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The genus Acacia is a large group of woody legumes containing an enormous amount of morphological diversity in leaf shape. This diversity is at least in part the result of an innovation in leaf development where many Acacia species are capable of developing leaves of both bifacial and unifacial morphologies. While not unique in the plant kingdom, unifaciality is most commonly associated with monocots, and its developmental genetic mechanisms have yet to be explored beyond this group. In this study, we identify an accession of Acacia crassicarpa with high regeneration rates and isolate a clone for genome sequencing. We generate a chromosome-level assembly of this readily transformable clone, and using comparative analyses, confirm a whole-genome duplication unique to Caesalpinoid legumes. This resource will be important for future work examining genome evolution in legumes and the unique developmental genetic mechanisms underlying unifacial morphogenesis in Acacia.

BackgroundWhile early autism intervention can significantly improve outcomes, gaps in implementation exist globally. These gaps are clearest in Africa, where forty percent of the world's children will live by 2050. Task-sharing early intervention to non-specialists is a key implementation strategy, given the lack of specialists in Africa. Naturalistic Developmental Behavioral Interventions (NDBI) are a class of early autism intervention that can be delivered by caregivers. As a foundational step to address the early autism intervention gap, we adapted a non-specialist delivered caregiver coaching NDBI for the South African context, and pre-piloted this cascaded task-sharing approach in an existing system of care.ObjectivesFirst, we will test the effectiveness of the caregiver coaching NDBI compared to usual care. Second, we will describe coaching implementation factors within the Western Cape Department of Education in South Africa.MethodsThis is a type 1 effectiveness-implementation hybrid design; assessor-blinded, group randomized controlled trial. Participants include 150 autistic children (18-72 months) and their caregivers who live in Cape Town, South Africa, and those involved in intervention implementation. Early Childhood Development practitioners, employed by the Department of Education, will deliver 12, one hour, coaching sessions to the intervention group. The control group will receive usual care. Distal co-primary outcomes include the Communication Domain Standard Score (Vineland Adaptive Behavior Scales, Third Edition) and the Language and Communication Developmental Quotient (Griffiths Scales of Child Development, Third Edition). Proximal secondary outcome include caregiver strategies measured by the sum of five items from the Joint Engagement Rating Inventory. We will describe key implementation determinants.ResultsParticipant enrolment started in April 2023. Estimated primary completion date is March 2027.ConclusionThe ACACIA trial will determine whether a cascaded task-sharing intervention delivered in an educational setting leads to meaningful improvements in communication abilities of autistic children, and identify implementation barriers and facilitators.Trial registrationNCT05551728 in Clinical Trial Registry (https://clinicaltrials.gov).

This study evaluated the effect of tannin extract from Acacia mearnsii on intravaginal temperature, production and milk composition of Holstein cows in a subtropical environment. The study period was 29 days and 20 multiparous Holstein cows were selected with an average milk production of 50 ± 6 kg/day and 147 ± 83 days in milk. The cows were separated into two treatments: Control (n = 10) did not receive dietary supplementation. Tannin (n = 10) received Condensed Tannin (Tanac S.A., Montenegro, RS, Brazil) at a dose of 0.19% dry matter (40 g/cow/day). Animals were monitored for milk production and composition as well as intravaginal temperature. The environmental temperature and humidity index (THI) were monitored. The mean THI was 72.39 ± 0.69. The mean intravaginal temperature was 39.17 ± 0.14 °C in the Tannin treatment and 39.03 ± 0.14°C in the Control treatment (P > 0.05). The animals in the Tannin treatment exhibited an intravaginal temperature of 39.2 °C at THI 69.38, and the Control treatment exhibited the same temperature at THI 70.16 (receiver operating characteristic (ROC); P < 0.01). Milk production was higher in the Tannin treatment than in the Control treatment (51.38 ± 0.90 kg/day and 49.94 ± 1.05 kg/day, respectively; P < 0.01). The protein content in milk was higher in the Tannin treatment than in the Control treatment (3.20 ± 0.01% and 3.04 ± 0.01%; respectively; P < 0.01). The results of this study suggested that tannin extract from Acacia mearnsii can attenuate heat stress and its negative effects on animal production. [ABSTRACT FROM AUTHOR]

Summary Three different Acacia seeds (A. retinodes, A. provincialis and A. tenuissima) harvested from different locations and in different years were analysed for their variations in proximate and mineral composition. Results showed no one species had the highest content across all proximate and mineral analyses, for example, A. retinodes Harmans 2020 had the highest ash (3.7%), A. retinodes Harmans 2022 had the highest protein content (31.3%), A. tenuissima Hindmarsh 2020 had the highest fat content (18.5%) and A. provincialis Tarrington 2022 had the highest magnesium content (469 mg/100 g). Principle component analysis was carried out to determine the effect of species, harvest locations and years on the chemical composition. A biplot of the first two principal components with a total of 60.5% variation showed clustering based on harvest years. The compositions of the Acacia seeds were determined to be affected by species, harvest location and year differences. However, a complete gene–environment interaction study is needed to validate this. [ABSTRACT FROM AUTHOR]

The increasing frequency and severity of heatwaves will intensify stress on plants. Given regional variation in heatwave exposure and expected differences in thermal tolerance between species it is unlikely that all plant species will be affected equally by climate change. However, little is currently known about variation in the responses of plants to heat stress, or how those responses differ among closely related species adapted to different environments. Here we quantify the response of 17 Acacia species (175 RNA‐seq libraries), from across Australia's diverse biomes, to a multi‐day experimental heatwave treatment to identify variation in transcriptomic and physiological responses to heat stress. Genes with known heat response functions showed consistent responses across Acacia species. Up to 10% of all genes and over 100 gene families showed significant clinal variation in the magnitude of their expression plasticity across species. Specifically, gene families linked to the temperature stress response were overrepresented among significant relationships with home range temperature conditions. Gene expression responses seen on the first day of the heatwave were more frequently associated with home range climates, while expression responses by day four were more commonly related to photosystem II acclimation. Comparative transcriptomics on non‐model species has the potential to provide key information on stress response plasticity, especially when linked with our understanding of model species. Our study indicates that the pressing challenge to identifying potentially vulnerable species to climate change could be benefited by the further exploration of clinal variation in transcriptome plasticity. [ABSTRACT FROM AUTHOR]

The effectiveness of restoration programs may differ in terms of environmental and socioeconomic metrics, depending on the strategies employed and ecological settings. Thus, we synthesized different restoration strategies and discussed the environmental and socioeconomic factors influencing restoration success. In the present systematic review, we examined peer-reviewed articles published between 1990 and 2024 that discussed strategies and factors impacting land-based restoration. Only 227 of 55,229 articles from ScienceDirect, PubMed, and Google Scholar databases met the inclusion criteria. China, Brazil, and India are more active in conducting studies about land restoration compared to other regions, particularly in megadiverse countries in Asia with high degradation rates. There is a strong emphasis on practical and adaptive restoration strategies, including soil and water management (e.g., agroforestry), the use of native plant species, and weed and invasive species management. The prevalence of Acacia, Leucaena, and Eucalyptus species in restoration programs can inform decisions about effective species selection. Here, a holistic understanding of the complex ecological processes is crucial for the development of effective restoration strategies. Although policy frameworks have received less attention in restoration research, their incorporation into restoration projects can help address policy implications for land-based restoration. Overall, successful restoration necessitates a thorough understanding of the optimal strategies and environmental and socioeconomic factors impacting restoration success. Future restoration initiatives can leverage such an understanding to ensure successful implementation. [ABSTRACT FROM AUTHOR]

