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Introduction. At present, people’s interest in the historical and ethnocultural heritage has increased, and the desire to preserve traditional values for future generations has grown stronger. Song recordings made in the 19th – 20th centuries are evidence of the developed musical and song tradition of the Bashkir people. Due to the collecting efforts of M. A. Burangulov, A. N. Kireev, S. A. Galin, N. D. Shunkarov and others, a whole layer of folk songs has been preserved. During expeditions that have been intensified since the beginning of the 21st century by the Institute of History, Language and Literature of the Ufa Scientific Center of the Russian Academy of Sciences, folklorists are working hard to multifacetedly cataloguize folk knowledge, on the basis of which one can judge the state of traditional modern folklore of the Bashkirs. In our understanding, ‘modern folklore’ is folklore that has existed since the middle of the 20th century to the present, regardless of the environment of existence. Goals. This work aims to consider the genres of traditional musical folklore of the Bashkirs that have survived today, to give a brief description of them, and also to analyze them from the viewpoint of assessing the modern spiritual state of the ethnos. Unlike other genres, musical genres are well preserved in the memory of the population. It is the song and takmaks that are the main genres of modern Bashkir oral and poetic creativity, which makes it possible to reveal the dynamics of the development of folklore. Materials and Methods. The research is based on the author’s expedition materials collected in the 21st century in different regions of the Republic of Bashkortostan and beyond, where the Bashkirs live compactly. They retain collective axiological attitudes and serve as a way of expressing shared emotions. These genres have a high level of demand among the population and therefore quantitatively prevail in expedition records. Folk songs are kept in the memory of people — bearers of folk musical culture, and are not recorded by them in writing. The transmission of musical and folklore works occurs orally. This means that any folk song is perceived and absorbed by each new generation by ear directly at the moment of sounding. Occasionally, songs can be recorded along with their stories and legends. The availability of songwriting histories is a characteristic feature of Bashkir folk songs. Many songs lose their names over time, but they do not completely disappear from the memory of the people, as evidenced by the comments of informants characterizing these works in expressions, such as ‘my mother’s song’, ‘this song was performed by my father’, etc. This phenomenon reflects the strong cultural connection between generations, when performers with special trepidation cherish the memory of their relatives and can reproduce the tune once performed by their father or mother. Along with drawling songs, short four-line songs without a title, drinking songs and takmaks are also common. Takmaks, in turn, are distributed not only orally but also in writing. Modern takmaks are distinguished by great mobility and efficiency, they instantly respond to urgent problems. In the light of recent events, takmaks have appeared on the topic of a pandemic, self-isolation, and online training. Results. A review of folklore materials collected in recent decades shows that the musical genres of Bashkir folklore continue to exist, which means that it is necessary to study not only the current state of the Bashkir song heritage but also its evolution. The folk song, folk singing traditions must be passed on to the younger generation, and only then the folk culture will develop and be preserved for future generations

This text is an introduction to the thematic block of the journal Social Sciences and Humanities Studies on the literary and theatrical aspects of Bosnian drama and theater. It recalls the presence of theatrical and sub-theatrical phenomena on the territory of Bosnia and Herzegovina in antiquity, and then lists the specifics of Bosnian culture in the Middle Ages, from which arises a specific relationship to drama and theater. The attitude of Islamic culture towards theater and drama significantly influenced the status that drama has even after the departure of the Ottoman Empire from Bosnia and Herzegovina. With the arrival of the Austro-Hungarian Empire, there are significant differences concerning European civic theater. Through the most important conclusions from the individual texts that make up this block, the status that Bosnian drama has in our theatrical culture is pointed out.

Croatian National Theatre in Mostar is a relatively new cultural institution when compared to other theatrical institutions. Turbulent incidents of newer Bosnian and Herzegovinian history, as well as historical, political and social changes, left their marks on all levels of the city of Mostar. Therefore, the repertoire of this Theatre, since it has been founded in 1994 (and was primarily named as „War Scene“), needs to be critically analyzed not only in relation to the reality of political and national occurrences of that period but also in comparison with theatrological specificities after the World War II. This paper questions the Theatre’s repertoire under those terms from 1993 to 2005.

Th e works of composers from the Republic of Moldova during the postwar period refl ect a tendency and interest in experimentation with sound and various techniques, in expanding the genre-space and in creatively approaching diff erent musical instruments, which belong to both academic and folk music. Among such instruments is the trumpet. Th e chamber works for this instrument occupy a special place in the trumpet repertoire. We intend to make an analysis of the songs Romance for trumpet and piano by Oleg Negruta and concert Rondo for trumpet and piano by Arcadii Luxemburg, in reference to capitalizing on and using the technical-expressive means of the trumpet, as well as their treatment in relation to the musical dramaturgy and conceptual content.

This article offers an outline of the literary translation flow from Turkish to Arabic between 1923 and 2005 based on a catalogue of translated literary works that were published in this period that was compiled for the purposes of this research. The aim of the article is to understand what kinds of works make it into a literary-linguistic domain when the host system has a turbulent history with the source system (in the case of Turkey and the Arab Middle East, a series of ideological and political factors were behind such turmoil). Based on Even-Zohar’s concept of clusters, the bibliographic data are consulted to trace the systemic infrastructure that helped determine which works from the source literature were admitted into the host literature. The process is carried out through identifying sets of relatable elements (translations, agents) that clustered around six thematic elements to form the repertoire of translated Turkish literature in the Arabic literary system. The analysis shows that the translation flow from Turkish to Arabic continued despite the historical rancour between Turkey and the Arab Middle East.

Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both Zaire Ebolavirus (EBOV) and COVID-19 patient cohorts. We also show unique characteristics absent in Respiratory Syncytial Virus or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risk off-target effects.

