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In the article, the authors presented the results of comprehensive studies in 2020 - 2022. on the study of different varieties of soybeans when grown up to granules. The experiments were carried out on heavy loamy grey forest soil with a humus content of 4.1% with a slight acid reaction of the medium (pH 6.28). The nitrate nitrogen concentration was 10 mg/100 g, mobile phosphorus 13.5 mg/100 g, and exchangeable potassium 8 mg/100 g. Different varieties of soybeans, according to their ecological and geographical origin, were sown in the third decade of May with a sowing rate of 500 thousand viable seeds per 1 ha, with harvesting in the third decade of September. The authors conducted phenological observations and determined the photosynthetic potential during the experimental work. The authors also determined the leaf areas of different soybean varieties, plant height, yield indicators and crop structure. The researchers noted that the accelerated growth and development rates occurred in the West Siberian types Omskaya 4, SibNIIK-315, Gorinskaya and Krasnoobskaya. Early maturing varieties of Far Eastern selection were distinguished by a long growing season of up to 120 days, with 85 days in Siberian varieties: Omskaya 4, Far Eastern selection Alena, and Kuban selection (Barguzin, Puma, Vita, Lira, Sayana). The authors observed a significant increase in yield to the standard in the Omskaya 4 - 21% variety. On the other hand, types of the Far Eastern and Kuban selection showed grain yield indicators 2–5 times lower than the SibNIIK-315 standard. The high parameters of the crop structure in the Siberian variety Omskaya 4 were determined by the number of beans from one plant and the mass of grains from one plant.

The authors in the article provided data on the use of different varieties and hybrids of cereals and legumes. The experiments were carried out in the forest steppe of the Novosibirsk Priobya region in 2020–2022. The studies were conducted on the leached chernozem of the Praktik EEF (Educational and Experimental Farm) of the Novosibirsk Region and CJSC Livestock Breeding Farm Irmen of the Ordynsky District Novosibirsk Region. Also, the authors conducted experiments in the steppe zone on the southern chernozem of Nadezhda LLC of the Bagansky district and on grey forest medium loamy soil of the Education and Production Farm (EPF) “Garden of Michurintsev” in Novosibirsk. The research aims to study the efficiency of using the modern gene pool of grain and leguminous crops in different zones of Western Siberia. The authors found that West Siberian selection soybean varieties had higher growth and development rates than the Doka standard for 4-6 days. During studies with F1 hybrids of corn, the authors also note increased indicators of biometric parameters: plant height, green mass, and cob weight - by 20% of the Ross 140 F1 standard. High yield parameters of corn grain on the leached chernozem of the forest-steppe zone (EEF Praktik) of hybrids Ross 199 F1 - 10.2, KS-178 F1 and Krasnodar 194 F1 - 9.79 t/ha were revealed, exceeding the Ross 140 F1 standard by 40– 47%. Corn grains of hybrids F1 Ross 199 - 6.03 t/ha, Krasnodar 194 - 5.67 and KS-178 - 5.52 t/ha differed in yield with an excess of the standard by 54% on the southern chernozem of the steppe zone of Nadezhda LLC The maximum yield of corn grain is in the hybrid F1 Kuban 101 and Katerina - at 9 t/ha on the leached chernozem of CJSC Irmen stud farm. The maximum yield of soybean grain is 3.44 t/ha for SibNIIK-9 and Gorinskaya on leached chernozem, which is higher than the standard for Doka by 67 and 57%. SibNIIK-9, Omskaya 4, and Gorinskaya were distinguished on grey forest medium loamy soil. The variety of the Far Eastern selection Alena and the early ripening varieties of the RRIO collection (All-Russian Research Institute of Oilseeds, named after V. S. Pustovoit, Krasnodar): Barguzin, Lira, Sonata, and Puma, had a developed above-ground mass and low grain productivity. It was statistically determined that the genotype determined the yield of corn grain by 39%, conditions of the year - by 25%, and interaction of factors - by 20%. In experiments with soybean on leached and southern chernozem, the grain yield depended on the genotype by 42%, the conditions of the year - by 27% and the interaction of factors - by 16%, on grey forest medium loamy soil, respectively, 47; 30 and 22%.

