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4. TOWARDS A DIAGNOSIS OF NON-CELIAC GLUTEN SENSITIVITY: the contribution of metabolomics for monitoring metabolites produced by in vitro digestates of bread

Author

Zhour, Houssein, Genot, Mélany, Peyron, Marie-Agnes, Petera, Melanie, Sante-Lhoutellier, Veronique, Durand, Stephanie, Centeno, Delphine, Bancel, Emmanuelle, Ravel, Catherine, Pujos-Guillot, Estelle, Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-Clermont, MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), RFMF, and Réseau Français de Métabolomique et Fluxomique

Subjects
[STAT]Statistics [stat], in vitro digestion, diagnosis, [SDV]Life Sciences [q-bio], [CHIM]Chemical Sciences, Non-Celiac Gluten Sensitivity, methodology, [INFO]Computer Science [cs], NCGS, metabolomics, Gluten
Abstract

International audience; Body fluid metabolomics is a large-scale approach allowing exploring the mechanisms that might underlie specific diseases or sensitivity to processed foods, and identifying associated biomarkers for diagnostics or stratification. Over the past decade, the non-celiac gluten sensitivity (NCGS) is more and more self-diagnosed, which makes the gluten-free diet more frequent, without objective clinical criteria. In fact, because of a lack of clinical indicators, NCGS is poorly understood and challenging to diagnose in contrast to celiac disease. Therefore, finding biomarkers associated with this phenotype is critical for an accurate diagnosis and innovative patient management.To understand the relationship between bread digestion mechanisms and the occurrence of NCGS, a recent approach with in vitro investigation was applied to study the overall digestive process of different breads, combining tools from the oral step thanks to the AM2 masticator apparatus, until the end of digestion thanks to a dynamic digester (DIDGI©) mimicking the physiology of the adult gastrointestinal tract "GIT". One objective in this study was to monitor metabolites produced by in vitro digestates using an untargeted metabolomics approach.In this study, we will outline the methodological strategy taken from preparation of the stomach and intestinal digestates, to acquisition, processing, and annotation of the LC-HRMS data.Interestingly, the first results show fluctuations in certain metabolites identified according to the type of bread digested. This reveals the impact of type of bread on the digestibility and allowed us to emphasize the contribution of metabolomic approach for monitoring the metabolites produced by in vitro digestates.

Published
2023

7. Bread wheat (T. aestivum) variability: Phenotypic and genotypic data from 75 varieties

Author

Lavoignat, Mélanie, primary, Bancel, Emmanuelle, additional, Rimbert, Hélène, additional, Bagnon, Sandrine, additional, Benigna, Michaël, additional, Chassin, Alain, additional, Berges, Sandrine, additional, Faye, Annie, additional, Heumez, Emmanuel, additional, Perrochon, Sibille, additional, Rhazi, Larbi, additional, Valluis, Bernard, additional, Souply, Flavie, additional, Leroux, Marie Cécile, additional, Giraudeau, Pascal, additional, and Ravel, Catherine, additional

Published
2023
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8. Differences in bread protein digestibility traced to wheat cultivar traits

Author

Lavoignat, Mélanie, primary, Denis, Sylvain, additional, Faye, Annie, additional, Halupka, Laura, additional, Perrochon, Sibille, additional, Rhazi, Larbi, additional, Giraudeau, Pascal, additional, Déjean, Sébastien, additional, Branlard, Gérard, additional, Bancel, Emmanuelle, additional, and Ravel, Catherine, additional

Published
2022
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9. Sulfur in determining seed protein composition: present understanding of its interaction with abiotic stresses and future directions.

Author

Bonnot, Titouan, Bachelet, Fanélie, Boudet, Julie, Signor, Christine Le, Bancel, Emmanuelle, Vernoud, Vanessa, Ravel, Catherine, and Gallardo, Karine

Subjects
SEED proteins, COMPOSITION of seeds, LEGUMES, DIETARY proteins, ABIOTIC stress, SULFUR
Abstract