We investigate how children in the Sri Lankan Tamil Hindu/Saiva faith community in London learn and use Sanskrit alongside Tamil and/or English and other multimodal and embodied resources to communicate with the Divine.The data were collected as part of a 3-year multi-sited collaborative team ethnography documenting how migrant children become literate in faith settings.The data consist of participant observations across religious education classes, the Temple, and the home, and interviews with the key participant child, Chantia, her brother and the Chief priest at the Temple. The analysis focuses on instances in the data where sacred language learning and performance are thematised. In addition, we analyse a digital video recording of Chantia’s daily morning prayers using transvisuals.Learning Sanskrit consists of integrating a limited set of Sanskrit religious texts and practices, such as key religious concepts, mantras, and poetic verses in children’s evolving religious repertoire and is embedded in children’s everyday religious socialisation across contexts. Chantia unites and syncretises a range of conventionalised semiotic resources, including religious texts in Sanskrit to communicate with the Divine and personalise her act of worship.Children’s religious repertoires are learned, deployed, adapted, and expanded differently depending on the affordances of the socio-cultural context. Chantia’s meaning-making process is much more complex than the rigid categorisation of the different modal resources she deploys, forming an integrated system of communication.Our conceptualisation of Sanskrit sacred language learning is anchored on a multilingual and multimodal perspective that does not privilege Sanskrit over other (sacred) languages nor linguistic over non-linguistic resources.Our paper extends current critique of logocentric perspectives in applied and sociolinguistics to the examination of religious repertoires that are often driven by a communication hierarchy positioning sacred languages at the top and other aspects of communication as secondary. [ABSTRACT FROM AUTHOR]

Pekin ducks are exposed to stressors such as heat stress, enteric pathogens, mycotoxins, and other environmental stressors. We know from wild bird literature that birds communicate through vocalizations. We hypothesized that Pekin ducks would have a diverse repertoire that is affected by the sex, social group, and specific stimuli. We utilized adult Pekin ducks to develop a vocal repertoire. We placed 1 to 4 ducks of varying sexes into a sound chamber with various stimuli used to encourage new vocalizations. Birds were recorded for 20 min with several variations of number and sexes of ducks. Once the ducks were recorded each vocalization that was clipped was named based on a predetermined naming system. We characterized the vocal system of the ducks under each stimulus and social treatment in 4 ways: overall call rates, call diversity, call repertoire, and call spectral properties. In all cases, normality of residuals and homogeneity of variances for GLM and ANOVA models were confirmed using Proc Univariate (SAS v9.4) where a p ≤ 0.05 was considered significant. We found that Pekin ducks produce up to 16 different vocalizations. The treatments had a significant effect on the overall rate of calls given by the ducks (ANOVA: F 6,31 = 8.55, p < 0.0001). Ducks produced the most calls by far when someone was sitting in the chamber with them (30.04 ± 4.45 calls/min). For call diversity, we found that there was a significant main effect of hen number (F 218 = 12.21, p = 0.0004) but no main effect of drake number (F 3,18 = 3.04, p = 0.0555). Cluster analyses indicated that certain types of calls were given under specific conditions. There were generally 6 major clusters of vocal repertoires (R-square = 0.899, Cubic Clustering Criterion = 9.30). Our results suggest that Pekin ducks are affected by the types of stimuli and social environment in how much they vocalize and in the properties of the calls they use. In addition, males and females differ somewhat in the repertoire of the calls they use, and in the spectral properties of their calls. [ABSTRACT FROM AUTHOR]

Humans are constantly exposed to micromycetes, especially filamentous fungi that are ubiquitous in the environment. In the presence of risk factors, mostly related to an alteration of immunity, the non-dermatophyte fungi can then become opportunistic pathogens, causing superficial, deep or disseminated infections. With new molecular tools applied to medical mycology and revisions in taxonomy, the number of fungi described in humans is rising. Some rare species are emerging, and others more frequent are increasing. The aim of this review is to (i) inventory the filamentous fungi found in humans and (ii) provide details on the anatomical sites where they have been identified and the semiology of infections. Among the 239,890 fungi taxa and corresponding synonyms, if any, retrieved from the Mycobank and NCBI Taxonomy databases, we were able to identify 565 moulds in humans. These filamentous fungi were identified in one or more anatomical sites. From a clinical point of view, this review allows us to realize that some uncommon fungi isolated in non-sterile sites may be involved in invasive infections. It may present a first step in the understanding of the pathogenicity of filamentous fungi and the interpretation of the results obtained with the new molecular diagnostic tools.

The purpose of the article is devoted to the study of the tour-enlightening activities of the Ukrainian musical and drama groups in the development the ethnocultural relations in the multicultural conditions of the Russian empires in the late XIX – beginning of the XX centuries. The research methodology of the study consists in the application of historical-cultural, analytical, systematic, and art-scientific methods, which allows you to analyze the artisticenlightening, creative aspects of national theatre groups. The scientific novelty of the work is the analysis of the specific features of the tour-enlightening activities of the Ukrainian musical and drama groups in the context development of ethnocultural relations. Conclusions. During the imperial colonization of culture, the activities of prominent Ukrainian theater artists were aimed at promoting the national culture, and traditions of the Ukrainian nation among a wide range of polyethnic audiences. Dedicated to outstanding masters of the Ukrainian scene, numerous reviews in the columns of periodicals show that viewers and reviewers perceived their work as deeply national and culture-forming. The tourenlightening activity of Ukrainian musical and drama groups was directed the development of ethnocultural relations, contributed to that Ukrainian music and drama theatre became an aesthetic source of national spirit and an important factor in ethnocultural identification. [ABSTRACT FROM AUTHOR]

