Search

Bacteroidetes are abundant members of the human microbiota, with species occupying the distal gut capable of utilising a myriad of diet- and host-derived glycans. Transport of glycans across the outer membrane (OM) of these bacteria is mediated by SusCD protein complexes, comprising a membrane-embedded barrel and a lipoprotein lid, that are thought to operate via a ‘pedal-bin’ mechanism in which the lids open and close to facilitate substrate binding. However, additional cell surface-exposed lipoproteins, namely surface glycan binding proteins and glycoside hydrolases, play critical roles in the capture and processing of large glycan chains into transport-competent substrates. Despite constituting a crucial mechanism of nutrient acquisition by our colonic microbiota, the interactions between these components in the OM are poorly understood. Here we show that for the levan and dextran utilisation systems of Bacteroides thetaiotaomicron, the additional OM components assemble on the core SusCD transporter, forming stable glycan utilising machines which we term ‘utilisomes’. Single particle electron cryogenic electron microscopy (cryo-EM) structures in the absence and presence of substrate reveal concerted conformational changes that rationalise the role of each component for efficient nutrient capture, as well as providing a direct demonstration of the pedal bin mechanism of substrate capture in the intact utilisome.

Imperfections in analog-to-digital conversion (ADC) cannot be ignored when signal digitization requirements demand both wide dynamic range and high resolution, as is the case for the Majorana Demonstrator 76Ge neutrinoless double-beta decay search. Enabling the experiment’s high-resolution spectral analysis and efficient pulse shape discrimination required careful measurement and correction of ADC nonlinearities. A simple measurement protocol was developed that did not require sophisticated equipment or lengthy data-taking campaigns. A slope-dependent hysteresis was observed and characterized. A correction applied to digitized waveforms prior to signal processing reduced the differential and integral nonlinearities by an order of magnitude, eliminating these as dominant contributions to the systematic energy uncertainty at the double-beta decay $Q$ value.

Cryo-electron microscopy (cryoEM) is a powerful technique for structure determination of macromolecular complexes, via single particle analysis (SPA). The overall process involves i) vitrifying the specimen in a thin film supported on a cryoEM grid; ii) screening the specimen to assess particle distribution and ice quality; iii) if the grid is suitable, collecting a single particle dataset for analysis; and iv) image processing to yield an EM density map. In this protocol, an overview for each of these steps is provided, with a focus on the variables which a user can modify during the workflow and the troubleshooting of common issues. With remote microscope operation becoming standard in many facilities, variations on imaging protocols to assist users in efficient operation and imaging when physical access to the microscope is limited will be described.

In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a “pedal bin” transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the β2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism., In Bacteroidetes, SusCD complexes mediate uptake of large nutrients across the outer membrane. SusCD structures in the apo state and in complex with β2,6 fructo-oligosaccharides reveal several substrate molecules in the binding cavity and suggest details of the pedal bin mechanism employed in glycan import.

In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a “pedal bin” transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the β2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ∼2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism.

The MAJORANA DEMONSTRATOR is an experiment constructed to search for neutrinoless double-beta decays in germanium-76 and to demonstrate the feasibility to deploy a ton-scale experiment in a phased and modular fashion. It consists of two modular arrays of natural and $^{76}\textrm{Ge}$-enriched germanium detectors totaling 44.1 kg (29.7 kg enriched detectors), located at the 4850' level of the Sanford Underground Research Facility in Lead, South Dakota, USA. Data taken with this setup since summer 2015 at different construction stages of the experiment show a clear reduction of the observed background index around the ROI for $0\nu\beta\beta$-decay search due to improvements in shielding. We discuss the statistical approaches to search for a $0\nu\beta\beta$-signal and derive the physics sensitivity for an expected exposure of $10\,\textrm{kg}{\cdot}\textrm{y}$ from enriched detectors using a profile likelihood based hypothesis test in combination with toy Monte Carlo data., Comment: 5 page, 2 figures, to appear in Proceedings of TAUP 2017 - XV International Conference on Topics in Astroparticle and Underground Physics, 24 - 28 July 2017, Sudbury, ON, Canada

Porphyromonas gingivalis, an asaccharolytic member of the Bacteroidetes, is a keystone pathogen in human periodontitis that may also contribute to the development of other chronic inflammatory diseases. P. gingivalis utilizes protease-generated peptides derived from extracellular proteins for growth, but how these peptides enter the cell is not clear. Here, we identify RagAB as the outer-membrane importer for these peptides. X-ray crystal structures show that the transporter forms a dimeric RagA(2)B(2) complex, with the RagB substrate-binding surface-anchored lipoprotein forming a closed lid on the RagA TonB-dependent transporter. Cryo-electron microscopy structures reveal the opening of the RagB lid and thus provide direct evidence for a 'pedal bin' mechanism of nutrient uptake. Together with mutagenesis, peptide-binding studies and RagAB peptidomics, our work identifies RagAB as a dynamic, selective outer-membrane oligopeptide-acquisition machine that is essential for the efficient utilization of proteinaceous nutrients by P. gingivalis.Porphyromonas gingivalis, an oral anaerobe involved in the pathogenesis of periodontitis, relies on extracellular proteases to degrade proteins into peptides for growth, but how these peptides enter the cell is unknown. Here, the authors identify RagAB as the outer-membrane importer for these peptides and solve its structure, elucidating that it works via a 'pedal bin' mechanism of nutrient uptake.