Improving and stabilizing the quality of seed proteins are of growing interest in the current food and agroecological transitions. Sulfur is a key determinant of this quality since it is essential for the synthesis of sulfur-rich proteins in seeds. A lack of sulfur provokes drastic changes in seed protein composition, negatively impacting the nutritional and functional properties of proteins, and leading in some cases to diseases or health problems in humans. Sulfur also plays a crucial role in stress tolerance through the synthesis of antioxidant or protective molecules. In the context of climate change, questions arise regarding the trade-off between seed yield and seed quality with respect to sulfur availability and use by crops that represent important sources of proteins for human nutrition. Here, we review recent work obtained in legumes, cereals, as well as in Arabidopsis, that present major advances on: (i) the interaction between sulfur nutrition and environmental or nutritional stresses with regard to seed yield and protein composition; (ii) metabolic pathways that merit to be targeted to mitigate negative impacts of environmental stresses on seed protein quality; and (iii) the importance of sulfur homeostasis for the regulation of seed protein composition and its interplay with seed redox homeostasis. [ABSTRACT FROM AUTHOR]

Published
2023
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10. GlutNsafe : « Vers la sélection de variétés de blés au gluten plus digestible ? »

Author

Lavoignat, Mélanie, Denis, Sylvain, Faye, Annie, Halupka, Laura, Perrochon, Sibille, Rhazi, Larbi, Dejean, Sébastien, Giraudeau, Pascal, Branlard, Gerard, Ravel, Catherine, Bancel, Emmanuelle, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), AgroParisTech, Microbiologie Environnement Digestif Santé (MEDIS), CERELAB (CERELAB), UniLaSalle, Institut de Mathématiques de Toulouse UMR5219 (IMT), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Union Française des Semenciers (UFS), and Fonds de soutien à l'obtention végétale

Subjects
blé, gluten, [SDV]Life Sciences [q-bio]
Abstract

International audience; A l'occasion de sa 8ème Rencontre scientifique, le FSOV propose "une journée entièrement consacrée à la restitution des résultats des 15 programmes de recherche financés dans le cadre de l’appel à propositions du FSOV 2016"

Published
2022

12. Intégration de données pour caractériser la digestibilité des protéines du pain

Author

m, lavoignat, Bancel, Emmanuelle, ALRIC, Monique, Bagnon, S, Benigna, M, Chassin, A, Berges, S, Denis, Sylvain, Faye, A, Heumez, E., Dymarski, Pierre-Yves, Halupka, L, Perrochon, Sibille, Rhazi, L, Valluis, B, Giraudeau, P., Ravel, C, Microbiologie Environnement Digestif Santé (MEDIS), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

13. Intégration de données pour caractériser la digestibilité des protéines du pain

Author

Lavoignat, Mélanie, Bancel, Emmanuelle, Alric, Monique, Bagnon, Sandrine, Benigna, Michael, Chassin, Alain, Berges, Sandrine, Denis, Sylvain, Faye, Annie, Heumez, Emmanuel, Dymarski, Pierre-Yves, Halupka, Laura, Perrochon, Sibille, Rhazi, Larbi, Valluis, Bernard, Souply, Flavie, Leroux, Marie-Cécile, Giraudeau, Pascal, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Microbiologie Environnement Digestif Santé (MEDIS), Qualtech, IFBM, Unité Expérimentale PHénotypage Au Champ des Céréales (UE1375) (PHACC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale Grandes Cultures Innovation Environnement - Picardie (GCIE), CERELAB® La Sucrerie, Partenaires INRAE, Interact, UniLaSalle, 60 000 Beauvais, France, Association Nationale de la Meunerie Française (ANMF), Union Française des Semenciers (UFS), and Institut Français de la Brasserie et de la Malterie (IFBM)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

14. Quels caractères améliorer pour un gluten plus digeste

Author

Ravel, Catherine, Bancel, Emmanuelle, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Arvalis - Institut du végétal, and Bernard, Nathalie

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

15. Omics Data Reveal Putative Regulators of Einkorn Grain Protein Composition under Sulfur Deficiency

Author

Bonnot, Titouan, primary, Martre, Pierre, additional, Hatte, Victor, additional, Dardevet, Mireille, additional, Leroy, Philippe, additional, Bénard, Camille, additional, Falagán, Natalia, additional, Martin-Magniette, Marie-Laure, additional, Deborde, Catherine, additional, Moing, Annick, additional, Gibon, Yves, additional, Pailloux, Marie, additional, Bancel, Emmanuelle, additional, and Ravel, Catherine, additional

Published
2020
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16. Proteomic Data Integration Highlights Central Actors Involved in Einkorn (Triticum monococcum ssp. monococcum) Grain Filling in Relation to Grain Storage Protein Composition

