Search

Objective To investigate the relationship of miRNA gene polymorphisms with blood pressure (BP) responses to the sodium and potassium diet intervention. Methods In 2004, we recruited 514 participants from 124 families in seven villages of Baoji, Shaanxi Province, China. All subjects were given a three-day normal diet, followed by a seven-day low-salt diet, a seven-day high-salt diet, and finally a seven-day high-salt and potassium supplementation. A total of 19 miRNA single nucleotide polymorphisms (SNPs) were selected for analysis. Results Throughout the sodium-potassium dietary intervention, the BP of the subjects fluctuated across all phases, showing a decrease during the low-salt period and an increase during the high-salt period, followed by a reduction in BP subsequent to potassium supplementation during the high-salt diet. MiR-210-3p SNP rs12364149 was significantly associated with systolic BP (SBP), diastolic BP (DBP) and mean arterial pressure (MAP) responses to low-salt diet. MiR-4638-3p SNP rs6601178 was significantly associated with SBP while miR-26b-3p SNP rs115254818 was significantly associated with MAP responses to low-salt intervention. In addition, miR-26b-3p SNP rs115254818 was significantly correlated with SBP, DBP and MAP responses to high-salt intervention. MiR-1307-5p SNPs rs11191676 and rs2292807 were associated with SBP and MAP responses to high-salt diet. MiR-4638-3p SNP rs6601178, miR- 210-3p SNP rs12364149, miR-382-5p SNP rs4906032 and rs4143957 were significantly associated with SBP response to high-salt diet. In addition, miR-26b-3p SNP rs115254818 was significantly associated with SBP, DBP and MAP responses to potassium supplementation. MiR-1307-5p SNPs rs11191676, rs2292807, and miR-19a-3p SNP rs4284505 were significantly associated with SBP responses to high-salt and potassium supplementation. Conclusion miRNA gene polymorphisms are associated with BP response to sodium and potassium, suggesting that miRNA genes may be involved in the pathophysiological process of salt sensitivity and potassium sensitivity. [ABSTRACT FROM AUTHOR]

目的 探讨基于整体责任制的临床护理路径(CNP)干预对 CT 增强检查患者心理状况及检查 配合度的影响. 方法 选取为2021年1月至2023年4月该院CT 增强检查患者88例,以随机数字表法按1∶ 1比例分为对照组和观察组,各44例. 对照组接受常规护理干预,观察组接受基于整体责任制的 CNP干预,比 较2组广泛性焦虑障碍量表(GAD-7), 9项患者健康问卷抑郁量表(PHQ-9)评分,以及检查配合度和满意度. 结果 2组干预前 GAD-7, PHQ-9评分比较,差异无统计学意义(P>0.05). 干预后,2组 GAD-7, PHQ-9评 分低于干预前,且观察组低于对照组,差异有统计学意义(P<0.05). 观察组检查配合度高于对照组,差异有统 计学意义(P<0.05). 观察组满意度为97.73%(43/44),高于对照组的81.82%(36/44),差异有统计学意义 (χ2=4.456,P=0.035). 结论 基于整体责任制的 CNP干预可改善 CT 增强检查患者不良心理,提高其检查 配合度及满意度. [ABSTRACT FROM AUTHOR]

Objective To explore the status quo of stress-related growth of caregivers of children with congenital pseudarthrosis of the tibia (CPT) and examine its possible influencing factors. Methods For this retrospective study, Caregivers of CPT children admitted into Hunan Children's Hospital from January to March 2023 were selected as study subjects. Simplified coping behavior questionnaire, social support rating scale, simplified Chinese version of post-traumatic growth rating scale and general profile questionnaire were utilized. Multiple linear regression and Pearson's correlation analyses were performed for examining the major influencing factors of caregiver stress-related growth in CPT children. Results Among a total of 161 questionnaires distributed, 155 were valid with an effective rate of 96.27%. Total stress-related growth score was (60.86±16.13). Pearson's correlation analysis indicated that all dimensions of stress-related growth and total scores of caregivers were correlated positively with positive coping styles (P<0.05). Besides self-transformation dimension, all dimensions of stress-related growth and total scores were correlated positively with social support style (P<0.05). According to multiple linear regression, children's age, caregiver occupation (whether employed or not), active coping and social support were the major influencing factors of stress-related growth of caregivers of CPT children (P<0.05, adjusted R²=0.443). Conclusions The stress-related growth of caregivers of CPT children is at a moderate level. Active coping, social support, children's age and caregiver occupation are the major factors affecting the stress-related growth level of caregivers of CPT children. Therefore, in clinical practices, attention should be paid to caregivers' coping styles and social support. The corresponding intervention measures may be formulated according to the above influencing factors to enhance their stress-related growth level and thus improve the outcomes of children. [ABSTRACT FROM AUTHOR]

The global population has entered a stage of ageing, and the incidence of frailty is increasing year by year. Frailty is not only associated with dysfunction of multiple systems, but also with mitochondrial dysfunction, chronic inflammation, oxidative stress and malnutrition. Among them, malnutrition is considered to be a key factor in its pathophysiological changes. As malnutrition is a modifiable risk factor for frailty, early nutritional interventions can be used to prevent frailty and improve the quality of life of the frailty elderly. [ABSTRACT FROM AUTHOR]

Objective To analyze the pesticriele; residues in vegetables sold in Hubei Province, and to evaluate; the pesticide; residue; risks. Methods 1 327 samples of 8 types of vegetables were; collected in Hubei Province; in 2022. 46 pesticide; residues were; detected and analyzed, and the; indc;x of foed safety method was used to assess risks. Results 35 were; ele;te;e;te;ei and 16 exceeded the; standard among the; 46 pesticides. The; highest detection rate; was cnoylmorpholinc; (53.09%) anel the; highest exceeding rate; was triazophos (22. 22%). 7 kinds of vegetables were; detected, anel 5 kinds of vegetables exceeded the; stanelarel among the; 8 kinels of vegetables. The; highest detention rate; was melon (65. 79%) anel the; highest exceeding rate; was bean (32. 53%). The; safety index of the; average; value; erf elete;e;te;el pesticide; rcsielucs was less than 1, anel the; safety index of the; maximum value; erf se;ve;n pesticide; residues, such as fipronil, triazophos was more; than 1. Conclusion The; overall risk of pesticide; residues in vegetables sold in Hubei Province; is relatively low in 2022, but some; vegetable; varieties have; a high rate; erf' exceeding the; stanelarel. The; maximum detected values erf pesticide; residues sucli as flufenitrile;, triazophos pose; certain safety risks. [ABSTRACT FROM AUTHOR]

In recent years, an increasing number of researchers have attempted to link catalytic activity to the acidity and basicity of the oxygen vacancies in catalysts. A deeper understanding of the role played by oxygen vacancy defects in determining catalytic properties and charge carrier dynamics by combining Lewis acid-base active sites can provide valuable insights for the rational development of more efficient catalysts. Oxygen vacancies are the most common defect in transition metal oxides, and catalysts containing oxygen vacancies are closely related to how surface acidity and basicity affect the adsorption, reaction, and desorption processes of reactants, intermediates, and solid surface products. To this end, the location and the acid-base of oxygen vacancies have always played a central role in the history of multiphase catalysis, and the study of the construction of Lewis acid-base sites based on oxygen vacancy defects in catalysts is of great significance for the performance enhancement of various molecular activation and catalytic conversion reactions. The surface properties of multiphase catalysts will largely determine the electronic structure of the surface active sites. Therefore, this paper reviews the research on the oxygen vacancy-containing solid catalysts with Lewis acid-base sites, explores the effects of the corresponding active sites on the catalytic efficiency, stability and chemoselectivity of the catalysts, and summarizes their relevant applications in the field of photocatalysis. [ABSTRACT FROM AUTHOR]

