Search

Your search keyword '"Navarro, Ferran"' showing total 520 results
Start Over Author "Navarro, Ferran"
520 results on '"Navarro, Ferran"'

151. Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

Author

Ortega, Adriana, primary, Oteo, Jesús, additional, Aranzamendi-Zaldumbide, Maitane, additional, Bartolomé, Rosa M., additional, Bou, Germán, additional, Cercenado, Emilia, additional, Conejo, M. Carmen, additional, González-López, Juan José, additional, Marín, Mercedes, additional, Martínez-Martínez, Luis, additional, Merino, María, additional, Navarro, Ferran, additional, Oliver, Antonio, additional, Pascual, Álvaro, additional, Rivera, Alba, additional, Rodríguez-Baño, Jesús, additional, Weber, Irene, additional, Aracil, Belén, additional, and Campos, José, additional

Published
2012
Full Text
View/download PDF

152. Colonisation and infection due to Enterobacteriaceae producing plasmid-mediated AmpC β-lactamases

Author

Rodríguez-Baño, Jesús, primary, Miró, Elisenda, additional, Villar, Macarena, additional, Coelho, Alicia, additional, Gozalo, Mónica, additional, Borrell, Nuria, additional, Bou, Germán, additional, Conejo, M. Carmen, additional, Pomar, Virginia, additional, Aracil, Belén, additional, Larrosa, Nieves, additional, Agüero, Jesús, additional, Oliver, Antonio, additional, Fernández, Ana, additional, Oteo, Jesús, additional, Pascual, Alvaro, additional, and Navarro, Ferran, additional

Published
2012
Full Text
View/download PDF

154. Diagnostic accuracy of a 16S ribosomal DNA gene-based molecular technique (RT-PCR, microarray, and sequencing) for bacterial meningitis, early-onset neonatal sepsis, and spontaneous bacterial peritonitis

Author

Esparcia, Oscar, primary, Montemayor, Michel, additional, Ginovart, Gemma, additional, Pomar, Virginia, additional, Soriano, Germán, additional, Pericas, Roser, additional, Gurgui, Mercedes, additional, Sulleiro, Elena, additional, Prats, Guillem, additional, Navarro, Ferran, additional, and Coll, Pere, additional

Published
2011
Full Text
View/download PDF

155. Corrigendum to “Extended-spectrum β-lactamase-producing Escherichia coli in Spain belong to a large variety of multilocus sequence typing types, including ST10 complex/A, ST23 complex/A and ST131/B2” [Int. J. Antimicrob. Agents 34 (2009) 173–176]

Author

Oteo, Jesús, primary, Diestra, Karol, additional, Juan, Carlos, additional, Bautista, Verónica, additional, Novais, Ângela, additional, Pérez-Vázquez, María, additional, Moyá, Bartolome, additional, Miró, Elisenda, additional, Coque, Teresa M., additional, Oliver, Antonio, additional, Cantón, Rafael, additional, Navarro, Ferran, additional, and Campos, José, additional

Published
2010
Full Text
View/download PDF

156. Fe de errores de «Caracterización y epidemiología molecular de betalactamasas de espectro extendido en Escherichia coli y Klebsiella pneumoniae en once hospitales españoles (2004)»

Author

Diestra, Karol, primary, Coque, Teresa M., additional, Miró, Elisenda, additional, Oteo, Jesús, additional, Juan Nicolau, Carlos, additional, Campos, José, additional, Moyá, Bartolomé, additional, Curiao, Tânia, additional, Pérez-Vázquez, María, additional, Cantón, Rafael, additional, Oliver, Antonio, additional, and Navarro, Ferran, additional

Published
2010
Full Text
View/download PDF

158. Extended-spectrum β-lactamase-producing Escherichia coli in Spain belong to a large variety of multilocus sequence typing types, including ST10 complex/A, ST23 complex/A and ST131/B2

Author

Oteo, Jesús, primary, Diestra, Karol, additional, Juan, Carlos, additional, Bautista, Verónica, additional, Novais, Ângela, additional, Pérez-Vázquez, María, additional, Moyá, Bartolome, additional, Miró, Elisenda, additional, Coque, Teresa M., additional, Oliver, Antonio, additional, Cantón, Rafael, additional, Navarro, Ferran, additional, and Campos, José, additional