Acacia (the wattles) is the largest genus of plants in Australia and its species occupy almost every habitat in the country. Hard galls on the branches, phyllodes and flower parts of wattle trees were noticed from the very early days of British colonisation, but their causes were unknown. Some insects were believed to be involved, but they were not the only cause of wattle galls. In 1889, the Italian mycologist Pier Andrea Saccardo described the rust fungus Uromyces tepperianus from the galls on Acacia salicina , and later, the Victorian government vegetable pathologist, Daniel McAlpine transferred the species tepperianus to his new genus Uromycladium which also included six new species. A total of 28 valid species of Uromycladium , most endemic to Australia, are currently described. Several species of Uromycladium were somehow introduced into South Africa and countries in southeast Asia where they cause significant losses in Acacia plantations, while others are used as biocontrol agents for invasive Acacia species. Short biographies of two of the early collectors of rust galls, the South Australian naturalist and later entomologist Johann Gottlieb Otto Tepper and the Victorian plant pathologist Charles Clifton Brittlebank are also presented. The wattles (Acacia species) are an ancient and iconic Australian genus of trees and shrubs which form part of the identity of the nation. Galls were a common feature on wattle trees, initially being attributed to the activity of some insects, but later a genus of rust fungi, Uromycladium , was found to also cause galls. The lives of two of the early collectors of wattle rust galls, Otto Tepper and Charles Brittlebank, are also illuminated in this paper. Photograph by Alastair McTaggart. [ABSTRACT FROM AUTHOR]

The present study examines the impacts of saponin, extracted from the fruits of Acacia Concinna, in its effectiveness as a dispersant and stabilizer during the pipeline conveyance of iron ore particles. The basic fundamental characteristics of iron ore, which comprises a majority of hematite and has a size of (≤75 µm), have been examined and characterized. Iron ore water slurry (IWS) rheological behavior has been investigated as a function of saponin doses, pH, particle concentration, and the shear rate-shear stress correlation. The change in zeta potential with pH is used to compute the isoelectric point of IWS. An appropriate mechanism has been presented to explicate the interaction between saponin and particles of Iron ore. The surface-modified particles exhibit improved dispersion characteristics in the aqueous solution due to the modification of electrostatic force that the surface charge on each particle causes. Investigation indicates that the optimal proportion of saponin had a significant favorable effect on the fluidity and stability of the suspension. The analysis of the economic impact of the dispersant has been conducted through the evaluation of slurry head loss and specific energy consumption (SEC) pertaining to the implementation of the IWS pipeline in its intended commercial application. [ABSTRACT FROM AUTHOR]

Many invasive alien species (IAS) produce secondary metabolites that affect how other plants function (allelopathic compounds) and can drive other species invasion, as proposed by the invasional meltdown hypothesis. Acacia melanoxylon and Eucalyptus globulus are two of such species. In this study, we analyzed the germination response of seven IAS (Acacia dealbata, Acacia mearnsii, Acacia melanoxylon, Acacia longifolia, Eucalyptus globulus, Paraserianthes lophantha, Phytolacca americana) and a native biotest species (Lactuca sativa) to the application of two different aqueous extracts at two different concentrations of donor species A. melanoxylon and E. globulus. Extract compounds were identified by UHPLC-ESI-QTOF-MS. Eucalyptus aqueous extracts significantly reduced germination in three species (A. dealbata, E. globulus, P. americana). The germination of all the species tested was reduced with acacia aqueous extracts. Our results support the postulates of the Biochemical Recognition Hypothesis in that seeds gauge establishment potential based on phytochemical release of other plants. Furthermore, A. melanoxylon and E. globulus lowered their own germination, suggesting that these species exhibit intraspecific biochemical recognition. We also found support for the Novel Weapons Hypothesis in the case of L. sativa as a native species. Our research shows that phytochemicals are a component of plant-plant interactions, including the invasion process. [ABSTRACT FROM AUTHOR]

In a recent paper in Southern Forests , Jaganathan and co-workers indicated that the low constant-temperature drying method (LCTDM) (103°C for 17 h) is satisfactory for determining moisture content (MC) in seeds with physical dormancy (PY). Would this recommendation apply to Acacia , a large genus (1000+ species) with a seed coat so strong that seeds in the soil can maintain high viability for many decades? MC was assessed, using the LCTDM, in six Australian Acacia species by comparing intact seeds with those cut in half. Halved seeds of all species lost moisture rapidly and their mass had largely stabilised after about 2–3 h. Intact seeds had a wide variation in moisture-loss patterns. In some species, the testa was morphologically unchanged after 24 h at 103°C and this was associated with a relatively slow moisture loss. In other species, the testa had completely shattered after 8 h, leading to a rapid moisture loss. If the LCTDM is used to determine MC in Acacia species, it is advisable to employ a technique that substantially disrupts the testa. Moisture content (MC) is an important property of seeds, associated with many aspects of physiological seed quality. Acacias have very hard seed coats, which means that seeds can survive for decades in the soil, but in standard methods of determining MC (e.g. heating in an oven at 103°C for 17 h), the moisture in the seed might not be able to escape. The standard method can work for some Acacia species; however, to ensure accurate results, seeds should be halved before going into the oven. Image by Geoff Burrows. [ABSTRACT FROM AUTHOR]

Acacia dealbata Link, known as Mimosa in Portugal, is an invasive hardwood species with potential for construction use, but research is limited. The available stock of small-diameter juvenile wood logs can help reduce this gap, but tangential cracking at log ends challenges fastener connections. This study evaluated different treatments to control and reduce end-face cracking in small wood logs during air drying, an economical and environmentally friendly procedure. The extreme two-thirds of sixteen Mimosa logs were subjected to two treatments: one with longitudinal kerfs 15 mm deep along the length (two and three kerfs) and the other with a hollow in the center up to half the length (16 mm and 30 mm diameters). Over 219 days of air drying and compared with the central part, kerfing treatments significantly reduced outer-wood tangential cracking (p < 0.001), with the three kerfs also reducing crack numbers (p < 0.05) but increasing significantly cracks near the pith (p < 0.01). The 30 mm central hollow significantly reduced central perforation cracking (p < 0.05). Prospectively, the results suggest that a combined treatment approach involving cross-cuts could help reduce and/or control end cracking, thereby improving the suitability of wild Mimosa logs for construction use. [ABSTRACT FROM AUTHOR]

Surface water resources have played a fundamental role in the development of human societies. Considering that different agricultural and industrial activities are carried out in the Acacias River basin, the main objective of this research was to analyze the influence of land use on the water quality in this area by identifying the main sources that influence river water quality. The methodology consisted of establishing 12 sampling stations with different land uses at three times. The National Sanitation Foundation-Water Quality Index (NSF-WQI) was applied to the obtained water quality evaluation data. The main results showed that the stations associated with urban centers presented a higher concentration in the following variables: fecal coliforms, biochemical oxygen demand (BOD) and phosphates. The principal components analysis revealed a close relation between the parameters of fecal coliforms, phosphates and BOD, and the pollution processes by organic matter, which are probably related to domestic and industrial wastewater discharges, and to detergents in urbanized areas. The parameters with the greatest range of values were total dissolved solids and turbidity. These results coincide with what was observed in the correlation analysis. Finally, nitrates showed higher concentrations at stations 6 and 7, associated with agricultural and industrial influence areas (i.e., oil palm crops in the basin). This study about the Acacias River is, thus, extremely important for the region, and concludes that the river's self-purifying capacity allows improved water quality in the areas where the predominant land use is not associated with human settlements. [ABSTRACT FROM AUTHOR]

Abstract Green products such as plant tints are becoming more and more well-known worldwide due to their superior biological and ayurvedic properties. In this work, colorant from Amba Haldi (Curcuma aromatica) was isolated using microwave (MW), and bio-mordants were added to produce colorfast shades. Response surface methodology was used to develop a central composite design (CCD), which maximizes coloring variables statistically. The findings from 32 series of experiments show that excellent color depth (K/S = 12.595) was established onto MW-treated silk fabric (RS = 4 min) by employing 65 mL of radiated aqueous extract (RE = 4 min) of 5 pH cutting-edge the existence of 1.5 g/100 mL used sodium chloride at 75 °C for 45 min. It was discovered that acacia (keekar) extract (1%), pomegranate extract (2%), and pistachio extract (1.5%) were present before coloring by the use of bio-mordants. On the other hand, upon dyeing, acacia extract (1.5%), pomegranate extract (1.5%), and pistachio extract (2%) have all shown extremely strong colorfast colors. Comparatively, before dyeing, salts of Al3+ (1.5%), Fe2+ (2%), and TA (1.5%) gave good results; after dyeing, salts of Al3+ (1%) and Fe2+ (1.5%) and TA (2%) gave good results. When applied to silk fabric, MW radiation has increased the production of dyes recovered from rhizomes. Additionally, the right amount of chemical and biological mordants have been added, resulting in color fastness ratings ranging from outstanding to good. Therefore, the natural color extracted from Amba Haldi can be a sustainable option for the dyeing of silk fabric in the textile dyeing and finishing industries.

