhibition but was impaired by the genetic deletion of cluster of differentiation 39 (CD39). Moreover, adoptive transfer of in vitro-activated Tregs attenuated cardiomyocyte apoptosis, activated a pro-survival pathway involving Akt and extracellular-signal-regulated kinase (ERK) and inhibited neutrophil infiltration, which was compromised by CD39 deficiency. Finally, the peripheral blood mononuclear cells of acute myocardial infarction (AMI) patients after primary percutaneous coronary intervention (PCI) revealed a decrease in CD4+CD25+CD127low Tregs and a relative increase in CD39+ cells within the Treg population. In conclusion, our data validated a protective role for Tregs in MIRI. Moreover, in vitro-activated Tregs ameliorated MIRI via a CD39-dependent mechanism, representing a putative therapeutic strategy.
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- 2015
171. Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter
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Norbert Gerdes, Huimei Chen, Peter Libby, Aina He, Michael A. Shi, Mengmeng Jiang, Jing Wang, Toyoaki Murohara, Cong-Lin Liu, Yi Zhou, Xian Wu Cheng, Petr Jarolim, Gary E. Shull, Jian Liu, Jie Zhang, Chongxiu Sun, Galina K. Sukhova, Masafumi Kuzuya, Shaunessy Rogers, Qiang Ke, René J. M. Bindels, Mengyang Liao, Guo-Ping Shi, David H. Ellison, Sabina K. Jelen, Xiang Cheng, and Chao Ling Yang
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Apolipoprotein E ,Cell signaling ,medicine.medical_specialty ,Chemokine ,medicine.medical_treatment ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Article ,Mice ,Apolipoproteins E ,Internal medicine ,Chlorocebus aethiops ,parasitic diseases ,medicine ,Animals ,Solute Carrier Family 12, Member 3 ,Receptor ,Aorta ,Receptors, Interleukin-18 ,urogenital system ,Macrophages ,Interleukin-18 ,General Medicine ,Atherosclerosis ,Molecular biology ,Interleukin-18 receptor ,Cytokine ,Endocrinology ,Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] ,COS Cells ,embryonic structures ,biology.protein ,Interleukin 18 ,Signal transduction ,Chemokines ,Tunica Intima ,Protein Binding ,Signal Transduction - Abstract
Item does not contain fulltext Interleukin-18 (IL18) participates in atherogenesis through several putative mechanisms. Interruption of IL18 action reduces atherosclerosis in mice. Here, we show that absence of the IL18 receptor (IL18r) does not affect atherosclerosis in apolipoprotein E-deficient (Apoe(-/-)) mice, nor does it affect IL18 cell surface binding to or signaling in endothelial cells. As identified initially by co-immunoprecipitation with IL18, we found that IL18 interacts with the Na-Cl co-transporter (NCC; also known as SLC12A3), a 12-transmembrane-domain ion transporter protein preferentially expressed in the kidney. NCC is expressed in atherosclerotic lesions, where it colocalizes with IL18r. In Apoe(-/-) mice, combined deficiency of IL18r and NCC, but not single deficiency of either protein, protects mice from atherosclerosis. Peritoneal macrophages from Apoe(-/-) mice or from Apoe(-/-) mice lacking IL18r or NCC show IL18 binding and induction of cell signaling and cytokine and chemokine expression, but macrophages from Apoe(-/-) mice with combined deficiency of IL18r and NCC have a blunted response. An interaction between NCC and IL18r on macrophages was detected by co-immunoprecipitation. IL18 binds to the cell surface of NCC-transfected COS-7 cells, which do not express IL18r, and induces cell signaling and cytokine expression. This study identifies NCC as an IL18-binding protein that collaborates with IL18r in cell signaling, inflammatory molecule expression, and experimental atherogenesis. 01 juli 2015
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- 2015
172. Cathepsin L function in insect moulting: molecular cloning and functional analysis in cotton bollworm, Helicoverpa armigera
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Jinsong Liu, Guo-Ping Shi, Wenjuan Zhang, Wenxiong Xu, and Gu-Ren Zhang
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Ecdysone ,DNA, Complementary ,Cathepsin L ,Molecular Sequence Data ,E-64 ,Molting ,Helicoverpa armigera ,chemistry.chemical_compound ,Hemolymph ,Botany ,Genetics ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Enzyme Inhibitors ,Molecular Biology ,Cathepsin ,Gossypium ,Life Cycle Stages ,Base Sequence ,biology ,Gene Expression Profiling ,fungi ,Gene Expression Regulation, Developmental ,biology.organism_classification ,Cathepsins ,Cysteine protease ,Lepidoptera ,Cysteine Endopeptidases ,chemistry ,Biochemistry ,Larva ,Insect Science ,biology.protein ,Instar ,Epidermis ,Protein Processing, Post-Translational ,Sequence Alignment ,Moulting - Abstract
Moulting is an essential process of insect development but little is known about cysteine proteases in the process. Here, we detail a proteolytic activity profile from fifth larval instar to new pupae of the lepidopteran Helicoverpa armigera. At fifth to sixth instar moulting, the activities were significantly higher than those in non-moulting stages, and were inhibited by the cysteine protease inhibitor, 2S, 3S-trans-epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester (E-64), or by the cathepsin L-selective inhibitor CLIK148. Further, a 1513 bp cathepsin L cDNA (Har-CL) was isolated from the H. armigera larval cuticle and epidermis layer. Har-CL gene expression, which is correlated closely with ecdysone, was higher during larval moulting. Injection of E-64 or CLIK148 resulted in delayed fifth to sixth instar moulting, suggesting an essential role for cathepsin L in larval moulting.
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- 2006
173. Elastolytic Cathepsin Induction/Activation System Exists in Myocardium and Is Upregulated in Hypertensive Heart Failure
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Takahiro Kimata, Xian Wu Cheng, Guo-Ping Shi, Eri Asai, Kohzo Nagata, Masako Saka, Hideo Izawa, Mitsuhiro Yokota, Akiko Noda, Toyoaki Murohara, Hai Jin, Tetsuro Nagasaka, Kae Nakamura, Masafumi Kuzuya, Akihisa Iguchi, and Koji Obata
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Adult ,Male ,medicine.medical_specialty ,Vascular smooth muscle ,Cardiomegaly ,Biology ,Muscle hypertrophy ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Humans ,Myocyte ,Aged ,Cathepsin S ,Heart Failure ,Cathepsin ,Rats, Inbred Dahl ,Hydrolysis ,Myocardium ,Anatomy ,Middle Aged ,medicine.disease ,Cathepsins ,Elastin ,Rats ,Up-Regulation ,Enzyme Activation ,Endocrinology ,Heart failure ,Hypertension ,biology.protein ,Immunostaining - Abstract
Cathepsins are cysteine proteases that participate in various types of tissue remodeling. However, their expressions during myocardial remodeling have not been examined. In this study, we investigated their expressions in the left ventricular (LV) myocardium of rats and humans with hypertension-induced LV hypertrophy or heart failure (HF). Real-time PCR and immunoblot analysis revealed that the abundance of cathepsin S mRNA or protein in the LV tissues was greater in rats or humans with HF than in those with hypertrophy or in control subjects. Immunostaining showed that cathepsin S was localized predominantly to cardiac myocytes and coronary vascular smooth muscle cells, but also overlapped in part with macrophages. Elastic lamina fragmentations significantly increased in the LV intramyocardial coronary arteries of HF rats. The amount of elastolytic activity in the extract of the LV myocardium was markedly increased for HF rats compared with controls, and this activity was mostly because of cathepsin S. Although the amount of elastin mRNA was increased in the LV myocardium of HF rats, the area of interstitial elastin was not. The expression of interleukin 1β was increased in the LV myocardium of HF rats, and this cytokine was found to increase the expression and activity of cathepsin S in cultured neonatal cardiomyocytes. These results suggest that cathepsin S participates in pathological LV remodeling associated with hypertension-induced HF. This protease is, thus, a potential target for therapeutics aimed at preventing or reversing cardiac remodeling.
