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Human corneal endothelial cells (HCECs) are very important for maintaining corneal transparency, but HCECs remain arrested at the G1 phase after embryonic development and could not proliferate and regenerate in vivo. The density of HCECs decreases spontaneously with corneal development and aging, while systemic factors and corneal diseases can further cause a massive loss to HCECs, lead to corneal opacity and edema and ultimately induce vision impairment. Therefore, the regeneration of HCECs has always been a heated topic in the field of corneal endothelial research. Currently, function restoration of exogenous corneal endothelium mediated by cell therapy and autologous regeneration of endogenous HCECs have made amazing breakthroughs, with endogenous HCECs autologous regeneration being a more convenient and physiological treatment option. This review summarizes and analyzes the strategies and related techniques that are currently applied to the autologous regeneration of HCECs in aspects of operative treatment, gene therapy and pharmacological treatment. [ABSTRACT FROM AUTHOR]

International research essays on corrosion inhibitors from 2005 to 2020 from the Web of Science core collection were retrieved・ The development status and trend changes of corrosion inhibitors are obtained from research directions, international cooperation，institutional cooperation，co-citation analysis and keyword analysis using the analysis platform CiteSpace・ The analysis revealed that corrosion inhibitors are widely used in the fields of materials science and chemistry, China has the most number of articles (accounting for 21 ・ 04%), but the centrality is losser(0. 01); Morocco has a higher number of articles (accounting for 4.23%) and centrality (0. 23), indicating that its research depth and breadth, cooperation density with other countries，and radiation influence on corrosion inhibitors are prominent; Chinese Acad Sci, King Fahd University Petr & Minerals and North West University are the main research institutions, accounting for 2. 82%，2・ 19%, and 2. 19%，respective】y・ Their attributes belong to the university, indicating that academic institutions value and contribute to the field・ At present，corrosion inhib让ors are beginning to develop in the direction of high efficiency, non-toxicity, and environmental friendliness. [ABSTRACT FROM AUTHOR]

In order to explore the optimal feature combination of Sentinel-2 remote sensing image stand type classification, the classification and effect evaluation of broad-leaved forest, masson pine forest, fir forest and bamboo forest were realized. Selecting Changting County, Fujian Province as the study area, 10 original bands（O)were extracted from Sentinel-2 images, and 9 spectral indices（S）, 7 red-edge spectral indices（R）, 8 texture features（Te)were calculated, and 2 terrain feature indices（To)based on digital elevation data were calculated, for a total of 36 features. Using random forest algorithm to analyze the importance of different features in stand type classification, using out of band（OOB)data and average impurity reduction method to optimum individuality combination（OIC）. 6 different experimental protocols（O, O+To, O+To+S, O+To+S+R, O+To+S+R+Te, and OIC)were classified into stand types and the results were evaluated by confusion matrix. The results showed that the importance of the 36 features involved in the classification of stand types was 2.11%-5.43%, the altitude factor was the most important, and the mean and correlation of the red edge band, red edge spectral index, and texture features were also of high importance. Using the original band alone to classify the stand types, the classification accuracy was not high, the overall accuracy was 73.26%, and the Kappa coefficient was 0.64. Based on the original band, other features were introduced. Except the original band, other features can improve the classification accuracy. The optimum individuality combination（OIC)was the top 27 features of importance, including altitude, 8 original bands, 7 red-edge spectral indices, and 3 texture features, the classification accuracy was the highest, the overall accuracy was 83.13%, and the Kappa coefficient was 0.77, which was 0.82%-9.87% higher than the overall classification accuracy of the other five experimental schemes. Using Sentinel-2 images as the data source, the feature combination optimized by the random forest algorithm integrated the features that had an important contribution to the classification of stand types among the multi-type features, thereby improving the classification accuracy. The research results can provide reference for GEE platform Sentinel-2 image extraction of stand type information in forest resource survey. [ABSTRACT FROM AUTHOR]

