26 results on '"Anders, Meyer"'
Search Results
2. Recovery of Nutrients from Cod Processing Waters
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Jorge Coque, Charlotte Jacobsen, Bita Forghani, Anders Meyer, Greta Jakobsen, Jens J. Sloth, and Ann-Dorit Moltke Sørensen
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protein ,phosphorus ,flocculation ,flocculants ,ultrafiltration ,microfiltration ,Biology (General) ,QH301-705.5 - Abstract
Liquid side-streams from food industries can be processed and used in food applications and contribute to reduce the environmental footprint of industries. The goal of this study was to evaluate the effectiveness and applicability of protein and phosphorus separation processes, namely microfiltration, ultrafiltration and flocculation, using protein-rich process waters with low (LS) and high (HS) salt content from the processing of salted cod (Gadus morhua). The application of different flocculants (chitosan lactate and Levasil RD442) were evaluated at different concentrations and maturation periods (0, 1 or 3 h). The results showed that different flocculation treatments resulted in different recoveries of the nutrients from LS and HS. Proteins in LS could be most efficiently recovered by using Levasil RD442 0.25% and no maturation period (51.4%), while phosphorus was most efficiently recovered when using Levasil RD442 1.23% and a maturation period of 1 h (34.7%). For HS, most of its protein was recovered using Levasil RD442 1.23% and a maturation period of 1 h (51.8%), while phosphorus was recovered the most using Levasil 1.23% and no maturation period (47.1%). The salt contents allowed interactions through intermolecular forces with Levasil RD442. The ultrafiltration method was effective on HS since it recovered higher percentages of nutrients in the retentate phase (57% of the protein and 46% of the phosphorus) compared to LS.
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- 2023
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3. Multi-layer PLGA-pullulan-PLGA electrospun nanofibers for probiotic delivery
- Author
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Ajalloueian, Fatemeh, Guerra, Priscila R., Bahl, Martin Iain, Torp, Anders Meyer, Hwu, En Te, Licht, Tine Rask, and Boisen, Anja
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- 2022
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- View/download PDF
4. Optimizing oral delivery of next generation probiotics
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Torp, Anders Meyer, Bahl, Martin Iain, Boisen, Anja, and Licht, Tine Rask
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- 2022
- Full Text
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5. The broadening spectrum of spindle cell lipoma and related tumors: A review
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Anders Meyer
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Spindle cell lipoma ,Pleomorphic lipoma ,Atypical spindle cell lipomatous tumor ,Atypical pleomorphic lipomatous tumor ,Atypical spindle cell pleomorphic lipomatous tumor ,RB1 ,Pathology ,RB1-214 - Abstract
Spindle cell/pleomorphic lipoma has a wide range of histologic appearances and frequently presents diagnostic difficulties. All but the most common cases can be easily confused for locally aggressive or malignant entities. Recently, a family of related tumors resembling SCPL but with unusual clinical features and an atypical and aggressive histologic appearance has been described under the umbrella term of atypical spindle cell pleomorphic lipomatous tumors (ASCPLT). Proper identification of these tumors is critical to prevent unwarranted aggressive surgical and medical therapy. This review focuses on the clinical and histopathologic features of SCL and ASCPLT as well as the challenging differential diagnoses these tumors present.
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- 2022
- Full Text
- View/download PDF
6. Fibrous hamartoma of the thigh in a neonate
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Elizabeth A. Waldrop, MD, Heather Von Bevern, MD, and Anders Meyer, MD
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Fibrous hamartoma of infancy ,Infantile tumor ,Hamartoma ,Pediatrics ,RJ1-570 ,Surgery ,RD1-811 - Abstract
Fibrous hamartoma of infancy (FHI) is a rare, benign lesion characterized as a tumor of myofibroblastic origin that has characteristic features of triphasic histology. FHI was first described in 1956 by Reye and formally named by Enzinger in 1965 [1-2]. The lesion is defined as a hamartoma due to the histologic presentation of disorganized mesenchymal, fibrous, and adipose tissue with absence of mitotic figures; this combination of derived tissue without evidence of anaplasia is diagnostic for FHI. These lesions typically arise as a single, solitary mass, are most commonly located on the extremities, trunk, sacrum, or scrotum and are typically 0.5 to 9.0 centimeters in size [3-4]. Only roughly 200 cases have been reported in the literature [3]. The majority of cases occur in young children; 91% of cases arise within the first year of life [4]. Males are more often affected in a ratio of 2.4:1 [4]. Roughly 20% of cases have been documented as congenital (3). Treatment is surgical excision, which is often curative; local recurrence is rare and incidence decreased by obtaining negative margins (8). We present a case of congenital FHI identified at birth.