Beyond ecological and health impacts, invasive alien plant species can generate indirect and direct costs, notably through reduced agricultural yields, restoration, and management of the invaded environment. Acacia dealbata and Ailanthus altissima are invasive plant species that cause particularly significant damage to the railway network in the Mediterranean area. The allelopathic properties of Mediterranean plant species could be used as nature‐based solutions to slow down the spread of such invasive plant species along railway borders. In this context, a mesocosm experiment was set‐up: (i) to test the potential allelopathic effects of Cistus ladanifer, Cistus albidus, and Cotinus coggygria leaf aqueous extracts on seed germination and seedling growth of A. dealbata and A. altissima; (ii) to evaluate whether these effects depend on the extract dose; and finally, (iii) to estimate whether these effects are modified by soil amendment. Leaf aqueous extracts of the three native plant species showed negative effects on both seed germination and seedling growth of the two invasive species. Our results show that the presence of allelochemicals induces a delay in seed germination (e.g., A. dealbata germination lasted up to 269% longer in the presence of high‐dose leaf aqueous extracts of C. coggygria), which can lead to a decrease in individual recruitment. They also highlight a decrease in seedling growth (e.g., high‐dose C. coggygria leaf aqueous extracts induced a 26% decrease in A. dealbata radicle growth), which can alter the competitiveness of invasive species for resource access. Our results also highlight that compost addition limits the inhibitory effect of native Mediterranean plants on the germination of invasive alien plants, suggesting that soil organic matter content can counteract allelopathic effects on invasive alien plants. Thus, our findings revealed that the allelopathic potential of certain Mediterranean plant species could be a useful tool to manage invasive plant species. [ABSTRACT FROM AUTHOR]

Oligonychus perseae Hirst (Acari: Tetranychidae) is a major pest of avocado (Persea americana Mill.) crops, but it also causes damage to other crops and ornamental plants. Classifying and evaluating the type of spatial distribution pattern of mites on their host plants under natural conditions is ecologically and biologically important. Furthermore, to develop strategies to control the populations of pest mites in agroecosystems, it is essential to know the type of spatial pattern of distribution (uniform, random, or aggregate) of that mite on its host plant and its habitat. This study aimed to classify and assess the spatial distribution of O. perseae on A. saligna. The indices of Lloyd's mean crowding indices, Morisita, and mean-variance relationship classified the spatial pattern of dispersion of the persea mites as aggregate. Iwao's mean crowding regression and Taylor's power law confirmed these results, which indicated that O. perseae formed aggregate colonies to reproduce, avoid predators, and socialize. This is also the first report of O. perseae on A. saligna in Baja California and may help us developing sampling programs for O. perseae. Resumen Oligonychus perseae Hirst (Acari: Tetranychidae) es una plaga importante del aguacate (Persea americana Mill.), pero también causa daños a otros cultivos y plantas ornamentales. Clasificar y evaluar el tipo de patrón de distribución espacial de los ácaros en sus plantas hospederas bajo condiciones naturales es importante para desarrollar estrategias de control de sus poblaciones. La distribución espacial de O. perseae sobre A. saligna se midió con los índices de hacinamiento medio de Lloyd, Morisita y la relación mediavarianza clasificaron el patrón de dispersión espacial de los ácaros persea como agregado. La regresión de hacinamiento medio de Iwao y la ley de potencia de Taylor confirmaron estos resultados, indicando que O. perseae formó colonias agregadas para reproducirse, evitar depredadores y socializar. Este es también el primer reporte de O. perseae sobre A. saligna en Baja California, y nos puede sirvir para desarrollar programas de muestro para O. perseae. [ABSTRACT FROM AUTHOR]

The present work investigates a comparative study between mechanical and ImhoflotTM cells on a mini-pilot scale and the applicability of one self-aspirated H-16 cell (hybrid ImhoflotTM cell) on an industrial scale on-site. The VM-04 cell (vertical feed to the separator vessel with 400 mm diameter) was fabricated, developed, and examined. The copper flotation experiments were conducted under similar volumetric conditions for both the ImhoflotTM and mechanical flotation cells keeping the rest of the parameters constant. Further, one H-16 cell was positioned at four different stages in the Gökirmak copper flotation circuit of the Acacia (Türkiye) copper beneficiation plant, i.e., at (i) pre-rougher flotation, (ii) rougher concentrate, (iii) cleaner-scavenger tailing, and (iv) first cleaning concentrate aiming at enhancing the flotation circuit capacity through flash flotation in the rougher stage, reducing copper grade in the final tailing, and increasing cleaning throughput, respectively. Comparative copper flotation tests showed that ultimate recoveries using the ImhoflotTM and mechanically agitated conventional cells were 94% and 74%, respectively. The industrial scale test results indicated that locating one pneumatic H-16 cell with the duty of pre-floating (also known as flash flotation) led to the enrichment ratio and recovery of 4.84 and 89%, respectively. Positioning the H-16 cell at the cleaner-scavenger tailings could diminish the copper tailings grade from 0.43% to 0.31%. Further, a relatively greater enrichment ratio and copper recovery were obtained using only one ImhoflotTM cell (1.76 and 64%) in comparison with employing four existing mechanical cells (50 m3, each cell) in series (1.45 and 60%) at the first cleaner stage. [ABSTRACT FROM AUTHOR]

The aim of this research work was to investigate the influence of parameters such as particle size, mass/solvent ratio, temperature and spray drying on the tannin extraction process in order to develop cost-effective methods with better environmental and structural performance. The pods of Acacia nilotica ssp. tomentosa (ANT) were fractionated into three fractions, coarse fraction (C) (>2 mm), medium fraction (M) (1–2 mm), and fine fraction (F) < 1 mµ), and extracted with different water-to-pod ratios (2:1, 4:1 and 6:1) at different temperatures (30, 50 and 70 °C). The best results were scaled up using the three fractions of ANT, its bark and the bark of Acacia seyal var. seyal (ASS). Part of their extract was spray dried. The tannin content and total polyphenolic materials were evaluated using standard methods. Their adhesives were tested for their tensile strength. Tannins of ASS were characterized by 13C NMR and MALDI-TOF. The results revealed that the fine fraction (F) gave the highest percentage of tannins in both small and scaled-up experiments. The results of the tensile strength conformed to the European standard. The 13C NMR spectra of ANT and ASS showed that the bark contained condensed tannins mainly consisting of procyanidins/prodelphinidin of 70%/30% and 60%/40%, respectively. MALDI–TOF spectra confirmed the results obtained by 13C NMR and detailed the presence of flavonoid monomers and oligomers, some of which were linked to short carbohydrate monomers or dimers. [ABSTRACT FROM AUTHOR]

