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Objective To explore the vitamin D nutritional status and influencing factors of primary and middle school students in poverty areas of Guizhou Province, and to provide scientific basis for the development of targeted nutritional improvement measures. Methods A total of 2,519 students from 4 primary and4 middle schools in 9 key monitoring counties (districts) in Guizhou Province, where the Nutrition Improvement Program for Rural Compulsory Education Students was implemented in 2023, were selected for questionnaire surveys, physical examinations, and blood biochemistry tests using multistage cluster sampling methods. Multifactorial logistic regression was used to analyze the current status of vitamin D nutrition and its influencing factors. Results The average vitamin D level of primary and middle school students in poverty areas of Guizhou Province was (22. 59 ± 6. 66) ng/ml, and the rate of low vitamin D was 37. 51%. The prevalence of low vitamin D among female students (46. 17%) was higher than that of male students (29. 11%), and the difference was statistically significant 仗2 = 78. 213, P < 0. 001). There was a significant difference in the rate of low vitamin D among students in different regions 仗2 二 23. 454, P <0. 001), and the rate of low vitamin D tended to increase with age = 44. 905, P <0. 001). The results of multifactorial logistic regression analysis showed that, compared with the corresponding reference group, ethnic minorities (OR = 0. 706, 95% CI: 0. 590 - 0. 844), physical activity M60 min/d (OR = 0. 791, 95% CI: 0. 663 - 0. 943), and living in the countryside (OR - 0. 747, 95% CI: 0. 616 -0.906) were protective factors for low vitamin D levels; obesity (OR =2. 133, 95% C/: 1.390 - 3.272), female students (OR 二2. 238, 95% CI: 1.882 -2.662), the 11-13 years old group (OR =1. 849, 95% CI: 1. 521 - 2. 248) and the 14 - 17 years old group (OR = 2. 000, 95% CI: 1. 576 - 2. 539) were risk factors for low vitamin D levels. Conclusion High rates of low vitamin D among primary and middle school students in poverty areas of Guizhou Province require the development of personalized nutritional interventions to improve vitamin D nutritional status. [ABSTRACT FROM AUTHOR]

Objective: To prepare anti-human IgE nanobody by phage display technology, and to establish a method for hypersensitivity detection of cat dander specific IgE antibody. Methods: Allergen bio-information of cat was searched in WHO/IUIS Allergen Database. After synthesizing sequence, recombinant cat dander allergenic protein Fel d 1 was expressed and purified in prokaryotic expression system. Human IgE was used to immunize Bactrian camel and RNA were extracted from lymphocyte to construct phage display library. Library capacity, diversity and insertion rate were analyzed, anti-human IgE nanobody were obtained by screening and identification. A magnetic particle chemical method for cat dander specific IgE antibody detection was established using recombinant allergen-coupled magnetic particles and acridine ester-labeled nanobodies. Results: Capacity of phage display library was 1.88×108 cfu/ml, insertion rate was 93.6%, and purity of nanobody was >95%. Linear range of the method based on nanobody was 0.1~100 U/ml, who was consistent with ImmunoCAP detection system by clinical data. Conclusion: Nanobody-based cat dander specific IgE antibody hypersensitivity assay is successfully prepared, providing a technical basis for auxiliary diagnosis of cat allergic diseases. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between 25-hydroxyvitamin D3 [25 (OH) D3] levels and insulin resistance, urine pH, and other factors in newly diagnosed type 2 diabetes mellitus (T2 DM) patients. Methods The levels of 25 (OH) D3 were measured in 325 non-diabetic patients (normal control group, n=325) and 208 newly diagnosed T2 DM patients (newly diagnosed T2 DM group, n=208) .Based on the levels of 25 (OH) D3,newly diagnosed T2 DM patients were divided into vitamin D deficiency group (n=92) and non-deficiency group (n=116). The level of25 (OH) D3 was considered deficient if it was ＜20 ng/mL and sufficient if it was 20-100 ng/mL. The correlation between 25 (OH) D3 and insulin resistance, urine pH, etc., was analyzed using SPSS 26.0.Results The level of 25 (OH) D3 in the newly diagnosed T2 DM group was higher than that in the normal control group (P ＜0.01); the age, glycated hemoglobin, insulin resistance index, urine pH, free triiodothyronine (FT3), and free thyroxine (FT4) in the deficiency group were higher than those in the non-deficiency group (P ＜0.05), while there was no statistically significant difference in pancreatic function index, fasting C-peptide, serum calcium, and thyroid-stimulating hormone (TSH) between the two groups (P＞ 0.05). Logistic regression analysis showed that glycated hemoglobin (OR=1.776, P ＜0.05) and insulin resistance index (OR=1.289, P ＜0.05) were risk factors for vitamin D deficiency in newly diagnosed T2 DM patients. Conclusion The level of 25 (OH) D3 in the newly diagnosed T2 DM group was lower than that in the normal control group. Higher glycated hemoglobin and insulin resistance index are risk factors for vitamin D deficiency in patients with T2 DM. [ABSTRACT FROM AUTHOR]

Saline-alkali soil improvement is one of the important measures to enhance the production capacity in sustainable agriculture. Fulvic acid can serve as an ecologically friendly soil conditioner in the saline-alkali soil treatment, in order to improve soil fertility, crop yield, and quality. Biochemical humic acid extracted from the straw has demonstrated a superior improvement on the saline-alkali soil, compared with the mineral-derived humic acid, thus offering an effective way for the straw resource utilization. This study aims to explore the improvement mechanism of biochemical fulvic acid on the soda salinealkali soil with different textures. A systematic investigation was also implemented to assess the impact on water-salt transport characteristics. The indoor one-dimensional vertical infiltration experiments were then conducted to explore the water-salt transport, infiltration model parameters, and eight major salt separators. Different amounts of biochemical fulvic acid [0 (CK), 1, 2, 4, and 8 g/kg] were applied in the soda saline-alkali soil with various textures. The results showed that the cumulative infiltration amount decreased by 3.70%, 10.19%, 12.04%, and 25.00% in the sandy loam soil for the treatments with the biochemical fulvic acid of 1, 2, 4, and 8 g/kg, respectively, after 280 min of infiltration compared with the CK treatment. The wetting front depth also decreased by 9.33%, 17.00%, 24.00%, and 27.33%, respectively. In the loamy sandy soil, the cumulative infiltration amount of the treatments with the biochemical fulvic acid of 1, 2, 4, and 8 g/kg decreased by 1.36%, 10.51%, 18.98%, and 29.83%, respectively, after 40 min of infiltration compared with the CK treatment, with the wetting front depth reductions of 5.00%, 11.33%, 21.67%, and 31.33%, respectively. The infiltration rate of soil moisture decreased with the increase in the application amounts of biochemical fulvic acid. At the soil depths of 0, 5, 10, 15, 20, 25, and 30 cm, the sandy loam treated with 8 g/kg showed a higher soil moisture content than the CK treatment by 11.43%, 4.99%, 8.54%, 8.79%, 9.02%, 4.98%, and -7.76%, respectively. Similarly, the loamy sandy exhibited a higher soil moisture content than the CK treatment by 4.76%, 9.28%, 6.18%, 9.25%, 8.05%, 8.77%, and -0.06% at the corresponding depths, respectively. Both the Kostiakov and Philip models were used to effectively simulate the infiltration, indicating the gradual decrease in the infiltration rate with the increasing application amounts of biochemical fulvic acid. Soil infiltration also decreased the soil pH value. Specifically, the soil pH value increased with the increase of application amounts, with the most significant change in soil pH, compared with the CK treatment at 8 g/kg application amount. Moreover, the application of biochemical fulvic acid increased the soil salt content at various depths, potentially leading to elevated Na+ concentrations in soils with low Ca2+ and Mg2+ concentrations. Therefore, the application of biochemical fulvic acid significantly influenced the water and salt transport characteristics and then improved the water distribution for the reduced pH value in the soda saline-alkali soil with different textures. To avoid further secondary salinization caused by the introduction of salt ions via biochemical fulvic acid, the application rate of 2-4 g/kg was recommended for better improvement of soda saline-alkali soil with varying textures. Therefore, the finding can serve as the theoretical foundation and support for the green management of saline-alkali soil using biochemical fulvic acid. [ABSTRACT FROM AUTHOR]

