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International audience; Methanobrevibacter oralis, an anaerobic methanogenic archaeon, has been previously isolated from the human oral cavity. Here, sequencing a stool isolate (strain JMR01) yielded a 2.065-Mb genome with a 27.78% GC content containing a total of 2,042 open reading frames and 3 clusters of regularly interspaced short palindromic repeat (CRISPR) loci with associated Cas proteins. Address correspondence to Michel Drancourt, michel.drancourt@univ-amu.fr. M ethanobrevibacter oralis was initially isolated from human subgingival plaque (1), and further studies associated this methanogen with subgingival diseases, including periodontitis (2). We recently demonstrated a significant correlation between the M. oralis charge and the severity of periodontitis (3). Furthermore , M. oralis DNA was detected in the human gut, and we previously reported the first isolation of M. oralis (strain JMR01) from the human gut (4). M. oralis strain JMR01 is a strict anaer-obic archaeon with optimal growth at 37°C (pH 7.5) and 1.5 g/liter NaCl. Methane is produced by reducing acetate or H 2-CO 2 as an electron donor. Rumen fluid and vitamin solutions are required for growth and a volatile fatty acid mixture stimulates growth. Phylogenetic classification based on the 16S rRNA gene sequence (GenBank accession number KC616346) confirmed the affiliation of the M. oralis strain JMR01 herein sequenced with the genus Methanobrevibacter and yielded a sequence similarity of 99% with the reference strain M. oralis DSM 7256 (GenBank accession number HE654003). The M. oralis strain JMR01 complete genome was sequenced by combining shotgun and 3-kb paired-end libraries using high-throughput 454 pyrosequencing (454 Life Sciences-Roche, Boulogne-Billancourt, France). Sequence reads were assembled using a Newbler assembler 2.8 (20120726_1306) (Roche), and 136 contigs were generated into 14 scaffolds. A preliminary open reading frame (ORF) prediction was conducted by automated annotation with Glimmer (http://www .cbcb.umd.edu/software/glimmer/) and RAST (5). The annotation was manually curated using BLAST and the nr database of NCBI. The CRISPR finder (http://crispr.u-psud.fr/Server/) was used to detect and identify CRISPR repeat and spacer sequences in the genome. The M. oralis genome consists of a 2,065,764-bp circular chromosome (with a GC content of 27.78%). A total of 2,042 ORFs were recovered, encoding proteins involved in functions previously assigned in other related Methanobrevibacter organisms (6). Likewise, the M. oralis genome contains three CRISPR loci and associated proteins (Cas), as previously found in Methanobre-vibacter arboriphilicus (7). We previously observed that M. oralis, an inhabitant of the oral cavity, survives in the acidity of the stomach (S. Khelaifia and M. Drancourt, unpublished data), giving it an opportunity to establish itself in the digestive tract. Accordingly, we isolated M. oralis from the human gut. The availability of the M. oralis genome will give the opportunity to develop genotyping methods to investigate the diversity of this frequently detected human archaeon. Moreover, the isolation of a new M. oralis strain from the human gut, as well as its genome sequencing, can increase knowledge of this neglected component of the human gut microbiota. Nucleotide sequence accession numbers. The Methanobre-vibacter oralis strain JMR01 genome sequence has been deposited in EMBL under the accession numbers CBWS010000001 through CBWS010000060. The whole-genome shotgun project has been deposited in GenBank under the accession number CBWS000000000. ACKNOWLEDGMENT

Gemmata massiliana is a new Planctomycetes bacterium isolated from a hospital water network in France, using a new culture medium. It is an aerobic microorganism with optimal growth at pH 8, at 30 °C and salinity ≤ 1.25 % NaCl. G. massiliana is resistant to β-lactam antibiotics, due to lack of peptidoglycan in its cell wall.G. massiliana shares a 97 % 16S rRNA gene sequence similarity with the nearest species, Gemmata obscuriglobus; and 99 % similarity with unnamed soil isolates. Its 9,249,437-bp genome consists in one chromosome and no detectable plasmid and has a 64.07 % G + C content, 32.94 % of genes encoding for hypothetical proteins. The genome contains an incomplete 19.6-kb phage sequence, 26 CRISPRs, 3 CAS and 15 clusters of secondary metabolites. G. massiliana genome increases knowledge of a poorly known world of bacteria. Electronic supplementary material The online version of this article (doi:10.1186/s40793-015-0103-0) contains supplementary material, which is available to authorized users.