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Aspergillus niger is an important strain for industrial production of organic acids and enzymes, possessing a powerful hydrolytic enzyme system and glucose transport system, which enable to copy with extracellular carbon sources variance and uptake glucose to support cell growth and industrial production. As an important carbon source and an essential signaling molecule, glucose and its uptake has considerable influence on cell growth and fermentation performance. Regarding to issue of lacking simple methods for quantitative characterization of glucose absorptive capacity in filamentous fungi, we developed a quantitative detection pipeline for glucose uptake capacity assay for filamentous fungi, using a non-metabolizable,flourscent glucose analog, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-yl)amino)-2-deoxyglucose(2-nbdg) as a fluorescent probe, then incubating pre‑cultured A. niger spores with 2‑NBDG, and using with fluorescence microscopy and flow cytometry. It showed that the optimized 2‑NBDG work concentration and incubation time were 150 μmol/L and 4 h, respectively. Using this quantitative analysis method, it was found that the overexpression of low affinity glucose transporter MstC increased glucose uptake by 1.44 times. Meanwhile, on the basis of previous studies, mutant R188K of MstC was designed, and the point mutation directly caused the loss of glucose transport activity of MstC, which indicates that Arg188 is a key amino acid site affecting the glucose transport capacity of MstC. The establishment of this quantitative glucose uptake detection method and its application to the study of MstC function not only deepens the quantitative understanding of glucose absorption in filamentous fungi, but also provides technical support for the transformation and optimization of glucose transport system. This study provides a feasible quantitative approach to investigate the glucose uptake of filamentous fungi, and also revealed the physiological function of key glucose transporter MstC, which paves the way for glucose uptake engineering in this industrially important fungal cell factory [ABSTRACT FROM AUTHOR]

Objective The aim is to explore the impact of fracturing construction parameters on the erosion of movable elbow, and movable elbow erosion behavior and change trend of maximum erosion rate under different working conditions. Methods The CFD software is used to establish a model of double-curved movable elbow with a diameter of 69.85 mm, and the erosion behavior of the movable elbow under different installation angles, proppant mass flow rates, fracturing fluid flow rates and viscosity conditions is simulated and calculated in combination with the actual fracturing conditions. Results Under different working conditions, the maximum erosion rate of the elbow occurs at the outlet of the outer arch side of the second curved arc. Under the same condition of other parameters, the maximum erosion rate of the movable elbow appears a minimum when the installation angle is 75°. When the mass flow rate of proppant is in the range of 4.2-25.2 kg/s, the maximum erosion rate increases linearly. When the mass flow rate of proppant reaches 33.6 kg/s, the erosion rate decrease slightly due to the "shielding effect". Erosion rate shows an approximate exponential growth trend with the increase of fracturing fluid flow rate; When the viscosity of the fracturing fluid is within the range of 10-40 mPa · s, the maximum erosion rate of the movable elbow is relatively stable, and the maximum erosion rate also increases with the increase of the viscosity, and tends to be stable after the viscosity reaches 120 mPa · s. Conclusions The research results are basically consistent with the field practice, which can provide effective reference for the layout of moveable elbow and the optimization of construction parameters in the fracturing field. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of desloratadine citrate disodium combined with endoscopic sinus surgery on clinical symptoms and inflammatory response in patients with chronic sinusitis. Methods: 116 patients with chronic sinusitis admitted to our hospital from March 2017 to July 2018 were selected as the research objects. They were divided into traditional group and combined group according to random number table method, 58 cases in each group. The traditional group were treated with endoscopic sinus surgery. The combined group were treated with desloratadine citrate disodium on the basis of endoscopic sinus surgery. The clinical efficacy, symptom visual analogue scale (VAS) scores, clearance function of nasal mucociliary, levels of inflammatory factors and adverse reactions were compared between the two groups. Results: The total effective rate of the combined group were higher than that of the traditional group, and the VAS scores of headache, nasal obstruction, facial pain and olfactory disturbance in the combined group were lower than those in the traditional group (P<0.05). The clearance rate and clearance speed of the two groups after treatment were higher than those before treatment, and the clearance time was shorter than that before treatment (P<0.05). Compared with the traditional group, the clearance rate and clearance speed of the combined group increased and clearance time shortened after treatment (P<0.05). After treatment, the levels of tumor necrosis factor-α (TNF-α), interleukin-5 (IL-5), interleukin-12 (IL-12) and high-sensitivity C-reactive protein (hs-CRP) in the two groups were lower than those before treatment, and those in the combined group were lower than those in the traditional group(P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: On the basis of endoscopic sinus surgery, combined use of desloratadine citrate in the treatment of chronic sinusitis patients has a definite effect, effectively alleviating inflammatory response, alleviating the clinical symptoms of patients. [ABSTRACT FROM AUTHOR]