Presents research results (2019-2022) to assess the effectiveness of grain crop yield programming: soft spring wheat, spring barley, and oats using geoinformation technologies in satellite navigation applications. Experiments were conducted in the forest-steppe of the Novosibirsk Priob’ye region on leached chernozem in JSC livestock breeding farm “Irmen.”, Ordynsky district, Novosibirsk region. The authors programmed yields on the main grain crops: spring soft wheat varieties Novosibirskaya 31, spring barley Margret and oats varieties Rovesnik and Max. The use of balanced nutrition on the background of their differentiated application in combination with geoinformation technologies and satellite navigation helped to increase the parameters of average leaf area and FSP of soft spring wheat by 24%, spring barley by 22%, and oats by 27%.Fertilizer application on the programmed yield of 4.5-6 t / ha is efficient on the background of differentiated use of mineral fertilizers with justifiability yield programming 117%. The authors proved the dependence of grain crop yield on fertilizers by 38% and weather conditions - by 26% using a statistical method. Balanced doses of mineral nutrition at their differential application increased the protein content of cereal crops by 2%, starch by 4, fat by 0.1 and fiber by 0.2%. The use of yield programming has significantly increased its performance with good product quality. The efficiency of this agricultural technique in the system of optimal use of geoinformation technology with satellite navigation and drones averages 96%. This farming technique is cost-effective, and the level of profitability of production of major crops reached 182%.

In the article, the authors presented the results of the experimental work (2019-2021) to study the application of differentiated fertilizer application on cereal crops. Western Siberia is a region with risky conditions for stable grain crop production. Soft spring wheat and spring barley are the primary crops for food and feed. Soft spring wheat and spring barley are also characterized by a high demand for the most important environmental factors, which are highly variable. Current trends in precision farming imply the need for different technological operations. These operations are differentiated both in time and by individual crop rotation fields. At the same time, operations take more fully into account the bio-resource potential of cultivated agricultural enterprises. And these operations also take into account soil and terrain features, which are controlled by the aggregates and their links to technological operations. In the field, the authors used aggregates with differentiated application rates of fertilizers or crop protection products. The yield mapping is an element of precision farming technology. This technology is achieved by signal sensors on combined and onboard computers and GPS receivers during the harvesting period. An accurate map of the field, showing the characteristics of each field section, enables rational resource allocation and increases yields from each section of the field. The availability of digital maps ensures the optimization of fertilizer, seed, and water application to each section of the field. Under the conditions of the JSC Livestock Breeding Farm Irmen, Ordynsiky district, Novosibirsk region, the data are entered into the on-board computer of agricultural equipment going into the field, and innovative control of technological processes in crop production is carried out through GPS navigation. The work aims to improve the technology of the application of differentiated fertilizers to grain crops in the precision agriculture system under forest-steppe conditions in the Novosibirsk region Priobye. The research was carried out in 2019-2021 in the soil and climatic zone of drained forest steppe on leached chernozem at JSC Livestock Breeding Farm «Irmen» on spring wheat varieties Novosibirskaya 31, Licamero, as well as the spring barley variety Paustian. The use of differentiated fertilizer application contributes to an increase in grain yield parameters of spring wheat by 36% and of spring barley varieties by 28%. The study showed that differential fertilizer application contributes to the improvement of grain quality parameters of soft spring wheat.

The authors cited the results of comprehensive experiments on the application of innovative environmentally acceptable growth regulators on industrial crops: potatoes and soybeans. The experiments were conducted in 2019-2021 in the soil and climatic zone of the drained forest-steppe in the conditions of chernozem on potato varieties, Lyubava (early) and Tuleevsky (medium maturing), and soybean on grain Zolotistaya (early maturing). These experimental fields belong to the Educational and Experimental Farm “Praktik” of Novosibirsk Region and the Closed Joint-Stock Company Livestock Breeding Farming “Irmen” of Novosibirsk Region. The maximum photosynthetic performance of Novosil and Ecofus applied to potatoes and soybeans is up to 19 % compared to control. The authors of the study treated crops before sowing as well as on the shoots before the beginning of the phase of budding. The yield against the growth regulators used increased significantly by 21-24 % when Novosil and Ecofus were applied during seed treatment and when spraying vegetative plants before the phase of budding. It was statistically established that the potato yield depended on variety by 30 %, on growth regulators by 33, and on conditions of the year by 23 %.