[Objective]To isolate and screen the high-efficiency cellulose degrading bacteria from traditional Chinese medicine residue, and to determine the cellulase activity in order to improve the added value of traditional Chinese medicine residue. [Method]The decomposition products of traditional Chinese medicine residues and soil samples piled up with drug residues for a long time were enriched and cultured in Hutchison medium, and the strains with drug residues as the only carbon source were isolated and purified. The obtained strains were primary screened by Congo red plate method, secondary screening by determine the strain’ s filter paper activity, exo-β-glucanase activity, endo-βglucanase activity and glucosidase activity. The strains were identified by morphology, physiology and biochemistry, 16S rDNA sequence analysis. [Result]One cellulose degrading strain YZ25 with good cellulase production was screened, with FPA enzyme activity of 25. 073 U/ mL, CMC enzyme activity of 24. 978 U/ mL, CBH enzyme activity of 58. 452 U/ mL, and CB enzyme activity of 37. 202 U/ mL. The strain YZ25 was identified as Bacillus subtilis. [Conclusion] This study enriches the resource pool of cellulose degrading strains from drug residues and has a good application prospect, which has practical significance for the research and development of functional biological organic fertilizer from traditional Chinese medicine residue. [ABSTRACT FROM AUTHOR]

To investigate the potential targets and related mechanisms of micheliolide (MCL) in alleviating gouty arthritis (GA). The network pharmacology technology was used to retrieve MCL and GA related targets, screen their intersection targets, construct protein interaction maps and screen core targets. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was conducted, the ″MCL-target-pathway-GA″ network diagram was drawn, and molecular docking verification of MCL and core target was conducted. Results A total of 778 MCL related targets, 351 GA related targets and 58 intersection targets were screened out. Twenty-four targets were predicted to be the core targets of MCL inalleviating GA, such as TNF, IL-1B (IL-1β) and so on. GO analysis confirmed 294 items related to biological process (BP), 38 items related to cellular components (CC) and 57 items related to molecular function (MF). KEGG analysis revealed 105 signaling pathways, including inflammation, metabolism, infection and tumor. The molecular docking results showed that MCL binds tightly to the core target of TNF and IL-1B (IL-1β) by hydrogen bonding, and the MCL binds most tightly to the core target of IL-1B (IL-1β). The core targets and related biological processes and signaling pathways of MCL alleviating GA were preliminarily predicted, and molecular docking verification was carried out, providing ideas for further in vivo and in vitro experiments. [ABSTRACT FROM AUTHOR]

Objective Corin, a transmembrane serine protease that can cleave atrial natriuretic peptide precursor (pro-ANP) into atrial natriuretic peptide with smaller bioactive molecules, participates in the pathophysiologicalprocess of hypertension and cardiac hypertrophy. The purpose of this study was to explore the relationship of Corin gene variation with blood pressure responses to sodium and potassium dietary interventions. Methods In 2004, we recruited 514 participants from 124 families in 7 villages of Baoji, Shaanxi Province, China. All the subjects received a 3-day normal diet, a 7-day low-salt diet, a 7-day high-salt diet, and finally a 7-day high-salt and potassium supplementation. Fifteen single nucleotide polymorphisms (SNPs) of Corin gene were selected for final analysis. Results SNPs rs12509275 were significantly associated with diastolic blood pressure (DBP) response to low-salt diet, while rs3749584 was associated with pulse pressure (PP) response to low-salt diet.SNP rs3749584 and rs10517195 were significantly associated with PP response to high-salt diet. In addition,rs17654278 were significantly associated with systolic blood pressure (SBP) response to high-salt and potassium supplementation, rs2271037 was significantly correlated with DBP responses to high-salt and potassium supplementation, and rs4695253, rs12509275, rs2351783, rs36090894 were significantly associated with PP response to high-salt and potassium supplementation. Conclusion Corin gene polymorphisms were associated with blood pressure response to sodium and potassium, suggesting that Corin gene may be involved in pathophysiological process of salt sensitivity and potassium sensitivity. [ABSTRACT FROM AUTHOR]

A nonlinear switching control method was proposed based on an improved quasilinear autoregressive with exogenous inputs (quasi-ARX) radial basis function (RBF) network model and the support vector regression (SVR) algorithm. The RBF network was chosen as the nonlinear part of the improved quasi-ARX prediction model. The proposed controller design method was divided into 3 steps: firstly, the nonlinear parameters of the model were determined with the clustering method; secondly, the linear SVR algorithm was used to solve the robustness problem of the control system; thirdly, the switching criterion function was given based on the control error, and the control sequence were determined according to the switching law. Finally, a numerical example was given to verify the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

To research the effect of Chinese (AOHUA AQ-100 type) endoscope on the diagnosis of heterotopic gastric mucosa in the upper esophagus (HGMUE) under anesthesia.Methods The patients who underwent endoscopy in the endoscopy center of a hospital from January 2018 to March 2020 were selected as the research objects and randomly divided into the experimental group and the control group.The AOHUA AQ-100 type gastroscope (AQ-100-type medical endoscopic image processor and AQL-100-type xenon cold light source) was used in the experimental group.while the Olympus gastroscope (GIF-Q260J-type) was used in the control group.A total of 2 000 cases were examined in each group respectively.The patients were divided into the painless test group and the general test group for examination on a voluntary basis.In each group,moderate air injection observation was started at about 15 cm from the incisor,and then full air injection was started at about 25 cm from the incisor while slowly withdrawing the gastroscope (from about 25cm from the incisor to about 15cm from the incisor for more than 30 seconds),and careful observation was carried out.When the orange-red lesion with a clear boundary was observed,its position,size,shape and quantity were recorded,after considering it as HGMUE.Then,one to two pieces of mucosa were biopsied in the center of the suspected lesion and sent for pathological examination.The diagnosis was made according to the pathological results.Results In the experimental group,the total detection positive rate of HGMUE was 1.80% (36/2 000).Among them,the detection positive rate was about 1.35% (14/1 030) in the ordinary gastroscope group and 2.29% (22/970) in the painless gastroscope group.In the control group,the total detection positive rate of HGMUE was 2.00% (40/2 000).Among them,the detection positive rate was about 1.67% (13/780) in the ordinary gastroscope group and 2.21% (27/1 220) in the painless gastroscope group. In this study,there was no significant statistical difference in the total detection positive rate of HGMUE between the experimental group and the control group (P >0.05).There were no significant statistical differences about the positive rate in the painless gastroscope groups and the ordinary group between the experimental group and the control group too.Conclusion The application of the AOHUA AQ-100 medical gastroscope has the advantages of moderate price,clear image and good performance,which is helpful to promote the popularization of endoscopy in our country,especially in painless gastroscopy.It can improve the diagnosis rate of HGMUE. [ABSTRACT FROM AUTHOR]

目前,胶质瘤细胞培养技术及方法已经成熟,并已建立了多种胶质瘤细胞系,广泛应用于胶质瘤的基础实验研究, 包括放疗、化疗等方面的细胞学实验及各种模型的建立。既往研究多选用二维细胞培养技术,但其存在一些不足。三维细胞培养 技术的发展及广泛应用,让胶质瘤研究充满了前景。该文主要介绍三维细胞培养技术在胶质瘤细胞培养中的应用。 [ABSTRACT FROM AUTHOR]

A quasi-ARX multilayer learning network prediction model was established and applied to the adaptive control of nonlinear systems. The kernel of the model is an improved neuro-fuzzy network: one part is a 3-layer nonlinear network with an off-line training self-associative network, the other part is a 3-layer neuro-fuzzy network adjusted online. Accordingly, the parameters were classified and the corresponding estimation algorithms were given. Then, the controller design scheme was proposed based on the advantages of the macrostructure of the model. Simulation analysis verifies the effectiveness of the proposed model. [ABSTRACT FROM AUTHOR]

A kind of single-module repairable system with warning state was investigated， which can be expressed as a set of differential equations. By selecting the appropriate state space and the definition domain of the system operator， the equations were transformed into an abstract Cauchy problem in a Banach space. With the functional analysis method and linear operator semigroup theory， the eigenvalue 0 was proved to be the strictly dominant simple eigenvalue of the system. It shows the solution of the system is asymptotically stable. Then by studying the spectrum distribution of the main operator of the system， it was proved that the essential spectrum bound of the main operator of the system is negative. Finally， the variation of essential spectrum bound of the main operator under compact perturbation were discussed. The results demonstrate that the dynamic solution of the system is exponentially stable. [ABSTRACT FROM AUTHOR]