The MAJORANA DEMONSTRATOR is an experiment constructed to search for neutrinoless double-beta decays in germanium-76 and to demonstrate the feasibility to deploy a large-scale experiment in a phased and modular fashion. It consists of two modular arrays of natural and $^{76}$Ge-enriched germanium detectors totalling 44.1 kg, located at the 4850' level of the Sanford Underground Research Facility in Lead, South Dakota, USA. Any neutrinoless double-beta decay search requires a thorough understanding of the background and the signal energy spectra. The various techniques employed to ensure the integrity of the measured spectra are discussed. Data collection is monitored with a thorough set of checks, and subsequent careful analysis is performed to qualify the data for higher level physics analysis. Instrumental background events are tagged for removal, and problematic channels are removed from consideration as necessary., Comment: 5 pages, 2 figures, Proceedings of TAUP 2017 - XV International Conference on Topics in Astroparticle and Underground Physics (Sudbury ON, Canada, July 24-28, 2017)

Neutrinoless double-beta decay searches seek to determine the nature of neutrinos, the existence of a lepton violating process, and the effective Majorana neutrino mass. The {\sc Majorana} Collaboration is assembling an array of high purity Ge detectors to search for neutrinoless double-beta decay in $^{76}$Ge. The {\sc Majorana Demonstrator} is composed of 44.8~kg (29.7 kg enriched in $^{76}$Ge) of Ge detectors in total, split between two modules contained in a low background shield at the Sanford Underground Research Facility in Lead, South Dakota. The initial goals of the {\sc Demonstrator} are to establish the required background and scalability of a Ge-based, next-generation, tonne-scale experiment. Following a commissioning run that began in 2015, the first detector module started physics data production in early 2016. We will discuss initial results of the Module 1 commissioning and first physics run, as well as the status and potential physics reach of the full {\sc Majorana Demonstrator} experiment. The collaboration plans to complete the assembly of the second detector module by mid-2016 to begin full data production with the entire array., Neutrino 2016 proceeding

The MAJORANA Collaboration is constructing the MAJORANA DEMONSTRATOR, an ultralow background, 40-kg modular high purity Ge (HPGe) detector array to search for neutrinoless double-beta decay (0νββ-decay) in 76Ge. The goal of the experiment is to demonstrate a background rate at or below 3 counts/(t-y) in the 4 keV region of interest (ROI) around the 2039 keV Q-value for 76Ge 0νββ-decay. In this paper, the status of the MAJORANA DEMONSTRATOR, including its design and measurements of properties of the HPGe crystals is presented.

The MAJORANA DEMONSTRATOR is searching for neutrinoless double-beta decay in $^{76}$Ge with two modular arrays of natural and $^{76}$Ge-enriched germanium detectors. It is located at the 4850 level of Sanford Underground Research Facility in Lead, South Dakota, USA, and its total mass of germanium detectors is 44.1 kg, of which 29.7 kg is enriched. The analysis of the first 26 kg-yr of data provides an unprecedented energy resolution of 0.13% in the region of interest at 2039 keV and a background level of 15.4 $\pm$ 2.0 counts/(FWHM t yr). It establishes the lower limit of the half-life of neutrinoless double beta decay as 2.7 10$^{25}$ yr in 76Ge at 90% CL. This analysis will be summarized here with an emphasis on the energy determination., MEDEX'19 proceedings. Since it was send to AIP, the last reference was published: S. I. Alvis et al. (Majorana Collaboration) Phys. Rev. C 100, 025501

Author(s): Cuesta, C; Alvis, SI; Arnquist, IJ; Avignone, FT; Barabash, AS; Barton, CJ; Basu, V; Bertrand, FE; Bos, B; Busch, M; Buuck, M; Caldwell, TS; Chan, YD; Christofferson, CD; Chu, PH; Clark, M; Cuesta, C; Detwiler, JA; Efremenko, Y; Ejiri, H; Elliott, SR; Gilliss, T; Giovanetti, GK; Green, MP; Gruszko, J; Guinn, IS; Guiseppe, VE; Haufe, CR; Hegedus, RJ; Hehn, L; Henning, R; Hervas Aguilar, D; Hoppe, EW; Howe, MA; Kidd, MF; Konovalov, SI; Kouzes, RT; Lopez, AM; Martin, RD; Massarczyk, R; Meijer, SJ; Mertens, S; Myslik, J; Othman, G; Pettus, W; Piliounis, A; Poon, AWP; Radford, DC; Rager, J; Reine, AL; Rielage, K; Ruof, NW; Shanks, B; Shirchenko, M; Tedeschi, D; Varner, RL; Vasilyev, S; White, BR; Wilkerson, JF; Wiseman, C; Xu, W; Yakushev, E; Yu, CH; Yumatov, V; Zhitnikov, I; Zhu, BX | Abstract: The Majorana Collaboration is operating an array of high-purity Ge detectors to search for the neutrinoless double-β decay of Ge76. The Majorana Demonstrator consists of 44.1 kg of Ge detectors (29.7 kg enriched to 88% in Ge76) split between two modules constructed from ultraclean materials. Both modules are contained in a low-background shield at the Sanford Underground Research Facility in Lead, South Dakota. We present updated results on the search for neutrinoless double-β decay in Ge76 with 26.0±0.5 kg yr of enriched exposure. With the Demonstrator's energy resolution of 2.53 keV FWHM at Qββ, which is the best among all neutrinoless double-β decay experiments, we observe one event in the region of interest with 0.65 events expected from the estimated background, resulting in a lower limit on the Ge76 neutrinoless double-β decay half-life of 2.7×1025 yr [90% confidence level (CL)] with a median sensitivity of 4.8×1025 yr (90% CL). Depending on the matrix elements used, a 90% CL upper limit on the effective Majorana neutrino mass in the range of 200-433 meV is obtained. The measured background in the configurations with full shielding and optimized grounding is 11.9±2.0 counts/(FWHM t yr).