At present, it is of particular importance to further increase the yield and quality of grain crops under experimental conditions in Western Siberia. Of special meaning is finding new effective growth regulators and bio-fertilizers that promote ecological plasticity, resistance to environmental stressors and pests. The work aims to study the influence of the new biofertilizer Huminatrin on growth and development features, yield and quality of grain crops under conditions of increasing crop productivity in the northern forest-steppe of the Novosibirsk Priobye region. Experimental field was on the fields of JSC “Irmen” livestock breeding farm in Ordynsky district of Novosibirsk region. The authors determined that Huminatrin (a mixture of micronutrients and bacteria) provides an increase in leaf surface parameters in two varieties of wheat by 23% and in spring barley by 34%. The authors treated soil with Huminatrin before sowing in a dose of 2 l/t at operating-liquid flow rate of 10 l/t and during the growing season in the phases of tillering and earing in a dose of 1.5 l/ha (300 l/ha). It was shown that both wheat varieties and spring barley variety “Biom” significantly increased the yield on the background of Huminatrin. On average, the yield increased by 22%. When using Huminatrin, the authors observed an increase in the number of productive stems (up to 408 mg/m2), the number of grains in the ear (up to 32 pcs), weight of 1000 grains (up to 40.86 g). There was also an increase in gluten content - up to 34%; gluten deformation index was about 96. In addition, the authors note the reduction of plant infestation by brown rust to 28% against 47% in the control (water). Indicators such as weight of 1000 grains, weight of grains per plant, the number of grains in the ear form the quality of the crop. Application of Huminatrin allows to achieve high yield quality of two varieties: spring soft wheat and spring barley

The authors conducted comprehensive research on evaluating heterosis hybrids of foreign selection of cabbage in the forest-steppe conditions of the Priob’ye region. We studied economically valuable features of promising mid-late cabbage hybrids and features of their cultivation on darkgrey forest soil. The research was conducted for two years. As a result of research, hybrids with maximum yield, resistance to mucus bacteriosis, and resistance to root cracking were identified. These hybrids give a high work of standard products, retain marketable qualities throughout the storage period, and contribute to a stable income due to the long sale period of commercial products. The reference hybrid Kohl’s F₁ is the highest yielding hybrid. The Arrivist F₁ hybrid is closer to the Kohl’s F₁ hybrid, with a yield lower by 4.4 t/ha. The other hybrids showed profits 1.1 (Galvatron F₁) and 1.35 (Ferro F1) times lower than the Kola F₁. The standard output was between 84% and 94%. The Arriwist F₁ and Ferro F₁ hybrids showed the best persistence. Due to the sound industry, the shelflife of these hybrids is five points. Thus, the authors distinguished the hybrids Arrivist F₁ and Kohl’s F₁ in commercial value and marketability. All the experimental hybrids were also distinguished for their cabbage. The hybrids Arriviste F₁ and Ferro F₁ showed high resistance to disease during cultivation and storage. All experimental hybrids are recommended for cultivation on vegetable farms and private households. The Ferro hybrid is the best marketability, palatability and storage period (until February). Arriviste F₁ is the best hybrid in terms of resistance to bacteriosis. The authors recommend growing and storing all the experimental hybrids to create a conveyor belt for cabbage sales during the winter-spring period.

The achievement of high yields is the primary objective of modern agricultural production. Increasing the volume of the farm output is the focus of every farmer’s attention. Maise is a highly productive crop with a wide range of applications. The food, industrial, and agro-technical importance of maise point to the need for continuous improvement of cultivation technologies, increasing yields and grain quality under local climate conditions to obtain the highest economic efficiency. The conditions of Western Siberia are characterised by insufficient moisture supply during the growing season. States in Western Siberia are represented by a pronounced continental character with cold, long winters and short, hot summers. Thus, insufficient moisture is a limiting factor for the yield of most crops, including maise, especially in some critical periods. In the present study, the authors evaluated maise yield and structure with and without irrigation. A positive effect of irrigation on the elements of maise yield (length, width and circumference of the cob, number of grains in the row and on the rim, weight of 1000 grains) was noted. The authors also note a significant increase in hybrid Kubansky 101 and Katerina SV yield. The biochemical composition of grain: dry matter, sugars, fat, starch, protein and dextyrins content was studied. It was found that the amount of dry weight in grain on rainfed fields is higher than on irrigated fields, while all other indicators are higher with irrigation.