Published
2009
Full Text
View/download PDF

159. Caracterización y epidemiología molecular de betalactamasas de espectro extendido en Escherichia coli y Klebsiella pneumoniae en once hospitales españoles (2004)

Author

Diestra, Karol, primary, Coque, Teresa M., additional, Miró, Elisenda, additional, Oteo, Jesús, additional, Juan Nicolau, Carlos, additional, Campos, José, additional, Moyá, Bartolomé, additional, Curiao, Tânia, additional, Pérez-Vázquez, María, additional, Cantón, Rafael, additional, Oliver, Antonio, additional, and Navarro, Ferran, additional

Published
2008
Full Text
View/download PDF

163. Recomendaciones para la selección de antimicrobianos en el estudio de la sensibilidad in vitro con sistemas automáticos y semiautomáticos

Author

Cantón, Rafael, primary, Ignacio Alós, Juan, additional, Baquero, Fernando, additional, Calvo, Jorge, additional, Campos, José, additional, Castillo, Javier, additional, Cercenado, Emilia, additional, Ángeles Domínguez, M., additional, Liñares, Josefina, additional, López-Cerezo, Lorena, additional, Marco, Francesc, additional, Mirelis, Beatriz, additional, Morosini, María-Isabel, additional, Navarro, Ferran, additional, Oliver, Antonio, additional, Pérez-Trallero, Emilio, additional, Torres, Carmen, additional, and Martínez-Martínez, Luis, additional

Published
2007
Full Text
View/download PDF

165. Metallo-β-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues

Author

Cornaglia, Giuseppe, primary, Akova, Murat, additional, Amicosante, Gianfranco, additional, Cantón, Rafael, additional, Cauda, Roberto, additional, Docquier, Jean-Denis, additional, Edelstein, Mikhail, additional, Frère, Jean-Marie, additional, Fuzi, Miklós, additional, Galleni, Moreno, additional, Giamarellou, Helen, additional, Gniadkowski, Marek, additional, Koncan, Raffaella, additional, Libisch, Balázs, additional, Luzzaro, Francesco, additional, Miriagou, Vivi, additional, Navarro, Ferran, additional, Nordmann, Patrice, additional, Pagani, Laura, additional, Peixe, Luisa, additional, Poirel, Laurent, additional, Souli, Maria, additional, Tacconelli, Evelina, additional, Vatopoulos, Alkiviadis, additional, and Rossolini, Gian Maria, additional

Published
2007
Full Text
View/download PDF

184. Prospective Multicenter Study of Carbapenemase-Producing Enterobacteriaceaefrom 83 Hospitals in Spain Reveals High In VitroSusceptibility to Colistin and Meropenem

Author

Oteo, Jesús, Ortega, Adriana, Bartolomé, Rosa, Bou, Germán, Conejo, Carmen, Fernández-Martínez, Marta, González-López, Juan José, Martínez-García, Laura, Martínez-Martínez, Luis, Merino, María, Miró, Elisenda, Mora, Marta, Navarro, Ferran, Oliver, Antonio, Pascual, Álvaro, Rodríguez-Baño, Jesús, Ruiz-Carrascoso, Guillermo, Ruiz-Garbajosa, Patricia, Zamorano, Laura, Bautista, Verónica, Pérez-Vázquez, María, and Campos, José