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- 2006
174. Do Cathepsins Play a Role in Abdominal Aortic Aneurysm Pathogenesis?
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Galina K. Sukhova and Guo-Ping Shi
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Pathology ,medicine.medical_specialty ,Proteases ,Apoptosis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Proinflammatory cytokine ,Pathogenesis ,Aortic aneurysm ,History and Philosophy of Science ,medicine ,Animals ,Humans ,Protease Inhibitors ,cardiovascular diseases ,Cathepsin ,Neovascularization, Pathologic ,General Neuroscience ,Atherosclerosis ,medicine.disease ,Cathepsins ,Cysteine protease ,Protease inhibitor (biology) ,Cysteine Endopeptidases ,Cystatin C ,cardiovascular system ,Cancer research ,biology.protein ,Collagen ,Aortic Aneurysm, Abdominal ,medicine.drug - Abstract
Between 1998 and 1999 we suggested a role for cysteine proteases, particularly cathepsins S and K, in atherosclerosis and abdominal aortic aneurysm (AAA) formation. We also demonstrated the presence and activity of cathepsins S, K, and L in atherosclerotic and aneurysmal lesions in humans. Features unique to this family of extracellular enzymes indicate its likely participation in these vascular diseases. As very potent elastolytic enzymes, cathepsins are strong candidates as key participants in aneurysm development. Importantly, cathepsins express very high elastolytic activity in AAA due to reciprocal correlation with cystatin C, their most abundant endogenous inhibitor. Two opposite processes coexist in aneurysmal tissue: overexpression of elastolytic cathepsins, and severe suppression of cystatin C, probably due to differentially regulated expression and secretion of cathepsins and their inhibitors in response to inflammatory cytokines. Involvement of cathepsins in microvessel formation, a pathophysiological marker of human AAA, and programmed cell death (apoptosis), increases the likelihood of cathepsin participation in AAA formation and growth. We also summarize here results obtained in our and other laboratories that demonstrated reduced atherosclerosis and AAA in in vivo models using mice lacking different cathepsins. Deficiency of cysteine protease inhibitor cystatin C in atherosclerosis-prone ApoE-null mice leads to the development of specific features of AAA such as thinning of the tunica media and aortic dilatation. Taken together, such findings in humans in vitro with different cell types and in vivo in genetically altered mice demonstrate the importance of cysteine protease/protease inhibitor balance in dysregulated arterial integrity and remodeling during atherosclerosis and aortic aneurysm formation.
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- 2006
175. Atherosclerosis progression and monocyte emigration from plaque
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René R. Sevag Packard and Guo-Ping Shi
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Apolipoprotein E ,Pathology ,medicine.medical_specialty ,business.industry ,Monocyte ,Dendritic cell ,Transplantation ,chemistry.chemical_compound ,medicine.anatomical_structure ,Immune system ,chemistry ,Lysophosphatidic acid ,medicine ,Molecular Medicine ,Cardiology and Cardiovascular Medicine ,business ,Lymph node ,Lipoprotein - Abstract
Evaluation of: Llodrá J, Angeli V, Liu J, Trogan E, Fisher AE, Randolph GJ: Emigration of monocyte-derived cells from atherosclerotic lesions characterizes regressive, but not progressive, plaques. Proc. Natl Acad. Sci. 101(32), 11779–11784 (2004). Monocyte/macrophages are the most abundant immune cells in atherosclerotic lesions, in which they have a pivotal role. The present study sought to determine how dyslipidemia affects the fate of monocytes recruited to the plaque. The researchers used an in vitro model of a vessel wall to demonstrate that platelet-activating factor and lysophosphatidic acid, two key mediators of atherosclerosis, impair the ability of monocytes to emigrate from the cultured vessel wall. Furthermore, an in vivo model of aortic arch transplantation demonstrated reduced emigration of monocyte-derived cells into the plaque-draining lymph node under conditions of atherosclerotic lesion progression. Thus, progression of atherosclerosis is characterized by reduced emigration of monocyte-derived cells in addition to new monocyte recruitment into the vessel wall. Interestingly, these sequestered cells bear features reminiscent of dendritic cells, which may locally exacerbate inflammatory reactions.
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- 2006
176. Increased serum cathepsin S in patients with atherosclerosis and diabetes
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Guo-Ping Shi, Huanxian Fu, Jiusong Sun, An Ren, Chengcheng Hu, Zimin Sun, Jian Liu, Wei-Hua Xu, Gengxing Yan, Likun Ma, and Jintian Yang
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Male ,medicine.medical_specialty ,Population ,Renal function ,Cathepsin C ,chemistry.chemical_compound ,Diabetes mellitus ,Internal medicine ,Diabetes Mellitus ,medicine ,Humans ,Cystatin C ,education ,Aged ,Cathepsin S ,Creatinine ,education.field_of_study ,biology ,business.industry ,Middle Aged ,Atherosclerosis ,medicine.disease ,Cathepsins ,Cystatins ,Endocrinology ,chemistry ,biology.protein ,Female ,Cystatin ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers - Abstract
Atherosclerosis and diabetes are closely associated and both involve extensive degradation of the aortic elastin. Increased elastase activity has been detected in diabetic animal aortae. We have demonstrated enhanced elastolytic cathepsin S in human atherosclerotic lesions but insufficient amounts of its endogenous inhibitor cystatin C, suggesting alterations of serum cathepsin S and/or cystatin C in patients with atherosclerosis or diabetes. In this study, we measured levels of both cathepsin S and cystatin C in sera from 240 patients by ELISA. Among these patients, 107 had a diagnosis of atherosclerotic stenosis, 103 were diabetic, and 30 had neither condition. Multiple linear regression analysis demonstrated that significantly higher serum levels of cathepsin S in patients with either atherosclerotic stenosis (p
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- 2006
177. Cathepsin S Controls Angiogenesis and Tumor Growth via Matrix-derived Angiogenic Factors
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Anders Grubb, Raghu Kalluri, Shiro Kitamoto, Bing Wang, Harold A. Chapman, Jiusong Sun, Min Yang, and Guo-Ping Shi
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Collagen Type IV ,Angiogenesis ,Recombinant Fusion Proteins ,medicine.medical_treatment ,Basic fibroblast growth factor ,Cathepsin D ,Angiogenesis Inhibitors ,Mice, Transgenic ,Biology ,Autoantigens ,Biochemistry ,Mice ,chemistry.chemical_compound ,Neoplasms ,Cathepsin L1 ,medicine ,Animals ,Humans ,Protease Inhibitors ,Cystatin C ,Molecular Biology ,Cell Proliferation ,Cathepsin S ,Mice, Knockout ,Cathepsin ,Neovascularization, Pathologic ,Growth factor ,Cell Biology ,Cathepsins ,Cystatins ,Peptide Fragments ,Endostatins ,Extracellular Matrix ,Mice, Inbred C57BL ,Pancreatic Neoplasms ,Survival Rate ,chemistry ,Cancer research ,Angiogenesis Inducing Agents ,Cystatin ,Cell Adhesion Molecules - Abstract
The cysteine protease cathepsin S is highly expressed in malignant tissues. By using a mouse model of multistage murine pancreatic islet cell carcinogenesis in which cysteine cathepsin activity has been functionally implicated, we demonstrated that selective cathepsin S deficiency impaired angiogenesis and tumor cell proliferation, thereby impairing angiogenic islet formation and the growth of solid tumors, whereas the absence of its endogenous inhibitor cystatin C resulted in opposite phenotypes. Although mitogenic vascular endothelial growth factor, transforming growth factor-beta1, and the anti-angiogenic endostatin levels in either serum or carcinoma tissue extracts did not change in cathepsin S- or cystatin C-null mice, tumor tissue basic fibroblast growth factor and serum type 1 insulin growth factor levels were higher in cystatin C-null mice, and serum type 1 insulin growth factor levels were also increased in cathepsin S-null mice. Furthermore, cathepsin S affected the production of type IV collagen-derived anti-angiogenic peptides and the generation of bioactive pro-angiogenic gamma2 fragments from laminin-5, revealing a functional role for cathepsin S in angiogenesis and neoplastic progression.