Rapid epimerization of cycloadducts from Huisgen 1,4-dipoles has been investigated through the synthesis of new spiropyrido[2,1-b][1,3]oxazine-pyrroles and repetition of syntheses from the literature.

The work aims to study the effectiveness of herbicides application on potato varieties of different ripeness groups in the conditions northern forest-steppe of the Novosibirsk Ob region. The authors obtained experimental data on leached chernozem in Novosibirsk district, Novosibirsk region, in 2017-2019. In their studies, the authors used common elements of potato cultivation tech- nology. The general background for potatoes in autumn fertiliser was applied at a dose of P60K90. Nitrogen fertilisers were used at a rate of 60 kg/ha in spring under pre-sowing cultivation. Herbicides Gezagard, Lazurit, Zenkor, Boxer, and mixtures of individual herbicides were applied before and after sprouting when potato plants were up to 12-14 cm high. Herbicides significantly reduced the infestation of potato varieties Lubava (early) and Tuleevsky (medium-season). The authors showed that the use of herbicides accelerated the processes of growth and development of potato varieties of two groups of ripeness. There was a significant increase in leaf area, photosynthetic potential, and 23-28% plant productivity. On a background of herbicides increase of yield by 30-35% is observed. There was an increase in the marketability of tubers by 10%. Used herbicides provided good quality and preservation of potato production. The authors note that the double application of herbicides Zenkor and Lazurit offers high rates of weed reduction. Thus yield and quality of presentation have high indicators. In tubers of potatoes, the residual quantity of herbicides is not detected. Application of herbicides economically and energetically practical. The level of profitability exceeds control 1,4 times on a background of double spraying by herbicides Zenkor and Lazurit.

Aim. As part of a large-scale business transformation, Gazprom Neft is implementing new approaches to improving economic efficiency. One of such approaches is the launch of the “Renovation” project program, the purpose of which is to achieve maximum profitability of the basic well stock. The article describes the results of the study and formation of a set of measures to improve the economic efficiency of oil production at the fields of Gazpromneft-Khantos. Materials and methods. The key feature of “Renovation” is that the goal setting and the formation of key performance indicators of project teams are made not from the previously achieved result, but from the potential. To do this, a detailed technical and economic analysis of the factors affecting the profitability of oil production is first carried out, and the potential for increasing profitability is determined. After that, hypotheses are worked out to increase the economic efficiency of production and a set of measures is formed to realize the identified potential. The cross-functional teams of the “Renovation” program projects are formed around the core-team that includes specialists in geology, development, production, engineering, energy management, economics and IT. This allows teams to solve non-standard tasks in a short time, in a complex way, without attracting external leverage, which is an absolute competitive advantage. Results. The program of optimization measures developed by the cross-functional team of the Renovation project will provide the potential for the growth of the profitability of Gazpromneft-Khantos in the period from 2021 to 2030. Conclusions. The article presents the developed and implemented cases on reducing operating costs in various areas — geology and reservoir engineering, energy efficiency, oil production technologies, downhole operations and hydraulic fracturing, oilfield chemistry.

The results of comprehensive research in 2017–2020 on the effectiveness of improving the health of new zoned and promising potato varieties of three groups of ripeness in the conditions of leached chernozem of the forest-steppe of the Novosibirsk Ob region are presented. The energy-saving, environmentally friendly technology of accelerated potato seed production was used. It is shown that the recovery from viruses of the planting material provides an increase in the yield of different potato varieties up to 42% relative to the unhealthy background. The high efficiency of accelerated propagation of planting material with the use of modern aeroponic plants in comparison with hydroponic plants, and growing in a greenhouse and open ground, is established. The developed technology makes it possible to increase the energy efficiency coefficient by 40 % and the level of profitability by 32 %.