In order to meet the requirements of low-carbon economy,the reduction and utilization of global-warming gases have become the focus of attention.There are a large amount of toxic and harmful gases being emitted from the combustion of fossil fuels and syngas,as well as being released during mining activities.It is therefore necessary to trap and separate these gases for eliminating their environmental contamination,also increasing the feedstocks for industrial production.For avoiding the contamination and safety problems raised from the conventional alkali absorbents,the ionic liquids,namely “liquid molecular sieve”,have become green alternatives to absorb and separate different gases.Accordingly,the research progress about separating gases by pure ionic liquids is reviewed in this paper.The influencing factors including temperature,pressure,the properties of gas and ionic liquids,and the functional groups of ionic liquids,were analyzed.It is stated that the ionic liquids with alkali groups on cation and anion are promising CO2solvents and competent separator for removing CO2from some sparsely soluble gases.In order to separate CO2from strongly acidic SO2 and H2S,moderate basicity exhibits much better separating ability because strong alkali group could strongly bind with both SO2(or H2S) and CO2,leading to low SO2/CO2and H2S/CO2 selectivities.Ionic liquids with small molecular weights and compact structures usually perform better selectivity,and low temperature and pressure are favorable for increasing the separation.Additionally,combining experimental results and molecular dynamic simulation,the real selectivity of binary gases in ionic liquids was calculated based on Equations of State.It is concluded that feed gas ratio,ionic liquid contents,temperature and pressure are significant factors required to be adjusted to approach a great real gas selectivity. [ABSTRACT FROM AUTHOR]

Objective To investigate the endothelial dysfunction in pre-hypertension and its influencing factors. Methods A total of 373 youth were divided as the subjects into hypertension group (HBP group), pre-hypertension group (PHT group) and normal blood pressure group (NBP group). Endothelial function was assessed based on carotid intima-media thickness (IMT), brachial artery flow-mediated dilation (FMD) and brachial-ankle pulse wave velocity (baPWV). Results IMT and baPWV in PHT group were higher than those in NBP group (P<0.05), but did not reach the significant difference when compared with HBP group (P>0.05). Compared with HBP, the levels of FMD in PHT group significantly increased (P<0.05); however, no difference was observed in comparison with NBP group (P>0.05). In the early stage of hypertension, diastolic BP (β=－0.120, P<0.05) and body mass index (β=－0.115, P<0.05) were negatively correlated with FMD; diastolic BP (β=0.146, P<0.05), 2-hour glucose (β=0.147, P<0.05), high-density lipoprotein cholestrol (β=0.150, P<0.05), and waist-hip ratio (β=0.126, P<0.05) showed a positive correlation with IMT. baPWV was correlated with systolic BP (β=0.358, P<0.01), waist circumference (β=0.254, P<0.05), fasting glucose (β=0.155, P<0.05), postprandial 2h blood glucose (β=0.152, P<0.05), uric acid (β=0.206, P<0.05), and C-reactive protein (β=0.099, P<0.05). Conclusion Our study shows that endothelial dysfunction may exist in the pre-hypertensive young, and several cardiovascular risks contribute to its development in the early stage of hypertension. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of different dosage of icariin on the RANKL and Wnt3a expressions in the pressure side of periodontal tissue during orthodontic tooth movement (OTM). Methods: Twenty four male SD rats were randomly divided into four groups according to the different dosage of icariin as follows: physiological saline group (control group); 1 mg/kg icariin group; 3 mg/kg icariin group: 5 mg/kg icariin group (experience group). 50 g force was used to move the left maxillary first molar. Immunohistochemical staining technology was used to detect the expression of RANKL and Wnt3a in the pressure side of periodontal tissue. Results: Rats in the physiological saline group showed crude surfaces of the root of tooth and alveolar bone at the pressure area of the maxillary first molar and narrowed space of the periodontium, and bone resorption pits and osteoclasts could be observed. Rats in various dosage of icariin group displayed that the space of the periodontium tended to recover normally, and significantly reduced bone resorption pits could be noted. RANKL and Wnt3a expressed on both the physiological saline and icariin groups in the pressure zone of periodontal tissue. Compared with the saline group, the expression of RANKL was significantly decreased in the pressure side of periodontal tissue in the different dosage of icariin groups, and the expression of Wnt3a were significantly increased. Furthermore, the expression of RANKL was decreased gradually with the increase of dosage (P<0.05). And the expression of Wnt3a was increased gradually with the increase of dosage (P<0.05). Conclusions: Different dosage of icariin could reduce the expressions of RANKL and increase the expression of Wnt3a in the periodontal tissue remodeling during OTM. and its effect has certain correlation with its dosage. [ABSTRACT FROM AUTHOR]

Coalmine fire caused by coal spontaneous combustion is one of the main disasters in coalmines. The crossing-point temperature (CPT) method is an experimental technique to test the propensity of coal to spontaneous combustion and the characteristic parameters of temperature increase. A Temperature-Programmed System (TPS) was used to develop the oxidation tests of the bituminous coal under various flow and oxidation conditions for temperatures at low temperatures (such as the volume fraction of oxygen in air at inlet are 20.65%，17.80%，13.62%，10.49%，5.00% and 3.00% respectively). Based on the present analysis of temperature increase rate of coal，the temperature differences between oven and coal，and the characteristics of oxygen consumption，furthermore，considering the heat generation rate of coal during the procedure of the Temperature-Programmed tests，the four stages of coal oxidation at low temperature were identified: (I) initial heating with no reaction，(II) a endothermic period，(III) an unsteady exothermic period and (IV) a steady period. Moreover，according to the normalization of the energy conservation equation of the coal spontaneous combustion during the tests，the temperature differences of the oven and the coal samples were dominated by the parameters of ατ and (B-1) ατ separately at the stages I & IV. Also，there must be parameter B larger than 1 as the CPTs occur at stage III. The theoretical results remains the same as the experimental ones. [ABSTRACT FROM AUTHOR]

Using Yuanjia Village in Liquan County, Shaanxi Province, China as a case study, this paper uses qualitative research methods to analyze the place identity construction of rural in‐ coming tourism entrepreneurs from the perspective of everyday life. The results show that the in‐ coming tourism entrepreneurs in Yuanjia Village have a strong emotional attachment to rural pastoral life, which is expressed through the everyday life worlds of rural areas consisting of every‐ day livelihood, everyday communication and everyday concepts. In the everyday life worlds of rural areas, the incoming tourism entrepreneurs are continuously shaping the life imagination and identity cognition after escaping from urban modernity. With the increase of everyday inter‐ active practice in the destination of incoming tourism entrepreneurs, identity will also be gradual‐ ly transformed and endowed with placeness, further stimulating the positive behavior of individu‐ als to the destination. [ABSTRACT FROM AUTHOR]

王兰，博士研究生，副主任医师，长期从事消化内科临床、教学、科研工作， 熟练掌握了消化系统常见疾病的诊治和急、危重患者的抢救。尤其在老年消化系统疾病的 诊治、消化系统肿瘤的诊治方面积累了较丰富的经验。从事老年病及全科医学临床工作及 师资带教工作8年，擅长老年疾病的综合评估、多团队合作诊治，各种老年人危重急症的抢 救，老年肿瘤的综合治疗，老年衰弱患者的长期照顾和护理指导。在科研方面，对肝缺血再 灌注损伤及非酒精性脂肪性肝病的发病机制进行了深入研究。目前主持厅局级科研课题 3 项，已发表学术论文20篇，其中SCI收录4篇。中国老年学会医养结合分会委员，《肝博士》 杂志编委。 [ABSTRACT FROM AUTHOR]

