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Significance Pre-participation physical evaluation and its vision screenings have been the mainstay of medical clearance for competitive play for decades. The ability of screening to address athlete's sports-specific vision needs is unknown. Methods Fifty-eight intercollegiate football players consented to participate in a comprehensive, sports-specific eye examination in addition to the standard pre-participation vision screening. Sensitivity, specificity, and positive and negative predictive values were determined for screening's ability to detect athletes whose vision might improve with correction, athletes who had significant ocular findings that impact safety, and either of the two conditions together. The effect no recent eye examination added to pre-participation vision screening results was evaluated for change in screening yield. Descriptive statistics of the cohort and associations with no recent comprehensive eye examination were generated. Results The pre-participation vision screening was able to identify three athletes not meeting visual acuity requirements for medical clearance to play without a comprehensive assessment. A failed screening was poorly able to identify athletes who might benefit from improved acuity (sensitivity, 9.1%; specificity, 100%), have sports-specific significant ocular findings (sensitivity, 10.5%; specificity, 97.3%), or have either together (sensitivity, 7.5%; specificity, 100%). Sixty percent (33/55) of athletes reported never having a comprehensive examination or one within the last 10 years. Fifty-eight percent (34/58) had improved best-corrected visual acuity after comprehensive examination, and 81% (47/58) had improved acuity or a sports-specific significant finding. Conclusions The pre-participation vision screening was largely able to identify athletes meeting the minimum visual acuity requirement for athlete clearance. It poorly identified those who might benefit from improved vision with refractive correction and those in whom sport-specific significant eye findings were noted. Comprehensive eye care had a clear benefit for the majority of athletes tested. This benefit needs to be balanced with the potential added costs and time constraints to players and athletic department staff.

Both aberrant vascular reactivity to acute cardiovascular stress and epigenetic mechanisms such as microRNA (miR) may underlie the increased propensity for African Americans (AA) to develop cardiovascular disease. This study assessed racial differences in acute induced endothelial inflammation and related miRs. Cultured human umbilical vein endothelial cells (HUVECs) derived from AA and Caucasian Americans (CA) were exposed to influenza vaccine to determine changes in inflammatory markers, endothelial nitric oxide synthase (eNOS), and miR expression/release. Endothelial function [flow-mediated dilation (FMD)], circulating IL-6, and circulating miR were also measured in young, healthy AA and CA individuals before and after receiving the influenza vaccine. There were no significant racial differences in any parameters at baseline. The vaccine induced increases in IL-6 release (24%

The Las Vegas Formation (LVF) is a well-characterized sequence of groundwater discharge (GWD) deposits exposed in and around the Las Vegas Valley in southern Nevada. Nearly monolithologic bedrock surrounds the valley, which provides an excellent opportunity to test the hypothesis that GWD deposits include an aeolian component. Mineralogical data indicate that the LVF sediments are dominated by carbonate minerals, similar to the local bedrock, but silicate minerals are also present. The median particle size is ~35 μm, consistent with modern dust in the region, and magnetic properties contrast strongly with local bedrock, implying an extralocal origin. By combining geochemical data from the LVF sediments and modern dust, we found that an average of ~25% of the LVF deposits were introduced by aeolian processes. The remainder consists primarily of authigenic groundwater carbonate as well as minor amounts of alluvial material and soil carbonate. Our data also show that the aeolian sediments accumulated in spring ecosystems in the Las Vegas Valley in a manner that was independent of both time and the specific hydrologic environment. These results have broad implications for investigations of GWD deposits located elsewhere in the southwestern U.S. and worldwide.

Recent studies have shown the oxygen isotopic composition (δ18O) of modern terrestrial gastropod shells is determined largely by the δ18O of precipitation. This implies that fossil shells could be used to reconstruct the δ18O of paleo-precipitation as long as the isotopic system, including the hydrologic pathways of the local watershed and the gastropod systematics, is well understood. In this study, we measured the δ18O values of 456 individual gastropod shells collected from paleowetland deposits in the San Pedro Valley, Arizona that range in age from ca. 29.1 to 9.8 ka. Isotopic differences of up to 2‰ were identified among the four taxa analyzed (Succineidae, Pupilla hebes, Gastrocopta tappaniana, and Vallonia gracilicosta), with Succineidae shells yielding the highest values and V. gracilicosta shells exhibiting the lowest values. We used these data to construct a composite isotopic record that incorporates these taxonomic offsets, and found shell δ18O values increased by ~4‰ between the last glacial maximum and early Holocene, which is similar to the magnitude, direction, and rate of isotopic change recorded by speleothems in the region. These results suggest the terrestrial gastropods analyzed here may be used as a proxy for past climate in a manner that is complementary to speleothems, but potentially with much greater spatial coverage.

