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Objective To investigate the influencing factors of accuracy of 6-minute walking test (6MWT) for patients with coronary heart disease, and to summarize strategies to improve the quality of 6MWT. Methods The oxygen uptake and heart rate at anaerobic threshold in the Cardiopulmonary Exercise Test (CPET) were set as the standards of testing, and the peak oxygen uptake and maximal heart rate of 6MWT among different groups were analyzed by chi-square test. The improvement plan was made based on the influencing factors analysis. Results There was a significant difference between oxygen uptake at anaerobic threshold in CPET and peak oxygen uptake in 6MWT(P＜0. 01)among all participants, and there was a significant difference between oxygen uptake at anaerobic threshold in CPET and peak oxygen uptake in 6MWT in participants with symptoms and aged between 50 and 71 years old(P＜0. 01). There was a significant difference between heart rate at anaerobic threshold in CPET and maximal heart rate in 6MWT(P＜0. 01)among all participants, and There was a significant difference between heart rate at anaerobic threshold in CPET and maximal heart rate in 6MWT in participants with symptoms and aged between 50 and 71 years old(P＜0. 01). Conclusion Taking CEPT as an standard, factors influencing the outcomes of 6MWT should be taken into consideration, and improvement measures should be carried out to improve the test accuracy. It is suggested that quality of 6MWT should be strengthened from following aspects: patient health education, kinesiophobia, financial burden, time of conducting 6MWT after PCI and role of cardiac rehabilitation specialist nurses. (目的 研究影响冠心病患者6分钟步行试验(6MWT)评估准确性的原因并分析总结方法, 提高6MWT完成质量。方法 以心肺功能运动试验(CPET)的无氧阈摄氧量与无氧阈心率作为达标标准, 使用卡方检验分析不同分组的6MWT峰值摄氧量与最大心率数据, 探讨测试结果的原因, 并制定改善方案。结果 全体受试者CPET中的无氧阈与6MWT中的峰值摄氧量比较, 差异有统计学意义(P＜0. 01); 不同组别间CPET中的无氧阈与6MWT中的峰值摄氧量比较, 有症状组、老年组差异有统计学意义(P＜0. 01)。全体受试者CPET无氧阈心率与6MWT最大心率比较, 差异有统计学意义(P＜0. 01), 不同组别间CPET中的无氧阈与6MWT中的峰值摄氧量比较, 有症状组、老年组差异有统计学意义(P＜0. 01)。结论 以CEPT作为标准分析影响6MWT结果的原因并提出改善方法, 从而提高6MWT的准确性; 根据结果分析对患者健康教育、运动恐惧、经济负担、PCI术后6MWT评估时间及心脏康复专科护士角色功能几个方面提出改善方法并实施。)

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Copyright of Evaluation & Analysis of Drug-Use in Hospitals of China / Zhongguo Yiyuan Yongyao Pingjia yu Fenxi is the property of Evaluation & Analysis of Drug-Use in Hospitals of China Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The eastern Wehai area, bordered by the Mishan Fault, is an important polymetallic mineralization zone in the Jiaodong region. It hosts a series of copper molybdenum, lead zinc, and gold silver polymetallic mineral deposits related to the late Yanshanian Mesozoic intermediate-acidic intrusive magmatic activity. This study investigates the geochemical characteristics, metallogenic age, and ore genesis of the Kuangbei skarn-type copper deposit, a typical deposit in the area. Testing results of rare earth elements (REEs) and micro-elements suggest that the intrusive activities of Weideshan Period magmatic rocks provided both the metallogenic materials and the hydrothermal conditions essential for the deposit formation. Research on stable isotopes of S and Pb indicates a multi-source origin of the ore-bearing hydrothermal fluids. Zircon U-Pb dating reveals that the formation age of Kuangbei Copper Deposit, approximately 116. 6 Ma, is close to the diagenetic age of Weideshan Period magmatic rocks. This deposit is a Mesozoic contact metasomatic (skarn) type copper deposit. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Diabetes Mellitus is the property of Chinese Journal of Diabetes Mellitus and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