目的　观察节律基因Ｐｅｒｉｏｄ1和Ｐｅｒｉｏｄ2在胃癌组织中的表达变化，并探讨其临床意义。方法　选择138 例胃癌患者，术中留取胃癌及癌旁组织，采用免疫组化法检测胃癌及癌旁组织中的Ｐｅｒｉｏｄ1和Ｐｅｒｉｏｄ2，实时荧光定量 ＰＣＲ法检测胃癌及癌旁组织中的Ｐｅｒｉｏｄ1和Ｐｅｒｉｏｄ2ｍＲＮＡ。结果　胃癌组织中Ｐｅｒｉｏｄ1和Ｐｅｒｉｏｄ2阳性表达率分别为 23.2％、31.2％，均低于癌旁组织中的44.9％、64.5％（Ｐ均＜0.01）；胃癌组织中Ｐｅｒｉｏｄ1ｍＲＮＡ和Ｐｅｒｉｏｄ2ｍＲＮＡ相对 表达量分别为1.05±0.10、1.09±0.12，均低于癌旁组织中的1.31±0.13、1.47±0.16（Ｐ均＜0.01）；Ｐｅｒｉｏｄ1ｍＲＮＡ表 达与胃癌组织分化程度、浸润深度、淋巴结转移和Ｌａｕｒｅｎ分型有关（Ｐ均＜0.05），Ｐｅｒｉｏｄ2ｍＲＮＡ表达与胃癌组织分化 程度、浸润深度、ＴＮＭ分期、淋巴结转移和Ｌａｕｒｅｎ分型均有关（Ｐ均＜0.05）；相关性分析显示，胃癌组织中Ｐｅｒｉｏｄ1与 Ｐｅｒｉｏｄ2表达呈正相关（ｒ＝0.285，Ｐ＜0.05），Ｐｅｒｉｏｄ1ｍＲＮＡ与Ｐｅｒｉｏｄ2ｍＲＮＡ表达呈正相关（ｒ＝0.379，Ｐ＜0.05）。结论 　胃癌组织中节律基因Ｐｅｒｉｏｄ1和Ｐｅｒｉｏｄ2呈低表达，可能在胃癌的发生、发展中起重要作用。 [ABSTRACT FROM AUTHOR]

[Objective] To select oat varieties with high aphid resistance, strong production performance and excellent comprehensive performance, to provide theoretical references for the healthy development of the oat industry in Inner Mongolia. [Methods] In Shaerqin farming-pastoral test site of Institute of Grassland Research of Chinese Academy of Agricultural Sciences in Tumed Left Banner, Hohhot, taking 17 oat varieties as test materials, the aphid resistance was identified by aphid ratio method. The aphid resistance grade of each variety was determined. And the agronomic traits and yield were used as indicators to compare the differences in production performance of the varieties. Bivariate correlation analysis and systematic cluster analysis were performed using agronomic parameters, yield, and aphid ratio as indicators. [Results] The aphid resistance of the test oat varieties was categorized into 6 grades. Baiyan No.7, Mengyan No.3, Qinghai 444, and Qingyin No.2 had high resistance. Qinghaitian oats and Qingyan No.1 showed medium resistance. Mengsiyan No.2, Lingxiu, Beile, Taiyangshen, and Yellow Oats had low resistance. Bayan No.1 and Bayan No.7 were low susceptible. Bayan No.5 was medium susceptible. Aiwo126, Yanwang and Furuizhi were highly susceptible. The plant height of Baiyan No.7 and Taiyangshen reached over 1.18 m, which was significantly higher than that of Bayan No.7, Lingxiu, Yanwang, Qingyin No.2, Aiwo 126, Furuizhi (P<0.05). Beile had the highest fresh grass yield (4.34 kg/m2), which was significantly higher (P<0.05) than other varieties except Bayan No.5 and Aiwo 126. Taiyangshen had the highest hay yield (1.69 kg/m2), which was significantly higher (P<0.05)than that of Bayan No.7 and Qingyin No.2. There was no significant difference (P>0.05)in the fresh-to-dry ratio of the 17 oat varieties. Aiwo 126 had the highest (2.98) fresh-to-dry ratio, while Qinghai 444 had the lowest (1.86). The results of cluster analysis showed that the aphid ratio, plant height and yield performance of different oat varieties varied. The comprehensive performance of class I oat varieties (Qinghaitian oats, Mengyan No.3, Qinghai 444, Baiyan No.7, Lingxiu, Qingyan No.1, Yellow Oats, Taiyangshen, Bayan No.1, Bayan No.5, Bayan No.7, and Mengsiyan No.2) was stronger. [Conclusion] Qinghaitian oats, Mengyan No.3, Qinghai 444, Baiyan No.7, Lingxiu, Qingyan No.1, Yellow Oats, Taiyangshen, Bayan No.1, Bayan No.5, Bayan No.7 and Mengsiyan No.2 had strong aphid resistance, good production performance, and excellent comprehensive performance.They were suitable for planting in Tumed Left Banner in Inner Mongolia. [ABSTRACT FROM AUTHOR]

The experiment was conducted to study the effect of composite plant extracts Piper sarmentosum Roxb. and guava leaf on growth performance and serum indexes of weaned piglets. A total of 200 21-day-old weaned piglets were selected and randomly divided into two groups, with five replicates in each group and 20 piglets in each replicate. Piglets in control group were fed with a basal diet, the piglets in experimental group were fed with the basal diet supplemented with composite plant extracts at 200 mg/kg. The experimental period was 21 d. The results showed that there was no significant difference on growth performance of piglets among the groups（P>0.05）. Compared with control group, the experimental group supplemented with the compound plant extract in the diet significantly decreased the serum cholesterol（CHOL） and low-density lipoprotein cholesterol（LDL-C） content of piglets（P<0.05）, glutathione peroxidase（GSH-Px）activity was significantly increased（P<0.05）, serum content of interleukin-4（IL-4）, interleukin-10（IL-10） and transforming growth factor-β（TGF-β） were significantly increased（P<0.05）, the content of interleukin-1β（IL-1β）,interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） were significantly decreased（P<0.05）, and serum endotoxin（ET-1） content and diamine oxidase（DAO） activity were significantly decreased（P<0.05）. The study indicates that adding compound plant extracts to diet can improve the antioxidant performance and immune function of weaned piglets, and improve the intestinal mucosal barrier function of piglets. [ABSTRACT FROM AUTHOR]

目的探究超脉冲 CO2 点阵激光联合低浓度 5-氟尿嘧啶对烧伤后增生性瘢痕患者的影响。方法回顾性分析自 2019 年1 月至2021 年9 月，川北医学院附属医院整形美容烧伤外科收治118 例烧伤后增生性瘢痕患者，按治疗方法分为两 组，A 组采用低浓度 5-氟尿嘧啶单独治疗；B 组采用超脉冲 CO2 点阵激光联合低浓度 5-氟尿嘧啶治疗。治疗3 个月后比较两 组患者的临床总有效率、温哥华瘢痕量表（vancouver scar scale, VSS）、双向瘢痕评估量表（patient and observer scar assessment scale, POSAS）评分、视觉模拟评分法（visual analogue scale, VAS）、数字评分法（numeric rating scale, NRS）及不良反应发生情况。 结果两组患者均有治疗效果，B 组总有效率大于A 组（P<0.05）。治疗后患者瘢痕的厚度、颜色、柔软度、血管分布情况都有 明显的好转，且B 组的VSS 总评分明显低于A 组（P<0.05）。B 组POSAS、VAS、NRS 评分均低于A 组（P<0.05）。治疗过程中两组 患者不良反应发生情况比较，差异无统计学意义（P>0.05）。结论采用超脉冲 CO2 点阵激光联合低浓度 5-氟尿嘧啶治疗烧伤 后增生性瘢痕可以减轻患者疼痛、瘙痒症状，改善瘢痕状态，提高皮肤美观度，且该治疗方案安全性较高。 [ABSTRACT FROM AUTHOR]