- Published
- 2021
- Full Text
- View/download PDF
7. Fluidic resistance control enables high-throughput establishment of mixed-species biofilms
- Author
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Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder, and Mette Burmølle
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biofilm analysis ,biofilm device ,biofilm formation ,biofilm seeding ,BioFlux ,CLSM ,Biology (General) ,QH301-705.5 - Abstract
Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow.
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- 2019
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8. Calcified Chondroid Mesenchymal Neoplasm
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Michael E. Kallen, Michael Michal, Anders Meyer, David I. Suster, Nicholas J. Olson, Gregory W. Charville, Raul Perret, and John M. Gross
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Surgery ,Anatomy ,Pathology and Forensic Medicine - Published
- 2023
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9. Biphenotypic sinonasal sarcoma with PAX3::MAML3 fusion transforming into high-grade rhabdomyosarcoma: report of an emerging rare phenomenon
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Anders Meyer, Natálie Klubíčková, Elaheh Mosaieby, Petr Grossmann, Antonina Kalmykova, Olena Koshyk, and Michael Michal
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Cell Biology ,General Medicine ,Molecular Biology ,Pathology and Forensic Medicine - Abstract
We report a case of a 67-year-old male patient with a sinonasal tumor that showed areas of classic biphenotypic sinonasal sarcoma (BSNS) which in some sections sharply transitioned into high-grade rhabdomyosarcoma. Immunohistochemically, the conventional BSNS parts showed S100 protein, SMA, PAX7, and focal MyoD1 expression, whereas desmin and myogenin were negative. In contrast, the cells in high-grade areas expressed desmin, MyoD1, myogenin, and PAX7, while being negative for S100 protein and SMA. Using the Archer FusionPlex assay, the classical PAX3::MAML3 gene fusion was detected. FISH for PAX3 and MAML3 confirmed a break of these genes in both components. Despite aggressive therapy, the tumor progression resulted in the patient’s death. The herein presented case, together with 2 previously published cases of BSNS with high-grade transformation, helps to better understand this novel phenomenon. Although the risk for such transformation appears low, it has important clinical and diagnostic implications which are discussed.
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- 2023
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10. Delivery of E. coli Nissle to the mouse gut by mucoadhesive microcontainers does not improve its competitive ability against strains linked to ulcerative colitis
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Bondegaard, Pi Westi, Torp, Anders Meyer, Guerra, Priscila, Kristensen, Katja Ann, Christfort, Juliane Fjelrad, Krogfelt, Karen Angeliki, Nielsen, Line Hagner, Zor, Kinga, Boisen, Anja, Mortensen, Martin Steen, Bahl, Martin Iain, Licht, Tine Rask, Bondegaard, Pi Westi, Torp, Anders Meyer, Guerra, Priscila, Kristensen, Katja Ann, Christfort, Juliane Fjelrad, Krogfelt, Karen Angeliki, Nielsen, Line Hagner, Zor, Kinga, Boisen, Anja, Mortensen, Martin Steen, Bahl, Martin Iain, and Licht, Tine Rask
- Abstract
For patients with ulcerative colitis (UC), administration of the probiotic E. coli Nissle (EcN) holds promise for alleviation of disease symptoms. The mechanisms are unclear, but it has been hypothesised that a capacity of the probiotic to outcompete potentially detrimental UC-associated E. coli strains plays an important role. However, this could previously not be confirmed in a mouse model of competition between EcN and two UC-associated strains, as reported by Petersen et al. 2011. In the present study, we re-evaluated the idea, hypothesising that delivery of EcN by a micro device dosing system (microcontainers), designed for delivery into the intestinal mucus, could support colonisation and confer a competition advantage compared to classical oral dosing. Six groups of mice were pre-colonised with one of two UC-associated E. coli strains followed by oral delivery of EcN, either in capsules containing microcontainers with freeze-dried EcN powder, capsules containing freeze-dried EcN powder, or as a fresh sucrose suspension. Co-colonisation between the probiotic and the disease-associated strains was observed regardless of dosing method, and no competition advantages linked to microcontainer delivery were identified within this setup. Other approaches are thus needed if the competitive capacity of EcN in the gut should be improved.