The coupling coordination of scientific and technological innovation, ecological environment, and economic development is an effective way for China to achieve high-quality development in the new development stage. The new infrastructure construction (hereinafter referred to as 'new infrastructure') with digital technology as the core provides new momentum for China's high-quality coordinated development. Guided by the new development concept and based on system theory and synergy theory, this study constructed a coupled coordination system of 'innovation-ecology-economy,' employed inter-provincial panel data from 2011 to 2020, and combined the spatial Durbin model with the mediating effect test method to empirically test the impact of new infrastructure on regional 'innovation- ecology-economy' coupling coordination. The findings indicated that: ① New infrastructure investments improved the level of regional 'innovation-ecology-economy' coupling coordination, generating strong positive spatial spillover and supporting both geographic distance matrix and economic distance matrix. ② Different types of new infrastructure investments contributed to the regional 'innovation- ecology-economy' coupling coordination, showing significant heterogeneity, where innovation infrastructure and convergence infrastructure had significant driving effects, while information infrastructure had a less obvious enabling effect. ③ New infrastructure investments fostered regional 'innovation-ecology-economy' coupling coordination through three transmission mechanisms: innovation factor concentration, energy efficiency improvement, and digital financial boost. Accordingly, Chinese provinces should enhance the enabling effect of new infrastructure on the regional 'innovation-ecology-economy' coupling coordination by increasing new infrastructure investment, promote the formation of a linkage mechanism for new infrastructure empowerment, and open up the transmission path of new infrastructure so as to achieve high-quality and sustainable regional development in the new era of China. [ABSTRACT FROM AUTHOR]

In order to investigate the expression differences of Lonicera caerulea fruit genes at different developmental stages（green ripening stage and ripening stage）and to explore the key metabolic pathways of L. caerulea during growth and development, the transcriptome data of L. caerulea at green ripening stage and ripening stage was compared by using high-throughput sequencing technology with‘ Zhongkelan No.1’ variety as the experimental object. The results showed that there were 40.32 Gb Clean Data, each sample had > 6.07 Gb Clean Data, the percentage of Q30 bases was 93.89% and above, and the GC content ranged from 46.32% to 49.38%. There were 3 247 differentially expressed genes detected in the fruits of the two developmental stages, including 1 642 up-regulated genes and 1 605 down-regulated genes. GO functional enrichment analysis revealed that the key regulatory genes during the development of L. caerulea were mainly involved in metabolism, cellular and cellular components and catalytic activity. KEGG enrichment analysis revealed that the differentially expressed genes were mainly enriched in plant hormone signaling, carbon metabolism and amino acid biosynthesis. These results provide a reference for further studies on the dynamic changes during fruit development of L. caerulea, and provide a theoretical basis for subsequent studies on the identification of functional genes and genetic diversity analysis of L. caerulea. [ABSTRACT FROM AUTHOR]

Objective To investigate the ABO blood type distribution in liver cancer patients in Qidong District of Jiangsu Province, and to explore the relationship between ABO blood type and the risk of liver cancer. Methods Peripheral venous blood was collected from 1 701 patients with liver cancer and 2 828 healthy persons from Qidong after disease exclusion to detect ABO blood type, and the relationship between ABO blood type and risk of liver cancer was analyzed. Results The frequency distributions of blood types A, B, O, AB were 518 (30. 45%)，436 (25. 63%)，518 (30. 45%), and 229 (13. 46%), respectively, among liver cancer patients, and 862 (30. 48%)，818 (28. 93%)，853 (30. 16%), and 295 (10. 43%), respectively, among healthy persons. Compared with healthy persons, the distribution of blood type AB was higher and B distribution was lower in liver cancer patients (both P<0. 05). Qidong population with blood type B had a low risk of liver cancer (OR=0. 847，95%CI：0. 739–0. 970）, and the population with blood type AB had a high risk of liver cancer (OR=1. 336，95%CI：1. 111–1. 606). Compared with healthy persons, the distribution of blood type B was lower and the distribution of AB type was higher in male liver cancer patients （χ2 =5. 618，11. 01； OR=0. 809，1. 534, respectively； both P<0. 05). Conclusion ABO blood type is related to the occurrence of liver cancer in Qidong. The risk of liver cancer is higher in the population with blood type AB and is lower in the population with blood type B. [ABSTRACT FROM AUTHOR]

Based on the pressure-state-response (PSR) model, the evaluation system of urban ecological security was established, and an improved cloud matter-element model was proposed. By using the double uncertainty of cloud model and matter-element extension theory, the comprehensive evaluation of urban ecological security in Shandong Province from 2009 to 2019 was carried out, and the ecological security and index contribution of Jinan City were mainly studied. The results show that the ecological security index given by the model can not only describe the ecological security situation, but also compare different cities. Although Jinan has been in a critical state of security, the ecological security index has been rising steadily, which is greatly affected by the pressure and state of the ecosystem. The ecological security status of 16 prefecture level cities in Shandong Province has obvious differences, and the overall trend is on the rise, but the overall level is in the critical security level, Weihai, Qingdao and Jinan are in the forefront, while Dezhou, Zaozhuang and Heze are less secure. Shandong provincial government should pay attention to the imbalance of regional development and formulate relevant policies to narrow the gap between cities. [ABSTRACT FROM AUTHOR]

Medical oncology is a subject characterized by drug therapy. Continuous research and development (R&D) of high-efficiency and low-toxic anti-cancer drugs is the premise of the development of medical oncology. Clinical trials play an indispensable role in the process from drug R&D to application, which determines the success or failure of a new drug. The clinical trials for anti-cancer investigational new drug (IND) in China began in 1960, and have developed rapidly since 2008. With the guidance and support of national policies, as well as involved by all aspects of the society, the R&D of anti-cancer drugs in China has changed from imitation to innovation. China innovative anti-cancer drugs have been widely recognized in the world, and more and more new domestic anti-cancer drugs have been used in clinical practice, bringing benefit to Chinese cancer patients. This article reviews the development of the clinical trials for anti-cancer IND in China from 1960 to 2020, and the main achievements having been made in the past 60 years. A thorough understanding of this history will help us keep in mind the mission and grasp the direction, as to make more achievements when implementing the strategy of "Healthy China" . [ABSTRACT FROM AUTHOR]

Currently, food security, rural revitalization, and ecological civilization construction are important issues in agricultural and rural areas. The carrying capacity of cultivated land resources is a fundamental problem. This study summarized the concept, origin, and development of the carrying capacity, and systematically summarized four representative evaluation models（AEZ method, ecological footprint method, model index method, and comprehensive evaluation method）. Four methods of influencing factors index system and key influencing factors identification were summarized, and their advantages and disadvantages were compared. The results showed the index system of influencing factors mostly appeared in the large- and medium-scale research on cultivated land resource carrying capacity, and it was easy to build; however, in small-scale research, such as village units, no representative research results had been formed. The identification of key influencing factors was mainly based on subjective methods, such as expert consultation method and lack of theorem method selection. The trend of the cultivated land resources carrying capacity evaluation model was the selection of comprehensive research methods. Therefore, using a comprehensive evaluation model should be prioritized in future research method selection. [ABSTRACT FROM AUTHOR]

目的总结急性巨核细胞白庄病( Acute megakaryoblastic leukemia, AMKL) 的临床特点，探讨其有效治疗 方法。方法收集4 例婴幼儿AMKL 患儿的临床资料作回顾性分析。结果4 例患儿中男1 例、女3 例， 2 例为新 生儿起病， 2 例为幼儿期起病。主要临床表现为贫庄、感染。4 例患儿血清骨髓细胞小巨核酶标均为阳性。3 例患 儿白血病免疫分型中CD41 、CD61 表达阳性，2 例锁系抗原CD13 、CD33 阳性， 1 例T 淋巴细胞抗原CD9 、CD36 阳性。 3 例患儿染色体核型分型为21 三体综合征2 例、复杂多倍体核型1 例。2 例患儿接受阿糖胞昔、蔥环类药物化疗方 案治疗， 随访中患者均无事件生存。结论AMKL 的主要临床表现为贫血、感染， 患儿存在巨核细胞特异性标志 CD41 、CD61 表达， 还可存在髓系抗原(CD13 、CD33 ) 及T 淋笆细胞抗原( CD9、CD36) 表达。21 三体综合征可能是 AMKL 的易感人群， 非21 三体综合征的AMKL 患儿染色体核型多为复杂核型。阿糖胞有、蔥环类药物化疗方案治 疗AMKL有效。 [ABSTRACT FROM AUTHOR]

As an important branch of traditional Chinese medicine，Lingnan academic school has unique styles in the treatment of diseases caused by exogenous pathogenic factors. By using textual research methods, this paper analyzes geographical characteristics and body constitution in Lingnan area. The external factors (high temperature and high humidity，irregular seasonal climates and residual Yangqi) shape the unique constitutional characteristics of " yang qi floating exteriorly with yin qi closing interiorly". Taking these factors into consideration? local practitioners have developed the unique treatment approaches and preferences ? such as frequent and flexible use of dampness-drying and centerwarming medicines at low doses ? rare use of diaphoretic and purgation medicines and emphasis on " homology of medicine and food ". The representative clinicians were Liu Shichang and Zhen Mengchu etc. [ABSTRACT FROM AUTHOR]