Animal vocal communication is a broad and multi-disciplinary field of research. Studying various aspects of communication can provide key elements for understanding animal behavior, evolution, and cognition. Given the large amount of acoustic data accumulated from automated recorders, for which manual annotation and analysis is impractical, there is a growing need to develop algorithms and automatic methods for analyzing and identifying animal sounds. In this study we developed an automatic detection and analysis system based on audio signal processing algorithms and deep learning that is capable of processing and analyzing large volumes of data without human bias. We selected the White Spectacled Bulbul (Pycnonotus xanthopygos) as our bird model because it has a complex vocal communication system with a large repertoire which is used by both sexes, year-round. It is a common, widespread passerine in Israel, which is relatively easy to locate and record in a broad range of habitats. Like many passerines, the Bulbul's vocal communication consists of two primary hierarchies of utterances, syllables and words. To extract each of these units' characteristics, the fundamental frequency contour was modeled using a low degree Legendre polynomial, enabling it to capture the different patterns of variation from different vocalizations, so that each pattern could be effectively expressed using very few coefficients. In addition, a mel-spectrogram was computed for each unit, and several features were extracted both in the time-domain (e.g., zero-crossing rate and energy) and frequency-domain (e.g., spectral centroid and spectral flatness). We applied both linear and non-linear dimensionality reduction algorithms on feature vectors and validated the findings that were obtained manually, namely by listening and examining the spectrograms visually. Using these algorithms, we show that the Bulbul has a complex vocabulary of more than 30 words, that there are multiple syllables that are combined in different words, and that a particular syllable can appear in several words. Using our system, researchers will be able to analyze hundreds of hours of audio recordings, to obtain objective evaluation of repertoires, and to identify different vocal units and distinguish between them, thus gaining a broad perspective on bird vocal communication. [ABSTRACT FROM AUTHOR]

The key role of B cells in the pathophysiology of multiple sclerosis (MS) is supported by the presence of oligoclonal bands in the cerebrospinal fluid, by the association of meningeal ectopic B cell follicles with demyelination, axonal loss and reduction of astrocytes, as well as by the high efficacy of B lymphocyte depletion in controlling inflammatory parameters of MS. Here, we use a spontaneous model of experimental autoimmune encephalomyelitis (EAE) to study the clonality of the B cell response targeting myelin oligodendrocyte glycoprotein (MOG). In particular, 94% of SJL/j mice expressing an I-As: MOG92-106 specific transgenic T cell receptor (TCR1640) spontaneously develop a chronic paralytic EAE between the age of 60-500 days. The immune response is triggered by the microbiota in the gut-associated lymphoid tissue, while there is evidence that the maturation of the autoimmune demyelinating response might occur in the cervical lymph nodes owing to local brain drainage. Using MOG-protein-tetramers we tracked the autoantigen-specific B cells and localized their enrichment to the cervical lymph nodes and among the brain immune infiltrate. MOG-specific IgG1 antibodies were detected in the serum of diseased TCR1640 mice and proved pathogenic upon adoptive transfer into disease-prone recipients. The ontogeny of the MOG-specific humoral response preceded disease onset coherent with their contribution to EAE initiation. This humoral response was, however, not sufficient for disease induction as MOG-antibodies could be detected at the age of 69 days in a model with an average age of onset of 197 days. To assess the MOG-specific B cell repertoire we FACS-sorted MOG-tetramer binding cells and clonally expand them in vitro to sequence the paratopes of the IgG heavy chain and kappa light chains. Despite the fragility of clonally expanding MOG-tetramer binding effector B cells, our results indicate the selection of a common CDR-3 clonotype among the Igk light chains derived from both disease-free and diseased TCR1640 mice. Our study demonstrates the pre-clinical mobilization of the MOG-specific B cell response within the brain-draining cervical lymph nodes, and reiterates that MOG antibodies are a poor biomarker of disease onset and progression. [ABSTRACT FROM AUTHOR]

CD8+ T cells play a crucial role in the control and resolution of viral infections and can adopt a wide range of phenotypes and effector functions depending on the inflammatory context and the duration and extent of antigen exposure. Similarly, viral infections can exert diverse selective pressures on populations of clonally related T cells. Technical limitations have nevertheless made it challenging to investigate the relationship between clonal selection and transcriptional phenotypes of virus-specific T cells. We therefore performed single-cell T cell receptor (TCR) repertoire and transcriptome sequencing of virus-specific CD8 T cells in murine models of acute, chronic and latent infection. We observed clear infection-specific populations corresponding to memory, effector, exhausted, and inflationary phenotypes. We further uncovered a mouse-specific and polyclonal T cell response, despite all T cells sharing specificity to a single viral epitope, which was accompanied by stereotypic TCR germline gene usage in all three infection types. Persistent antigen exposure during chronic and latent viral infections resulted in a higher proportion of clonally expanded T cells relative to acute infection. We furthermore observed a relationship between transcriptional heterogeneity and clonal expansion for all three infections, with highly expanded clones having distinct transcriptional phenotypes relative to less expanded clones. Together our work relates clonal selection to gene expression in the context of viral infection and further provides a dataset and accompanying software for the immunological community. [ABSTRACT FROM AUTHOR]

This article refers to the performing art and artistic activities of the vocalist soloists of the famous orchestra of popular music «Fluieraș» led by the renowned musician Sergei Lunchevici, People’s Artist of the USSR. Today, the National Philharmonic of the Republic of Moldova bears his name. The article is focused on four singers — Tamara Ciobanu, Gheorghe Esanu, Nicolae Sulac and Zinaida Julea — that brought an incontestable contribution to the spread of national musical culture and Bessarabian folklore on a worldwide scale.