Objective To observe the clinical characteristics of idiopathic osteoporosis among premenopausal women． Methods 28 idiopathic osteoporosis patients，15 secondary osteoporosis patients and 56 normal cases were selected from 1043 premenopausal women according to the inclusion criteria in Shanghai First People’s hospital between September 2005 and December 2016． Subjects were divided into three groups: idiopathic osteoporosis group，secondary osteoporosis group and control group． Ｒetrospective statistical analyses were performed for basic clinical characteristics，bone turnover markers，bone mineral density ( BMD) and fracture data． Ｒesults BMD of the lumbar spine，femoral neck and hip in the idiopathic osteoporosis group were significantly lower than that of the control group ( t = － 3. 794，P ＜ 0. 01; t = － 4. 080，P ＜ 0. 01; t = － 5. 632，P ＜ 0. 01) ． There were no significant differences in the levels of calcium，phosphorus and vitamin D between the idiopathic osteoporosis group and the control group ( t = 0. 120，P ＞ 0. 05; t = 0. 121，P ＞ 0. 05; t = 0. 004，P ＞ 0. 05) ． There was a trend of lower levels of OC and β-CTX in the idiopathic osteoporosis group than that of the control group，but the differences were not statistically significant ( t = 1. 605，P ＞ 0. 05; t = 1. 543，P ＞ 0. 05) ． BMD of the lumbar spine in the secondary osteoporosis group was significantly lower than that in the idiopathic osteoporosis group ( P = 0. 035) ． However，there were no significant differences in BMD of the femoral neck and hip between the secondary osteoporosis group and the idiopathic osteoporosis group ( P = 0. 298，0. 223) ． The levels of OC and β-CTX in the secondary osteoporosis group were higher than that in control group ( P = 0. 020，P ＜ 0. 01 ) ． There was no significant difference in the level of OC between the secondary osteoporosis group and the idiopathic osteoporosis group ( P ＞ 0. 05) ． The level of β-CTX in the secondary osteoporosis group was higher than that in the idiopathic osteoporosis group ( P ＜ 0. 01) ． Conclusion The pathogenesis of idiopathic osteoporosis in premenopausal women may not be related to the levels of Ca，P and 25OHD，and the high turnover of bone metabolism might be the main cause of the disease．11 [ABSTRACT FROM AUTHOR]

This experiment is combined with the project of National Natural Science Foundation of innovation team in College of Tianjin City, to update the biotechnology and related professional’s comprehensive experimental project as the breakthrough point, to the primer of CRISPR locus in the genome of Arthrospira sp. Taking TJSD091 as an example, the paper elaborates how to determine the optimal annealing temperature in PCR amplification reaction by the combination of Touchdown PCR and gradient PCR. The reform stimulates students’ inquiry psychology and study interests, guides students to take the initiative to learn. The advantages of combining scientific research with experimental teaching are analyzed, it is believed that this teaching mode can train students’ scientific thinking, improve students' ability to solve practical problems, and can provide a new way for the improvement of the practical teaching system for the undergraduates in applied colleges. [ABSTRACT FROM AUTHOR]

钾通道是一个种类繁多、结构复杂、分布广泛的多样化蛋白家族,在多种血管内皮细胞和平滑肌细胞的生理活动中发挥着重要作用.目前随着"离子通道病"研究的日益深入,胎盘血管钾通道与某些妊娠并发症的关系也逐渐引起了人们的关注.本文通过综述相关研究进展,为钾通道对胎盘血管张力的调控作用提供可能依据,从而更好地阐明胎盘血管相关异常妊娠的病理生理机制. [ABSTRACT FROM AUTHOR]