目的探讨血浆纤维蛋白原与白蛋白比值(FAR)对非ST段抬高型急性冠脉综合征(NSTE-ACS)患者PCI术后发生不良心血管事件(MACE)的预测效能。方法回顾性分析2018年1月～2019年6月徐州医科大学附属医院经皮冠状动脉介入(PCI)治疗的NSTE-ACS患者1 048例,根据患者出院后6个月是否发生MACE分为观察组(101例)和对照组(947例),采用单因素和多因素Logistic回归分析法分析行PCI治疗的NSTE-ACS患者术后发生MACE的影响因素、危险因素,绘制ROC评估血浆FAR、全球急性冠状动脉事件注册风险评分(GRACE评分)及两指标联合预测NSTE-ACS患者PCI治疗术后发生MACE的价值。结果单因素分析结果显示,FAR、DM比例、年龄、GRACE评分、ALB、FIB是行PCI治疗的NSTE-ACS患者术后发生MACE的影响因素(P均<0.05);多因素分析结果显示,FAR、GRACE评分、年龄是行PCI治疗的NSTE-ACS患者术后发生MACE的危险因素(P均<0.05)。ROC分析显示,FAR在最佳截点>8.09时预测MACE的灵敏度为77.2%、特异度为56.7%,GRACE评分在最佳截点>137时预测MACE的灵敏度为79.2%、特异度为77.4%,两者联合在最佳截点>0.09时预测MACE的灵敏度为85.1%、特异度为78.2%。结论年龄、FAR、GRACE评分是MACE的独立危险因素,术前FAR水平对NSTE-ACS患者PCI术后发生MACE事件有一定的预测价值。 [ABSTRACT FROM AUTHOR]

In this study, a cadmium (Cd)-resistant bacterium D36 was isolated from the rhizo- spheric soil of tomato plants. The 16S rDNA gene was amplified and the plant growth-pro-moting properties were identified ? the effects of D36 on the growth and Cd accumulation in tomato plants were investigated via hydroponic experiment. The results indicated that the ob¬tained strain belongs to the genus of Sphingomonas and it has phosphate solubilization ? sid- erophores production and indoleacetic acid production properties. Under Cd stress?D36 inocu¬lation increased the root length and plant height of tomato plants by 210. 74% and 14. 97%. The fresh weight of the shoots and roots was increased by 18. 33 % and 15.09%? respectively. Total chlorophyll content was increased by 13. 40%. Meanwhile? the H2O2 content was decreased by 35. 09 % in tomato roots and the POD activity was increased by 74. 94%. Be sides,D36 inoculation markedly decreased the Cd content in tomato roots by 27. 63%. Taken together,our results indicated that D36 could modulate growth and Cd uptake of the tested plants. This study might provide a promising way of using PGPR control Cd uptake in food crops. [ABSTRACT FROM AUTHOR]

BACKGROUND: Chronic graft-versus-host disease is the most common complication after transplantation and glucocorticoid is a first-line drug. Glucocorticoid in combination with immunosuppressive therapy is not effective in half of the patients with hormone resistant chronic graft-versus-host disease. The low immunogenicity of umbilical cord mesenchymal stem cells provides the possibility for clinical treatment of graft-versus-host disease. OBJECTIVE: To investigate the clinical efficacy and safety of umbilical cord-derived mesenchymal stem cells to treat refractory chronic graft-versus-host disease. METHODS: Fifteen patients with refractory chronic graft-versus-host disease received mesenchymal stem cell infusion treatment based on immunosuppressive therapy. The therapeutic efficacy, infusion-related adverse reactions, and survival were analyzed. The ratio change of peripheral blood lymphocytes was determined by flow cytometry. This study was approved by Medical Ethics Committee, Third Affiliated Hospital of Sun Yat-sen University in China. RESULTS AND CONCLUSION: There were 12 male and 3 female patients with a median age of 29 years (ranging from 17 to 52 years). Four patients obtained complete response, seven patients obtained partial response, 11 had overall response, and four patients had no response. After treatment by umbilical cord mesenchymal stem cells, the ratio of CD19+ cells in the peripheral blood was slightly, but not significantly lower, but CD19+CD27+ and CD3+ cell ratios were slightly, but not significantly higher than those before treatment. No patients had adverse reactions related to infusion of umbilical cord mesenchymal stem cells and no patients had primary disease recurrence and mesenchymal stem cell-related tumor. These findings suggest that umbilical cord-derived mesenchymal stem cell infusion is an effective and safe therapy for refractory chronic graft-versus-host disease. [ABSTRACT FROM AUTHOR]

Representation learning is to map the information with association relationship into a lowdimensional vector space through a shallow neural network in machine learning. The goal of word representation learning is to map the relationship between words and their context words into the low-dimensional vector, while the goal of network representation learning is to map the relationship between network nodes and context nodes into the low-dimensional vector. The word vector is the output of the word representation learning, and the node representation vector is the output of the network representation learning. DeepWalk obtains the walk sequence in the network as a sentence of word2vec model through a random walk strategy, and the node pair is trained in the neural network through the sliding window. word2vec and DeepWalk use the same underlying model and optimization method: Skip-Gram model and negative sampling optimization method. In word2vec and DeepWalk, the Skip-Gram model with negative sampling is called SGNS. The existing research results show that both the word representation learning and the network representation learning algorithm based on the SGNS model both implicitly decompose the target feature matrix. Perozzi et al. proposed that word frequency obeys Zipf's law and node degree in the network obeys the power law distribution, and they considered that the random walk sequence of the network is equivalent to the sentence of the language model. However, the reason for judging whether a sentence is equivalent to a random walk sequence is only based on the power law distribution, which is not sufficient. Therefore, based on the theory and basis of SGNS's implicit decomposition of the target feature matrix, this paper designs two comparative experiments. The experiments use singular value decomposition and matrix completion method to perform node classification tasks on three public data sets, and confirms the equivalence between sentences and random walk sequences. [ABSTRACT FROM AUTHOR]

For switched reluctance motor, it shows high torque ripple and large vibration amplitude, an improved structure of switched reluctance motor was proposed, which is to add pole shoes on both sides of stator teeth and slots on both sides of rotor poles. By adding pole shoes on both sides of stator teeth, the minimum inductance of the motor was increased to increase the minimum torque during commutation, so as to reduced the torque ripple of the motor. Slots were made on both sides of the rotor to change the direction of the air gap magnetic density, increased the tangential component of the air gap magnetic density, and reduced the radial component of the air gap magnetic density, by reducing the wave height of the radial force, the noise of the motor was reduced. For the determination of the optimal values of pole shoe parameters and slotting parameters, were cooperatively optimized the control parameters (turn-on angle and turn-off angle) and structural parameters (pole shoe parameters and slot parameters) were cooperatively optimized based on the improved non-dominated sorting genetic algorithms-II(NSGA-II). Through simulation and experiment, it is proved that compared with the initial model, the average torque of the optimized motor is improved, the torque ripple is reduced, and vibration of the motor is greatly reduced. [ABSTRACT FROM AUTHOR]

With the support of the national policies, the improvement of research ability and the development of economy and society, China clinical trials have developed rapidly. Many achievements are made in the clinical trials of new anti-cancer drugs during last several decades. Many innovative new drugs have come into the market and gained influence at home and abroad. Those drugs provide more treatment options for Chinese patients. This article reviews the results of new anti-tumor drug clinical trials, with special focus on the challenge and chance for the new anti-cancer drug clinical trials in China. [ABSTRACT FROM AUTHOR]

Objective To explore the secondary transplantation strategy for graft failure of the primary haploidentical hematopoietic stem cell transplantation (haplo-SCT) associated with fatal complications in a patient with acute T-cell lymphocytic leukemia. Methods One male patient was diagnosed with acute T-cell lymphocytic leukemia. In November 2015, he received haplo-SCT from his sister as the donor. But graft failure was developed and complicated by Escherichia coli sepsis, serum disease, hepatic venous occlusion and multiple organ failure. At 32nd d after the first transplantation, secondary haplo-SCT from his father as the donor was performed. Prior to secondary transplantation, chemotherapy pretreatment was not conducted. Post-transplantation cyclophosphamide (PTCy)-based prevention measure against the graft-versus-host disease (GVHD) was adopted after transplantation. Results White blood cells were implanted on the 12nd d and platelet implantation was performed on the 67th d after haplo-SCT. Serum disease, septicemia and hepatic venous occlusion were cured and organ function was improved. However, the patient died from the recurrence of leukemia at 18th months after transplantation (June 2017). Conclusions Prior to secondary haplo-SCT, PTCy prevention measures against the GVHD rather than pretreated chemotherapy probably contributes to the success of transplantation. [ABSTRACT FROM AUTHOR]