Acacia mearnsii De Wild. (Black wattle) is a forest species with great social and economical importance for the southern region of Brazil. This species has multifunctional characteristics, being cultivated to produce tannin, cellulose and energy. This research identified black wattle genotypes with competence for adventitious rooting for mini-cutting propagation. Single-bud mini-cuttings were treated with IBA at a concentration of 2000 mg L-1. After 30 days of cultivation in a humid chamber, the mini-cuttings were evaluated for percentages of survival, rooting, number of roots, average length of the three largest roots, and the number of mini-cuttings rooted per mini-stump. The identification of the best genotypes was performed with the Selegen REML/BLUP software. Selection between and within black wattle progenies can be performed based on the number of rooted mini-cuttings, making it possible to select genotypes with high competence for adventitious rooting while maintaining genetic variability for new selection cycles to develop clones for vegetative propagation by mini-cutting. [ABSTRACT FROM AUTHOR]

Diverse studies have showed that the pesticides can cause important damages in ecosystem. Therefore, the development of bio pesticides through nanotechnology can increase efficacy and limit the negative impacts in the environmental that traditionally seen through the use of chemical pesticides. Nanoparticles obtained from plants' extracts can be used for effective pest management as a combined formulation of metal and some other organic material present in the plants. In the present study, our evaluated biosynthesis of nanoparticles of copper used two plant extracts (Acacia cornigera and Annona purpurea), and the Taguchi method was adopted for the synthesis optimization of the following variables of biosynthesis: temperature, pH, extract concentration, and reaction times to maximize the insecticidal activity on Tribolium castaneum. Our results showed that the nanoparticles were successfully synthesized using Acacia cornigera and Anona purpurea extract under optimum conditions under Taguchi L 9 orthogonal design, where copper nanoparticles were obtained with a size of 63–153 nm for using A. cornigera extract, 87–193 nm for A. purpurea extract, and a zeta potential of 9.6 mV and −32.7 mV, respectively. The nanoparticles of copper from A. cornigera showed effective insecticidal activity against Tribolium castaneum, and 90% mortality compared to the 76.6% obtained from nanoparticles of copper from A. purpurea. The results suggest that Cu-nanoparticles derived from both plants could be used as a biocontrol agent of Tribolium castaneum, a pest of stored grain with great economic importance. [ABSTRACT FROM AUTHOR]

If we are to reckon Ursula K. Le Guin as the architect of several liminal ecotopian landscapes, what is most noticeable is her pervasive attitude toward the evolutionary ethic of speculative fiction, which clearly encompasses experimentation on several mind-bending ideas, such as the veracity of accessing other-than-human contexts and models of communication, as well as the examination of complex multisensorial modalities backing such phenomena. As a result, it is evident that Le Guin passionately pronounced the ecocultural imperative as the yardstick of her creative sorties, while she explored radical possibilities for networking life and relationships in fictional geographies. On these lines, the three short stories chosen for discussion--Direction of the Road, The Author of the Acacia Seeds, and Texts--are based on the premise of one perceiving and interpreting what otherwise cannot be easily shaped in words. Can a rhizomatic entity claim sentience and chronicle its saga of psycho-ambulatory movement? Can the eusocial ant be deemed an authentic antiquarian scribe on par with a human? Can the ocean's spatial language of ebb and flow be adequately deciphered? These are earth-centered thematic questions raised and answered by Le Guin in the vein of imaginative realism. So too, we observe, the distinctive feature that characterizes the author's narratives is her emphasis on the subject of geo-linguistics and eco-historicism, whereupon every rock, tree, bird, and insect is deemed the hero of one's own story. [ABSTRACT FROM AUTHOR]

Several Acacia species are aggressive invaders outside their native range, often occupying extensive areas. Traditional management approaches have proven to be ineffective and economically unfeasible, especially when dealing with large infestations. Here, we explain a different approach to complement traditional management by using the waste from Acacia management activities. This approach can provide stakeholders with tools to potentially reduce management costs and encourage proactive management actions. It also prioritizes potential applications of Acacia waste biomass for agriculture and forestry as a way of sequestering the carbon released during control actions. We advocate the use of compost/vermicompost, green manure and charcoal produced from Acacia waste, as several studies have shown their effectiveness in improving soil fertility and supporting crop growth. The use of waste and derivatives as bioherbicides or biostimulants is pending validation under field conditions. Although invasive Acacia spp. are banned from commercialization and cultivation, the use of their waste remains permissible. In this respect, we recommend the collection of Acacia waste during the vegetative stage and its subsequent use after being dried or when dead, to prevent further propagation. Moreover, it is crucial to establish a legal framework to mitigate potential risks associated with the handling and disposal of Acacia waste. [ABSTRACT FROM AUTHOR]

Acacia longifolia (Andrews) Willd. is a legume native to southeast mainland Australia and Tasmania and has two described subspecies: A. l. subsp. longifolia and A. l. subsp. sophorae. The species has been introduced around the world and is considered invasive in several Mediterranean-type climate regions, including in South America, South Africa, and southern Europe. Previous studies comparing native and invasive populations of A. longifolia have focused on its reproductive ecology and population genetics, and little information exists on the species' early life development and how abiotic factors influence it. Here, we performed a glasshouse experiment to compare the phenotypic responses of native and invasive (in Portugal) A. longifolia seedlings to different levels of water and nutrient availability. We found that seedlings of both subspecies responded similarly to different water and nutrient availability conditions in terms of biomass accumulation, root length, the number of phyllodes produced, phyllode water content, and root-to-shoot ratio. However, compared to native seedlings, invasive seedlings had limited capacity for stress responses. We found that invasive seedlings had lower drought tolerance than native seedlings, and thus the speed of invasion by A. longifolia into drier parts of Portugal may be hindered. Our results also hint of a possible role of seed "imprinting" in this species' early growth responses, resulting in different resource allocation strategies such as favouring early growth and development over drought resistance in the invaded range. Further studies are required to better understand the species' abiotic stress responses at the intraspecific level and their relation to its invasiveness. [ABSTRACT FROM AUTHOR]

Since Ethiopia relies on biomass resources for about 90% of its energy sources, problems such as deforestation and soil degradation have been intensified more than ever. To mitigate these problems, a possibility of using excess aquatic biomass such as water hyacinth as alternative energy is being investigated. In this study, fuel characteristics of biochar briquettes made from combination of water hyacinth biochars, different binders, and organic matter used in Ethiopia were evaluated. Water hyacinth was dried and pyrolyzed at 400 and 800°C (WHB400 and WHB800, respectively). Water hyacinth biochar was mixed with different binders (molasses and Ethiopian soil) at a ratio of [6:4:0] without organic matter, or at a ratio of [6:3:1] with organic matter ([water hyacinth biochar:binder:organic matter]). The experimental results showed that WHB400 with molasses as binder without organic matter [6:4:0] was the best mixing ratio as biochar briquette with the highest higher heating value and the greatest compressive strength among all other biochar briquettes. Therefore, this study showed water hyacinth biochar could be a great potential as an alternative fuel to conventional acacia charcoal. [ABSTRACT FROM AUTHOR]

N,N-dimethyltryptamine (DMT) is a psychedelic substance produced within various Acacia species; however, the range of species that contain it and the quantity of the compound found within each species is relatively unknown. This work focused on the identification of DMT in 104 plant samples, representing 79 Acacia species, utilizing a reverse phase liquid chromatographic separation prior to mass spectral characterization with a robust Q-TOF-MS method. The findings of this work determined 51 DMT-containing species, 41 of which were previously unknown to contain the psychedelic. [ABSTRACT FROM AUTHOR]

Cistus ladanifer L., Acacia dealbata L., and Aloysia citrodora Paláu were subject to an optimization procedure for two extraction techniques (heat-assisted extraction (HAE) and ultrasound-assisted extraction (UAE)). The extracts were then analyzed by HPLC-DAD-ESI/MS for their phenolic profile (cistus—15 compounds, acacia—21 compounds, and lemon verbena—9 compounds). The response surface methodology was applied, considering four varying factors: ethanol percentage; extraction time; temperature/power; and S/L ratio, generating two responses (the major phenolic compound, or family of compounds, and the extraction yield). For cistus, both techniques optimized the extraction yield of punicalagins, with UAE proving to be the most efficient extraction method (3.22% ethanol, 22 min, 171 W, and 35 g/L). For acacia, HAE maximized the extraction of procyanidin (74% ethanol, 86 min, 24 °C, and 50 g/L), and UAE maximized the content of myricetin (65% ethanol, 8 min, 50 W, and 50 g/L). For lemon verbena, HAE favored the extraction of martynoside (13% ethanol, 96 min, 49 °C and 17 g/L) and forsythiaside UAE (94% ethanol, 25 min, 399 W, and 29 g/L). The optimal conditions for the extraction of compounds with high added value and potential for use in pharmaceuticals and nutraceuticals were defined. [ABSTRACT FROM AUTHOR]