Abstract

ABSTRACTThe aim of this study was to determine the impact of carbapenemase-producing Enterobacteriaceae(CPE) in Spain in 2013 by describing the prevalence, dissemination, and geographic distribution of CPE clones, and their population structure and antibiotic susceptibility. From February 2013 to May 2013, 83 hospitals (about 40,000 hospital beds) prospectively collected nonduplicate Enterobacteriaceaeusing the screening cutoff recommended by EUCAST. Carbapenemase characterization was performed by phenotypic methods and confirmed by PCR and sequencing. Multilocus sequencing types (MLST) were determined for Klebsiella pneumoniaeand Escherichia coli. A total of 702 Enterobacteriaceaeisolates met the inclusion criteria; 379 (54%) were CPE. OXA-48 (71.5%) and VIM-1 (25.3%) were the most frequent carbapenemases, and K. pneumoniae(74.4%), Enterobacter cloacae(10.3%), and E. coli(8.4%) were the species most affected. Susceptibility to colistin, amikacin, and meropenem was 95.5%, 81.3%, and 74.7%, respectively. The most prevalent sequence types (STs) were ST11 and ST405 for K. pneumoniaeand ST131 for E. coli. Forty-five (54.1%) of the hospitals had at least one CPE case. For K. pneumoniae, ST11/OXA-48, ST15/OXA-48, ST405/OXA-48, and ST11/VIM-1 were detected in two or more Spanish provinces. ST11 isolates carried four carbapenemases (VIM-1, OXA-48, KPC-2, and OXA-245), but ST405 isolates carried OXA-48 only. A wide interregional spread of CPE in Spain was observed, mainly due to a few successful clones of OXA-48-producing K. pneumoniae(e.g., ST11 and ST405). The dissemination of OXA-48-producing E. coliis a new finding of public health concern. According to the susceptibilities determined in vitro, most of the CPE (94.5%) had three or more options for antibiotic treatment.

Published
2015
Full Text
View/download PDF

185. Evolution of carbapenemase-producing Enterobacteriaceae at the global and national level: What should be expected in the future?

Author

Oteo, Jesús, Miró, Elisenda, Pérez-Vázquez, María, and Navarro, Ferran

Abstract

In recent years, Enterobacteriaceae isolates have increased their potential to become highly drug resistant by acquiring resistance to carbapenems, primarily due to the production of acquired carbapenemases. The carbapenemases detected in Enterobacteriaceae are largely of the KPC, VIM, NDM, IMP and OXA-48 types. Although the epidemiological origin and geographic distribution of carbapenemases are clearly different, they all first appeared in the late 20th Century. Only a decade later, these enzymes have already become established and have expanded globally.

Published
2014
Full Text
View/download PDF

187. Prospective study of bacteremia during transesophageal echocardiography

Author

Lamich, Rubén, primary, Alonso, Carles, additional, Gumá, Joan R, additional, Ramirez, Inma, additional, García-Moll, Xavier, additional, Mirelis, Beatriz, additional, Navarro, Ferran, additional, Prats, Guillem, additional, Borrás, Xavier, additional, Carreras, Francesc, additional, and Pons-Llado, Guillem, additional

Published
1993
Full Text
View/download PDF

189. Plasmid typing and genetic context of AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: findings from a Spanish hospital 1999–2007.

Author

Mata, Caterina, Miró, Elisenda, Alvarado, Andrés, Garcillán-Barcia, M. Pilar, Toleman, Mark, Walsh, Timothy R., de la Cruz, Fernando, and Navarro, Ferran

Subjects
PLASMID genetics, BETA lactamases, ENTEROBACTERIACEAE, MOBILE genetic elements, TRANSPOSONS
Abstract

Objectives To gain insights into ampC transmission between bacterial strains. Methods We examined the genetic context of 117 acquired ampC genes from 27119 Enterobacteriaceae collected between 1999 and 2007. Plasmid analysis was carried out by PCR-based replicon or relaxase typing, S1-PFGE and Southern hybridization. I-CeuI/PFGE was used for isolates not characterized by plasmid analysis. PCR reactions were used to map the genetic organization of the ampC genes. Results Among the isolates studied, 81.2% of ampC genes were located on plasmids of known Inc/MOB groups, 7.7% were chromosomally located and 11.1% were not determined. A/C, I1 and K were the most commonly found replicons in plasmids carrying blaCMY-2, while L/M replicons were associated with blaDHA-1. blaACC-1 was linked to I1 and MOBF11 plasmids; blaCMY-27 was associated with IncF and MOBP12 plasmids; the plasmid carrying blaCMY-25 could not be typed, and blaCMY-40 was chromosomally located. All 87 isolates carrying blaCMY-2, blaCMY-4, blaCMY-25, blaCMY-27, blaCMY-40 or blaACC-1 displayed the transposon-like structures ISEcp1/ΔISEcp1-blaCMY-blc-sugE or ΔISEcp1-blaACC-1-gdha. The most prevalent structure in blaDHA-1 (93.3% of cases) was identical to that described in the Klebsiella pneumoniae pTN60013 plasmid. Remarkably, in three isolates containing chromosomal blaCMY-2, this gene was mobilized by conjugation. Conclusions Although plasmids are the main cause of the rapid dissemination of ampC genes among bacteria, we need to be aware that other mobile genetic elements such as integrative and conjugative elements (ICEs) can be involved in the mobilization of these genes. [ABSTRACT FROM PUBLISHER]