The lectures of network programming and middleware are very importantly. These lectures are based on Java language. This paper interpreted conception of communication and middleware. Using instance of Web Java project, it showed ideas and steps of Web Java application depend on concept of network communication architecture and data encapsulation process. Students could master the Java network programming. [ABSTRACT FROM AUTHOR]

Objective To observe the efficacy of autologous hematopoietic stem cell transplantation on the treatment of patients with acute myeloid leukemia (AML) in complete remission stage. Methods Clinical data of 14 AML patients underwent autologous hematopoietic stem cell transplantation were analyzed retrospectively, including 7 low-risk patients, 6 moderate-risk patients and 1 high-risk patient. After pretreatment, pre-cryopreserved autologous peripheral blood stem cells were retransfused. And component blood transfusion, increasing white blood cell (WBC) count and preventing from infection, etc. were given. Hematopoietic reconstitution of autologous stem cells in the patients was observed, and incidence of transplantation related complications was obtained. Furthermore, survival curves were drawn, and postoperative 1- and 3-year overall survival rates and disease-free survival (DFS) rates were calculated. Results Hematopoietic reconstitution was achieved in all 14 patients. The median time of WBC implantation was 12(9-28) d, and that of platelet implantation was 29(8-158) d. Two patients suffered from E. coli septicemia during neutropenia stage, 1 from proteus vulgaris septicemia, 1 from cytomegalovirus viremia within 29 d after transplantation and the remaining from infection or gastrointestinal reaction after pretreated. All patients were cured by anti-infection and other symptomatic relief and supportive treatment. All patients were followed up for 29.8(5.3-61.5) months, among which 11 patients survived and 3 died of recurrence. The 1- and 3-year overall survival rates were 86% and 79%, and the 1- and 3-year DFS rates were 64% and 57%. Conclusions Autologous hematopoietic stem cell transplantation is effective in the treatment of majority patients with low- or moderate-risk AML. [ABSTRACT FROM AUTHOR]

[Objective] This paper aimed to explore occurrence status and species of root knot nematodes in kiwifnjit orchards and lay a foundation for control of Meloidogyne incognita in Xiuwen county, Guizhou. [Method]The morphology, rDNA-ITS and specific sequence of root knot nematodes collected from main producing region of kiwifnjit in Xiuwen county were analyzed and identified. [Result] The perineal pattern of female root knot nematode adult had a high and square-shaped dorsal arch composed of wavy smooth lines from thin to thick and some lines were bifurcated on the side without an obvious lateral line. The similarity between sequence of specific amplified fragment and sequence of Meloidogyne incognita in NCBI Database (Accession number ;JN005841. 1. 1) reached 100 %,which indicated that the root knot nematode belonged to Meloidogyne incognita. [Conclusion] The root knot nematode endangering kiwifruit is Meloidogyne incognita mainly in Xiuwen county, Guizhou. [ABSTRACT FROM AUTHOR]

With the wide and rapid advent of portable/wearable medical devices, the collected long-term health data corpus provides a great opportunity to improve the quality of cardiac care. Based on the previous work on ECG clock and an open source tool, we researched how to create easy-to-interpret plots for cardiac information over along period.ECG clock library was drawn from a dataset of 24-hour Holter recordings. In relevant cardiac diagnose,normally physicians and researchers focus on the heart rate and QT dynamics which were tested in hospital, and the result show reliability. It can provide new insights into circadian patterns of cardiac function in individuals and groups. [ABSTRACT FROM AUTHOR]

长链非编码RNA(lncRNA)具有复杂的生物学功能,参与了非小细胞肺癌(NSCLC)中多个信号转导通路,异常表达在NSCLC发生、发展过程中具有重要的作用。单独lncRNA作为NSCLC诊断标志物的敏感性及特异性较低,多个lncRNA联合诊断敏感性及特异性较高,但目前尚无统一公认的一组lncRNA。针对lncRNA治疗NSCLC的手段包括通过RNA干扰技术介导的基因沉默治疗、反义寡核苷酸介导的治疗、以质粒为基础的靶向治疗、小分子抑制剂介导的对lncRNA的调控等。多个lncRNA与NSCLC患者预后密切相关,但尚无可作为NSCLC预后判断的lncRNA。 [ABSTRACT FROM AUTHOR]

Cervical insufficiency is one of the leading causes of middle-late stage abortion and premature birth. Cervical cerclage may be the main operation method to treat and the effect has been the hot topic for the scholars both at home and abroad. Emergency cervical cerclage is in order to prolong the immature fetal gestational age under the emergency circumstances. But it remains controversial in single or multiple pregnancy, choosing of operation type, the delivery method , the treating of complications , the management of peri-operative and pregnancy outcome. We want to discuss the application value of the emergency cerclage and provide the treatment basis for the clinical in this paper. [ABSTRACT FROM AUTHOR]

Inflorescence stem mechanical strength and some elements contents of herbaceous peony (Paeon,ia lactiflora Pall. ) within three categories were measured. The results showed that the contents of Ca, K, Mg, Cu, Zn and Si were characterized by high mechanical strength variety > medium mechanical strength variety > low mechanical strength variety, the content of Fe and Mn in contrast. Among them, Ca K, Mg and Si content were significant differences between the type, and the others were not significant difference between the types. Correlation analysis showed that stem mechanical strength of the high mechanical strength class significantly positively correlated to Ca, K and Sicontents, and the medium together with low one significantly positively correlated to Ca and Si contents. Mechanical strength positively correlated to Cu contents, and significantly associated with medium mechanical strength varieties. The correlation was different in stem mechanical strength and Mg, Fe, Mn, Zn contents. [ABSTRACT FROM AUTHOR]

目的 探讨选择性痔上黏膜切除术（ＴＳＴ）联合聚桂醇注射液对Ⅱ、Ⅲ期内痔及混合痔患者的治疗效果及不良反应。方法 选择Ⅱ、Ⅲ 期内痔及混合痔患者７０ 例，随机分为对照组、治疗组，各３５ 例，对照组以ＴＳＴ 治疗， 治疗组以ＴＳＴ 联合聚桂醇注射液治疗，根据病情注射２ ～４ ｍＬ／次。观察两组围术期情况、临床疗效、不良反应及复发情况，并检测手术前后肛管直肠动力学指标。结果 与对照组比较，治疗组术中出血量少，术后住院时间与伤口愈合时间短（Ｐ 均＜０． ０５）。对照组治愈率与总有效率低于治疗组（Ｐ 均＜ ０． ０５）。治疗组术后 ＶＡＳ 评分及镇痛剂 使用频率均低于对照组（Ｐ 均＜０． ０５）。两组手术前后及两组间肛管直肠动力学指标比较，Ｐ 均＞ ０． ０５。对照组不良反应发生率及复发率高于治疗组（Ｐ 均＜０． ０５）。结论 ＴＳＴ 联合聚桂醇注射液治疗Ⅱ、Ⅲ 期内痔及混合痔的临床效果好，治疗时间短，并发症少，复发率低；且可减缓术后疼痛，不良反应少 [ABSTRACT FROM AUTHOR]

