462 results on '"Bang Yeon Hwang"'
Search Results
2. Targeted Isolation of N‑Acetylcysteine-Containing Angucycline Derivatives from Streptomyces sp. MC16 and Their Antiproliferative Effects
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Jun Gu Kim, Byeongsan Lee, Jae Sang Han, Taehoon Oh, Beomcheol Park, Yong Beom Cho, Beom Kyun An, Jin Won Choi, Sung-Kyun Ko, Mi Kyeong Lee, Young-Soo Hong, and Bang Yeon Hwang
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Chemistry ,QD1-999 - Published
- 2023
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3. Assessment of iridoid profiles in the growth period of aerial parts of Pseudolysimachion rotundum var. subintegrum and their antioxidant and MUC5AC inhibitory potential
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Soobin Song, Doo-Young Kim, Seon Min Oh, So-Yeun Woo, Il-joo Kim, Mun-Ock Kim, Ji-Yoon Park, Namho Kim, Hae-Young Kim, Juhee Lee, Sang Yoon Kim, Bang Yeon Hwang, Hyung Won Ryu, and Sei-Ryang Oh
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Pseudolysimachion rotundum var. Subintegrum ,Growth stages ,Quality control ,Antioxidant ,MUC5AC ,Agriculture (General) ,S1-972 ,Chemistry ,QD1-999 - Abstract
Abstract YPL-001 is a drug substance of Pseudolysimachion rotundum var. subintegrum and has been reported to be a potent COPD inhibitor. For the first time, this study demonstrated a correlation among the iridoid constituents, antioxidants, and MUC5AC inhibition activities in P. rotundum during different growth stages (5 to 11 weeks). Single-factor extraction was used to optimize the plant extraction conditions to maximize the major iridoid constituents (70% ethanol, 40 °C, 1 h); isolated metabolites 1–6 were identified using nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). The contents of each metabolite and antioxidant/MUC5AC inhibition effects were markedly changed according to the growth stages, especially for catalposide (2, 5.97 → 10.99 mg/g, 1.8-fold) and isovanillyl catapol (5, 4.42 → 20.00 mg/g, 4.5-fold), which were the predominant substances in August. Our results indicated that YPL-001 could potentially contribute to enhancing the P. rotundum value in accumulated iridoids at the growth stage and the biological effect aspects to develop industrial medicinal crops.
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- 2023
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4. A unique dual acyltransferase system shared in the polyketide chain initiation of kidamycinone and rubiflavinone biosynthesis
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Kyung Taek Heo, Byeongsan Lee, Gwi Ja Hwang, Beomcheol Park, Jun-Pil Jang, Bang Yeon Hwang, Jae-Hyuk Jang, and Young-Soo Hong
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biosynthesis ,natural products ,polyketide synthase ,dual acyltransferase ,Streptomyces ,Microbiology ,QR1-502 - Abstract
The pluramycin family of natural products has diverse substituents at the C2 position, which are closely related to their biological activity. Therefore, it is important to understand the biosynthesis of C2 substituents. In this study, we describe the biosynthesis of C2 moieties in Streptomyces sp. W2061, which produces kidamycin and rubiflavinone C-1, containing anthrapyran aglycones. Sequence analysis of the loading module (Kid13) of the PKS responsible for the synthesis of these anthrapyran aglycones is useful for confirming the incorporation of atypical primer units into the corresponding products. Kid13 is a ketosynthase-like decarboxylase (KSQ)-type loading module with unusual dual acyltransferase (AT) domains (AT1-1 and AT1-2). The AT1-2 domain primarily loads ethylmalonyl-CoA and malonyl-CoA for rubiflavinone and kidamycinone and rubiflavinone, respectively; however, the AT1-1 domain contributed to the functioning of the AT1-2 domain to efficiently load ethylmalonyl-CoA for rubiflavinone. We found that the dual AT system was involved in the production of kidamycinone, an aglycone of kidamycin, and rubiflavinone C-1 by other shared biosynthetic genes in Streptomyces sp. W2061. This study broadens our understanding of the incorporation of atypical primer units into polyketide products.
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- 2023
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5. Chemical inhibition of TRAF6-TAK1 axis as therapeutic strategy of endotoxin-induced liver disease
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Song-Hee Kim, Seung-Il Baek, Jihye Jung, Eung-Seok Lee, Younghwa Na, Bang Yeon Hwang, Yoon-Seok Roh, Jin Tae Hong, Sang-Bae Han, and Youngsoo Kim
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Bacterial endotoxin ,Hepatocyte apoptosis ,TNF-α ,TRAF6 ubiquitination ,TAK1 activation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The liver is exposed to gut-derived bacterial endotoxin via portal circulation, and recognizes it through toll-like receptor 4 (TLR4). Endotoxin lipopolysaccharide (LPS) stimulates the self-ubiquitination of ubiquitin ligase TRAF6, which is linked to scaffold with protein kinase TAK1 for auto-phosphorylation and subsequent activation. TAK1 activity is a signal transducer in the activating pathways of transcription factors NF-κB and AP-1 for production of various cytokines. Here, we hypothesized that TRAF6-TAK1 axis would be implicated in endotoxin-induced liver disease. Following exposure to endotoxin LPS, TLR4-mediated phosphorylation of TAK1 and transcription of cell-death cytokine TNF-α were triggered in Kupffer cells but not in hepatocytes as well as TNF receptor-mediated and caspase-3-executed apoptosis was occurred in D-galactosamine (GalN)-sensitized hepatocytes under co-culture with Kupffer cells. Treatment with pyridinylmethylene benzothiophene (PMBT) improved endotoxin LPS-induced hepatocyte apoptosis in GalN-sensitized C57BL/6 mice via suppressing NF-κB- and AP-1-regulated expression of TNF-α in Kupffer cells, and rescued the mice from hepatic damage-associated bleeding and death. As a mechanism, PMBT directly inhibited Lys 63-linked ubiquitination of TRAF6, and mitigated scaffold assembly between TRAF6 and the TAK1-activator adaptors TAB1 and TAB2 complex in Kupffer cells. Thereby, PMBT interrupted TRAF6 ubiquitination-induced activation of TAK1 activity in the TLR4-mediated signal cascade leading to TNF-α production. However, PMBT did not directly affect the apoptotic activity of TNF-α on GalN-sensitized hepatocytes. Finally, we propose chemical inhibition of TRAF6-TAK1 axis in Kupffer cells as a strategy for treating liver disease due to gut-derived endotoxin or Gram-negative bacterial infection.