The Eglington fault is one of several intrabasinal faults in the Las Vegas Valley, Nevada, USA, and is the only one recognized as a source for significant earthquakes. Its broad warp displaces Late Pleistocene spring deposits of the Las Vegas Formation, which record hydrologic fluctuations that occurred in response to millennial- and submillennial-scale climate oscillations throughout the late Quaternary. The sediments allow us to constrain the timing of displacement on the Eglington fault and identify hydrologic changes that are temporally coincident with that event. The fault deforms deposits that represent widespread marshes that filled the valley between ca. 31.7 and 27.6 ka. These marshes desiccated abruptly in response to warming and groundwater lowering during Dansgaard-Oeschger (D-O) events 4 and 3, resulting in the formation of a pervasive, hard carbonate cap by 27.0 ka. Vertical offset by as much as 4.2 m occurred after the cap hardened, and most likely after younger marshes desiccated irreversibly due to a sudden depression of the water table during D-O event 2, beginning at 23.3 ka. The timing of displacement is further constrained to before 19.5 ka as evidenced by undeformed spring deposits that are inset into the incised topography of the warp. Coulomb stress calculations validate the hypothesis that the substantial groundwater decline during D-O event 2 unclamped the fault through unloading of vertical stress of the water column. The synchroneity of this abrupt hydrologic change and displacement of the Eglington fault suggests that climatically modulated tectonics operated in the Las Vegas Valley during the late Quaternary.

Madsen et al . question the reliability of calibrated radiocarbon ages associated with human footprints discovered recently in White Sands National Park, New Mexico, USA. On the basis of the geologic, hydrologic, stratigraphic, and chronologic evidence, we maintain that the ages are robust and conclude that the footprints date to between ~23,000 and 21,000 years ago. Madsen et al . ( 1 ) question the veracity of calibrated radiocarbon ages used to constrain the antiquity of human trackways discovered recently at White Sands National Park (WHSA) Locality 2, New Mexico, USA ( 2 ). The ages were derived from seeds of the aquatic plant Ruppia cirrhosa , which they suggest may suffer from hard-water (or reservoir) effects, making them too old, potentially by thousands of years. We were well aware of this possibility, investigated it, and presented several lines of evidence that argued against such a problem. Here we respond to each of their four primary points.

The Younger Dryas (YD) climate event is the preeminent example of abrupt climate change in the recent geologic past. Climate conditions during the YD were spatially complex, and high-resolution sediment cores in the North Atlantic, western Europe, and East Asia have revealed it unfolded in two distinct stages, including an initial stable climatic period between ~ 12.9 and 12.2 ka associated with a weakened Atlantic Meridional Overturning Circulation (AMOC) and a second phase characterized by variable conditions until 11.7 ka as the AMOC recovered. Decades of investigations into the climate of western North America during the YD have failed to identify this stepped phenomenon. Here we present hydroclimate data from paleospring deposits in Death Valley National Park (California, USA) that demonstrate unequivocal evidence of two-stage partitioning within the YD event. High groundwater levels supported persistent and long-lived spring ecosystems between ~ 13.0 and 12.2 ka, which were immediately replaced by alternating wet and dry environments until ~ 11.8 ka. These results establish the mid-YD climate transition extended into western North America at approximately the same time it was recorded by hydrologic systems elsewhere in the Northern Hemisphere and show that even short-lived changes in the AMOC can have profound consequences for ecosystems worldwide.