A total of 40 Colletotrichum strains causing anthracnose on Polygonatum kingianum were isolated and identified by morphology and ITS sequence in 2019. They were collected from three producing regions in Yunnan Province. Their sensitivities to fluazinam, prochloraz and difenoconazole were tested using the mycelium growth rate method. The results showed that all the 40 strains isolated from P. kingianum diseased leaves might belong to genus of Colletotrichum, i.c. C. gloeosporioides, C. fructicola, C. boninense and C. acutatum. Among them, 29 strains belong to C. fructicola, accounting for 72.5% of the total isolated strains. Fungicide sensitivity tests indicated that fluazinam, prochloraz and difenoconazole showed strong antifungal activities against the growth of all the tested strains. The EC50 values of fluazinam, prochloraz and difenoconazole to all tested strains were in the range of 0.028-0.53 mg/L, 0.018-2.69 mg/L and 0.158-2.57 mg/L, respectively. The sensitivities of all strains to fluazinam were higher than those to prochloraz and difenoconazole. No resistant strains to fluazinam, prochloraz and difenoconazole were found in those three producing regions of Yunnan Province. [ABSTRACT FROM AUTHOR]

A total of 40 Colletotrichum strains causing anthracnose on Polygonatum kingianum were isolated and identified by morphology and ITS sequence in 2019. They were collected from three producing regions in Yunnan Province. Their sensitivities to fluazinam, prochloraz and difenoconazole were tested using the mycelium growth rate method. The results showed that all the 40 strains isolated from P. kingianum diseased leaves might belong to genus of Colletotrichum, i.c. C. gloeosporioides, C. fructicola, C. boninense and C. acutatum. Among them, 29 strains belong to C. fructicola, accounting for 72.5% of the total isolated strains. Fungicide sensitivity tests indicated that fluazinam, prochloraz and difenoconazole showed strong antifungal activities against the growth of all the tested strains. The EC50 values of fluazinam, prochloraz and difenoconazole to all tested strains were in the range of 0.028-0.53 mg/L, 0.018-2.69 mg/L and 0.158-2.57 mg/L, respectively. The sensitivities of all strains to fluazinam were higher than those to prochloraz and difenoconazole. No resistant strains to fluazinam, prochloraz and difenoconazole were found in those three producing regions of Yunnan Province. [ABSTRACT FROM AUTHOR]

In order to investigate the genetic diversity and variation of different body colors of common carp (Cyprinus carpio), a total of 150 individuals from six different colored koi carp populations (Platinum Ogon, Higoi, Kohaku, Tajsho-sanke, Shiro Utsuri and Hi Utsuri), two colored FFRC No.2 strain common carp (grey and red) populations, and two hybrid carp populations (grey FFRC No.2 strain common carp♀ × Kohaku koi♂,Kohaku koi♀ × grey FFRC No.2 strain common carp♂) were analyzed based on mtDNA D-loop sequences. The results showed that the contents of A, T, G and C in the 932 bp D-loop sequence were 33.2%, 32.8%, 14.2% and 19.8%, respectively. In total, 34 variant sites were detected in all individuals, showing 18 haplotypes. Among them, there were two dominant haplotypes in Kohaku and Hi Utsuri, respectively. There were two unique haplotypes in white koi carp and grey FFRC No.2 strain common carp, and grey and red FFRC No.2 strain common carp shared one dominant haplotype. The genetic distances across the ten populations ranged from 0.001 to 0.018, with FST values ranging from 0.011 to 0.933. The analysis of molecular variance (AMOVA) revealed that there was 67.17% variation among varieties and 32.83% variation within varieties, indicating highly substantial genetic difference. It demonstrated that the Hi Utsuri, white koi carp, and Tajsho-sanke had high genetic diversity, while the Shiro Utsuri, FFRC No.2 strain common carp (grey and red), and hybrid carp had high genetic purity. The study enriched the genetic data of the various body color carp germplasm resources and provided the guidance for germplasm resource utilization and genetic selection in the future. [ABSTRACT FROM AUTHOR]

Objective Dyslipidemia has shown to be associated with cardiovascular, metabolic and renal diseases. This study aimed to investigate the association between residual cholesterol and the risk of subclinical renal damage (SRD). Methods A total of 2 342 participants were recruited from the previously established Hanzhong Adolescent Hypertension Study cohort. According to estimated glomerular filtration rate(eGFR) and urinary albumin-to-creatine ratio(uACR), the subjects were divided into SRD group and non-SRD group. The associations of residual cholesterol with eGFR, uACR, and the risk of SRD were analyzed by multiple linear and Logistic regression analyses. Results Residual cholesterol was positively correlated with uACR(r=0.081, P<0.001) but negatively correlated with eGFR (r=-0.091, P<0.001). Multiple linear regression analysis revealed that residual cholesterol was an influencing factor of uACR (β=0.075, P<0.001) and eGFR (β=-0.027, P<0.001) after adjustment for gender, age, smoke, alcohol, exercise, BMI, hypertension, diabetes and serum uric acid. In addition, Logistic regression analysis revealed that residual cholesterol was significantly associated with the risk of SRD independently of potential confounders [OR(95% CI)=1.387 (1.113-1.728), P<0.001]. Further subgroup analysis showed that residual cholesterol was significantly associated with the risk of SRD in women but not in men. Conclusion Residual cholesterol is a contributing factor in the risk of subclinical renal damage with gender-specific association. [ABSTRACT FROM AUTHOR]

Objective Based on our previously established salt-sensitive hypertension cohort, we aimed to examine the association of genetic variants in uromodulin with blood pressure(BP) responses to dietary interventions of sodium and potassium intake. Methods In 2004, 514 subjects from 124 families in Mei County, Shaanxi Province, were recruited to establish the salt-sensitive hypertension study cohort. Among them, 333 non-parent subjects were selected and sequentially maintained on a normal-diet for 3 days, low-salt diet for 7 days, then a high-salt diet for 7 days and a high-salt diet with potassium supplementation for another 7 days. Thirteen single nucleotide polymorphisms(SNPs) in the uromodulin gene were genotyped on the MassARRAY platform. Results BP levels decreased from the baseline to low-salt diet, increased from low-salt to high-salt diet, and decreased again from the high-salt diet to the high-salt plus potassium supplementation intervention. SNPs rs77875418 and rs4997081 of the uromodulin gene were significantly associated with diastolic BP(DBP) and mean arterial pressure(MAP) responses to high-salt diet. In addition, SNPs rs77875418, rs79245268, rs4293393, rs6497476, rs4997081, rs13333226, and rs12917707 were significantly associated with systolic BP(SBP), DBP, and MAP responses to high-salt diet with potassium supplementation. Conclusion Genetic variants in uromodulin gene are significantly associated with BP responses to sodium and potassium supplementation, suggesting that uromodulin may be mechanistically involved in BP sodium-sensitivity and potassium-sensitivity. [ABSTRACT FROM AUTHOR]

Objective 4-like protein with down-regulated expression and development in neural precursor cells (NEDD4L) plays an important role in blood pressure (BP) regulation and sodium homeostasis by regulating epithelial sodium channel protein. In this study, we aimed to explore the relationship of NEDD4L gene polymorphisms with BP responses to sodium and potassium intake. Methods In 2004, 514 subjects from 124 families in Meixian County, Shaanxi Province, were recruited to establish a salt-sensitive hypertension study cohort. All the subjects received a 3-day baseline survey, a 7-day low-salt diet, a 7-day high-salt diet, and finally a 7-day high-salt and potassium supplementation. Their BP was measured and peripheral blood samples were collected at different intervention periods. The 14 gene polymorphisms of NEDD4L gene were genotyped and analyzed by MassARRAY platform. Results BP decreased on a low-salt diet, and significantly increased on a high-salt diet, and decreased again after potassium supplementation. NEDD4L SNPs rs74408486 were significantly associated with systolic BP, diastolic BP and mean arterial pressure responses to the low-salt diet. SNPs rs292449 and rs2288775 were significantly associated with pulse pressure response to the high-salt diet. In addition, SNPs rs563283 and rs292449 were significantly associated with diastolic BP, mean arterial pressure, and pulse pressure responses to high-salt and potassium supplementation diet. Conclusion NEDD4L gene polymorphisms were significantly associated with BP responses to sodium and potassium intake, suggesting that NEDD4L gene may be involved in the development of salt sensitivity and potassium sensitivity. [ABSTRACT FROM AUTHOR]

