1. Phenolic compounds of Eugenia involucrata (Myrtaceae) extracts and associated antioxidant and inhibitory effects on acetylcholinesterase and α-glucosidase
- Author
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Michele Debiasi Alberton, Andreza Cipriani, Adrielli Tenfen, Adrieli Luz de Sousa, André Luís de Gasper, Diogo Alexandre Siebert, Gustavo Amadeu Micke, and Luciano Vitali
- Subjects
Antioxidant ,biology ,Traditional medicine ,010405 organic chemistry ,α glucosidase ,medicine.medical_treatment ,Organic Chemistry ,Myrtaceae ,Plant Science ,biology.organism_classification ,Inhibitory postsynaptic potential ,01 natural sciences ,Biochemistry ,Acetylcholinesterase ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Alpha-glucosidase ,biology.protein ,medicine - Abstract
Eugenia involucrata DC. (Myrtaceae), popularly known as “cereja-do-Rio-Grande”, is a native tree from Brazil, popularly used as a hypoglycemiant. Crude hydroalcoholic extract (CHE) and fractions (insoluble (FI), dichloromethane (FDM), ethyl acetate (FEA) and butanol (FBu)) of leaves were assessed to determine the phenolic chemical composition by HPLC-ESI-MS/MS. 10 compounds were identified, being 7 new for this species: rutin, isoquercitrin, luteolin-7-O-rutinoside, mandelic acid, naringenin, luteolin-7-O-glucoside and salicylic acid. Extract and fractions showed inhibitory activity on acetylcholinesterase (AchE) enzyme (best result: IC50 = 44.19 µg mL−1, for FEA) and α-glucosidase (α-Glu) (best result: IC50 = 31.25 ± 0.15 µg mL−1, for CHE). The observed antioxidant and inhibitory activity on the AchE and α-Glu is due to, at least in part, the presence of phenolic compounds in the samples.
- Published
- 2020
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