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The research block is characterized by extensive development of low-resistance oil formations, with little difference in resistivity between the oil and water formations, making logging fluid identification more difficult. In order to effectively identify the oil layer of low resistivity, synthetic minority oversampling technique(Smote) was adopted to oversample a few types of samples such as oil-water homogeneous layer, oil layer, etc. to equalize the dataset, and the gated recurrent unit (GRU) network model was utilized for fluid identification in low resistance reservoirs. Eight logging curve data such as natural gamma-ray (GR), deep resistivity(RD),density(DEN) were identified as input training models through correlation analysis and applied to the medium actual data, and GRU was compared with traditional recurrent neural network(RNN) and other three machine learning algorithms. The results show that the sequential data model is better than the traditional machine learning model for fluid recognition, and interpretation agreement rate of the Smote-GRU-based fluid recognition model reaches 89. 5%,compared to 81. 1% of traditional RNN, which achieves better application results. It is also confirmed through controlled experiments that the Smote algorithm improves the recognition rate of the classifier for minority class samples. The proposed method can provide a reference for fluid identification in oil layers of low resistivity with sample imbalance [ABSTRACT FROM AUTHOR]

Objective To evaluate the effect of Chinese botulinum toxin type A on gastrocnemius hypertrophy by B-mode ultrasound combined with calf circumference measurement. Methods From August 2020 to October 2021, twenty-one female who were diagnosed as gastrocnemius hypertrophy were enrolled in this study. All the patients were treated with botulinum toxin type A at Plastic Surgery Hospital of Peking Union Medical College, CAMS. Twenty-one female were enrolled in this study. Each side of gastrocnemius muscle was injected with 100 U each case. Calf circumference of the thickest part of both legs was measured before and at 4, 12, and 24 weeks after injection and the thickness of the medial and lateral head of bilateral gastrocnemius muscles at contracted status was measured preoperatively, 12 weeks and 24 weeks postoperatively using B-ultrasound. Results The 21 patients were followed up for 6 months. The thickness of gastrocnemius muscles is (14.06±2.41) mm (the medial head) and (12.46±1.43) mm (the lateral head) at 12 weeks and the mean reduction of gastrocnemius muscles thickness were (19.44±11.46)% in the medial head and (25.71±8.57)% in the lateral head. At 24 weeks, the thickness of the muscle has a slight growth compared to 12 weeks, which is (16.46±1.78) mm (the medial head) and (15.86±1.14) mm (the lateral head). The reduction in calf circumference was (0.98±0.8)% at 4 weeks, (3.86±1.55)% at 12 weeks, and (1.63±0.94)% at 24 weeks. Conclusion Botulinum toxin type A is effective in treating hypertrophy of the gastrocnemius muscle. B-ultrasound combined with calf circumference measurement could effectively reflect the reduction of muscle thickness after domestic botulinum toxin type A injection. [ABSTRACT FROM AUTHOR]

