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[目的]研究人类免疫缺陷病毒（HIV）-1 nef调节细胞表面的人类白细胞抗原（HLA）-Ⅰ类分子及诱导细胞凋亡情况。[方法]将293T细胞分为两组,用脂质体法将含HIV-1 nef基因的质粒及空载体分别转染细胞,Western blot检测HIV-1 nef在转染细胞中的表达,荧光抗体染色结合流式细胞术检测细胞表面的HLA-I类分子表达及细胞凋亡情况。[结果]HIV-1 nef在转染细胞中有表达。转染HIV-1 nef的细胞与转染空载体的细胞经抗HLA-A,B,C-FITC染色后,平均荧光强度分别为205±22及269±15,两者相比有显著性差异（P〈0.01）;经Annexin V-APC/PI染色后,阳性细胞百分率分别为（60.82±8.32）%及（8.12±5.43）%,两者相比亦有显著性差异（P〈0.01）。[结论]HIV-1 nef下调细胞表面的HLA-I类分子及诱导细胞凋亡。

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Objective: To investigate the expression inhibition and correlation of indoleamine 2, 3-dioxygenase （IDO） and cytokine signal transduction inhibitor protein-3 （SOCS3） in placenta of patients with hypertensive disorder complicating pregnancy （HDP）. Methods: The expressions of IDO and SOCS3 in placenta of HDP were detected by Western blot method, and the expressions and correlation of them in placenta were analyzed. Results: IDO and SOCS3 were expressed in placenta of patients with HDP and normal pregnancy, and their expression was negatively correlated with the progression of HDP. There was a positive correlation between the expressions of IDO and SOCS3 in placenta of the three groups, and the correlation in preeclampsia group was higher than that in HDP group and normal pregnancy group, and the correlation in HDP group was significantly higher than that in normal pregnancy group. Conclusion: IDO and SOCS3 play an important role in immune tolerance in the third trimester of pregnancy, and may be involved in the pathogenesis of HDP. IDO and SOCS3 expressions and the degree of correlation can pre-reveal the progression of HPD. [ABSTRACT FROM AUTHOR]

Objective To compare the efficacy and safety of domestic and imported recombinant human follicle stimulating hormone (rFSH) preparation for ovulation induction in non-low-response population with long-acting program in follicular phase. Methods Non-ovarian infertility patients with low response were retrospectively enrolled in our center for the first time to receive long-acting follicular phase IVF/ICSI assisted fertility treatment, all of whom received a COS long-acting follicular phase therapy, and were divided into the domestic rFSH group (n=267) and the imported rFSH group (n=240) according to different use of rFSH. Ovarian response, medication, egg fertilization and embryo, fresh embryo transfer and embryo thawing transfer were compared between the two groups. Results During the process of superovulation, the level of E2 on HCG day was lower in the domestic rFSH group than that in the imported rFSH group (P＜0.05). There were no significant differences in total Gn, days of Gn use, the number of MⅡ eggs, the number of D3 excellent embryos and number of high-quality blastocysts between the two groups (P＞0.05). In the primary efficacy indicators, there were no significant differences in the average number of egg retrieved, cumulative live birth rate of one COS number of fresh embryos, the clinical pregnancy rate in the transplantation cycle, and the clinical pregnancy rate in the freeze-thaw transplantation cycle between the two groups (P＞0.05). Conclusion Compared with imported rFSH in the follicular phase, domestic rFSH can achieve the same safe efficacy and clinical outcome in non-low response patients. [ABSTRACT FROM AUTHOR]