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- 2006
178. Cathepsin L expression and regulation in human abdominal aortic aneurysm, atherosclerosis, and vascular cells
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Jin Tian Yang, Wei Hua Xu, Likun Ma, Peter Libby, Gregory Dolganov, Galina K. Sukhova, Chengcheng Hu, Jian Liu, Guo-Ping Shi, Jiusong Sun, An Ren, and Huanxiang Fu
- Subjects
Pathology ,medicine.medical_specialty ,Cell type ,Cathepsin L ,medicine.medical_treatment ,Enzyme-Linked Immunosorbent Assay ,Inflammation ,In Vitro Techniques ,Polymerase Chain Reaction ,Muscle, Smooth, Vascular ,Mice ,Aortic aneurysm ,medicine ,Animals ,Humans ,Saphenous Vein ,RNA, Messenger ,Cells, Cultured ,Enzyme Precursors ,biology ,business.industry ,Atherosclerosis ,medicine.disease ,Cathepsins ,Endothelial stem cell ,Cysteine Endopeptidases ,Cytokine ,Gene Expression Regulation ,Macrophages, Peritoneal ,biology.protein ,Collagenase ,Endothelium, Vascular ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Elastin ,Aortic Aneurysm, Abdominal ,medicine.drug - Abstract
The cysteine protease cathepsin L is one of the most potent mammalian elastases and collagenases, widely expressed at basal levels in most tested tissues and cell types, and regulated by pro-inflammatory stimuli. The inflammatory arterial diseases abdominal aortic aneurysm (AAA) and atherosclerosis involve extensive vascular remodeling that requires elastolysis and collagenolysis. This study examined the hypothesis that cathepsin L is over-expressed in human AAA and atherosclerotic lesions and its expression in vascular cell types found in these lesions is regulated by pro-inflammatory cytokines. Immunohistochemical and tissue extract immunoblot analysis demonstrated increased expression of cathepsin L in human AAA and atheromata and localized its expression to lesional smooth muscle cells (SMC), endothelial cells (EC), and macrophages. In primary cultured human SMC, EC, and monocyte-derived macrophages, pro-inflammatory cytokines or growth factors induced the expression of cathepsin L and its activity against extracellular collagen and elastin. Patients with coronary artery stenosis (n=65) had higher serum cathepsin L levels than those without lesions detectable by quantitative coronary angiography (n=30) (1.47+/-0.33 ng/ml versus 0.60+/-0.06 ng/ml, p0.02). A strong correlation between the percent of stenosis of left anterior descending coronary artery and serum cathepsin L levels in patients with stenosis (R=0.542, p0.0001), also suggests involvement of cathepsin L in these vascular diseases.
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- 2006
179. Abstract 11017: Renin Inhibition Reduces Atherosclerotic Plaque Neovessel Formation and Regresses Advanced Atherosclerotic Plaques
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Mutsuharu Hayashi, Hongxian Wu, Guo-Ping Shi, Xian Wu Cheng, Masafumi Kuzuya, Chang-Ning Hao, Lina Hu, Toyoaki Murohara, Kyosuke Takeshita, and Mohammad Shoaib Hamrah
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Apolipoprotein E ,medicine.medical_specialty ,CATS ,Apolipoprotein B ,biology ,medicine.drug_class ,business.industry ,Hydralazine ,Aliskiren ,Renin inhibitor ,Angiotensin II ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Physiology (medical) ,Internal medicine ,Renin–angiotensin system ,medicine ,biology.protein ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Background: The interaction between the renin-angiotensin system and toll-like receptors (TLRs) in the pathogenesis of advanced atherosclerotic plaques is not well understood. We studied the effects of the renin inhibitor aliskiren on the progression of advanced atherosclerotic plaque in apolipoprotein E-deficient (ApoE –/– ) mice with a special focus on plaque neovessel formation. Methods and Results: Four-wk-old ApoE –/– mice were fed a high-fat diet for 8 wks, and the mice were randomly assigned to one of three groups and administered a vehicle, hydralazine, or aliskiren for an additional 12 wks. Aliskiren reduced the atherosclerotic plaque area and plaque neovessel density. It increased the plaque collagen and elastin contents, and reduced plasma angiotensin II levels and plaque macrophage infiltration and cathepsin S (CatS) protein. Aliskiren also decreased the levels of AT1R, gp91phox, TLR2, monocyte chemotactic protein-1, and CatS mRNAs in the aortic roots. Hydralazine had no beneficial vascular effects, although its administration resulted in the same degree of blood pressure reduction as aliskiren. CatS deficiency mimicked the aliskiren-mediated vasculoprotective effect in the ApoE –/– mice, but aliskiren showed no further benefits in ApoE –/– CatS –/– mice. In vitro , CatS mRNA expressions were induced by angiotensin II, and TLR2 silencing reduced CatS mRNA expression. Moreover, the genetic inhibition of CatS impaired the endothelial cell angiogenic action in vitro and ex vivo . Conclusion: Renin inhibition appears to inhibit advanced plaque neovessel formation in ApoE –/– mice and to decrease the vascular inflammatory action and extracellular matrix degradation, partly by reducing AT1R/TLR2-mediated CatS activation and activity, thus regressing advanced atherosclerotic plaques.
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- 2014
180. Lysosomal Cysteine Proteases in Atherosclerosis
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Peter Libby, Wei-Hua Xu, Guo-Ping Shi, Galina K. Sukhova, Jian Liu, and Jiusong Sun
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Proteases ,Arteriosclerosis ,Cysteine Proteinase Inhibitors ,Biology ,Matrix metalloproteinase ,Muscle, Smooth, Vascular ,Extracellular matrix ,Mice ,Apolipoproteins E ,Thromboembolism ,Cell Adhesion ,Leukocytes ,medicine ,Animals ,Humans ,Cystatin C ,Cathepsin S ,Mice, Knockout ,Cathepsin ,Extracellular Matrix Proteins ,Macrophages ,Cystatins ,Protease inhibitor (biology) ,Chemotaxis, Leukocyte ,Cysteine Endopeptidases ,Receptors, LDL ,Biochemistry ,Enzyme Induction ,Disease Progression ,biology.protein ,Cytokines ,Endothelium, Vascular ,Cystatin ,Lysosomes ,Cardiology and Cardiovascular Medicine ,medicine.drug - Abstract
Atherosclerosis is an inflammatory disease characterized by extensive remodeling of the extracellular matrix architecture of the arterial wall. Although matrix metalloproteinases and serine proteases participate in these pathologic events, recent data from atherosclerotic patients and animals suggest the participation of lysosomal cysteine proteases in atherogenesis. Atherosclerotic lesions in humans overexpress the elastolytic and collagenolytic cathepsins S, K, and L but show relatively reduced expression of cystatin C, their endogenous inhibitor, suggesting a shift in the balance between cysteine proteases and their inhibitor that favors remodeling of the vascular wall. Extracts of human atheromatous tissue show greater elastolytic activity in vitro than do those from healthy donors. The cysteinyl protease inhibitor E64d limits this increased elastolysis, indicating involvement of cysteine proteases in elastin degradation during atherogenesis. Furthermore, inflammatory cytokines augment expression and secretion of active cysteine proteases from cultured monocyte-derived macrophages, vascular smooth muscle cells, and endothelial cells and increase degradation of extracellular elastin and collagen. Cathepsin S–deficient cells or those treated with E64d show significantly impaired elastolytic or collagenolytic activity. Additionally, recent in vivo studies of atherosclerosis-prone, LDL receptor–null mice lacking cathepsin S show participation of this enzyme in the initial infiltration of leukocytes, medial elastic lamina degradation, endothelial cell invasion, and neovascularization, illustrating an important role for cysteine proteases in arterial remodeling and atherogenesis.
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- 2004
181. Macrophage Migration Inhibitory Factor Deficiency Impairs Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient Mice
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Jing Tian Yang, John R. David, Kenneth C. Fang, Peter Libby, Christine N. Metz, Galina K. Sukhova, R. Bucala, Guo-Ping Shi, Huanxiang Fu, Tao Xie, Harold A. Chapman, Abhay R. Satoskar, Bing Wang, Daniel I. Simon, Yaou Zhang, and Jie Hong Pan
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medicine.medical_specialty ,Arteriosclerosis ,Muscle, Smooth, Vascular ,Proinflammatory cytokine ,Lesion ,Mice ,Physiology (medical) ,medicine.artery ,Internal medicine ,Animals ,Medicine ,Genetic Predisposition to Disease ,Aorta, Abdominal ,Collagenases ,Receptor ,Macrophage Migration-Inhibitory Factors ,Crosses, Genetic ,Mice, Knockout ,Aorta ,Pancreatic Elastase ,business.industry ,medicine.disease ,Lipids ,Intramolecular Oxidoreductases ,Mice, Inbred C57BL ,Cysteine Endopeptidases ,Endocrinology ,Receptors, LDL ,Enzyme Induction ,Immunology ,LDL receptor ,Diet, Atherogenic ,Macrophage migration inhibitory factor ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Cell Division ,Lipoprotein ,Calcification - Abstract
Background— Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine expressed widely by vascular cells. However, scant in vivo evidence supports direct participation of MIF in atherogenesis. Therefore, we investigated whether deficiency of MIF modulates atherosclerotic lesion formation and composition in low-density lipoprotein receptor-deficient (LDLr −/− ) mice. Methods and Results— MIF −/− LDLr −/− and LDLr −/− mice were generated and consumed an atherogenic diet for 12 or 26 weeks. MIF −/− LDLr −/− mice had significantly reduced abdominal aorta lipid deposition and intimal thickening from aortic arch throughout the abdominal aorta compared with LDLr −/− mice. Marked retardation of atherosclerosis over time in MIF-deficient mice accompanied decreased lesion cell proliferation. At 26 weeks, 20% of MIF-deficient mice developed only early, fatty streak-like lesions, whereas >80% of LDLr −/− mice developed advanced lesions containing calcification and lipid cores. Analysis of smooth muscle cells from mouse aortae demonstrated that MIF deficiency reduced smooth muscle cell proliferation, cysteine protease expression, and elastinolytic and collagenolytic activities. Conclusions— Deficiency of MIF reduces atherogenesis in LDLr −/− mice. These results provide novel insight into inflammatory pathways operating in atheromata and identify a new potential target for modulating atherogenesis.
