723 results on '"Pereira, José Alberto"'
Search Results
202. Diabetes Mellitus Tipo 2: Relação da Capacitação, Conhecimentos e Qualidade de Vida com a Ansiedade, o Stress e a Depressão
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Pereira, José Alberto Correia Lopes Longras and Caetano, Inês Rosendo Carvalho e Silva
- Subjects
Depression ,Diabetes Mellitus Tipo 2 ,Depressão ,Ansiedade ,Diabetes Mellitus Type 2 ,Anxiety ,Stress - Abstract
Trabalho Final do Mestrado Integrado em Medicina apresentado à Faculdade de Medicina Background: Diabetes Mellitus (DM) is a chronic disease that affects a great part of the world's population. Depression, anxiety, and stress are growing problems in our society and are associated with poor glycemic control and increased complications in DM. Thus, it is important to study the presence of psychopathology in these patients.Objective: Assess the relationship between psycopathology (depression, anxiety and stress) and the empowerment, quality of life and knowledge about their disease in a group of people diagnosed with type 2 Diabetes Mellitus.Methods: Cross-sectional observational study in a convenience sample of type 2 diabetic patients followed in a primary care unit (USF Pulsar). Data collection performed between August 22, 2017 and October 23, 2017. An instrument that included the Portuguese versions of five different questionnaires - DKT, DES-SF, EQ-5D, DHP-18 and EADS-21 -, a sociodemographic questionnaire and an informed consent was applied through interview. A descriptive and inferential statistical analysis was performed. The Spearman correlation test and the Mann-Whitney U test were applied.Results: Sample of 31 individuals, 54.8% female, with a median age of 70 ± 10.9 years, a median of HbA1C 6.25 ± 0.71% and the time since diagnosis with a median of 10 ± 9, 52 years. There were negative and statistically significant correlations (p
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- 2018
203. Plantas espontâneas em floração como recurso alimentar para adultos de Chrysoperla carnea s.l. no agroecossistema olival
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Villa, María, Somavilla, Iana, Santos, Sónia A.P., López-Sáez, José António, and Pereira, José Alberto
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Seleção de recursos ,Crisopídeos ,Proteção biológica por conservação ,Hábitos alimentares - Abstract
As larvas de Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) são consumidores generalistas de pragas nos agroecossistemas, nomeadamente no olival. Os adultos alimentam-se de meladas de hemípteros, pólen e néctar de plantas desempenhando a flora adventícia do olival um papel relevante em proteção biológica de conservação. Considerando que o pólen pode ser um recurso florístico essencial para a reprodução e fecundidade no predador e que o conhecimento acerca da dieta polínica de C. carnea é escasso, o presente trabalho teve por objetivo estudar a potencial seleção de tipos polínicos consumidos por C. carnea s.l. no agroecossistema olival. De abril a dezembro de 2012 e 2013, em olivais com coberto vegetal na região de Mirandela foram capturados adultos de C. carnea s.l. na copa da oliveira. Em laboratório, procedeu-se à remoção do conteúdo estomacal dos insetos para identificação, ao microscópio ótico, dos diferentes tipos polínicos. Paralelamente realizaram-se inventários das plantas em floração nos olivais e parcelas adjacentes de vegetação herbácea e arbustiva para comparação dos tipos polínicos consumidos e presentes no agroecossistema. Os resultados indicaram que os indivíduos de C. carnea s.l. consumiram pólen de diferentes plantas anemófilas e entomófilas pertencentes aos extratos arbóreo e arbustivo (Fabaceae, Pinaceae, Cistaceae ou Ericaceae) e ao extrato herbáceo (Asteraceae, Apicaceae, Brassicaceae, Poaceae, tipo Rumex o tipo Plantago). Por outro lado, verificou-se ainda que os adultos de C. carnea se alimentaram não só do pólen das flores mas também do outro pólen que provavelmente se encontra depositado sobre a superfície da vegetação, o que poderá representar um reservatório alimentar para períodos de escassez. Os resultados obtidos evidenciam a importância da flora espontânea nos agroecossistemas e podem contribuir para a gestão destas espécies no olival com vista ao fomento da comunidade de inimigos naturais de pragas da cultura. info:eu-repo/semantics/publishedVersion
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- 2018
204. Compostos com potencial anti-inflamatório em azeites monovarietais da região de Trás-os-Montes
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Rodríguez-Alcalá, Luís Miguel, Correia, Inês, Pimentel, Lígia, Pereira, José Alberto, Gomes, Ana Maria, Pintado, Manuela, and Veritati - Repositório Institucional da Universidade Católica Portuguesa
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Oleuropeína ,Dieta mediterrânica ,Compostos bioativos ,Risco cardiovascular - Abstract
Submitted by Maria João Pinto (mjpinto@porto.ucp.pt) on 2018-12-28T17:56:31Z No. of bitstreams: 1 livro_de_resumos_viii_simpósio_nacional_de_olivicultura__junho_2018-70-70.pdf: 505881 bytes, checksum: b6a867bc1da6fe11c0369d59bbe77d2d (MD5) Approved for entry into archive by Maria João Pinto (mjpinto@porto.ucp.pt) on 2018-12-28T17:56:42Z (GMT) No. of bitstreams: 1 livro_de_resumos_viii_simpósio_nacional_de_olivicultura__junho_2018-70-70.pdf: 505881 bytes, checksum: b6a867bc1da6fe11c0369d59bbe77d2d (MD5) Made available in DSpace on 2018-12-28T17:56:42Z (GMT). No. of bitstreams: 1 livro_de_resumos_viii_simpósio_nacional_de_olivicultura__junho_2018-70-70.pdf: 505881 bytes, checksum: b6a867bc1da6fe11c0369d59bbe77d2d (MD5) Previous issue date: 2018 info:eu-repo/semantics/publishedVersion
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- 2018
205. Survival of Psyttalia concolor: Do flowers make the difference?
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Pinheiro, Lara A., Pereira, José Alberto, Medina, Pilar, and Santos, Sónia A.P.
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Bactrocera oleae ,Biological control ,Olive grove ,Non-crop plants ,Parasitoid - Abstract
In sustainable olive groves, the biological control of the olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) should be achieved by maximizing the action of its natural enemies. In this work, we are focused on Psyttalia concolor (Szépligeti) (Hymenoptera: Braconidae), a synovigenic koinobiont larval-pupal endoparasitoid of many Tephritidae that has been released in several Mediterranean and Californian (USA) olive-groves against B. oleae reaching different levels of success. In this context, the diversification of non-crop plants within and around olives groves is an effective strategy to support the population of this parasitoid. Plants provide nutritional supplements (pollen and/or nectar) for maintenance, survival and egg maturation but also shelter and alternative hosts. In this study, ten flowering non-crop plants that naturally occur in olive groves, distributed throughout six different families: Apiaceae (Daucus carota L. and Foeniculum vulgare L.); Asteraceae (Bellis perennis L.; Calendula arvensis L.; Crepis vesicaria L.; Dittrichia viscosa (L.) Greuter); Brassicaceae (Brassica barrelieri (L.) Janka); Geraniaceae (Geranium molle L.) Malvaceae (Malva sylvestris L.) and Plantaginaceae (Veronica persica Poir) were tested in order to identify flowers with capacity to increase survival of P. concolor. Parasitoids exposed to flowers increased their survival to, on average, three to five days, except for M. sylvestris, V. persica and F. vulgare that significantly increased longevity of females and males of P.concolor. Interestingly, for males, all Asteraceae species tested improved their longevity, when compared to females. These findings suggest that species such as M. sylvestris could be a suitable species for establishing in olive groves. info:eu-repo/semantics/publishedVersion
- Published
- 2018
206. Ancient olive trees as a source of olive oils rich in phenolic compounds
- Author
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Rodrigues, Nuno, primary, Casal, Susana, additional, Pinho, Teresa, additional, Peres, António M., additional, Bento, Albino, additional, Baptista, Paula, additional, and Pereira, José Alberto, additional
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- 2019
- Full Text
- View/download PDF
207. EcoPred: an educational individual based model to explain biological control, a case study within an arable land
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Benhadi-Marín, Jacinto, primary, Pereira, José Alberto, additional, Sousa, José Paulo, additional, and Santos, Sónia A.P., additional
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- 2019
- Full Text
- View/download PDF
208. Characterization of wild and centenarian olive trees for their valorization = Caracterización de acebuches y olivos centenarios para su valoración
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Sousa Rodrigues, Nuno Miguel de, Bento, Albino António, Pereira, José Alberto Cardoso, Baptista, Paula, Ingenieria Agroforestal, Sousa Rodrigues, Nuno Miguel de, Sousa Rodrigues, Nuno Miguel de, Bento, Albino António, Pereira, José Alberto Cardoso, Baptista, Paula, Ingenieria Agroforestal, and Sousa Rodrigues, Nuno Miguel de
- Abstract
The species Olea europaea L. is constituted by two varieties, the cultivated one - the olive tree, O. europaea subsp. europaea var. europaea; and the wild form - the wild olive tree or oleaster, O. europaea subsp. europaea var. sylvestris. These varieties were differentiated during the domestication process, and from the cultivated form a high number of cultivars evolved over the years. In the last decades, due to the crop intensification a few number of well adapted or productive cultivars have been used in the new olive plantations, leading to the abandonment of minor cultivars and to a loss of the olive genetic heritage. Nevertheless, in the last years, a niche of more informed and demanding consumers appeared, searching for olive oils that combine their richness in health promoters, like polyphenols, tocopherols and sterols, with a differentiated sensory profile. In this context, the general objective of this work was to characterize genetically and morphologically oleander populations and centenarian olive trees from the northeast of Portugal, as well as, to chemically and sensory evaluate the extracted oils, aiming the selection of plant specimens with the purpose of future valorization. Three oleander populations from Alijó, Moncorvo and Vila Nova de Foz Côa (VNFC) regions and 28 specimens of centenarian olive trees, grown in Mirandela region (Suçães), some of them from known cultivars (cvs. Lentisca, Madural, Rebolã, Redondal, Verdeal and Verdeal Transmontana) were characterized. The comparison of genetic diversity, structure and phylogenetic relationships within and among the oleaster and centenarian plants were studied using microsatellite markers. High genetic diversity was observed in both oleaster and centenarian plants, with no differences between them in the amount of genetic diversity. Population structure analysis suggests genetic differentiation between the studied varieties. The oils of the three oleaster populations were evaluated considering the