[Objectives] In order to clarify the difference in activation of the immune system by live or inactivated viruses after entering the body, a high-efficiency animal model was established for the evaluation of the efficacy of immune enhancers to better improve the immune efficacy of inactivated vaccines. In this experiment, the Pseudorabies virus (PRV) Bartha-K61 strain was used to establish a PRV artificial infection mouse model, and to screen the immune-related difference factor after inoculation with live or inactivated antigens. [Methods]PRV Bartha-K61 was inoculated to experimental mice in different doses and methods, and the survival rate was calculated to screen the safe dose. Mice were vaccinated with live virus or inactivated virus at a safe dose, and ELISA kits were used to detect the level of serum PRV-specific gB antibodies and the level of serum TNF-α, IL-1β, IFN-β and IL-6 of mice 7 days after immunization; inactivated PRV was used as a specific antigen to perform lymphocyte proliferation experiments and calculate the lymphocyte proliferation index(stimulation index, SI)of each group to screen the best vaccination route. [Results] 5×104 TCID50 was a safe challenge dose for 6-8 weeks mouse; subcutaneous injection was the best way of infection. Compared with the live virus and the inactivated virus group after 7 days of infection, the serum levels of TNF-α, IL-1β and IFN-β significantly increased (P<0.001, P<0.01), the levels of PRV gB IgG antibody and the lymphocyte SI in the mouse serum also significantly increased (P<0.05, P<0.001); There is no significant difference in cytokines and SI between different inactivation methods (P>0.05). [Conclusions]The experiment successfully established an artificial pseudorabies virus infection model in mice, and clarified that after live or inactivated pseudorabies virus immunized mice, there were differential immune-related factors in the early stage of the body, providing a research platform for the evaluation and screening of subsequent immunopotentiator. [ABSTRACT FROM AUTHOR]

[Objectives] This study aimed at deciphering the mechanisms of CVC1302 in promoting germinal center(GC) responses. [Methods]Pattern antigen 4-hydroxy-3-nitrophenylacetyl(NP)-ovalbumin(OVA)were mixed with CVC1302 followed by emulsified with ISA206-named as NP-CVC1302. Six-week-old BALB/ c femal mice were immunized with NP-CVC1302-NP or ISA206-and the sera were collected to detect the NP-specific antibody titers and isotype antibody titers. The percentages of follicular helper T cells (TFH )-NP + GC B cells and NP + plasma-blasts in inguinal lymph nodes and the numbers of GC were measured at 14 days post immunization(dpi). Furthermore-the percentage of NP + long-lived plasma cells(LLPC)in bone marrow was also detected at 42 dpi by using flow cytometry. [Results] Through the detection-we found that NP-specific antibody titers were significantly higher in NP-CVC1302 group compared with NP group(P< 0.01)-as well as the NP-specific IgG1 and IgG2a antibody titers. Significantly higher percentages of TFH cells-NP + GC B cells and NP + plasma-blasts in inguinal lymph nodes were induced in NP-CVC1302 group compared with NP group(P<0.01 or P<0.001). Moreover-significantly higher percentage of LLPC was transferred into the bone marrow of mice that received NP-CVC1302 compared with those that received NP(P< 0.01). [Conclusions] Results of the study revealed that CVC1302 improved the differentiation of TFH cells-then TFH cells selected the high-affinity GC B cells to differentiate into plasma-blasts-and finally became LLPC locating in the bone marrow-continuing to secrete antibody. This study provided the blueprint for designing new generations of immunopotentiators. [ABSTRACT FROM AUTHOR]

目的 观察多发性硬化症（MS）患者肺功能指标及呼吸中枢驱动功能的变化，探讨疾病对患者呼吸系统功能的影响。 方法 选取2012年3月至2015年12月在北京中关村医院神经内科和西南大学医院康复理疗住院的MS患者30例作为MS组，另选取同期健康体检者30例作为对照组，对两组研究对象进行肺功能和呼吸中枢驱动功能测定，分析两组研究对象各指标的差异。 结果 MS组患者用力肺活量、用力1秒呼气量、呼气流速峰值、最大吸气压力（PImax）、最大通气时的呼吸中枢驱动力（P0.1max）、每分钟通气量（MV）校正呼吸中枢驱动力均显著低于对照组，潮气量和MV、PImax校正呼吸中枢驱动力、气道阻断压力（P0.1）/P0.1max显著高于对照组，差异均有统计学意义（P<0.05）；用力1秒呼气量占预计值百分比、气道阻力、最大呼气压力与对照组比较，差异均无统计学意义（P＞0.05）。MS组P0.1略低于对照组，但差异无统计学意义（P＞0.05）。 结论 MS患者吸气及呼气功能均受累，呼吸中枢驱动力及储备下降。 [ABSTRACT FROM AUTHOR]