Early footsteps in the Americas Despite a plethora of archaeological research over the past century, the timing of human migration into the Americas is still far from resolved. In a study of exposed outcrops of Lake Otero in White Sands National Park in New Mexico, Bennett et al . reveal numerous human footprints dating to about 23,000 to 21,000 years ago. These finds indicate the presence of humans in North America for approximately two millennia during the Last Glacial Maximum south of the migratory barrier created by the ice sheets to the north. This timing coincided with a Northern Hemispheric abrupt warming event, Dansgaard-Oeschger event 2, which drew down lake levels and allowed humans and megafauna to walk on newly exposed surfaces, creating tracks that became preserved in the geologic record. —AMS

Pre-participation physical evaluation and its vision screenings have been the mainstay of medical clearance for competitive play for decades. The ability of screening to address athlete's sports-specific vision needs is unknown.Fifty-eight intercollegiate football players consented to participate in a comprehensive, sports-specific eye examination in addition to the standard pre-participation vision screening. Sensitivity, specificity, and positive and negative predictive values were determined for screening's ability to detect athletes whose vision might improve with correction, athletes who had significant ocular findings that impact safety, and either of the two conditions together. The effect no recent eye examination added to pre-participation vision screening results was evaluated for change in screening yield. Descriptive statistics of the cohort and associations with no recent comprehensive eye examination were generated.The pre-participation vision screening was able to identify three athletes not meeting visual acuity requirements for medical clearance to play without a comprehensive assessment. A failed screening was poorly able to identify athletes who might benefit from improved acuity (sensitivity, 9.1%; specificity, 100%), have sports-specific significant ocular findings (sensitivity, 10.5%; specificity, 97.3%), or have either together (sensitivity, 7.5%; specificity, 100%). Sixty percent (33/55) of athletes reported never having a comprehensive examination or one within the last 10 years. Fifty-eight percent (34/58) had improved best-corrected visual acuity after comprehensive examination, and 81% (47/58) had improved acuity or a sports-specific significant finding.The pre-participation vision screening was largely able to identify athletes meeting the minimum visual acuity requirement for athlete clearance. It poorly identified those who might benefit from improved vision with refractive correction and those in whom sport-specific significant eye findings were noted. Comprehensive eye care had a clear benefit for the majority of athletes tested. This benefit needs to be balanced with the potential added costs and time constraints to players and athletic department staff.

New photochemically removable protecting groups have been developed based on classical nitrobenzyl compounds modified by the inclusion of a pentadienyl group. It serves to trap through an internal Diels-Alder reaction the nitroso group produced as part of the photochemical deprotection process, preventing its further photochemistry or chemical reactions with nucleophiles.

Repeated exposure to a high-fat meal triggers inflammation and oxidative stress, contributing to the onset of cardiometabolic diseases. Regular exercise prevents cardiometabolic diseases and a prior bout of acute endurance exercise can counteract the detrimental cardiovascular effects of a subsequent high-fat meal. Circulating microRNAs (ci-miRs) are potential mediators of these vascular effects through regulation of gene expression at the posttranscriptional level. Therefore, we investigated the expression of ci-miRs related to vascular function (miR-21, miR-92a, miR-126, miR-146a, miR-150, miR-155, miR-181b, miR-221, miR-222) in plasma from healthy, recreationally to highly active, Caucasian adult men after a high-fat meal with (EX) and without (CON) a preceding bout of cycling exercise. Ci-miR-155 was the only ci-miR for which there was a significant interaction effect of high-fat meal and exercise (p=0.050). Ci-miR-155 significantly increased in the CON group at two (p=0.007) and four hours (p=0.010) after the high-fat meal test, whereas it significantly increased in the EX group only four hours after the meal (p=0.0004). There were significant main effects of the high-fat meal on ci-miR-21 (p=0.01), ci-miR-126 (p=0.02), ci-miR-146a (p=0.02), ci-miR-181b (p=0.02), and ci-miR-221 (p=0.008). Collectively, our results suggest that prior exercise does not prevent high-fat meal-induced increases in vascular-related ci-miRs.