Published
2012
Full Text
View/download PDF

190. Prevalence of SXT/R391-like integrative and conjugative elements carrying blaCMY-2 in Proteus mirabilis.

Author

Mata, Caterina, Navarro, Ferran, Miró, Elisenda, Walsh, Timothy R., Mirelis, Beatriz, and Toleman, Mark

Subjects
*BETA lactamases, *PLASMIDS, *MOLECULAR genetics, *MIRABILIS, *GENES, *SOUTHERN blot
Abstract

Objectives To characterize the vectors involved in the dissemination of blaCMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. Methods Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could not be characterized were examined for the presence of SXT/R391-like elements. To demonstrate the involvement of these elements in the dissemination of blaCMY-2, we performed a PCR amplification of the integrase (int) and toxin/antitoxin (TA) genes from SXT/R391-like integrative conjugative elements (ICEs). Later on, I-Ceu-I PFGE gels and hybridization with blaCMY-2, int and prfC probes were performed. The genetic organization of blaCMY-2 was also studied. Results ampC genes were located on large conjugative plasmids in 11 of the 19 (58%) P. mirabilis studied. However, in eight of these isolates a plasmid was not involved in the mobilization of ampC genes. I-Ceu-I PFGE and hybridization analyses revealed that blaCMY-2 were chromosomally located in these eight P. mirabilis isolates. The genetic organization of blaCMY-2 and hybridization analyses revealed that blaCMY-2 was carried by an ICE almost identical to ICEPmiJpan1 in seven out of these eight isolates. Conclusions The prevalence of ICEs carrying blaCMY-2 was surprisingly high [37% (7 out of 19)]. This is the first study giving prevalence data on ICEs carrying blaCMY-2 genes. These results suggest the need to study these mobile genetic elements in the context of dissemination of acquired AmpC β-lactamases and also of other β-lactamases, such as extended-spectrum β-lactamases and carbapenemases. [ABSTRACT FROM PUBLISHER]

Published
2011
Full Text
View/download PDF

191. In vivo transmission of a plasmid coharbouring blaDHA-1 and qnrB genes between Escherichia coli and Serratia marcescens.

Author

Mata, Caterina, Miró, Elisenda, Mirelis, Beatriz, Garcillán-Barcia, Maria Pilar, de la Cruz, Fernando, Coll, Pere, and Navarro, Ferran

Subjects
PLASMIDS, SERRATIA marcescens, CYTOPLASMIC inheritance, ENTEROBACTERIACEAE, ESCHERICHIA coli, POLYMERASE chain reaction, SOUTHERN blot, QUINOLONE antibacterial agents, ANTIBACTERIAL agents
Abstract

We report a Serratia marcescens and an Escherichia coli isolate simultaneously detected in the same patient. Both isolates showed susceptibility patterns suggestive of harbouring a plasmid-mediated AmpC β-lactamase (pACBL) and a plasmid-encoded quinolone resistance (PMQR). PCR-based replicon, MOB typing, plasmid profile and Southern hybridization analyses revealed that both isolates coharboured blaDHA-1 and qnrB genes on the same IncL/M-MOBP13 plasmid approximately 70 kb in size. Together with the fact that both plasmids were conjugative in the laboratory, these results strongly suggest that a horizontal transfer event could take place in vivo. This is the first report of an isolate of S. marcescens harbouring a pACBL. The only phenotypic method that suggests the presence of a pACBL in an isolate harbouring an inducible chromosomal AmpC enzyme is the observation of scattered colonies near the edge of the inhibition zones of some β-lactams. The presence of both resistance genes on the same plasmid and the reported increase in PMQR could perhaps explain the widespread distribution of blaDHA-1 genes. [ABSTRACT FROM AUTHOR]