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- 2022
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6. Cordyceps mushroom with increased cordycepin content by the cultivation on edible insects
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Ayman Turk, Mohamed A. A. Abdelhamid, Sang Won Yeon, Se Hwan Ryu, Solip Lee, Sung Min Ko, Beom Seok Kim, Seung Pil Pack, Bang Yeon Hwang, and Mi Kyeong Lee
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Cordyceps militaris ,cordycepin ,Allomyrina dichotoma ,oleic acid ,cns1 and cns2 ,Microbiology ,QR1-502 - Abstract
Cordycepin is the major constituent of Cordyceps mushroom (or Cordyceps militaris) with therapeutic potential. Insects are the direct sources of nutrients for Cordyceps in nature. Therefore, optimized condition of Cordyceps cultivation for efficient cordycepin production was explored using six edible insects as substrates. The highest yield of cordycepin was produced by the cultivation on Allomyrina dichotoma and was 34 times that on Bombyx mori pupae. Among insect components, fat content was found to be important for cordycepin production. Especially, a positive correlation was deduced between oleic acid content and cordycepin production. The transcriptional levels of cns1 and cns2, genes involved in cordycepin biosynthesis, were higher in Cordyceps grown on A. dichotoma than on other insects tested. The addition of oleic acid to the substrates increased cordycepin production together with the transcriptional levels of cns1 and cns2. Therefore, Cordyceps with high content of cordycepin can be secured by the cultivation on insects.
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- 2022
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7. Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
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Ayman Turk, Solip Lee, Sang Won Yeon, Se Hwan Ryu, Yoo Kyong Han, Young Jun Kim, Sung Min Ko, Beom Seok Kim, Bang Yeon Hwang, Ki Yong Lee, and Mi Kyeong Lee
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Cordyceps militaris ,Mori Folium ,adenosine deaminase (ADA) ,cordycepin ,medicinal plants ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of Cordyceps militaris with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of Cordyceps to increase the cordycepin content. Cordyceps cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3′-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in C. militaris.
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- 2023
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8. Curcubinoyl flavonoids from wild ginseng adventitious root cultures
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Qing Liu, Seon Beom Kim, Yang Hee Jo, Jong Hoon Ahn, Ayman Turk, Da Eun Kim, Bo Yoon Chang, Sung Yeon Kim, Cheol-Seung Jeong, Bang Yeon Hwang, So-Young Park, and Mi Kyeong Lee
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Medicine ,Science - Abstract
Abstract Wild ginseng (Panax ginseng) adventitious root cultures were prepared by elicitation using methyl jasmonate and investigated further to find new secondary metabolites. Chromatographic fractionation of wild ginseng adventitious root cultures led to the isolation of eleven compounds. The chemical structures of isolated compounds were identified as four known flavanone derivatives (1–4), one new curcubinoyl derivative, jasmogin A (5) and six new curcubinoyl-flavanone conjugates, jasmoflagins A-F (6–11) by extensive spectroscopic analysis. Newly isolated curcubinoyl derivatives showed inhibitory activity against lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages. Therefore, our present study suggested that elicitor stimulated plant cell cultures might contribute to the production of new metabolites.
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- 2021
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9. Characterization of Antioxidant and α-Glucosidase Inhibitory Compounds of Cratoxylum formosum ssp. pruniflorum and Optimization of Extraction Condition
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Heewon An, Le Nguyen Thanh, Le Quoc Khanh, Se Hwan Ryu, Solip Lee, Sang Won Yeon, Hak Hyun Lee, Ayman Turk, Ki Yong Lee, Bang Yeon Hwang, and Mi Kyeong Lee
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Cratoxylum formosum ssp. pruniflorum ,xanthone ,benzophenone ,antioxidant ,α-glucosidase ,response surface methodology ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Cratoxylum formosum ssp. pruniflorum (Kurz.) Gogel (Guttiferae), called kuding tea, is widely distributed in Southeast Asia. In this study, the constituents and biological activity of C. formosum ssp. pruniflorum were investigated. Extract of its leaves, roots and stems showed antioxidant and α-glucosidase inhibitory activity. Interestingly, comparison of the metabolite profiles of leaves, roots and stems of C. formosum ssp. pruniflorum by LC-MS analysis showed a great difference between the roots and leaves, whereas the roots and stems were quite similar. Purification of the roots and leaves of C. formosum ssp. pruniflorum through various chromatographic techniques resulted in the isolation of 25 compounds. The structures of isolated compounds were elucidated on the basis of spectroscopic analysis as 18 xanthones, 5 flavonoids, a benzophenone and a phenolic compound. Among them, a xanthone (16) and a benzophenone (19) were first reported from nature. Evaluation of biological activity revealed that xanthones had a potent α-glucosidase inhibitory activity, while flavonoids were responsible for the antioxidant activity. To maximize the biological activity, yield and total phenolic content of C. formosum ssp. pruniflorum, extraction conditions such as extraction solvent, time and temperature were optimized using response surface methodology with Box–Behnken Design (BBD). Regression analysis showed a good fit of the experimental data, and the optimal condition was obtained as MeOH concentration in EtOAc, 88.1%; extraction time, 6.02 h; and extraction temperature 60.0 °C. α-Glucosidase inhibitory activity, yield and total phenolic content under the optimal condition were found to be 72.2% inhibition, 10.3% and 163.9 mg GAE/g extract, respectively. These results provide useful information about C. formosum ssp. pruniflorum as functional foods for oxidative stress–related metabolic diseases.