Educational activities have obvious influence on the development of social space, which has always been an important topic of theoretical and practical research. In China, with urban economic development and social transformation, the differentiation of residential space caused by differentiation of educational resources is becoming more pronounced. School-district communities in many central cities, especially the high-quality school-district communities in old urban areas, with a strong contrast between high housing price and dilapidated material landscape, high-quality education and poor living environment, have a strong pulling and tearing effect on the value orientation and social practice of individuals, families, and communities. This has become one of the most typical spaces of social-spatial contradiction and distortion. This study introduces the concept of "alienation" (a philosophical term) and investigates the social-spatial characteristics and mechanism of highquality school-district communities, from the theoretical viewpoint of "social-spatial dialectical unity." Taking Nanjing as an example, a survey reveals that a large proportion of the high-quality school-district community is old and dilapidated. Hence, the contrast between their material space and social value is significant, the housing price is at an irrational premium, the educational function surpasses the residential function and becomes the primary function of the community, and residents construct a non-geographical social network and shape an education community neighborhood. The high-quality school-district community in Nanjing exhibits the characteristics of social-spatial alienation, which is common and significant. This study further analyzes the mechanism of alienation, which is logically related to external factors such as housing market reform, school district, and oriental culture. However, in essence, the social-spatial alienation is the result of the impact on the dialectical unity of society and space, displaying the tendency of separation and opposition, which promotes the self-denial and role evolution of the community of "high-quality school-district housing". The social-spatial alienation will lead to many negative effects. It will lay siege to the school district and aggravate the antagonistic effect of society and space. It will trigger the solidification and intergenerational transmission mechanism of social stratification and residential differentiation, leading to the residential re-differentiation. It will encounter such difficulties as the absence of subjects, the misplacement of objectives and the rupture of the mechanism, resulting in the "failure" of school-district community governance. It will highlight the contradictions such as high renewal cost, diversified and complex stakeholders, and resistance to school-district rezoning, and delay the process of renewal of the old city. Based on the philosophical construction of "alienation", the social space characteristics of high-quality school-district community are summarized, which provides a new perspective for the study of urban social space. This study also systematically analyzes and explains the mechanism of socialspatial alienation in high-quality school-district communities, and provides tools for the problems in the transformation and reconstruction of urban social space in China. [ABSTRACT FROM AUTHOR]

According to the feature of Tibetan murals, a compressed sensing algorithm is presented based on wavelet. The improved compressed sampling algorithm separates the high frequency sub-bands from the low frequency sub-band, and realizes sparse transformation. The compressed sensing OMP algorithm is employed in image reconstruction. Experiments show if the number of blocks and random matrix is fixed, then the sampling rate is higher, the PSNR will be smaller, and the reconstructed image will have better quality. Hence the algorithm is effective for Tibetan murals. [ABSTRACT FROM AUTHOR]

Server and Dynamic Web Technology is a course for network engineering. In the course， students learn to implement dynamic web sites and link it to the database software based on Microsoft Visual Studio 2005，and develop a dynamic collection of web pages on XP environment. When dynamic pages are posted on the network operating system Windows Server 2003 Web server，students need to install a network operating system on one machine，and then set up the Web server to install the database environment. Another computer is needed as a workstation for developing dynamic pages. In most laboratories，it is difficult to provide two machines to each student. VMware is used for installation of Windows Server 2003，Web server，Mysql database and VS. Dynamic pages based on VS thus can be published on the Web server. Experiments prove that this scheme is simple yet efficient. It enhances students practical and comprehensive ability related to the course. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of unrelated cord blood transplantation (UCBT) on the treatment of high-risk childhood and adult acute leukemia. Methods Ten patients with high-risk acute leukemia underwent UCBT. Among the 10 patients, 3 were children and 7 were adults with the median age of 29 years old (11-41 years old). Six patients underwent one-unit cord blood transplantation and four patients underwent two-unit cord blood transplantation. The myeloablative conditioning regimen without antithymocyte globulin (ATG) was adopted. Cytarabine (Ara-C), fludarabine (Flu) or total body irradiation (TBI)was added on the basis of busulfan (Bu) and cyclophosphamide (Cy). Ciclosporin and mycophenolate mofetil were used to prevent graft-versus-host disease (GVHD). Results The transplantation was successful in 8 (80%) patients. The median implant-time of leukocytes was 19 d (1 4-25 d) and that of platelets was 40 d (33-60 d). Three patients developed acute GVHD and no patient developed chronic GVHD. The median follow-up time was 24 months (1 -29 months). Seven patients remained in disease-free survival. Both the 2-year overall survival and disease-free survival rates were 66.7%. Conclusions UCBT is feasible in the treatment of high-risk acute leukemia. UCBT is the preferred option for the high-risk patients without HLA-identical sibling donors, which is characterized by low incidence of GVHD and low recurrence rate. It may make patients with acute leukemia remain long-term survival. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the status of outpatient prescriptions and irrational prescription in Zhengzhou Orthopaedic Hospital (hereinafter referred to as “our hospital”), so as to provide reference for the rational drug application in clinic. METHODS: By using random sampling method, 1 day of outpatient prescriptions in each month were randomly extracted from our hospital in 2016, totally 5 166 prescriptions. The prescriptions were commented and analyzed according to the relevant national regulations, drug instructions, expert consensuses and drug use guidelines. RESULTS: Of the 5 166 outpatient prescriptions, the application rate of national essential drugs was 44.76%, the application rate of common name was 100%, the application rate of antibiotics was 7.43%, the application rate of injections was 12.74%, the average drug categories was 2.62 and the average consumption sum was 313.53 yuan. There were 380 cases of irrational prescriptions, mainly performed as non-standard prescriptions (265, 69.74%), improper drug-use prescriptions (75, 19.74%) and abnormal prescriptions (40, 10.53%). CONCLUSIONS: The outpatient prescription medication is basically reasonable in our hospital, yet the irrational problems still deserve attention, and more departments need to cooperate to improve the quality of prescriptions. [ABSTRACT FROM AUTHOR]

[ Objective] The present paper aimed to clarify the differences of microbial community structure and diversity under different coffee wastes aud their key driving factors, and reveal the regulatory mechanism of soil microbial community under the decomposition of coffee wastes, which is conducive to providing theoretical basis for maintaining farmlaud soil health in tropical region. [Method] The study was conducted in Wanning city, Hainan province. A randomized block design was adopted with four mulching treatments, including uncovered coffee waste ( C), covered coffee litter ( L), covered coffee peel ( P), and covered coffee litter and peel ( PL) at the same time. The soil physical and chemical properties, soil microbial abundance, richness, diversity and other indicators were measured to analyze the internal relationship between soil microclimate factors and soil microbial community structure, and explore the main factors driving the succession of soil bacterial and fungal communities under coffee waste mulching. [Result] The coffee peel and litter mulching significantly increased the content of soil available potassium and alkali hydrolyzable nitrogen by 11. 95% and 16. 19%, respectively, but did not affect other indicators. Coffee peel mulching significantly increased soil bacterial richness and diversity by 7. 75 % and 2. 79%, respectively, whereas coffee peel had little effect on bacterial community richness and diversity; Coffee peel covering significantly increased the relative abundance of Proteobacteria by 18. 79% and Firmicutes by 82. 52%, respectively, but significantly decreased the relative abundance of Cyanobacteria by 70. 17%; Coffee litter mulch significantly increased the relative abundance of Firmicutes by 59. 05%, but significantly decreased the relative abundance of Cyanobacteria by 73. 32%; The fungal community was not sensitive to coffee peel mulch, but coffee litter mulch significantly increased the relative abundance of Ascomycetes by 14. 80%, while reduced the relative abundance of Actinomycetes by 48. 38 % . The main functions of soil microbe did not change significantly, although the relative abundance of some functional microbe was altered by the coffee peel or litter mulching. [Conclusion] Coffee peel mulching significantly increases the soil available potassium content, alkali hydrolyzable nitrogen content, bacterial community diversity, bacterial community richness and the change of bacterial community structure. Coffee peel mulching cultivation is conducive to maintaining the relative stability of the structure and function of the soil microbial community in the coffee garden, thus promoting the health protection of the coffee garden soil and the sustainable development of the coffee planting industry. [ABSTRACT FROM AUTHOR]

Objective To investigate the incidence of retinopathy of prematurity (ROP) and analyse the associated risk factors of the severe ROP. Methods A total of 703 preterm infants, who met the ROP screening criteria in Department of Neonatology Maternal and Child Health Hospital of Changzhou and with integrated data from July 2011 to July 2014, were analysed in this study. ROP screening was done by ophthalmologist with binocular indirect ophthalmoscope at 4 weeks after birth or chronological age of 32 weeks. According to the screening results they were decided to be treated or not. Data were collected and compared for the severity of ROP in children with different gestational ages and birth weights. Results The detection rate of mild ROP was 5.26 % and severe ROP was 0.85%. Birth gestational age < 28 weeks and birth weight 500- 1 000 g of the children, the risk of severe ROP was significantly increased. The differences were statistically significant (P < 0.05). Conclusion Gestational age and birth weight are risk factors for severe ROP. Early detection and treatment can prevent the blindness of ROP. [ABSTRACT FROM AUTHOR]

