Search

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To study the influence and Novel Function mechanism of micro RNA-195 in the process of Glucose Uptake Disorder Induced By PA in Human Liver Cell L02. Methods: Human Liver Cell L02 was cultured in vitro and treated with different concentrations of PA(0.6 mmol/L, 1 mmol/L) for 10 h.The morphological changes of Human Liver Cell L02 were detected by microscope. GOD-POD detect the rest glucose content in cell culture, the expression levels of miR-195 was analyzed by Real time-PCR after induced by PA. Measured fluorescence intensity by Laser confocal scanning microscopy, and the change of glucose uptake by up-regulated and down-regulated of miR-195.Results: High concentration PA could induced Liver Cell L02 Glucose Uptake Disorder, the glucose content increased 2.5 mmol/L, and miR-195 over-expressioned.After transfection of miR-195 mimics, the expression of miR-195 up-regulated, and transfection of miR-195 inhibitor the expression of miR-195 down regulated. The concentration of glucose in cell culture was increased 2.5 mmol/L when miR-195 over-expressioned, that is to say glucose uptake reduced.The concentration of glucose decreased 1mmol/L in cell culture decreased when miR-195 low-expressioned. Conclusions: miR-195 is very likely to regulate the disorder of glucose uptaken in. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the effects and existing problems of PBL implemented on the Nutrition and Food Hygiene teaching, and to evaluate the results of PBL. Methods: To divide the students into the PBL teaching group (students of five-year program Nutriology, 2012 year) and the traditional teaching group (students of five-year program clinical medicine, 2011 year) randomly, and to evaluate the teaching effects by the examinational test and the questionnaire survey (including three questionnaires: student's satisfaction questionnaire, the way of problem-resolving questionnaire and a learning interesting/motivation questionnaire). Results: Compared with traditional teaching pattern, PBL teaching pattern actually has better effects on improving test scores, student satisfaction index, the learning interesting/motivation, and problem-solving way. Conclusion: PBL is a successful teaching method for five-years' program military students which could improve the teaching quality and enhance the capabilities of students, as well as strengthen the intrests of learning, that is worth of further promoted. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To study the influence of Eicosa Pentaenoic Acid(EPA) on the apoptotic, the regulation of h TERT and telomerase activity of human hepatocarcinoma SMMC-7721 cell. Methods: Human hepatocarcinoma SMMC-7721 cell was cultured in vitro and treated with different concentrations of EPA(0 μM, 25 μM, 50 μM, 100 μM, 200 μM) for 24 h, 48 h, 72 h. The morphological changes of SMMC-7721 hepatoma cell were detected by microscope. MTT assay was explored to detect the proliferation of SMMC-7721cells; The expression levels of h TERT, Bcl-2 and Bax protein were detected by Western blotting; The expression levels of SMMC-7721 cell h TERT m RNA were analyzed by Real time-PCR; ELISA was used to detect SMMC-7721 cell telomerase activity levels. Results:EPA induced SMMC-7721 cells apoptosis significantly, and the apoptosis rate was both in time-and dose-dependent manner. Western blotting showed that expression levels of h TERT and Bcl-2 protein was down-regulated and expression levels of Bax protein was up-regulated concurrently along with the apoptotic process, and the expression of h TERT m RNA as well as activity of telomerase decreased concurrently. Conclusions: EPA can restrain the activity of telomerase and induce death of SMMC-7721 cancer cells by inhibiting the expression of h TERT m RNA, it may be one of the anti-cancer mechanisms of EPA. [ABSTRACT FROM AUTHOR]