The aim of the work is to study the effectiveness of the use of trace elements on potato varieties of different ripeness groups in the northern forest-steppe Ob in the Novosibirsk region. Experimental data were obtained on leached black soil of Novosibirsk region in 2017–2019. The generally accepted technology of potato cultivation was used for the experiments. Since autumn, fertilizers at a dose of P90K90 were applied as a general background for potatoes. Nitrogen fertilizers (60 kg / ha) were applied in the spring for pre-sowing cultivation. Foliar dressing with microelements (Cu, B, Zn) was used at the beginning of the budding phase of potato plants: copper – 20, boron – 45, zinc – 50 g of active ingredient per 1 ha with a working fluid consumption of 300 l/ha. Trace elements increased the growth and development rates of potato varieties of three groups of ripeness. In varieties Liubava (early), Svitanok Kievsky (mid-early), Tuleevsky (mid-season), the use of trace elements contributed to an increase in the development indicators of the photosynthetic apparatus (maximum and average leaf area and plant productivity) by an average of 21%, especially against the background of boron and zinc. Foliar dressing of potatoes increased the yield parameters by an average of 24 % relative to the control (water). The increase in the marketability of tubers reached 12 %. A greater effect was achieved against the background of boron use: a significant increase in yield, dry matter, starch, vitamin C. In variants with microelements, the susceptibility of potatoes by rhizoctonysis decreased.

The results of three-year studies (2016–2018) of the global gene pool of maize hybrids on leached chernozem of the forest-steppe of Western Siberia are presented. The features of the realization of the biological potential of grain productivity are studied. It is shown that an increased level of production intensification allows to realize a high biological potential of corn grain productivity of different ecological-geographical profile. Hybrids of the first generation of domestic and foreign gene pool provided high photosynthetic parameters in relation to the conditions of the forest-steppe Priobye. The maximum leaf area of such hybrids as Clifton F1 and Korifey F1 exceeded 40 thousand m 2 / ha. The photosynthetic potential of Clifton F1 hybrid plants reached 3418 thousand m 2 · day / ha. The latter hybrid was distinguished by a high intensity of plant growth and development, which made it possible to form a high biological potential for the productivity of green mass and grain. Under conditions of irrigated agriculture in the forest-steppe of Western Siberia, the maximum yield of corn hybrids was at the level of 8 t / ha. It was established that maize hybrids Talisman (yield 8.4 t / ha), Gitago (8.3 t / ha), Delitop (7 t / ha), Falcon (6.8 t / ha) had a high ecological plasticity under extreme conditions Western Siberia and ensured high productivity with good quality of grain. The highest grain yield of maize Kuban 101 (maximum 6 t / ha) was found at a seeding rate of 6-7 grains per 1 m running length.

1,6-Diarylhexane-1,3,4,6-tetraones reacted with 2-aminophenol and acetone according to previously unknown pathway leading to the formation of 3-aryl-3-hydroxy-1-{5-[(2-hydroxyphenyl)amino][1,1′-biphenyl]-3-yl}prop-2-en-1-ones. Similar three-component reactions with benzylamine or 2-methoxyaniline afforded mainly 2-(4-aroyl-3-hydroxy-5,5-dimethyl-1,5-dihydro-2H-pyrrol-2-ylidene)-1-arylethan-1-ones.

The application of robotic assistance in pelvic surgery has become popular across multiple specialties during the past decades, facilitating minimally invasive surgery. The most remarkable challenges regarding these procedures are the carbon dioxide pneumoperitoneum and steep Trendelenburg position. The combination of two factors affects the patient additionally or synergistically and have important physiological effects on cardiovascular system. All those changes are usually well tolerated in patients with normal cardiac function, but it can be different in elderly patients or even in patients with underlying heart conditions. In order to provide the proper management of patients undergone the robotic surgery, we aim to thoroughly understand these effects and overview the risks and possible related cardiovascular complications. Further, a short introduction on dangerous areas of robot-assisted pelvic surgery will be briefly reviewed.