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- 2004
182. Role of elastolytic cathepsins in vascular remodeling
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Peter Libby, Galina K. Sukhova, and Guo-Ping Shi
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Cathepsin ,Pathology ,medicine.medical_specialty ,biology ,Chemistry ,General Medicine ,medicine.disease ,Internal elastic lamina ,Pathogenesis ,Extracellular matrix ,Atheroma ,Cystatin C ,cardiovascular system ,biology.protein ,medicine ,Elastin ,Cathepsin S - Abstract
Background: We now appreciate that the pathogenesis of atherosclerosis and abdominal aortic aneurysms (AAA) involves breakdown of extracellular matrix (ECM). We recently implicated certain elastolytic cysteine-dependent cathepsins in these pathological processes. Results: We demonstrated over-expression of cathepsins S and K in human atheroma and AAA, and colocalized cathepsin S-positive medial smooth muscle cells (SMC) with sites of internal elastic lamina fragmentation. Moreover, depletion of cystatin C, the most abundant endogenous cysteine protease inhibitor, characterizes both atherosclerotic and aneurysmal lesions. To test directly the role of cathepsin S in atherogenesis, we studied targeted disruption of the cathepsin S gene in atherosclerosis-prone LDLr−/− mice consuming a western diet. Cathepsin S deficiency significantly decreased atherosclerosis. Better-preserved elastin in the tunica media of cathepsin S-deficient mice likely reflected the diminished elastolytic activity in cathepsin S−/− SMC (p
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- 2004
183. Cathepsin S deficiency improves muscle mass loss and dysfunction via the modulation of protein metabolism in mice under pathological stress conditions.
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Ying Wan, Limei Piao, Shengnan Xu, Aiko Inoue, Xiangkun Meng, Yanna Lei, Zhe Huang, Hailong Wang, Xueling Yue, Guo-Ping Shi, Masafumi Kuzuya, and Xian Wu Cheng
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- 2023
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184. Deficiency of cathepsin S reduces atherosclerosis in LDL receptor–deficient mice
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Galina K. Sukhova, Yaou Zhang, Jie-Hong Pan, Youichiro Wada, Takashi Yamamoto, Makoto Naito, Tatsuhiko Kodama, Sotirios Tsimikas, Joseph L. Witztum, Michael L. Lu, Yasuhiko Sakara, Michael T. Chin, Peter Libby, and Guo-Ping Shi
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Arteriosclerosis ,Macrophages ,General Medicine ,Cathepsins ,Article ,Muscle, Smooth, Vascular ,Elastin ,Mice, Inbred C57BL ,Mice ,Receptors, LDL ,Cell Movement ,Leukocytes ,Animals ,lipids (amino acids, peptides, and proteins) ,Collagen ,Rabbits - Abstract
Human atherosclerotic lesions overexpress the lysosomal cysteine protease cathepsin S (Cat S), one of the most potent mammalian elastases known. In contrast, atheromata have low levels of the endogenous Cat S inhibitor cystatin C compared with normal arteries, suggesting involvement of this protease in atherogenesis. The present study tested this hypothesis directly by crossing Cat S-deficient (CatS(-/-)) mice with LDL receptor-deficient (LDLR(-/-)) mice that develop atherosclerosis on a high-cholesterol diet. Compared with LDLR(-/-) mice, double-knockout mice (CatS(-/-)LDLR(-/-)) developed significantly less atherosclerosis, as indicated by plaque size (plaque area and intimal thickening) and stage of development. These mice also had markedly reduced content of intimal macrophages, lipids, smooth muscle cells, collagen, CD4(+) T lymphocytes, and levels of IFN-gamma. CatS(-/-)LDLR(-/-) monocytes showed impaired subendothelial basement membrane transmigration, and aortas from CatS(-/-)LDLR(-/-) mice had preserved elastic laminae. These findings establish a pivotal role for Cat S in atherogenesis.
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- 2003
185. Macrophage migration inhibitory factor is associated with aneurysmal expansion
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John A. Baugh, Peter Libby, Christine N. Metz, Jes S. Lindholt, Guo-Ping Shi, Seamas C. Donnelly, Eskild W. Henneberg, Richard Bucala, Galina K. Sukhova, and Jie-Hong Pan
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Carotid Artery Diseases ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Enzyme-Linked Immunosorbent Assay ,030204 cardiovascular system & hematology ,Muscle, Smooth, Vascular ,Proinflammatory cytokine ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,medicine.artery ,Internal medicine ,medicine ,Humans ,Macrophage ,10. No inequality ,Macrophage Migration-Inhibitory Factors ,Aorta ,Aged ,030304 developmental biology ,0303 health sciences ,Vascular disease ,business.industry ,Macrophages ,medicine.disease ,Immunohistochemistry ,3. Good health ,Surgery ,Carotid Arteries ,Cytokine ,Endocrinology ,Case-Control Studies ,cardiovascular system ,Disease Progression ,Macrophage migration inhibitory factor ,Endothelium, Vascular ,Cardiology and Cardiovascular Medicine ,business ,Aortic Aneurysm, Abdominal - Abstract
Background: Macrophage migration inhibitory factor (MIF) is an inflammatory cytokine released mainly from macrophages and activated lymphocytes. Both atherosclerosis and abdominal aortic aneurysm (AAA) are inflammatory diseases tightly linked to the function of these cells. The correlation and contribution of MIF to these human diseases remain unknown, although a recent rabbit study showed expression of this cytokine in atherosclerotic lesions. Material and Methods: MIF immunohistochemistry was performed on tissue sections from five normal aortas, seven atherosclerotic carotids, and six AAAs. A group of 112 men with small AAAs (defined as 3 to 5 cm) was recruited at the time of diagnosis, had serum samples taken, and was followed annually for 1 to 5 years (mean, 2.9 years) and referred for surgery if the AAA exceeded 5 cm in diameter. Of this study group, 98 had serum MIF measured with an enzyme-linked immunosorbent assay and 61 had detectable levels. Results: In human atherosclerotic and aneurysmal lesions, MIF protein colocalized in macrophages, endothelial cells, and smooth muscle cells, but normal arteries had negligible MIF expression. Furthermore, serum-MIF levels correlated significantly with annual expansion rate ( r = 0.28; P =.005), persisting after adjustment for initial AAA size, smoking habits, diastolic blood pressure, ankle blood pressure index, and age. After exclusion of 38 cases with MIF levels below the detection limit, initial AAA size was also significantly correlated with the MIF levels ( r = 0.42; P =.001), persisting after adjustment for similar confounders, and the correlation coefficient with expansion rate increased to 0.42 ( P =.001). Conclusion: Highly expressed MIF in macrophages, endothelial cells, and smooth muscle cells in lesions from atherosclerosis and AAA and significant association between serum MIF level and AAA initial size and AAA expansion rate in a group of patients with AAA suggest a potential involvement of this proinflammatory cytokine in the pathogenesis of these cardiovascular diseases. (J Vasc Surg 2003;37:628-35.)