Objective M3 muscarinic acetylcholine receptor(M3 receptor), encoded by CHRM3 gene, is widely distributed in the cardiovascular system and plays an important role in cardiac regulation. The aim of this study was to assess the association of genetic variants in M3 receptor with blood pressure(BP) responses to controlleddietary sodium and potassium interventions. Methods A total of 333 subjects from 124 families were recruited from the rural areas of northern China. After a three-day baseline observation, they were sequentially on a seven-day low-salt diet, a seven-day high-salt diet, and a seven-day high-salt diet plus potassium supplementation. Thirteen CHRM3 single nucleotide polymorphisms(SNPs) were selected for analysis. Results SNP rs10802811 of the CHRM3 was significantly associated with diastolic BP(DBP) and mean arterial pressure(MAP) responses to both low-salt and high-salt diets while SNPs rs6429147, rs373288072, rs114677844 and rs663148 showed significant associations with systolic BP(SBP) and MAP responses to high-salt diet. In addition, SNP rs6692904 was significantly associated with SBP, DBP and MAP responses to high-salt diet with potassium supplementation. Conclusion Genetic variants in M3 receptor are significantly associated with BP responses to sodium and potassium intervention, suggesting that M3 receptor may be mechanistically involved in BP salt and potassium sensitivity. [ABSTRACT FROM AUTHOR]

BACKGROUND: For elderly patients with hip fractures, improving the ability to screen for deep vein thrombosis of the lower extremities and implementing individualized prevention programs are important measures to improve the prognosis of patients and reduce the burden on patients' families and socioeconomics. OBJECTIVE: To investigate the risk factors of deep venous thrombosis in elderly patients with hip fracture after operation, and to establish a risk model to predict the occurrence of deep venous thrombosis. METHODS: Clinical data of 325 hospitalized patients with hip fracture over 70 years old from January 2017 to December 2019 were retrospectively analyzed, and they were divided into deep venous thrombosis group (n=50) and non-deep venous thrombosis group (n=275) according to the results of deep vein color Doppler ultrasound examination of lower extremities. The univariate and multivariate Logistic regression analyses were used to identify the independent risk factors for postoperative deep venous thrombosis in elderly patients with hip fracture. Receiver operating characteristic curve was used to analyze the diagnostic efficacy of independent risk factors and combined models for deep venous thrombosis. Furthermore, R Studio software was used to construct a column chart to predict the risk of postoperative deep venous thrombosis of lower limbs in elderly patients with hip fracture, and calibration curves were made to verify the accuracy of the model. RESULTS AND CONCLUSION: (1) The univariate analysis results showed that age, fracture type, the time between injury to operation, anesthesia, operation time, preoperative basic diseases, potassium, cholesterol, creatinine, and total protein were significantly different between the two groups (P < 0.05). (2) Multivariate Logistic regression analysis showed that fracture type of intertrochanteric fracture of femur, time from injury to operation, presence of diabetes before operation, and cerebrovascular disease were independent risk factors for postoperative deep venous thrombosis in elderly patients with hip fracture (P < 0.05). (3) Receiver operating characteristic curve of each independent risk factor and combined model exhibited that the time from injury to operation, the presence of diabetes, cerebrovascular disease, and the combined model had predictive ability, but the type of hip fracture did not have predictive ability, and the combined model had better predictive ability than the single independent risk factor. (4) The column chart has good diagnostic efficiency and accuracy. [ABSTRACT FROM AUTHOR]

Objective To observe the effects of miR-128 and ZEB1 on the migration, invasion and epithelial mesen‐ chymal transition( EMT) of endometrial carcinoma( EC) cells and to verify the targeted relationship between them. Meth⁃ ods The qRT-PCR and Western blotting were used to detect the relative expression levels of miR-128 and ZEB1 in three EC cell lines( HEC-1A, Ishikawa, and KLE) and one endometrial epithelial cell line hEEC, respectively. Ishikawa cell line with the lowest expression level of miR-128 was taken as the follow-up study object. Ishikawa cells were divided into the negative control group and miR-128 overexpression group, and were transfected with miRNA-NC and miR-128 mim‐ ics, respectively. After 48 h, the cells were collected for follow-up experiments. Cell Scratch test and Transwell test were used to detect the migration and invasion abilities of cells in each group after transfection. Western blotting was used to de‐ tect ZEB1, E-cadherin, Fibronectin and Vimentin protein of cells in each group after transfection. Double luciferase re‐ porter gene experiment was used to verify the targeted regulatory relationship between miR-128 and ZEB1. Results Com‐ pared with hEEC, the expression of miR-128 in EC cell lines HEC-1A, Ishikawa and KLE decreased( all P<0. 05), and the expression of ZEB1 protein increased( P<0. 05) . After transfection, the expression of miR-128 increased significantly in Ishikawa cells( P<0. 05), the number of transmembrane cells and scratch healing rate decreased( all P<0. 05), the rel‐ ative expression levels of ZEB1, Fibronectin and Vimentin decreased( all P<0. 05), and the expression of E-cadherin protein increased( P<0. 05). The results of double luciferase reporter gene experiment showed that miR-128 could target and regulate ZEB1 gene( P<0. 05). Conclusions MiR-128 can inhibit the migration, invasion and EMT of Ishikawa cells. ZEB1 can promote the migration, invasion and EMT of Ishikawa cells. MiR-128 and ZEB1 have a targeted regulatory rela‐ tionship. [ABSTRACT FROM AUTHOR]

The acidizing fracturing flowback fluid with strong corrosion, high viscosity, high oil content and high suspended matter content was treated with "viscosity reduction, neutralization, de-stabilization and filtration". The results show that the optimal viscosity reduction conditions were as follows: pH was 4, the dosing quantity of H2O2 was 2 000 mg/L, the dosing quantity of Fe2+ was 500 mg/L, reaction time was 60 min and the optimal instability conditions were as follows: pH was 8, the dosing quantity of PAFC was 120 mg/L, the dosing quantity of PAM was 1. 2 mg/L. Under the optimized conditions and after comprehensive treatment, the oil content and SS content of the acidizing fracturing flowback fluid were 4. 4 mg/L and 1. 3 mg/L, the average corrosion rate was 0. 035 mm/a, the viscosity was 1. 4 mPa • s. The treated acidizing fracturing flowback fluid was mixed with formation water in any proportion, and the compatibility was good. All indicators meet the standard of re-injection. [ABSTRACT FROM AUTHOR]

A new inorganic-organic silicon-aluminum composite flocculant was synthesized by silane coupling agent γ-aminopropyltriethoxysilane and aluminum chloride under alkaline condition.The results show that the best synthetic conditions are as follows: the total aluminum concentration (AlT) is 0.2 mol/L, the molar ratio of Si/Al is 0.6 and the alkalization degree B value is 2.0.The pH value of the new inorganic-organic silicon-aluminum composite flocculant has positive correlation with B value and Si/Al molar ratio, and Zeta potential has negative correlation with B value and Si/Al molar ratio. The organic and inorganic components in the flocculant are bonded together by covalent bond, and the flocculant has a dense space network structure and excellent performance of adsorption and bridging. [ABSTRACT FROM AUTHOR]