The MAJORANA DEMONSTRATOR is searching for neutrinoless double-beta decay in 76Ge using arrays of point-contact germanium detectors operating at the Sanford Underground Research Facility. Background results in the neutrinoless double-beta decay region of interest from data taken during construction, commissioning, and the start of full operations have been recently published. A pulse shape analysis cut applied to achieve this result, named AvsE, is described in this paper. This cut is developed to remove events whose waveforms are typical of multi-site energy deposits while retaining (90 +/- 3.5)% of single-site events. This pulse shape discrimination is based on the relationship between the maximum current and energy, and tuned using 228Th calibration source data. The efficiency uncertainty accounts for variation across detectors, energy, and time, as well as for the position distribution difference between calibration and $0\nu\beta\beta$ events, established using simulations., Comment: 8 pages, 8 figures

The MAJORANA Collaboration is constructing the MAJORANA Demonstrator, an ultra-low background, 44-kg modular high-purity Ge (HPGe) detector array to search for neutrinoless double-beta decay in Ge-76. The phenomenon of surface micro-discharge induced by high-voltage has been studied in the context of the MAJORANA Demonstrator. This effect can damage the front-end electronics or mimic detector signals. To ensure the correct performance, every high-voltage cable and feedthrough must be capable of supplying HPGe detector operating voltages as high as 5 kV without exhibiting discharge. R&D measurements were carried out to understand the testing system and determine the optimum design configuration of the high-voltage path, including different improvements of the cable layout and feedthrough flange model selection. Every cable and feedthrough to be used at the MAJORANA Demonstrator was characterized and the micro-discharge effects during the MAJORANA Demonstrator commissioning phase were studied. A stable configuration has been achieved, and the cables and connectors can supply HPGe detector operating voltages without exhibiting discharge., 9 pages, 12 figures, 8 tables, submitted to NIM A

Author(s): Haufe, CR; Reine, AL; Abgrall, N; Alvis, SI; Arnquist, IJ; Avignone, FT; Barabash, AS; Barton, CJ; Bertrand, FE; Bode, T; Bradley, AW; Brudanin, V; Busch, M; Buuck, M; Caldwell, TS; Chan, YD; Christofferson, CD; Chu, PH; Cuesta, C; Detwiler, JA; Dunagan, C; Efremenko, Y; Ejiri, H; Elliott, SR; Gilliss, T; Giovanetti, GK; Green, MP; Gruszko, J; Guinn, IS; Guiseppe, VE; Hehn, L; Henning, R; Hoppe, EW; Howe, MA; Keeter, KJ; Kidd, MF; Konovalov, SI; Kouzes, RT; Lopez, AM; Martin, RD; Massarczyk, R; Meijer, SJ; Mertens, S; Myslik, J; O'Shaughnessy, C; Othman, G; Pettus, W; Poon, AWP; Radford, DC; Rager, J; Rielage, K; Robertson, RGH; Ruof, NW; Shanks, B; Shirchenko, M; Suriano, AM; Tedeschi, D; Trimble, JE; Varner, RL; Vasilyev, S; Vetter, K; Vorren, K; White, BR; Wilkerson, JF; Wiseman, C; Xu, W; Yakushev, E; Yu, CH; Yumatov, V; Zhitnikov, I; Zhu, BX | Abstract: The MAJORANA DEMONSTRATOR is an experiment constructed to search for neutrinoless double-beta decays in germanium-76 and to demonstrate the feasibility to deploy a ton-scale experiment in a phased and modular fashion. It consists of two modular arrays of natural and 76Ge-enriched germanium p-Type point contact detectors totaling 44.1 kg, located at the 4850' level of the Sanford Underground Research Facility in Lead, South Dakota, USA. The DEMONSTRATOR uses custom high voltage cables to bias the detectors, as well as custom signal cables and connectors to read out the charge deposited at each detectors point contact. These low-mass cables and connectors must meet stringent radiopurity requirements while being subjected to thermal and mechanical stress. A number of issues have been identified with the currently installed cables and connectors. An improved set of cables and connectors for the MAJORANA DEMONSTRATOR are being developed with the aim of increasing their overall reliability and connectivity. We will discuss some of the issues encountered with the current cables and connectors as well as our improved designs and their initial performance.