Rice (Orysa sativa L.) is one of the most important food crops in China, playing a major role in guaranteeing food security. Thanks to the development of science and technology, especially the progress of rice breeding technology, there have been two rounds of substantial increase in China’s rice yield since the large-scale application of dwarf rice breeding and hybrid rice technology. Consumer demand for rice varieties and rice products is becoming increasingly diversified in the new era, and the current efficiency of rice breeding is still not satisfactory enough to meet future demand. As a self-pollinated crop, rice requires multiple generations of continuous self-crossing to achieve genetic homozygosis and stability in segregating generations during the breeding process. However, the long growth cycle of rice in paddy field and the difficulty of year-round planting have led to a long breeding cycle, which has become one of the crucial bottlenecks in rice breeding. Speed breeding is a new technology rising in recent years that promotes crop growth, flowering and maturation through comprehensive measures such as environmental regulation and growth manipulation to expedite the crop generation advance, which can shorten the “seed to seed” cycle and realize multi-generation breeding annually, and promises to significantly shorten the breeding cycle and improve the breeding efficiency. In this review, we focus on the rice crop and systematically describe the research status and progresses of speed breeding in rice in terms of the rise of crop speed breeding technology, the research overview of rice speed breeding, key genes in rice speed breeding, environmental factors affecting the rice flowering and maturation, and the incorporation of speed breeding and modern breeding technology. Further, we discuss and look forward to the challenges and key directions of future research in this field, aiming to promote the rapid development and application of speed breeding technology in rice, improve the efficiency of rice breeding, and provide useful references for guaranteeing food security and sustainable development of agriculture. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of high uric acid on cardiomyocyte activity in vitro and its related mechanism. Methods H9C2 cells were divided into the control group (Con), the high uric acid group (HUA), the antioxidant group (NAC), the ERK inhibitor group (PD98059), the P38 inhibitor group (SB203580), the high uric acid + antioxidant group (NAC+HUA), the high uric acid + ERK inhibitor group (PD98059+HUA), the high uric acid + P38 inhibitor group (SB203580+HUA) and the high uric acid + antioxidant + ERK inhibitor group (NAC+PD98059+HUA). MTT method was used to detect the vitality of cardiomyocytes. Immunofluorescence staining and flow cytometry were used to detect the reactive oxygen species (ROS). Western blot assay was used to detect the protein expression levels of extracellular signal regulating kinase (ERK) and phosphorylated P38. Results After treatment with high uric acid (0.15 g/L), the activity of cardiomyocytes decreased significantly. Compared with the Con group, the oxidative pressure increased significantly in the HUA group, while compared with the HUA group, the oxidative pressure decreased in the NAC+HUA group (P＜0.05). Compared with Con group, the activity level of cardiomyocytes decreased significantly in the HUA group, while compared with the HUA group, the activity levels of cardiomyocytes increased significantly in the NAC+HUA group, PD98059+HUA group and SB203580+HUA group (P＜0.05). Compared with the Con group, phosphorylation levels of ERK and P38 were increased significantly in HUA group. Compared with the HUA group, the phosphorylation levels of P38 of SB203580 group were decreased significantly, while the phosphorylation levels of ERK and P38 were decreased significantly in NAC+HUA group, PD98059+HUA group and NAC+PD98059+HUA group (P＜0.05). Conclusion High uric acid inhibits myocardial cell activity by activating ERK/P38 signaling pathway through oxidative stress in vitro. [ABSTRACT FROM AUTHOR]

Ecologically fragile regions such as loess hilly-gully region are highly responsive to global change. Regional development is on an unsustainable track under the background of fragile ecological environment and agricultural activities. The study of ecological risk in this region has become one of the hot spots of geographical and ecological response to ecosystem management. Taking Mizhi county for the study of loess hilly-gully region, land-use maps of this county in 2009 and 2015, a digital elevation model (DEM) and social and economic statistical yearbook of Mizhi county were needed and 253 small watersheds were identified as the auxiliary evaluation units by using ESRI's Hydrological Analysis Tools for ArcGIS 10.2. A comprehensive ecological risk assessment framework for identifying risk probability, habitat sensitivity, and system impairment was established, and multi- source data were integrated through range standardization and entropy weight method at watershed level. The spatiotemporal differentiation of criteria layers and comprehensive ecological risk were analyzed, at the same time, the evolution trend of comprehensive ecological risk was explored through the Standard Deviation Ellipse (SDE) and risk gravity. The risk precaution partitions were simultaneously delineated based on the risk dominant factor and the risk management strategies were formulated. The results were as follows: (1) The spatial distribution pattern of risk probability in Mizhi county was generally high in the middle and east and low in the northwest. The value of risk probability increased from 49.93% to 52.92% during 2009-2015. The habitat sensitivity index was 0.61 in 2009 and 0.60 and 2015, respectively, which was a decrease of 1.6% based on the InVEST model, and the habitat quality improved. The system impairment index was generally higher in the central and western regions while lower in the eastern region (southeast and northeast). The impairment index increased from 0.42 to 0.46 during 2009-2015, which indicated that the degree of impairment caused by ecological risk increased. (2) The spatial distribution pattern of comprehensive ecological risk was high in the middle, while low in the south and north. Between 2009 and 2015, the ecological risk index increased from 0.14 to 0.15, with an increase of 7.14%. The risk gravity of Mizhi county was distributed in Gaoqu township in 2009, and jumped into Yinzhou township in 2015. The main ecological risk evolution was northwest-southeast trending, and kept stable basically. (3) The study area was divided into four types of risk precaution partitions. Risk monitoring zones, ecological recovery zones, monitoring and recovery zones, and natural regulation zones encompassed 7.53%, 6.57%, 23.86% and 62.04% of the study area, respectively. Risk precaution partitions based on risk dominant factors can effectively eliminate risks and promote the sustainable development of regional habitats. [ABSTRACT FROM AUTHOR]

