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Copyright of Journal of Harbin University of Science & Technology is the property of Journal of Harbin University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Preliminary studies have confirmed that deep seawater not only affects the tolerance and immunity of mice, but also has an important regulatory effect on wound repair. OBJECTIVE: To explore the effect of deep seawater on the PI3K/Akt pathway in the process of wound repair in diabetic mice. METHODS: Forty-eight Kunming mice were enrolled to establish a diabetes model. A 1 cm2 wound was made on the back after successful modeling, and all the mouse models were randomized into four groups for corresponding treatments, including drinking purified water (control), tap water, deep seawater and deep seawater+LY294002, with 12 rats in each group. On the 3rd, 7th, and 10th days after wound modeling, the wound healing was observed, and western blot was used to detect the expression levels of vascular endothelial growth factor, basic fibroblast growth factor, hypoxia-inducible factor 1α, p-PI3K, p-Akt, PI3K and Akt in the wound tissue. qRT-PCR and western blot were used to detect the expression levels of collagen type I, collagen type III, interleukin-1β, tumor necrosis factor-α and transforming growth factor-β1 mRNA. On the 3rd and 10th days after wound modeling, hematoxylin-eosin staining was used to observe the morphological changes of the wound. The study protocol was approved by the Animal Ethic Committee of Kunming Institute of Zoology, Chinese Academy of Science with an approval No. KPRC-2017091. RESULTS AND CONCLUSION: The wound healing rate of the deep seawater group was higher than that of the control group and the tap water group (P < 0.01), while there was no significant difference in the wound healing rate between the tap water group and deep seawater+LY294002 group (P > 0.05). Hematoxylineosin staining showed that there were a large number of inflammatory cells infiltrated and a few fibroblasts on the wound surface in the control group, tap water group, and deep seawater+LY294002 group on the 3rd day, and granulation tissue, capillaries and fibroblasts were visible on the 10th day. In the deep seawater group, fibroblasts, capillaries and a few inflammatory cells were seen on the 3rd day, and new granulation tissue, more collagen fibers and a few capillaries were seen on the 10th day. Western blot analysis showed that the expressions of vascular endothelial growth factor, basic fibroblast growth factor, hypoxia-inducible factor 1α, p-PI3K and p-Akt in the deep seawater group were higher than those in the control group and the tap water group on each observational time point (P < 0.01). qRT-PCR and western blot results showed that the expressions of collagen type I, collagen type III and transforming growth factor-β1 in the deep seawater group were higher than those in the control group and deep seawater+LY294002 group on each observational time point (P < 0.05 or P < 0.01), while the expression levels of interleukin-1β and tumor necrosis factor-α were lower than those in the control group and deep seawater+LY294002 group (P < 0.05 or P < 0.01). To conclude, deep seawater can promote wound healing in diabetic mice via activating the PI3K/Akt pathway. [ABSTRACT FROM AUTHOR]

Objective　To explore the changing characteristics of fecal bacteria in patients with malignant obstructive jaundice. Methods　From January 2019 to May 2019，87 cases with malignant obstructive jaundice were taken as case bank and screened by inclusion and exclusion criteria. After that，10 patients and 10 healthy relatives of patients were selected， age， sex and BMI were recorded， and faeces were sent for 16srDNA copy number. Results （1）The fecal bacteria abundance and diversity index of the experimental group were lower than that of the control group， but there was no statistical significance （P < 0.05）. However， when the data of fecal bacteria abundance were presented in the Heatmap， the intuitive difference was more obvious. （2） The data of the two groups were subdivided by bacteria genus， and it was found that the composition of fecal bacteria genus in the experimental group changed fundamentally compared with that in the control group （ P < 0.05） . Among them， Escherichia-Shigella，Streptococcus，Veillonella，Enterococcus，Akkermansia increased significantly， while the bacteria genera of wiesia and clostridium declined significantly. Whether the intestinal flora can be rebalanced may affect the therapeutic effect. Conclusion　The fecal flora structure of patients with malignant obstructive jaundice is significantly different from that of normal control group， and the dominant flora genus has been fundamentally changed. [ABSTRACT FROM AUTHOR]

BACKGROUND: Deep seawater has been shown to effectively alleviate the process of diabetes in mice induced by high fat diet through activating AMPK pathway, but there are few studies about the role of deep seawater in the wound healing of diabetes mellitus. OBJECTIVE: To explore the mechanism of deep seawater on the cutaneous wound healing in diabetic mice. METHODS: Forty-eight healthy male Kunming mice (provide by Laboratory Animal Center of Kunming Medical University) were selected and were given intraperitoneal injection of streptozotocin to establish diabetic model. After 14 days of incubation, the back would model was established, and the mouse models were randomly divided into four groups: control group (purified water), tap water group, deep seawater group and inhibitor group (deep seawater + venous injection of Wnt/β-catenin inhibitor, XAV-939). Subsequently, the blood glucose and body mass of model mice were observed at 3, 7 and 10 days after wound modeling, and the healing of the wound was observed. The morphological changes of the wound were evaluated. The expression levels of β-catenin, GSK-3β and Rspo-3, malondialdehyde, superoxide dismutase and glutathione peroxidase in the serum were detected. The study was approved by the Laboratory Animal Ethics Committee of Kunming Institute of Zoology, China Academy of Sciences, approval No. KPRC-2017091. RESULTS AND CONCLUSION: (1) Deep seawater showed no significant effect on the body mass and blood glucose. (2) The wound healing rate in the deep seawater group was significantly higher than that in the tap water and control groups at different time points (all P < 0.01). (3) Western blot results showed that the expression levels of β-catenin and Rspo-3 were higher in the deep seawater group than the control and tap water groups, but GSK-3β was down-regulated. (4) Hematoxylin-eosin staining showed that compared with the control group, vascular endothelial cells, new capillaries and fibroblast cells proliferation in the deep seawater group were increased, but the number of inflammatory cells got opposite results. However, there was no significant difference between the deep seawater + XAV-939 group and control group (P > 0.05). The healing rate of wound at different time points in the deep seawater group was significantly higher than that in the tap water and control groups (all P < 0.01). (5) Compared with the control group, the expression levels of superoxide dismutase and glutathione peroxidase in serum in the deep seawater group were increased (P < 0.01 and P < 0.001), and malondialdehyde decreased (P < 0.05 and P < 0.01). There was no significant difference between deep seawater + XAV-939 and control groups (P > 0.05). (6) To conclude, deep seawater promotes cutaneous wound healing in diabetic mice via activating Wnt/β-catenin pathway. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oncology is the property of Chinese Journal of Oncology / Zhonghua Zhongliu Zazhi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)