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- 2023
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10. Ethanol extract from Gynostemma pentaphyllum ameliorates dopaminergic neuronal cell death in transgenic mice expressing mutant A53T human alpha-synuclein
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Hyun Jin Park, Ting Ting Zhao, Seung Hwan Kim, Chong Kil Lee, Bang Yeon Hwang, Kyung Eun Lee, and Myung Koo Lee
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A53T α-synuclein genetic mice ,ERK1/2 ,gynostemma pentaphyllum ,gypenosides ,Parkinson’s disease ,retention transfer latency time ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Gynostemma (G.) pentaphyllum (Cucurbitaceae) contains various bioactive gypenosides. Ethanol extract from G. pentaphyllum (GP-EX) has been shown to have ameliorative effects on the death of dopaminergic neurons in animal models of Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- and 6-hydroxydopamine. PD patients exhibit multiple symptoms, so PD-related research should combine neurotoxin models with genetic models. In the present study, we investigated the ameliorative effects of GP-EX, including gypenosides, on the cell death of dopaminergic neurons in the midbrain of A53T α-synuclein transgenic mouse models of PD (A53T). Both GP-EX and gypenosides at 50 mg/kg per day were orally administered to the A53T mice for 20 weeks. α-Synuclein-immunopositive cells and α-synuclein phosphorylation were increased in the midbrain of A53T mice, which was reduced following treatment with GP-EX. Treatment with GP-EX modulated the reduced phosphorylation of tyrosine hydroxylase, extracellular signal-regulated kinase (ERK1/2), Bcl-2-associated death promoter (Bad) at Ser112, and c-Jun N-terminal kinase (JNK1/2) due to α-synuclein overexpression. In the A53T group, GP-EX treatment prolonged the latency of the step-through passive avoidance test and shortened the transfer latency of the elevated plus maze test. Gypenosides treatment exhibited the effects and efficacy similar to those of GP-EX. Taken together, GP-EX, including gypenosides, has ameliorative effects on dopaminergic neuronal cell death due to the overexpression of α-synuclein by modulating ERK1/2, Bad at Ser112, and JNK1/2 signaling in the midbrain of A53T mouse model of PD. Further studies are needed to investigate GP-EX as a treatment for neurodegenerative synucleinopathies, including PD. This study was approved by the Animal Ethics Committee of Chungbuk National University (approval No. CBNUA-956-16-01) on September 21, 2016.
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- 2020
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11. A Natural CHI3L1—Targeting Compound, Ebractenoid F, Inhibits Lung Cancer Cell Growth and Migration and Induces Apoptosis by Blocking CHI3L1/AKT Signals
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Da Eun Hong, Ji Eun Yu, Jin Woo Lee, Dong Ju Son, Hee Pom Lee, Yuri Kim, Ju Young Chang, Dong Won Lee, Won Kyu Lee, Jaesuk Yun, Sang Bae Han, Bang Yeon Hwang, and Jin Tae Hong
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ebractenoid F ,CHI3L1 ,AKT ,lung cancer cell growth inhibition ,Organic chemistry ,QD241-441 - Abstract
Our previous big data analyses reported a strong association between CHI3L1 expression and lung tumor development. In this present study, we investigated whether a CHI3L1-inhibiting natural compound, ebractenoid F, inhibits lung cancer cell growth and migration and induces apoptosis. Ebractenoid F concentration-dependently (0, 17, 35, 70 µM) and significantly inhibited the proliferation and migration of A549 and H460 lung cancer cells and induced apoptosis. In the mechanism study, we found that ebractenoid F bound to CHI3L1 and suppressed CHI3L1-associated AKT signaling. Combined treatment with an AKT inhibitor, LY294002, and ebractenoid F synergistically decreased the expression of CHI3L1. Moreover, the combination treatment further inhibited the growth and migration of lung cancer cells and further induced apoptosis, as well as the expression levels of apoptosis-related proteins. Thus, our data demonstrate that ebractenoid F may serve as a potential anti-lung cancer compound targeting CHI3L1-associated AKT signaling.