在人工养殖中，乌苏里蝮健康情况值得关注。为了探究病情，并为以后有效地预防和治疗提供资料，本研究室对养殖种群进行了筛查。针对其中颈部有异常肿大的个体进行了血液学检测、组织学观察和免疫组化鉴定。观察结果显示：患病蛇脂肪体系数显著大于正常蛇；白细胞和血栓细胞总数显著低于正常蛇，糖链抗原24-2 呈阳性。肿块的组织学切片及免疫组化染色结果中可见典型的上皮细胞肿瘤的特征结构——癌穴，常规用于鉴定肿瘤的标记物S100、VIM、EMA和GFAP均呈阳性，故确定该肿大物为间叶型软骨肉瘤，但诱发原因还需要进一步研讨。建议在人工繁育乌苏里蝮的过程中，需要精选种蛇，并对养殖种群进行定期的筛选，提高其健康程度。

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OBJECTIVE: To observe the neuroregulatory effects of Chaihu Shugan powder and Banxia Houpo decoction combined with Wuxing acupuncture on anxiety and depression after stroke and its effects on immune inflammatory response, cognitive function impairment, hypothalamic-pituitary-gonadal axis (HPG) and hypothalamicpituitary-thyroid axis (HPT). METHODS: A total of 102 patients with post-stroke anxiety and depression admitted into the hospital from Mar. 2021 to May 2023 were extracted to be divided into 51 cases in the Wuxing acupuncture group (2 cases were shed and 49 cases were eventually included), and 51 cases in the prescription combination group (2 cases were shed and 49 cases were eventually included) according to the random number table method. Both groups were given conventional Western medicine basic treatment, the Wuxing acupuncture group received Wuxing acupuncture treatment based on the conventional Western medicine basic treatment, and the prescription combination group was treated with Chaihu Shugan powder and Banxia Houpo decoction on the basis of the Wuxing acupuncture group. Clinical efficacy of two groups was compared. Traditional Chinese medicine syndrome score, Mini-Mental State Examination ( MMSE) score, Generalized Anxiety Disorder Scale ( GAD-7) score, Hamilton Depression Scale (HAMD) score and Quality of Life Enjoyment and Satisfaction Questionnaire, Short Form (Q-LES-Q-SF) score of two groups before and after treatment were compared. Serum levels of nerve growth factor ( NGF), interleukin-1β (IL-1β), glial fibrillary acidic protein (GFAP), interleukin-17A (IL-17a), thyroid stimulating hormone (TSH), 5-hydroxytryptamine (5-HT) and estradiol 2 (E2) were measured before and after treatment. RESULTS: The total effective rate of the prescription combination group was 97. 96% (48/49), higher than 81. 63% (40/49) of the Wuxing acupuncture group, the difference was statistically significant (P < 0. 05). After treatment, the scores of anxiety and depression, epigastric fullness, chest and hypochondriac distension, frequent sighing in the prescription combination group were lower than those in the Wuxing acupuncture group, the scores of HAMD and GAD-7 were lower than those in Wuxing acupuncture group, and the scores of MMSE and Q-LES-Q-SF were higher than those in Wuxing acupuncture group, with statistically significant differences (P<0. 05). After treatment, levels of of IL-1β, IL-17a and GFAP in prescription combination group were lower than those in Wuxing acupuncture group, and levels of NGF in prescription combination group were significantly higher than those in Wuxing acupuncture group, levels of 5-HT, E2 and TSH in prescription combination group were higher than those in Wuxing acupuncture group, with statistically significant differences (P<0. 05). CONCLUSIONS: Chaihu Shugan powder and Banxia Houpo decoction combined with Wuxing acupuncture in the treatment of patients with post-stroke anxiety and depression can reduce the immune inflammatory response and neurological and cognitive function damage, regulate HPG and HPT, improve anxiety and depression, relieve the disease, improve quality of life and clinical efficacy. [ABSTRACT FROM AUTHOR]