目的探讨趋化素样因子样MRVEL跨膜结构域超家族成员3(CMTM3)在结直肠癌发生、发展中的作用。方法采用免疫组化法检测104例份结直肠癌组织及其配对癌旁正常组织中CMTM3表达,分析结直肠癌组织中CMTM3表达与患者临床病理参数的关系。结果 CMTM3在结直肠癌组织及癌旁正常组织中的阳性表达率分别为15.4%(16/104)、100%(104/104),二者比较P<0.01。结直肠癌组织CMTM3阳性表达与肿瘤直径、组织分化程度、NCCN分期及肿瘤类型有关(P均<0.05),与患者性别、年龄、肿瘤部位、淋巴结转移、合并肠道多发息肉及便血无关(P均>0.05)。结论结直肠癌组织中CMTM3表达降低,其表达降低可促进结直肠癌的发生、发展。 [ABSTRACT FROM AUTHOR]

Objective: To analyze the relationship between serum interleukin-18 (IL-18), interleukin-23 (IL-23), Interleukin-33 (IL-33) and intestinal flora and clinical outcome in patients with severe pneumonia (SP). Methods: 90 patients with SP who were admitted to Jinan People’s Hospital from December 2019 to June 2022 were selected as the study group.Another 90 patients with common pneumonia who were admitted in the same period were taken as the control group.The serum IL-18, IL-23, IL-33 and intestinal microflora in the two groups were compared, and the relationship between them was analyzed by Pearson correlation. In addition, the patients in the study group were divided into improvement group with 60 cases and deterioration group with 30 cases according to the difference of clinical outcome after treatment.The influencing factors of clinical outcome of patients with SP were analyzed by multivariate Logistic regression. Results: The levels of serum IL-18, IL-23 and IL-33 in the study group were higher than those in the control group (all P＜0.05).The contents of Escherichia coli and Enterococcus in the study group were higher than those in the control group, while the contents of Bacteroides, Bifidobacterium and Lactobacillus were lower than those in the control group (all P＜0.05). Pearson correlation analysis showed that serum IL-18, IL-23 and IL-33 were positively correlated with the contents of Escherichia coli and Enterococcus, but negatively correlated with the contents of Bacteroides, Bifidobacterium and Lactobacillus (all P＜0.05). The age and the levels of serum IL-18,IL-23 and IL-33 in the improvement group were lower than those in the deterioration group, and the proportion of mechanical ventilation and long-term bed rest were lower than those in the deterioration group (all P＜0.05). Multivariate Logistic regression analysis showed that older age, mechanical ventilation, long-term bed rest and high levels of serum IL-18, IL-23 and IL-33 were risk factors for the deterioration of patients with SP after treatment (all P＜0.05). Conclusion: Serum IL-18, IL-23 and IL-33 of patients with SP are closely related to intestinal flora, and with the increase of the above three serum indicators, the clinical outcome of patients is worse. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the clinical efficacy of silibinin combined with entecavir in treatment of liver cirrhosis of hepatitis B. METHODS: 72 patients with liver cirrhosis of hepatitis B admitted into Ningxia People’s Hospital from Jan. 2016 to Jan. 2017 were selected and divided into observation group and control group via random number table, with 36 cases in each. The control group was treated with entecavir, while the observation group was given silibinin based on the control group. The liver function indicators, liver fibrosis indicators and incidence of adverse drug reactions of two groups were observed. RESULTS: After treatment, levels of alanine aminotransferase, prothrombin time, serum total bilirubin and albumin ol two groups were better than those of before treatment, and the observation group was better than the control group, with statistical significance (P <0.05). After treatment, the hyaluronic acid, type IV procollagen and type HI procollagen levels were better than those of before treatment, and the observation group was better than the control group, with statistical significance(P <0. 05). The incidence of adverse drug reactions of observation group was 11. 12% (4/36), significantly lower than that of control group [30.58% (1 1/36) ], with statistical significance(P <0. 05). CONCLUSIONS: The efficacy of silibinin combined with entecavir in treatment of liver cirrhosis of hepatitis B is remarkable, which can effectively improve levels of liver function indicators and liver fibrosis indicators with fewer adverse drug reactions. [ABSTRACT FROM AUTHOR]

Objective To investigate the differences in bone metabolism and the influencing factors in Kazak and Han male patients with psoriasis vulgaris in Xinjiang. Methods The study group were 112 cases of Kazak male patients diagnosed in with psoriasis vulgaris selected during January 2012 to October 2017 in Department of Dermatology, Xinjiang Production and Construction Corps Hospital. Meanwhile, according to age and weight, 112 cases of Han nationality male patients with psoriasis who had been examined in our department during the same period were selected as the control group. Results For the serum concentrations of Ca, ALP, GLU and 25-OH-D3 in the two groups, the Kazakh group had significantly higher levels of serum Ca, ALP and 25-OH-D3 than the Han group, and the differences were statistically significant（P<0.05）. For the comparison of lumbar spine, femoral neck and ward’s triangle bone mineral density, bone mineral density of Kazak male psoriasis group was slightly higher than that of the Han group, the differences for L1, L3 and L4 were not statistically significant（P>0.05）, but there were significant differences in L2, femoral neck and ward triangle BMD（P<0.05）. Conclusion The bone metabolism level of Kazak nationality psoriasis patients was significantly higher than that of Han male psoriasis patients. [ABSTRACT FROM AUTHOR]

Objective Systematically summarize the research progress of clinical trials of gastric cancer oncology drugs and the overview of marketed drugs in China from 2012 to 2021, providing data and decision-making evidence for relevant departments. Methods Based on the registration database of the drug clinical trial registration and information disclosure platform of Food and Drug Administration of China and the data query system of domestic and imported drugs, the information on gastric cancer drug clinical trials, investigational drugs and marketed drugs from January 1, 2012 to December 31, 2021 was analyzed, and the differences between Chinese and foreign enterprises in terms of trial scope, trial phase, treatment lines and drug type, effect and mechanism studies were compared. Results A total of 114 drug clinical trials related to gastric tumor were registered in China from 2012 to 2021, accounting for 3.7% (114/3 041) of all anticancer drug clinical trials in the same period, the registration number showed a significant growth rate after 2016 and reached its peak with 32 trials in 2020. Among them, 85 (74.6%, 85/114) trials were initiated by Chinese pharmaceutical enterprise. Compared with foreign pharmaceutical enterprise, Chinese pharmaceutical enterprise had higher rates of phase I trials (35.3% vs 6.9%, P=0.001), but the rate of international multicenter trials (11.9% vs 67.9%, P<0.001) was relatively low. There were 76 different drugs involved in relevant clinical trials, of which 65 (85.5%) were targeted drugs. For targeted drugs, HER2 is the most common one (14 types), followed by PD-1 and multi-target VEGER. In the past ten years, 3 of 4 marketed drugs for gastric cancer treatment were domestic and included in the national medical insurance directory. Conclusions From 2012 to 2021, China has made some progress in drug research and development for gastric carcinoma. However, compared with the serious disease burden, it is still insufficient. Targeted strengthening of research and development of investment in many aspects of gastric cancer drugs, such as new target discovery, matured target excavating, combination drug development and early line therapy promotion, is the key work in the future, especially for domestic companies. [ABSTRACT FROM AUTHOR]

目的 观察侧脑室注入网膜素（omentin）对小鼠摄食量及其下丘脑组织神经肽 Y（NPY）、刺鼠相关蛋白（AGRP）、促肾上腺皮质激素释放激素（CRH）、阿片-促黑素细胞皮质素原（POMC）mRNA表达的影响。方法 20只昆明小鼠随机分为实验组及对照组,两组侧脑室置管并保留72 h后分别于侧脑室注射omentin及生理盐水（1.2μg/kg）,观察注射后24 h小鼠摄食量有无变化,RT-PCR检测两组小鼠下丘脑组织AGRP、NPY、CRH及POMC mRNA。结果实验组、对照组摄食量分别为（4.63±0.17）、（4.51±0.12）g,两组比较,P〉0.05。实验组、对照组NPY mRNA相对表达量分别为0.47±0.24、0.48±0.29,AGRP mRNA分别为24.31±6.21、22.01±7.14,POMC mRNA分别为0.92±0.48、0.83±0.16,CRH mRNA分别为0.93±0.45、0.69±0.12,两组CRH mRNA表达量比较,P〈0.05。结论侧脑室注入omentin对小鼠摄食量及下丘脑组织NPY、AGRP、POMC mRNA表达无影响,但CRH mRNA表达增加。 [ABSTRACT FROM AUTHOR]

