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【目的】用RT-PCR 法获得人类神经元性芳香族氨基酸脱羧酶(AADC)基因全序列, 并建立优化方法。【方法】 从人嗜铬细胞瘤中提取组织总RNA , 自行设计引物, 采用两步RT-PCR 法扩增出长约1 550 bp 的基因片断, 凝胶回收后与高 效克隆PCR 产物的新型载体T-载体连接, 并转化入大肠杆菌, 通过蓝白斑反应筛选出重组体, 质粒提取后进行重组体的酶切 鉴定及基因测序。【结果】BamH Ⅰ 和Hin dⅢ 酶切证实了目的基因同克隆载体进行了有效的连接, 基因测序证实了所克隆的 片断为人AADC 基因。【结论】本实验通过对引物, Taq 酶的选择及反应条件的优化, 保证了扩增的有效性和特异性。新型 的克隆载体明显提高了克隆效率。基因测序证实了AADC 基因扩增的成功。本研究为AADC 基因治疗帕金森病等多巴胺能 缺乏疾病的研究打下了基础。

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The ore (rock) bodies between the 570 m and 630 m levels in Jiaojia Gold Mine are characterized by well-developed joints and fissures, prone to forming wedge-shaped unstable structures, leading to rock mass col- lapse. Geological drilling and structural surface condition surveys indicate that the rock mass in Jiaojia Gold Mine is relatively fractured, with well-developed joints and a wide range of joint occurrence distributions. This study investi- gates and summarizes the convergence deformation patterns of roadways under mining disturbances. Radar testing and analysis reveal that the damage depth of the roadways in Jiaojia Gold Mine generally remains at about 2 m, with many weak structural surfaces present, necessitating timely reinforcement measures. This research provides a rational plan for the support of the ore body and guides the reasonable selection of stope structural parameters to ensure safe production in this mining section. It offers valuable insights for managing fractured rock mass in similar mines. [ABSTRACT FROM AUTHOR]

The cast-in -place wave wall of the west artificial island of the Shenzhen -Zhongshan Link adopts a ladder-like structure with complex section forms. The structural foundation is replaced with filling foundation and there are differences along the cross section direction. Based on the considerations such as controlling differential settlement of the wave walls, improving construction quality of wave walls, and enhancing architectural landscape effects, an optimization research was carried out on the structural design and construction technology of cast-in-place wave walls. The practice has proved that the optimized structural design is scientific and reliable, and the construction process is safe and efficient. The structure of the wave wall after implementation has no obvious difference settlement, the structure line is smooth, and the landscape effect is good, which verifies the scientific and feasibility of this optimization scheme, and can provide relevant reference for similar projects. [ABSTRACT FROM AUTHOR]

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Based on the data of Kenya's four national population censuses, exploratory spatial data analysis, standard deviation ellipse, and population shift-share model were used to analyze the spatial pattern evolution characteristics of the population distribution of 47 administrative units in Kenya from 1989 to 2019. Then, this paper employed geographic detector to further analyze the influencing factors. The results show that: ① During the study period, the distribution pattern of population density in Kenya is relatively stable, showing high southwest and low northeast. ② There are obvious spatial differences in population offset growth in different cities, mainly positive offset growth and negative offset growth types. And the population distribution center is northwest of the capital Nairobi and continues to migrate southeast. ③ Natural and socio-economic factors jointly influence the spatial evolution pattern of population distribution in Kenya. Under the influence of elevation, climate, precipitation, economic development level and transportation infrastructure construction, the population is concentrated in a few areas along the southwest and southeast coasts of Kenya. Kenya has roughly formed a high-density population cluster centered on Nairobi, the national administrative center, Kisumu on the shores of Lake Victoria, and Mombasa, the largest port on the coast. [ABSTRACT FROM AUTHOR]

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Science and technology is the main support of modern agriculture. To start a new journey of Chinese agricultural modernization, we must always adhere to the principle that agricultural science and technology are the first productive force, education and technical training are the premise of promoting agriculture and strengthening agriculture, and talents are the first resource, so as to build an integrated system of highly integrated agricultural education, science and technology, and talents. Aiming at the main problems existing in the process of high quality agricultural development, this paper puts forward targeted policy suggestions on building an integrated system from three aspects: overall integration of agricultural training competent departments and institutions, strengthening agricultural science and technology promotion, and vigorously implementing the “three strategies”, so as to provide basic and strategic support for promoting high-quality agricultural development and building agricultural modernization. [ABSTRACT FROM AUTHOR]