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- 2018
209. Effect of parasitoid competition, ant exclusion and carbohydrate sources on biological control fo Saissetia oleae on olive trees = Efecto de la competición de los parasitoides, la exclusión de hormigas y las fuentes de hidratos de carbono en el control biológico de Saissetia oleae en olivos
- Author
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Martins Marrao, Rosalina, Tena Barreda, Alejandro, Pereira, José Alberto Cardoso, Botanica, Martins Marrao, Rosalina, Martins Marrao, Rosalina, Tena Barreda, Alejandro, Pereira, José Alberto Cardoso, Botanica, and Martins Marrao, Rosalina
- Abstract
The black scale, Saissetia oleae (Olivier), is considered a secondary pest of olive tree in Portugal, where it is attacked by a complex of natural enemies that control its population. Parasitoids of genus Metaphycus (Hymenoptera: Encyrtidae) and Coccophagus (Hymenoptera: Aphelinidae) are among the main natural enemies. In this thesis, the several aspects of the successful natural biological control of this scale are analyzed in the field and laboratory. In detail, we analyzed: (i) whether the facultative autoparasitoid Coccophagus lycimnia (Walker) can adversely affect the population densities of Metaphycus spp. parasitoids and disrupt the biological control of S. oleae; (ii) the effect of ant-exclusion on the biological control of S. oleae and its parasitoid complex; and (iii) the effect of the carbohydrate sources available in the field (nectar and honeydew) on the survival of parasitoids of both genus. For the development of the first objective, the population densities of the scale, the primary parasitoids of the genus Metaphycus and the autoparasitoid C. lycimnia were recorded for three consecutive seasons in 28 olive groves. The populations of the parasitoids followed opposite patterns. At the end of the life cycle of the S. oleae (May), the number of Metaphycus spp. observed was practically nil whereas the number of C. lycimnia was five times greater than at the beginning of the assay. In September and November, Metaphycus spp. parasitoids parasitized smaller scales than C. lycimnia, demonstrating that Metaphycus spp. parasitoids are superior at exploitative competition. Metaphycus spp. and C. lycimnia sex ratio was similar in September, but in November the sexual proportion of Metaphycus spp. was higher than that of C. lycimnia. The facultative autoparasitoid C. lycimnia tended to lay male eggs on Metaphycus spp. females. The density of S. oleae decreased from September to May, when the ratio S. oleae-C. lycimnia reached 2:1. Altogether, these results demons
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- 2018
210. Characterization of wild and centenarian olive trees for their valorization = Caracterización de acebuches y olivos centenarios para su valoración
- Author
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Bento, Albino António, Pereira, José Alberto Cardoso, Baptista, Paula, Ingenieria Agroforestal, Sousa Rodrigues, Nuno Miguel de, Bento, Albino António, Pereira, José Alberto Cardoso, Baptista, Paula, Ingenieria Agroforestal, and Sousa Rodrigues, Nuno Miguel de
- Abstract
The species Olea europaea L. is constituted by two varieties, the cultivated one - the olive tree, O. europaea subsp. europaea var. europaea; and the wild form - the wild olive tree or oleaster, O. europaea subsp. europaea var. sylvestris. These varieties were differentiated during the domestication process, and from the cultivated form a high number of cultivars evolved over the years. In the last decades, due to the crop intensification a few number of well adapted or productive cultivars have been used in the new olive plantations, leading to the abandonment of minor cultivars and to a loss of the olive genetic heritage. Nevertheless, in the last years, a niche of more informed and demanding consumers appeared, searching for olive oils that combine their richness in health promoters, like polyphenols, tocopherols and sterols, with a differentiated sensory profile. In this context, the general objective of this work was to characterize genetically and morphologically oleander populations and centenarian olive trees from the northeast of Portugal, as well as, to chemically and sensory evaluate the extracted oils, aiming the selection of plant specimens with the purpose of future valorization. Three oleander populations from Alijó, Moncorvo and Vila Nova de Foz Côa (VNFC) regions and 28 specimens of centenarian olive trees, grown in Mirandela region (Suçães), some of them from known cultivars (cvs. Lentisca, Madural, Rebolã, Redondal, Verdeal and Verdeal Transmontana) were characterized. The comparison of genetic diversity, structure and phylogenetic relationships within and among the oleaster and centenarian plants were studied using microsatellite markers. High genetic diversity was observed in both oleaster and centenarian plants, with no differences between them in the amount of genetic diversity. Population structure analysis suggests genetic differentiation between the studied varieties. The oils of the three oleaster populations were evaluated considering the
- Published
- 2018
211. Effect of parasitoid competition, ant exclusion and carbohydrate sources on biological control fo Saissetia oleae on olive trees = Efecto de la competición de los parasitoides, la exclusión de hormigas y las fuentes de hidratos de carbono en el control biológico de Saissetia oleae en olivos
- Author
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Tena Barreda, Alejandro, Pereira, José Alberto Cardoso, Botanica, Martins Marrao, Rosalina, Tena Barreda, Alejandro, Pereira, José Alberto Cardoso, Botanica, and Martins Marrao, Rosalina
- Abstract
The black scale, Saissetia oleae (Olivier), is considered a secondary pest of olive tree in Portugal, where it is attacked by a complex of natural enemies that control its population. Parasitoids of genus Metaphycus (Hymenoptera: Encyrtidae) and Coccophagus (Hymenoptera: Aphelinidae) are among the main natural enemies. In this thesis, the several aspects of the successful natural biological control of this scale are analyzed in the field and laboratory. In detail, we analyzed: (i) whether the facultative autoparasitoid Coccophagus lycimnia (Walker) can adversely affect the population densities of Metaphycus spp. parasitoids and disrupt the biological control of S. oleae; (ii) the effect of ant-exclusion on the biological control of S. oleae and its parasitoid complex; and (iii) the effect of the carbohydrate sources available in the field (nectar and honeydew) on the survival of parasitoids of both genus. For the development of the first objective, the population densities of the scale, the primary parasitoids of the genus Metaphycus and the autoparasitoid C. lycimnia were recorded for three consecutive seasons in 28 olive groves. The populations of the parasitoids followed opposite patterns. At the end of the life cycle of the S. oleae (May), the number of Metaphycus spp. observed was practically nil whereas the number of C. lycimnia was five times greater than at the beginning of the assay. In September and November, Metaphycus spp. parasitoids parasitized smaller scales than C. lycimnia, demonstrating that Metaphycus spp. parasitoids are superior at exploitative competition. Metaphycus spp. and C. lycimnia sex ratio was similar in September, but in November the sexual proportion of Metaphycus spp. was higher than that of C. lycimnia. The facultative autoparasitoid C. lycimnia tended to lay male eggs on Metaphycus spp. females. The density of S. oleae decreased from September to May, when the ratio S. oleae-C. lycimnia reached 2:1. Altogether, these results demons
- Published
- 2018
212. Use of response surface methodology (Rsm) for the identification of the best extraction conditions for headspace solid-phase micro extraction (hs-spme) of the volatile profile of cv. arbequina extra-virgin olive oil
- Author
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Universidad de Granada, Fundação para a Ciência e a Tecnologia (Portugal), European Commission, Ministério da Educação (Brasil), Borges, Thays H., Ramalhosa, E., Seiquer, Isabel, Pereira, José Alberto, Universidad de Granada, Fundação para a Ciência e a Tecnologia (Portugal), European Commission, Ministério da Educação (Brasil), Borges, Thays H., Ramalhosa, E., Seiquer, Isabel, and Pereira, José Alberto
- Abstract
The effect of the experimental conditions on assessing the global volatile profile of extra virgin olive oils (EVOO) by headspace solid-phase micro-extraction/gas chromatography-mass spectrometry (HS-SPME/GC–MS) is studied to obtain maximization of the total peak areas of the compounds. Response Surface Methodology (RSM) is applied to Arbequina EVOO, and the influence of oil quantity, extraction time, and extraction temperature on the total area and extraction of the major desirable compounds is analyzed. The experimental data are adequately fitted into second-order polynomial models with non-significant lack of fit (p > 0.05) and coefficients of determination (R and R -adjusted) higher than 0.88 and 0.78, respectively. A strong similarity is found between the predicted and experimental values. Furthermore, the surface plots show that the extraction of the volatile compounds is favored with increasing extraction temperature, time, and oil quantity. The extraction conditions to obtain the maximum response of “green” volatile compounds from Arbequina olive oil by HS-SPME placed in 50 mL vials are 4.6 g of oil, 43 min, and 59 C. Practical Applications: The findings of the present work show that specific conditions of HS-SPME/GC–MS affect the extraction of volatile compounds from cv. Arbequina olive oil and establish the optimum extraction parameters to more efficiently determine the global profile of this fraction, taking into account the organoleptic characteristics of the cultivar. The results also contribute to the knowledge of the volatile profile of the monovarietal olive oil cv. Arbequina, which is one of most cultivated and consumed types of olive oil worldwide.
