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Aim. To investigate the influence of cultivation conditions on lysing activity of Bacillus amiloliquefaciens strain IMV B-7571. Methods. Lysing activity was determined by cocultivating the strain B. amiloliquefaciens IMV B-7571 and cyanobacterium Anabaena hassalii, Microcystis aeruginosa, M. pulverea in LB, NBY and glucose-mineral nutrient media. Determination of temperature and pH-optimum, study of the growth activity of strain B. amiloliguefaciens IMV B-7571 were studies by conventional methods. Results. The most lysis activity of strain B. amiloliguefaciens IMV B-7571 was at the 24th hour of its cultivation on LB medium. The level of lysis of test cultures cyanobacteria by the strain B. amiloliguefaciens IMV B-7571 was 54.76% for M. aeruginosa, 53.65% for M. pulverea and 58.33% for A. hassalii. The biological activity of strain B. amiloliguefaciens IMV B-7571 significantly depends on its cultivation temperature and pH. Lysing activity was recorded at a temperature of 35°C at pH 7.0. Conclusions. The obtained results will contribute to further research aimed at developing a biological biodrug with lysing activity based on strain B. amiloliguefaciens IMV B-7571. [ABSTRACT FROM AUTHOR]

Aim. Identification of Bacillus sp. 10.1 using phenotype characteristics and sequencing of 16S rRNA gene. Methods. Cultural, morphological, and physiological characteristics of the strain Bacillus sp. 10.1 were studied using guidelines of isolation and identification of bacteria of the genus Bacillus. Composition of fatty-acid of the cellular lipids were identified by the chromatography-mass spectrometry. The 16S rRNA genes were amplified by PCR using primers 27f and 1492r, according to standard protocol. Phylogenetic tree, constructed using the neighbour-joining method and using the model Kimura 2 parameter. Bootstrap analysis was applied using 100 bootstrap replicas. Results. The strain of Bacillus sp.10.1 inhibited the growth of cyanobacteria Anabaena hassalii, Microcystis aeruginosa, M. pulvereae. Cultural, morphological, and physiological characteristics of the strain Bacillus sp. 10.1 had been studied. It was showed that the studied strain belongs to the species B. amyloliquefaciens.To clarify the taxonomic position of the strain Bacillus sp. 10.1 had been studied the composition of fatty-acid of the cell wall and sequencing of 16S rRNA gene. Conclusions. Based on the analysis of nucleotide sequence of 16S rRNA gene the studied strain Bacillus sp. 10.1 had been classified as a B. velezensis. The sequence of 16S rRNA gene added to the database GenBank and accession number for the 16S rRNA of Bacillus sp. 10.1 are KH962173. [ABSTRACT FROM AUTHOR]

Silver-containing film materials are formed by vacuum sputtering of silver nanoparticles on the surface of polyelectrolyte complexes based on chitosan and sodium salt of carboxymethylcellulose (pectin). The obtained samples were characterized by the methods of wide-angle X-ray scattering and transmission electron microscopy, and their antimicrobial, antiviral and cytotoxic properties were also investigated. The presence of metallic silver on the surface of polyelectrolyte complexes was confirmed by the method of wide-angle X-ray diffraction. It was established that upon sputtering of silver, a ~200 nm thick layer is formed within 5 minutes. It was shown that Na-CMC--Ag--chitosan and pectin--Ag--chitosan samples, formed by silver sputtering, exhibit antimicrobial activity against test cultures of S. aureus, E. coli, P. aeruginosa, and C. albicans. Antiviral activity of samples against influenza A virus and herpes simplex virus type 1 was also established. The obtained samples were not cytotoxic, did not inhibit the viability of MDCK and BHK cell cultures. [ABSTRACT FROM AUTHOR]

Infectious diseases caused by viruses and microorganisms continue to be one of the biggest health problems worldwide, despite the rapid progress in the creation of drugs and the development of pharmaceutical technologies. Th e risk of complications and the spread of infections in society remains high. Scientifi c research and the fi ght against viral infections, in particular with a large group of respiratory diseases caused by viruses, such as infl uenza viruses, parainfl uenza viruses, respiratory sensitivities, adenoviruses, rhinoviruses, coronaviruses, etc., occupy an important place in modern medicine. Due to the nature, characteristics and biological and physical properties of viruses, the issues of combating them are very acute. Microorganisms resistant to many antimicrobial agents are also developing (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, etc.). Th erefore, today there is an urgent need for new materials with a higher antiviral and bactericidal eff ect, less toxicity for humans and the environment (ecology, medicine and food industry). Silver-containing biomaterials in various forms (powders, fi lms, solutions, products formed by 3D printing technology) with eff ective antimicrobial and antiviral eff ects have been developed. Th e materials are made on the basis of polyelectrolyte complexes of polysaccharides (pectin – chitosan, pectin – cationic starch, carboxymethylcellulose – cationic β-cyclodextrin, anionic starch – cationic starch) and polylactide biopolymer. Silver nanoparticles in the composition of composites were formed in environmentally friendly ways: by reduction silver ions with extracts of natural substances (green tea, mint, propolis, pectin), by methods of thermochemical reduction or cathodic sputtering of silver nanoparticles from silver foil onto the surface of the fi lm. Th e structure, morphology, obtained materials and their antimicrobial, antiviral and cytotoxic properties were studied. Th e relationship between the structure and morphology and the protective properties of the obtained materials was studied. [ABSTRACT FROM AUTHOR]