Objective · To explore the relationship among perceived stress, personality traits and panic disorder (PD) symptoms, and to test the mediating effect of perceived stress on PD symptoms in different personality traits. Methods · A total of 55 PD patients were selected according to Diagnostic and Statistical Manual of Mental Disorders (Fifth Edition) (DSM-5). The NEO Five-Factor Inventory (NEO-FFI) was used to measure the personality traits. The Panic Disorder Severity Scale (PDSS) was used to measure the PD symptoms severity. The Perceived Stress Scale (PSS) was used to measure the level of the perceived stress. Results · Both the neuroticism, agreeableness subscale scores of NEO-FFI and scores of PSS were significantly correlated with the total scores of PDSS, and the correlation coefficient were r0.379 (P0.004), r-0.420 (P0.001) and r0.330 (P0.014), respectively. The mediating effect analysis showed that perceived stress completely mediated the effect of agreeableness on PD symptoms, and the mediating effect was 32.43%. Conclusion · In personality traits, neuroticism may be positively correlated with PD symptoms, while agreeableness may be negatively correlated with PD symptoms. The perceived stress may play a completely mediating role between agreeableness and PD symptoms. [ABSTRACT FROM AUTHOR]

In this paper, the effect of streptozotocin (STZ) on gonadal function in diabetic mice was studied. Seventy male Kunming mice were randomly divided into control group (10 rats) and model group (60 rats). Model group was intraperitoneally injected with STZ (75 mg./kg) for 2 days, 1 time/d,and the control group. The citrate buffer was injected intraperitoneally. Fasting blood glucose (FBG) was measured 72 hours after the last injection, and the mice in the model group with blood glucose≥11. 1 mmol/L were taken for follow-up test. Fasting blood glucose was measured at 7 d, 14 d,21 d and 28 d,and the mating experiment was carried out. The number of straddles and the number of matings were sacrificed after the 28 d mating experiment, and serum testosterone levels were determined. The results showed that compared with the control group,the blood glucose of the model group was sig-nificantly increased at 7 d,14 d.21 d and 28 d after STZ injection (P<0.05); and the number of straddles in the mating test at 7 d, 14 d,21 d and 28 d and The number of matings was significantly reduced (P<0.05,P<70.01), and serum testosterone levels were significantly decreased in 28 d mice (P<0. 01). Therefore,STZ low-dose multiple intraperitoneal injection can successfully induce hypogonadism in diabetic mice. [ABSTRACT FROM AUTHOR]

目的分析住院医师规范化培训(住址)医师对新型冠状病毒肺炎(COVID 19)的认迦、个人防护措施及心理支 化,为指导医院培训管理返岗位培医师提供科学依据。方法选取河南省人民医院住培医师作为研究对象,采用微信群进行问卷 调查,截至2020年3月6日17:30 收集问卷 1 242 份,有效问卷 1 161 份,有效率为93.5%。结果1 161 名住址医师中存在心理 应漱者占20.8%(242/1 161),对 COVID 19 传播途径、流行病史知晓率达 84.6%~90.4%,影像学检查特点知晓率达10.3%~ 85.1%,诊断及出院标准知晓率达73.0%~83.4%,国务人员个人防护知晓率达54.5%~91.1%.COVID 19 高认知住址医师个 人防护措施得分明显高于低认知者,差异有统计学意义(P<0.05)。结论住培医师对 COVID 19 相关知识具有较好的认知,对 医务人员的个人防护措施认识不足,存在心理应激现象,应加强住培医师临床工作中个人防护措施的培训,拓展培训资源,增加 专业知识的学习机会,针对性地进行心理疏导. [ABSTRACT FROM AUTHOR]

Field experiments were conducted to evaluate cotton aphids (Aphis gossypii Glover) control efficacy of insecticides spray drone, MG-1S unmanned aerial vehicle (UAV).The results showed that control efficacy of 22% sulfoxaflor SC at 225 mL/hm2 was better than 70% acetamiprid WG at 60 g/hm2 and 70% imidacloprid WG at 30 g/hm2. The adjuvant Gaogongxiao at 15 mL/hm2 was recommended for optimum cotton aphid control in combination with 70% acetamiprid WG at 60 g/hm2 and 70% imidacloprid WG at 30 g/hm2. Flight control synergist at 150 mL/hm2 could be co-applied with 22% sulfoxaflor SC at 150 mL/hm2, following by the adjuvants of mineral oil, silicone and polymer additives.There was no significant difference between UAV spraying and tractor machine and manual tow spraying on the control efficacy of cotton aphid at the 3rd and 5th days after the application. However, UAV field application could decrease the spray volume of pesticides by 1/3, increase work efficiency by 3 times, and reduce the labor cost by 60 yuan/hm2. UAV could meet the needs of modern agriculture and achieve efficiency increasing , pesticide saving and cost reduction [ABSTRACT FROM AUTHOR]

Objective To investigate the long-term efficacy of PEG-IFN alpha-2a (PEG-IFNα-2a)plus ribavirin (RBV) in treatment of chronic hepatitis C (CHC) patients with IL28B single nucleotide polymorphisms (SNPs) rs12979860 C/C type in different HCV genotypes. Methods A prospective study was conducted on 38 CHC patients from our hospital’s Infection Department from March 2011 to September 2015. The patients were treated with PEG-IFNα-2a/RBV for 48 weeks. A 42-month follow-up of patients was performed after withdrawal of treatment. The main paramenters to value the efficacy were liver function, blood lipids, and sustained virological response (SVR). Results In the CHC patients with IL28B SNP rs12979860 C/C type, the rate of SVR in patients with antiviral therapy had no significant difference between groups 1b and 2a (73.33% and 95.65%, respectively, P>0.05). After anti-HCV therapy, liver function indices such as ALT, AST, TBIL, TC, TG and HDL all significantly improved in the two groups (all P<0.05). However, there was no difference in biochemical indices (ALT, GGT, bilirubin, blood lipids) between the two groups (all P>0.05). Conclusion In CHC patients with IL28B SNP rs12979860 C/C type, the long-term efficacy of PEG-IFNα-2a/RBV is good. IFN-based antiviral therapy has a higher SVR rate, and liver function and lipid metabolism can be significantly improved. [ABSTRACT FROM AUTHOR]

A total of 27 wall specimens were tested under axial compression to investigate the compressive performance of stone masonry walls. The main test parameters included the strength of mortar, height-to-thickness ratio, and construction method (mortar-laid or dry-laid). The failure mode and load-deformation relationship of the specimens were studied. The influence of different parameters on the axial compressive capacity of stone masonry walls was investigated. During testing, crush of mortar and gaskets inside the bed joints as well as split of stone blocks were observed for all specimens, and the failure exhibited brittle features. The results show that when the height-to-thickness ratio is no more than 5.75, more gaskets inside the joints can effectively increase the load carrying capacity of the dry-laid specimens. With all other parameters set as the same, the mortar-laid specimens have higher stiffness and compressive strength than the dry-laid specimens. Finally, a formula, which takes into account the influence of height-to-thickness ratio, is proposed to predict the load carrying capacity of the dry-laid stone masonry walls, and the calculated results agree well with the test results. [ABSTRACT FROM AUTHOR]

Objective: To explore the influence factors of dynamic electrocardiogram (DCG) change after intensity modulated rediation therapy (IMRT) for patients with lung cancer. Methods: 161 lung cancer patients underwent IMRT in our hospital from February 2013 to February 2015 were selected. All patients underwent 24 h DCG and the results and the influence factors were analyzed. Results: The occurrence rate of sinus arrhythmia before and after treatment had no statistically significance (P>0.05). The occurrence rate of occasional atrial (ventricular) arrhythmias, frequent atrial (ventricular) arrhythmias, conduction block and changes of ST-T segment after treatment were all higher than before treatment, and the occurrence rate 6 months after treatment were obviously lower than after-treatment with statistically significance (P<0.05). The gender and V20-V50 of heart had influence on the changes of DCG. And the gender and V40 were the independent risk factors of abnormal DCG. Conclusions: The occurrence rate of occasional atrial (ventricular) arrhythmias, frequent atrial (ventricular) arrhythmias, conduction block and changes of ST-T segment is getting higher after IMRT for patients with lung cancer, and the gender and V40 are the independent risk factors of abnormal DCG. [ABSTRACT FROM AUTHOR]