Objective To investigate the difference of bone metabolism and its influential factors in patients with psoriasis vulgaris． Methods A total of 229 patients with psoriasis vulgaris were identified as the study group． At the same time，according to age and sex，229 cases of non-psoriatic healthy people who were conducted physical examination in physical examination center of our hospital were as the control group． Ｒesults In males，the serum levels of Ca and P in the psoriasis vulgaris group were lower than those in the non-psoriasis vulgaris group． The levels of Mg，ALP，TG，and LDL-C in male and female psoriasis groups were higher than those of non-psoriasis vulgaris group． The serum 25-OH-D3 in psoriatic group was higher than that in non-psoriasis vulgaris group． BMD of the lumbar spine in male and female and non-psoriasis vulgaris group was slightly higher than that in psoriatic group． BMD of L1 ，L2 ，and the lumbar spine in male psoriasis group was lower than that in female psoriasis group，and the mean of lumbar spine was statistically significant． BMD of the right femoral neck and Ward triangle in psoriasis group was slightly higher than that of the left side． According to the psoriasis disease course，osteopenia and osteoporosis mostly occurred in 10 － 15 years and 5 － 10 years ( 28. 319% and 27. 876%，respectively) ，followed in 15 － 20 years group ( 22. 566%) ． The normal proportion of BMD in male group was higher than that in female group ( 8. 850% in female group and 38. 496% in male group) ． In the same group，the proportion of osteopenia and osteoporosis or fracture in males accounted for 44. 585%，and in female group accounted for 72. 222%，which was significantly higher than in male group． In the female group，there were 2 cases of fractures ( 2. 778%) in 15 － 20 years group and over 20 years group． In male psoriasis vulgaris over 20 years group，1 case of bone fracture occurred ( 0. 637%) ． Conclusion Bone metabolism level in patients with psoriasis vulgaris is significantly lower than that in normal controls． Education of osteoporosis prevention should begin in the early onset of psoriasis. [ABSTRACT FROM AUTHOR]

Objective To observe the clinical value of antibody concentration of serum rheumatoid factor （Rheumatoid arthritis， factor，RF） and anti cyclic citrullinated peptide （Anti-cyclic citrullinated peptide， anti -CCP） in the diagnosis and treatment of patients with rheumatoid arthritis （Rheumatoid arthritis， RA）. Methods From April 2010 May -2015，we selected total of 58 cases of RA patients， and 54 cases of immune system diseases during the same period，55 cases of healthy people. Enzyme-linked immunosorbent assay was used for the detection of serum anti -CCP adsorption， immune scattering turbidimetry was used for the detection of serum RF. Then we observed the difference in the above indexes in three groups， and made ROC curve according to the two antibodies concentration and disease category to determine the critical limit of serum ant-CCP and RF in diagnosis of RA， and calculated the sensitivity， specificity， positive predictive value， negative predictive value， conformity of serum anti -CCP and RF in diagnosis of RA. Results In RA group， the concentration of RF was（59.34 + 10.34）U/ml， the positive rate was 84.48%，the concentration of antiCCP（59.34 + 10.34）U/ml， the positive rate was 81.03%; the concentration of RF in non RA group（14.32 +4.51）U/ml， the positive rate was 16.67%，the concentration of anti CCP（14.32 + 4.51）U/ml， the positive rate was 5.56%; health the control group the concentration of RF（6.78 + 2.12）U/ml， the positive rate was 1.82%，the concentration of anti CCP（2.35 + 1.06）U/ml， the positive rate was 0，RA group of anti CCP concentration and positive rate in RF， significantly higher than the other two groups（P＜0.05）.ROC curve of serum RF area under 0.742，sensitivity 62.3%，specificity 78.4%，accuracy 70.1%; ROC curve of serum anti CCP in the area under 0.813，sensitivity 72.5%，specificity 85.3%，accuracy 75.6%；RF combined with anti CCP area under the curve was 0.916，sensitivity 89.4%，specificity 95.6%，accuracy 87.4% RF combined with anti CCP， in the above indicators were significantly better than that of pure RF， anti CCP single index test results（P＜0.05）.At the end of treatment， the serum RF concentration was（23.53 +.P＜0.05）U/ml， serum anti CCP concentration was（7.83 + / - 3.45）U/ml， the end of treatment of the two indicators were significantly lower than before treatment， the difference was statistically significant. Conclusion Serum RF and anti -CCP antibody have high clinical diagnostic value in patients with RA， and also have some clinical significance for prognosis evaluation. [ABSTRACT FROM AUTHOR]

Objective To explore the relationship between insulin resistance, glucose and lipid metabolism related molecules and colorectal polyps. Methods A total of 262 healthy people who underwent colonoscopy in Shandong cancer hospital from June 2019 to September 2020 were selected. The levels of serum vascular cell adhesion molecule-1 (VCAM-1), fibroblast growth factor 19 (FGF19), insulin like growth factor (IGF-1), fasting blood glucose and fasting blood insulin were detected by enzyme-linked immunosorbent assay (ELISA). Insulin resistance index (HOMA-IR) was calculated, and the influencing factors of occurrence, pathological type, size and number of polyps were analyzed. Results Among 262 cases, 116 cases were polyp free, 113 cases were adenomatous polyp and 33 cases were inflammatory polyp. HOMA-IR, VCAM-1 and FGF19 in polyp group were 2.904±1.754, (334.415±139.573) ng/ ml and (135.865± 98.470) pg/ ml, respectively, which were higher than 2. 369 ± 1. 306, (302.480± 99. 946) ng/ ml and (110.694±76.044) ng/ ml in non-polyp group, respectively (P < 0.05). Multivariate Logistic regression analysis showed that the gender (OR =4.269, 95%CI: 1.963-9.405) and FGF19 (77.0-131.4 pg/ ml: OR = 2.385, 95%CI: 1.155-4.926) were independent factors of colorectal polyps. The gender (OR =3.799, 95% CI: 1.650-8.748) and FGF19 (77.0-131.4 pg/ ml: OR = 2.290, 95%CI: 1.072-4.891) were independent factors of colorectal adenomatous polyps. The gender(OR = 6.725, 95% CI: 1.853-24.410) and fasting plasma glucose (=6.5 mmol/ L: OR =0.047, 95%CI: 0.009-0.245) were independent factors of colorectal inflammatory polyps. The gender (OR = 3.539, 95% CI: 1.293-9.689) was an independent factor for the occurrence of single polyp. The gender (OR =5.063, 95% CI: 2.048-12.515), FGF19 (77.0-131.4 pg/ ml: OR =2.502, 95%CI: 1.102-5.681), fasting plasma glucose (=6.5 mmol/ L: OR = 0.282, 95%CI: 0.095- 0.839) were independent factors of multiple polyps. The gender (OR =3.416, 95% CI: 1.134-10.289) and fasting insulin (=9.4 µU/ ml: OR = 9.480, 95% CI: 1.485-60.521) were independent risk factors for colorectal polyps<0.5 cm. The gender (OR=3.151, 95%CI: 1.244-7.984) and fasting plasma glucose (=6.5 mmol/ L: OR =0.310, 95%CI: 0.102-0.941) were independent risk factors for colorectal polyps with the size of 0.5-0.9 cm. The gender (OR =22.649, 95%CI: 4.154-123.485), age (55 to 64 years old: OR = 4.473, 95%CI: 1.070-18.704; =65 years old: OR = 5.815, 95%CI: 1.300-26.009), BMI (=28 kg/ m2: OR = 5.310, 95%CI: 1.224-23.032) and FGF19 (77.0-131.4 pg/ ml: OR =7.474, 95%CI: 1.903-29.351) were independent factors for colorectal polyps with size = 1.0 cm. Gender stratification analysis showed that FGF19 was an independent factor for the occurrence of male polyps (77.0-131.4 pg/ ml: OR =6.109, 95%CI: 1.688-22.104) and adenomas (77.0-131.4 pg/ ml: OR =6.401, 95%CI: 1.717-23.864). The age (55 to 64 years old: OR = 3.783, 95%CI: 1.052-13.611) and VCAM-1 (=352.8 ng/ ml: OR = 4.341, 95%CI: 1.142-16.493) were independent risk factors of female polyps. The age (55 to 64 years old: OR = 5.743, 95%CI: 1.205-27.362, =65 years old: OR =6.885, 95%CI: 1.143-41.467), VCAM-1 (=352.8 ng/ ml: OR =6.313, 95%CI: 1.415-28.159) and IGF-1 (=7.6 ng/ ml: OR = 5.621, 95%CI: 1.069-29.543) were independent factors of female adenoma. Conclusions The occurrences of colorectal polyps and adenomatous polyps are related to insulin resistance and glucose and lipid metabolism. Serum FGF19 is an independent influencing factor for the occurrence of colorectal polyps and adenomatous polyps, and is a potential serological diagnostic marker and therapeutic target for colorectal polyps and adenomatous polyps. [ABSTRACT FROM AUTHOR]