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- 2023
11. Delivery of E. coli Nissle to the mouse gut by mucoadhesive microcontainers does not improve its competitive ability against strains linked to ulcerative colitis
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Bondegaard, Pi Westi, primary, Torp, Anders Meyer, additional, Guerra, Priscila, additional, Kristensen, Katja Ann, additional, Christfort, Juliane Fjelrad, additional, Krogfelt, Karen Angeliki, additional, Nielsen, Line Hagner, additional, Zor, Kinga, additional, Boisen, Anja, additional, Mortensen, Martin Steen, additional, Bahl, Martin Iain, additional, and Licht, Tine Rask, additional
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- 2023
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12. Gene Fusion Identification Using Anchor-Based Multiplex PCR and Next-Generation Sequencing
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Elizabeth M Azzato, Daniel H. Farkas, Anders Meyer, Jay E. Brock, Brian P. Rubin, Maureen A. Jakubowski, Yu-Wei Cheng, Sean O Keenan, and Michael D. Weindel
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0301 basic medicine ,medicine.diagnostic_test ,High-Throughput Nucleotide Sequencing ,RNA ,General Medicine ,Computational biology ,Biology ,Immunohistochemistry ,DNA sequencing ,Primer extension ,Fusion gene ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Multiplex polymerase chain reaction ,Nucleic acid ,medicine ,Humans ,Gene Fusion ,Primer (molecular biology) ,Multiplex Polymerase Chain Reaction ,In Situ Hybridization, Fluorescence ,Fluorescence in situ hybridization - Abstract
Background Methods for identifying gene fusion events, such as fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and transcriptome analysis, are either single gene approaches or require bioinformatics expertise not generally available in clinical laboratories. We analytically validated a customized next-generation sequencing (NGS) panel targeting fusion events in 34 genes involving soft-tissue sarcomas. Methods Specimens included 87 formalin-fixed paraffin-embedded (FFPE) tissues with known gene fusion status. Isolated total nucleic acid was used to identify fusion events at the RNA level. The potential fusions were targeted by gene-specific primers, followed by primer extension and nested PCR to enrich for fusion candidates with subsequent bioinformatics analysis. Results The study generated results using the following quality metrics for fusion detection: (a) ≥100 ng total nucleic acid, (b) RNA average unique start sites per gene-specific primer control ≥10, (c) quantitative PCR assessing input RNA quality had a crossing point Conclusions The test validation study demonstrated analytical sensitivity of 98.7% and analytical specificity of 90.0%. The NGS-based panel generated highly concordant results compared to alternative testing methods.
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- 2021
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13. Review and update in the diagnosis of peripheral nerve sheath tumors
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Anders Meyer
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,Neurofibroma ,business.industry ,Melanoma ,Cutaneous neurofibroma ,Nerve sheath ,medicine.disease ,Nerve Sheath Neoplasms ,Diagnosis, Differential ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Neurology ,Peripheral Nerve Sheath Tumors ,medicine ,Humans ,Neurology (clinical) ,Polycomb Repressive Complex 2 ,business ,Neurilemmoma ,030217 neurology & neurosurgery - Abstract
Purpose of review Although tumors with nerve sheath differentiation are vast, the main clinically significant problems faced by the pathologist are the separation of malignant peripheral nerve sheath tumors (MPNSTs) from histologic mimics, the diagnosis of neurofibromatous neoplasms with atypical features, and the separation of cutaneous neurofibromatous neoplasms from melanoma. This review briefly discusses a variety of common nerve sheath tumors and summarizes recent advances on these diagnostic fronts. Recent findings Much of recent work has focused on abnormalities in polycomb repressive complex 2, and the ways in which these abnormalities may be exploited in the diagnosis of MPNSTs. Progress has been made in the diagnostic and clinical understanding of atypical neurofibromatous neoplasms and low-grade MPNSTs. A number of reports have explored the diagnostic distinction between cutaneous neurofibroma and melanoma. Summary New discoveries show promise in the diagnosis of peripheral nerve sheath tumors, but challenges - old and new - remain.