Author(s): Gilliss, T; Abgrall, N; Alvis, SI; Arnquist, IJ; Avignone, FT; Barabash, AS; Barton, CJ; Bertrand, FE; Bode, T; Bradley, AW; Brudanin, V; Busch, M; Buuck, M; Caldwell, TS; Chan, YD; Christofferson, CD; Chu, PH; Cuesta, C; Detwiler, JA; Dunagan, C; Efremenko, Y; Ejiri, H; Elliott, SR; Giovanetti, GK; Green, MP; Gruszko, J; Guinn, IS; Guiseppe, VE; Haufe, CR; Hehn, L; Henning, R; Hoppe, EW; Howe, MA; Keeter, KJ; Kidd, MF; Konovalov, SI; Kouzes, RT; Lopez, AM; Martin, RD; Massarczyk, R; Meijer, SJ; Mertens, S; Myslik, J; O'Shaughnessy, C; Othman, G; Pettus, W; Poon, AWP; Radford, DC; Rager, J; Reine, AL; Rielage, K; Robertson, RGH; Ruof, NW; Shanks, B; Shirchenko, M; Suriano, AM; Tedeschi, D; Trimble, JE; Varner, RL; Vasilyev, S; Vetter, K; Vorren, K; White, BR; Wilkerson, JF; Wiseman, C; Xu, W; Yakushev, E; Yu, CH; Yumatov, V; Zhitnikov, I; Zhu, BX | Abstract: The MAJORANA DEMONSTRATOR is a 76Ge-based neutrinoless double-beta decay (0νββ) experiment. Staged at the 4850 ft level of the Sanford Underground Research Facility, the DEMONSTRATOR operates an array of high-purity p-Type point contact Ge detectors deployed within a graded passive shield and an active muon veto system. The present work concerns the two-neutrino double-beta decay mode (2νββ) of 76Ge. For Ge detectors, having superior energy resolution (0.1%), this mode poses negligible background to the 0νββ mode, even for a ton-scale experiment. However, the measurement of the 2νββ mode allows for careful systematics checks of active detector mass, enrichment fraction, and pulse shape discrimination cuts related to both the 0νββ and 2νββ decay modes. A precision measurement of the 2νββ shape also allows searches for spectral distortions, possibly indicative of new physics, including 0νββχ. Work is underway to construct a full experimental background model enabling a Bayesian fit to the measured energy spectrum and extraction of a precise 2νββ spectrum and half-life.

Author(s): Elliott, SR; Abgrall, N; Arnquist, IJ; Avignone, FT; Barabash, AS; Bertrand, FE; Bradley, AW; Brudanin, V; Busch, M; Buuck, M; Caldwell, TS; Chan, YD; Christofferson, CD; Chu, PH; Cuesta, C; Detwiler, JA; Dunagan, C; Efremenko, Y; Ejiri, H; Fullmer, A; Galindo-Uribarri, A; Gilliss, T; Giovanetti, GK; Green, MP; Gruszko, J; Guinn, IS; Guiseppe, VE; Henning, R; Hoppe, EW; Howe, MA; Jasinski, BR; Keeter, KJ; Kidd, MF; Konovalov, SI; Kouzes, RT; Leon, J; Lopez, AM; Macmullin, J; Martin, RD; Massarczyk, R; Meijer, SJ; Mertens, S; Orrell, JL; O'Shaughnessy, C; Poon, AWP; Radford, DC; Rager, J; Rielage, K; Robertson, RGH; Romero-Romero, E; Shanks, B; Shirchenko, M; Suriano, AM; Tedeschi, D; Trimble, JE; Varner, RL; Vasilyev, S; Vetter, K; Vorren, K; White, BR; Wilkerson, JF; Wiseman, C; Xu, W; Yakushev, E; Yu, CH; Yumatov, V; Zhitnikov, I | Abstract: Neutrinoless double-beta decay searches seek to determine the nature of neutrinos, the existence of a lepton violating process, and the effective Majorana neutrino mass. The Majorana Collaboration is assembling an array of high purity Ge detectors to search for neutrinoless double-beta decay in 76Ge. The Majorana Demonstrator is composed of 44.8 kg (29.7 kg enriched in 76Ge) of Ge detectors in total, split between two modules contained in a low background shield at the Sanford Underground Research Facility in Lead, South Dakota. The initial goals of the Demonstrator are to establish the required background and scalability of a Ge-based, next-generation, tonne-scale experiment. Following a commissioning run that began in 2015, the first detector module started physics data production in early 2016. We will discuss initial results of the Module 1 commissioning and first physics run, as well as the status and potential physics reach of the full Majorana Demonstrator experiment. The collaboration plans to complete the assembly of the second detector module by mid-2016 to begin full data production with the entire array.

The Majorana Demonstrator is an ultralow-background experiment searching for neutrinoless double-beta decay in [superscript 76]Ge. The heavily shielded array of germanium detectors, placed nearly a mile underground at the Sanford Underground Research Facility in Lead, South Dakota, also allows searches for new exotic physics. Free, relativistic, lightly ionizing particles with an electrical charge less than e are forbidden by the standard model but predicted by some of its extensions. If such particles exist, they might be detected in the Majorana Demonstrator by searching for multiple-detector events with individual-detector energy depositions down to 1 keV. This search is background-free, and no candidate events have been found in 285 days of data taking. New direct-detection limits are set for the flux of lightly ionizing particles for charges as low as e/1000., National Science Foundation (U.S.) (Grant MRI-0923142), National Science Foundation (U.S.) (Grant PHY-1003399), National Science Foundation (U.S.) (Grant PHY-1102292), National Science Foundation (U.S.) (Grant PHY1206314), National Science Foundation (U.S.) (Grant PHY-1614611), Los Alamos National Laboratory. Laboratory Directed Research and Development Program, Pacific Northwest National Laboratory (U.S.). Laboratory Directed Research and Development Program, Russian Foundation for Basic Research (Grant 15-02- 02919), Natural Sciences and Engineering Research Council of Canada (SAPIN-2017-00023), Canada Foundation for Innovation

The Majorana Demonstrator is an ultralow-background experiment searching for neutrinoless double-beta decay in ^{76}Ge. The heavily shielded array of germanium detectors, placed nearly a mile underground at the Sanford Underground Research Facility in Lead, South Dakota, also allows searches for new exotic physics. Free, relativistic, lightly ionizing particles with an electrical charge less than e are forbidden by the standard model but predicted by some of its extensions. If such particles exist, they might be detected in the Majorana Demonstrator by searching for multiple-detector events with individual-detector energy depositions down to 1 keV. This search is background-free, and no candidate events have been found in 285 days of data taking. New direct-detection limits are set for the flux of lightly ionizing particles for charges as low as e/1000.