- Published
- 2018
213. Distribution and relative abundance of insect vectors of xylella fastidiosa in olive groves of the iberian peninsula
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European Commission, Morente, Marina, Cornara, Daniele, Plaza, María, Durán, José Manuel, Capiscol, Carmen, Trillo, Raquel, Ruiz, Manuel, Ruz-Ortiz, Carmen, Sanjuan, Susana, Pereira, José Alberto, Moreno, Aránzazu, Fereres, Alberto, European Commission, Morente, Marina, Cornara, Daniele, Plaza, María, Durán, José Manuel, Capiscol, Carmen, Trillo, Raquel, Ruiz, Manuel, Ruz-Ortiz, Carmen, Sanjuan, Susana, Pereira, José Alberto, Moreno, Aránzazu, and Fereres, Alberto
- Abstract
The phytosanitary emergency caused by the spread of Xylella fastidiosa in the Mediterranean has raised demands for a better understanding of the ecology of its presumed and candidate insect vectors. Here, we present the results of a two-year survey carried out in olive groves across southern, eastern and Central Spain and northeastern Portugal. Several sampling methods were tested and compared to select the most appropriate to estimate population levels of potential vectors of X. fastidiosa. The spittlebugs Philaenus spumarius and Neophilaenus campestris (Hemiptera: Aphrophoridae) were the main species associated with olive groves. Both species were widely present on herbaceous ground vegetation within the olive groves; P. spumarius mainly associated with Asteraceae and N. campestris with Poaceae. Due to the patchy distribution of spittlebugs within the olive groves, sweep nets were the most effective and least time-consuming sampling method for the estimation of population size both in the ground cover and tree canopies. Trends in population density showed that spittlebugs can be abundant on ground vegetation but very rare on olive canopies. Spittlebugs disperse in late spring to non-cultivated hosts that act as natural reservoirs. In late fall, adults return to the olive groves for oviposition. However, olive trees may act as transient hosts for spittlebugs and high population densities of these insect vectors should be avoided in areas where X. fastidiosa is present.
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- 2018
214. Do non-crop areas and landscape structure influence dispersal and population densities of male olive moth?
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Villa, María, Santos, Sónia A. P., Pascual, Susana, and Pereira, José Alberto
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POPULATION density ,POLLEN ,MOTHS ,TREE crops ,OLIVE ,AGRICULTURAL pests - Abstract
The permeability of the crop surroundings to pests and the landscape structure can influence pest dispersal between crop patches as well as its abundance within the crop. In this work, we analyzed the dispersal of the olive moth Prays oleae (Bernard) throughout the olive grove surroundings and their abundance within the crop following three approaches: (i) pollen grains settled on bodies of olive moths collected in olive groves were identified and compared with flora occurring on the surrounding patches; (ii) the capability of P. oleae males to penetrate non-crop patches was analyzed (iii) the effect of the landscape structure on the abundance of the three generations of the olive moth was studied. Pollen grains of scrubs and other trees occurring in the crop surroundings, such as Cistus sp., Quercus sp., Juniperus-type or Pinaceae were identified on P. oleae bodies suggesting that P. oleae penetrates into non-crop habitats. Additionally, woody and, to a lesser degree, herbaceous patches, did not constitute barriers for P. oleae. Finally, more complex and heterogeneous patches presented lower numbers of captures of P. oleae. These results give new insights into the movements of the olive moth in the olive grove surroundings and suggest that the management of non-crop areas could influence this pest abundance. [ABSTRACT FROM AUTHOR]
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- 2021
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215. Social media intelligence versus inteligência competitiva - os dados das redes sociais na informação empresarial: o caso da ANA - Aeroportos de Portugal, S.A
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Pereira, José Alberto Sardo Ramos and Terra, Ana Lúcia
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Gestão ,Inteligência competitiva ,Redes sociais virtuais ,Intelligence ,Competitive intelligence ,Web social media ,Social media intelligence ,Socia Media Intelligence - Abstract
Submitted by Iolanda Valente (ivalente@iscap.ipp.pt) on 2018-03-22T09:32:34Z No. of bitstreams: 1 jose_pereira_MIE_2017.pdf: 3793546 bytes, checksum: 5578926fa159994f268f5033bc86b126 (MD5) Made available in DSpace on 2018-03-22T09:32:34Z (GMT). No. of bitstreams: 1 jose_pereira_MIE_2017.pdf: 3793546 bytes, checksum: 5578926fa159994f268f5033bc86b126 (MD5) Previous issue date: 2017-12-18
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- 2017
216. La interacción entre presas afecta a la respuesta funcional de las arañas y a su preferencia por los distintos tipos de presa
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Benhadi-Marín, Jacinto, Pereira, José Alberto, Barreales, David, Sousa, José Paulo, and Santos, Sónia A.P.
