151. YB-1 Is Important for an Early Stage Embryonic Development
- Author
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Takakazu Sasaguri, Kimitoshi Kohno, Hiroto Izumi, Takanori Nakamura, Michihiko Kuwano, Kohtaro Shibahara, Takeshi Uchiumi, Tatsuo Shimada, Takao Fukuda, Abbas Fotovati, and Teruhisa Tsuzuki
- Subjects
Cell growth ,Embryogenesis ,Neural tube ,Cell Biology ,Exencephaly ,Biology ,medicine.disease ,Biochemistry ,Embryonic stem cell ,Cell biology ,Neuroepithelial cell ,medicine.anatomical_structure ,Neurulation ,medicine ,Molecular Biology ,Abnormal cell morphology - Abstract
The eukaryotic Y-box-binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock-out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. beta-Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1(-/-) mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1(-/-) embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1(-/-) mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact-inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation.
- Published
- 2006