The MAJORANA DEMONSTRATOR is an experiment constructed to search for neutrinoless double-beta decay in $^{76}$Ge and to demonstrate the feasibility to deploy a large-scale experiment in a phased and modular fashion. It consists of two modules of natural and $^{76}$Ge-enriched germanium detectors totalling 44.1 kg, operating at the 4850' level of the Sanford Underground Research Facility in Lead, South Dakota, USA. Commissioning of the experiment began in June 2015, followed by data production with the full detector array in August 2016. The ultra-low background and record energy resolution achieved by the MAJORANA DEMONSTRATOR enable a sensitive neutrinoless double-beta decay search, as well as additional searches for physics beyond the Standard Model. I will discuss the design elements that enable these searches, along with the latest results, focusing on the neutrinoless double-beta decay search. I will also discuss the current status and the future plans of the MAJORANA DEMONSTRATOR, as well as the plans for a future tonne-scale $^{76}$Ge experiment., 4 pages. Proceedings of The 39th International Conference on High Energy Physics (ICHEP2018), 4-11 July, 2018, Seoul, Korea. Submitted to Proceedings of Science

The Majorana Collaboration is operating an array of high purity Ge detectors to search for neutrinoless double-β decay in ^{76}Ge. The Majorana Demonstrator comprises 44.1 kg of Ge detectors (29.7 kg enriched in ^{76}Ge) split between two modules contained in a low background shield at the Sanford Underground Research Facility in Lead, South Dakota. Here we present results from data taken during construction, commissioning, and the start of full operations. We achieve unprecedented energy resolution of 2.5 keV FWHM at Q_{ββ} and a very low background with no observed candidate events in 9.95 kg yr of enriched Ge exposure, resulting in a lower limit on the half-life of 1.9×10^{25} yr (90% C.L.). This result constrains the effective Majorana neutrino mass to below 240-520 meV, depending on the matrix elements used. In our experimental configuration with the lowest background, the background is 4.0_{-2.5}^{+3.1} counts/(FWHM t yr).

The Majorana Collaboration is operating an array of high purity Ge detectors to search for neutrinoless double-β decay in ^{76}Ge. The Majorana Demonstrator comprises 44.1 kg of Ge detectors (29.7 kg enriched in ^{76}Ge) split between two modules contained in a low background shield at the Sanford Underground Research Facility in Lead, South Dakota. Here we present results from data taken during construction, commissioning, and the start of full operations. We achieve unprecedented energy resolution of 2.5 keV FWHM at Q_{ββ} and a very low background with no observed candidate events in 9.95 kg yr of enriched Ge exposure, resulting in a lower limit on the half-life of 1.9×10^{25} yr (90% C.L.). This result constrains the effective Majorana neutrino mass to below 240-520 meV, depending on the matrix elements used. In our experimental configuration with the lowest background, the background is 4.0_{-2.5}^{+3.1} counts/(FWHM t yr).

The Majorana Demonstrator is an array of point-contact Ge detectors fabricated from Ge isotopically enriched to 88% in 76 Ge to search for neutrinoless double beta decay. The processing of Ge for germanium detectors is a well-known technology. However, because of the high cost of Ge enriched in 76 Ge special procedures were required to maximize the yield of detector mass and to minimize exposure to cosmic rays. These procedures include careful accounting for the material; shielding it to reduce cosmogenic generation of radioactive isotopes; and development of special reprocessing techniques for contaminated solid germanium, shavings, grindings, acid etchant and cutting fluids from detector fabrication. Processing procedures were developed that resulted in a total yield in detector mass of 70%. However, none of the acid-etch solution and only 50% of the cutting fluids from detector fabrication were reprocessed. Had they been processed, the projections for the recovery yield would be between 80% and 85%. Maximizing yield is critical to justify a possible future ton-scale experiment. A process for recovery of germanium from the acid-etch solution was developed with yield of about 90%. All material was shielded or stored underground whenever possible to minimize the formation of 68 Ge by cosmic rays, which contributes background in the double-beta decay region of interest and cannot be removed by zone refinement and crystal growth. Formation of 68 Ge was reduced by a significant factor over that in natural abundance detectors not protected from cosmic rays.

This Letter reports the first scientific results from the observation of antineutrinos emitted by fission products of $^{235}$U at the High Flux Isotope Reactor. PROSPECT, the Precision Reactor Oscillation and Spectrum Experiment, consists of a segmented 4 ton $^6$Li-doped liquid scintillator detector covering a baseline range of 7-9 m from the reactor and operating under less than 1 meter water equivalent overburden. Data collected during 33 live-days of reactor operation at a nominal power of 85 MW yields a detection of 25461 $\pm$ 283 (stat.) inverse beta decays. Observation of reactor antineutrinos can be achieved in PROSPECT at 5$\sigma$ statistical significance within two hours of on-surface reactor-on data-taking. A reactor-model independent analysis of the inverse beta decay prompt energy spectrum as a function of baseline constrains significant portions of the previously allowed sterile neutrino oscillation parameter space at 95% confidence level and disfavors the best fit of the Reactor Antineutrino Anomaly at 2.2$\sigma$ confidence level., Comment: 7 pages, 5 figures; v3: Added additional supplemental files

