251. Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications.
- Author
-
Yang Q, Rout MP, and Akey CW
- Subjects
- Animals, Freezing, Fungal Proteins chemistry, Fungal Proteins ultrastructure, Image Processing, Computer-Assisted, Microscopy, Electron, Scanning Transmission, Molecular Weight, Protein Conformation, Saccharomyces metabolism, Species Specificity, Vertebrates, Evolution, Molecular, Nuclear Envelope chemistry, Nuclear Envelope ultrastructure, Saccharomyces chemistry, Saccharomyces ultrastructure
- Abstract
We have calculated a three-dimensional map of the yeast nuclear pore complex (yNPC) from frozen-hydrated specimens, thereby providing a direct comparison with the vertebrate NPC. Overall, the smaller yNPC is comprised of an octagonal inner spoke ring that is anchored within the nuclear envelope by a novel membrane-interacting ring. In addition, a cylindrical transporter is located centrally within the spokes and exhibits a variable radial expansion in projection that may reflect gating. The inner spoke ring, a transmembrane spoke domain, and the transporter are conserved between yeast and vertebrates; hence, they are required to form a functional NPC. However, significant alterations in NPC architecture have arisen during evolution that may be correlated with differences in nuclear transport regulation or mitotic behavior.
- Published
- 1998
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