1. Loss of the CBX7 gene expression correlates with a highly malignant phenotype in thyroid cancer
- Author
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Pierlorenzo Pallante, 1, 2 Antonella Federico, 2 Maria Teresa Berlingieri, 1 Mimma Bianco, 1 Angelo Ferraro, 2 Floriana Forzati, 1 Antonino Iaccarino, 3 Maria Russo, 3 Giovanna Maria Pierantoni, 1 Vincenza Leone, 2 Silvana Sacchetti, 2 Giancarlo Troncone, 2, 3 Massimo Santoro, Alfredo Fusco1, P., Pallante, A., Federico, Berlingieri, Mt, Bianco, M, Ferraro, A, Forzati, F, Iaccarino, A, Russo, M, Pierantoni, GIOVANNA MARIA, Leone, V, Sacchetti, S, Troncone, Giancarlo, Santoro, Massimo, and Fusco, Alfredo
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,endocrine system ,endocrine system diseases ,carcinomas ,Chromosomes, Human, Pair 22 ,Blotting, Western ,Thyroid Gland ,Loss of Heterozygosity ,Mice, Nude ,Biology ,medicine.disease_cause ,Malignancy ,Adenoviridae ,thyroid ,Colony-Forming Units Assay ,Loss of heterozygosity ,Thyroid carcinoma ,Mice ,oncogene ,Cell Line, Tumor ,Adenocarcinoma, Follicular ,medicine ,Carcinoma ,Animals ,Humans ,RNA, Messenger ,RNA, Neoplasm ,Thyroid Neoplasms ,Thyroid cancer ,Cyclin-Dependent Kinase Inhibitor p16 ,Cell Proliferation ,Polycomb Repressive Complex 1 ,Reverse Transcriptase Polymerase Chain Reaction ,Thyroid ,cbx 7 ,medicine.disease ,Carcinoma, Papillary ,Rats ,Gene Expression Regulation, Neoplastic ,Repressor Proteins ,medicine.anatomical_structure ,Oncology ,CBX7 ,Adenocarcinoma ,Carcinogenesis - Abstract
Using gene expression profiling, we found that the CBX7 gene was drastically down-regulated in six thyroid carcinoma cell lines versus control cells. The aims of this study were to determine whether CBX7 is related to the thyroid cancer phenotype and to try to identify new tools for the diagnosis and prognosis of thyroid cancer. We thus evaluated CBX7 expression in various snap-frozen and paraffin-embedded thyroid carcinoma tissues of different degrees of malignancy by quantitative reverse transcription-PCR and immunohistochemistry, respectively. CBX7 expression progressively decreased with malignancy grade and neoplasia stage. Indeed, it decreased in an increasing percentage of cases going from benign adenomas to papillary (PTC), follicular, and anaplastic (ATC) thyroid carcinomas. This finding coincides with results obtained in rat and mouse models of thyroid carcinogenesis. CBX7 loss of heterozygosity occurred in 36.8% of PTC and in 68.7% of ATC. Restoration of CBX7 expression in thyroid cancer cells reduced growth rate, with a retention in the G1 phase of the cell cycle, suggesting that CBX7 can contribute to the proliferation of the transformed thyroid cells. In conclusion, loss of CBX7 expression correlates with a highly malignant phenotype in thyroid cancer patients. [Cancer Res 2008;68(16):6770–8]
- Published
- 2008