The Majorana Collaboration is using an array of high-purity Ge detectors to search for neutrinoless double-beta decay in 76Ge. Searches for neutrinoless double-beta decay are understood to be the only viable experimental method for testing the Majorana nature of the neutrino. Observation of this decay would imply violation of lepton number, that neutrinos are Majorana in nature, and provide information on the neutrino mass. The Majorana Demonstrator comprises 44.1 kg of p-type point-contact Ge detectors (29.7 kg enriched in 76Ge) surrounded by a low-background shield system. The experiment achieved a high efficiency of converting raw Ge material to detectors and an unprecedented detector energy resolution of 2.5 keV FWHM at Q$_{\beta\beta}$. The Majorana collaboration began taking physics data in 2016. This paper summarizes key construction aspects of the Demonstrator and shows preliminary results from initial data., Comment: 5 pages, 2 figures. Proceeding for the "16th International Symposium on Capture Gamma-Ray Spectroscopy and Related Topics (CGS16)", 18-22 September 2017

The Precision Reactor Oscillation and Spectrum Experiment, PROSPECT, is designed to make both a precise measurement of the antineutrino spectrum from a highly-enriched uranium reactor and to probe eV-scale sterile neutrinos by searching for neutrino oscillations over meter-long baselines. PROSPECT utilizes a segmented $^6$Li-doped liquid scintillator detector for both efficient detection of reactor antineutrinos through the inverse beta decay reaction and excellent background discrimination. PROSPECT is a movable 4-ton antineutrino detector covering distances of 7m to 13m from the High Flux Isotope Reactor core. It will probe the best-fit point of the $\bar\nu_e$ disappearance experiments at 4$\sigma$ in 1 year and the favored regions of the sterile neutrino parameter space at more than 3$\sigma$ in 3 years. PROSPECT will test the origin of spectral deviations observed in recent $\theta_{13}$ experiments, search for sterile neutrinos, and address the hypothesis of sterile neutrinos as an explanation of the reactor anomaly. This paper describes the design, construction, and commissioning of PROSPECT and reports first data characterizing the performance of the PROSPECT antineutrino detector., Comment: 30 pages, 33 figures; updated with journal revision and reference

The Majorana Collaboration is constructing a system containing 40kg of HPGe detectors to demonstrate the feasibility and potential of a future tonne-scale experiment capable of probing the neutrino mass scale in the inverted-hierarchy region. To realize this, a major goal of the Majorana Demonstrator is to demonstrate a path forward to achieving a background rate at or below 1 cnt/(ROI-t-y) in the 4 keV region of interest around the Q-value at 2039 keV. This goal is pursued through a combination of a significant reduction of radioactive impurities in construction materials with analytical methods for background rejection, for example using powerful pulse shape analysis techniques profiting from the p-type point contact HPGe detectors technology. The effectiveness of these methods is assessed using simulations of the different background components whose purity levels are constrained from radioassay measurements.

The MAJORANA DEMONSTRATOR will search for the neutrinoless double beta decay (ββ(0ν)) of the isotope 76Ge with a mixed array of enriched and natural germanium detectors. In view of the next generation of tonne-scale germanium-based ββ(0ν)-decay searches, a major goal of the MAJORANA DEMONSTRATOR is to demonstrate a path forward to achieving a background rate at or below 1 cnt/(ROI-t-y) in the 4 keV region of interest (ROI) around the 2039-keV Q-value of the 76Ge ββ(0ν)-decay. Such a requirement on the background level significantly constrains the design of the readout electronics, which is further driven by noise and energy resolution performances. We present here the low-noise low- background front-end electronics developed for the low-capacitance p-type point contact (P-PC) germanium detectors of the MAJORANA DEMONSTRATOR. This resistive-feedback front-end, specifically designed to have low mass, is fabricated on a radioassayed fused-silica substrate where the feedback resistor consists of a sputtered thin film of high purity amorphous germanium and the feedback capacitor is based on the capacitance between gold conductive traces.

High purity germanium (HPGe) crystals will be used for the MAJORANA DEMONSTRATOR, where they serve as both the source and the detector for neutrinoless double beta decay. It is crucial for the experiment to understand the performance of the HPGe crystals. A variety of crystal properties are being investigated, including basic properties such as energy resolution, efficiency, uniformity, capacitance, leakage current and crystal axis orientation, as well as more sophisticated properties, e.g. pulse shapes and dead layer and transition layer distributions. In this paper, we will present our measurements that characterize the HPGe crystals. We will also discuss our simulation package for the detector characterization setup, and show that additional information can be extracted from data-simulation comparisons., Comment: To appear in the Proceedings of the 13th International Conference on Topics in Astroparticle and Underground Physics, TAUP 2013 (F. Avignone & W. Haxton, editors, Physics Procedia, Elsevier)

The MAJORANA Collaboration is searching for the neutrinoless double-beta decay of the nucleus Ge-76. The MAJORANA DEMONSTRATOR is an array of germanium detectors deployed with the aim of implementing background reduction techniques suitable for a tonne scale Ge-76-based search (the LEGEND collaboration). In the DEMONSTRATOR, germanium detectors operate in an ultra-pure vacuum cryostat at 80 K. One special challenge of an ultra-pure environment is to develop reliable cables, connectors, and electronics that do not significantly contribute to the radioactive background of the experiment. This paper highlights the experimental requirements and how these requirements were met for the MAJORANA DEMONSTRATOR, including plans to upgrade the wiring for higher reliability in the summer of 2018. Also described are requirements for LEGEND R&D efforts underway to meet these additional requirements., Proceedings of LRT 2017