- Abstract
info:eu-repo/semantics/publishedVersion
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- 2017
217. Comunidade bacteriana associada à filosfera da oliveira e a sua capacidade antagonista contra Pseudomonas savastanoi pv. savastanoi
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Mina, Diogo, Santos, Alba, Pereira, José Alberto, Lino-Neto, T., Baptista, Paula, and Universidade do Minho
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Ciências Naturais::Ciências Biológicas ,Comunidade bacteriana ,Filosfera ,Tuberculose ,Oliveira ,Oleae europaea - Abstract
A tuberculose da oliveira é uma das doenças mais importantes do olival, pelos prejuízos económicos que pode causar derivada das perdas de produção e de qualidade dos produtos obtidos. É provocada pela bactéria Pseudomonas savastanoi pv. savastanoi (Psv), que causa a formação de nódulos nos caules e ramos, raramente afetando as folhas e os frutos. A inexistência de métodos de luta eficazes contra esta doença associado ao crescimento da agricultura biológica, tem incentivado a realização de estudos que visem a identificação de agentes de luta biológica que possam ser usados contra a tuberculose da oliveira. Este trabalho teve como objetivo isolar bactérias da filosfera da oliveira e efetuar uma seleção dos isolados com maior capacidade de inibir o crescimento de Psv. As bactérias endofiticas e epifiticas foram isoladas de folhas, caules e nódulos das cvs. "Cobrançosa" (tolerante à doença) e "Verdeal Transmontana" suscetível à doença), pela inoculação destes segmentos esterilizados ou por diluições decimais sucessivas de material não esterilizado em meio de cultura Luria-Broth (LB), respetivamente. Os isolados obtidos foram identificados por sequenciação da região 16Sdo rDNA e, a sua atividade antagonista contra Psvfoi avaliada recorrendo ao método da co-cultura em meio LB.Os valores dos raios internos (na região inter-inóculos) das colónias, foram determinados e comparados com o controlo (culturas com um inóculo de Psv). Para os isolados que demonstraram uma evidente ação antagonista, foram ainda avaliados os mecanismos a ela associados em condições in vitro, nomeadamente a produção de enzimas líticas, compostos voláteis e de sideróforos, utilizando métodos qualitativos. A análise de escala multidimensional não métrica (NMDS) associada à análise de similaridade ANOSIM mostrou que a composição da comunidade bacteriana endofitica e epifitica diferiu entre cultivares e órgão vegetal amostrado, apresentando os caules o maior número de espécies exclusivas pertencentes aos géneros Agrococcus e Frondihabitans. Dos 60 isolados testados, cerca de 20% exibiram capacidade para inibir o crescimento de Psv em mais de 50% face ao controlo. De uma maneira geral, as espécies isoladas de caules e nódulos da cv. Cobrançosa apresentaram uma maior capacidade antagonista (mais de 20%) face aos isolados obtidos da cv. Verdeal Transmontana, tanto ao nível da comunidade endofitica como epifitica. A produção de enzimas líticas, em especial de proteases, bem como de sideroforos e de compostos voláteis pelos isolados bacterianos testados, pareceu estar envolvida no mecanismo antagonista contra Psv. Os resultados sugerem que a cultivar e o tipo de órgão vegetal tem influência no processo de colonização das bactérias na oliveira. A menor susceptibilidade da cv. "Cobrançosa" à tuberculose parece estar relacionada com a presença de bactérias endofiticas e epifiticas na filosfera, em especial de antagonistas. O efeito destes microrganismos tem de ser confirmado em ensaios in planta em trabalhos futuros., Os autores agradecem à Fundação para a Ciência e a Tecnologia (FCT,Portugal) e ao FEDERno âmbito do programa PT2020 pelo apoio financeiro ao CIMO (UIDI AGR/00690/2013). D. Mina agradece à FCT,pela bolsa de doutoramento SFRH1BD/105341 12014., info:eu-repo/semantics/publishedVersion
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- 2017
218. Pragas
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Santos, Sónia A.P., Bento, Albino, and Pereira, José Alberto
- Abstract
info:eu-repo/semantics/publishedVersion
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- 2017
219. Ability of fungi isolated from olive tree cultivars with different susceptibilities to olive knot to inhibit Pseudomonas savastanoi pv. savastanoi
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Gomes, Teresa, Pereira, José Alberto, Lino-Neto, T., Baptista, Paula, Castellano, Juan, and Universidade do Minho
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Ciências Naturais::Ciências Biológicas ,Olive knot disease ,Fungi ,Phyllosphere ,Endophytes and epiphytes ,Olive tree ,Oleae europaea - Abstract
The Olive Knot is distributed in olive-growing regions worldwide. This disease is caused by the Pseudomonas savastanoi pv. sovastonoí (Psv), a bacterium that produces galls especially on stems and twigs of olive tree. The use of biological control agents (BCAs)to control plant diseases have gained increasing attention during the last decades, due the negative impact of pesticides on environment and human health. This strategy can offer an alternative in order to achieve a sustainable and environ mentally friendly agriculture. The knowledge of fungal communities inhabiting the phyllosphere of olive tree and the type of interaction that they established with the host plant is essential to explore the use of these microorganisms as BCAs against Olive Knot. Previously, we have characterized the fungal communities inhabiting either the surface (epiphytes) or internal tissues of twigs (endophytes)from olive tree cultivars with different susceptibilities to Olive Knot (Cobrançosa moderately tolerant; Verdeal Transmontana susceptible). In this work, 43 endo- and epiphytes of this fungal community were screened for the suppression of Psv, and several mechanisms behind this activity were also studied by evaluating lytic enzymes production. The interspecific interaction was assessed on two different culture media, potato dextrose agar (PDA)and olive leaf twigs extract (OlTE), with agar overlays and by measuring the zone of growth inhibition. Lytic enzymes were evaluated qualitatively. In general, 95% of the fungal species tested inhibited significantly Psv and above an inhibition index (11) threshold of 1.5, being Dothiorella iberica, Rhinocladiella similis, Phoma aloes and Quambalaria cyanescens the species thatexhibited the maximum values (11 ranging from 31 to 19).The inhibition was observed to be affected by growth medium and correlated with the cultivar from which fungi were isolated. In OlTE was observed higher growth inhibition (up to 1.7 fold) and number of species with 11> 5 (up to 1.9 fold), compared to medium without host plant extracts. Similarly, fungal species isolated from the tolerant cultivar Cobrançosa showed higher capacity to inhibit Psv (up to 1.3 fold) when compared to isolates from cv.Verdeal Transmontana. This effect was noticed either for endophytes or epiphytes. The production of lipase, protease, amylase, and cellulase by fungi contributed to the antagonistic activity against Psv, whose production was influenced by olive tree extracts. Overall, results revealed that intimate physical interaction between fungal endophyte/epiphyte with Psvin the presence of olive tree extracts leads to the production of fungal secondary metabolite compounds that inhibit the pathogen growth, which were otherwise not produced. The potential of these fungal species as biological control agents should be considered and further evaluated under "in vivo" conditions., This work is funded by FEDER funds through COMPETE (Programa Operacional Factores de Competitividade) and by national funds by FCT (Fundação para a Ciência e a Tecnologia) in the framework of the project EXCL/AGR-PRO/0591/2012., This work is funded by FEDERfunds through COMPETE(Programa Operacional Factores de Competitividade) and by national funds by FCT(Fundação para a Ciência ea Tecnologia) inthe framework of the project EXCL/AGR-PRO!0591/2012.T. Gomes thanks FCT, POPH-QRENand FSE for PhD SFRH/BD/98127/2013 Grant., info:eu-repo/semantics/publishedVersion
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- 2017
220. Endophytic and epiphytic fungal community associated to olive tree differ in antagonistic activity against Pseudomonas savastanoi pv. savastanoi
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Gomes, Teresa, Pereira, José Alberto, Lino-Neto, T., Baptista, Paula, and Universidade do Minho
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Ciências Naturais::Ciências Biológicas ,Olive knot disease ,Epiphyte ,Pseudomonas savastanoi ,Endophyte ,Antagonistic activity ,Oleae europaea - Abstract
The Olive knot (OK) caused by the Pseudomonas savastanoi pv. savastanoi (Psv) is an important disease, causing severe damages and yield losses in olive trees worldwide (Olea europaea L.). In a previous study we have isolated this bacterium from the phyllosphere of olive tree, together with many fungal species. In these complex communities, microorganisms compete with each other for space and resources, promoting survival of the best-adapted individuals. This has prompted interest in the exploitation of these microorganisms for OK control. In this study, 48 fungal species from the endo- and epiphytic communities of olive twigs were screened for the growth inhibition of the phytopathogen Psv under in vitro conditions. The time course of interspecific interactions (24, 48, 72 and 144h) was studied in potato dextrose agar and olive leaf + twig extract (OLTE), by assessing a clear zone of growth inhibition around fungal colony. Results showed that epiphytic community was the main reservoirs for antagonistic fungi. Almost 75% of the tested epiphytes inhibited Psv growth, being Dothiorella iberica, Aspergillus felis and Aspergillus brasiliensis the most prominent species. The proportion of antagonists within endophytic community was lower (11%), being the most efficient Epicoccum nigrum and Rhinocladiella similis. The antibacterial activity was observed to be significantly (p, info:eu-repo/semantics/publishedVersion
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- 2017
221. Effect of host plant and olive Ieaf spot disease on fungal community assembly in olive tree phyllosphere
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Gomes, Teresa, Pereira, José Alberto, Lino-Neto, T., Bennett, Alison E., Baptista, Paula, and Universidade do Minho
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Ciências Naturais::Ciências Biológicas ,fungi ,Olive knot spot ,Fungal community ,food and beverages ,Phyllosphere ,Oleae europaea - Abstract
Olive leaf spot (OLS), a disease that usually occurs on the upper surface of the olive leaf, is caused by the fungus Fusicladium oleagineum with an ability to grow epiphytically and to enter inner leaf tissues. ln this work was studied the effect that host plant, at different levels (i.e. at cultivar level, in resistance to the fungal pathogen, and F. oleogineum infection), have on the phyllosphere fungal community composition of olive tree. For this, both epiphytic and endophytic fungal community inhabiting OLS-symptomatic and asymptomatic leaves of three co-occurring cultivars with different susceptibilities to OLS were compared, after isolation and identification of species by ITS rDNA sequencing. The results indicate that the presence of OLS on leaves had a major effect on the fungal community composition than the host plant, either at cultivar level or of their resistance to the fungal pathogen. Both diversity and abundance of epiphytes in asymptomatic leaves were significantly higher (up to 2.O-fold) than in symptomatic leaves, Although less pronounced, this trend was also evident in endophytes of two of the cultivars surveyed. Among the species identified in asymptomatic leaf, Cladosporium clodosporÍoides and Chromelosporium carneum were the most frequently isolated within epiphytic and endophytic fungal communities, respectively; whereas in symptomatic leaf, Cladosporium sp, and Pseudocercosporo cladosporioides, were the most frequently isolated within epiphytic and endophytic fungal communities, respectively. Determination of indicator value indices has shown significant preference/specificity of four species with asymptomatic leaves, and of two species with symptomatic leaves. Based on this analysis both Atternorio sp. and Tricharina striisporo seems to be the species with the most potential for OLS disease suppression. Future research needs to decipher these complex and dynamic interaction networks and assess their role in plant health., info:eu-repo/semantics/publishedVersion
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- 2017
222. La glucofagia favorece la supervivencia y afecta al comportamiento durante la selección de alimentos 'no presa' en dos grupos funcionales de arañas
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Benhadi-Marín, Jacinto, Pereira, José Alberto, Villa, María, Sousa, José Paulo, and Santos, Sónia A.P.