Introduction. Bashkir wedding is one of the most striking forms of traditional culture. Over centuries of evolution, a complex set of ideological and functional related ritual songs, actions, myths and rituals has formed as an integral festive and theatrical performance. This surprisingly unique performance (drama) reflects the worldview of the people, their poetic and musical culture, including peculiarities of social, everyday and social development. The ideology of caring for the welfare of the family and happy family future has been developed and vividly captured in the wedding rituals. Genotypic plans for the continuation of life, creating a sustainable family in order to ensure continuity and fertility of the new family member ― sister–in–law ― is a major determining code of the Bashkir wedding manifested in the complicated mytho-ritual complex tui (wedding, feast). In it, a large role is given to verbal and song contests (Bash. әйтеш), lamentations (сеңләү), good wishes (теләк, алғыш), ‘yar-yar’ chants, magnificence and farewell songs (takmaks) which are organically woven into the course of the wedding to create a festive atmosphere, give special importance to everything that happens. These work of art have various and rather complex functions, since they not only accompany various moments of the wedding celebration, but also reflect ancient mythological ideas, belief in the power of words and actions. The verbal, song and action creative complex acts as a single syncretic whole with what is happening. Goals. The article examines the role of verbal and poetic works in various parts of the wedding ceremony. They are distinguished by a variety of texts and tunes dedicated to various moments of the wedding and ritual actions addressed to the bride and groom (mostly). Results. The ritual of good wishes goes back to the magic of a kind word, which in general in all types of rituals involves the structuring of events in the name of blessings and the consolidation of a happy future. The article traces the reflection of the emotional state of the bride in various poetic texts, which determines the importance of the moment of the girl’s transition to a qualitatively different state of marriage; the importance of the special semantic load of the songs of the Bashkir wedding for the future family life is noted. In all cultures, edification, parting words generalize the prescriptions and rules of behavior for young people. There are two main places of well-wishes in the wedding drama: in front of the wires from the father’s house (girdling) and in the groom’s house (opening the face). Good wishes and edifications, pronounced in the rites of transitional meaning, represent a kind of collection of etiquette, everyday life and traditions of the people. This is the poeticized wisdom and life experience of people who want the well-being of a girl who goes into a ‘foreign’ environment. In a Bashkir wedding, songs of a greatness, entertaining or humorous nature are performed, which are organically included in the drama of the wedding action and become an integral part of the ceremony as a whole.

CD8+ T cells play a crucial role in the control and resolution of viral infections and can adopt a wide range of phenotypes and effector functions depending on the inflammatory context and the duration and extent of antigen exposure. Similarly, viral infections can exert diverse selective pressures on populations of clonally related T cells. Technical limitations have nevertheless made it challenging to investigate the relationship between clonal selection and transcriptional phenotypes of virus-specific T cells. We therefore performed single-cell T cell receptor (TCR) repertoire and transcriptome sequencing of virus-specific CD8 T cells in murine models of acute, chronic and latent infection. We observed clear infection-specific populations corresponding to memory, effector, exhausted, and inflationary phenotypes. We further uncovered a mouse-specific and polyclonal T cell response, despite all T cells sharing specificity to a single viral epitope, which was accompanied by stereotypic TCR germline gene usage in all three infection types. Persistent antigen exposure during chronic and latent viral infections resulted in a higher proportion of clonally expanded T cells relative to acute infection. We furthermore observed a relationship between transcriptional heterogeneity and clonal expansion for all three infections, with highly expanded clones having distinct transcriptional phenotypes relative to less expanded clones. Together our work relates clonal selection to gene expression in the context of viral infection and further provides a dataset and accompanying software for the immunological community.

The key role of B cells in the pathophysiology of multiple sclerosis (MS) is supported by the presence of oligoclonal bands in the cerebrospinal fluid, by the association of meningeal ectopic B cell follicles with demyelination, axonal loss and reduction of astrocytes, as well as by the high efficacy of B lymphocyte depletion in controlling inflammatory parameters of MS. Here, we use a spontaneous model of experimental autoimmune encephalomyelitis (EAE) to study the clonality of the B cell response targeting myelin oligodendrocyte glycoprotein (MOG). In particular, 94% of SJL/j mice expressing an I-As: MOG92-106 specific transgenic T cell receptor (TCR1640) spontaneously develop a chronic paralytic EAE between the age of 60-500 days. The immune response is triggered by the microbiota in the gut-associated lymphoid tissue, while there is evidence that the maturation of the autoimmune demyelinating response might occur in the cervical lymph nodes owing to local brain drainage. Using MOG-protein-tetramers we tracked the autoantigen-specific B cells and localized their enrichment to the cervical lymph nodes and among the brain immune infiltrate. MOG-specific IgG1 antibodies were detected in the serum of diseased TCR1640 mice and proved pathogenic upon adoptive transfer into disease-prone recipients. The ontogeny of the MOG-specific humoral response preceded disease onset coherent with their contribution to EAE initiation. This humoral response was, however, not sufficient for disease induction as MOG-antibodies could be detected at the age of 69 days in a model with an average age of onset of 197 days. To assess the MOG-specific B cell repertoire we FACS-sorted MOG-tetramer binding cells and clonally expand them in vitro to sequence the paratopes of the IgG heavy chain and kappa light chains. Despite the fragility of clonally expanding MOG-tetramer binding effector B cells, our results indicate the selection of a common CDR-3 clonotype among the Igk light chains derived from both disease-free and diseased TCR1640 mice. Our study demonstrates the pre-clinical mobilization of the MOG-specific B cell response within the brain-draining cervical lymph nodes, and reiterates that MOG antibodies are a poor biomarker of disease onset and progression.

Animal vocal communication is a broad and multi-disciplinary field of research. Studying various aspects of communication can provide key elements for understanding animal behavior, evolution, and cognition. Given the large amount of acoustic data accumulated from automated recorders, for which manual annotation and analysis is impractical, there is a growing need to develop algorithms and automatic methods for analyzing and identifying animal sounds. In this study we developed an automatic detection and analysis system based on audio signal processing algorithms and deep learning that is capable of processing and analyzing large volumes of data without human bias. We selected the White Spectacled Bulbul (Pycnonotus xanthopygos) as our bird model because it has a complex vocal communication system with a large repertoire which is used by both sexes, year-round. It is a common, widespread passerine in Israel, which is relatively easy to locate and record in a broad range of habitats. Like many passerines, the Bulbul’s vocal communication consists of two primary hierarchies of utterances, syllables and words. To extract each of these units’ characteristics, the fundamental frequency contour was modeled using a low degree Legendre polynomial, enabling it to capture the different patterns of variation from different vocalizations, so that each pattern could be effectively expressed using very few coefficients. In addition, a mel-spectrogram was computed for each unit, and several features were extracted both in the time-domain (e.g., zero-crossing rate and energy) and frequency-domain (e.g., spectral centroid and spectral flatness). We applied both linear and non-linear dimensionality reduction algorithms on feature vectors and validated the findings that were obtained manually, namely by listening and examining the spectrograms visually. Using these algorithms, we show that the Bulbul has a complex vocabulary of more than 30 words, that there are multiple syllables that are combined in different words, and that a particular syllable can appear in several words. Using our system, researchers will be able to analyze hundreds of hours of audio recordings, to obtain objective evaluation of repertoires, and to identify different vocal units and distinguish between them, thus gaining a broad perspective on bird vocal communication.