Published
2010
Full Text
View/download PDF

192. Evidence for convergent evolution of CTX-M-14 ESBL in Escherichia coli and its prevalence.

Author

Navarro, Ferran, Mesa, Rául-Jesús, Mir, Elisenda, Gómez, Laura, Mirelis, Beatriz, and Coll, Pere

Subjects
*ESCHERICHIA coli, *BETA lactamases, *GENES, *NUCLEOTIDES
Abstract

A total of 2440 Escherichia coli strains isolated in 2003 at the Hospital de la Santa Creu i Sant Pau were evaluated for the presence of extended-spectrum β-lactamases. Two different nucleotide sequences that encode the same β-lactamase, CTX-M-14, were detected when the blaCTX-M-14-genes of 35 E. coli isolates were analysed. Thirty-two of the 35 had the previously described sequence of the blaCTX-M-14 ( ), named blaCTX-M-14a, and the remaining three isolates showed a nucleotide sequence identical to that of the blaCTX-M-9 gene except for one nucleotide, named blaCTX-M-14b. Characterisation of the regions surrounding the blaCTX-M-14a showed the IS Ecp1 and the IS 903 upstream and downstream, respectively, of the bla gene, whereas the regions surrounding the blaCTX-M-14b contained the genetic environment described for the blaCTX-M-9 gene, the In 60. Characterisation by hybridisation showed that the blaCTX-M-14a was present in IncK plasmids, whereas the blaCTX-M-14b was found in the HI2 Inc group. The CTX-M-14 ESBL in E. coli isolates is the result of the convergence of two different genes. [ABSTRACT FROM AUTHOR]

Published
2007
Full Text
View/download PDF

193. Acquisition and diffusion of blaCTX-M-9 gene by R478-IncHI2 derivative plasmids.

Author

García, Aurora, Navarro, Ferran, Mir, Elisenda, Villa, Laura, Mirelis, Beatriz, Coll, Pere, and Carattoli, Alessandra

Subjects
*ESCHERICHIA coli, *PLASMIDS, *SALMONELLA, *GENETIC vectors, *FOOD pathogens
Abstract

Escherichia coli and Salmonella enterica isolates carrying the blaCTX-M-9 gene located on plasmids prevailed at the Hospital de la Santa Creu i Sant Pau, Barcelona, Spain in the 1996–1999 period. The blaCTX-M-9-carrying plasmids showed a great variability in size, suggesting the mobilization of the gene among different plasmid scaffolds. The aim of the present work was to identify and better characterize the plasmids involved in the spread of the blaCTX-M-9 gene. Results showed that the majority of these strains carried plasmids belonging to the IncHI2 incompatibility group. The IncHI2 plasmids were further characterized, and found to be related to the reference IncHI2 plasmid R478. [ABSTRACT FROM AUTHOR]

Published
2007
Full Text
View/download PDF

194. Cephalosporin-resistant Escherichia coli among summer camp attendees with salmonellosis.

Author

Prats, Guillem, Mirelis, Beatriz, Miro, Elisenda, Navarro, Ferran, Llovet, Teresa, Johnson, James R., Camps, Neus, Dominguez, Angela, Salleras, Lluis, Miró, Elisenda, Domínguez, Angela, and Salleras, Lluís

Subjects
FOOD poisoning, SALMONELLA, GASTROENTERITIS, GASTROINTESTINAL diseases, PLASMIDS
Abstract

