201. The corneal pocket assay.
- Author
-
Ziche M and Morbidelli L
- Subjects
- Adenoviridae genetics, Animals, Female, Genetic Vectors genetics, Immunohistochemistry, Male, Mice, Rabbits, Rats, Species Specificity, Transduction, Genetic, Cornea blood supply, Cornea drug effects, Corneal Neovascularization drug therapy, Corneal Neovascularization genetics, Corneal Neovascularization pathology, Corneal Neovascularization surgery, Disease Models, Animal
- Abstract
The cornea in most species is physiologically avascular, and thus this assay allows the measurement of newly formed vessels. The continuous monitoring of neovascular growth in the same animal allows the evaluation of drugs acting as suppressors or stimulators of angiogenesis. Under anesthesia a micropocket is produced in the cornea thickness and the angiogenesis stimulus (tumor tissue, cell suspension, growth factor) is placed into the pocket in order to induce vascular outgrowth from the limbal capillaries. Neovascular development and progression can be modified by the presence of locally released or applied inhibitory factors or by systemic treatments. In this chapter the experimental details of the avascular cornea assay, the technical challenges, and advantages and disadvantages in different species are discussed. Protocols for local drug treatment and tissue sampling for histology and pharmacokinetic profile are reported.
- Published
- 2015
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