Antibody class switching is a feature of the adaptive immune system which enables diversification of the effector properties of antibodies. Even though class switching is essential for mounting a protective response to pathogens, the in vivo patterns and lineage characteristics of antibody class switching have remained uncharacterized in living humans. Here we comprehensively measured the landscape of antibody class switching in human adult twins using antibody repertoire sequencing. The map identifies how antibodies of every class are created and delineates a two-tiered hierarchy of class switch pathways. Using somatic hypermutations as a molecular clock, we discovered that closely related B cells often switch to the same class, but lose coherence as somatic mutations accumulate. Such correlations between closely related cells exist when purified B cells class switch in vitro, suggesting that class switch recombination is directed toward specific isotypes by a cell-autonomous imprinted state.

• Long-term protection following nasal vaccination in teleosts has not previously been evaluated. • Intranasal (I.N.) vaccination was shown to be safe to at least 12 mo post-vaccination in rainbow trout" • I.P. but not I.N. delivery of ERM bacterin induced significant protection 6 mo post-vaccination. • I.N.-vaccinated fish with live attenuated IHNV vaccine showed the lowest mortality 6 mo post-vaccination. • Spleen repertoire analyses confirmed unique expansions of VH-JH rearrangements depending on the vaccine and delivery route. Previous research demonstrated that bacterial and viral vaccines delivered via the nasal route in rainbow trout (Oncorhynchus mykiss) at 7 and 28 days post-vaccination are highly protective (>95% protection). Long-term protection following nasal vaccination in teleosts has not been evaluated. The goal of this study was to assess efficacy and immune responses at 6 months (mo) post-vaccination (mpv), and long-lasting immune responses at 12 mpv of two different vaccines: an inactivated enteric red mouth disease (ERM) Yersinia ruckeri bacterin and a live attenuated infectious hematopoietic necrosis virus (IHNV) vaccine. Juvenile rainbow trout were vaccinated for Y. ruckeri via intraperitoneal (I.P.) and intranasal (I.N.) routes, and for IHNV by intramuscular (I.M.) and I.N. routes, then challenged at 6 mpv. Immune responses were determined at 6 and 12 mpv. ERM vaccine I.P. delivery elicited significantly higher serum IgM-specific titers that remained elevated compared to mock-vaccinated fish at 6 mpv. By 12 mpv, antibody titers to Y. ruckeri were not significantly different across all treatments. Following Y. ruckeri challenge at 6 mpv, a significant difference in cumulative percent mortality (CPM) was found for I.P.-vaccinated fish but not I.N.-vaccinated fish. I.M. and I.N. vaccination with live attenuated IHNV did not result in significant specific serum IgM titers at 6 or 12 mpv. Yet, I.N.-vaccinated fish showed the lowest CPM 6 mpv indicating long-term protection that does not correlate with systemic IgM responses. Repertoire analyses confirmed unique expansions of VH-JH rearrangements in the spleen of rainbow trout 12 mpv that varied with the type of vaccine and route of vaccination. Combined, these data demonstrate that I.N. vaccination with a live attenuated viral vaccine confers long lasting protection, but I.N. ERM vaccination does not and booster before 6 mpv is recommended. [ABSTRACT FROM AUTHOR]

This research article aims at bringing to the attention of the public and young performers a repertoire less played in our country and aims in addition to broadening and deepening a lesser known musical material and the analysis of new musical forms and genres, the issue being so related to instrumental technique, as well as understanding the elements of specific language. The deepening of these composers and their compositional style helps to diversify the romantic repertoire and to expand the knowledge on the lesser known schools. The peculiarity of these composers consists in the fact that they composed representative works especially for wind instruments. [ABSTRACT FROM AUTHOR]

This article aims to discuss musical preference and children's repertoire in early childhood based on a research with 33 children, aged between 8 months and 4 years old, from the program Music Education for Babies and Children of the Federal University of Bahia, Brazil (2019). In this article we will, initially, present a literature review on the development of musical preference in the early childhood, focusing on how children process and interact with sounds and music. Data were collected through literature review, field diary, questionnaires and video recording. The music classes were held once a week with 50 minutes of duration. During this period, 138 performances were cataloged. The discussion and analysis will focus on the musical and cultural characteristics of the songs performed during the music class, trying to identify how it connects with the children's social and cultural context. The results indicated that the children live in a rich musical environment at home and at school. Although the songs experienced in both contexts come from different genres and styles, they chose traditional children's song for their performances. These songs have strong influences from the main ethnic groups that formed Brazilian culture - Indigenous, Portuguese, and African. From the Portuguese influence we inherited a repertoire formed by simple melodic lines, short melodic intervals, predominance of the major key, anacrusis, and themes about social interactions and daily activities. The indigenous songs bring themes related to the forest, animals, flora, among others. African songs address themes about life, nature and religion. It is common to find words in local dialects such Yoruba and Swahili, and the rhythm is highlighted with a wide variety of percussion instruments. In the conclusion, we discuss how music teachers can contribute to children's musical development, expansion of their repertoire and connecting with their culture. [ABSTRACT FROM AUTHOR]

