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Cynanchum wilfordii (Maxim.) Hemsl. is a traditional medicinal herb belonging to the Asclepiadoideae subfamily, whose dried roots have been used as traditional medicine in Asia. The complete chloroplast genome of C. wilfordii was generated by de novo assembly using the small amount of whole genome sequencing data. The chloroplast genome of C. wilfordii was 161 241 bp long, composed of large single copy region (91 995 bp), small single copy region (19 930 bp) and a pair of inverted repeat regions (24 658 bp). The overall GC contents of the chloroplast genome was 37.8%. A total of 114 genes were annotated, which included 80 protein-coding genes, 30 tRNA genes and 4 rRNA genes. Phylogenetic analysis with the reported chloroplast genomes revealed that C. wilfordii is most closely related to Asclepias nivea (Caribbean milkweed) and Asclepias syriaca (common milkweed) within the Asclepiadoideae subfamily.

Asclepias nivea, spec. nov. 7. Asclepias foliis lanceolatis glabris, caule simplici, umbellis erectis lateralibus solitariis. Asclepias caule erecto simplici annuo, foliis lanceolato-ovatis glabris, pedunculis alternis, umbellis erectis. Gron. virg.27. Apocynum persicariae mitis folio, corniculis lacteis. Dill. elth. 33. t.29. f.32. Apocynum americanum, foliis amygdali longioribus. Plum. spec. 2. Habitat in Virginia & America calidiore. &male, Published as part of Linnaeus, Carolus, 1753, Species plantarum: exhibentes plantas rite cognitas, ad genera relatas, cum differentiis specificis, nominibus trivialibus, synonymis selectis, locis natalibus, secundum systema sexuale digestas, Stockholm :Laurentius Salvius on page 215, DOI: 10.5281/zenodo.3931989