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- 2022
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12. Novel C-17 spirost protostane-type triterpenoids from Alisma plantago-aquatica with anti-inflammatory activity in Caco-2 cells
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Qinghao Jin, Jianqing Zhang, Jinjun Hou, Min Lei, Chen Liu, Xia Wang, Yong Huang, Shuai Yao, Bang Yeon Hwang, Wanying Wu, and Dean Guo
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Twenty-one protostane-type triterpenoids with diverse structures, including nine new compounds (1–9), were isolated from the of Alisma plantago-aquatica Linn. Structurally, alisolides A‒F (1–6), composed of an oxole group coupled to a five-membered ring, represent unusual C-17 spirost protostane-type triterpenoids. Alisolide H (8) is a novel triterpenoid with an unreported endoperoxide bridge. Alisolide I (9) represents the first example of 23,24-acetal triterpenoid. Their structures were elucidated based on spectroscopic analysis, wherein the absolute configurations of 4‒6, 8 were further confirmed by the Mo2(OAc)4-induced ECD method. Furthermore, all isolates were evaluated for their inhibitory effects on LPS-induced NO production in Caco-2 cells, and all the compounds showed remarkable inhibitory activities, with IC50 values in the range of 0.76–38.20 μmol/L. Key words: Alisma plantago-aquatica Linn., Protostane-type triterpenoids, Caco-2 cells, LPS-induced NO production
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- 2019
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13. Therapeutic Potentials of Secoiridoids from the Fruits of Ligustrum lucidum Aiton against Inflammation-Related Skin Diseases
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Sang Won Yeon, Su Ryeon Choi, Qing Liu, Yang Hee Jo, Da Hee Choi, Mi Ran Kim, Se Hwan Ryu, Solip Lee, Bang Yeon Hwang, Hyung Seo Hwang, and Mi Kyeong Lee
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Ligustrum lucidum ,secoligulene ,anti-inflammation ,iridoid ,psoriasis model ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
Ligustrum lucidum Aiton is a flowering plant of the Oleaceae family, and its fruits have been traditionally used for skin nourishment and the treatment of skin diseases. However, the anti-inflammatory constituents for skin disease are not well-characterized. Phytochemical investigation of L. lucidum fruits resulted in the isolation of a new secoiridoid, secoligulene (1), together with (E)-3-(1-oxobut-2-en-2-yl)pentanedioic acid (2) and trans-(E)-3-(1-oxobut-2-en-2-yl)glutaric acid (3). Secoligulene (1) displayed the potent inhibitory effect on NO production with an IC50 value of 12.0 μg/mL. Secoligulene (1) also downregulated mRNA transcriptional levels of pro-inflammatory cytokines such as IL-1 α, IL-1β, IL-6 and COX-2 in LPS-stimulated RAW264.7 cells. Further investigation showed that secoligulene (1) inhibited the phosphorylation of IκB and JNK activated by LPS. In addition, secoligulene (1) downregulated the expression of chemokines such as CXCL8 and CCL20 in the TNF-α/IL-17/IFN-γ induced HaCaT psoriasis model. Taken together, these findings support the beneficial effects of L. lucidum and its constituents on inflammation-related skin diseases and can be further developed as therapeutic treatments for related diseases.
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- 2022
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14. Dianthiamides A–E, Proline-Containing Orbitides from Dianthus chinensis
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Jin Woo Lee, Jun Gu Kim, Jae Sang Han, Yong Beom Cho, Yu Jin Lee, Dongho Lee, Dae Hwan Shin, Jin Tae Hong, Mi Kyeong Lee, and Bang Yeon Hwang
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Dianthus chinensis ,Caryophyllaceae ,orbitide ,structure elucidation ,cytotoxic activity ,Organic chemistry ,QD241-441 - Abstract
Orbitides are plant-derived small cyclic peptides with a wide range of biological activities. Phytochemical investigation of the whole plants of Dianthus chinensis was performed with the aim to discover new bioactive orbitides. Five undescribed proline-containing orbitides, dianthiamides A–E (1–5), were isolated from a methanolic extract of Dianthus chinensis. Their structures were elucidated by extensive analysis of 1D and 2D NMR and HRESI–TOF–MS as well as ESI–MS/MS fragmentation data. The absolute configuration of the amino acid residues of compounds 1–5 was determined by Marfey’s method. All compounds were tested for their cytotoxic activity, and dianthiamide A (1) exhibited weak activity against A549 cell line with IC50 value of 47.9 μM.
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- 2021
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15. Aromatic Constituents from the Leaves of Actinidia arguta with Antioxidant and α-Glucosidase Inhibitory Activity
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Jong Hoon Ahn, Se Hwan Ryu, Solip Lee, Sang Won Yeon, Ayman Turk, Yoo Kyong Han, Ki Yong Lee, Bang Yeon Hwang, and Mi Kyeong Lee
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Actinidia arguta ,aromatic ,argutosides A–E ,antioxidant ,α-glucosidase ,molecular docking analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
As the leaf of Actinidia arguta has shown antioxidant activity, a study was conducted to identify the active ingredients. Forty-eight compounds were isolated from the leaves of A. arguta through various chromatographic techniques. Further characterization of the structures on the basis of 1D and 2D NMR and MS data identified several aromatic compounds, including phenylpropanoid derivatives, phenolics, coumarins, flavonoids and lignans. Among them, five compounds were newly reported, naturally occurring, and named argutosides A–D (1–4), which consist of phenylpropanoid glycosides that are conjugated with a phenolic moiety, and argutoside E (5), which is a coumarin glycoside that is conjugated with a phenylpropanoid unit. The isolated compounds showed good antioxidant and α-glucosidase inhibitory activity with differences in activity depending on the structures. Molecular docking analysis demonstrated the interaction between the hydroxyl and carbonyl groups of compounds 1 and 5 with α-glucosidase. Taken together, the leaves of A. arguta are rich in aromatic compounds with diverse structures. Therefore, the leaves of A. arguta and their aromatic components might be beneficial for oxidative stress and glucose-related diseases.
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- 2021
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16. Effect of Korean Red Ginseng extraction conditions on antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content: optimization using response surface methodology
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Jin Woo Lee, Eun Jin Mo, Ji Eun Choi, Yang Hee Jo, Hari Jang, Ji Yeon Jeong, Qinghao Jin, Hee Nam Chung, Bang Yeon Hwang, and Mi Kyeong Lee
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antioxidant activity ,extraction conditions ,extraction yield ,optimization ,Panax ginseng (Korean Red Ginseng) ,Botany ,QK1-989 - Abstract
Background: Extraction conditions greatly affect composition, as well as biological activity. Therefore, optimization is essential for maximum efficacy. Methods: Korean Red Ginseng (KRG) was extracted under different conditions and antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content evaluated. Optimized extraction conditions were suggested using response surface methodology for maximum antioxidant activity and extraction yield. Results: Analysis of KRG extraction conditions using response surface methodology showed a good fit of experimental data as demonstrated by regression analysis. Among extraction factors, such as extraction solvent and extraction time and temperature, ethanol concentration greatly affected antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content. The optimal conditions for maximum antioxidant activity and extraction yield were an ethanol concentration of 48.8%, an extraction time 73.3 min, and an extraction temperature of 90°C. The antioxidant activity and extraction yield under optimal conditions were 43.7% and 23.2% of dried KRG, respectively. Conclusion: Ethanol concentration is an important extraction factor for KRG antioxidant activity and extraction yield. Optimized extraction conditions provide useful economic advantages in KRG development for functional products.