This article discusses my (first author’s) music therapy placement and research experiences as a postgraduate music therapy student, together with reflections from my current practice as a Registered Music Therapist in private practice. I outline my placement and research approach, and present two main themes from an action research study of my own developing practice with two singing groups for people living with neurological conditions, supported by carers and volunteers. The research question for the study explored what were the goals for individuals and groups, and how did I support the goals through my practice? I used thematic analysis to craft themes in response to the questions. The first theme, named as Gearing for Positivity, was concerned with my efforts to support the groups to feelings of positivity within the sessions through meeting their individual or group goals. The second illustrated theme was Managing my Skills and Vulnerabilities during my placement with the groups. Three further themes were Maintaining the Feel-Good Factor, Copyright, and Safety First. I offer reflections from my current perspective as a Registered Music Therapist. Finally, I offer some recommendations to myself, as a practitioner, and to other novice researchers and future music therapy students. [ABSTRACT FROM AUTHOR]

The purpose of this study was to examine participating orchestras and performed repertoire at the American String Teachers Association's (ASTA) National Orchestra Festival from 2004 through 2020. Participating orchestras (N = 309) primarily consisted of string orchestras and high school–level orchestras with a majority originating from the Southern region of the United States. The most performed full orchestra pieces included Symphony No. 9, op. 95 by Dvořák and Symphony No. 2 by Tchaikovsky, and the most performed string orchestra piece was the Holberg Suite, op. 40 by Grieg. The most frequently cited composers were Dvořák for full orchestra and Mozart for string orchestra. The most frequently cited arrangers were Isaac for full orchestra and Dackow for string orchestra. Female composers and arrangers were highly underrepresented compared with males. Performed repertoire primarily consisted of pieces that conformed to European music traditions. Directors who are unfamiliar with orchestra literature could use these findings as a resource for commonly selected repertoire suitable for high-achieving school orchestras. In addition, findings could provide a catalyst for directors to select music that is more representative of their student population. [ABSTRACT FROM AUTHOR]

Humpback whales (Megaptera novaeangliae) are a highly vocal baleen whale species with a diverse acoustic repertoire. "Song" has been well studied, while discrete "calls" have been described in a limited number of regions. We aimed to quantitatively describe calls from coastal Newfoundland, Canada, where foraging humpback whales aggregate during the summer. Recordings were made in July–August 2015 and 2016. Extracted calls were assigned to call types using aural/visual (AV) characteristics, and then agreement between quantitative acoustic parameters and qualitative call assignments was assessed using a supervised random forest (RF) analysis. The RF classified calls well (96% agreement) into three broad classes (high frequency (HF), low frequency (LF), pulsed (P)), but agreement for call types within classes was lower (LF: 63%; P: 85%; HF: 81%). We found support for a repertoire of 13 call types based on either high (≥70%) RF agreement (9 call types) or high (≥70%) AV agreement between two observers (4 call types). Five call types (swops, droplets, teepees, growls and whups) were qualitatively similar to call types from other regions. We propose that the variable classification agreement is reflective of the graded nature of humpback whale calls and present a gradation model to demonstrate the suggested continuum. [ABSTRACT FROM AUTHOR]

The article deals with the peculiarities of the wind band repertoire and its problems often raised by band conductors, band music researchers and other representatives of the band community in different countries. The main problematic items that have been topical for the wind band repertoire are discerned and commented. Some of these issues are still topical even for the countries with advanced wind band culture; others are more important for the countries in earlier stages of the wind band culture development (presumably, the Baltic States are among them). A serious attitude towards the repertoire selection is essential for all band categories: professional, amateur, and especially for the bands at educational institutions. The students in music schools, comprehensive schools and universities are the future of the wind bandsí personnel and music lovers who will make up the audience of the wind band performances in the future. [ABSTRACT FROM AUTHOR]

The article studies the implied reader of Vladimir Poleganov’s “The Other Dream”. The novel models a reader capable of perceiving nonlinear narrative and palimpsest worlds. The other world is built up as a product of reader reconstruction and thus the novel stages the act of reading as an oneiric experience. [ABSTRACT FROM AUTHOR]

Copyright of Yegah Musicology Journal / Yegah Müzikoloji Dergisi is the property of International Yegah Music Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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The Covid19 pandemic did not cause a dramatic change in the dominant protest repertoire in the United States. Street protests and all the associated behaviors such as marching, carrying signs, chanting, and speeches continued to be the major form of activity. Nonetheless, based on media reports and Crowd Counting Consortium data, we observed three important changes. First, protestors' issues of concern shifted to public health and economic policies. Second, some but not all protestors made tactical adjustments by embracing social distancing or shifting to formats that did not require social distancing such as car caravans. Third, medical facilities much more commonly served as a location for demonstrations. Preliminary analysis also leads us to believe that protestors did not use online tools in dramatically new ways but probably did make use of these tools far more frequently. In short, this was probably more of a quantitative growth than a qualitative shift. [ABSTRACT FROM AUTHOR]

The article analyzes the nutritional and group behaviour of 6 male Bulgarian Murrah buffalo-calves, up to six months old, in the Experimental Farm of the Agricultural Institute - Shumen in the summer of 2021. For three days, visual observations and timing were made. The following more important behavioural reactions were observed: moving, standing (resting), lying down, lying down and ruminating, watering, defecation, urination, comfortable movements, aggressive acts. The interconnection between technology and its elements and the buffalo-calf is complex. The research repertoire of group behaviour of male, growing up buffaloes is inherent in the species. Sporadic differences were observed in the studied groups of animals. Regarding body temperature, pulse and respiratory movements, they were found to be within the physiological norm. [ABSTRACT FROM AUTHOR]

This article introduces the concept of the polymedia repertoire to explore how social meaning is indexed through the interplay of communicative resources at different levels of expression (from choice of media to individual signs) in digitally mediated interactions. The multi-layered polymedia repertoire highlights how people move fluidly between media platforms, semiotic modes and linguistic resources in the course of their everyday interactions, and enables us to locate digital communications within individuals' wider practices. The potential of our theoretical contribution is illustrated through analysis of mobile phone messaging between participants in a large multi-sited ethnography of the communicative practices of multilingual migrants working in linguistically diverse UK city neighbourhoods. Our analysis of mobile messaging exchanges in a day-in-the-life of these networked individuals reveals the importance of device attention in shaping interpersonal interactions, as well as the complex ways in which choices at different levels of a polymedia repertoire are structured by social relationships, communicative purpose and (dis)identification processes. [ABSTRACT FROM AUTHOR]