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- 2003
186. CD31: beyond a marker for endothelial cells
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Guo-Ping Shi and Li Liu
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Male ,CD31 ,Physiology ,Cell adhesion molecule ,Angiogenesis ,Angiotensin II ,Anti-Inflammatory Agents ,Aortic Diseases ,Immunoglobulin domain ,Biology ,Atherosclerosis ,Molecular biology ,Article ,Platelet Endothelial Cell Adhesion Molecule-1 ,Biochemistry ,Physiology (medical) ,cardiovascular system ,Animals ,Platelet activation ,Tyrosine ,Peptides ,Cardiology and Cardiovascular Medicine ,Aortic Aneurysm, Abdominal ,Proto-oncogene tyrosine-protein kinase Src - Abstract
This editorial refers to ‘A CD31-derived peptide prevents angiotensin II-induced atherosclerosis progression and aneurysm formation’ by G. Fornasa et al. , pp. 30–37, this issue. CD31 is a 130 kDa platelet–endothelial cell (EC) adhesion molecule that was initially identified from ECs and platelets1 and later from blood leucocytes.2 Mature CD31 contains a short [22-amino acid (aa)] NH2-terminal peptide followed by six C2-type immunoglobulin (Ig) domains, each flanked by two conserved cysteine residues outside the cells,3 a 19 aa transmembrane domain, and a 118 aa cytoplasmic tail containing two immunotyrosine-based inhibitory motifs (ITIM)4 ( Figure 1 ) that mediate intracellular signalling. Although CD31 was initially classified as a cell adhesion molecule,3 later studies suggested that CD31 triggers downstream inhibitory signalling4 upon transhomophilic CD31 engagement during cell–cell interaction.5 CD31 signalling participates in the regulation of leucocyte detachment, T-cell activation, platelet activation, and angiogenesis, all of which are critical to the pathogenesis of atherosclerosis and abdominal aortic aneurysms (AAAs). Figure 1 CD31 protein domains and their corresponding cellular functions. Ig, immunoglobulin; aa, amino acid; ITIM, immunotyrosine-based inhibitory motif; SHP2, Src homology-2 phosphatase; Y, tyrosine. Fornasa et al. 6 use atherosclerosis-prone apolipoprotein E-deficient ( Apoe−/− ) mice to demonstrate that aa551–574—a synthetic peptide located to the carboxyl-terminal of the Ig domain 6 ( Figure 1 )—suppressed angiotensin II (Ang-II) perfusion-induced AAAs and atherosclerosis. This peptide reduced atherosclerotic lesion and peri-aortic leucocyte infiltration and increased collagen deposition in aortic root atherosclerotic plaques and the abdominal aorta. Although we typically use CD31 as …
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- 2012
187. Cathepsin G deficiency reduces peri-aortic calcium chloride injury-induced abdominal aortic aneurysms in mice
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Jing Wang, Petri T. Kovanen, Adam Lesner, Peter Libby, Galina K. Sukhova, Jian Liu, Keith C. Ozaki, and Guo-Ping Shi
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medicine.medical_specialty ,Pathology ,Cathepsin G ,Angiogenesis ,Myocytes, Smooth Muscle ,Inflammation ,Enzyme-Linked Immunosorbent Assay ,030204 cardiovascular system & hematology ,Matrix metalloproteinase ,Peptidyl-Dipeptidase A ,Article ,Lesion ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Internal medicine ,Medicine ,Animals ,Humans ,Aorta, Abdominal ,030304 developmental biology ,0303 health sciences ,biology ,business.industry ,Angiotensin II ,Macrophages ,Immunohistochemistry ,Endocrinology ,chemistry ,cardiovascular system ,biology.protein ,Surgery ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Elastin ,Aortic Aneurysm, Abdominal - Abstract
Objective Cathepsin G (CatG) is a serine protease that mediates angiotensin I to angiotensin II (Ang-II) conversion and is highly expressed in human abdominal aortic aneurysms (AAAs). However, it remains untested whether this protease participates in the pathogenesis of AAA. Methods and Results Immunofluorescent double staining demonstrated the expression of CatG in smooth muscle cells (SMCs), macrophages, and endothelial cells in human AAA lesions (n = 12) but not in AAA-free aortas (n = 10). Whereas inflammatory cytokines induced CatG expression, high glucose concentration increased CatG activity in producing Ang-II and angiotensin-converting enzyme in SMCs, which could be fully blocked by a CatG-selective inhibitor or its small interfering RNA. To test whether CatG contributes to AAA development, we generated CatG and low-density lipoprotein receptor double deficient ( Ldlr −/− Ctsg −/− ) mice and their littermate controls ( Ldlr −/− Ctsg +/+ ). Absence of CatG did not affect Ang-II infusion-induced AAAs. In contrast, in Ang-II-independent AAAs induced by periaortic CaCl 2 injury (n = 12 per group), CatG deficiency significantly reduced aortic diameter increase (58.33% ± 6.83% vs 31.67% ± 5.75%; P = .007), aortic lesion area (0.35 ± 0.04 mm 2 vs 0.21 ± 0.02 mm 2 ; P = .005), and aortic wall elastin fragmentation grade (2.75 ± 0.18 vs 1.58 ± 0.17; P = .002) along with reduced lesion collagen content grade (2.80 ± 0.17 vs 2.12 ± 0.17; P = .009) without affecting indices of lesion inflammation, angiogenesis, cell proliferation, or apoptosis. In vitro elastin degradation assays demonstrated that CaCl 2 -induced AAA lesions from Ldlr −/− Ctsg −/− mice contained much lower elastinolytic activity than in those from littermate control mice. Gelatin gel zymogram assay suggested that absence of CatG in CaCl 2 -induced AAA lesions also reduced the activity of elastinolytic matrix metalloproteinases 2 and 9. Conclusions CatG may contribute to CaCl 2 -induced experimental AAAs directly through its elastinolytic activity and indirectly by regulating lesion matrix metalloproteinases 2 and 9 activities. Increased expression of CatG in vascular and inflammatory cells of human AAAs and its increased activity in producing Ang-II and angiotensin-converting enzyme by SMCs suggest an additional mechanism by which CatG contributes to AAA lesion progression.
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- 2014
188. IgE actions on CD4+ T cells, mast cells, and macrophages participate in the pathogenesis of experimental abdominal aortic aneurysms
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Michael A. Shi, Yi Wang, Meixiang Xiang, Galina K. Sukhova, Aina He, Jes S. Lindholt, Jing Wang, Mingcan Xia, Jian-an Wang, Peter Libby, Han Chen, Na Xiong, and Guo-Ping Shi
- Subjects
Apolipoprotein E ,CD4-Positive T-Lymphocytes ,Male ,Adoptive cell transfer ,Macrophage ,T cells ,macrophage ,macromolecular substances ,030204 cardiovascular system & hematology ,Biology ,CD8-Positive T-Lymphocytes ,Immunoglobulin E ,Mast cell ,03 medical and health sciences ,Mice ,0302 clinical medicine ,abdominal aortic aneurysm ,medicine ,Animals ,Humans ,Mast Cells ,cardiovascular diseases ,Research Articles ,030304 developmental biology ,0303 health sciences ,Receptors, IgE ,FCER1 ,Macrophages ,FCER1A ,Adoptive Transfer ,3. Good health ,Up-Regulation ,Mice, Inbred C57BL ,Interleukin 10 ,medicine.anatomical_structure ,Immunology ,biology.protein ,cardiovascular system ,Molecular Medicine ,Abdominal aortic aneurysm ,Female ,IgE ,mast cell ,CD8 ,Gene Deletion ,Aortic Aneurysm, Abdominal - Abstract
Immunoglobulin E (IgE) activates mast cells (MCs). It remains unknown whether IgE also activates other inflammatory cells, and contributes to the pathogenesis of abdominal aortic aneurysms (AAAs). This study demonstrates that CD4+ T cells express IgE receptor FcεR1, at much higher levels than do CD8+ T cells. IgE induces CD4+ T-cell production of IL6 and IFN-γ, but reduces their production of IL10. FcεR1 deficiency (Fcer1a-/-) protects apolipoprotein E-deficient (Apoe-/-) mice from angiotensin-II infusion-induced AAAs and reduces plasma IL6 levels. Adoptive transfer of CD4+ T cells (but not CD8+ T cells), MCs, and macrophages from Apoe-/- mice, but not those from Apoe-/- Fcer1a-/- mice, increases AAA size and plasma IL6 in Apoe-/- Fcer1a-/- recipient mice. Biweekly intravenous administration of an anti-IgE monoclonal antibody ablated plasma IgE and reduced AAAs in Apoe-/- mice. Patients with AAAs had significantly higher plasma IgE levels than those without AAAs. This study establishes an important role of IgE in AAA pathogenesis by activating CD4+ T cells, MCs, and macrophages and supports consideration of neutralizing plasma IgE in the therapeutics of human AAAs.