2'-fucosyllactose（2'-FL）is the most abundant human milk oligosaccharide. Microbial fermentation is an important method to produce 2'-FL. Lactococcus lactis（L. lactis）, a food safety grade strain, can be directly used in dairy products, has not been reported for the production of human milk oligosaccharides. First, the 2'-FL salvage synthesis pathway was constructed by using a recombinant plasmid pNZ8148-2f containing gene fkp, futC, lacF in two usual L. lactis chassis NZ3900 and NZ9000. In the fermentation medium with 10 g/L fucose and 5 g/L lactose, the yields of 2'-FL in flasks were 0.16 g/L and 0.4 g/L, respectively. Combined with the growth assay, NZ9000 was considered as a better chassis. Second, 2'-FL de novo synthesis pathway was constructed in NZ9000, i.e., using homologous recombination technology and the unique knockout integration vector pNZ5319 in L. lactis，integrating the gene manA, manB, gmd and wcaG to the chromosome while knocking out unnecessary gene upp, and introducing the gene manC, futC and lacF to the cell by vector pNZ8148-1. Meanwhile, different promoter P32 and Pnis were used to optimize the expressions of the pathway enzymes. The best strain NZ9000 6 was obtained with a 2'-FL production of 0.28 g/L in flask fermentation medium with 20 g/L glucose and 5 g/L lactose. Finally, the concentrations of hemin, tryptone and yeast extract were optimized in the fermentation medium. With the addition of 2.5 μg/mL methemoferin chloride, 15 g/L tryptone, and 6 g/L yeast extract in the fermentation medium, the final 2'-FL production reached 1.58 g/L which was relatively high among the reported results. Thus, the feasibility of 2'-FL synthesis by L. lactis was proved, which provides a new idea for the production of 2'-FL. [ABSTRACT FROM AUTHOR]

Compared to the rest of the world, Asia shows greater differences in socioeconomic and political systems among its countries. Such differences are not conducive to regional integration. It is my view that the integration of the world is a desirable goal and is inevitable. Of course, such a process takes a long time and Asia is no exception. In order to realize the integration of Asia, I argue that Asia needs to achieve what I call Asianization, a process that will remove ideologies (de-ideology), facilitate communication, and promote shared value within Asia. All these will lead to the integration of Asia. Finally, I propose that the East Asian region build a “Network of West Pacific Metropolitan Areas” to institutionalize the exchange within Asia. [ABSTRACT FROM AUTHOR]

To explore the differences between parallelogram incision and fusiformis incision in the harvest of hair follicular unit. Methods From October 2017 to January 2020, the hair follicles in 127 patients were harvested by parallelogram incision or fusiformis incision. The number of hair follicles in 1 cm2 scalp stripe were calculated. The differences of follicular unit acquirement rate between parallelogram incision and fusiform incision were compared by paired t-test. Results The hair follicle acquirement rate by parallelogram incision was significant higher than that by fusiformis incision. The difference was statistically significant (P<0.05). Conclusion In the harvest of hair follicles by scalp stripe method, parallelogram incision could achieve more hair follicles and less damages than fusiformis incision. [ABSTRACT FROM AUTHOR]