Neutrinoless double-beta decay searches play a major role in determining the nature of neutrinos, the existence of a lepton violating process, and the effective Majorana neutrino mass. The MAJORANA Collaboration assembled an array of high purity Ge detectors to search for neutrinoless double-beta decay in Ge-76. The MAJORANA DEMONSTRATOR is comprised of 44.1 kg (29.7 kg enriched in Ge-76) of Ge detectors divided between two modules contained in a low-background shield at the Sanford Underground Research Facility in Lead, South Dakota, USA. The initial goals of the DEMONSTRATOR are to establish the required background and scalability of a Ge-based next-generation ton-scale experiment. Following a commissioning run that started in 2015, the first detector module started low-background data production in early 2016. The second detector module was added in August 2016 to begin operation of the entire array. We discuss results of the initial physics runs, as well as the status and physics reach of the full MAJORANA DEMONSTRATOR experiment., Proceedings of the MEDEX'17 meeting (Prague, May 29 - June 2, 2017)

The Majorana Collaboration is constructing the Majorana Demonstrator , an ultra-low background, 40-kg modular high purity Ge detector array to search for neutrinoless double-beta decay in 76Ge. In view of the next generation of tonne-scale Ge-based neutrinoless double-beta decay searches that will probe the neutrino mass scale in the inverted-hierarchy region, a major goal of the Demonstrator is to demonstrate a path forward to achieving a background rate at or below 1 count/tonne/year in the 4 keV region of interest around the Q-value at 2039 keV. The current status of the Demonstrator is discussed, as are plans for its completion.

A search for Pauli-exclusion-principle-violating K-alpha electron transitions was performed using 89.5 kg-d of data collected with a p-type point contact high-purity germanium detector operated at the Kimballton Underground Research Facility. A lower limit on the transition lifetime of 5.8x10^30 seconds at 90% C.L. was set by looking for a peak at 10.6 keV resulting from the x-ray and Auger electrons present following the transition. A similar analysis was done to look for the decay of atomic K-shell electrons into neutrinos, resulting in a lower limit of 6.8x10^30 seconds at 90 C.L. It is estimated that the MAJORANA DEMONSTRATOR, a 44 kg array of p-type point contact detectors that will search for the neutrinoless double-beta decay of 76-Ge, could improve upon these exclusion limits by an order of magnitude after three years of operation. Abgrall, N; Arnquist, I J; Avignone, F T; Barabash, A S; Bertrand, F E; Bradley, A W; Brudanin, V; Busch, M; Buuck, M; Caldwell, A S; Chan, Y-D; Christofferson, C D; Chu, P -H; Cuesta, C; Detwiler, J A; Dunagan, C; Efremenko, Yu; Ejiri, H; Elliott, S R; Finnerty, P S; Galindo-Uribarri, A; Gilliss, T; Giovanetti, G K; Goett, J; Green, M P; Gruszko, J; Guinn, I S; Guiseppe, V E; Henning, R; Hoppe, E W; Howard, S; Howe, M A; Jasinski, B R; Keeter, K J; Kidd, M F; Konovalov, S I; Kouzes, R T; LaFerriere, B D; Leon, J; MacMullin, J; Martin, R D; Massarczyk, R; Meijer, S J; Mertens, S; Orrell, J L; O'Shaughnessy, C; Poon, A W P; Radford, D C; Rager, J; Rielage, K; Robertson, R G H; Romero-Romero, E; Shanks, B; Shirchenko, M; Suriano, A M; Tedeschi, D; Trimble, J E; Varner, R L; Vasilyev, S; Vetter, K; Vorren, K; White, B R; Wilkerson, J F; Wiseman, C; Xu, W; Yakushev, E; Yu, C -H; Yumatov, V; Zhitnikov, I

The MAJORANA collaboration is constructing the MAJORANA DEMONSTATOR at the Sanford Underground Research Facility at the Homestake gold mine, in Lead, SD. The apparatus will use Ge detectors, enriched in isotope \nuc{76}{Ge}, to demonstrate the feasibility of a large-scale Ge detector experiment to search for neutrinoless double beta decay. The long half-life of this postulated process requires that the apparatus be extremely low in radioactive isotopes whose decays may produce backgrounds to the search. The radioassay program conducted by the collaboration to ensure that the materials comprising the apparatus are sufficiently pure is described. The resulting measurements of the radioactive-isotope contamination for a number of materials studied for use in the detector are reported., to be submitted to NIM

We report the first measurement of the total MUON flux underground at the Davis Campus of the Sanford Underground Research Facility at the 4850 ft level. Measurements were done with the Majorana Demonstrator veto system arranged in two different configurations. The measured total flux is (5.31+/-0.17) x 10^-9 muons/s/cm^2., 15 pages, 7 figures

We present new limits on exotic keV-scale physics based on 478 kg d of MAJORANA DEMONSTRATOR commissioning data. Constraints at the 90% confidence level are derived on bosonic dark matter (DM) and solar axion couplings, Pauli exclusion principle violating (PEPV) decay, and electron decay using monoenergetic peak signal-limits above our background. Our most stringent DM constraints are set for 11.8 keV mass particles, limiting $g_{Ae} 1.2 \times 10^{24}\;$yr for $e^- \rightarrow$ invisible.