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- 2020
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14. Multi-layer PLGA-pullulan-PLGA electrospun nanofibers for probiotic delivery
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Tine Rask Licht, En-Te Hwu, Anders Meyer Torp, Fatemeh Ajalloueian, Martin Iain Bahl, Priscila R. Guerra, and Anja Boisen
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Colonization ,biology ,Electrospinning ,Chemistry ,General Chemical Engineering ,Probiotics ,Pullulan ,General Chemistry ,biology.organism_classification ,law.invention ,chemistry.chemical_compound ,Probiotic ,PLGA ,Lactobacillus rhamnosus ,Viability ,In vivo ,law ,Nanofiber ,Mucoadhesion ,Rat model ,Multilayer nanofibers ,Food Science ,Biomedical engineering - Abstract
Encapsulation of bacteria into a polymer matrix can potentially enhance the delivery of probiotics. In this study, we present a multilayer electrospun construct to facilitate enhanced delivery of probiotics, where the internal layer is loaded with bacterial cells and the external layers sandwich the internal layer for increased protection and potential mucoadhesion properties. In this proof of concept study, Lactobacillus rhamnosus GG (LGG), which is a robust and well-studied probiotic strain, was encapsulated into pullulan nanofibers, with two electrospun PLGA (Poly-lactic-co-glycolic acid) layers covering it. According to our in vitro study, there was a large decrease in the viability of LGG released from the monolayer sample (LGG:pullulan). However, the multilayer construct maintained excellent viability and an acceptable storage potential. Our in vivo competition study showed that LGG, delivered by multilayer construct, were able to survive intestinal transit and were recovered from all segments of the intestine. Not only did the multilayer construct perform as well as non-encapsulated spray-dried cells in terms of viability and establishment of LGG, but it also showed some cases of increased LGG colonization from fibers in jejunum and cecum compared to spray-dried LGG three days after dosage. We believe, the multilayer construct has the potential to enhance the delivery of the strains that require additional protection, and can benefit from mucus embedment with the help of the covering layers. Particularly, the use of electrospun fibers for the protection of next-generation probiotics sensitive to oxygen and/or gastric conditions could have major commercial interest.
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- 2022
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15. Recurrent Valvular Vegetation: Fooled Me Once, But Won't Fool Me Twice
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John, Fritzlen, Jordan, Tichenor, Carolyn, Moore, Anders, Meyer, Emmanuel, Daon, and Albert J, Eid
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Humans ,Female ,Endocarditis, Bacterial ,Legionnaires' Disease ,Middle Aged ,Whipple Disease - Published
- 2021
16. Fibrous hamartoma of the thigh in a neonate
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Anders Meyer, Heather Von Bevern, and Elizabeth A. Waldrop
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RD1-811 ,Hamartoma ,Thigh ,Pediatrics ,RJ1-570 ,Lesion ,03 medical and health sciences ,0302 clinical medicine ,Scrotum ,medicine ,Fibrous hamartoma ,Anaplasia ,business.industry ,Anatomy ,medicine.disease ,Sacrum ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Pediatrics, Perinatology and Child Health ,030211 gastroenterology & hepatology ,Surgery ,Infantile tumor ,medicine.symptom ,business ,Fibrous hamartoma of infancy - Abstract
Fibrous hamartoma of infancy (FHI) is a rare, benign lesion characterized as a tumor of myofibroblastic origin that has characteristic features of triphasic histology. FHI was first described in 1956 by Reye and formally named by Enzinger in 1965 [ [1] , [2] ]. The lesion is defined as a hamartoma due to the histologic presentation of disorganized mesenchymal, fibrous, and adipose tissue with absence of mitotic figures; this combination of derived tissue without evidence of anaplasia is diagnostic for FHI. These lesions typically arise as a single, solitary mass, are most commonly located on the extremities, trunk, sacrum, or scrotum and are typically 0.5 to 9.0 centimeters in size [ [3] , [4] ]. Only roughly 200 cases have been reported in the literature [ 3 ]. The majority of cases occur in young children; 91% of cases arise within the first year of life [ 4 ]. Males are more often affected in a ratio of 2.4:1 [ 4 ]. Roughly 20% of cases have been documented as congenital (3). Treatment is surgical excision, which is often curative; local recurrence is rare and incidence decreased by obtaining negative margins (8). We present a case of congenital FHI identified at birth.