Small streams and their riparian vegetation are closely linked ecosystems. Thus, the invasion of native riparian forests with non‐native species can impact stream ecosystems.We assessed the effects of the invasion of broadleaf deciduous forests by evergreen, nitrogen‐fixing Acacia species on seasonal variation of relevant instream environmental variables, litterfall in the riparian area, aquatic decomposers, and leaf litter decomposition, by comparing three streams flowing through native forests (native streams) and three streams flowing through invaded forests (invaded streams) in central Portugal. Invaded streams flow through forests composed (almost) of monospecific stands of Acacia trees.Litterfall in the riparian area was sampled with fabric traps and sorted into five categories: leaf (including phyllodes), flower, fruit and seed, wood litter, and other materials. Aquatic hyphomycete conidia suspended in water were sampled to assess conidia concentration and community composition. Leaf litter of Quercus robur was enclosed in coarse‐mesh bags and incubated in streams to assess decomposition rates and associated macroinvertebrate density and community composition. Samples from each variable were collected monthly from streams over 1 year.Aquatic hyphomycete conidia concentration was higher in invaded streams in spring/summer when litter inputs, water temperature, and aquatic nutrient concentrations were higher. In contrast, conidia concentration was lower in invaded streams in autumn/winter as they received less native deciduous leaf litter in autumn than native streams. Aquatic hyphomycete community structure changed, and species richness was lower in invaded streams because aquatic nutrient concentrations were higher and leaf litter species richness was lower.Macroinvertebrate and shredder density in decomposing leaf litter did not differ between native and invaded streams, but litter bags may have artificially increased densities by providing high quality food and/or refuges in streams with poor‐quality resources. Nevertheless, macroinvertebrate community structure changed, and family richness was lower in invaded streams.Finally, decomposition rates of Q. robur leaf litter in coarse‐mesh bags were similar between stream types, despite differences in aquatic decomposer communities.Overall, Acacia invasion changed water quality, litterfall seasonality and composition, and aquatic decomposer communities (especially aquatic hyphomycetes). However, Acacia effects on macroinvertebrate density and leaf litter decomposition rates were less pronounced, suggesting that higher trophic levels may be more resilient to invasion than basal levels, or the invasion time/extent in our invaded streams was not strong enough to affect macroinvertebrates and associated processes. Instream invasion effects on aquatic hyphomycete communities were more strongly mediated by changes in litter inputs rather than increases in aquatic nutrient concentrations because they remained oligotrophic in invaded streams. Simplification of riparian and aquatic communities may render them less efficient in coping with additional environmental changes. Acacia effects might be mitigated by the maintenance of a riparian corridor composed of native vegetation. The protection of non‐invaded riparian galleries and restoration of invaded ones could protect and restore stream ecosystems. [ABSTRACT FROM AUTHOR]

Acacia melanoxylon is highly valued for its commercial applications, with the heartwood exhibiting a range of colors from dark to light among its various clones. The underlying mechanisms contributing to this color variation, however, have not been fully elucidated. In an effort to understand the factors that influence the development of dark heartwood, a comparative analysis was conducted on the microstructure, substance composition, differential gene expression, and metabolite profiles in the sapwood (SW), transition zone (TZ), and heartwood (HW) of two distinct clones, SR14 and SR25. A microscopic examination revealed that heartwood color variations are associated with an increased substance content within the ray parenchyma cells. A substance analysis indicated that the levels of starches, sugars, and lignin were more abundant in SP compared to HW, while the concentrations of phenols, flavonoids, and terpenoids were found to be higher in HW than in SP. Notably, the dark heartwood of the SR25 clone exhibited greater quantities of phenols and flavonoids compared to the SR14 clone, suggesting that these compounds are pivotal to the color distinction of the heartwood. An integrated analysis of transcriptome and metabolomics data uncovered a significant accumulation of sinapyl alcohol, sinapoyl aldehyde, hesperetin, 2′, 3, 4, 4′, 6′-peptahydroxychalcone 4′-O-glucoside, homoeriodictyol, and (2S)-liquiritigenin in the heartwood of SR25, which correlates with the up-regulated expression of CCRs (evm.TU.Chr3.1751, evm.TU.Chr4.654_667, evm.TU.Chr4.675, evm.TU.Chr4.699, and evm.TU.Chr4.704), COMTs (evm.TU.Chr13.3082, evm.TU.Chr13.3086, and evm.TU.Chr7.1411), CADs (evm.TU.Chr10.2175, evm.TU.Chr1.3453, and evm.TU.Chr8.1600), and HCTs (evm.TU.Chr4.1122, evm.TU.Chr4.1123, evm.TU.Chr8.1758, and evm.TU.Chr9.2960) in the TZ of A. melanoxylon. Furthermore, a marked differential expression of transcription factors (TFs), including MYBs, AP2/ERFs, bHLHs, bZIPs, C2H2s, and WRKYs, were observed to be closely linked to the phenols and flavonoids metabolites, highlighting the potential role of multiple TFs in regulating the biosynthesis of these metabolites and, consequently, influencing the color variation in the heartwood. This study facilitates molecular breeding for the accumulation of metabolites influencing the heartwood color in A. melanoxylon, and offers new insights into the molecular mechanisms underlying heartwood formation in woody plants. [ABSTRACT FROM AUTHOR]

Purpose: Biological nitrogen (N) fixation (BNF) of understory acacia species presents a potential way for effectively restoring N in forest systems. This study aimed to quantify the impact of acacia species and biochar application rates on BNF and soil mineral N in a suburban native forest of subtropical Australia in the first 4–5 years after prescribed burning. Method: Plant growth values and BNF were measured to assess the impact of biochar rates at 0, 5, and 10 t ha−1 on different acacia species. Soil NH4+-N and NO3−-N along with their N isotope composition (δ15N) were determined to investigate soil–plant interactions in response to acacia species and biochar application. Results: The application of 10 t ha−1 biochar significantly enhanced the growth of acacia species, and concurrently reduced the loss of NO3−-N at soil depths of 0–5 and 5–10 cm. Compared with Acacia disparimma (percentage of N derived from the atmosphere or %Ndfa: 78.2%), A. leiocalyx demonstrated significant higher BNF ability (%Ndfa: 91.3%). Similarly, A. leiocalyx had better growth, in terms of height (269.1 cm versus 179.6 cm), diameter at ground level (2.62 cm versus 1.94 cm), basal area (6.49 cm2 versus 3.43 cm2) and volume (692.2 cm3 versus 258.0 cm3). This was associated with its ability to promote organic matter mineralization, resulting in the accumulation of 15N-depleted NH4+-N. NH4+-N, acting as a substrate, was transformed into NO3−-N through nitrification. From regression analysis, the efficient absorption of NH4+-N by A. leiocalyx significantly mitigated NH4+-N leaching with increasing soil moisture concentration (SMC), resulting in lower δ15N of NH4+-N, which was more negatively related to SMC (R2 = 0.401), compared to that of A. disparimma (R2 = 0.250) at soil depth of 0–5 cm. The production of NO3−-N was reduced, leading to lower NO3−-N concentrations of A. leiocalyx than A. disparimma at soil depth of 0–5 cm (8.06 µg N g−1 versus 9.61 µg N g−1) and that of 5–10 cm (8.24 µg N g−1 versus 9.21 µg N g−1) respectively. Conclusions: As an effective soil amendment, biochar exhibited promise in reducing mineral N loss and stimulating plant growth in long-term applications of exceeding three years. Higher BNF capacity and greater plant growth were observed with A. leiocalyx, compared with those of A. disparimma. The retention and utilisation of mineral N by A. leiocalyx can be considered as strategy to restore forest soils. [ABSTRACT FROM AUTHOR]