With the integrated development of the Yangtze River Delta as a national strategy, urban development in the Nanjing metropolitan area has entered a new stage. However, facing the large differences between the newly joined districts and counties are the key issues of ensuring the driving role of Nanjing as a central city and guiding future high-quality development of the metropolitan area by planning the industrial layout. In this study, enterprise big data and impervious surface data were used as the sources to employ fractal theory, GIS spatial analysis, and other techniques to study the historical evolution characteristics of the built-up areas in the Nanjing metropolitan area. The spatial pattern changes of their expansion intensity have gone through three periods: 1990-2000, 2000-2010, and 2010-2017. The following stages were observed: 1) In the first stage, the expansion intensity varied widely across the area with the overall pattern being unclear to date. 2) In the second stage, a circular structure emerged gradually with high-value cores located in the main downtown area of Nanjing and the intensity of expansion decreasing from the core to periphery. 3) In the third stage, the number of cores within the circle gradually increased to include Chuzhou Langya District, Huaian Qingjiangpu District, and the border area between Zhenjiang and Changzhou. Based on these stages, the spatial correlation between industrial agglomeration and spatial evolution of built-up areas in the Nanjing metropolitan circle were further analyzed using quantitative indicators such as the Moran index and locational entropy. The following results were obtained. 1) A global positive correlation was observed between the agglomeration degree of manufacturing industries in the metropolitan circle and the spatial evolution intensity of built-up areas during 1990-2015; however, this global-level correlation has decreased in recent years owing to the slower development of manufacturing industries in some regions than the expansion of built-up areas. 2) The structural differences between the manufacturing industries in each region directly influenced the intensity and speed of built-up area expansion. Additionally, different types of manufacturing transfers caused differences in the speed and intensity of expansion of built-up areas in the hinterland of metropolitan circles, affected by the general trend of industrial division of labor in metropolitan circles. 3) The mineral processing industry showed the widest spread among all laborintensive and low value-added industries. It also possessed the widest diffusion range and played a driving role in the development of built-up areas in each district and county in the long term, whereas for the remaining industries, the initially strong development of built-up areas weakened thereafter. Therefore, it could be argued that the Nanjing metropolitan circle is in a critical period of coordinated regional development, and the initiatives to transfer low-end manufacturing industries to other regions in the circle must be taken in Nanjing while continuously improving its own supremacy by means of collaborative development and innovation. Simultaneously, the sloppy land resource investments in other regions must be altered to improve efficiency. [ABSTRACT FROM AUTHOR]

目的探讨趋化因子生长调节基因5(CXCL5)及其受体CXC类趋化因子受体2(CXCR2)在非小细胞肺 癌（NSCLC)发生、发展中的作用。方法选取86例NSCLC患者的手术切除肿瘤组织及其癌旁正常组织标本，采用 免疫组化法检测CXCL5、CXCR2表达。分析CXCL5、CXCR2表达与肿瘤分化程度、临床分期、血管浸润的关系。 结果NSCLC组织CXCL5、CXCR2阳性、强阳性率均高于癌旁正常组织（P均<0.05)。CXCL5、CXCR2表达与肿瘤 组织分化程度、临床分期、血管浸润均有关（P均<0.05)。结论NSCLC组织CXCL5、CXCR2高表达。CXCL5、 CXCR2高表达可促进NSCLC的发生及发展。。 [ABSTRACT FROM AUTHOR]

目的探讨CTTIVl基因沉默对非小细胞肺癌A549细胞侵袭能力的影响。方法将非小细胞肺癌 A549细胞随机分为观察组与对照组，两组均采用Upofectamine 2000脂质体转染法分别转染CTTN小干扰RNA (siRNA)、阴性siRNA。作用48 h，采用Western blotting法检测两组皮层肌动蛋白（Cortactin)相对表达最；以红色 荧光Trite-鬼笔环肽标记的F-actin和绿色荧光Dyligt488标记的Cortactin共定位黄色荧光的侵袭性伪足，采用激 光共聚焦M微镜观察并计算侵袭性伪足百分率;采用Transwell小室试验检测两组细胞侵袭能力（以穿过基底膜 的细胞数表示>。结果观察组与对照组Cortactin相对表达毋分别为0. 17 ±0.02、0.79 ±0. 15,侵袭性伪足百分 率分别为（22.37±4.8)%、(71.46±8.9)%，穿过基底膜的细胞数分别为（51±10)、（220±27)个，两组比较P 均<0.05。结论沉默CTTN基因可通过减少非小细胞肺癌A549细胞侵袭性伪足的产生而降低其细胞侵袭 能力。 [ABSTRACT FROM AUTHOR]

In order to solve the problem of mass concrete cracks in the cast-in-situ buried section of the Shenzhen-Zhongshan Link tunnel, the application research of anti-cracking technology is carried out from the aspects of materials to construction technology. In terms of materials, strict control of raw materials and optimization of concrete mix ratio are adopted, and half section pouring method is adopted in construction technology. The construction reduces external constraints, and is supplemented by measures such as temperature control of concrete entering the mold, laying cooling circulating water pipes, and strengthening surface heat preservation, moisture conservation and maintenance. On the basis, anti-cracking technology scheme is formulated to better the crack problem of mass concrete in the cast-in-place buried section. Based on the actual monitoring data, the supporting application technology of hydration heat control materials is proposed, which further ensures the anti-cracking requirements of concrete and provides ideas for similar projects. [ABSTRACT FROM AUTHOR]