- Published
- 2021
17. Long-term continuous flow mechanical biventricular support: 9 years and counting
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Anders Meyer, Tom N. Hoel, Arnt E. Fiane, Einar Gude, Kaspar Broch, and Gro Sørensen
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Time Factors ,Heart Ventricles ,medicine.medical_treatment ,Giant Cell Arteritis ,0206 medical engineering ,02 engineering and technology ,030204 cardiovascular system & hematology ,Giant cell myocarditis ,Ventricular Function, Left ,03 medical and health sciences ,0302 clinical medicine ,Afterload ,Internal medicine ,medicine ,Humans ,cardiovascular diseases ,Heart Failure ,Continuous flow ,Ventricular afterload ,business.industry ,Middle Aged ,medicine.disease ,020601 biomedical engineering ,Right Ventricular Assist Device ,Myocarditis ,Treatment Outcome ,Ventricular assist device ,Heart failure ,cardiovascular system ,Cardiology ,Female ,Surgery ,Heart-Assist Devices ,Cardiology and Cardiovascular Medicine ,business ,Follow-Up Studies - Abstract
We report 2 continuous flow HeartWareTM left ventricular assist devices successfully used in a patient with advanced heart failure of giant cell myocarditis origin in a biventricular configuration. Despite technical challenges of adapting a left ventricular assist device engineered for systemic pressure to function as a right ventricular assist device, the addition of dynamic banding on the right ventricular assist device outflow graft allowed successful adaptation of afterload. This patient has now been on biventricular configuration support for 9 years, and remains stable to this day.
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- 2019
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18. OUP accepted manuscript
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Carolyn Moore, Emmanuel Daon, Albert J. Eid, Anders Meyer, Jordan Tichenor, and John Fritzlen
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Microbiology (medical) ,Tropheryma whipplei ,Infectious Diseases ,biology ,business.industry ,medicine ,Forestry ,medicine.symptom ,biology.organism_classification ,Vegetation (pathology) ,business - Published
- 2021
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19. Colon-Specific Delivery of Bioactive Agents Using Genipin-Cross-Linked Chitosan Coated Microcontainers
- Author
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Kinga Zor, Anja Boisen, Juliane Fjelrad Christfort, Line Hagner Nielsen, Tine Rask Licht, Priscila R. Guerra, Anders Meyer Torp, and Khorshid Kamguyan
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Lactobacillus rhamnosus GG ,Biochemistry (medical) ,Biomedical Engineering ,General Chemistry ,Pharmacology ,Colon specific ,Biomaterials ,Chitosan ,chemistry.chemical_compound ,chemistry ,Cross linked chitosan ,Oral administration ,Genipin ,chitosan ,microdevices ,enzyme-triggered release ,local delivery ,genipin - Abstract
Oral administration of probiotics is beneficial for restoring the intestinal microbial balance and for the treatment of gastrointestinal (GI) tract-related disorders. In the current era characterized by the development of next-generation probiotic microorganisms, which are typically less robust toward environmental challenges than the classically applied lactic acid producing probiotics, we anticipate a need for delivery of live organisms directly to the site where they need to colonize. Here, we thus present, for the first time, a proof of concept for using micrometer-sized polymeric containers, for the engineered delivery of probiotics, using spray dried Lactobacillus rhamnosus GG (LGG), as a model probiotic microorganism. To achieve colon-specific delivery, microcontainers are loaded with LGG and sealed with an enzyme-sensitive coating. A genipin-cross-linked chitosan coating is developed that (i) is stable at gastric and intestinal pH; (ii) has tunable swelling; and (iii) is degradable by the colon-specific bacterial enzymes. The chitosan-genipin coated microcontainers are evaluated in vitro, ex vivo, as well as in vivo in a rat model. Our results confirm that the genipin-cross-linked chitosan coating enables an effective local delivery in the cecum and colon without any premature release in the small intestine. Our findings suggest that the integration of modified polysaccharides with ingestible microdevices has great potential for controlled and site-specific delivery of live microorganisms.
