Search

AIM: This study aimed to explore the induction of ferroptosis in non-small-cell lung cancer （NSCLC） cells by tubeimoside II （TBMS II） and to elucidate the underlying molecular mechanisms. METHODS: H460 NSCLC cells were cultured in vitro. Cell survival rates were assessed by using MTT assays, and doses of TBMS II resulting in below 50% survival were selected for further experimentation. Cell migration was evaluated using Transwell assays and the effects of TBMS II on H460 cell proliferation were assessed by colony formation assays. Flow cytometry and fluorescence microscopy were used to assess changes in lipid peroxidation （lipid ROS）, and the levels of GSH, T-AOC, MDA, and Fe2+ were measured using commercial kits. Protein levels of GPX4, SLC7A11, FTH1, NCOA4, P62, and LC3 were examined using Western blot. Changes in mitochondrial structure were detected by transmission electron microscopy, and immunofluorescence was used to assess LC3 co-localization of FTH1 and NCOA4, as well as co-localization of LC3 and NCOA4 with lysosomes. RESULTS: Compared with the control group, TBMS II dose-dependently reduced H460 cell viability, migration, and clone formation, accompanied by the appearance of vacuoles within the cells. TBMS II treatment also led to decreased GSH and T-AOC levels, while increasing the cellular contents of MDA, indicating oxidative stress. Additionally, there was a decrease in the expression of the antioxidant proteins SLC7A11 and GPX4 in the cells, while lipid ROS and Fe2+ levels were increased in proportion to the TBMS II concentration. The ferroptosis inhibitor ferrostatin-1 reversed cell death caused by TBMS II, suggesting ferroptosis induction. Furthermore, increasing the TBMS II concentration resulted in an upregulation of the autophagy marker proteins LC3 II/LC3 I and P62, indicative of increased autophagy. TBMS II also affected mitochondrial morphology in the cells, as seen in reduced mitochondrial fluorescence intensity. Protein expression of NCOA4 increased with higher TBMS II concentrations, while that of FTH1 decreased. Co-localization of LC3 II with FTH1 and NCOA4, as well as the lysosomal association of LC3 II and FTH1, also increased in a dosedependent manner. CONCLUSION: TBMS II induces ferritinophagy in H460 cells, leading to decreased cell viability and increased ferroptosis. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Hydrobiology is the property of Editorial Department of Journal of Hydrobiology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

With the development of emergency and critical care medicine, the patients with prolonged disorders of consciousness (pDOC) after brain injury are increasing year by year. Accurate assessment of patients' level of consciousness has become an important part of diagnosis and treatment. As an event-related potential (ERP) independent of patients' active attention, mismatch negativity (MMN) has a broad application prospect in the field of consciousness disorders. Based on existing research about pDOC and MMN, the classification and diagnosis of pDOC, and generation mechanism, result interpretation, clinical application of MMN. The particularity and importance of MMN in pDOC were highlighted. It provides reference for the follow-up basic research and clinical diagnosis and treatment of MMN in pDOC. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of High Pressure Physics is the property of Chinese Journal of High Pressure Physics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Mechanics / Gongcheng Lixue is the property of Engineering Mechanics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Data Acquisition & Processing / Shu Ju Cai Ji Yu Chu Li is the property of Editorial Department of Journal of Nanjing University of Aeronautics & Astronautics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)