The Precision Reactor Oscillation and Spectrum Experiment, PROSPECT, is designed to make a precise measurement of the antineutrino spectrum from a highly-enriched uranium reactor and probe eV-scale sterile neutrinos by searching for neutrino oscillations over meter-long distances. PROSPECT is conceived as a 2-phase experiment utilizing segmented $^6$Li-doped liquid scintillator detectors for both efficient detection of reactor antineutrinos through the inverse beta decay reaction and excellent background discrimination. PROSPECT Phase I consists of a movable 3-ton antineutrino detector at distances of 7 - 12 m from the reactor core. It will probe the best-fit point of the $\nu_e$ disappearance experiments at 4$\sigma$ in 1 year and the favored region of the sterile neutrino parameter space at $>$3$\sigma$ in 3 years. With a second antineutrino detector at 15 - 19 m from the reactor, Phase II of PROSPECT can probe the entire allowed parameter space below 10 eV$^{2}$ at 5$\sigma$ in 3 additional years. The measurement of the reactor antineutrino spectrum and the search for short-baseline oscillations with PROSPECT will test the origin of the spectral deviations observed in recent $\theta_{13}$ experiments, search for sterile neutrinos, and conclusively address the hypothesis of sterile neutrinos as an explanation of the reactor anomaly., Comment: 21 pages, 21 figures

A meter-long, 23-liter EJ-309 liquid scintillator detector has been constructed to study the light collection and pulse-shape discrimination performance of elongated scintillator cells for the PROSPECT reactor antineutrino experiment. The magnitude and uniformity of light collection and neutron/gamma discrimination power in the energy range of antineutrino inverse beta decay products have been studied using gamma and spontaneous fission calibration sources deployed along the cell long axis. We also study neutron-gamma discrimination and light collection abilities for differing PMT and reflector configurations. Key design features for optimizing MeV-scale response and background rejection capabilities are identified., Comment: 20 pages, 11 figures

The MAJORANA Collaboration is constructing the MAJORANA DEMONSTRATOR, an ultra-low background, 40-kg modular HPGe detector array to search for neutrinoless double beta decay in 76Ge. In view of the next generation of tonne-scale Ge-based 0nbb-decay searches that will probe the neutrino mass scale in the inverted-hierarchy region, a major goal of the MAJORANA DEMONSTRATOR is to demonstrate a path forward to achieving a background rate at or below 1 count/tonne/year in the 4 keV region of interest around the Q-value at 2039 keV. The background rejection techniques to be applied to the data include cuts based on data reduction, pulse shape analysis, event coincidences, and time correlations. The Point Contact design of the DEMONSTRATOR 0s germanium detectors allows for significant reduction of gamma background., 4 pages, 2 figures

Neutrinoless double-beta decay is a hypothesized process where in some even-even nuclei it might be possible for two neutrons to simultaneously decay into two protons and two electrons without emitting neutrinos. This is possible only if neutrinos are Majorana particles, i.e. fermions that are their own antiparticles. Neutrinos being Majorana particles would explicitly violate lepton number conservation, and might play a role in the matter-antimatter asymmetry in the universe. The observation of neutrinoless double-beta decay would also provide complementary information related to neutrino masses. The Majorana Collaboration is constructing the Majorana Demonstrator, a 40-kg modular germanium detector array, to search for the Neutrinoless double-beta decay of 76Ge and to demonstrate a background rate at or below 3 counts/(ROI-t-y) in the 4 keV region of interest (ROI) around the 2039 keV Q-value for 76Ge Neutrinoless double-beta decay. In this paper, we discuss the physics of neutrinoless double beta decay and then focus on the Majorana Demonstrator, including its design and approach to achieve ultra-low backgrounds and the status of the experiment., Comment: Published Version. 10 pages, 6 figures, proceedings for the 2nd Workshop on Germanium Detectors and Technologies, 14-17 September 2014, at the University of South Dakota, Vermillion, SD

The goal of the \textsc{Majorana} \textsc{Demonstrator} project is to search for 0$\nu\beta\beta$ decay in $^{76}\mathrm{Ge}$. Of all candidate isotopes for 0$\nu\beta\beta$, $^{76}\mathrm{Ge}$ has some of the most favorable characteristics. Germanium detectors are a well established technology, and in searches for 0$\nu\beta\beta$, the high purity germanium crystal acts simultaneously as source and detector. Furthermore, p-type germanium detectors provide excellent energy resolution and a specially designed point contact geometry allows for sensitive pulse shape discrimination. This paper will summarize the experiences the \textsc{Majorana} collaboration made with enriched germanium detectors manufactured by ORTEC$^{\circledR}$. The process from production, to characterization and integration in \textsc{Majorana} mounting structure will be described. A summary of the performance of all enriched germanium detectors will be given., Comment: 10 pages, 8 figures

Research reactors host a wide range of activities that make use of the intense neutron fluxes generated at these facilities. Recent interest in performing measurements with relatively low event rates, e.g. reactor antineutrino detection, at these facilities necessitates a detailed understanding of background radiation fields. Both reactor-correlated and naturally occurring background sources are potentially important, even at levels well below those of importance for typical activities. Here we describe a comprehensive series of background assessments at three high-power research reactors, including $\gamma$-ray, neutron, and muon measurements. For each facility we describe the characteristics and identify the sources of the background fields encountered. The general understanding gained of background production mechanisms and their relationship to facility features will prove valuable for the planning of any sensitive measurement conducted therein., Comment: 26 pages, 28 figures. Accepted for publication in Nuclear Instruments and Methods A. v2 incorporates minor revisions requested by NIMA

The MAJORANA Collaboration will seek neutrinoless double beta decay (0nbb) in 76Ge using isotopically enriched p-type point contact (PPC) high purity Germanium (HPGe) detectors. A tonne-scale array of HPGe detectors would require background levels below 1 count/ROI-tonne-year in the 4 keV region of interest (ROI) around the 2039 keV Q-value of the decay. In order to demonstrate the feasibility of such an experiment, the MAJORANA DEMONSTRATOR, a 40 kg HPGe detector array, is being constructed with a background goal of, 6 pages, 7 figures, 2 tables. LRT 2015 Proceedings