Author

Bancel, Emmanuelle, Bonnot, Titouan, Davanture, Marlene, Alvarez, David, Zivy, Michel, Martre, Pierre, Déjean, Sébastien, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Génétique Diversité et Ecophysiologie des Céréales - Clermont Auvergne (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), AMERIBER : Amérique latine, Pays ibériques (AMERIBER), Université Bordeaux Montaigne, Génétique Végétale (GV), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Institut de Mathématiques de Toulouse UMR5219 (IMT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Triticum monococcum, endosperme, Nitrogen, albumine globuline, Ingénierie des aliments, intégration des données, Proteomic, food and beverages, Plant Science, Albumin-globulin, Data integration, Grain development, Storage protein, Sulfur, approche protéomique, développement du grain, Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, grain de blé
Abstract

International audience; Albumins and globulins (AGs) of wheat endosperm represent about 20% of total grain proteins. Some of these physiologically active proteins can influence the synthesis of storage proteins (SPs) (gliadins and glutenins) and consequently, rheological properties of wheat flour and processing. To identify such AGs, data, (published by Bonnot et al., 2017) concerning abundance in 352 AGs and in the different seed SPs during grain filling and in response to different nitrogen (N) and sulfur (S) supply, were integrated with mixOmics R package. Relationships between AGs and SPs were first unraveled using the unsupervised method sparse Partial Least Square, also known as Projection to Latent Structure (sPLS). Then, data were integrated using a supervised approach taking into account the nutrition and the grain developmental stage. We used the block.splda procedure also referred to as DIABLO (Data Integration Analysis for Biomarker discovery using Latent variable approaches for Omics studies). These approaches led to the identification of discriminant and highly correlated features from the two datasets (AGs and SPs) which are not necessarily differentially expressed during seed development or in response to N or S supply. Eighteen AGs were correlated with the quantity of SPs per grain. A statistical validation of these proteins by genetic association analysis confirmed that 5 out of this AG set were robust candidate proteins able to modulate the seed SP synthesis. In conclusion, this latter result confirmed that the integrative strategy is an adequate way to reduce the number of potentially relevant AGs for further functional validation.

Published
2019

17. La recherche variétale pour une meilleure tolérance au gluten

Author

Bancel, Emmanuelle, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Céréales Vallée Nutravita., Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], approche variétale, gluten, [SDV]Life Sciences [q-bio], tolérance
Abstract

La recherche variétale pour une meilleure tolérance au gluten. Séminaire technique Céréales Vallée. Gluten, Mieux le connaitre pour (ré)agir

Published
2018

18. Projet GlutN : comprendre la sensibilité au gluten et proposer des produits adaptés à base de blé

Author

Ravel, Catherine, Bancel, Emmanuelle, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Céréales Vallée., ProdInra, Archive Ouverte, Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)

Subjects
[SDV] Life Sciences [q-bio], blé, wheat, gluten, [SDV]Life Sciences [q-bio], sensitivity, sensibilité
Abstract

Projet GlutN : comprendre la sensibilité au gluten et proposer des produits adaptés à base de blé. Séminaire technique Céréales Vallée. Gluten Mieux le connaitre pour (ré)agir

Published
2018

19. Proteomic data integration to highlight central actors involved in einkorn grain filling in relation to quality

Author

Bancel, Emmanuelle, Bonnot, Titouan, Martre, Pierre, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Fédération Italienne des Sciences de la Vie (FISSV)., Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], thorns, quality, qualité, [SDV]Life Sciences [q-bio], épine, remplissage du grain, seed filling, approche protéomique
Abstract

Proteomic data integration to highlight central actors involved in einkorn grain filling in relation to quality. 3. International Plant Proteomics Organization World Congress

Published
2018

20. Using c-terminomics of grain storage proteins to explore wheat quality

Author

Miranda, Julio, Bancel, Emmanuelle, Viala, Didier, Chambon, Christophe, Capelo, Jose, Branlard, Gerard, Ravel, Catherine, Igrejas, Gilberto, Function Genomic and Prote Unity, Univ Tras Os Montes and Alto Douro, Universidade Nova de Lisboa (NOVA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Departamento de Química (DQ), Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), COST FA 1306., Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
protéine de stockage, [SDV]Life Sciences [q-bio], qualité du blé
Abstract

