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Your search keyword '"Agnihotri, Gautam"' showing total 19 results
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19 results on '"Agnihotri, Gautam"'

1. A revised mechanism for the inactivation of bovine liver enoyl-CoA hydratase by (methylenecyclopropyl)formyl-CoA based on unexpected results with the C114A mutant

Author

Agnihotri, Gautam, He, Shouming, Hong, Lin, Dakoji, Srikanth, Withers, Stephen G., and Liu, Hung-wen

Subjects
Biochemistry -- Research, Enzymes -- Physiological aspects, Cobalt -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on the compound (methylenecyclopropyl)formyl-CoA, a bovine liver enoyl-CoA hydratase inactivator. The study of the purified bovine liver enoyl-CoA hydratase mutant has been carried out in determining the role of the residue in the enzyme's catalytic mechanism and the results are reported.

Published
2002

2. Studies on the inactivation of bovine liver enoyl-CoA hydratase by(Methylenecyclopropyl) formyl-CoA: Elucidation of the inactivation mechanism and identification of cysteine-114 as the entrapped nucleophile

Author

Dakoji, Srikanth, Li, Ding, Agnihotri, Gautam, Hui-Qiang, Zhou, and Hung-wen, Liu

Subjects
Glycine -- Chemical properties, Methylene blue -- Chemical properties, Chemistry
Abstract

Inhibitory properties of (methylene cyclopropyl) formyl-CoA (MCPF-CoA), (methylenecyclopropyl) glycine against enoyl-CoA hydratase (ECH) were studied. This helps in selection of inhibitors to control fatty acid metabolism.

Published
2001

3. Novel enzymatic mechanisms in carbohydrate metabolism

Author

He, Xuemei, Agnihotri, Gautam, and Liu, Hung-wen

Subjects
Chemical research -- Analysis, Enzymes -- Physiological aspects, Carbohydrates -- Physiological aspects, Metabolism -- Physiological aspects, Biosynthesis -- Analysis, Chemistry
Abstract

The authors review the publications on the carbohydrates which serve as a link between the sun energy and the metabolic energy. The topic of interest include the enzyme reaction mechanisms involved in the monosaccharide biosynthesis and transformations.

Published
2000

4. The toxicity of methylenecyclopropylglycine: studies of the inhibitory effects of (methylenecyclopropyl)formyl-CoA on enzymes involved in fatty acid metabolism and the molecular basis of its inactivation of enoyl-CoA hydratases

Author

Li, Ding, Agnihotri, Gautam, Dakoji, Srikanth, Oh, Eugene, Lantz, Marily, and Liu, Hung-wen

Subjects
Enzymes -- Structure-activity relationship, Fatty acid metabolism -- Research, Chemistry
Abstract

A detailed study on the effects of (methylenecyclopropyl)formyl-CoA (MCPF-CoA), a toxic metabolite of methylenecyclopropylglycine, on a number of enzymes involved in the metabolism of fatty acids has been conducted. The results confirmed the role of MCPF-CoA as a potent inactivator of enoyl-CoA hydratases (ECHs) despite its minimal effect on the beta-oxidation enzymes used in the study. The function of MCPF-CoA as a rare irreversible inhibitor for ECHs has potential applications in the design of new drugs for controlling fatty acid metabolism.

Published
1999

6. Synergistic Lethality of a Binary Inhibitor of Mycobacterium tuberculosis KasA

Author

Kumar, Pradeep, primary, Capodagli, Glenn C., additional, Awasthi, Divya, additional, Shrestha, Riju, additional, Maharaja, Karishma, additional, Sukheja, Paridhi, additional, Li, Shao-Gang, additional, Inoyama, Daigo, additional, Zimmerman, Matthew, additional, Ho Liang, Hsin Pin, additional, Sarathy, Jansy, additional, Mina, Marizel, additional, Rasic, George, additional, Russo, Riccardo, additional, Perryman, Alexander L., additional, Richmann, Todd, additional, Gupta, Aditi, additional, Singleton, Eric, additional, Verma, Sheetal, additional, Husain, Seema, additional, Soteropoulos, Patricia, additional, Wang, Zhe, additional, Morris, Roxanne, additional, Porter, Gene, additional, Agnihotri, Gautam, additional, Salgame, Padmini, additional, Ekins, Sean, additional, Rhee, Kyu Y., additional, Connell, Nancy, additional, Dartois, Véronique, additional, Neiditch, Matthew B., additional, Freundlich, Joel S., additional, and Alland, David, additional

Published
2018
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7. Discovery of a 3-(4-Pyrimidinyl) Indazole (MLi-2), an Orally Available and Selective Leucine-Rich Repeat Kinase 2 (LRRK2) Inhibitor that Reduces Brain Kinase Activity