[Objective] Field investigation and sampling of the soil and kidney beans in the farmland beside the Songma section of Kunming Highway X013, to determine and analyze the content of Pb, As and Cu in the soil and kidney beans. [Method] Using the comprehensive pollution index method The heavy metal pollution in the soil was evaluated. [Result] At a distance of 5 m from the road, the Pb-excess rate of soil and kidney beans were both 100%, and both were polluted by Pb; at a distance of 20 m from the road, the soil pollution level reached the warning line. Slightly polluted. At a distance of 50 m from the road, the soil and string beans are not polluted by Pb and As, but the soil is seriously polluted by Cu, showing a trend of decreasing with the increase of the distance from the road. Cu pollution. [Conclusion] Pb in green beans is mainly caused by automobile exhaust emissions. It is recommended that green beans be planted 50 m away from the road. The content of As in green beans is very small and is not affected by changes in distance from the road. Soil The Cu pollution is serious, but the kidney beans are not contaminated by Cu, indicating that the soil Cu has not migrated to the edible part of the kidney beans. [ABSTRACT FROM AUTHOR]

Drawing upon the high-quality resources of the virtual teaching and research platform for organic chemistry, this study delves into the construction of an organic chemistry knowledge graph. Knowledge units and points shape the hierarchical "nodes", while the "relations" between these "nodes" weave a network that intimately integrates the course content. Collaboratively developed teaching resources are embedded into the knowledge graph, offering comprehensive support for talent development and pedagogical activities in organic chemistry. [ABSTRACT FROM AUTHOR]

In order to overcome the influence of shallow seas, low headroom and existing bridge structure on the construction of jet grouting piles, the construction technology of mobile working platform + jet grouting with connecting rods at sea was studied and put into practical application, based on the contraction practice of the East Artificial Island of Shenzhen-Zhongshan Link. Engineering practice shows that: 1) the construction technology of mobile working platform + jet grouting with connecting rods effectively solves the technical problems in jet grouting pile construction at sea and the working platform has a wider applicable range; 2) the dynamic speed control system based on theoretical calculation formulae can better ensure the quality of jet grouting pile construction, and the deviation rate of cement dosage is only 2.4% ; 3) the impact of the construction process on the existing bridge structure is mainly manifested in horizontal displacement in the down-bridge direction and the deformation value fluctuates in the range of -1.3 ~1.7 mm, which meets the high requirements for deformation control of extremely sensitive buildings (structures). [ABSTRACT FROM AUTHOR]

Objective: To investigate the method of inducing mice SVF cells derived from white fat into beige fat cells, and to provide a cell model for the study of beige fat. Methods: SVF cells derived from mouse adipose tissue were isolated and cultured, and their cell morphology and growth characteristics were observed. The cells were treated with cocktail method and beige fat induction method, respectively, and cell samples were collected at the 0, 2, 4, 6, 8 and 10 days of induction for Western blot and RT-qPCR. Lipid production in the petri dishes at different time points was observed by oil red O staining, and lipid drop size was quantified. Results: The isolated SVF cells showed a long spindle shape gradually, and a spiral growth trend after 120 h inoculation. Western blot and RT-qPCR results indicated that Peroxisome proliferator activated receptor γ (PPARγ) could be induced by both methods. The gene expressions of PPARγ and CCAAT/enhancer binding proteins α (C/EBP-α) were increased (P＜0.05) . Oil red O staining showed no difference in lipid production levels under the induction of the two methods, but compared with that in the cocktail method, Fat droplet area of fat cells induced by beige fat decreased significantly (P＜0.05), and the percentage of small fat droplet was higher. Western blot and RT-qPCR results showed that compared with that by the cocktail method, the beige fat induction method enabled the uncoupling protein 1 (UCP1) and an increased gene expression of PRD1-BF1-RIZ1 homologous domain containing 16 (PRDM16) (P＜0.05). Conclusions: Compared with the cocktail method, this method can induce SVF cells to differentiate into beige adipocytes successfully. The adipocytes have the characteristics of beige adipocytes and express the beige adipocyte marker genes. [ABSTRACT FROM AUTHOR]