- Published
- 2021
- Full Text
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20. Colon-Specific Delivery of Bioactive Agents Using Genipin-Cross-Linked Chitosan Coated Microcontainers
- Author
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Kamguyan, Khorshid, Torp, Anders Meyer, Christfort, Juliane Fjelrad, Guerra, Priscila, Licht, Tine Rask, Hagner Nielsen, Line, Zor, Kinga, Boisen, Anja, Kamguyan, Khorshid, Torp, Anders Meyer, Christfort, Juliane Fjelrad, Guerra, Priscila, Licht, Tine Rask, Hagner Nielsen, Line, Zor, Kinga, and Boisen, Anja
- Abstract
Oral administration of probiotics is beneficial for restoring the intestinal microbial balance and for the treatment of gastrointestinal (GI) tract-related disorders. In the current era characterized by the development of next-generation probiotic microorganisms, which are typically less robust toward environmental challenges than the classically applied lactic acid producing probiotics, we anticipate a need for delivery of live organisms directly to the site where they need to colonize. Here, we thus present, for the first time, a proof of concept for using micrometer-sized polymeric containers, for the engineered delivery of probiotics, using spray dried Lactobacillus rhamnosus GG (LGG), as a model probiotic microorganism. To achieve colon-specific delivery, microcontainers are loaded with LGG and sealed with an enzyme-sensitive coating. A genipin-cross-linked chitosan coating is developed that (i) is stable at gastric and intestinal pH; (ii) has tunable swelling; and (iii) is degradable by the colon-specific bacterial enzymes. The chitosan-genipin coated microcontainers are evaluated in vitro, ex vivo, as well as in vivo in a rat model. Our results confirm that the genipin-cross-linked chitosan coating enables an effective local delivery in the cecum and colon without any premature release in the small intestine. Our findings suggest that the integration of modified polysaccharides with ingestible microdevices has great potential for controlled and site-specific delivery of live microorganisms.
- Published
- 2021
21. Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone Cysts
- Author
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Josephine K Dermawan, Youran Zou, Sheila A Shurtleff, Scott E. Kilpatrick, Steven D. Billings, Yu-Wei Cheng, Zheng Jin Tu, Elizabeth M Azzato, John D. Reith, Omar Habeeb, Brian P. Rubin, John R. Goldblum, Daniel H. Farkas, and Anders Meyer
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Soft Tissue Neoplasm ,Adolescent ,TFE3 ,Bone Neoplasms ,Soft Tissue Neoplasms ,Biology ,DNA sequencing ,Germline ,Pathology and Forensic Medicine ,Diagnosis, Differential ,Young Adult ,Predictive Value of Tests ,Multiplex polymerase chain reaction ,medicine ,Biomarkers, Tumor ,Humans ,Multiplex ,Genetic Predisposition to Disease ,Child ,Gene ,Aged ,Aged, 80 and over ,Gene Expression Profiling ,Soft tissue ,High-Throughput Nucleotide Sequencing ,Infant ,Reproducibility of Results ,General Medicine ,Middle Aged ,Medical Laboratory Technology ,Bone Cysts, Aneurysmal ,Child, Preschool ,Female ,Gene Fusion ,Transcriptome ,Multiplex Polymerase Chain Reaction ,Ubiquitin Thiolesterase - Abstract
Context.— Bone and soft tissue tumors are heterogeneous, diagnostically challenging, and often defined by gene fusions. Objective.— To present our experience using a custom 34-gene targeted sequencing fusion panel. Design.— Total nucleic acid extracted from formalin-fixed, paraffin-embedded (FFPE) tumor specimens was subjected to open-ended, nested anchored multiplex polymerase chain reaction and enrichment of 34 gene targets, thus enabling detection of known and novel fusion partners. Results.— During a 12-month period, 147 patients were tested as part of routine clinical care. Tumor percentage ranged from 10% to 100% and turnaround time ranged from 3 to 15 (median, 7.9) days. The most common diagnostic groups were small round blue cell tumors, tumors of uncertain differentiation, fibroblastic/myofibroblastic tumors, and adipocytic tumors. In-frame fusion transcripts were identified in 64 of 142 cases sequenced (45%): in 62 cases, the detection of a disease-defining fusion confirmed the morphologic impression; in 2 cases, a germline TFG-GPR128 polymorphic fusion variant was detected. Several genes in the panel partnered with multiple fusion partners specific for different diagnoses, for example, EWSR1, NR4A3, FUS, NCOA2, and TFE3. Interesting examples are presented to highlight how fusion detection or lack thereof was instrumental in establishing accurate diagnoses. Novel fusion partners were detected for 2 cases of solid aneurysmal bone cysts (PTBP1-USP6, SLC38A2-USP6). Conclusions.— Multiplex detection of fusions in total nucleic acid purified from FFPE specimens facilitates diagnosis of bone and soft tissue tumors. This technology is particularly useful for morphologically challenging entities and in the absence of prior knowledge of fusion partners, and has the potential to discover novel fusion partners.