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- 2016
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17. Anti-Obesity Effect of 6,8-Diprenylgenistein, an Isoflavonoid of Cudrania tricuspidata Fruits in High-Fat Diet-Induced Obese Mice
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Yang Hee Jo, Kyeong-Mi Choi, Qing Liu, Seon Beom Kim, Hyeong-Jin Ji, Myounghwan Kim, Sang-Kyung Shin, Seon-Gil Do, Eunju Shin, Gayoung Jung, Hwan-Soo Yoo, Bang Yeon Hwang, and Mi Kyeong Lee
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6,8-Diprenylgenistein (DPG) ,Cudrania tricuspidata ,high-fat diet-induced obesity ,lipid profile ,AMPK ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Obesity, which is characterized by excessive fat accumulation, is associated with several pathological disorders, including metabolic diseases. In this study, the anti-obesity effect of 6,8-diprenylgenistein (DPG), a major isoflavonoid of Cudrania tricuspidata fruits was investigated using high fat-diet (HFD)-induced obese mice at the doses of 10 and 30 mg/kg for six week. The body weight of the DPG-treated groups was significantly lower compared to the HFD-treated group. In addition, fat accumulation in epididymal adipose tissue and liver was dramatically decreased in the HFD + DPG groups. The food efficiency ratios of the HFD + DPG groups were also lower compared to the HFD group with the same food intake. Metabolic parameters that had increased in the HFD group were decreased in the HFD + DPG groups. Further studies demonstrate that DPG efficiently reduces lipogenic genes by regulation of transcription factors, such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and hormones, such as leptin and adiponection. DPG also regulates acetyl-CoA carboxylase (ACC) and hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) by AMP-activated protein kinase (AMPK) activation. Taken together, DPG is beneficial for the regulation of obesity, especially resulting from high fat intake.
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- 2015
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18. Optimization of Extraction Condition of Bee Pollen Using Response Surface Methodology: Correlation between Anti-Melanogenesis, Antioxidant Activity, and Phenolic Content
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Seon Beom Kim, Yang Hee Jo, Qing Liu, Jong Hoon Ahn, In Pyo Hong, Sang Mi Han, Bang Yeon Hwang, and Mi Kyeong Lee
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bee pollen ,oxidative stress ,melanogenesis ,optimization ,phenolic content ,response surface methodology ,Organic chemistry ,QD241-441 - Abstract
Bee pollen is flower pollen with nectar and salivary substances of bees and rich in essential components. Bee pollen showed antioxidant and tyrosinase inhibitory activity in our assay system. To maximize the antioxidant and tyrosinase inhibitory activity of bee pollen, extraction conditions, such as extraction solvent, extraction time, and extraction temperature, were optimized using response surface methodology. Regression analysis showed a good fit of this model and yielded the second-order polynomial regression for tyrosinase inhibition and antioxidant activity. Among the extraction variables, extraction solvent greatly affected the activity. The optimal condition was determined as EtOAc concentration in MeOH, 69.6%; temperature, 10.0 °C; and extraction time, 24.2 h, and the tyrosinase inhibitory and antioxidant activity under optimal condition were found to be 57.9% and 49.3%, respectively. Further analysis showed the close correlation between activities and phenolic content, which suggested phenolic compounds are active constituents of bee pollen for tyrosinase inhibition and antioxidant activity. Taken together, these results provide useful information about bee pollen as cosmetic therapeutics to reduce oxidative stress and hyperpigmentation.
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- 2015
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19. Comparison of antibacterial activity and phenolic constituents of bark, lignum, leaves and fruit of Rhus verniciflua.
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Jae Young Jang, Hyeji Shin, Jae-Woong Lim, Jong Hoon Ahn, Yang Hee Jo, Ki Yong Lee, Bang Yeon Hwang, Sung-Ju Jung, So Young Kang, and Mi Kyeong Lee
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Medicine ,Science - Abstract
Rhus verniciflua is commonly known as a lacquer tree in Korea. The bark of R. verniciflua has been used as an immunostimulator in traditional medicine, but also causes allergic dermatitis due to urushiol derivatives. For the development of active natural resources with less toxicity, the antibacterial activity of various parts of R. verniciflua such as bark, lignum, leaves and fruit, together with chemical composition, were investigated. Among the various parts of R. verniciflua, lignum showed the most potent antibacterial activity against fish pathogenic bacteria such as Edwardsiella tarda, Vibrio anguillarum and Streptococcus iniae. Measurement of total phenolic content and flavonoid content clearly showed a high content of phenolic and flavonoids in lignum among the various parts of R. verniciflua. Further analysis showed a close correlation between antibacterial activity and phenolic content. In addition, methyl gallate and fustin, the major constituents of bark and lignum, showed antibacterial activity, which suggested phenolic constituents as active constituents. The content of urushiols, however, was highest in bark, but there was a trace amount in lignum. LC-MS-MS and PCA analysis showed good discrimination with the difference of phenolic composition in various parts of R. verniciflua. Taken together, phenolic compounds are responsible for the antibacterial activity of R. verniciflua. The lignum of R. verniciflua contains high content of phenolic compounds with less urushiols, which suggests efficient antibacterial activity with less toxicity. Therefore, the lignum of R. verniciflua is suggested as a good source for antibacterial material to use against fish bacterial diseases.