Although activities of daily living (ADL) is an important indicator of the health status of the elderly, the rigorous quantitative studies of the relation hip between it and the residual life has been well studied. According to t he panel survey data of the elderly aged from 55 upward from China health and nutrition survey, we constructed a stochastic filtering model to predict the residual life based on ADL. Then, the goodness-of-fit test was carried out to test the model. The resuIt demonstrates that the ADL which is closely related to health status can be well used in residual life prediction when biochemical markers are not available in most cases. It provides a scientific basis for paying attention to the ADL of the elderly which is advocated by population health management and an important reference for determining premiums by life insurance companies through the ADL of the elderly. [ABSTRACT FROM AUTHOR]

目的:探讨经肘关节肱三头肌内外侧双切口手术治疗儿童肱骨髁上GartlandⅢ型骨折的疗效。方法选取2014年4月至2015年3月该科收治的儿童肱骨髁上骨折患儿256例，其中103例GartlandⅢ型骨折采用手法复位小夹板外固定治疗失败，紧迫转行经肘关节肱三头肌内外侧双切口切开复位克氏针交叉内固定治疗。结果该组103例患儿均获随访，随访时间2~9个月，平均4个月。103例均愈合，骨折愈合时间1.5~8.0个月，平均2个月。按Flynn功能评定标准:优76例，良17例，可8例，差2例，优良率90.3%。结论经肘关节肱三头肌内外侧双切口手术治疗难复性儿童肱骨髁上GartlandⅢ型骨折具有复位精准，固定安全可靠，肘内翻发生率低，手术创伤小，不破坏伸肘装置，紧迫手术肢体肿胀消退快，可早期功能锻炼，术后功能恢复较好，是较理想的手术方式。 [ABSTRACT FROM AUTHOR]

The shielding effect of urban buildings under the action of wind field directly affects the process of precipitation runoff. To improve the accuracy of urban stormwater simulation, it is necessary to uncover the unique runoff characteristics of built- up areas under the action of wind field and establish corresponding calculation methods. The runoff surface of built-up areas was divided into horizontal and vertical runoff surfaces, and the rainfall inclination angle caused by the wind field was calculated based on computational fluid dynamics theory. Geometric relationships were integrated to determine the distribution of inclined rainfall on the horizontal and vertical runoff surfaces. Based on the runoff theory, the runoff of the two types of runoff surfaces was calculated and formed the runoff calculation method of built-up areas. The method was coupled into the storm water management model (SWMM) and verified in the experimental watershed. The results showed that: ① the modified SWMM had higher Nash model efficiency coefficient than the original SWMM, and simulated flood peak better; ② the values of some key parameters, such as soil saturated conductivity, obtained via the modified model were more appropriate; ③ the modified model was more stable for runoff simulation of built- up areas under different meteorological conditions. Therefore, this method can improve the simulation accuracy of runoff in built- up areas [ABSTRACT FROM AUTHOR]

Objective To observe the effects of Tongluo Zhitong Ningjiao (Collateral-unblocking and Pain-relieving Gel, TLZTNJ) on the synovial membrane inflammation severity in rabbit models of knee osteoarthritis (KOA) at different stages, and explore its mechanism in the treatment of KOA. Methods 56 rabbits were randomly divided into 8 groups: normal group, sham operation group, mild KOA group, moderate KOA group, severe KOA group, mild KOA group treated with TLZTNJ, moderate KOA group treated with TLZTNJ, and severe KOA group treated with TLZTNJ. Except for the normal group and the sham operation group, KOA models in the other groups were established with the Hulth method. The mild KOA group, the moderate KOA group, and the severe KOA group were executed in the 4th, 8th and 16th week respectively after the models were established. The mild, moderate, and severe KOA groups treated with TLZTNJ were treated with the gel for 3 weeks after the mild, moderate, and severe KOA groups were executed and were executed too. The degree of synovial inflammation was observed by HE staining. The levels of IL-1 β, IL-6, TNF-α and PGE2 in serum were detected by ELISA. The mRNA and protein expressions of IL-1 β, TNF-α and MMP-13 in synovial tissue were detected by qPCR and WB. Results The synovial layer of rabbits in the mild, moderate, and severe KO A groups significantly thickened, and the inflammatory infiltration increased. After the intervention with TLZTNJ, the severity of synovial inflammation in the mild, moderate, and severe KO A rabbit groups treated with TLZTNJ were significantly reduced. TLZTNJ lowered the levels of IL-1 β, IL-6, TNF-α and PGE2 in the serum (P < 0. 05, P <0. 01) and inhibited the expression levels of IL-1 β, TNF-α and MMP-13 (P < 0. 05, P <0. 01) of the rabbits with mild, moderate, and severe KO A. Conclusian TLZTNJ can significantly inhibit synovial membrane inflammation and reduce the expression of inflammatory factors in rabbit KO A models at different stages. [ABSTRACT FROM AUTHOR]