51, p. 58; Using c-terminomics of grain storage proteins to explore wheat quality. COST FA1306 final meeting

Published
2018

21. Proteomic changes occuring in einkorn (Triticum monoccocum) grain during filling stages of development

Author

Bancel, Emmanuelle, Bonnot, Titouan, Davanture, Marlène, Pailloux, Marie, Zivy, Michel, Martre, Pierre, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Informatique, de Modélisation et d'Optimisation des Systèmes (LIMOS), Ecole Nationale Supérieure des Mines de St Etienne (ENSM ST-ETIENNE)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Ecole Nationale Supérieure des Mines de St Etienne-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Ecole Nationale Supérieure des Mines de St Etienne-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), and Ecole Nationale Supérieure des Mines de St Etienne-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)

Subjects
blé, wheat, proteome, globuline, [SDV]Life Sciences [q-bio], food and beverages, albumins globulins, albumine, co-accumulation network, ovalbumin, grain, globulin, protéome
Abstract

ISBN: 978-2-9563873-0-5ISBN: 978-2-9563873-0-5; Albumins and globulins of wheat endosperm represent about 20% of total grain proteins. Nutritionally, the albumins and globulins (non-glutens) have a very good amino acid balance. Many of these proteins are enzymes involved in metabolic activity. Most of these physiologically active proteins can also influence the processing and rheological properties of wheat flour.Incomplete knowledge of these enzymes and related proteins present in the endosperm of wheat limits our understanding of the biochemical events taking place in the grain during the first stages of its development. So it is necessary to study and evaluate qualitative and quantitative changes occurring during this process. To reach this objective, our study is based on Triticum monococcum (einkorn), a diploid wheat species, used as a model species of bread wheat (T. aestivum).We used a proteomic approach to identify the key actors during the filling stages of development in einkorn grain. Five stages were studied (200 to 600°Cd). Large-scale analysis using the gel free proteomic approach allowed us to identify a total of 2,475 proteins. Among these proteins, 512 (20.7%) were accurately quantified, 488 of them presented changes related to stage development, highlighting this dynamic process (Bonnot et al, 2017).Hierarchical cluster analysis revealed four major expression profiles throughout grain development. The classification of proteins identified by MS/MS in 17 different biochemical functions and nine molecular functions provided a picture of the temporal cellular processes involved. Several proteins differentially expressed and associated with grain development and/or quality were also shown.A co-accumulation network of all differentially expressed proteins mainly centred on enzymes involved in carbon and protein metabolism was finally established.

Published
2018

22. Following the c-terminomics of wheat storage proteins– a new quality field to explore

Author

Miranda, Julio, Bancel, Emmanuelle, Viala, Didier, Chambon, Christophe, Capelo, José L, Branlard, Gerard, Ravel, Catherine, Igrejas, Gilberto, Universidade de Trás-os-Montes e Alto Douro, Universidade Nova de Lisboa (NOVA), Institut National de la Recherche Agronomique (INRA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Qualité des Produits Animaux (QuaPA), University of Lisbon, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, ProdInra, Archive Ouverte, Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Universidade de Lisboa = University of Lisbon (ULISBOA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
[SDV] Life Sciences [q-bio], storage, blé, wheat, [SDV]Life Sciences [q-bio], stockage
Abstract

Proteomics has played an important role in advancing our understanding of abiotic stress response mechanisms in a wide range of crops by giving important insights into dynamic changes in protein abundance and translational and post-translational regulations. The C-termini of proteins undergo general and distinct chemical modifications that serve as recognition signatures in a variety of cellular processes, such as protein sorting, integration of proteins into membranes, regulation of protein activity, and formation of macromolecular complexes. These molecular mechanisms are partly genetically controlled but are also strongly influenced by environmental factors such as water availability and temperature, etc.In some fields there is renewed interest in analysing the C-termini of proteins in a proteome but this subfield remains largely unexplored in agronomy and crop science. Annotation of the carboxyl-terminal status of a proteome may facilitate functional classification, identification of novel proteoforms, and detection of unknown biochemical pathways. The use of C-terminal analysis has been limited so far mainly because of the technical challenge of dealing with the intrinsically low chemical reactivity of carboxyl groups. However, in recent years technological advances in mass spectrometry have made it possible to develop more efficient analytical techniques targeting C-termini.Studying the C-terminomics of plant abiotic stress responses could be a way to push forward this domain. C-terminal analysis techniques and principles developed in recent years will be reviewed and discussed here.