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- 2018
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20. Synthesis and Biological Evaluation of Resveratrol Derivatives as Melanogenesis Inhibitors
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Qing Liu, CheongTaek Kim, Yang Hee Jo, Seon Beom Kim, Bang Yeon Hwang, and Mi Kyeong Lee
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melanin ,resveratrol derivatives ,tyrosinase ,tyrosinase-related protein (TRP)-1 ,Organic chemistry ,QD241-441 - Abstract
Resveratrol (1), a naturally occurring stilbene compound, has been suggested as a potential whitening agent with strong inhibitory activity on melanin synthesis. However, the use of resveratrol in cosmetics has been limited due to its chemical instability and poor bioavailability. Therefore, resveratrol derivatives were prepared to improve bioavailability and anti-melanogenesis activity. Nine resveratrol derivatives including five alkyl ether derivatives with C2H5, C4H9, C5H11, C6H13, and C8H17 (2a–2e) and four ester derivatives with CH3, CH=C(CH3)2, CH(C2H5)C4H9, C7H15 (3a–3d) were newly synthesized and their effect on melanin synthesis were assessed. All the synthetic derivatives efficiently reduced the melanin content in α-MSH stimulated B16F10 melanoma cells. Further investigation showed that the inhibitory effect of 2a on melanin synthesis was achieved not by the inhibition of tyrosinase activity but by the inhibition of melanogenic enzyme expressions such as tyrosinase and tyrosinase-related protein (TRP)-1. Our synthetic resveratrol derivatives have more lipophilic properties than resveratrol by the addition of alkyl or acyl chains to free hydroxyl moiety of resveratrol; thus, they are expected to show better bioavailability in skin application. Therefore, we suggest that our synthetic resveratrol derivatives might be promising candidates for better practical application to skin-whitening cosmetics.
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- 2015
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21. Characterization of Melanogenesis Inhibitory Constituents of Morus alba Leaves and Optimization of Extraction Conditions Using Response Surface Methodology
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Ji Yeon Jeong, Qing Liu, Seon Beom Kim, Yang Hee Jo, Eun Jin Mo, Hyo Hee Yang, Dae Hye Song, Bang Yeon Hwang, and Mi Kyeong Lee
- Subjects
Morus alba ,melanogenesis ,optimization ,tyrosinase ,melanin ,phenolic content ,Organic chemistry ,QD241-441 - Abstract
Melanin is a natural pigment that plays an important role in the protection of skin, however, hyperpigmentation cause by excessive levels of melatonin is associated with several problems. Therefore, melanogenesis inhibitory natural products have been developed by the cosmetic industry as skin medications. The leaves of Morus alba (Moraceae) have been reported to inhibit melanogenesis, therefore, characterization of the melanogenesis inhibitory constituents of M. alba leaves was attempted in this study. Twenty compounds including eight benzofurans, 10 flavonoids, one stilbenoid and one chalcone were isolated from M. alba leaves and these phenolic constituents were shown to significantly inhibit tyrosinase activity and melanin content in B6F10 melanoma cells. To maximize the melanogenesis inhibitory activity and active phenolic contents, optimized M. alba leave extraction conditions were predicted using response surface methodology as a methanol concentration of 85.2%; an extraction temperature of 53.2 °C and an extraction time of 2 h. The tyrosinase inhibition and total phenolic content under optimal conditions were found to be 74.8% inhibition and 24.8 μg GAE/mg extract, which were well-matched with the predicted values of 75.0% inhibition and 23.8 μg GAE/mg extract. These results shall provide useful information about melanogenesis inhibitory constituents and optimized extracts from M. alba leaves as cosmetic therapeutics to reduce skin hyperpigmentation.
- Published
- 2015
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22. Comparison of pancreatic lipase inhibitory isoflavonoids from unripe and ripe fruits of Cudrania tricuspidata.
- Author
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Yang Hee Jo, Seon Beom Kim, Qing Liu, Seon-Gil Do, Bang Yeon Hwang, and Mi Kyeong Lee
- Subjects
Medicine ,Science - Abstract
The composition and content of the active constituents and their biological activity vary according to diverse factors including their maturation stages. A previous study showed that the fruits of Cudrania tricuspidata inhibited pancreatic lipase activity, a key enzyme in fat absorption. In this study, we investigated the chemical composition and pancreatic lipase inhibitory activity of unripe and ripe fruits of C. tricuspidata. Unripe fruits of C. tricuspidata have a higher content of total phenolic and flavonoids and exhibited stronger pancreatic lipase inhibition compared to ripe fruits. HPLC analysis revealed the different chemical compositions of the unripe and ripe fruits. Further fractionation resulted in the isolation of 30 compounds including two new isoflavonoids. Analysis of the chemical constituents of the unripe and ripe fruits revealed that a 2,2-dimethylpyran ring, a cyclized prenyl, was the predominant side chain in the unripe fruits, whereas it was a linear prenyl group in the ripe fruits. In addition, a new isoflavonoid (19) from the unripe fruits showed the most potent inhibition on pancreatic lipase. Taken together, the maturation stage is an important factor for maximum efficacy and that unripe fruits of C. tricuspidata are a good source of new bioactive constituents for the regulation of obesity.