- Published
- 2020
22. Validation of a next-generation sequencing oncology panel optimized for low input DNA
- Author
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Elizabeth M. Azzato, Anders Meyer, Carmela Paolillo, Robyn T. Sussman, Jason N. Rosenbaum, Robert Daber, David B. Lieberman, Ashkan Bigdeli, Sydney M. Shaffer, Karthik Ganapathy, Jennifer J.D. Morrissette, Midhat S. Farooqi, Shrey Sukhadia, and Daniel DeSloover
- Subjects
0301 basic medicine ,Clinical Oncology ,Cancer Research ,Base pair ,Low input ,High-Throughput Nucleotide Sequencing ,Read depth ,DNA, Neoplasm ,Computational biology ,Biology ,DNA sequencing ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,chemistry ,Limit of Detection ,030220 oncology & carcinogenesis ,Genetics ,Humans ,Solid tumor ,Molecular Biology ,Gene ,DNA - Abstract
One caveat of next-generation sequencing (NGS)-based clinical oncology testing is the high amount of input DNA required. We sought to develop a focused NGS panel that could capture hotspot regions in relevant genes requiring 0.5-10 ng input DNA. The resulting Penn Precision Panel (PPP) targeted 20 genes containing clinically significant variants relevant to many cancers. One hundred twenty-three samples were analyzed, including 83 solid tumor specimens derived from FFPE. Various input quantities of DNA (0.5-10 ng) were amplified with content-specific PCR primer pools, then sequenced on a MiSeq instrument (Illumina, Inc.) via paired-end, 2 × 186 base pair reads to an average read depth of greater than 6500x. Variants were detected using an in-house analysis pipeline. Clinical sensitivity and specificity were assessed using results from our previously validated solid tumor NGS panel; sensitivity of the PPP is 96.75% (387/400 variants) and specificity is 99.9% (8427/8428 base pairs). Variant allele frequencies (VAFs) are highly concordant across both assays (r = 0.98 p 0.0001). The PPP is a robust, clinically validated test optimized for low-yield solid tumor specimens, capturing a high percentage of clinically relevant variants found by larger commercially available NGS panels while using only 0.5-10 ng of input DNA.
- Published
- 2018
- Full Text
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23. Colon-Specific Delivery of Bioactive Agents Using Genipin-Cross-Linked Chitosan Coated Microcontainers
- Author
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Kamguyan, Khorshid, primary, Torp, Anders Meyer, additional, Christfort, Juliane Fjelrad, additional, Guerra, Priscila R., additional, Licht, Tine Rask, additional, Hagner Nielsen, Line, additional, Zor, Kinga, additional, and Boisen, Anja, additional
- Published
- 2020
- Full Text
- View/download PDF
24. Fluidic resistance control enables high-throughput establishment of mixed-species biofilms
- Author
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Anders Meyer Torp, Mette Burmølle, Mads Frederik Hansen, Henriette Lyng Røder, and Jonas Stenløkke Madsen
- Subjects
Motile bacteria ,Microfluidics ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Mixed species ,Biofilm seeding ,Fluidics ,Biofilm formation ,Throughput (business) ,030304 developmental biology ,BioFlux ,0303 health sciences ,Shear stress ,biology ,Chemistry ,Microfluidic device ,Microbiota ,010401 analytical chemistry ,Biofilm ,Biofilm device ,biology.organism_classification ,0104 chemical sciences ,Biofilm analysis ,Mixed species biofilm ,Biofilms ,Pseudomonas aeruginosa ,Microfluidic flow systems ,Seeding ,Stress, Mechanical ,Biological system ,Shear Strength ,CLSM ,Bacteria ,Biotechnology - Abstract
Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow.
- Published
- 2019
- Full Text
- View/download PDF
25. Fluidic resistance control enables high-throughput establishment of mixed-species biofilms
- Author
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Hansen, Mads Frederik, primary, Torp, Anders Meyer, additional, Madsen, Jonas Stenløkke, additional, Røder, Henriette Lyng, additional, and Burmølle, Mette, additional
- Published
- 2019
- Full Text
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26. Genstande fra Medicinsk Museion
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Meyer, Ion, Torp, Anders Meyer, Meyer, Ion, and Torp, Anders Meyer
- Published
- 2015
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