Background Skeletal muscle atrophy, whether caused by chronic disease, acute critical illness, disuse or aging, is characterized by tissue-specific decrease in oxidative capacity and broad alterations in metabolism that contribute to functional decline. However, the underlying mechanisms responsible for these metabolic changes are largely unknown. One of the most highly upregulated genes in atrophic muscle is AMP deaminase 3 (AMPD3: AMP → IMP + NH3), which controls the content of intracellular adenine nucleotides (AdN; ATP + ADP + AMP). Given the central role of AdN in signaling mitochondrial gene expression and directly regulating metabolism, we hypothesized that overexpressing AMPD3 in muscle cells would be sufficient to alter their metabolic phenotype similar to that of atrophic muscle. Methods AMPD3 and GFP (control) were overexpressed in mouse tibialis anterior (TA) muscles via plasmid electroporation and in C2C12 myotubes using adenovirus vectors. TA muscles were excised one week later, and AdN were quantified by UPLC. In myotubes, targeted measures of AdN, AMPK/PGC-1α/mitochondrial protein synthesis rates, unbiased metabolomics, and transcriptomics by RNA sequencing were measured after 24 h of AMPD3 overexpression. Media metabolites were measured as an indicator of net metabolic flux. At 48 h, the AMPK/PGC-1α/mitochondrial protein synthesis rates, and myotube respiratory function/capacity were measured. Results TA muscles overexpressing AMPD3 had significantly less ATP than contralateral controls (−25%). In myotubes, increasing AMPD3 expression for 24 h was sufficient to significantly decrease ATP concentrations (−16%), increase IMP, and increase efflux of IMP catabolites into the culture media, without decreasing the ATP/ADP or ATP/AMP ratios. When myotubes were treated with dinitrophenol (mitochondrial uncoupler), AMPD3 overexpression blunted decreases in ATP/ADP and ATP/AMP ratios but exacerbated AdN degradation. As such, pAMPK/AMPK, pACC/ACC, and phosphorylation of AMPK substrates, were unchanged by AMPD3 at this timepoint. AMPD3 significantly altered 191 out of 639 detected intracellular metabolites, but only 30 transcripts, none of which encoded metabolic enzymes. The most altered metabolites were those within purine nucleotide, BCAA, glycolysis, and ceramide metabolic pathways. After 48 h, AMPD3 overexpression significantly reduced pAMPK/AMPK (−24%), phosphorylation of AMPK substrates (−14%), and PGC-1α protein (−22%). Moreover, AMPD3 significantly reduced myotube mitochondrial protein synthesis rates (−55%), basal ATP synthase-dependent (−13%), and maximal uncoupled oxygen consumption (−15%). Conclusions Increased expression of AMPD3 significantly decreased mitochondrial protein synthesis rates and broadly altered cellular metabolites in a manner similar to that of atrophic muscle. Importantly, the changes in metabolites occurred prior to reductions in AMPK signaling, gene expression, and mitochondrial protein synthesis, suggesting metabolism is not dependent on reductions in oxidative capacity, but may be consequence of increased AMP deamination. Therefore, AMP deamination in skeletal muscle may be a mechanism that alters the metabolic phenotype of skeletal muscle during atrophy and could be a target to improve muscle function during muscle wasting.

Portable luminescence readers are exciting new tools that have the potential to rapidly determine the age structure of late Quaternary stratigraphic columns. This is important because high-resolution age profiling can reveal details about the temporal dynamics of climate cause and ecosystem effect, often while researchers are still in the field. In this paper, we compare new portable luminescence reader measurements of total photon counts with a suite of robust, highly resolved ages from middle to late Pleistocene-age paleowetland deposits in the Las Vegas Valley of southern Nevada. Our results show that total photon counts correlate with age, with a quadratic equation providing the best fit to the data. Significant scatter is present in the data, which is likely the result of dose rate variations, multiple sediment sources, and transport mechanisms that include both eolian and fluvial processes. The observed scatter can be reduced significantly using a simple pretreatment procedure involving a 250 μm sieve and neodymium hand magnet to normalize particle sizes and remove magnetic grains. Following this treatment, age estimates based on the reader measurements have an average error of 30 ± 18% when compared against known ages. These findings confirm that portable reader measurements scale with age in paleowetland deposits, allowing its use in establishing rapid, albeit approximate, chronologies for these deposits throughout the American Southwest.

One of the enduring questions in the field of paleohydrology is how quickly desert wetland ecosystems responded to past episodes of abrupt climate change. Recent investigations in the Las Vegas Valley of southern Nevada have revealed that wetlands expanded and contracted on millennial and sub-millennial timescales in response to changes in climate during the late Quaternary. Here, we evaluate geologic evidence from multiple localities in the Mojave Desert and southern Great Basin that suggests the response of wetland systems to climate change is even faster, occurring at centennial, and possibly decadal, timescales. Paleowetland deposits at Dove Springs Wash, Mesquite Springs, and Little Dixie Wash, California, contain evidence of multiple wet and dry cycles in the form of organic-rich black mats, representing periods of past groundwater discharge and wet conditions, interbedded with colluvial, alluvial, and aeolian sediments, each representing dry conditions. Many of these wet-dry cycles date to within the Younger Dryas (YD) chronozone (12.9–11.7 ka), marking the first timeintra-YD hydrologic variability has been documented in paleowetland deposits. Our results illustrate that desert wetland ecosystems are exceptionally sensitive to climate change and respond to climatic perturbations on timescales that are relevant to human society.