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Aranhas - Abstract
Las arañas han sido tradicionalmente consideradas carnívoros estrictos. Sin embargo, la evidencia sobre la capacidad de las arañas para alimentarse de recursos florales como el néctar y el polen está aumentando constantemente. La glucofagia en arañas puede aumentar su longevidad y fitness estando las señales visuales y olorosas implicadas en el comportamiento durante la alimentación. El objetivo de este trabajo fue evaluar el efecto de diferentes alimentos no presa en la supervivencia de dos grupos funcionales de arañas y estudiar su comportamiento de selección de estos alimentos. Las especies modelo seleccionadas fueron Haplodrassus severus (C.L. Koch) (Gnaphosidae) y Synema globosum (Fabricius) (Thomisidae). Como alimentos no presa se utilizaron disoluciones de diferentes azúcares a diferentes concentraciones, una mezcla de glucosa y aminoácidos, miel, pólen, levadura de cerveza, así como melaza de Saissetia oleae (Olivier) y Aphis craccae Linnaeus. Se diseñaron dos tipos de experimento: (1) ensayos de longevidad con un único alimento y (2) ensayos de selección múltiple con varios alimentos. En general, los alimentos “no presa” aumentaron significativamente la supervivencia global de las arañas de ambas especies comparados con el control. El máximo valor de longevidad fue de 117 días alcanzando por un individuo de H. severus alimentado con melaza de S. oleae. Cuando se ofrecieron juntos, ambas especies escogieron de forma diferente entre los alimentos no presa. H. severus prefirió la miel, mientras que S. globosum prefirió la mezcla de glucosa con aminoácidos. Considerando los alimentos utilizados en ambos tipo de experimento simultáneamente, las dos especies prefirieron el alimento que les proporcionó la mayor longevidad. Las arañas se benefician de los nutrientes obtenidos no sólo de las presas sino también de los alimentos no presa, que se buscan y explotan activamente. Este aporte alternativo de nutrientes puede ser especialmente importante durante los primeros estadios de desarrollo. info:eu-repo/semantics/publishedVersion
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- 2017
223. La interacción entre presas afecta a la respuesta funcional de las arañas y a su preferencia por los distintos tipos de presa
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Marín, Jacinto Benhadi, Pereira, José Alberto, Barreales, David, Sousa, José Paulo, and Santos, Sónia A. P.
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- 2017
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224. Screening of potential biocontrol bacterial against Pseudomonas savastanoi pv. savastanoi and elucidation of their mode of action
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Mina, Diogo, Pereira, José Alberto, Lino-Neto, T., Baptista, Paula, and Universidade do Minho
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Bacterial endophytes and epiphytes ,Ciências Naturais::Ciências Biológicas ,Olive knot disease ,Biological control ,food and beverages ,Pseudomonas savastanoi - Abstract
Over the last decades, the olive knot disease, caused by the bacterium Pseudomonas savastanoi pv. savastanoi (Psv), has been responsible for irreversible damages on olive orchards. Reduced vigor and stem dryness caused by this phytopathogen lead to a decrease in olive fruit production, conducting to countless losses for farmers. In this work, bacterial endophytes and epiphytes of olive tree phyllosphere were screened for the suppression of Psv, and several mechanisms behind this activity was also studied by evaluating indoleacetic acid (IAA), siderophore and lytic enzymes production. Interspecific interaction was assessed on solid media with agar overlays. IAA was estimated spectrophotometrically, whereas siderophores and lytic enzymes were evaluated qualitatively. Several bacterial species tested showed to reduce Psv growth up to 70%, as well as its viability. The highest inhibition was observed for Frondihabitans sp. and Paenibacillus sp. A reduction on production of both IAA and siderophore, which are associated with knot development, by Psv was noticed in the presence of the most efficient bacterial. Production of lytic enzymes by antagonists such as lipase, chitinase, protease and amylase was also identified. Altogether the results indicate that some of the bacterial tested have great potential as biocontrol agents due to their capacity to produce metabolites/lytic enzymes that can interfere with Psv growth and/or development of knots. These potential biological agents should be further evaluated under natural conditions., This work is funded by FEDER funds through COMPETE (Programa Operacional Factores de Competitividade) and by national funds by FCT (Fundação para a Ciência e a Tecnologia) in the framework of the project EXCL/AGR-PRO/0591/2012. D. MINA thanks the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant SFRH/BD/105341/2014, info:eu-repo/semantics/publishedVersion
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- 2017
225. Unexplored olive cultivars from the Valencian Community (Spain): some chemical characteristics as a valorization strategy
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Salazar-García, Domingo C., primary, Malheiro, Ricardo, additional, Pereira, José Alberto, additional, and Lopéz-Cortés, Isabel, additional
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- 2018
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226. Effects of different drying methods on the bioactive compounds and antioxidant properties of edible Centaurea (Centaurea cyanus) petals
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Fernandes, Luana, primary, Casal, Susana, additional, Pereira, José Alberto, additional, Saraiva, Jorge Alexandre, additional, and Ramalhosa, Elsa, additional
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- 2018
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227. Use of Response Surface Methodology (RSM) for the Identification of the Best Extraction Conditions for Headspace Solid‐Phase Micro Extraction (HS‐SPME) of the Volatile Profile of cv. Arbequina Extra‐Virgin Olive Oil
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Borges, Thays H., primary, Ramalhosa, Elsa, additional, Seiquer, Isabel, additional, and Pereira, José Alberto, additional
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- 2018
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228. Optimization of osmotic dehydration of chestnut (Castanea sativa Mill.) slices using Response Surface Methodology
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Delgado, Teresa, primary, Paim, Bruna, additional, Pereira, José Alberto, additional, Casal, Susana, additional, and Ramalhosa, Elsa, additional
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- 2018
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229. Effect of hot air convective drying on sugar composition of chestnut (Castanea sativa Mill.) slices
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Delgado, Teresa, primary, Pereira, José Alberto, additional, Ramalhosa, Elsa, additional, and Casal, Susana, additional
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- 2018
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230. Organic acid profile of chestnut (Castanea sativa Mill.) as affected by hot air convective drying
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Delgado, Teresa, primary, Ramalhosa, Elsa, additional, Pereira, José Alberto, additional, and Casal, Susana, additional
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- 2018
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231. Physicochemical composition and antioxidant activity of several pomegranate (Punica granatum L.) cultivars grown in Spain
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Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal, Interreg, European Regional Development Fund, Fernandes, Luana, Pereira, José Alberto, López- Cortés, I., Salazar Hernández, Domingo Manuel, González-Álvarez, Julia, Ramalhosa, Elsa, Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal, Interreg, European Regional Development Fund, Fernandes, Luana, Pereira, José Alberto, López- Cortés, I., Salazar Hernández, Domingo Manuel, González-Álvarez, Julia, and Ramalhosa, Elsa
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[EN] Nine pomegranate cultivars grown in Spain were selected, and their physicochemical (total soluble solids, pH, titratable acidity, maturity index, monomeric anthocyanin pigment, flavonoids, hydrolyzable tannins, and vitamin C) and antioxidant properties and polyphenolic composition of the juices were compared. A total of 53 polyphenols were identified, showing cultivars different profiles. Of all nine cultivars, Katirbasi had the highest contents of flavonoids, hydrolyzable tannins and vitamin C, as well as gallic acid and ellagic acid contents, explaining its high total reducing capacity. Principal component analysis allowed Katirbasi to be differentiated clearly from the others. Other cultivars presented also interesting characteristics such as high monomeric anthocyanin pigment content (CG8 cultivar) and interesting antioxidant activity (Wonderful 2 and CG8 cultivars). CG8 was the cultivar with the highest value of cyanidin-3,5-di-O-glucoside. Thus, this study will assist pomegranate producers in choosing the most suitable cultivar according to its ultimate use.