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- 2014
189. Renin inhibition reduces atherosclerotic plaque neovessel formation and regresses advanced atherosclerotic plaques
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Kyosuke Takeshita, Hongxian Wu, Chang Ning Hao, Xian Wu Cheng, Toyoaki Murohara, Lina Hu, Guo-Ping Shi, Masafumi Kuzuya, Mutsuharu Hayashi, and Mohammad Shoaib Hamrah
- Subjects
Apolipoprotein E ,Male ,Apolipoprotein B ,Blood Pressure ,chemistry.chemical_compound ,Mice ,Fumarates ,Renin ,Aorta ,Chemokine CCL2 ,Mice, Knockout ,biology ,Neovascularization, Pathologic ,Angiotensin II ,Intracellular Signaling Peptides and Proteins ,Nuclear Proteins ,Hydralazine ,Immunohistochemistry ,Plaque, Atherosclerotic ,Extracellular Matrix ,Treatment Outcome ,Cardiology and Cardiovascular Medicine ,medicine.drug ,medicine.medical_specialty ,medicine.drug_class ,Vascular Cell Adhesion Molecule-1 ,Renin inhibitor ,Receptor, Angiotensin, Type 1 ,Apolipoproteins E ,Internal medicine ,Renin–angiotensin system ,medicine ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Gene Silencing ,business.industry ,Macrophages ,Aliskiren ,Atherosclerosis ,Amides ,Cathepsins ,Toll-Like Receptor 2 ,Mice, Inbred C57BL ,Endocrinology ,chemistry ,biology.protein ,business ,Carrier Proteins ,Elastin - Abstract
The interaction between the renin-angiotensin system and toll-like receptors (TLRs) in the pathogenesis of advanced atherosclerotic plaques is not well understood. We studied the effects of the renin inhibitor aliskiren on the progression of advanced atherosclerotic plaque in apolipoprotein E-deficient (ApoE(-/-)) mice with a special focus on plaque neovessel formation.Four-wk-old ApoE(-/-) mice were fed a high-fat diet for 8 wks, and the mice were randomly assigned to one of three groups and administered a vehicle, hydralazine, or aliskiren for an additional 12 wks. Aliskiren reduced the atherosclerotic plaque area and plaque neovessel density. It increased the plaque collagen and elastin contents, and reduced plasma angiotensin II levels and plaque macrophage infiltration and cathepsin S (CatS) protein. Aliskiren also decreased the levels of AT1R, gp91phox, TLR2, monocyte chemotactic protein-1, and CatS mRNAs in the aortic roots. Hydralazine had no beneficial vascular effects, although its administration resulted in the same degree of blood pressure reduction as aliskiren. CatS deficiency mimicked the aliskiren-mediated vasculoprotective effect in the ApoE(-/-) mice, but aliskiren showed no further benefits in ApoE(-/-) CatS(-/-) mice. In vitro, TLR2 silencing reduced CatS expression induced by angiotensin II. Moreover, aliskiren or the inhibition of CatS impaired the endothelial cell angiogenic action in vitro or/and ex vivo.Renin inhibition appears to inhibit advanced plaque neovessel formation in ApoE(-/-) mice and to decrease the vascular inflammatory action and extracellular matrix degradation, partly by reducing AT1R/TLR2-mediated CatS activation and activity, thus regressing advanced atherosclerosis.
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- 2014
190. The tick salivary protein sialostatin L2 inhibits caspase-1-mediated inflammation during Anaplasma phagocytophilum infection
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John F. Andersen, Alicia K. Olivier, Xiaowei Wang, Eric J. Sundberg, Tyler K. Ulland, Joao H. F. Pedra, Michail Kotsyfakis, Guo-Ping Shi, Fayyaz S. Sutterwala, Maiara S. Severo, Mohammad Sohail, Olivia S. Sakhon, Yi Zhou, Mayukh Sircar, Gang Chen, Greg A. Snyder, and Lindsey J. Brown
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Saliva ,Inflammasomes ,Immunology ,Caspase 1 ,Inflammation ,Microbiology ,Cathepsin L ,Mice ,medicine ,Animals ,Pathogen ,Cells, Cultured ,Analysis of Variance ,Host Response and Inflammation ,NADPH oxidase ,biology ,Macrophages ,Ehrlichiosis ,Inflammasome ,biology.organism_classification ,bacterial infections and mycoses ,Anaplasma phagocytophilum ,Mice, Inbred C57BL ,Disease Models, Animal ,Infectious Diseases ,biology.protein ,Cytokines ,Salivary Cystatins ,Parasitology ,medicine.symptom ,Reactive Oxygen Species ,medicine.drug - Abstract
Saliva from arthropod vectors facilitates blood feeding by altering host inflammation. Whether arthropod saliva counters inflammasome signaling, a protein scaffold that regulates the activity of caspase-1 and cleavage of interleukin-1β (IL-1β) and IL-18 into mature molecules, remains elusive. In this study, we provide evidence that a tick salivary protein, sialostatin L2, inhibits inflammasome formation during pathogen infection. We show that sialostatin L2 targets caspase-1 activity during host stimulation with the rickettsial agent Anaplasma phagocytophilum . A. phagocytophilum causes macrophage activation and hemophagocytic syndrome features. The effect of sialostatin L2 in macrophages was not due to direct caspase-1 enzymatic inhibition, and it did not rely on nuclear factor κB or cathepsin L signaling. Reactive oxygen species from NADPH oxidase and the Loop2 domain of sialostatin L2 were important for the regulatory process. Altogether, our data expand the knowledge of immunoregulatory pathways of tick salivary proteins and unveil an important finding in inflammasome biology.
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- 2014
191. Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia
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Xian Wu Cheng, Haizhen Song, Yoshiharu Oshida, Haiying Jiang, Masafumi Kuzuya, Aiko Inoue, Hongxian Wu, Yumiko Yamamura, Xiang Li, Chang-Ning Hao, Lina Hu, Teruhiro Koike, Kyosuke Takeshita, Zhe Huang, Masashi Asai, Kenji Okumura, Guo-Ping Shi, Kazumasa Unno, and Toyoaki Murohara
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Vascular Endothelial Growth Factor A ,Cathepsin K ,Notch signaling pathway ,General Physics and Astronomy ,Cathepsin D ,Neovascularization, Physiologic ,Cell Cycle Proteins ,General Biochemistry, Genetics and Molecular Biology ,Cathepsin B ,Neovascularization ,Mice ,Ischemia ,Cathepsin L1 ,medicine ,Basic Helix-Loop-Helix Transcription Factors ,Laser-Doppler Flowmetry ,Animals ,RNA, Messenger ,Phosphorylation ,Receptor, Notch1 ,Hypoxia ,Muscle, Skeletal ,Ligation ,Cathepsin S ,Cathepsin ,Homeodomain Proteins ,Mice, Knockout ,Multidisciplinary ,Vascular Endothelial Growth Factor Receptor-1 ,Chemistry ,Reverse Transcriptase Polymerase Chain Reaction ,General Chemistry ,Molecular biology ,Capillaries ,Hindlimb ,Femoral Artery ,Repressor Proteins ,Transcription Factor HES-1 ,sense organs ,medicine.symptom ,Proto-Oncogene Proteins c-akt - Abstract
Cysteine proteases play important roles in pathobiology. Here we reveal that cathepsin K (CatK) has a role in ischaemia-induced neovascularization. Femoral artery ligation-induced ischaemia in mice increases CatK expression and activity, and CatK-deficient mice show impaired functional recovery following hindlimb ischaemia. CatK deficiency reduces the levels of cleaved Notch1 (c-Notch1), Hes1 Hey1, Hey2, vascular endothelial growth factor, Flt-1 and phospho-Akt proteins of the ischaemic muscles. In endothelial cells, silencing of CatK mimicked, whereas CatK overexpression enhanced, the levels of c-Notch1 and the expression of Notch downstream signalling molecules, suggesting CatK contributes to Notch1 processing and activates downstream signalling. Moreover, CatK knockdown leads to defective endothelial cell invasion, proliferation and tube formation, and CatK deficiency is associated with decreased circulating endothelial progenitor cells-like CD31(+)/c-Kit(+) cells in mice following hindlimb ischaemia. Transplantation of bone marrow-derived mononuclear cells from CatK(+/+) mice restores the impairment of neovascularization in CatK(-/-) mice. We conclude that CatK may be a potential therapeutic target for ischaemic disease.