Published
2018

23. GlutNsafe : Vers la sélection de variétés de blés au gluten plus digestible?

Author

Ravel, Catherine, Bancel, Emmanuelle, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), ProdInra, Archive Ouverte, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Fonds de soutien à l'obtention végétale, and Bernard, Nathalie

Subjects
[SDV] Life Sciences [q-bio], variety, variété, blé, wheat, gluten, [SDV]Life Sciences [q-bio]
Abstract

International audience; A l'occasion de sa 8ème Rencontre scientifique, le FSOV propose "une journée entièrement consacrée à la restitution des résultats des 15 programmes de recherche financés dans le cadre de l’appel à propositions du FSOV 2016"

Published
2017

24. Recherche d’interactions protéines-protéines par une approche protéomique : Développement méthodologique

Author

Papon, Nathan, Bancel, Emmanuelle, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], approche protéomique
Abstract

Recherche d’interactions protéines-protéines par une approche protéomique : Développement méthodologique. 6. Colloque du Réseau Français de Biologie des Graines

Published
2017

25. Réponse du grain de blé à la nutrition azotée et soufrée : intégration de données « omiques »

Author

Bancel, Emmanuelle, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], nitrogen nutrition, [SDV]Life Sciences [q-bio], grain de blé, nutrition azotée, nutrition soufrée
Abstract

Réponse du grain de blé à la nutrition azotée et soufrée : intégration de données « omiques ». Journée Protéome vert

Published
2017

26. How einkorn (Triticum monoccocum) deals with variable nitrogen (N) and sulphur (S) supply during grain development? Exploration through proteome investigation

Author

Bancel, Emmanuelle, Bonnot, Titouan, Alvarez, David, Davanture, Marlène, Zivy, Michel, Martre, Pierre, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
azote, proteome, [SDV]Life Sciences [q-bio], food and beverages, sulphur s (symbol), grain de blé, protéome, nitrogen, soufre
Abstract

Book of abstracts, ISBN: 978-80-970662-3-9, EAN: 9788097066239 Poster p. 52Book of abstracts, ISBN: 978-80-970662-3-9, EAN: 9788097066239Poster p. 52; The concentration and composition of storage proteins in mature wheat grain and consequently its quality are strongly affected by nitrogen (N) and sulphur (S) supply1. Einkorn (Triticum monoccocum), a wheat diploid species was used as a model of bread wheat (T. aestivum). Einkorn plants were grown in a greenhouse under controlled conditions with different N and S amounts. Grains were harvested at different stages from 300 to 600°Cd corresponding to the filling phase of grain development. Proteomics approach by gel-free mass spectrometry analysis was used to study effects of N and S nutrition on metabolic proteins (albumins-globulins).The accumulation of storage proteins was affected by N and S supply, with significant changes in the grain composition at maturity. In particular, the accumulation of S-rich and S-poor storage proteins were differentially impacted by S supply. Some metabolic proteins (155 albumins-globulins) were also differentially accumulated in response to the nutrition. A number of proteins involved in key biological functions were either up- or down-regulated. We focus on the data set obtained in response to S supply in order to highlight some proteins and underline mechanisms occurring in response to nutrition in wheat grain.

Published
2016

27. Les problèmes de santé induits par les protéines du gluten

Author

Bancel, Emmanuelle, Branlard, Gerard, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Association Sélectionneurs Français., and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], protéine, gluten, [SDV]Life Sciences [q-bio], protein, problème de santé publique
Abstract

Les problèmes de santé induits par les protéines du gluten. Journée Association Sélectionneurs Français section Céréales à Paille, Débouchés alimentaires et non-alimentaires des Céréales

Published
2016

28. Integration of 'omics' data provides tools for understanding effects of variable nitrogen (N) and sulphur (S) supply during wheat grain development

Author

Bonnot, Titouan, Bancel, Emmanuelle, Alvarez, David, Dardevet, Mireille, Boudet, Julie, Davanture, Marlène, Moing, Annick, Gibon, Yves, Zivy, Michel, Pailloux, Marie, Martre, Pierre, Ravel, Catherine, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Laboratoire d'Informatique, de Modélisation et d'Optimisation des Systèmes (LIMOS), Ecole Nationale Supérieure des Mines de St Etienne (ENSM ST-ETIENNE)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Ecole Nationale Supérieure des Mines de St Etienne-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Ecole Nationale Supérieure des Mines de St Etienne-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), and Ecole Nationale Supérieure des Mines de St Etienne-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])