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- 2017
232. Caracterização físico-química e sensorial de doces de fruta de São Tomé e Príncipe
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Vollero, Agostino, Fernandes, Luana, Delgado, Teresa, do Céu Fidalgo, Maria, Pereira, José Alberto, Ramalhosa, Elsa, Vollero, Agostino, Fernandes, Luana, Delgado, Teresa, do Céu Fidalgo, Maria, Pereira, José Alberto, and Ramalhosa, Elsa
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Introduction: São Tomé and Príncipe has a wide variety of fruit trees, being most of the fruits consumed in fresh. In certain periods of the year, fruits are available at significant amounts; however, it is not common to use the surplus of those fruits. Thus, this excess may be used in the preparation of other fruit based products (ex. jams), in order to increase product diversity and allow the annual consumption of these seasonal fruits. Objetives: Valorise fruits’ production in S. Tomé and Príncipe. Methods: Preparation of jams of banana, ambarella, guava (dark and light) and papaya, with two levels of sugar, and perform their physico-chemical characterization (color, pH, moisture and acidity). Furthermore, sensory analysis was also done, through preference and acceptability tests. Results: The jams prepared had different colours, acidity values, and moisture and ash contents, demonstrating the possibility of producing different products by changing the formulation. Regarding jams preference, 60% of the consumers preferred the less sweet in the case of banana and guava (light), whereas, over 67% of the panellists preferred the sweetest ambarella jam. Concerning dark guava and papaya, the percentages were similar for both sugar contents. For all attributes analysed (appearance, colour, taste, acidity, sweetness and global evaluation), most of the panellists liked slightly all jams. Conclusion: Jams production in S. Tomé and Príncipe may be a promising activity., Introdução: São Tomé e Príncipe tem uma grande variedade de árvores frutíferas, sendo a maioria dos frutos consumida em fresco. Em certos períodos do ano, os frutos estão disponíveis em quantidades significativas, não sendo comum usar o excedente. Assim, o excedente de frutos pode ser usado na preparação de outros produtos (ex. doces), para aumentar a diversidade de produtos e permitir o consumo anual desses frutos sazonais. Objetivos: Valorizar a produção de frutos de S. Tomé e Príncipe. Métodos: Elaboração de doces de banana, cajá-manga, goiaba (escura e clara) e mamão, com diferentes teores de açúcar, e posteriormente proceder à sua caracterização físico-química (cor, pH, teor de humidade e acidez) e sensorial, através de testes de preferência e aceitabilidade. Resultados: Os doces preparados apresentaram diferentes cores e valores de acidez, humidade e cinza, demonstrando a possibilidade de elaboração de diferentes produtos pela modificação da formulação. Quanto à preferência dos doces, 60% dos consumidores preferiram os menos doces no caso da banana e goiaba (clara), enquanto mais de 67% dos participantes preferiram o doce de cajá- manga mais doce. Quanto à goiaba escura e papaia, as percentagens foram semelhantes para ambos os teores de açúcar. Para todos os atributos analisados (aparência, cor, sabor, acidez, doçura e avaliação global), a maioria dos participantes gostou ligeiramente de todos os doces. Conclusões: A produção de doces em S. Tomé e Príncipe pode ser uma actividade promissora, Introducción: S. Tomé y Príncipe tiene una gran variedad de árboles frutales, siendo la mayoría de los frutos comidos en fresco. En ciertos períodos del año, los frutos están disponibles en cantidades significativas, no siendo común el uso de los excedentes. Por lo tanto, este excedente se puede utilizar en la preparación de otros productos y permitir el consumo anual de la fruta de temporada. Objetivos: Valorar la producción de frutos en S. Tomé y Príncipe. Métodos: Preparación de mermelada de plátano, caja-mango, guayaba (clara y oscura) y papaya con distintos contenidos de azúcar, y proceder à su caracterización físico-química (color, pH, contenido de humedad y acidez) y sensorial, a través de pruebas de preferencia y aceptabilidad. Resultados: Las mermeladas preparadas tenían diferentes colores y valores de acidez, humedad y cenizas, lo que demuestra la posibilidad de producir diferentes productos. En relación à la preferencia de las mermeladas, 60% de los consumidores prefiere la menos dulce en el caso del plátano y guayaba (clara), mientras más del 67% de los participantes prefiere la mermelada de caja-mango más dulce. En cuanto a la guayaba oscura y papaya, los porcentajes fueron similares para ambos niveles de azúcar. Para todos los atributos analizados (aspecto, color, sabor, acidez, dulzor y evaluación global), la mayoría de los participantes les gustó un poco todas las mermeladas. Conclusións: La producción de mermeladas en S. Tomé y Príncipe puede ser una actividad prometedora.
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- 2017
233. 4 - Olive Oil Phenolic Composition as Affected by Geographic Origin, Olive Cultivar, and Cultivation Systems
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Malheiro, Ricardo, Rodrigues, Nuno, and Pereira, José Alberto
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- 2015
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234. Improvement of sensorial and volatile profiles of olive oil by addition of olive leaves
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Malheiro, Ricardo, primary, Rodrigues, Nuno, additional, Bissaro, Camila, additional, Leimann, Fernanda, additional, Casal, Susana, additional, Ramalhosa, Elsa, additional, and Pereira, José Alberto, additional
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- 2017
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235. Cooking impact in color, pigments and volatile composition of grapevine leaves (Vitis vinifera L. var. Malvasia Fina and Touriga Franca)
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Lima, Adriano, primary, Pereira, José Alberto, additional, Baraldi, Ilton, additional, and Malheiro, Ricardo, additional
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- 2017
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236. Characterization of Arbequina virgin olive oils produced in different regions of Brazil and Spain: Physicochemical properties, oxidative stability and fatty acid profile
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Borges, Thays H., primary, Pereira, José Alberto, additional, Cabrera-Vique, Carmen, additional, Lara, Luis, additional, Oliveira, Adelson F., additional, and Seiquer, Isabel, additional
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- 2017
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237. Avaliação do teor em compostos fenólicos e atividade antioxidante de folhas de videira com vista ao seu aproveitamento para uso alimentar
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Lima, Adriano Freitas, primary, Bento, Albino, additional, Pereira, José Alberto, additional, Baraldi, Ilton José, additional, and Malheiro, Ricardo, additional
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- 2017
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238. Elaboração de doces de frutos de São Tomé e Príncipe com vista à sua valorização
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Sousa, Agostinho Vaz De, Pereira, José Alberto, Delgado, Teresa, Fidalgo, Maria Do Céu, and Ramalhosa, Elsa
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São Tomé e Príncipe ,Análise organolética ,Caracterização físico-química ,Doces - Abstract
São Tomé e Príncipe é um país rico e diverso em frutas tropicais, que são apenas consumidas in natura, sendo rara a sua transformação. Nesse sentido, numa perspetiva de valorização dos recursos endógenos, no presente trabalho procedeu-se à formulação de doces com dois teores de açúcar (1:0,6 e 1:0,8, massa polpa:massa açúcar) a partir de frutas abundantes no País (banana, cajá-manga, goiaba - escura e clara - e mamão), e à sua caracterização físico-química e organolética. Os resultados indicaram, para as frutas estudadas, que os rendimentos de polpa foram superiores a 70%, apresentando os doces diferentes valores de pH, cor, acidez e teor de humidade e cinzas. Na maioria dos casos, os doces preparados com diferentes teores de açúcar apresentaram também características distintas. Na avaliação organolética dos doces, para o caso da goiaba clara e banana, mais de 60% dos consumidores preferiram os menos doces, para a cajá-manga a tendência foi contrária com mais de 67% dos provadores a preferirem o mais doce, e na goiaba escura e mamão não foi registada preferência no que toca à concentração de açúcar. Relativamente à aceitabilidade, não se detetaram diferenças significativas entre os doces que contêm diferentes teores de açúcar. O presente trabalho demonstrou que a produção de doces de frutas tropicais contribui para a valorização dos recursos locais e pode ser uma atividade promissora para aumentar o rendimento da população local. Several tropical fruits may be found in São Tomé and Príncipe Island; however, their transformation is not a common practice. In this way, it will be of great advantage for the local population to find alternatives to valorize this resource. The main objectives of the present work were to proceed to the formulation of jams with two levels of sugar (1:0.6 and 1:0.8, fruits pulp weight: sugar weight) from some tropical fruits of São Tomé and Príncipe, including banana, cajá-manga, guava (dark and light) and papaya, and to proceed to their physico-chemical and organoleptic characterization. The results obtained indicated that the pulp yields of the studied fruits were higher than 70%, presenting jams different pH values, color, acidity, and moisture and ash contents. In most cases, the jams prepared with different sugar contents also showed different characteristics. Concerning preference, 60% of the consumers preferred the less sweet jam in the case of banana and guava (light), while over 67% of the panelists preferred the sweetest cajá-manga jam. In the case of dark guava and papaya, the percentages were similar. Regarding jams acceptability, no significant differences were found between jams prepared with different sugar amounts. In conclusion this work showed that jams production from tropical fruits of São Tomé and Príncipe may be a promising activity for the local population.