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- 2014
192. Mechanisms with clinical implications for atrial fibrillation-associated remodeling: cathepsin K expression, regulation, and therapeutic target and biomarker
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Guo-Ping Shi, Toyoaki Murohara, Masayuki Shimano, Aiko Inoue, Seifuku Kyo, Masaya Fujita, Xian Wu Cheng, Yasuya Inden, Kyosuke Takeshita, Toshihiko Yamamoto, Kenji Okumura, Naoki Yoshida, Masafumi Kuzuya, and Noriko Taguchi
- Subjects
Male ,Phosphopeptides ,Cathepsin K ,p38 Mitogen-Activated Protein Kinases ,angiotensin type 1 receptor ,Fibrosis ,Superoxides ,Atrial Fibrillation ,Myocytes, Cardiac ,Arrhythmia and Electrophysiology ,Phosphorylation ,Receptor ,Cells, Cultured ,Original Research ,Angiotensin II ,Cardiac Pacing, Artificial ,Middle Aged ,Receptor antagonist ,Up-Regulation ,Female ,Rabbits ,Cardiology and Cardiovascular Medicine ,Procollagen ,Signal Transduction ,Adult ,medicine.medical_specialty ,medicine.drug_class ,extracellular matrix ,Receptor, Angiotensin, Type 1 ,N-terminal telopeptide ,Internal medicine ,Renin–angiotensin system ,medicine ,Animals ,Humans ,mitogen‐activated protein kinase ,Heart Atria ,Rats, Wistar ,Protein Kinase Inhibitors ,Aged ,business.industry ,NADPH Oxidases ,medicine.disease ,Rats ,Procollagen peptidase ,Disease Models, Animal ,Endocrinology ,Animals, Newborn ,Case-Control Studies ,business ,Angiotensin II Type 1 Receptor Blockers ,Biomarkers - Abstract
Background The cysteine protease cathepsin K (CatK) has been implicated in the pathogenesis of cardiovascular disease. We sought to determine the link between atrial fibrillation ( AF ) and plasma CatK levels and to investigate the expression of and therapeutic target for CatK in vivo and in vitro. Methods and Results Plasma CatK and extracellular matrix protein peptides (intact procollagen type I of N‐terminal propeptide; carboxyl‐terminal telopeptide of type I collagen [ ICTP ]) were measured in 209 consecutive patients with AF (paroxysmal AF , 146; persistent AF , 63) and 112 control subjects. In addition, the regulation of CatK expression was investigated in vivo and vitro. Patients with AF had higher plasma CatK and ICTP levels than did control subjects. Patients with persistent AF had higher levels of plasma CatK and ICTP than did patients with paroxysmal AF . CatK was correlated with ICTP concentration and left atrial diameter in all subjects. In rabbits, superoxide production, CatK activity, fibrosis, and the levels of atrial tissue angiotensin II , angiotensin type 1 receptor, gp91phox, phospho‐p38 mitogen‐activated protein kinase, and CatK were greater in those with tachypacing‐induced AF than in controls, and these changes were reversed with angiotensin type 1 receptor antagonist. Olmesartan and mitogen‐activated protein kinase inhibitor decreased the CatK expression induced by angiotensin II in rat neonatal myocytes. Conclusions These data indicated that increased plasma CatK levels are linked with the presence of AF . Angiotensin type 1 receptor antagonist appears to be effective in alleviating atrial fibrosis in a rabbit AF model, partly reducing angiotensin type 1 receptor‐p38mitogen‐activated protein kinase‐dependent and ‐independent CatK activation, thus preventing AF.
- Published
- 2013
193. Cathepsin K Activity Controls Injury-Related Vascular Repair in Mice
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Guo-Ping Shi, Masafumi Kuzuya, Eiji Kozawa, Lina Hu, Xian Wu Cheng, Haiying Jiang, Aiko Inoue, Kenji Okumura, Xiang Li, and Haizhen Song
- Subjects
Male ,medicine.medical_specialty ,Chemokine ,Pathology ,Cathepsin K ,Inflammation ,Article ,Muscle, Smooth, Vascular ,Lesion ,Mice ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Cell Proliferation ,Neointimal hyperplasia ,Mice, Knockout ,biology ,Cell growth ,Monocyte ,medicine.disease ,Disease Models, Animal ,medicine.anatomical_structure ,Endocrinology ,Collagenase ,biology.protein ,medicine.symptom ,Carotid Artery Injuries ,medicine.drug - Abstract
Cathepsin K (CatK) is one of the most potent mammalian collagenases. We showed previously the increased expression of CatK in human and animal atherosclerotic lesions. Here, we hypothesized that ablation of CatK mitigates injury-induced neointimal hyperplasia. Male wild-type (CatK +/+ ) and CatK-deficient (CatK −/− ) mice underwent ligation or a combination of ligation and polyethylene cuff-replacement injuries to the right common carotid artery just proximal to its bifurcation, and they were then processed for morphological and biochemical studies at specific time points. On operative day 28, CatK −/− significantly reduced neointimal formation and neovessel formation in both single- and combination-injured arteries compared with the Cat K +/+ mice. At early time points, CatK −/− reduced the lesion macrophage contents and medial smooth muscle cell proliferation, the mRNA levels of monocyte chemoattractant protein-1, toll-like receptor-2, toll-like receptor-4, chemokine ligand-12, and the gelatinolytic activity related to matrix metalloproteinase-2/-9. An aorta-explant assay revealed that smooth muscle cell movement was impaired in the CatK −/− mice compared with the CatK +/+ mice. In addition, the smooth muscle cells and macrophages from CatK −/− mice had less invasive ability through a reconstituted basement membrane barrier. This vasculoprotective effect was mimicked by Cat inhibition with trans -epoxysuccinyl-L-leucylamido-{4-guanidino} butane (E64 d ). These results demonstrate an essential role of CatK in neointimal lesion formation in response to injury, possibly via the reduction of toll-like receptor-2/-4–mediated inflammation and smooth muscle cell proliferation, suggesting a novel therapeutic strategy for the control of endovascular treatment–related restenosis by regulating CatK activity.
- Published
- 2013
194. Pathologic gene expression in Gaucher disease: up-regulation of cysteine proteinases including osteoclastic cathepsin K
- Author
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Alison R. Hayman, J. Paul Schofield, Guo-Ping Shi, Mary Teresa Moran, Elisabeth Young, and Timothy M. Cox
- Subjects
Cathepsin ,Immunology ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Molecular biology ,Cathepsin B ,Cathepsin C ,Cathepsin O ,Cathepsin H ,Cathepsin L1 ,Cathepsin K ,Cathepsin S - Abstract
Deficiency of lysosomal acid β-glucosidase induces glycolipid storage in the macrophages of Gaucher disease but the pathways of multisystem tissue injury and destruction are unknown. To investigate the cognate molecular pathology of this inflammatory disorder, genes that were differentially expressed in spleen samples from a patient with Gaucher disease (Gaucher spleen) were isolated. Of 64 complementary DNA (cDNA) fragments sequenced from an enriched Gaucher cDNA library, 5 encode lysosomal proteins (cathepsins B, K, and S, α-fucosidase, and acid lipase), 10 encode other known proteins, and 2 represent novel sequences from human macrophage cell lines. Transcript abundance of the cathepsins, novel genes, pulmonary and activation-regulated chemokine (PARC), and NMB, a putative tumor suppressor gene, was greatly increased. Immunoblotting showed increased mature forms of all 3 cathepsins found in samples of Gaucher spleens. Immunofluorescence microscopy showed strong cathepsin B and K reactions in sinusoidal endothelium and Gaucher cells. The respective means, plus or minus SD, of cathepsin B, K, and S activities were 183 ± 35, 97 ± 39, and 91 ± 45 nmol/min/mg protein in 4 Gaucher spleens, and 26 ± 4, 10.5 ± 2, and 4.0 ± 2.1 nmol/min/mg protein in 3 control spleens. Plasma cathepsin B, K, and S activities were also elevated in Gaucher disease plasma (P
- Published
- 2000
195. Pathologic gene expression in Gaucher disease: up-regulation of cysteine proteinases including osteoclastic cathepsin K
- Author
-
Mary Teresa Moran, J. Paul Schofield, Alison R. Hayman, Guo-Ping Shi, Elisabeth Young, and Timothy M. Cox
- Subjects
Immunology ,Cell Biology ,Hematology ,Biochemistry - Abstract
Deficiency of lysosomal acid β-glucosidase induces glycolipid storage in the macrophages of Gaucher disease but the pathways of multisystem tissue injury and destruction are unknown. To investigate the cognate molecular pathology of this inflammatory disorder, genes that were differentially expressed in spleen samples from a patient with Gaucher disease (Gaucher spleen) were isolated. Of 64 complementary DNA (cDNA) fragments sequenced from an enriched Gaucher cDNA library, 5 encode lysosomal proteins (cathepsins B, K, and S, α-fucosidase, and acid lipase), 10 encode other known proteins, and 2 represent novel sequences from human macrophage cell lines. Transcript abundance of the cathepsins, novel genes, pulmonary and activation-regulated chemokine (PARC), and NMB, a putative tumor suppressor gene, was greatly increased. Immunoblotting showed increased mature forms of all 3 cathepsins found in samples of Gaucher spleens. Immunofluorescence microscopy showed strong cathepsin B and K reactions in sinusoidal endothelium and Gaucher cells. The respective means, plus or minus SD, of cathepsin B, K, and S activities were 183 ± 35, 97 ± 39, and 91 ± 45 nmol/min/mg protein in 4 Gaucher spleens, and 26 ± 4, 10.5 ± 2, and 4.0 ± 2.1 nmol/min/mg protein in 3 control spleens. Plasma cathepsin B, K, and S activities were also elevated in Gaucher disease plasma (P