1,4-Dipolar cycloaddition has emerged as a powerful tool for the synthesis of various cyclic compounds. In the present work, 1

The results of three-year studies (2019-2021) on improving the technology of accelerated seed production of potato varieties using the apical meristem method in the conditions of gray forest soil in the forest-steppe of Western Siberia are presented. Integrated experiments were carried out on the experimental fields of CJSC SHP “Michurinets” of the Novosibirsk region. Novosibirsk region. It is shown that the new zoned potato varieties of West Siberian breeding, in particular, the Zlatka variety, has a high multiplication factor of 8 in the accelerated propagation system. The fraction from 25 to 50 mm dominates in the crop at the level of 81%. The highest seed productivity was found in the variety of Novosibirsk breeders Zlatka 186 pcs/m2, then Gala - 152 pcs/m2, Lux - 136 pcs/m2, Mishka - 128 pcs/m2, which is on average 3.5 times higher in comparison with Yuna standard variety. According to the multiplication factor, the Zlatka variety dominated - 26, then the Gala variety - 20 tubers and Terra - 18 tubers. It is statistically determined that the yield of seed tubers depends on the genotype by 39% and the conditions of the year - 28% with the interaction of factors - 19.7%.

The results of three-year research during 2019-2021 on the study of the effectiveness of precision farming elements in the forest-steppe of Western Siberia are presented. The experiments were carried out on leached chernozem at the Irmen breeding farm, Ordynsky district, Novosibirsk region. Elements of precision farming were tested on grain crops: spring wheat: varieties Likamero, Novosibirskaya 31 and Omskaya 36; spring barley varieties: Paustian and Margret and oats varieties Rovestnik. Navigation and drones of varying degrees of complexity were used, as well as a complex of differentiated fertilization. The aim of the work was to improve the technology of precision farming in the production of grain crops in the forest-steppe of Western Siberia. The effectiveness of the use of precision farming elements has been established. Due to the introduction of differentiated doses of mineral fertilizers, the grain yield of wheat varieties increased by 27%, spring barley 33% and oats - 23%. Against the background of the use of geolocation technologies in combination with differentiated application of mineral fertilizers, the yield level reached 7 t/ha for spring soft wheat of the Licamero variety, 8 t/ha for spring barley Paustian, and 5.6 t/ha for oats. In order to obtain a high yield of grain crops, doses of mineral fertilizers were applied differentially according to elementary fields up to 25 Oct. per 1 hectare and liquid nitrogen fertilizers up to 200 l/ha UAN-32, which ensured not only a yield of up to 7 t/ha, but also high technological quality of grain with a gluten level in spring soft wheat of the Licamero variety - 36%.

The reaction of 30-bromo-3β,28-diacylbetulin with sodium azide afforded 30-azido-3β,28-diacyloxylup-20(29)-enes. The products were subjected to a CuI/TMEDA-catalyzed click reaction with ethynylferrocene to obtain the corresponding ferrocene-betulin conjugates with a 1,2,3-triazole linker.

The article considers the factors influencing the stability of agrocenoses of corn hybrids in the forest-steppe zone of the Krasnoyarsk Territory. The researches were carried out for three years in the zone of sharply continental climate of the Krasnoyarsk forest-steppe on leached low- and medium-thick chernozems. The main factors influencing the stability of agrocenoses of corn hybrids were determined. So the factor of climatic conditions affects the corn hybrids, its influence is no more than 14%. The factor of pre-sowing preparation and the precursor (cereals and fallow) showed not significant effect, no more than 2%, on the formation of agrocenoses of corn hybrids. Thus, the factor genotype of the hybrid was fundamental in determining its significance for agrocenoses. The main biometric factor that determines the productivity of corn hybrids was also identified. Correlation analysis made it possible to reveal the influence of the average plant height on the productivity index of corn, as well as on the ear weight. But at the same time, it was determined that the mass of corn ears decreases with an increase in the average height, in the structure of the crop agrocenoses of corn hybrids.

A series of ferrocenyl-substituted methyl ketones and chalcones were synthesized and converted to unsymmetrical 4,6-disubstituted pyrimidin-2-amines and 2-(1H-pyrrol-1-yl)pyrimidines in which the ferrocene fragment is separated from the pyrimidine ring by π-deficient (pyridine) or π-excessive (thiophene) heterocycle. The forbidden band gap of the obtained compounds was determined on the basis of their UV spectra ( $$E_{\text{g}}^{\text{opt}}$$ = 1.75‒2.36 eV), and their electrochemical properties were studied. The presence of a thiophene ring between the ferrocene and pyrimidine fragments reduces the redox potential of the ferrocene fragment (20‒30 mV), whereas pyridine spacer between the ferrocene and pyrimidine fragment increases the redox potential of the ferrocene fragment by more than 100 mV.