Acacia auriculiformis seed oil (ASO) contains linoleic acid (72%) and vernolic acid, which are capable of rendering it as a salient resource for coating applications. In this investigation, ASO a non-conventional source, was first converted to its fatty acid (AFA), followed by aminolysis and esterification to produce an AFA-based polyester amide resin (APEA), using toluene as an azeotropic solvent. Structural confirmation of APEA was performed by FTIR-ATR and 1HNMR spectroscopy. The AFA and APEA resin were characterized and compared with conventional soybean oil-based polyester amide resins (SPEA). The thermal stability of SPEA (270 °C) and APEA (274 °C) resins was determined and compared. Films were produced by curing the resins with melamine–formaldehyde and aliphatic poly-isocyanate and characterized by mechanical, and chemical resistance tests. These tests revealed that APEA has enhanced chemical resistance and scratch hardness than SPEA. The surface morphology of PEA resins was determined by Scanning electron microscopy (SEM) which shows PEA is compatible with these curing agents that contain different true solvents (based on polarity). To study the corrosion resistance properties, a paint primer was formulated with the resins and 5% zinc phosphate. The metal content of the cured primer film was analyzed by Inductively coupled plasma-optical emission (ICP-OE) spectroscopy. All the findings prove that ASO-based PEA exhibited better performance than the soya-based PEA. The APEA can be used as binders in coating formulations to improve the mechanical as well as corrosion resistance properties. [ABSTRACT FROM AUTHOR]

Concerns on the expanding infestation of several Acacia species in the southern Mediterranean European countries have triggered an ever-growing requirement for costly targeted control actions. Valorizing biomass waste produced could help promote and better finance these actions. For that purpose, wood wastes from invasive control actions were tested regarding their pulp and paper potential, aiming to entice cellulose industries to partake in future conservation actions. Wood waste from the five most pervasive Acacia species was studied (Acacia dealbata Link, Acacia longifolia Willd, Acacia mearnsii De Wild, Acacia melanoxylon R.Br, and Acacia saligna Labill) regarding physical and chemical characteristics, and a central composite design was used to optimize alkali charge and reaction temperature on pulping yield and delignification. Bleached kraft pulps were produced with each species' optimized conditions and for an equitable mixture of all species. Optimized pulp yields (52.6%–53.5%) and pulp polymerization degree (2867–3690) of Acacia species were higher than those of Eucalyptus globulus Labill (used as reference). Optimized bleached pulps were refined and fiber, pulp, and handsheet properties determined. Acacia dealbata and A. longifolia presented high specific wood consumption and lower handsheet strength properties, pointing to overall lower pulping potential, while A. melanoxylon and A. mearnsii characteristics were equal to or higher than those of E. globulus. A. saligna pulp and handsheet characteristics appear more suited for tissue paper. The Acacia mixture achieved acceptable characteristics, enabling the indiscriminate use of Acacia wood regardless of the species. As a shortcoming, the Acacia pulps showed the worst optical characteristics, with brightness dropping substantially with beating (64%–76%) when compared to E. globulus (81%). [ABSTRACT FROM AUTHOR]

Acacia melanoxylon is well known as a valuable commercial tree species owing to its high-quality heartwood (HW) products. However, the metabolism and regulatory mechanism of heartwood during wood development remain largely unclear. In this study, both microscopic observation and content determination proved that total amount of starches decreased and phenolics and flavonoids increased gradually from sapwood (SW) to HW. We also obtained the metabolite profiles of 10 metabolites related to phenolics and flavonoids during HW formation by metabolomics. Additionally, we collected a comprehensive overview of genes associated with the biosynthesis of sugars, terpenoids, phenolics, and flavonoids using RNA-seq. A total of ninety-one genes related to HW formation were identified. The transcripts related to plant hormones, programmed cell death (PCD), and dehydration were increased in transition zone (TZ) than in SW. The results of RT-PCR showed that the relative expression level of genes and transcription factors was also high in the TZ, regardless of the horizontal or vertical direction of the trunk. Therefore, the HW formation took place in the TZ for A. melanoxylon from molecular level, and potentially connected to plant hormones, PCD, and cell dehydration. Besides, the increased expression of sugar and terpenoid biosynthesis-related genes in TZ further confirmed the close connection between terpenoid biosynthesis and carbohydrate metabolites of A. melanoxylon. Furthermore, the integrated analysis of metabolism data and RNA-seq data showed the key transcription factors (TFs) regulating flavonoids and phenolics accumulation in HW, including negative correlation TFs (WRKY, MYB) and positive correlation TFs (AP2, bZIP, CBF, PB1, and TCP). And, the genes and metabolites from phenylpropanoid and flavonoid metabolism and biosynthesis were up-regulated and largely accumulated in TZ and HW, respectively. The findings of this research provide a basis for comprehending the buildup of metabolites and the molecular regulatory processes of HW formation in A. melanoxylon. [ABSTRACT FROM AUTHOR]

Metal oxides and carbon are the prime candidates of gas sensor devices. For the inaugural instance, carbonaceous particulate matter was meticulously derived from the branches of Acacia auriculiformis tree indigenous to Sri Lanka to detect vapor and gas. It is worth noting that biomass derived carbon had hitherto remain unexplored in the realm of vapor and gas detection. Carbon films were fabricated via the doctor blade method. The resistivity characteristic of these carbon samples exhibited discernible variations upon exposure to methanol, ethanol, acetone vapors, and ammonia gas. Notably, the gas-sensing properties of sample derived from the central core and the outer shell of Acacia auriculiformis tree branches were independently assessed in these vapors and gas at ambient temperature. Various analytical techniques, including X-ray diffraction (XRD), UVvisible absorption spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM), were judiciously employed to scrutinize the structural attribute, optical band gap, chemical composition and surface morphology of the aforementioned carbon film samples, respectively. XRD analyses unequivocally confirmed the successful formation of carbonaceous films. FTIR spectra conspicuously delineated the presence of oxygen within the carbon films. Importantly, the optical band gap of our carbon films manifested within the range characteristic of organic carbon. Furthermore, the resistance of the carbon films demonstrated a significant reduction upon adsorption of each vapor. It is noteworthy that while gas sensitivities and response times were superior for carbon derived from the central core of the Acacia tree branches, the recovery times were notably expedited for carbon synthesized from the outer shell of these branches. The zenith of gas sensitivity, an impressive 187%, was recorded in response to 1000 ppm of methanol vapor, when employing carbon sourced from the central core of the acacia tree branches. [ABSTRACT FROM AUTHOR]

The adsorption mechanism of S-Metolachlor in an aqueous solution by sawdust biochar derived from Acacia auriculiformis (SAB) was studied. SAB was manufactured at 500 °C for 4 h under oxygen-limited conditions and characterized for SEM, EDS, pHpzc, BET, and FTIR. The adsorption kinetics, isotherm, and diffusion studies of S-Metolachlor and SAB were further explored. Moreover, the effects of the solution pH were examined on the adsorption of S-Metolachlor by SAB. The BET analysis of SAB was achieved at 106.74 m2.g−1 and the solution pH did not significantly influence the S-Metolachlor adsorption. The adsorption data were fitted into a Langmuir isotherm and the PSO model. The film diffusion coefficient Df (4.93 × 10−11 to 8.17 × 10−11 m2.s−1) and the particle diffusion coefficient Dp (1.68 × 10−11 to 2.65 × 10−11 m2.s−1) were determined and the rate-limiting step of S-Metolachlor adsorption and SAB was governed by liquid film diffusion. The S-Metolachlor adsorption process onto SAB was controlled by multiple mechanisms, including pore filling, H-bonding, hydrophobic interaction, and π-π EDA interactions. H-bonding is the main interaction for the adsorption of S-Metolachlor and SAB. Conclusively, the study illustrates that biochar produced from Acacia auriculiformis sawdust possessed effective adsorption properties for S-Metolachlor herbicide. [ABSTRACT FROM AUTHOR]