Abstract T cells, which are involved in adaptive immunity, are essential in the elimination of tumor cells. Mature T cells can specifically recognize the antigen on the major histocompatibility complex (MHC) molecule through T‐cell receptors (TCR). The unique rearrangement mechanisms during T‐cell maturation provide great diversity to TCR, ensuring specific recognition between T cells and antigens. Thus, TCR repertoire analysis occupied an important position in T‐cell regarding research. Nowadays, next‐generation sequencing technology allows the simultaneous detection of TCR sequences with high throughput, and several evaluation indexes facilitate the measure of TCR repertoire. Based on this new methodology, discoveries are made across a range of tumor types. Results have shed light on the TCR repertoire differences between cancer patients and healthy control as well as between individual's lesions, paracancer, and peripheral blood samples. The potential of TCR repertoire as a biomarker for immunotherapy efficacy is also widely studied as TCR repertoire represents different baseline within individuals and shows dynamic change during treatment. Accurate delineation of the T‐cell repertoire can further the understanding of the immune system response to tumorigenesis. Still, existing researches are insufficient to clarify the specific clinical implications of TCR dynamic change and the definite role of TCR repertoire diversity during the treatment process. The results of some studies are even contrary. In this article, we reviewed TCR rearrangement mechanisms and analysis methods. Recent progress of TCR sequencing technology in tumor research is also discussed. In conclusion, intensive studies over an extended range of cancer types and a broadened group of subjects should be carried to solidify the TCR repertoire's position as an immunotherapy biomarker.

Background and Aim The T‐cell receptor (TCR) repertoire was assessed in response to various antigens and was considered to be associated with the pathogenesis of inflammatory bowel disease (IBD). Thus, we performed TCR repertoire analysis to examine the pathology of IBD from changes in the TCR repertoire of memory T cells in the intestinal lamina propria mononuclear cells (LPMCs) and peripheral blood mononuclear cells (PBMCs) of patients with IBD. Methods LPMCs in the surgical specimens and PBMCs were isolated from 12 patients with IBD (5 patients with ulcerative colitis [UC] and 7 patients with Crohn's disease [CD]). PBMCs were collected from 10 healthy individuals as controls. Comprehensive TCR sequence analyses of adaptor‐ligation polymerase chain reaction (PCR) products were performed using MiSeq. Results The diversity of TCR‐α and TCR‐β in PBMCs was significantly lower in patients with IBD than that in controls (P = 0.00084 and 0.0013, respectively). Comparisons of TCR diversity in LPMCs and PBMCs between CD and UC showed that the diversity in LPMC was not affected by diseases, whereas that in PBMCs was significantly lower in CD than in UC (P = 0.045 and 0.049, respectively). Some TCR clones may have shown a specific increase or decrease in CD and UC, and many clones were common to both LPMCs and PBMCs in the same patients. Conclusion The diversity of TCR clones in LPMCs and PBMCs in patients with IBD was significantly lower than that of PBMCs in controls. TCR diversity in PBMCs was particularly low in patients with CD.

ABSTRACTThe Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH repertoire 14 and 64 days after symptom onset showed dramatic dominant clonal expansion but low clonal diversity. IgH repertoire 6 months after disease-free status had few dominant clones but increased diversity. E-binding antibodies appeared abundantly in the repertoire during the early stages of infection but quickly declined after clearance of the virus. Certain VH genes such as VH5-10-1 and VH4-39 appeared to be preferentially enlisted for a rapid antibody response to ZIKV infection. Most of these antibodies require relatively few somatic hypermutations to acquire the ability to bind to the E protein, pointing to a possible mechanism for rapid defence against ZIKV infection. This study provides a unique and holistic view of the dynamic changes and characteristics of the antibody response to ZIKV infection.

The hepatitis B (HB) vaccine effectively prevents the incidence of hepatitis B virus (HBV) infection. However, vaccine failure occurs in 5–10% of the recipients. The precise mechanisms leading to responsiveness to the HB vaccine are poorly understood. High-throughput sequencing (HTS) may help clarify the immune response to the HB vaccine, so we applied this method to investigate whether the HB vaccine induced a specific change in the T-cell receptor (TCR) and B-cell receptor (BCR) repertoires. We conducted HTS of the TCR β chain and BCR IgG heavy (H) chain complementary determining region 3 (CDR3) repertoires in five volunteers before and after the second and third immunizations with the HB vaccine. The HB surface antibody (HBsAb) levels were >10 mIU/ml after the third vaccination in all five participants. The TCR β chain CDR3 repertoire diversity significantly increased, while the BCR IgG H chain CDR3 repertoire diversity significantly decreased after the second vaccination. Although there was no marked inter-individual variation in terms of the numbers of unique reads, it is possible that the TCR β chain and BCR IgG H chain CDR3 repertoires may have changed within the same numbers of unique reads. Our data failed to identify the specific dominant clones that responded to the HB vaccine. In summary, the TCR β chain CDR3 repertoire diversity significantly increased, while the BCR IgG H chain CDR3 repertoire diversity significantly decreased, after the second HB vaccination. These diversity changes might be associated with a better response to the HB vaccine.

Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

In jawed vertebrates, two major T cell populations have been characterized. They are defined as α/β or γ/δ T cells, based on the expressed T cell receptor. Salmonids (family Salmonidae) include two key teleost species for aquaculture, rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) which constitute important models for fish immunology and important targets for vaccine development. The growing interest to decipher the dynamics of adaptive immune responses against pathogens or vaccines has resulted in recent efforts to sequence the immunoglobulin (IG) or antibodies and T cell receptor (TR) repertoire in these species. In this context, establishing a comprehensive and coherent locus annotation is the fundamental basis for the analysis of high-throughput repertoire sequencing data. We therefore decided to revisit the description and annotation of TRA/TRD locus in Atlantic salmon and two strains of rainbow trout (Swanson and Arlee) using the now available high-quality genome assemblies. Phylogenetic analysis of functional TRA/TRD V genes from these three genomes led to the definition of 25 subgroups shared by both species, some with particular feature. A total of 128 TRAJ genes were identified in Salmo , the majority with a close counterpart in Oncorhynchus. Analysis of expressed TRA repertoire indicates that most TRAV gene subgroups are expressed at mucosal and systemic level. The present work on TRA/TRD locus annotation along with the analysis of TRA repertoire sequencing data show the feasibility and advantages of a common salmonid TRA/TRD nomenclature that allows an accurate annotation and analysis of high-throughput sequencing results, across salmonid T cell subsets. [ABSTRACT FROM AUTHOR]

Background: Despite increasing interest in γδ T cells and their non-classical behaviour, most studies focus on animals with low numbers of circulating γδ T cells, such as mice and humans. Arguably, γδ T cell functions might be more prominent in chickens where these cells form a higher proportion of the circulatory T cell compartment. The TCR repertoire defines different subsets of γδ T cells, and such analysis is facilitated by well-annotated TCR loci. γδ T cells are considered at the cusp of innate and adaptive immunity but most functions have been identified in γδ low species. A deeper understanding of TCR repertoire biology in γδ high and γδ low animals is critical for defining the evolution of the function of γδ T cells. Repertoire dynamics will reveal populations that can be classified as innate-like or adaptive-like as well as those that straddle this definition. Results: Here, a recent discrepancy in the structure of the chicken TCR gamma locus is resolved, demonstrating that tandem duplication events have shaped the evolution of this locus. Importantly, repertoire sequencing revealed large differences in the usage of individual TRGV genes, a pattern conserved across multiple tissues, including thymus, spleen and the gut. A single TRGV gene, TRGV3.3, with a highly diverse private CDR3 repertoire dominated every tissue in all birds. TRGV usage patterns were partly explained by the TRGV-associated recombination signal sequences. Public CDR3 clonotypes represented varying proportions of the repertoire of TCRs utilising different TRGVs, with one TRGV dominated by super-public clones present in all birds. Conclusions: The application of repertoire analysis enabled functional annotation of the TCRG locus in a species with a high circulating γδ phenotype. This revealed variable usage of TCRGV genes across multiple tissues, a pattern quite different to that found in γδ low species (human and mouse). Defining the repertoire biology of avian γδ T cells will be key to understanding the evolution and functional diversity of these enigmatic lymphocytes in an animal that is numerically more reliant on them. Practically, this will reveal novel ways in which these cells can be exploited to improve health in medical and veterinary contexts. [ABSTRACT FROM AUTHOR]

The field of family language policy has flourished in recent years addressing an ever-increasing breadth of transnational populations, family types, and linguistic configurations. Furthermore, diverse established and innovative methodological approaches have unlatched the door to enhanced understandings of family multilingualism. A focus on ethnographic approaches is in line with current trends in sociolinguistic enquiry, providing deep insight into multilingual families, as demonstrated by this thematic issue on 'Exploring the multilingual family repertoire: Ethnographic approaches', edited by Luk Van Mensel and Maartje De Meulder. An underlying current in the studies of this special issue is the notion of the family as a space, negotiated through the multilingual family repertoire – multilingual practices that embrace the speakers' lived experiences. This concluding discussion analyzes the ways in which the four articles address the family as a multilingual space and highlights some of the more salient aspects that deserve further attention, looking beyond at new potential approaches to critically addressing family multilingualism. [ABSTRACT FROM AUTHOR]

This essay offers an overview of language and gender research as it unfolded in a particular 'Place': Japan. In the past thirty years, Japanese language and gender/sexuality relations have been characterised both domestically and globally as special, sometimes as unique, due to the existence of distinct joseigo 'women's language' and danseigo 'men's language'. A preferential focus on the surface-segmentable forms (pronouns, sentence final particles, etc.) over discursive features and a limited focus on Standard Japanese in the early years of Japanese language and gender research has led to a tendency to view 'the' Japanese language as a homogeneous unity and to the reification of the three critical categories, 'Japan', 'language' and 'gender'. In this essay, I discuss the problematic nature of the three critical terms, and suggest ways in which Japanas-Place might profitably be renarrated as the complex place it is and Japanese language, gender and sexuality relations revisited as they operate within that complexity. [ABSTRACT FROM AUTHOR]

Using conversation analytic methods and concepts, this article examines a language tutorial among Thai and Japanese college students, focusing on the use of multilingual repertoires for organizing this activity. The study responds to a call for dialogic studies of multilingual interactions. A 79‐minute video‐recorded session was analyzed for the coordinated use of multilingual and multimodal resources as well as the role of sequential routines in the cooperative pursuit of relevant interactional projects. Findings demonstrate the contingent and variable nature of cooperative behaviors that are shaped not simply by a priori goals, but emergent interactional projects. They also exemplify reciprocal language learning wherein the participants alternate between expert and novice roles with respect to Thai and Japanese. The article concludes with the implications of the study for language learning and teaching in the context of globalization. [ABSTRACT FROM AUTHOR]

According to current theory, the development of different conspicuous ornaments may provide multiple, redundant or unreliable information. However, multiple visual and acoustic traits have not often been tested at the same time. In this study, we analyse whether black tie size, yellow breast colour indexes (chroma and hue) and song parameters (repertoire size, strophe length, maximum and minimum frequency) are inter-correlated in male great tits (Parus major). We found that males with larger black ties sang greater repertoires and had higher song frequencies, providing redundant information. However, song parameters did not correlate with hue and chroma of the yellow breast coloration, supporting the idea that they convey different units of information. Results therefore stress that since different plumaged patches can transmit very different units of information, bird song will correlate to plumage coloration traits depending on the patch and the colour considered, an aspect rarely taken into account in previous studies relating song to plumage colour traits. [ABSTRACT FROM AUTHOR]