- Published
- 2017
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23. Antiplatelet and antithrombotic effect of Phyllostachys pubescens leaves and Mume Fructus combination
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Wen Yi Jin, Seung-Hyung Kim, Ho Kyoung Kim, Dong Gyu Jang, Jeong Bum Nam, Young Min Kang, Bang Yeon Hwang, and Dong-Seon Kim
- Subjects
antiplatelet ,antithrombosis ,blood circulation ,herbal combination ,Mume Fructus ,Phyllostachys pubescens ,Miscellaneous systems and treatments ,RZ409.7-999 - Abstract
Background: Agents currently used for the treatment and prevention of thrombosis have a number of side effects. We conducted this study to develop antithrombotic agents from herbs that are used in food. Methods: The 80% (v/v) ethanol extracts of Phyllostachys pubescens leaf (PL) and Mume Fructus (MF) and their combinations—2:1 (PM21), 1:1 (PM11), and 1:2 (PM12)—were evaluated on rat platelet aggregation induced by adenosine diphosphate (ADP) in vitro and on arteriovenous shunt thrombosis after 3 days of oral treatment in rats in vivo. Results: At 100 μg/mL, PM21 and PM11 inhibited in vitro ADP-induced aggregation by 44.0 ± 4.3% and 30.0 ± 3.2%, respectively, whereas PL, MF, and PM12 weakly or scarcely inhibited ADP-induced aggregation by 3.9 ± 3.2%, 13.0 ± 2.7%, and 5.2 ± 1.3%, respectively. The IC50 values of PM21 on ADP-, collagen-, and thrombin-induced platelet aggregations were 135.6 ± 7.4 μg/mL, 142.7 ± 5.8 μg/mL, and 186.5 ± 9.7 μg/mL, respectively. In an in vivo rat arteriovenous-shunt thrombosis model, thrombus weight was significantly decreased after the oral administration of 400 mg/kg PL (27.8 ± 3.0%, p
- Published
- 2013
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24. Anxiolytic Effects of Herbal Ethanol Extract from Gynostemma pentaphyllum in Mice after Exposure to Chronic Stress
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Myung Koo Lee, Chong Kil Lee, Bang Yeon Hwang, Seung Hwan Kim, Keon Sung Shin, Ting Ting Zhao, and Hyun Sook Choi
- Subjects
Gynostemma pentaphyllum ,chronic stress ,anxiety disorders ,elevated plus-maze ,marble burying ,dopamine ,serotonin ,corticosterone ,c-Fos ,Organic chemistry ,QD241-441 - Abstract
In this study, the effects of herbal ethanol extracts of Gynostemma pentaphyllum (GP-EX), on chronic electric footshock (EF) stress-induced anxiety disorders were investigated in mice, which were orally treated with GP-EX (30 mg/kg and 50 mg/kg) once a day for 14 days, followed by exposure to EF stress (2 mA, with an interval and duration of 10 s for 3 min). After the final exposure to EF stress, the elevated plus-maze and marble burying tests were performed, and the levels of dopamine and serotonin in the brain, the serum levels of corticosterone, and the expression of c-Fos in the paraventricular nuclei (PVN) were determined. Treatment with GP-EX (30 mg/kg and 50 mg/kg) significantly recovered the number of entries into open arms and time spent on open arms, which was reduced by chronic EF stress. GP-EX (30 mg/kg and 50 mg/kg) also reduced the number of marbles buried, which was increased by chronic EF stress. In addition, electric EF stress significantly decreased the levels of dopamine and serotonin in the brain, which was recovered by treatment with GP-EX (30 mg/kg and 50 mg/kg). The serum levels of corticosterone, which were markedly increased by chronic EF stress, were reduced by treatment with GP-EX (30 mg/kg and 50 mg/kg). Chronic EF stress-induced increases in c-Fos expression were also markedly reduced by GP-EX (30 mg/kg and 50 mg/kg) in the PVN. These results suggest that GP-EX shows anxiolytic functions, determined by the elevated plus-maze and marble burying tests, which are mediated by modulating the activity of dopamine and serotonin neurons as well as the expression of c-Fos in the brain, and the serum levels of corticosterone. Clinical trials of herbal GP-EX and its bioactive components need further investigation.
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- 2013
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25. Restoration of Electric Footshock-Induced Immunosuppression in Mice by Gynostemma pentaphyllum Components
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Sun-A Im, Hyun Sook Choi, Soon Ok Choi, Ki-Hyang Kim, Seungjeong Lee, Bang Yeon Hwang, Myung Koo Lee, and Chong Kil Lee
- Subjects
Gynostemma pentaphyllum ,electric footshock ,stress ,immunomodulation ,cytotoxic T lymphocytes ,Organic chemistry ,QD241-441 - Abstract
The immunomodulatory effects of the ethanol extract of Gynostemma pentaphyllum (GP-EX) were examined in electric footshock (EFS)-stressed mice. The mice were orally administered various doses of GP-EX for 7 days before exposure to EFS (duration: 3 min, interval: 10 s, intensity: 2 mA) once a day from day 8 for 14 days with continuous daily feeding of GP-EX. Oral administration of GP-EX to mice prevented EFS stress-induced immunosuppression as determined by the lymphoid organ (thymus and spleen) weight and cellularity. In addition, oral administration of GP-EX restored EFS-suppressed functional properties of mature lymphocytes in terms of concanavalin A-induced proliferation of splenocytes and lipopolysaccharide-induced cytokine production (TNF-α, IL-1β). Furthermore, we found that mice that were orally administered with GP-EX generated much more potent ovalbumin-specific cytotoxic T lymphocyte responses upon intravenous ovalbumin injection compared to the untreated controls. These results demonstrate that oral administration of the ethanol extract of Gynostemma pentaphyllum could increase host defense in immunocompromised situations such as stress-induced immunosuppression.