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- 2015
239. EcoPred: an educational individual based model to explain biological control, a case study within an arable land
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Benhadi-Marín, Jacinto, Pereira, José Alberto, Sousa, José Paulo, and Santos, Sónia A.P.
- Abstract
ABSTRACTIndividual based models (IBMs) are up-to-date tools both in research and educational areas. Here we introduce an IBM built on NetLogo platform that simulates a top-down trophic cascade controlled by the pressure exerted by two model predators (web-building spiders and ground runner spiders) on a model pest (the olive fruit fly) within a hypothetical agricultural landscape (the olive crop). EcoPred is an IBM that aims to be an educational tool that can help teachers to explain concepts related to ecology in a modern, enjoyable and comprehensive way. EcoPred reflects the changes on a fly population within a simulated olive crop according to (1) the mortality rate caused by the predation of two spider species and energy loss, (2) the energy gain by feeding on flowers and (3) the reproduction rate in olive trees. The model was tested with 26 students achieving very good results in terms of acceptance and interest on the learning method. EcoPred can be used for educational purposes with 16 year old students and older to explain ecological concepts such as trophic level, species interactions, limiting factor and biological control in an interactive way simultaneously introducing students to biology oriented programming languages.
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- 2020
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240. Application of chemometric tools for the comparison of volatile profile from raw and roasted regional and foreign almond cultivars (Prunus dulcis).
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Oliveira, Ivo, Malheiro, Ricardo, Meyer, Anne S., Pereira, José Alberto, and Gonçalves, Berta
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In almonds, volatile compounds are major contributors to flavour, being scarce the current knowledge about their volatile profile. Hence, this work intended to characterize the volatile profile, using headspace solid-phase microextraction and gas chromatography–mass spectrometry, in raw and roasted almond cultivars (regional cvs. Amendoão, Bonita, Casanova, Molar and Pegarinhos and foreign cvs. Ferragnès and Glorieta). Overall, 35 compounds were identified, with major chemical classes being alcohols and aldehydes. In raw fruits, benzaldehyde and 3-methyl-1-butanol were key compounds, with roasting changing volatile profiles, increasing release of compounds, with predominance of hexanal and benzaldehyde. Cultivars Glorieta and Molar didn't show significant increase in aldehyde content after roasting, which may indicate higher resistance to heat-caused oxidation. The use of linear discriminant analysis and principal components analysis permitted the recognition of patterns in the volatile profiles, that can be useful for cultivars identification. This work allowed the characterization and monitoring changes caused by roasting of volatile components of less studied almond cultivars, identifying some that can withstand roasting procedures with reduced formation of compounds associated with off-flavours. [ABSTRACT FROM AUTHOR]
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- 2019
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241. Spiders actively choose and feed on nutritious non-prey food resources.
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Benhadi-Marín, Jacinto, Pereira, José Alberto, Sousa, José Paulo, and Santos, Sónia A.P.
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SPIDERS , *PREDATORY insects , *AGRICULTURAL ecology , *POLLEN , *BIOLOGICAL pest control - Abstract
Highlights • Spiders are efficient predators in agroecosystems against pests. • Different non-prey foods significantly enhanced spiders' fitness. • Spiders actively selected the most beneficial non-prey foods. • Crops can offer alternative foods for spiders. • Agricultural management should promote practices that provide non-prey foods. Abstract Spiders are generalist predators adapted to consume a wide range of prey although their ability to exploit non-prey foods such as pollen, nectar, and honeydew has been referred but less studied. In this work, we investigated the effect of different non-prey food items (Glucose at 0.5 M; aphid honeydew; black scale honeydew; a mixture of glucose 0.5 M, phenylalanine 0.1 mM, proline 0.1 mM, and tryptophan 0.1 mM; honey at 10%, and pollen at 10%) on the survival of immature spiders of two functional groups represented by Haplodrassus rufipes (ground hunters) and Synema globosum (ambushers), and their feeding choices, in laboratory experiments. The overall survival of both species fed on non-prey foods significantly increased compared to individuals fed on water. The black-scale honeydew was the best food for H. rufipes increasing longevity up to 117 days. The highest survival reached by S. globosum was observed when fed on a mixture of glucose 0.5 M and three amino acids. When different non-prey food items were offered together, the exploring rate was significantly higher for H. rufipes than for S. globosum. H. rufipes chose to feed on honey whereas S. globosum chose the mixture treatment. The most chosen food items corresponded with those that provided the highest longevities in both species. Our results suggest that spiders could search, recognize and actively select the most beneficial non-prey food. Habitat management practices such as maintaining weed strips in the crop may provide these valuable supplementary food resources within agroecosystems contributing for biological pest control. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
242. Unexplored olive cultivars from the Valencian Community (Spain): some chemical characteristics as a valorization strategy.
- Author
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Salazar-García, Domingo C., Malheiro, Ricardo, Pereira, José Alberto, and Lopéz-Cortés, Isabel
- Subjects
OLIVE oil ,OLIVE varieties ,VITAMIN E ,UNSATURATED fatty acids ,LINOLEIC acid - Abstract
The olive processing industry has till date been dominated by a small group of cultivars, leading to the possibility of some olive cultivars becoming extinct in the near future. In this study, we determined the composition of some chemical components in the olive oils from 31 minor olive cultivars of the Valencian Community. Our main aim was to identify suitable cultivars, which could produce differentiated olive oils, thus aiming towards their valorization. The average oil content of minor olive cultivars was found to be good, with some of them reporting approximately 60% (dry basis). On average, the total phenolic content was 229 mg kg
− 1 , with cv. Mas Blanc reporting the highest content (570 mg kg− 1 ). Among the various tocopherols found in olives, α-tocopherol was the main constituent, with a maximum concentration of 290.6 mg kg− 1 . Linoleic acid was the main polyunsaturated fatty acid and varied between 3.4% (cv. Del Pomet) and 16.9% (cv. Blanqueta Enguera). Special attention needs to be paid to the composition of sterols, since some olive oils exceeded the limits established for some sterols by the current European legislation. Some of the cultivars studied were highly productive, and originated differentiated olive oils with a rich composition of antioxidants and essential fatty acids. In some cases, these beneficial compounds were higher than those of commercial oils obtained from the most common cultivars worldwide. These results could contribute to the commercial exploitation of some of the studied cultivars. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
