Search

Introduction: Diabetic foot ulcer is a complication of diabetes mellitus and can be associated with an increased risk of amputation or even death as a result of a wound infection. E.coli can lead to worsening of the condition of the wound. The aim of this study was to identify β-lactamase genes in E. coli isolated from diabetic foot ulcers Materials and Methods: In this experimental study, 110 isolates of E. coli from patients with diabetic foot ulcers hospitalized in the infectious ward of the in Peymanieh, Jahrom and Ali Asghar and Namazi, Shiraz, hospitals in 1396 were selected. Determination of antibiotic susceptibility, phenotypic identification of strains producing broad-spectrum beta-lactamases (ESBLs), strains producing MBL and strains producing Carbapenemas was done. The existence of genes encoding bla TEM, bla SHV and bla OXA-1 enzymes was investigated by multiplex PCR assay Results: In this study, 35 isolates of Escherichia coli were isolated. 13 isolates produced broad-spectrum betalactamases. Genetically, 19 isolates with TEM gene (54.2%) and 3 isolates with SHV (8.5%), which simultaneously had 1 isolate from 3 isolates with SHV, also had OXA-1 gene (2.8%). The highest susceptibility of Escherichia coli was to maropenem (99%) and the lowest antibiotic susceptibility to amikacin (3.2%). Conclusion: According to the results of this study and monitoring of antibiotic resistance in patients with diabetic foot ulcers, treatment of infections caused by this agent can be facilitated. [ABSTRACT FROM AUTHOR]

Introduction: Orchid roots are commonly used in traditional medicine as an energizing drug and sexual enhancer. The present study was conducted to investigate the effect of the aqueous extract of Orchid roots on the serum concentrations of progesterone and luteinizing hormones in female rats. Materials and Methods: The present experimental study was conducted on 40 adult female Wistar rats randomly divided into 5 groups of equal sizes, including the negative control group (receiving no drugs), the sham control group (receiving 1 ml of distilled water) and experimental groups 1, 2 and 3 (receiving 20, 40 and 80 mg/kg aqueous extract of Orchid roots, in respective order). The extract was injected intraperitoneally to the experimental groups for 28 days. At the end of the experimental period, blood samples were taken from the rats to examine their serum levels of progesterone and LH (p<0.05). Results: Injecting the aqueous extract of Orchid roots to the experimental groups at 40 and 80 mg/kg doses increased the serum levels of progesterone and LH significantly compared to in the negative and sham control groups (p <0.05). Conclusion: The aqueous extract of Orchid roots can have a positive effect on ovulation in females through increasing their serum concentrations of progesterone and LH. [ABSTRACT FROM AUTHOR]

Introduction: The growing prevalence of obesity is one of the biggest health challenges across the world. Stimulating the body metabolism and increasing its energy use through herbal agents is one of the main methods used to treat obesity. Materials and Methods: This study was conducted on 50 adult male Wistar rats weighing between 180 and 200 g randomly divided into 5 groups of equal sizes, including a negative control group (receiving no drugs), a sham control group (receiving 1 ml of distilled water) and experimental groups 1, 2 and 3 (receiving 20, 40 and 80 mg/kg aqueous extract of Orchid roots, in respective order). The extract was injected intraperitoneally to the experimental groups for 28 days. On the 29th day, the rats' blood samples were taken to examine their serum concentration of cholecystokinin. The rats' body weight was measured on a daily basis throughout the experiment. Statistical analysis were performed in SPSS using the one way ANOVA and Duncan's post-hoc test. Results: Orchid root aqueous extract injection in a dose-dependent led to significant increase in serum levels of cholecystokinin hormone and decrease in food intake and body weight than the control group (p<0.05). Conclusion: Through increased serum levels of cholecystokinin hormone, aqueous extract of orchid plant root reduces body weight. [ABSTRACT FROM AUTHOR]