Objective To investigate the therapeutic effect and mechanism of Caveolin-1 (Cav1) mutant to F92ACav1 modified rat bone marrow mesenchymal stem cells (rBMSCs/F92A-Cav1) on rats with pulmonary hypertension (PAH). Methods PAH was induced by intraperitoneal injection of 1% monocrotaline (MCT, 60 mg/kg) in adult male Wistar rats. The PAH rats were randomly divided into four groups( 10 rats/group) after 2 weeks of MCT injection:PAH group (only MCT), Cav1 group( transduced with LV-Cav1 modified r BMSCs), F92A-Cav1 group (transduced with LV-F92A-Cav1 modified r BMSCs),and the control group. Gene modified r BMSCs (1 × 106/ml) were transplanted into PAH rats in each group by tail vein injection. After 3 weeks of transplantation,the percentage of media wall thickness (MT%) and right ventricular hypertrophy index( RVHI) were evaluated by Image-Pro Plus 6 software,serum NO concentration was checked by Griess method, and the expression of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) was detected by Western blotting. Results MT%, RVHI, PI3K and AKT was all increased but NO was decreased in the PAH group as compared with that of the control group (P < 0. 05 or P < 0. 01). Compared with the PAH group, MT%, RVHI, PI3K and AKT was decreased but NO was increased in the Cav1 and F92A-Cav1 groups, especially in the F92A-Cav1 group (P < 0. 01). Conclusion F92A-Cav1 can abrogate the inhibitory effect of wild-type Cav1 on e NOS and promote the release of NO, then inhibit the activation of PI3K/Akt signaling pathway and thus exert its therapeutic effect on PAH rats. [ABSTRACT FROM AUTHOR]

Rainwater has become a big problem of the city, because rainwater led to urban waterlogging, affect urban transport. Urban waterlogging is also a kind of hidden danger for urban residents. At the other side, government spends a lot of money to meet municipal water demand, for example the South-to-North Water Transfer Project. Many cities land subsidence caused by groundwater exploitation. Urban agriculture which used rainwater as irrigation water is an effective way for urban rainwater as resources. The safety of urban agricultural products irrigated by rainwater is attention focus, because urban rainwater is polluted by automobile exhaust, asphalt, cement and so on. This paper study the food safety and nutrient quality of lettuce irrigated by urban rainwater aims to explore the feasibility of urban rainwater as urban agricultural irrigation water. A hydroponics experiment was carried out from October 2014 to January 2015 in Hainan University. Four kinds of treatments were designed using hydroponic lettuce design with four replicates, respectively: hydroponic lettuce using tap water (CK), hydroponic lettuce using rainwater (T1), hydroponic lettuce using rooftop rainwater (T2), and hydroponic lettuce using road rainwater (T3). 3 times rainwater from rainwater, rooftop rainwater of office and road rainwater were collected from October in 2014 to January in 2015. The rainwater was mixed respectively, allowed to stand for precipitating impurities, was used as nutrient solution water. Lettuce was cultured in tap plastic pipe. Plant height, leaf number, physiological indexes (chlorophyll content, net photosynthetic rate, root activity), nutritional indexes (soluble protein content, soluble sugar content, vitamin C content, nitrate content), and element (Ca, Fe, Mg, Zn, As, Cd, Pb) content in the lettuce mature stage under different rainwater nutrient solution and tap water nutrient solution were measured. The results show that heavy metal content of rainwater is lower than national agricultural irrigation water standards, urban rainwater is safety to be used as urban agriculture irrigation water. As content of lettuce was respectively 5.83 (CK), 4.10 (T1), 4.53 (T2), 4.60 μg/kg (T3), Cd content of lettuce was respectively 0.76 (CK), 0.78 (T1), 2.59(T2), 1.37 μg/kg (T3), Pb content of lettuce was respectively 102.37 (CK), 118.63 (T1), 151.53 (T2), 123.37 μg/kg (T3). They were less than the national limit values (As content 500 μg/kg, Cd content 200 μg/kg, Pb content 300 μg/kg), the lettuce meet the food safety requirements. Proteins content, sugars content and vitamin C content of lettuce cultivated in rainwater was not lower than that in rainwater; Ca, Mg content of lettuce cultivated in rainwater is higher than that in tap water, Fe content of lettuce cultivated in rainwater is less than that in tap water. The quantity of lettuce cultivated in rainwater is better than that in tap water. Chlorophyll content and net photosynthetic rate of lettuce cultivated in rainwater is lower than that cultivated in tap water. Root activity of lettuce cultivated in rainwater was increased. Root-shoot ratio of lettuce cultivated in rainwater was respectively 0.19(T1), 0.11(T2), 0.12 (T3), they were bigger than that cultivated in tap water(0.08). Lettuce cultivated in rainwater has worse growth power and lower biomass yield than lettuce cultivated in tap water. Lettuce biomass yield cultivated in road rainwater is lowest in three kind of lettuce cultivated in rainwater. In short, the urban rainwater and the agricultural produces cultivated by urban rainwater is safety, and the quality of agricultural produce cultivated in urban rainwater is better. At the same time, crop irrigated by urban rainwater grows weak and has lower yield. Experiments on lettuce demonstrate that urban rainwater is used as urban agriculture irrigation water is feasible. [ABSTRACT FROM AUTHOR]

Four stone masonry wall specimens strengthened with steel-meshed modified cement mortar were tested under cyclic horizontal loading. Parameters investigated included the strength of modified cement mortar, strength of reinforcing steel bars, and strengthening modes ( single-faced or double-faced). The failure mode, bearing capacity, hysteretic loops, skeleton curve, and energy-dissipation capacity of specimens were investigated. It is found that the shear strength and energy-dissipation capacity of the strengthened walls are increased significantly compared to the unstrengthened walls. Adopting double-faced strengthening method and increasing mortar strength can further improve the shear strength, and the former one is much more effective. An empirical model to calculate the bearing capacity of strengthened walls is proposed and the predicted values agree well with the test ones. [ABSTRACT FROM AUTHOR]

组织学是一门研究显微镜下健康人体结构及其功能的医学入门课，是医学生学习临床课程的基础，但因 该课程具有知识点抽象、繁杂的特点，学生往往反映难懂、难记。为了激发学生自主学习的兴趣，提高其学习效率，促进组 织学教学的信息化发展，尝试将微课引入组织学的传统教学，利用微课的优势，生动、直观地展示显微镜下的结构，有效 弥补了传统教学的不足，丰富了教师的教学模式，并满足现代学生个性化的学习需求。该文以组织学的滤过屏障内容为 例，探索组织学微课的设计制作和应用，为将微课和传统教学在组织学中有效融合提供借鉴。 [ABSTRACT FROM AUTHOR]

Objective: To study the risk factors in elderly upper gastrointestinal bleeding and the correlation with HP infection Methods: 300 cases of peptic ulcer patients diagnosed by barium meal, and gastroscopy in our hospital were selected as objects, which included 125 cases that combined with hemorrhage, and 175 cases without hemorrhage. And 310 cases exclusive of peptic ulcer in the same period by endoscopy were considered as control group. HP infection and use of non steroidal anti-inflammatory drugs(NSAIDs) were detected and carried on statistics analysis. Results: ① The OR value of incidence of peptic ulcer in HP combined with NSAIDs group was 20.31, obviously higher than that of HP group 6.97 and NSAIDs group 6.73, and more than the sum of the two, the difference was statistically significant(P0.05); ② for the relationship between peptic ulcer combined with hemorrhage and NSAIDs drug dose and time, the results showed the OR value in B group was 3.79, and 7.88 in C group, 10.41 in D group, group B group Cgroup D, the difference was statistically significant(P0.05); ③ for the occurrence of peptic ulcer combined with hemorrhage, the OR value in HP combined with NSAIDs group was 13.31, significantly higher than that of group HP 1.12 and group NSAIDs 1.54, and more than the sum of the two, the difference was statistically significant(P0.05). Conclusions: The occurrence of elderly upper gastrointestinal hemorrhage is mainly associated with peptic ulcer, and the infection of HP and taking NSAIDs drugs, have synergistic effect in the course of disease. [ABSTRACT FROM AUTHOR]