Upper- and lower-flow regimes are formed under different conditions. To identify them is important for predicting mobile bed resistance. Based on the dimensionless resistance equation, a new discriminant function to flow regimes is deduced, which contains two dimensionless parameters, namely, the suspension number and the flow action number. The concepts of the flow regime transition angle and the flow regime transition path are proposed. The problem of the discriminant flow regime is revisited from the perspective of flow resistance. The discriminant function is calibrated by 4 176 sets of flume and field measurement data at home and abroad, and the critical equation between the upper-and lower-flow regimes is determined. The results show that the prediction accuracy of the proposed critical equation is approximately 91% in the lower regime and 85% in the upper regime, and the comprehensive accuracy is approximately 89%. In fine-sand rivers, when the water temperature decreases, the transition from the lower-flow regime to the upper-flow regime is more likely to occur. The discriminant function proposed in this study can not only identify the flow regime with high accuracy but also analyze the effect of water temperature on the flow regime transition. [ABSTRACT FROM AUTHOR]

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For the problem of group evaluation in complex uncertain environments, an interval rough number is used to characterize experts’ preferences, and a stochastic simulation integrated algorithm is proposed based on the G1 method combined with Monte Carlo simulation techniques. Firstly, the weighting coefficients are simulated by random sampling and the weighting of experts are determined based on ordinal correlation and interval rough number closeness. Secondly, the final weights of indicators can be obtained by combining all the experts’ opinions, and they are linearly aggregated with the pre-processed indicator values to obtain the comprehensive evaluation value of one simulation, which can be used to assess the advantages and disadvantages of the evaluated objects. Through full simulation, the preference ratio matrix is calculated, and the probability ranking with the probability of superiority is derived from it, which makes up for the deficiency of absolute ranking in the uncertain information environment. Finally, the effectiveness of the method is illustrated by an example and the advantages of the method are described in comparison with the existing methods. [ABSTRACT FROM AUTHOR]

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In the past decade, thyroid surgery in China has developed rapidly. With the improvement of thyroid diagnosis technology, the standardization of thyroid surgery, the clinical application of systematic therapy, and the progress in fundamental research, thyroid surgery has achieved all-round development. Patients with thyroid disease have benefited significantly from safer, more standardized, more effective, and more diverse treatments, which results in the remarkable increase in the 5-year survival rate. Moreover, Chinese experts have published a number of guidelines and expert consensus related to thyroid surgery, which promotes the standardization of the diagnosis and treatment of thyroid disease. Additionally, the specialized multidisciplinary teams in cancer centers allow patients to receive more standardized diagnosis and treatment of complex thyroid diseases. In the present article, we summarize the important developments of China's thyroid surgery in the past decade, including preoperative diagnosis technology, surgical methods, operation safety, application of minimally invasive techniques, diagnosis and treatment of special thyroid cancers, multidisciplinary diagnosis and treatment models, and standardized postoperative managements. We expect to popularize the concept of standardized diagnosis and treatment of thyroid diseases, and improve the level of standardized diagnosis and treatment in thyroid surgery. [ABSTRACT FROM AUTHOR]

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Sub lacustrine fan on the background of fault slope development in the lowstand system tract of the third member of the Shahejie Formation, Laizhouwan sag. It has a clear seismic reflection geometric appearance, but the internal structure of the sub lacustrine fan is chaotic, the vertical stacking relationship and plane enrichment position of the sand body were difficult to predict in the exploration and early evaluation stages. Based on drilling,logging,laboratory date,and three-dimensional seismic data, the principles of rheological properties and fluid concentration classification of gravity flow channels in sub lacustrine fan were applied, the sedimentary transport forms of gravity flow were divided into sliding, collapse, debris flow and turbidity flow. Based on the seismic reflection characteristics of channel sand bodies with different genetic transport mechanisms, five sedimentary structural units were identified and characterized in different facies zones of the sub lacustrine fan,they were stacked channels, composite channels, channel wings, single channels, and inter-channels,in addition, the reliability of different unit classification and the differences in sand content through model forward modeling was verifiem. The results indicate that the superimposed channel of the inner fan has developed multi-stage sliding collapse sedimentation, and the overall thickness of the reservoir is large. The composite channel in the lower part of the middle fan has developed sandy debris flow reservoirs with good physical properties, both of which are favorable locations for oil and gas exploration and drilling. The development level of the outer fan reservoir is relatively low. The highly constructive braided river delta from the Kendong uplift provides a large amount of sandy foundation for the sub lacustrine fan, and the slope break zone and ancient landforms jointly control the sedimentary transport mechanism, channel direction, and channel type of the sub lacustrine fan. [ABSTRACT FROM AUTHOR]

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