【Objective】Tolerance to hypoxia germination is one of the key traits for direct-seeding adaptability of rice. The temperate direct-seeding japonica rice variety Francis and multi-panicle indica restorer line R998 were used for QTL mapping of hypoxia germination related traits, aiming to provide new valuable gene resources for developing direct-seeding rice varieties and the promotion of direct-seeding rice production methods.【Method】The coleoptile length, shoot length and maximum root length of rice seedlings submerged in 10-cm-depth water in the dark for 7 days at 28℃ were recorded as indicators of hypoxia germination tolerance capacity. A high-density genetic map containing 3 106 bin markers was constructed by low-coverage genome re-sequencing, and QTL scanning was performed with WinQTL Cart 2.5.【Result】Under the condition of hypoxia germination, the coleoptile length and root length of Francis are significantly higher than those of R998, but there is no significant difference in shoot length between them. The total genetic distance of the constructed genetic map is 3 646.2 cM, in which the number of markers on chromosome 12 is the least, and that on chromosome 1 is the most, with 174 and 389, respectively. The average marker distance in the genetic map is 1.21 cM, the marker distance of each chromosomes is 0.68 -1.84 cM, and the gaps above 5 cM account for 0.36%. By using composite interval mapping (CIM), six QTLs related to hypoxia germination tolerance are detected on 5 chromosomes. Among them, the number of QTLs controlling coleoptile length, shoot length and root length are 3, 1 and 2, respectively. The coleoptile locus qCL9 and the shoot length locus qSL5 had a phenotypic variance explained (PVE) over 10%, which are 13.39% and 10.78%, respectively. The beneficial allele of qCL9 and the root length locus qRL2-1 come from the parent Francis, while the other four beneficial alleles are provided by the parent R998. Three of the six QTLs have not been reported previously, therefore, they may be novel QTLs.【Conclusion】In summary, there is significant difference between Francis and R998 in hypoxia germination, and both parents have beneficial alleles for hypoxia germination tolerance. qCL9 and qSL5 are major effect QTLs controlling hypoxia germination tolerance and have practical value in the molecular breeding of direct-seeding rice. [ABSTRACT FROM AUTHOR]

The lithology of carboniferous volcanic rocks in Junggar Basin is complicated. It is difficult to divide lithology by conventional methods when the number of thin sections and cores of one lithology is obviously less than that of other lithology. In order to solve the above problems and improve the accuracy of volcanic rock lithology identification, synthetic minority oversampling technique (SMOTE) algorithm was used to increase the number of samples of a few lithologic categories to solve the problem of data imbalance. The optimal parameter combination was determined by grid search and K-fold cross validation method, and the lithologic identification of volcanic rocks based on improved random forest was carried out. By analyzing the data of volcanic rock core, thin section and logging response characteristics, the lithologic crossplot was established, and the importance of logging parameters sensitive to lithology in the study area was determined. The application of case data shows that the improved random forest algorithm effectively solves the influence of the traditional random forest algorithm due to the unbalance of lithological sample types and the small amount of data, and the accuracy rate of volcanic rock lithology identification increases from 87% to 94%, which provides reference for the lithology identification of volcanic rock in the case of unbalanced samples. [ABSTRACT FROM AUTHOR]

In the engineering construction of immersed tube tunnel, the foundation bed bathymetry is an important part of the foundation construction, and high -precision foundation bed bathymetry is an important guarantee for the quality of the foundation engineering. Relying on the gravel leveling test project of the submarine tunnel foundation bed of Shenzhen Zhongshan Link project, the research and application of high-precision detection technology based on multi-beam bathymetry system was carried out. The underwater same position parameter conversion comparison method was used to realize the testing and verification of the accuracy of multi-beam bathymetry system by geodetic high precision instruments. The calibration and error mandatory correction of the data processing model of multi -beam system by setting the underwater elevation ratio measurement steel plate effectively improves the accuracy of foundation bed laying. By comparing the multi-beam sweep data with the results of penetration measurement, the results are basically consistent, which indicates that the multi -beam bathymetry system meets the project needs. The high-precision detection technology of multi-beam bathymetry system for immersed tunnel foundation construction can provide reliable reference data for underwater foundation construction. [ABSTRACT FROM AUTHOR]