Investigation of an acute gastroenteritis outbreak involving >100 persons at a summer camp in Girona, Spain, in June 2002 led to the detection of Salmonella enterica and extended-spectrum cephalosporin-resistant Escherichia coil (ESCREC). Stool cultures were performed for 22 symptomatic campers, three asymptomatic food handlers, and 10 healthy household members. Of the 22 campers, 19 had Salmonella enterica, 9 had an ESCREC strain carrying an extended-spectrum β-lactamase, and 2 had a second ESCREC strain carrying a plasmidic cephamycinase. Related ESCREC were detected in two (salmonella-negative) asymptomatic food handlers and in none of the healthy household members. Fecal ESCREC and its β-lactamases and plasmids were extensively characterized. Three of the five ESCREC clones were recovered from multiple hosts. The apparent dissemination of ESCREC suggests a food or water vehicle. The observed distribution of resistance plasmids and β-lactamase genes in several clones indicates a high degree of horizontal transfer. Heightened vigilance and increased efforts must be made to discover the reservoirs and vehicles for community dissemination of ESCREC. [ABSTRACT FROM AUTHOR]

Published
2003
Full Text
View/download PDF

195. Beta-lactamases involved in resistance to broad-spectrum cephalosporins in Escherichia coli and Klebsiella spp. clinical isolates collected between 1994 and 1996, in Barcelona (Spain).

Author

Sabaté, Montserrat, Miró, Elisenda, Navarro, Ferran, Vergés, Clara, Aliaga, Roxana, Mirelis, Beatriz, and Prats, Guillermo

Abstract

The aim of this study was to evaluate the incidence of decreased susceptibility to broad-spectrum cephalosporins in Enterobacteriaceae that lack inducible chromosomal bla genes, and to determine the enzymes responsible for resistance. From all clinically relevant Enterobacteriaceae strains isolated between 1994 and 1996, 88 of 7054 Escherichia coli, seven of 581 Klebsiella pneumoniae and 23 of 166 Klebsiella oxytoca strains were studied because of their decreased susceptibilities to broad-spectrum cephalosporins (as reflected in intermediate susceptibilities and/or positive synergy tests and/or irregular crenellated inhibition zones). The most frequent mechanism implicated in decreased susceptibility to broad-spectrum cephalosporins displayed by E. coli and K. oxytoca was hyperproduction of chromosomal beta-lactamase, followed by plasmid-mediated SHV-1 hyperproduction in E. coli. In our hospital, the incidence of plasmid-mediated extended-spectrum beta-lactamases (ESBLs) between 1994 and 1996 was low. ESBLs were found in only 10 (0.14%) E. coli strains (six CTX-M-9, two TEM-12 and two SHV-2), in one (0.17%) K. pneumoniae strain (SHV-2) and in no K. oxytoca strains. The relatively wide variety of beta-lactamases that were detected among these common bacteria isolated from a single medical centre, including non-TEM- and non-SHV-derived ESBLs, appears epidemiologically remarkable. [ABSTRACT FROM AUTHOR]

Published
2002
Full Text
View/download PDF

197. Inhibitor-Resistant TEM- and OXA-1-Producing Escherichia coliIsolates Resistant to Amoxicillin-Clavulanate Are More Clonal and Possess Lower Virulence Gene Content than Susceptible Clinical Isolates

Author

Oteo, Jesús, González-López, Juan José, Ortega, Adriana, Quintero-Zárate, J. Natalia, Bou, Germán, Cercenado, Emilia, Conejo, María Carmen, Martínez-Martínez, Luis, Navarro, Ferran, Oliver, Antonio, Bartolomé, Rosa M., and Campos, José