Comprehensive and accurate identification of structural variations (SVs) from next generation sequencing data remains a major challenge. We develop FusorSV, which uses a data mining approach to assess performance and merge callsets from an ensemble of SV-calling algorithms. It includes a fusion model built using analysis of 27 deep-coverage human genomes from the 1000 Genomes Project. We identify 843 novel SV calls that were not reported by the 1000 Genomes Project for these 27 samples. Experimental validation of a subset of these calls yields a validation rate of 86.7%. FusorSV is available at https://github.com/TheJacksonLaboratory/SVE. Electronic supplementary material The online version of this article (10.1186/s13059-018-1404-6) contains supplementary material, which is available to authorized users.

This paper presents the results of the identification procedure certification and subsequent quantitative determination of the active ingredients of two-component injectable medicines (active ingredient and solvent) using Raman spectroscopy. The main objective of the research was to select approaches for estimating the metrological characteristics of the measurement procedure, which include a consideration of the methodological parameters, along with a provision of the metrological traceability of measurement results to SI units. To this end, the GVET 176-1-2010 State Secondary Measurement Standard for units of mass fraction, mass (molar) concentration of components in solid and liquid substances and materials based on volumetric titration was used. The following substances were chosen as research objects for estimating the metrological characteristics of the measurement procedure: ascorbic acid, novocaine and sodium thiosulphate. The authors of the work have demonstrated the measurement-procedure certification results, whose accurate determination was confirmed by the results of interlaboratory comparisons. The obtained results confirmed the accuracy of the identification procedure and subsequent quantitative determination, hence its applicability for the determination of the active ingredients in two-component injectable medicines. In addition, the possibility of developing reference materials based on the medicines under study is indicated. Further development of this study may be directed at the development of an identification procedure and its certification, with subsequent quantitative determination of the active ingredients of injectable medicines having three components as well as those having a more complex composition.

A facile method for the synthesis of meta-substituted arylamines from acyclic precursors was developed. This method is based on three-component cyclo-condensation/aromatization of in situ generated imines of acetone with 1,3-diketones either under conventional heating or under microwave irradiation. The utility of this methodology is illustrated by the possibility of a gram scale synthesis of various anilines from readily available reagents.

ENCODE comprises thousands of functional genomics datasets, and the encyclopedia covers hundreds of cell types, providing a universal annotation for genome interpretation. However, for particular applications, it may be advantageous to use a customized annotation. Here, we develop such a custom annotation by leveraging advanced assays, such as eCLIP, Hi-C, and whole-genome STARR-seq on a number of data-rich ENCODE cell types. A key aspect of this annotation is comprehensive and experimentally derived networks of both transcription factors and RNA-binding proteins (TFs and RBPs). Cancer, a disease of system-wide dysregulation, is an ideal application for such a network-based annotation. Specifically, for cancer-associated cell types, we put regulators into hierarchies and measure their network change (rewiring) during oncogenesis. We also extensively survey TF-RBP crosstalk, highlighting how SUB1, a previously uncharacterized RBP, drives aberrant tumor expression and amplifies the effect of MYC, a well-known oncogenic TF. Furthermore, we show how our annotation allows us to place oncogenic transformations in the context of a broad cell space; here, many normal-to-tumor transitions move towards a stem-like state, while oncogene knockdowns show an opposing trend. Finally, we organize the resource into a coherent workflow to prioritize key elements and variants, in addition to regulators. We showcase the application of this prioritization to somatic burdening, cancer differential expression and GWAS. Targeted validations of the prioritized regulators, elements and variants using siRNA knockdowns, CRISPR-based editing, and luciferase assays demonstrate the value of the ENCODE resource., ENCODE is a resource comprising thousands of functional genomic datasets. Here, the authors present custom annotation within ENCODE for cancer, highlighting a workflow that can help prioritise key elements in oncogenesis.