1. Belimumab concentration measurements using a homologous anti-idiotype immunoassay.
- Author
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Loeff FC, Parodis I, Walhelm T, Jönsen A, Nikolopoulos D, Sjöwall C, Bengtsson AA, Kos D, van Leeuwen A, van den Broek B, Dijk L, Jeremiasse J, Blomjous BS, Voskuyl AE, Wolbink GJ, Bultink IEM, and Rispens T
- Subjects
- Humans, Animals, Immunosuppressive Agents blood, Antibodies, Monoclonal, Humanized therapeutic use, Antibodies, Monoclonal, Humanized immunology, Enzyme-Linked Immunosorbent Assay methods, Drug Monitoring methods, Antibodies, Anti-Idiotypic immunology
- Abstract
Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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