- Published
- 2000
196. Simple Modifications of the Serpin Reactive Site Loop Convert SCCA2 into a Cysteine Proteinase Inhibitor: A Critical Role for the P3‘ Proline in Facilitating RSL Cleavage
- Author
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Guo-Ping Shi, Gary A. Silverman, Charles Schick, Dieter Brömme, Christopher Tsu, James C. Whisstock, Harold A. Chapman, Cliff J. Luke, Luiz Juliano, and James A. Irving
- Subjects
Models, Molecular ,Proline ,Molecular Sequence Data ,Mutant ,Sequence alignment ,Serpin ,Biochemistry ,Antigens, Neoplasm ,Enzyme Stability ,Papain ,Humans ,Amino Acid Sequence ,Binding site ,Peptide sequence ,Serpins ,Cathepsin ,Binding Sites ,biology ,Active site ,Cathepsins ,Molecular biology ,Peptide Fragments ,Elastin ,Kinetics ,Mutagenesis, Site-Directed ,biology.protein ,Sequence Alignment ,Cysteine - Abstract
The human squamous cell carcinoma antigens (SCCA) 1 and 2 are members of the serpin family that are 92% identical in their amino acid sequence. Despite this similarity, they inhibit distinct classes of proteinases. SCCA1 neutralizes the papain-like cysteine proteinases, cathepsins (cat) S, L, and K; and SCCA2 inhibits the chymotrypsin-like serine proteinases, catG and human mast cell chymase. SCCA2 also can inhibit catS, as well as other papain-like cysteine proteinases, albeit at a rate 50-fold less than that of SCCA1. Analysis of the mechanism of inhibition by SCCA1 revealed that the reactive site loop (RSL) is important for cysteine proteinase inhibition. The inhibition of catS by a mutant SCCA2 containing the RSL of SCCA1 is comparable to that of wild-type SCCA1. This finding suggested that there were no motifs outside and only eight residues within the RSL that were directing catS-specific inhibition. The purpose of this study was to determine which of these residues might account for the marked difference in the ability of SCCA1 and SCCA2 to inhibit papain-like cysteine proteinases. SCCA2 molecules containing different RSL mutations showed that no single amino acid substitution could convert SCCA2 into a more potent cysteine proteinase inhibitor. Rather, different combinations of mutations led to incremental increases in catS inhibitory activity with residues in four positions (P1, P3', P4', and P11') accounting for 80% of the difference in activity between SCCA1 and SCCA2. Interestingly, the RSL cleavage site differed between wild-type SCCA2 and this mutant. Moreover, these data established the importance of a Pro residue in the P3' position for efficient inhibition of catS by both wild-type SCCA1 and mutated SCCA2. Molecular modeling studies suggested that this residue might facilitate positioning of the RSL within the active site of the cysteine proteinase.
- Published
- 2000
197. Protease Injury in the Development of COPD
- Author
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Guo-Ping Shi and Harold A. Chapman
- Subjects
Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,COPD ,Protease ,business.industry ,medicine.medical_treatment ,medicine ,Physical therapy ,Cardiology and Cardiovascular Medicine ,Critical Care and Intensive Care Medicine ,Intensive care medicine ,business ,medicine.disease - Published
- 2000
198. Early endosomal maturation of MHC class II molecules independently of cysteine proteases and H-2DM
- Author
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Jose A Villadangos, Guo-Ping Shi, Christoph Driessen, Harold A. Chapman, and Hidde L. Ploegh
- Subjects
CD4-Positive T-Lymphocytes ,CD74 ,Endosome ,Antigen presentation ,Peptide binding ,Endosomes ,General Biochemistry, Genetics and Molecular Biology ,Mice ,Animals ,Molecular Biology ,Cathepsin S ,Antigen Presentation ,MHC class II ,General Immunology and Microbiology ,biology ,Antigen processing ,General Neuroscience ,H-2 Antigens ,Histocompatibility Antigens Class II ,Biological Transport ,Articles ,Cysteine protease ,Mice, Inbred C57BL ,Cysteine Endopeptidases ,Biochemistry ,biology.protein - Abstract
Major histocompatibility complex (MHC) class II molecules bind and present to CD4(+) T cells peptides derived from endocytosed antigens. Class II molecules associate in the endoplasmic reticulum with invariant chain (Ii), which (i) mediates the delivery of the class II-Ii complexes into the endocytic compartments where the antigenic peptides are generated; and (ii) blocks the peptide-binding site of the class II molecules until they reach their destination. Once there, Ii must be removed to allow peptide binding. The bulk of Ii-class II complexes reach late endocytic compartments where Ii is eliminated in a reaction in which the cysteine protease cathepsin S and the accessory molecule H-2DM play an essential role. Here, we here show that Ii is also eliminated in early endosomal compartments without the intervention of cysteine proteases or H-2DM. The Ii-free class II molecules generated by this alternative mechanism first bind high molecular weight polypeptides and then mature into peptide-loaded complexes.
- Published
- 2000
199. Identification of the increased expression of monocyte chemoattractant protein-1, cathepsin S, UPIX-1, and other genes in dystrophin-deficient mouse muscles by suppression subtractive hybridization
- Author
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Jin Fang, Pal L. Vaghy, and Guo-Ping Shi
- Subjects
Cathepsin ,mdx mouse ,biology ,Duchenne muscular dystrophy ,Cell Biology ,medicine.disease ,Biochemistry ,Molecular biology ,Cathepsin B ,Suppression subtractive hybridization ,biology.protein ,medicine ,Northern blot ,Dystrophin ,Molecular Biology ,Cathepsin S - Abstract
The lack of dystrophin results in muscular dystrophy characterized by degeneration, inflammation, and partial regeneration of skeletal muscles. The fate of these muscles may be determined by the extent of adaptation to the defect and the efficiency of regeneration that is affected by inflammatory cells. We have used suppression subtractive hybridization and quantitative Northern blot analysis to identify differentially expressed genes. Increased expression of murine monocyte chemoattractant protein-1 (JE/MCP-1), cathepsin S, UPIX-1, nmb, cathepsin B, and lysozyme M mRNAs were identified in 2-month-old mdx mouse leg muscles. UPIX-1 is a novel gene. Although it was not expressed in control muscles, it was expressed in control brain, heart, and spleen. JE/MCP-1 and cathepsin S proteins in mdx muscles, as well as JE/MCP-1 protein in the serum of mdx mice were also detected. JE/MCP-1 may be responsible for attraction of inflammatory cells, and cathepsin S, a potent elastolytic protease, may contribute to the remodeling of the extracellular matrix that is required for the migration of these cells to the injured muscles. J. Cell. Biochem. 79:164–172, 2000. © 2000 Wiley-Liss, Inc.
- Published
- 2000
200. Proteases involved in MHC dass II antigen presentation
- Author
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Ana-Maria Lennon-Duménil, Christoph Peters, Jan M. Deussing, Rebecca A.R. Bryant, Harold A. Chapman, Jose A Villadangos, Christoph Driessen, Hidde L. Ploegh, Richard J. Riese, Paul Saftig, Wera Roth, and Guo-Ping Shi
- Subjects
Proteases ,Cathepsin L ,Immunology ,Antigen presentation ,Biology ,Cathepsin D ,Models, Biological ,Cathepsin B ,MHC class II antigen ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Antigen ,Endopeptidases ,Animals ,Humans ,Immunology and Allergy ,030304 developmental biology ,Cathepsin S ,Antigen Presentation ,0303 health sciences ,Antigen processing ,Histocompatibility Antigens Class II ,Cell Differentiation ,Dendritic Cells ,Cathepsins ,Cysteine Endopeptidases ,Biochemistry ,030215 immunology - Abstract
Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. The study of genetically modified mice deficient in single lysosomal proteases has allowed us to determine their role in these processes. Cathepsins (Cat) B and D, previously considered major players in MHC class II antigen presentation, are dispensable for degradation of Ii and for generation of several antigenic determinants. By contrast, Cat S plays an essential role in removal of Ii in B cells and dendritic cells, whereas Cat L apparently does so in thymic epithelial cells. Accordingly, the absence of Cat S and L have major consequences for the onset of humoral immune responses and for T-cell selection, respectively. It is likely that other as yet uncharacterized lysosomal enzymes also play a role in Ii degradation and in generation of antigenic determinants. Experiments involving drugs that interfere with protein traffic suggest that more than one mechanism for Ii removal, probably involving different proteases, can co-exist in the same antigen-presenting cell. These findings may allow the development of protease inhibitors with possible therapeutic applications.
- Published
- 1999
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