Wood mechanical properties of Acacia crassicarpa were assessed at the age of ten years old in a progeny test at Cam Lo, Quang Tri Province, central Vietnam. A total of 208 trees from 52 open-pollinated families were selected for study. Height and diameter at breast height were measured for all selected trees and spiral grain was also measured at breast height on the standing trees using a Chalmers device, which measures the grain angle of the surface wood. Basic density and shrinkage were measured on one 12-mm bark-tobark increment cores taken at breast height from each sampled tree. Microfibril angle was measured on fiber prepared from the core samples using polarized light microscopy. Genetic parameters of the wood and growth traits were estimated using individual-tree linear mixed models. High coefficients of additive genetic variation (CVa, 17-34%) and high heritabilities (h2 = 0.61-0.82) for all wood traits indicated that there is potential to improve basic density, wood shrinkage, grain angle and microfibril angle in the species in central Vietnam. The genetic correlation between DBH and shrinkage was positive and thus unfavourable, whereas correlations between DBH and both spiral grain and microfibril angle were negative and favourable. The Chalmers device appeared to be a reliable indirect method for investigating genetic variation in spiral grain in A. crassicarpa. Index selection, based on genetic parameters and economic weighting dependent on breeding objectives is suggested for development of seed orchards to deliver genetic gain for A. crassicarpa in central Vietnam. [ABSTRACT FROM AUTHOR]

Aims: Nitrogen deficiency in eco-engineered technosol from iron (Fe) ore tailings limits the productivity of colonising soil microbes and pioneer plants, which are critical to further development of the technosol. Symbiotic biological N2 fixation may be a strategy to supply N in the moderately alkaline early technosols since native legumes such as Acacia auriculiformis are tolerant of saline and alkaline soil conditions as those in the technosol. It is hypothesized that tolerant native legume A. auriculiformis could form functional nodules to fix N2 when grown in early eco-engineered technosols. Methods: A. auriculiformis growth and root nodulation in the early tailing technosols were investigated using a glasshouse experiment, and plant N2 fixation was evaluated using the 15 N natural abundance isotope method. Key factors influencing root nodulation and N2 fixation have also been evaluated, including water supply and phosphorous nutrition. Results: The results indicated that A. auriculiformis grew well in the tailing technosols and naturally formed nodules with rhizobia. The nodules were functional in N2 fixation, leading to improved plant N nutrition. The nodulation and N2 fixation were severely limited by water deficiency stress. Improved phosphorous supply favoured nodulation and N2 fixation by A. auriculiformis plants under water deficiency stress. Conclusions: These findings suggested that A. auriculiformis could grow in early tailings technosols and fixed N2, and proper water and phosphorous fertilizer management could improve Acacia plant's performance and N2 fixation functions. It is possible to introduce tolerant native legumes such as A. auriculiformis to improve N supply in the early technosols. [ABSTRACT FROM AUTHOR]

This study evaluated the effect of black wattle (Acacia mearnsii) condensed tannin extract in simple and complex diets for weaned piglets on performance, faecal consistency and serum haptoglobin concentrations. Eighty-eight female and castrated male piglets were used, weaned at 28 ± 3 days old, with an initial body weight of 8.02 ± 1.21 kg, housed in same-sex pairs and distributed in four treatments in a 2 x 2 factorial experiment in a randomised complete block design. The experimental treatments were simple diet (SD), simple diet + tannin (SD+T), complex diet (CD) and complex diet + tannin (CD+T). The simple diets contained lower concentrations of dairy ingredients, inclusion of barley and no addition of zinc oxide, palatant, acidifier and yeast. The diets with tannin were supplemented with 1,850 mg kg-1 of black wattle condensed tannin extract. The feed programme consisted of three diets: Pre-starter I (0–7 days), Pre-starter II (8–21 days) and Starter (22–28 days). Feed and water were available ad libitum. Body weight and feed intake were measured weekly to calculate the average daily feed intake, daily weight gain and feed conversion ratio. The faecal consistency score was measured daily to calculate the diarrhoea occurrence percentage. Blood samples for serum haptoglobin concentration were collected at 7 and 14 days after weaning. The addition of condensed tannin had no effect (P > 0.05) on evaluated performance variables for weaned piglets. The diet type also did not affect the piglets’ performance (P > 0.05). The inclusion of black wattle (Acacia mearnsii) condensed tannin does not affect piglet performance, reduces the incidence of diarrhoea in the first week after weaning and decreases the haptoglobin inflammatory response. [ABSTRACT FROM AUTHOR]

Tree holdings provide important socio-economic and environmental benefits, but invasive alien trees can constrain these benefits. However, some invasive alien plants provide both costs and benefits, making management complex due to varied stakeholder perceptions. Using household surveys, the study explored local people's perceptions of Acacia dealbata invasion and clearing in six villages located in upper Tsitsana communal areas, Eastern Cape Province of South Africa. The results suggest that most respondents are aware of A. dealbata invasion but not its invasion status. The respondents valued A. dealbata for its service provision including firewood, construction poles and shade but also cited negative impacts such as loss of grazing land and water. The respondents were aware of the Working for Water clearing programme and reported employment, income, and are awareness of restored grazing land and increased water availability as key clearing benefits. Although some respondents cleared A. dealbata in their homesteads, a small percentage were opposed to clearing due to the perceived loss of benefits. Overall, the study shows conflicting perceptions on the impacts of A. dealbata invasion and clearing, highlighting the value of considering the social dimensions of invasive alien plant species management to avoid conflict generation. [ABSTRACT FROM AUTHOR]

The world is facing an epidemic of obesity. One of the ways to produce food with reduced or without sugar is with the use of natural sweeteners. Acacia, in addition to maltitol and aspartame as an alternative to sucrose in an instant drink has proven to be an excellent choice for food industry manufacturers. Acacia met expectations not only from a technical point of view in terms of good homogenization but also by providing good solubility in water, as well as good synergy with the other components of the formulation. The good digestibility of dietary fibers only improves the bioavailability of other formulation components in the digestive tract, starting in the oral cavity. In doing so, the protection of the tooth enamel has been fully observed, as well as the maintenance of the low glycemic index. This research aims to make sugar-free instant drinks using different sweeteners. For this purpose, the prepared instant drinks were made with acacia gum at 50%, maltitol at 37.5%, and aspartame at 1%, approximately, as a substitute for sucrose (table sugar). To determine the caloric value of instant drinks, a BeBis software program for food products has been used. For conducting sensory evaluation - the Hedonic Scale method was used, which allows participants to rate their overall liking of the product based on different sensory attributes. Saturated fatty acids and sugars were not detected and had a 0% caloric value, apart from proteins which were present in 0.4% and fat 0.1%, leading to an expected caloric value of approximately 80 kcal on 100 g instant drink with acacia gum. The use of acacia gum, maltitol, and aspartame provides an opportunity to obtain an instant drink that has health aspects, not only as a zero-sugar drink, but also as dietetic fiber, prebiotic, digestive tolerance, glycemic regulation, and enamel protection. That makes this instant drink potentially healthier not only for diabetics but also for the general population. [ABSTRACT FROM AUTHOR]