Objective 　To observe the antipyretic effect of Tuina antipyretic six methods on lipopolysaccharide (LPS)-induced fever in infant rabbits, and to explore the effects on the hypothalamic COX-2/PGE2/EP3 signaling pathway. Methods 　Using the random number table method, 24 New Zealand rabbits(two months old)were randomly divided into the control group, model group and Tuina six methods group, with eight rabbits per group. The fever model was replicated by LPS (0. 5 mg/L) injection at the ear margin, and the injection volume was 1 mL/kg in model group and Tuina six methods group. In the Tuina six methods group, the following Tuina six methods of antipyretic intervention were used 1 h after LPS injection: opening Tianmen 200 times in 2 min, pushing Kangong 200 times in 2 min, rubbing Taiyang 200 times in 2 min, rubbing Erhougaogu 24 times and pinching three times in 2 min, clearing the Tianheshui 500 times in 5 min, and pushing the spine 300 times in 5 min. Over the same period, the animals in the model and control groups were smeared with the same volume room temperature water in the same are, and grasped without Tuina intervention. The anal temperature was recorded once every 20 min after LPS injection, and the temperature was continuously measured for 180 min. The body temperature of each group was observed, and the maximum body temperature change value and thermal response index (TRI) were calculated. The expression levels of PGE2 and cAMP in the hypothalamus were detected by ELISA, the protein expression levels of COX-2, EP3, and cAMP were detected using Western blotting, and the mRNA expression levels of COX-2, EP3, and cAMP were detected using RT-PCR. Results　Compared with the control group, the anal temperature of the model group was increased (P<0. 01), and the anal temperature of the model infant rabbits in the Tuina six methods group was decreased after the intervention (P<0. 01). The maximum body temperature change value and the TRI in the model group and the Tuina six methods group were higher than those in the control group at 60 min after modeling (P <0. 01). Compared with the model group, there was no difference in the maximum body temperature change value and TRI 60 min after modeling in the Tuina six methods group. Moreover, 180 min after modeling, the maximum body temperature change value and TRI were increased in the model group(P<0. 01), and those were decreased in the Tuina six methods group compared with those in the model group (P < 0. 01). The ELISA result showed that compared with the control group, the expression levels of PGE2 and cAMP in the hypothalamus of the model group were increased (P<0. 01), while those in the Tuina six methods group were lower than those in the model group (P<0. 05, P<0. 01). The result of Western blotting showed that compared with the control group, the expression levels of COX-2, EP3, and cAMP in the hypothalamus of the model group were increased (P<0. 01), while those in the Tuina six methods group were lower than those in the model group (P<0. 05, P<0. 01). The RT-PCR result showed that compared with the control group,the mRNA expression levels of COX-2, EP3, and cAMP in the hypothalamus of the model group were increased (P <0. 01), and those in the Tuina six methods group were decreased, compared with those in the model group (P<0. 01). Conclusion　Tuina antipyretic six methods shows a good antipyretic effect on LPS-induced fever infant rabbits. Its mechanism is achieved by inhibiting the activation of the hypothalamic COX-2/PGE2/EP3 signaling pathway by pyrogen, reducing the level of positive regulatory medium cAMP at the terminal of the body temperature center. [ABSTRACT FROM AUTHOR]