Subjects
azote, [SDV]Life Sciences [q-bio], sulphur s (symbol), grain de blé, nitrogen, soufre
Abstract

Integration of “omics” data provides tools for understanding effects of variable nitrogen (N) and sulphur (S) supply during wheat grain development. COST FA1306 meeting

Published
2016

29. Evidence of post-translational cleavage at the C-terminal domain of the 1By high molecular weight subunits of wheat glutenin (HMW-GS)

Author

Miranda, Julio, Bancel, Emmanuelle, Capelo, José L, Ravel, Catherine, Branlard, Gerard, Igrejas, Gilberto, ProdInra, Archive Ouverte, Function Genomic and Prote Unity, Univ Tras Os Montes and Alto Douro, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Faculty of Ciencias and Tecnology, Department of Quim, Universidade Nova de Lisboa (NOVA), and Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], gluténine, blé, [SDV]Life Sciences [q-bio], wheat, food and beverages, glutenin
Abstract

Evidence of post-translational cleavage at the C-terminal domain of the 1By high molecular weight subunits of wheat glutenin (HMW-GS). 5. Colloque du Réseau Français de Biologie des Graines

Published
2015

30. Extraction and Proteome Analysis of Starch Granule-Associated Proteins in Mature Wheat Kernel (Triticum aestivumL.)

Author

Bancel, Emmanuelle, Rogniaux, Hélène, Debiton, Clément, Chambon, Christophe, and Branlard, Gérard

Abstract

Starch consists of the two glucose polymers, amylose and amylopectin, and is deposited as semicrystalline granules inside plastids. The starch granule proteome is particularly challenging to study due to the amount of interfering compounds (sugars, storage proteins), the very low starch granule-associated protein content and also the dynamic range of abundant proteins. Here we present the protocol for extraction and 2-DE of wheat starch granule-associated proteins whose most important steps are: (i) washing and sonication to remove interfering compounds (storage proteins) from the surface of the granules, (ii) scanning electron microscopy (SEM) observations to monitor purification and granules swelling, (iii) appropriate protein extraction and solubilization to obtain enough proteins for Coomassie blue staining and proteomic analysis. Our objective was to minimize the amount of contamination by storage proteins and to preserve the structure of the starch and of starch-associated proteins and to maximize the number of polypeptides that can be resolved. For quantitative proteomic analysis of proteins associated with wheat starch granules, we developed a two-step protein extraction protocol including TCA/acetone precipitation and phenol extraction. With this protocol, proteins were extracted from wheat starch granules and solubilized and satisfactory blue-stained 2-DE protein maps were obtained. The majority of the spots associated with starch granules were identified by peptide mass fingerprinting and MS/MS and functionally classified into carbohydrate metabolism and stress defense.

Published
2024
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31. Identification and molecular characterization of oat peptides implicated on coeliac immune response

Author

Universidad de Sevilla. Departamento de Microbiología y Parasitología, Comino Montilla, Isabel María, Bernardo, David, Bancel, Emmanuelle, Moreno Amador, María de Lourdes, Sánchez, Borja, Barro, Francisco, Šuligoj, Tanja, Ciclitira, Paul Jonathan, Cebolla, Ángel, Knight, Stella Catherine, Branlard, Gérard, Sousa Martín, Carolina, Universidad de Sevilla. Departamento de Microbiología y Parasitología, Comino Montilla, Isabel María, Bernardo, David, Bancel, Emmanuelle, Moreno Amador, María de Lourdes, Sánchez, Borja, Barro, Francisco, Šuligoj, Tanja, Ciclitira, Paul Jonathan, Cebolla, Ángel, Knight, Stella Catherine, Branlard, Gérard, and Sousa Martín, Carolina

