251. Identification of metabolic profiling of cell culture of licorice compared with its native one.
- Author
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Man, Shuli, Guo, Songbo, Gao, Wenyuan, Wang, Juan, Zhang, Liming, and Li, Xinglin
- Subjects
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GLYCYRRHIZA , *SUSPENSIONS (Chemistry) , *METABOLIC profile tests , *FLAVONOIDS , *LIQUID chromatography-mass spectrometry - Abstract
Glycyrrhiza uralensis has long been used as a flavoring and sweetening agent in food products. In the last ten years, suspensions of Glycyrhiza cells have been successfully established. However, there is no report of full metabolic profiling research on these cells. To identify their composition we used HPLC-DAD coupled with ESI(+/−)-MS to compare the constituents of cultured Glycyrhiza (CG) cells with those the native cells (NG). We identified 60 compounds including flavonoids, phenols, and triterpenoids. Among these compounds, 42 occurred both in NG and CG, nine were present in NG only and nine were present in CG alone. The number of the triterpenoid aglycones without glycones in CG was smaller than that in NG. The number of flavanone, isoflavone, isoflavan, and benzenoid compounds was also smaller in CG than that in NG, whereas the number of pterocarpans was much higher. Although differences existed between CG and NG, the extract of CG was similar to that of NG. With the development of cell suspension culture-based biotransformation, cell culture of Glycyrrhiza has the potential to be more profitable than field cultivation in some areas. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
- Published
- 2013
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