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3 results on '"3D fibrin scaffold"'

1. 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton’s Jelly Mesenchymal Stem Cells

Author

Maria Camilla Ciardulli, Joseph Lovecchio, Pasqualina Scala, Erwin Pavel Lamparelli, Tina Patricia Dale, Valentina Giudice, Emanuele Giordano, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, and Giovanna Della Porta

Subjects
human Wharton’s Jelly Mesenchymal Stem Cells, hGDF-5 controlled delivery, PLGA nanocarriers, 3D fibrin scaffold, tenogenic commitment, cyclic strain bioreactor systems, Pharmacy and materia medica, RS1-441
Abstract

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

Published
2021
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2. 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An in-vitro study of Tenogenic events on Wharton’s Jelly Mesenchymal stem cells

Author

Giovanna Della Porta, Nicola Maffulli, Pasqualina Scala, Joseph Lovecchio, Valentina Giudice, Tina P. Dale, Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Nicholas R. Forsyth, Emanuele Giordano, Carmine Selleri, Ciardulli M.C., Lovecchio J., Scala P., Lamparelli E.P., Dale T.P., Giudice V., Giordano E., Selleri C., Forsyth N.R., Maffulli N., and Porta G.D.

Subjects
3D fibrin scaffold, Cyclic strain bioreactor systems, HGDF-5 controlled delivery, Human Wharton’s Jelly Mesenchymal Stem Cells, PLGA nanocarriers, Tenogenic commitment, Decorin, medicine.medical_treatment, Pharmaceutical Science, Article, RS, Pharmacy and materia medica, Wharton's jelly, medicine, Chemistry, Growth factor, Mesenchymal stem cell, Scleraxis, Growth differentiation factor, Human Wharton’s Jelly Mesenchymal Stem Cell, Cell biology, RS1-441, PLGA nanocarrier, Nanocarriers, Type I collagen, Cyclic strain bioreactor system
Abstract

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14, on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

Published
2021

3. 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells.

Author

Ciardulli, Maria Camilla, Lovecchio, Joseph, Scala, Pasqualina, Lamparelli, Erwin Pavel, Dale, Tina Patricia, Giudice, Valentina, Giordano, Emanuele, Selleri, Carmine, Forsyth, Nicholas Robert, Maffulli, Nicola, and Della Porta, Giovanna

Subjects
*MESENCHYMAL stem cells, *GROWTH differentiation factors, *DRUG delivery devices, *HUMAN stem cells, *JELLY, *TRANSFORMING growth factors, *TISSUE scaffolds, *GROWTH factors
Abstract

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton's Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

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