Abstract

ABSTRACTIn a previous prospective multicenter study in Spain, we found that OXA-1 and inhibitor-resistant TEM (IRT) β-lactamases constitute the most common plasmid-borne mechanisms of genuine amoxicillin-clavulanate (AMC) resistance in Escherichia coli. In the present study, we investigated the population structure and virulence traits of clinical AMC-resistant E. colistrains expressing OXA-1 or IRT and compared these traits to those in a control group of clinical AMC-susceptible E. coliisolates. All OXA-1-producing (n= 67) and IRT-producing (n= 45) isolates were matched by geographical and temporal origin to the AMC-susceptible control set (n= 56). We performed multilocus sequence typing and phylogenetic group characterization for each isolate and then studied the isolates for the presence of 49 virulence factors (VFs) by PCR and sequencing. The most prevalent clone detected was distinct for each group: group C isolates of sequence type (ST) 88 (C/ST88) were the most common in OXA-1 producers, B2/ST131 isolates were the most common in IRT producers, and B2/ST73 isolates were the most common in AMC-susceptible isolates. The median numbers of isolates per ST were 3.72 in OXA-1 producers, 2.04 in IRT producers, and 1.69 in AMC-susceptible isolates; the proportions of STs represented by one unique isolate in each group were 19.4%, 31.1%, and 48.2%, respectively. The sum of all VFs detected, calculated as a virulence score, was significantly higher in AMC-susceptible isolates than OXA-1 and IRT producers (means, 12.5 versus 8.3 and 8.2, respectively). Our findings suggest that IRT- and OXA-1-producing E. coliisolates resistant to AMC have a different and less diverse population structure than AMC-susceptible clinical E. coliisolates. The AMC-susceptible population also contains more VFs than AMC-resistant isolates.

Published
2014
Full Text
View/download PDF

198. Importancia de los controles de calidad para la detección de la resistencia a antibióticos β-lactámicos en enterobacterias

Author

Rivera, Alba, Larrosa, Nieves, Mirelis, Beatriz, and Navarro, Ferran

Abstract

Los β-lactámicos son los antimicrobianos más utilizados para el tratamiento de las infecciones producidas por enterobacterias, y el principal mecanismo de resistencia frente a ellos es la producción de β-lactamasas. Las β-lactamasas de mayor relevancia clínica, debido a su perfil de sustrato y a sus repercusiones epidemiológicas, son las de espectro extendido, las de clase Cy las carbapenemasas. La detección fenotípica de estas β-lactamasas puede ser complicada y se basa, generalmente, en la utilización de inhibidores específicos y en la demostración de la pérdida de actividad frente a β-lactámicos indicadores. A pesar de que diferentes comités internacionales no consideran necesaria la determinación del mecanismo de resistencia ni la interpretación del antibiograma, son varias las voces críticas que estiman imprescindible esta detección y alertan de la conveniencia de interpretar el antibiograma mientras no se disponga de más datos sobre la eficacia clínica de los β-lactámicos en estos casos.

Published
2014
Full Text
View/download PDF

199. Antibiotic Resistance Genes in the Bacteriophage DNA Fraction of Human Fecal Samples

Author

Quirós, Pablo, Colomer-Lluch, Marta, Martínez-Castillo, Alexandre, Miró, Elisenda, Argente, Marc, Jofre, Juan, Navarro, Ferran, and Muniesa, Maite

Abstract

ABSTRACTA group of antibiotic resistance genes (ARGs) (blaTEM, blaCTX-M-1, mecA, armA, qnrA, and qnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs. blaTEM, qnrA, and, blaCTX-M-1were the most abundant, and armA, qnrS, and mecAwere less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra- and extraintestinal environments.

Published
2013
Full Text
View/download PDF

200. Recomendaciones del Comité Español del Antibiograma (COESANT) para el estudio de sensibilidad in vitro a los antimicrobianos por difusión con discos

Author

Rivera, Alba, Viñado, Belén, Benito, Natividad, Docobo, Fernando Manuel, Fernández-Cuenca, Felipe, Fernández-Domínguez, Javier, Guinea, Jesús, López-Navas, Antonio, Moreno, Miguel Ángel, Larrosa, María Nieves, Oliver, Antonio, and Navarro, Ferran

Abstract

Disk diffusion is a well standardized method that provides reliable categorical results to guide antimicrobial therapy in numerous types of infections. Based on the guidelines of the European Committee on Antimicrobial Susceptibility Testing (EUCAST), which are widely implemented in Spain, the Spanish Antibiogram Committee (COESANT) has drawn up recommendations for antimicrobial selection by the disk diffusion technique, including selective reporting and its use for the detection of resistance mechanisms. Factors affecting disk diffusion results, along with advantages and shortcomings of the method, are also discussed.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results