Abstract

Background : Oats provide important nutritional and pharmacological properties, although their safety in coeliac patients remains controversial. Previous studies have confirmed that the reactivity of the anti-33-mer monoclonal antibody with different oat varieties is proportional to the immune responses in terms of T-cell proliferation. Although the impact of these varieties on the adaptive response has been studied, the role of the dendritic cells (DC) is still poorly understood. The aim of this study is to characterize different oat fractions and to study their effect on DC from coeliac patients. Methods and results : Protein fractions were isolated from oat grains and analyzed by SDS PAGE. Several proteins were characterized in the prolamin fraction using immunological and proteomic tools, and by Nano- LC-MS/MS . These proteins, analogous to a - and g -gliadin-like, showed reactive sequences to anti-33-mer antibody suggesting their immunogenic potential. That was further confirmed as some of the newly identified oat peptides had a differential stimulatory capacity on circulating DC from coeliac patients compared with healthy controls. Conclusions : This is the first time, to our knowledge, where newly identified oat peptides have been shown to elicit a differential stimulatory capacity on circulating DC obtained from coeliac patients, potentially identifying immunogenic properties of these oat peptides

Published
2016

32. Identification and molecular characterization of oat peptides implicated on coeliac immune response

Author

Comino, Isabel, primary, Bernardo, David, additional, Bancel, Emmanuelle, additional, Moreno, María de Lourdes, additional, Sánchez, Borja, additional, Barro, Francisco, additional, Šuligoj, Tanja, additional, Ciclitira, Paul J., additional, Cebolla, Ángel, additional, Knight, Stella C., additional, Branlard, Gérard, additional, and Sousa, Carolina, additional

Published
2016
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33. Coping with wheat quality in a changing environment –Proteomics evidence for stress caused by environmental changes

Author

Lesage, Véronique S., Branlard, Gerard, Bancel, Emmanuelle, Martre, Pierre, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

34. Monitoring of storage proteins: accumulation and impact of high temperature in wheat kernel using proteomics

Author

Tasleem-Tahir, A., Bancel, Emmanuelle, Martre, P., Branlard, Gerard, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

37. Proteomics evidence of quality stresses caused by changing environment

Author

Branlard, Gerard, Bancel, Emmanuelle, Majoul, T., Martre, P., Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

38. Proteomics analysis of albumins and globulins from either endosperm or the aleurone layer

Author

Branlard, Gerard, Merlino, Marielle, Nadaud, Isabelle, Bancel, Emmanuelle, Laubin, Bastien, Unité mixte de recherche amélioration et santé des plantes, and Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)

Subjects
wheat endosperm, proteomics, chromosome mapping, [SDV]Life Sciences [q-bio], developing kernel, food and beverages
Abstract

To illustrate the usefulness of the proteomics tool, we report on two experiments whose objectives were to (1) analyse albumins and globulins in developing kernel, or (2) map albumins and globulins using segregating progeny, or reveal these proteins in the aleurone layer of two different cultivar. Proteomic analysis every 50°C day (average cumulative day temperature) during kernel formation from the ovule stage to 18 days after anthesis allowed many albumins and globulins to be tracked during cell and tissue formation. Quantitative analysis of spot volume and qualitative analysis (appearance/disappearance of a spot) evidenced five types of protein expression. Two-dimensional electrophoresis pH 4-7 x SDS Page of the albumins-globulins extracted from wholemeal kernels of ‘Synthetic’ or ‘Opata’ revealed 2125 and 2163 spots respectively. Only 10.6% of the spots differed between these two parents of the mapped progeny. The unbiased segregating spots were successfully mapped on 21 chromosomes. Wheat AL was isolated from mature seeds of cv. Chinese Spring and cv. Recital and more than 700 Coomassie stained proteins were revealed on 24 cm IPGE pH 3-10 x SDS-Page. Nearly 20% of these spots differed between the two cultivars. Proteomics analysis of seed is an increasingly available tool that enables biochemists and geneticists to investigate the diversity and function of the proteins expressed in different cell organites such as protein bodies, starch granules or kernel compartments

Published
2007

40. Analysis of the effect of heat shock on wheat storage protein

Author

Majoul, Thouraya, Bancel, Emmanuelle, Triboi, Eugeniu, Ben Hamida, Jeannette, Branlard, Gerard, ProdInra, Archive Ouverte, Faculté des Sciences de Tunis, Département de Biologie, Laboratoire de Biochimie des Protéines, Université de Tunis, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), UR 0874 Unité de recherche Agronomie, Institut National de la Recherche Agronomique (INRA)-Environnement et Agronomie (E.A.)-Ecologie des Forêts, Prairies et milieux Aquatiques (EFPA), Institut National de la Recherche Agronomique (INRA)-Unité de recherche Agronomie (UA), and Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

absent

Published
2003
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