The aim of this study was to develop heat-tolerant cellulase enzymes to improve the utilization rate of cellulosic feed and the economic benefits of livestock and poultry. The sequence information of an exonuclease CelS from Clostridium thermocellum was obtained from the protein information database UniProt, and the overexpression of CelS was achieved by signal peptide deletion and codon optimization, and the expression system of Escherichia, coli was used. Through the optimization of self-induced expression conditions, the proportion of soluble expression of CelS was effectively increased. The intracellular supernatant was separated and purified by nickel ion affinity chromatography and heat treatment to obtain soluble CelS, and the intracellular precipitate was washed, denatured and renatured to obtain inclusion body renatured CelS. The enzymatic characteristics showed that there was no significant difference in the enzyme activities of the two forms of CelS, the optimal reaction temperature was 70 贮, the optimal reaction pH was 5.5-6.0, and the activity of amorphous cellulose (PASC) was the highest, followed by crystalline cellulose (Avicel) and corn stover (CS). CelS and EG12B synergistically hydrolyzed CS with Thermotoga maritima, which increased the activity of hydrolase by 81.8% compared with hydrolase alone. The results showed that recombinant CelS exhibited superior heat tolerance and cellulose hydrolysis activity, which provided strong support for the further development of enzyme preparations, and was conducive to improving the utilization rate of cellulose feed. [ABSTRACT FROM AUTHOR]

Intellectual property security is the main battlefield of national security, and strengthening intellectual property security review is an important measure to protect national security and improve intellectual property security protection in the field of intellectual property. In the new era, the security review mechanism of intellectual property rights transfer and its implementation are facing double pressures worldwide, which need to be further adjusted and improved. This study analyzes the institutional games and challenges brought about by the changes in the international situation of intellectual property rights transfer, clarifies the requirements of the current overall national security concept and the strategy of self-reliance and self-reliance in science and technology for intellectual property rights transfer, sorts out many problems existing in the security review mechanism and specific implementation of intellectual property rights transfer, and puts forward the optimization ideas for improving the security review system and implementing the system. In theory, the institutional dilemma of the security review mechanism of intellectual property rights transfer abroad is clarified, which can provide research reference for the security review of intellectual property rights transfer in China in practice. [ABSTRACT FROM AUTHOR]

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在对人工养殖的棕黑锦蛇体质测量的研究中发现一例蛇肾脏出现形态异常后,应用大体解剖和组织学方法对其进行深入观察,并检测血液指标的变化。肾脏异常表现为2个肾脏形状和大小明显不同,其中左肾明显肥大,肾系数约为正常肾脏的2倍; 而右肾则明显萎缩,肾系数仅为正常肾脏的1/6。组织活检表明,左肾实质组织结构基本正常,而右肾实质变得密集,肾单位之间增生有丰富的纤维性结缔组织。此外,2个肾均出现肾小体萎缩、碱性物质沉积等病变。在血液学检测中,肾病蛇的红细胞和血栓细胞数量低于正常蛇,且有显著性差异。19项血液生化指标中有9项与正常蛇有明显差异。观察结果表明左肾的肥大可能是对右肾萎缩的补偿,其组织结构的变化也可能是一定程度上的功能反应。血液学指标的变化是由于这种形态异常引起的生理功能变化所致,说明这条蛇处于疾病状态。尽管对这种肾脏异常的诱因尚不清楚,但应对蛇的饲养管理、食物和营养状态予以足够重视。

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The balanced design method (BDM) upholds the concept of mixture performance balance, and provides a new direction in the field of mix design for enhancing the performance of hot-in-place recycled mixtures. In order to explore the effectiveness of BDM in the field of heat-in-place regeneration, BDM of heat-in-place regeneration was adopted to design the mix ratio of three kinds of heatregenerated asphalt mixtures with high content of reclaimed asphalt pavement(RAP). And compared with the Marshall design method (MDM), the road performance analysis was carried out. The optimal amount of recycling agent was determined based on rheological performance indicators, and the optimal asphalt content was determined through high and low temperature performance tests of asphalt mixtures. The applicability of two design methods in in-situ geothermal recycling technology was studied through multiple types of road performance tests. The results show that the optimal asphalt content of BDM is higher than that of MDM at each RAP content, the road performance of the balanced mixture can meet the requirements of the specification. Low temperature performance does not meet specification requirements. Although BDM weakens the high temperature performance of the mixture, the higher the RAP content, the lower the effect. And BDM can better ensure the low temperature, fatigue and water stability performance of the mixture than MDM, and significantly enhance the comprehensive performance of the hot-in-place recycled mixture. Therefore, BDM is more suitable for the hot-inplace regeneration technology than MDM. [ABSTRACT FROM AUTHOR]

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