[Objectives]The purpose of this study is to verify the feasibility of purification of type O foot and mouth disease virus(FMDV) inactivated antigen with Gram-positive bacterial enhancer matrix (GEM) technology. [Methods]A dual functional molecular consisting of anchor protein gene and FMDV specific single domain antibody gene of type O FMDV were expressed in Escherichia coli,and the recombinant protein was named as VPA. The sedimentation named GEM-VPA was obtained with centrifugation following mixing the recombinant protein with GEM particles,and incubated at 37℃ for 30 min;GEM-VPA was mixed with FMDV antigen,and then the sedimentation was collected with low speed centrifugation after incubated at 37℃ for another 1 h,which was the purified antigen named GEM-FMDV. The feasibility for the method was evaluated with SDS-PAGE,Western-blot,protein quantification and 146S detection,and the immunogenicity for purified FMDV antigen was estimated with animal test. [Results]The dual functional protein designed in this study could be partly soluble when expressed in Escherichia coli. Moreever,this recombinant protein could bind with GEM particles and inactivated FMDV antigen at the same time. SDS-PAGE and Western-blot proved that the purified FMDV antigen existed in the sedimentation after centrifugation. Results from 146S determination indicated that the FMDV antigen recovery in this study could be above 99%,and the removal efficiency of the heterologous proteins from FMDV antigen was higher than 90%. More importantly,animals injected with the purified antigen developed more vigorous immune response and lesser side effect. [Conclusions]It is feasible for FMDV antigen concentration and purification with GEM technology. [ABSTRACT FROM AUTHOR]

[Objectives] The objective of this project is to determined whether the recombinant fluorescent antibody expressed in Escherichia coli can be used to detect Classical swine fever virus(CSFV) in direct immune fluorescence assay. [Methods] The gene VHH-EGFP synthesized according to condon usage of E.coli was cloned into pET32a (+) and then transformed into E.coli BL21(DE3). The recombinant cells were cultured in LB (containing 50 μg·mL-1 of ampicillin) and incubated in 37 °C until A600=0.6-0.8, then the target gene was induced with 0.4 mmol·L-1 IPTG for 24 h at 15 °C. The induced bacterial cell pellet was collected and cracked, the interest protein was identified by 120 g·L-1 SDS-PAGE and Western blot, after confirmation, the expressed target protein was purified with Ni-NTA His·Bind Resin and detemined by BCA protein assay kit, and then it was incubated with cells infected with CSFV, PCV2, PRV, PPV to analyze its specificity and sensitivity. [Results] SDS-PAGE and Western-blot indicated that the recombinant fluorescent antibody was successfully expressed in E.coli BL21(DE3). Although it was mainly expressed as inclusion body form, the soluble protein could be purified and concentrated by nickel column. The PK15 cells infected with CSFV showed numerous obvious green fluorescences points after it was incubated with recombinant antibody. However, there was no or only few nonspecific fluorescence when the recombinant protein incubates with cells infected with PCV2, PRV, PPV. Compared with the IFA based on the commercial monoclonal antibody, the DFA constructed in this study had more sensibility and accuracy. [Conclusions] The optimized VHH-EGFP gene could be efficiently expressed in E.coli BL21(DE3) system, and the purified recombinant fluorescent antibody was more effective comparable to the commercial monoclonal antibody, and it can be produced with a cheaper and easier process. [ABSTRACT FROM AUTHOR]