Author

Scott, Jack D., primary, DeMong, Duane E., additional, Greshock, Thomas J., additional, Basu, Kallol, additional, Dai, Xing, additional, Harris, Joel, additional, Hruza, Alan, additional, Li, Sarah W., additional, Lin, Sue-Ing, additional, Liu, Hong, additional, Macala, Megan K., additional, Hu, Zhiyong, additional, Mei, Hong, additional, Zhang, Honglu, additional, Walsh, Paul, additional, Poirier, Marc, additional, Shi, Zhi-Cai, additional, Xiao, Li, additional, Agnihotri, Gautam, additional, Baptista, Marco A. S., additional, Columbus, John, additional, Fell, Matthew J., additional, Hyde, Lynn A., additional, Kuvelkar, Reshma, additional, Lin, Yinghui, additional, Mirescu, Christian, additional, Morrow, John A., additional, Yin, Zhizhang, additional, Zhang, Xiaoping, additional, Zhou, Xiaoping, additional, Chang, Ronald K., additional, Embrey, Mark W., additional, Sanders, John M., additional, Tiscia, Heather E., additional, Drolet, Robert E., additional, Kern, Jonathan T., additional, Sur, Sylvie M., additional, Renger, John J., additional, Bilodeau, Mark T., additional, Kennedy, Matthew E., additional, Parker, Eric M., additional, Stamford, Andrew W., additional, Nargund, Ravi, additional, McCauley, John A., additional, and Miller, Michael W., additional

Published
2017
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8. Identification of an unusual [2Fe-2S]-binding motif in the CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase from Yersinia pseudotuberculosis: implication for C-3 deoxygenation in the biosynthesis of 3,6-dideoxyhexoses

Author

Agnihotri, Gautam, Yung-nan Liu, Paschal, Beth M., and Hung-Wen Liu

Subjects
Pseudotuberculosis -- Research, Iron compounds -- Chemical properties, Iron compounds -- Research, Biological sciences, Chemistry
Abstract

The results of the implications for assigning the catalytic function and mechanism of other E(sub 1) homologues are investigated. The results of this investigation can be used to assign functions and mechanism for these and new related enzymes.

Published
2004

10. Biosynthesis of mycarose: isolation and characterization of enzymes involved in the C-2 deoxygenation

Author

Chen, Huawei, Agnihotri, Gautam, Guo, Zhihong, Que, Nanette L.S., Chen, Xuemei H., and Liu, Hung-wen

Subjects
Biosynthesis -- Research, Deoxy sugars -- Research, Enzymes -- Research, Chemistry
Abstract

Research was conducted to confirm the mode of C-2 deoxygenation in the biosynthesis of 2,6-dideoxyhexoses. The biochemical characterization of TylX3 and TylC1 clearly showed that nature uses a metal-assisted dehydration/reduction sequence to eliminate a beta-OH from a ketohexose precursor. A similar beta-elimination mechanism has also been found for C-6 deoxygenation in the biosynthesis of 6-deoxyhexoses, in which the expulsion of the 6-OH from a 4-ketohexose intermediate produces a 4-keto-6-deoxyhexose product.

Published
1999

18. The toxicity of methylenecyclopropylglycine: Studies of the inhibitory effects of...

Author

Ding Li and Agnihotri, Gautam

Subjects
*AMINO acids, *ENZYME inhibitors, *ENZYMES, *FATTY acids, *METABOLISM, *REACTIVITY (Chemistry)
Abstract

Studies the inhibitory effects of (methylenecyclopropyl)formyl-CoA on enzymes involved in fatty acid metabolism and the molecular basis for its inactivation. Inactivation of Enoyl-CoA hydratases; Target specificity and mode of action; Effect on beta-activation enzymes; Modes of inhibition; Mechanism involving covalent trapping.

Published
1999
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19. Purification and Biochemical Characterization of theMycobacterium tuberculosisβ-Ketoacyl-acyl Carrier Protein Synthases KasA and KasB*

Author

Schaeffer, Merrill L., Agnihotri, Gautam, Volker, Craig, Kallender, Howard, Brennan, Patrick J., and Lonsdale, John T.

Abstract

Mycolic acids are vital components of theMycobacterium tuberculosiscell wall, and enzymes involved in their formation represent attractive targets for the discovery of novel anti-tuberculosis agents. Biosynthesis of the fatty acyl chains of mycolic acids involves two fatty acid synthetic systems, the multifunctional polypeptide fatty acid synthase I (FASI), which performs de novofatty acid synthesis, and the dissociated FASII system, which consists of monofunctional enzymes, and acyl carrier protein (ACP) and elongates FASI products to long chain mycolic acid precursors. In this study, we present the initial characterization of purified KasA and KasB, two β-ketoacyl-ACP synthase (KAS) enzymes of the M. tuberculosisFASII system. KasA and KasB were expressed in E. coliand purified by affinity chromatography. Both enzymes showed activity typical of bacterial KASs, condensing an acyl-ACP with malonyl-ACP. Consistent with the proposed role of FASII in mycolic acid synthesis, analysis of various acyl-ACP substrates indicated KasA and KasB had higher specificity for long chain acyl-ACPs containing at least 16 carbons. Activity of KasA and KasB increased with use of M. tuberculosisAcpM, suggesting that structural differences between AcpM and E. coliACP may affect their recognition by the enzymes. Both enzymes were sensitive to KAS inhibitors cerulenin and thiolactomycin. These results represent important steps in characterizing KasA and KasB as targets for antimycobacterial drug discovery.

Published
2001
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