目的 观察胚胎停育患者绒毛组织中hsa-miR-2276-3p及整合素9(ITGA9)、血管内皮生长因子(VEGF)mRNA表达变化,并分析其相关性。方法 收集正常早孕及胚胎停育行人工流产患者各30例,采用实时荧光定量PCR法检测绒毛组织中的hsa-miR-2276-3p及ITGA9、VEGF mRNA,并行Pearson相关分析法分析其相关性。结果与正常早孕者比较,胚胎停育患者绒毛组织中hsa-miR-2276-3p表达增高,而ITGA9、VEGF mRNA均表达降低(P均<0. 05)。在胚胎停育患者绒毛组织中,has-miR-2276-3p与ITGA9、VEGF mRNA的表达均呈负相关(r分别为-0. 679、-0. 528,P均<0. 05)。结论 在胚胎停育患者绒毛组织中hsa-miR-2276-3p高表达而ITGA9、VEGF mRNA低表达,hsa-miR-2276-3p与ITGA9、VEGF mRNA表达呈负相关; hsa-miR-2276-3p可能负向调节ITGA9、VEGF表达,从而导致绒毛组织中血管生成减少,引起胚胎停育的发生。 [ABSTRACT FROM AUTHOR]

Objective To explore the latest progress of oncology drug clinical trials in China under COVID-19, as well as to provide decision-making evidence for related stakeholders. Research progress of oncology drug trials and approved cancer drugs in China in 2020 were systematically summarized and compared with 2019. Methods Information Disclosure Platform for Drug Clinical Studies and China Food and Drug Administration Query System for Domestic and Imported Drug were searched for registered clinical trials and approved oncology drugs, respectively. The trial scope, stage, drug type, effect and mechanism of domestic and global pharmaceutical enterprises were compared between 2019 and 2020. Results A total of 722 cancer drug trials registered in China in 2020, with an annual growth rate of 52.3%, accounting for 28.3% of all registered trials. Among them, 603 (83.5%) trials were initiated by domestic pharmaceutical enterprises, and 105 (14.5%) were international multicenter trials, phase I trials accounted for 44.5%. For all those trials, there were 458 cancer drug varieties, with an annual growth rate of 36.7%, and 361 (85.8%) were developed by domestic enterprises. Most of the investigational products were therapeutic innovative drugs (77.1%), major in tumor treatment (92.8%). In terms of mechanism, targeted drugs were the most popular, accounting for 76.6%, and programmed cell death-1 (PD-1) and epithelial growth factor receptor (EGFR) were the most common targets. In addition, there were 19 anticancer drugs from 17 companies approved in China in 2019, with 10 drugs from domestic companies. Lung cancer and breast cancer are the most common indications for both registered trials and marketed drugs. No statistically significant differences were found between 2020 and 2019 in terms of the distribution of trial sponsor, scope and stage, as well as the distribution of drug type, effect and mechanism (P>0.05). Conclusions During the Covid-19 epidemic period, clinical trials of oncology drugs in China progress smoothly and maintain a high growth rate. Series of innovative products obtained by domestic enterprises in 2020 is the main driving force of development of oncology drug clinical trials in China. [ABSTRACT FROM AUTHOR]

Rotation, scaling, translation (RST) and boundary micro deformation are the main factors that affect the matching results of vector graphics, and are also good criteria for evaluating shape description algorithms. In this paper, a vector graphics matching algorithm using multi-dimensional object segmentation ratio is proposed for the influencing factors of shape matching. This algorithm extracts shape characteristic by constructing feature lines for vector graphics. It can accurately describe the shape characteristic of the polygon features to measure the shape similarity between the graphics. The vector graphics of 2 000 real geographical entities are used as the standard library for matching experiment, and half of the graphics are randomly selected as matching graphics. The matching graphics are taken to RST transformation and boundary simplification operations with varying degrees. The transformed graphics are matched with the standard library. The matching results are compared with other vector graphics matching algorithms to test the shape retrieval effect of this algorithm. This experiment demonstrate that the proposed algorithm has higher matching accuracy, RST invariance and deformation robustness. Therefore, it can accurately identify the shape of vector graphics. [ABSTRACT FROM AUTHOR]

To investigate the effect of silencing hepatocyte growth factor receptor (c-Met) expression on the biological characteristics of HCT116 colon cancer cells.MethodsCellular model of c-Met transient transfection was established by using small interfering RNA (siRNA), the expression of c-Met in colon cancer cells was detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and western blot. The apoptosis assay, cell invasion assay, cell migration and other experiments were conducted to observe the effects of silencing c-Met on the biological characteristics of colon cancer cells.ResultsRT-qPCR results showed that the relative expression levels of c-Met mRNA in siRNA-Met group, blank control group and siRNA negative control (siRNA-NC) group were 0.32±0.26, 1.01±0.03 and 1.05±0.23, respectively, and the difference was statistically significant (P<0.05). Western blot analysis showed that the expression level of c-Met protein in the siRNA-Met group was 0.24±0.03, significantly lower than 1.23±0.06 in the blank control group and 1.18±0.11 in the siRNA-NC group (P<0.05). The cell counting kit-8 (CCK8) results showed that the 72-hour absorbance (A) values of the siRNA-Met group, blank control group and the siRNA-NC group were 1.13±0.05, 1.48±0.08 and 1.53±0.07, respectively, and the difference was statistically significant (P<0.01). Cell cycle results showed that the proportion of cells in G2/M phase was (14.65±1.41)% in siRNA-Met group, (5.07±0.70)% in blank control group and (5.63±0.71)% in siRNA-NC group, and the difference was statistically significant (P<0.05). The expression levels of cell cycle regulatory proteins Cdc25c and cyclin B1 in siRNA-Met group were significantly decreased. The apoptotic rate in siRNA-Met group was (5.85±0.35)%, significantly higher than (1.00±0.17)% in blank control group and (0.91±1.14)% in siRNA-NC group (P<0.05). The expression level of apoptosis-related protein Bcl-2 in the siRNA-Met group was significantly decreased while Bcl-2 associated X protein (BAX) expression level was significantly increased. The cell scratching result showed that the cell migration abilities of the siRNA-Met group, blank control group and the siRNA-NC group were (51.33±8.62)%, (100.00±3.72)% and (102.33±6.43)%, respectively, and the difference was statistically significant (P<0.05). The number of cell penetrating into the basement membrane of the siRNA-Met group, blank control group and the siRNA-NC group were 47.50±10.60, 100.00±5.33 and 102.50±10.61, respectively, and the difference was statistically significant (P<0.05). The expressions of invasion related proteins including MMP-2 and MMP-9 in siRNA-Met group were decreased significantly.Conclusionsc-Met plays an important role in maintaining the biological characteristics of colon cancer cells. Inhibition of c-Met may have important values in the treatment of colon cancer. [ABSTRACT FROM AUTHOR]

Objective: To investigate the risk factors of abdominal infection after pancreaticoduodenectomy and analyze its etiological characteristics and drug sensitivity test. Methods: The clinical data of 60 cases undergoing pancreaticoduodenectomy in the 967th Hospital of the PLA Joint Logistics Support Force from January 2016 to December 2018 were retrospectively analyzed, the risk factors for postoperative abdominal infection were analyzed. The distribution and drug resistance of pathogenic bacteria in patients with postoperative abdominal infection were analyzed according to the results of bacterial culture and drug sensitivity test. Results: Univariate analysis showed that intraoperative blood transfusion, pulmonary infection, biliary fistula and pancreatic fistula were all related to the occurrence of abdominal infection (P<0.05). Multivariate Logistic regression analysis showed that intraoperative blood transfusion greater than 1000 mL, pulmonary infection, biliary fistula and pancreatic fistula were risk factors for abdominal infection after pancreaticoduodenectomy (P<0.05). A total of 54 strains were cultured in the peritoneal drainage fluid of 20 patients with postoperative abdominal infection. Among them, 23 strains (42.59%), 28 strains (51.85%) and 3 strains (5.56%) were Gram-positive bacteria. The main Gram-positive bacteria (Enterococcus faecalis, Staphylococcus epidermidis) were less sensitive to ciprofloxacin and erythromycin, all were below 17.00%, the sensitivity rates to linezolid and vancomycin were 100.00%. The main Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae) less sensitive to ceftazidime, cefazolin and piperacillin, all were below or equal to 25.00%, the susceptibility rates to gentamicin and imipenem were higher, all were higher or equal to 80.00%. Conclusion: Excessive blood transfusion during operation and occurrence of pulmonary infection, biliary fistula and pancreatic fistula are risk factors for abdominal infection after pancreaticoduodenectomy. Antibiotics should be use targetedly in clinic according to the results of drug sensitivity test. [ABSTRACT FROM AUTHOR]