The scarcity of natural resources makes it essential to develop products that meet environmental requirements. This is also true for the water and wastewater treatment business, where even consolidated processes, such as coagulation and flocculation, must be improved, opening opportunities for searching for alternative options to conventional processes. Among the existing options, tannin-based agents (TBAs) have been highlighted in recent years due to their biodegradability and proven efficiency. However, little is known about the impacts of the production process of these agents on an operational/industrial scale. In this study, an examination of the environmental impacts of the full-scale production (more than 500 tons yearly) of a TBA from Acacia spp. (known as black acacia or mimosa) was carried out. To accomplish this, a life cycle assessment (LCA) was developed using openLCA version 2.0.0 to assess a cradle-to-gate system of 1 kg of packed TBA produced. Additionally, a comparison was made between the impacts of the production of TBA and a conventional water treatment agent, aluminum sulfate, to verify the benefits of producing the former. The most relevant impacts resulting from the production of 1 kg of TBA are observed in the following categories: global warming (1.52 kgCO2-eq); terrestrial (7.67 kg1.4-DCB-eq), freshwater (0.06 kg1.4-DCB-eq), and marine (0.08 kg1.4-DCB-eq) ecotoxicities; carcinogenic (0.10 kg1.4-DCB-eq) and non-carcinogenic (1.36 kg1.4-DCB-eq) human toxicities; and water use (0.02 m3). The main contributors to the impacts were the chemicals ammonium chloride and formaldehyde used, the transport of inputs, and the energy used. The aluminum sulfate showed better performance than the TBA for a greater number of categories; however, the normalization of the impacts showed the TBA as a very interesting option. The results obtained here can be used by TBA producers to act on the most impactful categories so that the production process becomes increasingly sustainable. [ABSTRACT FROM AUTHOR]

Allometric equations are often used to estimate plant biomass allocation to different tissue types from easier-to-measure quantities. Biomass allocation, and thus allometric equations, often differs by species and sometimes varies with nutrient availability. We measured biomass components for five nitrogen-fixing tree species (Robinia pseudoacacia, Gliricidia sepium, Casuarina equisetifolia, Acacia koa, Morella faya) and three non-fixing tree species (Betula nigra, Psidium cattleianum, Dodonaea viscosa) grown in field sites in New York and Hawaii for 4-5 years and subjected to four fertilization treatments. We measured total aboveground, foliar, main stem, secondary stem, and twig biomass in all species, and belowground biomass in Robinia pseudoacacia and Betula nigra, along with basal diameter, height, and canopy dimensions. The individuals spanned a wide size range (

Optimal foraging theory predicts that animals maximise energy intake by consuming the most valuable foods available. When resources are limited, they may include lower-quality fallback foods in their diets. As seasonal herbivore diet switching is understudied, we evaluate its extent and effects across three Kenyan reserves each for Critically Endangered eastern black rhino (Diceros bicornis michaeli) and Grevy's zebra (Equus grevyi), and its associations with habitat quality, microbiome variation, and reproductive performance. Black rhino diet breadth increases with vegetation productivity (NDVI), whereas zebra diet breadth peaks at intermediate NDVI. Black rhino diets associated with higher vegetation productivity have less acacia (Fabaceae: Vachellia and Senegalia spp.) and more grass suggesting that acacia are fallback foods, upending conventional assumptions. Larger dietary shifts are associated with longer calving intervals. Grevy's zebra diets in high rainfall areas are consistently grass-dominated, whereas in arid areas they primarily consume legumes during low vegetation productivity periods. Whilst microbiome composition between individuals is affected by the environment, and diet composition in black rhino, seasonal dietary shifts do not drive commensurate microbiome shifts. Documenting diet shifts across ecological gradients can increase the effectiveness of conservation by informing habitat suitability models and improving understanding of responses to resource limitation. Using metabarcoding, we found that seasonal dietary shifts were associated with slower breeding and acacia are fallback foods for black rhino. Grass was the highest value food for Grevy's zebra. Seasonal diet shifts did not drive microbiome shifts. [ABSTRACT FROM AUTHOR]

Acacia melanoxylon is a fast-growing macrophanerophyte with strong adaptability whose leaf enables heteromorphic development. Light is one of the essential environmental factors that induces the development of the heteroblastic leaf of A. melanoxylon, but its mechanism is unclear. In this study, the seedlings of A. melanoxylon clones were treated with weak light (shading net with 40% of regular light transmittance) and normal light (control) conditions for 90 d and a follow-up observation. The results show that the seedlings' growth and biomass accumulation were inhibited under weak light. After 60 days of treatment, phyllodes were raised under the control condition while the remaining compound was raised under weak light. The balance of root, stem, and leaf biomass changed to 15:11:74 under weak light, while it was 40:15:45 under control conditions. After comparing the anatomical structures of the compound leaves and phyllode, they were shown to have their own strategies for staying hydrated, while phyllodes were more able to control water loss and adapt to intense light. The compound leaves exhibited elevated levels of K, Cu, Ca, and Mg, increased antioxidant enzyme activity and proline content, and higher concentrations of chlorophyll a, carotenoids, ABA, CTK, and GA. However, they displayed a relatively limited photosynthetic capacity. Phyllodes exhibited higher levels of Fe, cellulose, lignin, IAA content, and high photosynthetic capacity with a higher maximum net photosynthetic rate, light compensation point, dark respiration rate, and water use efficiency. The comparative analysis of compound leaves and phyllodes provides a basis for understanding the diverse survival strategies that heteroblastic plants employ to adapt to environmental changes. [ABSTRACT FROM AUTHOR]

Owing to the potentially harmful adverse effects of current anti-inflammatory drugs, there is a need to identify new alternative substances. Thus, this study aimed to perform a phytochemical analysis of A. polyphylla to identify compounds responsible for its anti-inflammatory activity. Several fractions of the A. polyphylla extract were obtained and evaluated in an ex vivo anti-inflammatory assay using fresh human blood. Among the evaluated fractions, the BH fraction displayed the highest percentage of PGE2 inhibition (74.8%) compared to the reference drugs dexamethasone and indomethacin, demonstrating its excellent potential for anti-inflammatory activity. Astragalin (P1), a known 3-O-glucoside of kaempferol, was isolated from the A. polyphylla extract for the first time. In addition, a new compound (P2) was isolated and identified as the apigenin-3-C-glycosylated flavonoid. Astragalin showed moderate PGE2 activity (48.3%), whereas P2 was not anti-inflammatory. This study contributes to the phytochemical studies of A. polyphylla and confirms its anti-inflammatory potential. [ABSTRACT FROM AUTHOR]

Medicinal plants used in the traditional system are the ideal therapeutic candidates for diabetes mellitus. In the present study, we aimed to identify the therapeutic potential of polyphenol from Acacia jacquemontii (AJ). In vitro, we study the characterization (HPLC) of AJ extracts, antioxidant, antidiabetic, and anti-inflammatory activities.In an in-vivo study, Wistar rats were grouped into normal control (NC), diabetic control (DC), glibenclamide (GL) treated, AJ n-hexane extract (AJHE) at a dose of 250 mg/kg, and AJ methanol extract (AJME) at a dose of 250 mg/kg treated groups. Glibenclamide was used as a reference drug and we evaluated the effect of AJ extracts on glycemic indicators. AJ extracts showed the presence of certain flavonoids and phenolic acids. From our results, a significant quantity of total flavonoid and total phenolic contents were observed. AJ extracts showed promising antioxidant effects (FRAP, OH radical scavenging activity), α-glucosidase (AJME: 53.2%, and AJHE: 59.2%), DPP-IV, LOX, and HYA inhibitory activities. AJ extracts significantly improved the biological markers including plasma insulin, HOMA-β, and serum C-peptide levels as well as normalized carbohydrate metabolizing enzyme levels in alloxanized diabetic rats. Histopathological examination demonstrated the hypoglycemic properties of AJ through the regeneration of β-cell in the pancreas. A. jacquemontii improved insulin signaling via up-regulation of genes i.e. ngn-3, Ins-1, Ins-2, and Pdx-1. The polyphenols in AJ are possibly the major contributors in in-vitro glucosidase, DPP-IV, LOX, and HYA inhibitory activities and maintenance of glucose homeostasis. [ABSTRACT FROM AUTHOR]