351. siRNA-mediated knockdown of FoxP3 promotes the ratio of T-helper 1 (Th1) to Th2 in chronic hepatitis B patients.
- Author
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Yongsheng Y, Xiaoliang L, Zhenghao T, and Guoqing Z
- Subjects
- Adult, Cell Proliferation, Cells, Cultured, Down-Regulation, Female, GATA3 Transcription Factor genetics, Gene Expression Regulation, Hepatitis B, Chronic genetics, Humans, Interferon-gamma metabolism, Interleukin-10 metabolism, Interleukin-2 metabolism, Interleukin-4 metabolism, Lymphocyte Count, Male, Middle Aged, RNA, Small Interfering, T-Box Domain Proteins genetics, Up-Regulation, Forkhead Transcription Factors genetics, Hepatitis B, Chronic blood, RNA, Messenger metabolism, T-Lymphocytes, Regulatory metabolism, Th1 Cells metabolism, Th2 Cells metabolism
- Abstract
To determine the effect of regulatory T cells on the ratio of Th1/Th2 differentiation in peripheral blood mononuclear cells of chronic hepatitis B patients, FoxP3, the essential transcription factor for Tcells differentiation and function, was knocked down by FoxP3 siRNA, which was affirmed by real-time polymerase chain reaction for decreased expression of FoxP3. Then, at different time points, comparing with control groups, we detected enhanced lymphocyte proliferation and up-regulated Th1-type cytokines, but down-regulated Th2-type cytokines by ELISA. Finally, to research the involved mechanisms, an increased ratio of T-bet/GATA-3 mRNA expression was found by real-time polymerase chain reaction. Our results suggest that siRNA-mediated knockdown of FoxP3 could regulate the immune balance of Th1/Th2 in chronic hepatitis B patients, which may be mediated partly by regulating transcription factors T-bet and GATA-3.
- Published
- 2011
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