A middle to late Pleistocene sedimentary sequence in the upper Las Vegas Wash, north of Las Vegas, Nevada, has yielded the largest open-site Rancholabrean vertebrate fossil assemblage in the southern Great Basin and Mojave Deserts. Recent paleontologic field studies have led to the discovery of hundreds of fossil localities and specimens, greatly extending the geographic and temporal footprint of original investigations in the early 1960s. The significance of the deposits and their entombed fossils led to the preservation of 22,650 acres of the upper Las Vegas Wash as Tule Springs Fossil Beds National Monument. These discoveries also warrant designation of the assemblage as a local fauna, named for the site of the original paleontologic studies at Tule Springs. The large mammal component of the Tule Springs local fauna is dominated by remains of Mammuthus columbi as well as Camelops hesternus , along with less common remains of Equus (including E. scotti ) and Bison . Large carnivorans including Canis dirus , Smilodon fatalis , and Panthera atrox are also recorded. Micromammals, amphibians, lizards, snakes, birds, invertebrates, plant macrofossils, and pollen also occur in the deposits and provide important and complementary paleoenvironmental information. The fauna occurs within the Las Vegas Formation, an extensive and stratigraphically complex sequence of groundwater discharge deposits that represent a mosaic of desert wetland environments. Radiometric and luminescence dating indicates the sequence spans the last ∼570 ka, and records hydrologic changes in a dynamic and temporally congruent response to northern hemispheric abrupt climatic oscillations. The vertebrate fauna occurs in multiple stratigraphic horizons in this sequence, with ages of the fossils spanning from ∼100 to ∼12.5 ka.

Tule Springs Fossil Beds National Monument (TUSK) preserves 22,650 acres of the upper Las Vegas Wash in the northern Las Vegas Valley (Nevada, USA). TUSK is home to extensive and stratigraphically complex groundwater discharge (GWD) deposits, called the Las Vegas Formation, which represent springs and desert wetlands that covered much of the valley during the late Quaternary. The GWD deposits record hydrologic changes that occurred here in a dynamic and temporally congruent response to abrupt climatic oscillations over the last ~300 ka (thousands of years). The deposits also entomb the Tule Springs Local Fauna (TSLF), one of the most significant late Pleistocene (Rancholabrean) vertebrate assemblages in the American Southwest. The TSLF is both prolific and diverse, and includes a large mammal assemblage dominated by Mammuthus columbi and Camelops hesternus . Two (and possibly three) distinct species of Equus , two species of Bison , Panthera atrox , Smilodon fatalis , Canis dirus , Megalonyx jeffersonii , and Nothrotheriops shastensis are also present, and newly recognized faunal components include micromammals, amphibians, snakes, and birds. Invertebrates, plant macrofossils, and pollen also occur in the deposits and provide important and complementary paleoenvironmental information. This field compendium highlights the faunal assemblage in the classic stratigraphic sequences of the Las Vegas Formation within TUSK, emphasizes the significant hydrologic changes that occurred in the area during the recent geologic past, and examines the subsequent and repeated effect of rapid climate change on the local desert wetland ecosystem.

A new species of mastodon from the Pleistocene of western North America, Mammut pacificus sp. nov. is herein recognized, with specimens identified throughout California and from two localities in southern Idaho. This new taxon differs from the contemporaneous M. americanum in having narrower teeth, most prominently in M3/m3, as well as six sacral vertebrae, femur with a proportionally greater mid-shaft diameter, and no mandibular tusks at any growth stage. All known Pleistocene Mammut remains from California are consistent with our diagnosis of M. pacificus, which indicates that M. americanum was not present in California.

Post-traumatic stress disorder (PTSD) and traumatic brain injury (TBI) remain central topics in institutions’ efforts to address student veteran needs. From the authors’ experiences with student veterans, they present the five principles of effective instructional practice identified by the National Research Council (NRC) within the context of college teaching. The purpose of this article is to inform faculty of the benefits of implementing these principles, thereby increasing the likelihood of academic success for all students, especially student veterans.