[Objective] GPAT is the first enzyme in the plant glycerolipid synthetic pathway, which may play an important role in oil quality improvement and biomass energy.[Methord] Based on the GPATs obtained from GenBank, ESTs of Gossypium were selected to obtain the cDNA sequence of GbGPAT2 gene by RT-PCR from Haizi 8, which was a homozygous line of Gossypium barbadease. [Result]The full length cDNA of GbGPAT2 was 1294 bp, containing an open reading frame(ORF) of 1113 bp, which encoded a protein of 370 amino acids. Its molecular weight was about 42.4 kDa, and pI 8.72.GbGPAT2 contained a typical conserved LPLAT-LPCAT1-like domain and a NAT-SF superfamily domain.GbGPAT2 putative protein shared 87%-92 % identity with Jatropha curcas and Arabidopsis, respectively.GbGPAT2 shared only 8 % identity with GhGPAT(ADK23938.1, cloned from upland cotton without further information). The bioinformatics analysis of the membranes and subcellular localization showed that GbGPAT2 might be located in the cytoplasm membrane or the chloroplast membrane, with two transmembrane domain(TMD) structures, subcellular localization analysis showed that GbGPAT2-GFP fusion protein was distributed specifically in membranes. The embryo specific expression analysis by using quantitative RT-PCR assay indicated that the expression level of GbGPAT2 reached a peak in the stage 25 days post anther(DPA). [Conclusion] As a result, GbGPAT2 may play an important role in the Gossypium barbadense seed oil synthesis. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of microRNA-30e (miR-30e) on the invasion and migration of gastric cancer and the potential mechanism. Mrthods: The transwell system and the assays of wound healing were used to assess the invasion and migration ability of gastric cell line BGC823. The miR-30e was transfected into BGC823 cell via lipofectamine 2000. Cells were also transfected with empty vectors to serve as controls. The expression of miR-30e was detected by real-time PCR, and western blot was used to assay the expression of Snail, Vimentin, N-cadherin, and E-cadherin which were the markers of epithelial-mesenchymal transition EMT. Results: After the transfection of the miR-30e, which had significantly suppressed the major factors in EMT signaling pathway, such as the expression of mRNA and proteins of Snail, N-cadherin, and vimentin, increased the mRNA and proteins expression of E-cadherin. And the mimic also had suppressed the invasion and migration of BGC823 cell line induced by TGF-β. Conclusion: MiR-30e may be a key target in the EMT process of cancer cells, blocking the EMT process could inhibit the invasion and migration of gastric cancer cells. [ABSTRACT FROM AUTHOR]

With the progress of technology and the depletion of shallow mineral resources, the development and utilization of deep mineral resources will become reality. The deep rocks have complex physical and mechanics properties, and they are often in cycle loading stress condition. The larger pressure of deep groundwater is one of the main safety hazards in deep coal mines. As one of the most common rock types, it is significant to study the mechanical properties and permeability evolution of the roof sandstone for the exploitation of deep mineral resources. In this paper, the roof sandstone of Pingmei 12th Coal Mine with a depth of about 1 050 m has been investigated. The sandstone permeability evolution under stress is researched by cyclic loading acoustic emission (AE) permeability experiment. Based on the stress-strain and damage characteristics, dissipative energy density ratio, the growth rate of accumulated acoustic emission events, the features of fracture surface under different confining pressures, the permeability evolution of the roof sandstone under cycle loading has been summarized. Experimental results show that the effect of stress on rock can be divided into compaction and fracturing. The low stress mainly acts as compaction. The stress, which increases to more than 60% of the yield strength, mainly acts as fracturing. Permeability decreases under compaction of stress and increases under fracturing of stress. The dissipative energy density ratio and damage variable D of the rock sample decreases under compaction and increases under fracturing, which are both positively correlated with permeability evolution. During the whole experiment, the confining pressure acts as a compaction on the rock sample, and as the confining pressure increases, the permeability decreases to a greater extent. The failure mode of the roof sandstone has a significant effect on the permeability at the time of failure. The rupture angle of rock sample decreases with the increase of confining pressure. When the rock sample is destroyed, there are two forms of axial through crack and transverse through crack. When the axial through crack is generated, the permeability will be much larger than the initial permeability. The permeability of the transverse through crack will not change significantly. Based on the permeability evolution of five rock samples, rock permeability has four distinct phase characteristics under stepwise cyclic loading and unloading. The permeability decreases by compaction in the cycle of low stress. With the increase of cyclic stress, the permeability will increase under the fracture and damage. The permeability increases sharply, with the fracture face generated. The stress is greatly reduced after the failure, and the permeability decreases again due to the compaction. [ABSTRACT FROM AUTHOR]

In the experiment. corn starch was used as raw material to prepare two kinds of modified starch as additives for wool oil. The grafted acrylamide carboxymethylated composite modified starch was prepared by grafting two steps of acrylamide and carboxym ethyl at ion，so that the modified starch had the properties of both acrylamide grafted starch arid carboxymethyl starch. The cross-linked starch prepared by using sodium tri me rate as a cross-linking agent could increase the viscosity stability and adhesion of the starch. The prepared modified starch was subjected to infrared spectrum analysis, gel permeation chromatography analysis？ and determination of solubility and other physical and chemical properties？ which provided theoretical and experimental references for the industrial application of starch-based polymer surfactants in wool spinning processing. [ABSTRACT FROM AUTHOR]