243. Functional dependence at discharge from the intensive care unit: relevance for rehabilitation nursing.
- Author
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Dias da Silva Azevedo, Paulo Manuel, Pereira Gomes, Bárbara, Teixeira Pires Pereira, José Alberto, Nascimento Carvalho, Flávia Miranda, Camelo Ferreira, Soraia Patricia, Pires, Ana Isabel, and Macedo, José
- Subjects
CRITICALLY ill ,LENGTH of stay in hospitals ,INTENSIVE care units ,LIFE skills ,RESEARCH methodology ,PATIENTS ,REHABILITATION nursing ,RESEARCH ,STATISTICAL sampling ,HEALTH self-care ,QUANTITATIVE research ,DISCHARGE planning ,DESCRIPTIVE statistics - Abstract
Copyright of Revista de Enfermagem Referência is the property of Escola Superior de Enfermagem de Coimbra and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
- Full Text
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244. Syrphids feed on multiple patches in heterogeneous agricultural landscapes during the autumn season, a period of food scarcity
- Author
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López Sáez, José Antonio [0000-0002-3122-2744], Villa, María, Santos, Sonia A. P., Marrão, Rosalina, Pinheiro, Lara A., López Sáez, José Antonio, Mexia, Antonio, Bento, Albino, Pereira, José Alberto, López Sáez, José Antonio [0000-0002-3122-2744], Villa, María, Santos, Sonia A. P., Marrão, Rosalina, Pinheiro, Lara A., López Sáez, José Antonio, Mexia, Antonio, Bento, Albino, and Pereira, José Alberto
- Abstract
Many syrphid larvae are predators and have an important role as biological control agents of pests in agroecosystems. However, adults feed on non-prey resources such as pollen from flowers. Heterogeneous landscapes can provide syrphids with a great biodiversity of plants and ensure the existence of food resources. This is particularly important during periods of food scarcity, such as autumn, for syrphid species that spend those periods as adults. Nevertheless, the feeding habits of syrphid adults in resource-scarce agroecosystems are poorly known. In this study, the pollen consumption and preferences of Eupeodes corollae (Fabricius) and Episyrphus balteatus (De Geer) were analyzed in olive groves and surrounding herbaceous and woody patches in the autumns of 2012 and 2013 in northeastern Portugal. The guts were dissected, and the pollen types were identified and compared with the ground cover plants in the studied patches. Both species consumed and selected pollen types from herbaceous and woody vegetation that occurred in different patches, indicating that they flew between patches. These results highlight the importance of conserving heterogeneous agricultural landscapes to guarantee the existence of food resources for beneficial insects during periods of scarcity.
- Published
- 2016
245. Influence of sustained deficit irrigation and foliar kaolin application on almond kernel composition.
- Author
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Barreales, David, Pereira, José Alberto, Casal, Susana, and Ribeiro, António Castro
- Subjects
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DEFICIT irrigation , *ALMOND , *KAOLIN , *WATER levels , *IRRIGATION water , *ESSENTIAL oils - Abstract
• Effects of sustainable deficit irrigation combined with foliar kaolin application on physical-chemical almond characteristics. • Morphological proprieties, volatile compounds, fatty acids profile and tocopherols and tocotrienols compounds were analysed. • Sustainable deficit irrigation improves the water status of the almond trees without reducing fruit quality. • No effects were observed with the foliar kaolin application. Deficit irrigation and kaolin foliar application are agronomic practices that have been demonstrated to improve productivity and physiological response in almond trees but there is no consistent information on the effects on the kernel composition. The objective of this study was to evaluate the effect of different Sustainable Deficit Irrigation (SDI) strategies and kaolin application on some physicochemical composition of the cv Ferragnès almond (Prunus dulcis (Mill.) D.A. Webb). A randomized block design with five treatments was developed: non-irrigation (NI), non-irrigation with foliar kaolin application (NI+K), full irrigation (FI), and two levels of SDI receiving 70% and 35% of evapotranspiration (SDI75 and SDI35). Pomological parameters, volatile profile and oil composition were analyzed. SDI effect on kernel weight was reduced against full irrigation (FI) but substantially improved in comparison with the rainfed treatments (NI and NI+K). Irrigation treatments showed a lower volatile compounds concentration in comparison with rainfed treatment. The fatty acid composition of the oil was significantly affected, with all the irrigated treatments having higher oleic acid contents than the rainfed treatments, and therefore an expected increased shelf life. Kaolin application had no positive effects on the non-irrigated trees, other than an apparent increase in the total volatiles, a tendency also observed in the NI. Moderate water stress levels (SDI35) improved the synthesis of tocopherols compared to NI, FI and SDI70, which could be related to the adaptation of the almond tree to drought. SDI35 is therefore regarded as a very interesting approach, with significant improvements in comparison with non-irrigated trees, and a clear saving on water against full irrigation without significant pomological and chemical alterations. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
246. Physico-chemical and organoleptic characterization of fruit jams of São Tomé and Príncipe
- Author
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Ramalhosa, Elsa, Sousa, Agostinho Vaz De, Delgado, Teresa, Fidalgo, Maria Do Céu, and Pereira, José Alberto
- Published
- 2014
- Full Text
- View/download PDF
247. Influência das cascas e películas nas cinéticas de secagem de castanhas (Castanea sativa Mill.)
- Author
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Delgado, Teresa, Pereira, José Alberto, Casal, Susana, and Ramalhosa, Elsa
- Published
- 2014
- Full Text
- View/download PDF
248. Efeito da desidratação osmótica nas propriedades físico-químicas de castanha fatiada
- Author
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Delgado, Teresa, Paim, Bruna, Pereira, José Alberto, Casal, Susana, Ramalhosa, Elsa, and Faculdade de Farmácia
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Teor de humidade ,Castanea sativa Miller ,Metodologia de superfície de resposta ,Longal ,Ciências agrárias ,Cor ,Ganho de sólidos ,Agrarian Sciences - Abstract
A castanha é um produto com elevada importância económica para alguns países. A castanha é um fruto sazonal, a qual apresenta alguns inconvenientes no seu armazenamento, tais como, a fácil perda de peso e o desenvolvimento de fungos. Deste modo, é importante arranjar alternativas para a conservação deste produto. A desidratação osmótica tem sido bastante usada para preservar alimentos perecíveis e facilitar a sua comercialização em regiões distantes da sua zona de produção. Na desidratação osmótica a temperatura, concentração de solutos e tempo são variáveis a considerar. A fim de combinar e analisar o papel de alguns destes fatores e minimizar os erros de análise, foram desenvolvidas técnicas estatísticas e matemáticas a fim de melhorar e otimizar processos. A Metodologia de Superfície de Resposta (Response Surface Methodology) é uma dessas técnicas onde se tenta avaliar a influência de variáveis independentes (fatores) nas respostas de interesse. Além de ter a vantagem de analisar os efeitos das variáveis independentes e da sua interação, esta metodologia gera um modelo matemático que descreve os processos químicos ou bioquímicos a estudar. O objetivo do presente trabalho foi o de avaliar o papel da temperatura (30; 45 e 60 ºC), tempo (2,5; 5 e 7,5 h) e concentração da solução osmótica (60, 70 e 80% de sacarose) sobre algumas propriedades físico-químicas da castanha (variação de cor, perda de peso, ganho de sólidos, perda de água, teor de humidade e teor de humidade normalizado). Os modelos obtidos para estas propriedades apresentaram bons ajustes com coeficientes de determinação (R2) entre 0,850 e 0,976 e um R2-ajustado entre 0,715 e 0,954, permitindo prever os dados experimentais com bastante rigor. Verificou-se que a temperatura e a concentração de soluto tiveram um papel importante em todas as propriedades analisadas. Contudo, a concentração de soluto não influenciou significativamente a variação de cor. Já o tempo foi um fator importante para a variação de cor, ganho de sólidos, teor de humidade e teor de humidade normalizado. Tal como previsto, foram observadas maiores variações de cor a temperaturas e tempos mais elevados. O teor de humidade e o teor de humidade normalizado foram maiores para concentrações de soluto e temperaturas menores. Observou-se também uma maior perda de peso e maior ganho de sólidos, a concentrações de soluto, temperaturas e tempos mais elevados. A maior perda de água foi observada para as concentrações de soluto e temperaturas mais elevadas, não sendo afetada pelo tempo.
- Published
- 2014
249. Monitoring olive oils quality and oxidative resistance during storage using an electronic tongue
- Author
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Rodrigues, Nuno, primary, Dias, Luís G., additional, Veloso, Ana C.A., additional, Pereira, José Alberto, additional, and Peres, António M., additional
- Published
- 2016
- Full Text
- View/download PDF
250. Syrphids feed on multiple patches in heterogeneous agricultural landscapes during the autumn season, a period of food scarcity
- Author
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Villa, María, primary, Santos, Sónia A.P., additional, Marrão, Rosalina, additional, Pinheiro, Lara A., additional, López-Saez, Jose Antonio, additional, Mexia, António, additional, Bento, Albino, additional, and Pereira, José Alberto, additional
- Published
- 2016
- Full Text
- View/download PDF
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