In this study, three banana varieties, ‘Nantianhuang' ‘Zhongjiao 9' and ‘Hongxiangjiao' were studied as experimental materials to study the effects of sodium selenate solution on the plant growth, yield, fruit quality,contents of MDA, proline and selenium. The results were as follows:(1)The application of 0.25 and 0.50 g·plant-1 sodium selenate could significantly increase plant height of three banana varieties and the growth of basal stem circumference of ‘Nantianhuang' and ‘Hongxiangjiao', but not ‘Zhongjiao 9'.(2)The application of selenium had little effect on MDA content in leaves during vegetative growth, and MDA content increased or decreased significantly only in part time. The proline content in leaves of the three banana varieties were significantly reduced at 0.25 and 0.50 g·plant-1 sodium selenate. Selenium had a significant effect on selenium content in leaves. The higher selenium concentration, the higher the selenium content in leaves.(3)The application of selenium could significantly increase the yield of banana and the single fruit weight of ‘Zhongjiao 9' and ‘Hongxiangjiao'. The yield per plant and single fruit weight of ‘Zhongjiao 9' at 0.25 g·plant-1 sodium selenate were 24.38 kg and 165.86 g, which were 12.80% and 14.69% higher than those of the check(CK). The suitable concentration of sodium selenite could effectively increase the content of vitamin C and potassium in fruits.(4)The highest vitamin C contents of ‘Nantianhuang' ‘Zhongjiao 9' and ‘Hongxiangjiao' were 12.7, 13.9 and 10.6 mg·100g-1, which were 12.72%, 18.84% and 29.39% higher than CK. And the highest contents of potassium were 349, 279 and 397 mg·100g-1, which were 29.62%, 33.28% and 47.77% higher than CK.(5)The higher the concentration of selenium treatment, the higher the content of selenium in fruits. The selenium content of fruits in CK did not reach the standard of selenium enrichment. The three banana varieties reached the standard of selenium enrichment after treatment with 0.25 and 0.50 g·plant-1 sodium selenate. In conclusion, the application of sodium selenate solution in soil can increase the content of selenium in banana fruits, promote the plant growth, improve fruit quality of banana, and decrease the content of MDA and proline in leaves. However, there are differences in the effects of various banana varieties. These findings will provide a theoretical basis for the production and cultivation of selenium enriched banana. [ABSTRACT FROM AUTHOR]