【Objective】This experiment was conducted to study on excretion of feed zinc in feces and urine of growing pigs and establish the prediction model of zinc intake and zinc excretion.【Method】A total of 20 healthy castrated Yorkshire growing pigs with similar weight were randomly allotted to 5 dietary treatments with 4 replicates in each treatment and 1 pig in each replicate, animals were individually housed in cages. In 5 treatments, control group A was fed a basal diet without zinc supplementation, test group B, C, D, E were fed basal diet supplemented with 20, 40, 60 and 80 mg/kg zinc as zinc sulfate, respectively. The pre-test period lasted for 7 days, and the experimental period lasted for 4 days.【Result】(i) The fresh fecal zinc content and air-dry fecal zinc content in experimental groups were higher than that in control group, with feed zinc supplementation levels being increased, both tended to be higher in the experimental groups. Fresh fecal zinc content, air-dry fecal zinc content and daily fecal zinc excretion in experimental group C and D were significantly higher than that in control group(P<0.05). Regression analysis demonstrated that there was extremely significant linear regression relationship between feed zinc supplementation levels or feed zinc total levels and air-dry fecal content(P<0.01), in addition, there was significant linear regression relationship between feed zinc supplementation levels or feed zinc total levels and daily fecal zinc excretion(P<0.05). Therefore, regression equations could be established for prediction and control.(ii) There were no significant differences in urinary zinc content and daily urinary zinc excretion among all groups(P>0.05). Regression analysis demonstrated that there was no significant regression relationship between feed zinc supplementation levels or feed zinc total levels and urinary zinc content or daily urinary zinc excretion(P>0.05).【Conclusion】 Under the condition of this trial, there was significant linear regression relationship between feed zinc supplementation levels or feed zinc total levels and air-dry fecal content or daily fecal zinc excretion, while there was no significant regression relationship between feed zinc supplementation levels or feed zinc total levels and urinary zinc content or daily urinary zinc excretion, because feed excess zinc was excreted mainly from feces though digestive tract. Therefore, fecal zinc excretion could be predicted based on feed zinc levels, so that mankind could control the pollution of zinc to environment from the source. [ABSTRACT FROM AUTHOR]

[Objectives]The paper aimed to detect microRNA(miRNA)expression profile in Japanese encephalitis virus(JEV)infected swine testis(ST)cells.[Methods]Total RNA of JEV-infected and uninfected ST cells was extracted individually by Trizol and then analyzed miRNA expression profiles by high-throughput sequencing technology. miRNA target prediction algorithm miRanda was employed to predict potential target genes of differently expressed miRNA. Gene ontology(GO)analysis was performed on the host target genes,and 6 signifacant differences in expression of miRNA were verified by quantitative real time polymerase chain reaction(RT-qPCR).[Results]The results indicated that total 112 miRNA were obviously differently expressed in the JEV-infected cells including 80 up-regulated miRNA and 32 down-regulated miRNA. The six of those miRNA were verified by RT-qPCR,the results were well matched with those of sequencing data. GO analysis of the target genes showed that these miRNA involved in the biological processes of cell metabolism and cell adhesion.[Conclusions]Our findings indicate that miRNA may play critical roles in the pathogenesis of JEV infection. [ABSTRACT FROM AUTHOR]

Objective: To investigate the correlation between body mass index, endocrine and metabolic markers in patients with polycystic ovary syndrome. Methods: 53 patients who suffered polycystic ovary syndrome from October 2016 to July 2017 were grouped according to BMI, HOMA-IR and testosterone levels. Body weight index(BMI), blood biochemistry, insulin, C peptide and sex hormone and endocrine and metabolic indicators were tested and analyzed. Results: Grouped according to the level of BMI, there were statistical significance in fasting insulin, 60 minutes insulin, 120 minutes of insulin, fasting C peptide, 60 minutes C peptide, 120 minutes C peptide,total cholesterol, triglyceride, low density lipoprotein, high density lipoprotein(P<0.05); Grouped according to the level of the HOMA-IR index, there were statistical significance in fasting blood glucose, 60 minutes blood glucose, 120 minutes blood glucose, fasting insulin,60 minutes insulin, 120 minutes insulin, fasting C peptide, 60 minutes C peptide, 120 minutes C peptide, total cholesterol, triglyceride,low density lipoprotein, high density lipoprotein, LH/FSH, testosterone and estradiol(P<0.05); grouped according to testosterone, there were statistical significance in BMI, fasting insulin, 60 minutes insulin, 120 minutes of insulin, HOMA index, luteinizing hormone, LH/FSH, testosterone and estradiol(P<0.05). Conclusion: Polycystic ovary syndrome(PCOS) is complicated and changeable. It should be treated differently according to the body mass index, endocrine and metabolic characteristics, so as to improve the cure level and quality of life. [ABSTRACT FROM AUTHOR]

【Objective】The experiments were conducted to evaluate the effects of fermented rapeseed meal on the performance, immunology and antioxidant function of growing pigs. 【Method】A total of 64 healthy crossbred (Duroc x Landrace x Yorkshire) growing pigs with similar weight were randomly allotted to 4 dietary treatments with 4 replicates in each treatment and 4 pigs in each replicate. In 4 treatments, control group was corn-soybean basal diet, three experimental groups were fermented rapeseed meal replaced soybean meal in the basal diet at 2. 5 %, 5 %, 7. 5 % 'respectively! each dietary treatment was isonitrogenous). 【Result】(i) The average daily gain (ADG) and average daily feed intake ( ADFI) in experimental groups were higher than that in control group. ADG, ADFI in group 3 and ADFI in group 2 were significantly higher than that in control group(P < 0. 05). There were no significant differences in ratio of feed to gain (F/G) among all treatment groups, but F/G in group 3 was the minimum, 6 . 07 % lower than the control group (P > 0. 05). (ii) The serum superoxide dismutase(SOD) activity and total antioxidant capability(T-AOC) in group 3 were significantly higher than that in control group(P < 0. 05), the serum malondialdehyde(MDA) content was significantly lower than that in control group(P < 0. 05 ). There were no significant differences in serum glutathione peroxidase (GSH-Px) and catalase(CAT) activity among all groups (P > 0. 05). (iii) The serum immunoglobulin A( IgA) ,immunoglobulin G(IgG) and interleukin-2(IL-2) contents in group 3 were significantly higher than that in control group(P < 0. 05). There were no significant differences in serum immunoglobulin M(IgM), interleukin-1 ( IL-1), interleukin-6 ( IL-6 ) and tumour necrosis factor-) ( TNF-a) contents among all groups (P > 0. 05). 【Conclusion 】In conclusion， under the condition of this trial， the fermented rapeseed meal can improve the performance，antioxidant and immunology function of growing pigs，and its optimal replacement level was 7.5%. [ABSTRACT FROM AUTHOR]

miRNAs are endogenous non-coding RNAs about 22 nucleotides in length that play an important role in posttranscriptional regulation, also play an important role in physiological and pathological.So far, there have been found 24521 miRNA molecules in animals and plants as well as the virus, miR-378 is one of the family of miRNAs.miR-378 is closely related to the occurrence and development of many diseases through a variety of mechanisms.In different tissues, miR-378 plays different roles.miR-378 plays the role of tumor suppressor genes in gastric cancer, liver cancer and colorectal cancer.miR-378 has the opposite effect on ovarian cancer, pancreatic cancer and leukemia.In the field of cardiovascular diseases, miR-378 can protect blood vessel, delay cardiovascular disease development by a variety of mechanisms.In bone metabolism, miR-378 can inhibit or promote osteoblast differentiation through different mechanisms.This paper will introduce the relationships between miR-378 and tumors, cardiovascular, bone metabolism and other aspects, in order to provide a new idea for the treatment and prevention of these diseases. [ABSTRACT FROM AUTHOR]