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- 2012
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26. Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease
- Author
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Hyun Sook Choi, Mi Sook Park, Seung Hwan Kim, Bang Yeon Hwang, Chong Kil Lee, and Myung Koo Lee
- Subjects
Gynostemma pentaphyllum ,6-hydroxydopamine-lesioned rats ,tyrosine hydroxylase ,dopamine ,Parkinson’s disease ,Organic chemistry ,QD241-441 - Abstract
6-Hydroxydopamine administration for 28 days (8 μg/2 μL) reduced the number of tyrosine hydroxylase (TH)-immunopositive neurons to 40.2% in the substantia nigra compared to the intact contralateral side. Dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and norepinephrine levels were reduced to 19.1%, 52.3%, 47.1% and 67.4% in the striatum of 6-hydroxydopamine-lesioned rats compared to the control group, respectively. However, an oral administration of herbal ethanol extracts from Gynostemma pentaphyllum (GP-EX) (10 mg/kg and 30 mg/kg) starting on day 3 post-lesion for 28 days markedly ameliorated the reduction of TH-immunopositive neurons induced by 6-hydroxydopamine-lesioned rat brain from 40.2% to 67.4% and 75.8% in the substantia nigra. GP-EX administration (10 and 30 mg/kg) also recovered the levels of dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and norepinephrine in post-lesion striatum to 64.1% and 65.0%, 77.9% and 89.7%, 82.6% and 90.2%, and 88.1% and 89.2% of the control group. GP-EX at the given doses did not produce any sign of toxicity such as weight loss, diarrhea and vomiting in rats during the 28 day treatment period and four gypenoside derivatives, gynosaponin TN-1, gynosaponin TN-2, gypenoside XLV and gypenoside LXXIV were identified from GP-EX. These results suggest that GP-EX might be helpful in the prevention of Parkinson’s disease.
- Published
- 2010
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27. Inhibition of Cell Growth and Induction of Apoptosis via Inactivation of NF-κB by a Sulfurcompound Isolated From Garlic in Human Colon Cancer Cells
- Author
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Jung Ok Ban, Dong Yeon Yuk, Koan Sik Woo, Tae Myoung Kim, Ung Soo Lee, Heon-Sang Jeong, Dae Joong Kim, Yeun Bok Chung, Bang Yeon Hwang, Ki Wan Oh, and Jin Tae Hong
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Compounds such as S-allylmercaptocysteine, diallyl disulfide, and S-trityl-L-cysteine isolated from garlic have been known to be effective in chemoprevention. Nuclear transcription factor-κB (NF-κB) has been known to be an implicated factor in apoptotic cell death of several cancer cells. In this study, we investigated whether a sulfurcompound (named thiacremonone) isolated from garlic could modulate NF-κB activity and thereby induce apoptotic cell death of colon cancer cells. Treatment with different concentrations (30 – 150 µg/ml) of thiacremonone for various periods (0 – 48 h) inhibited colon cancer cell (SW620 and HCT116) growth followed by induction of apoptosis in a dose-dependent manner. We also found that thiacremonone modulated tumor necrosis factor-α (TNF-α) and tetradeanoyl phorbol acetate (TPA)-induced NF-κB transcriptional and DNA binding activity. Moreover, thiacremonone suppressed NF-κB target anti-apoptotic genes (Bcl-2, cIAP1/2, and XIAP) and inflammatory genes (iNOS and COX-2), whereas it induced apoptotic genes (Bax, cleaved caspse-3, and cleaved PARP) expression. These results suggest that a novel sulfurocompound from garlic inhibited colon cancer cell growth through induction of apoptotic cell death by modulating of NF-κB. Keywords:: thiacremonone, sulfurcompound, nuclear transcription factor-κB (NF-κB), colon cancer, apoptosis
- Published
- 2007
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28. Inositol Derivatives and Phenolic Compounds from the Roots of Taraxacum coreanum
- Author
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Eun Jin Mo, Jong Hoon Ahn, Yang Hee Jo, Seon Beom Kim, Bang Yeon Hwang, and Mi Kyeong Lee
- Subjects
Taraxacum coreanum ,inositol ,phenolic ,antioxidant ,Organic chemistry ,QD241-441 - Abstract
In this study, the characterization of chemical constituents and biological activity of the roots of Taraxacum coreanum (Asteraceae) was attempted. Phytochemical investigation of the roots of T. coreanum led to the isolation of two new inositol derivatives, taraxinositols A (1) and B (2), and a new phenolic compound, taraxinol (16), together with twenty known compounds including four inositol derivatives, neo-inositol-1,4-bis (4-hydroxybenzeneacetate) (3), chiro-inositol-1,5-bis(4- hydroxybenzeneacetate) (4), chiro-inositol-2,3-bis (4-hydroxybenzeneacetate) (5) and chiro-inositol- 1,2,3-tris (4-hydroxybenzeneacetate) (6), nine phenolic compounds: p-hydroxybenzaldehyde (7), vanillin (8), syringaldehyde (9), vanillic acid (10), 4-methoxyphenylacetic acid (11), 4-hydroxy- phenylacetic acid methyl ester (12), optivanin (13), isoferulic acid (14) and dihydroconiferyl alcohol (15), four coumarins: nodakenetin (17), decursinol (18), prangol (19) and isobyakangelicin (20), and three lignans: syringaresinol-4′-O-β-d-glucoside (21), syringaresinol (22), and pinoresinol (23). The structures of isolated compounds were determined on the basis of spectroscopic analysis. Among the isolated compounds, vanillic acid, isoferulic acid and syringaresinol showed radical scavenging activity with IC50 values ranging from 30.4 to 75.2 μM.
- Published
- 2017
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29. Anti-cancer effect of thiacremonone through down regulation of peroxiredoxin 6.
- Author
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Miran Jo, Hyung-Mun Yun, Kyung-Ran Park, Mi Hee Park, Dong Hun Lee, Seung Hee Cho, Hwan-Soo Yoo, Yong-Moon Lee, Heon Sang Jeong, Youngsoo Kim, Jae Kyung Jung, Bang Yeon Hwang, Mi Kyeong Lee, Nam Doo Kim, Sang Bae Han, and Jin Tae Hong
- Subjects
Medicine ,Science - Abstract
Thiacremonone (2, 4-dihydroxy-2, 5-dimethyl-thiophene-3-one) is an antioxidant substance as a novel sulfur compound generated from High-Temperature-High-Pressure-treated garlic. Peroxiredoxin 6 (PRDX6) is a member of peroxidases, and has glutathione peroxidase and calcium-independent phospholipase A2 (iPLA2) activities. Several studies have demonstrated that PRDX6 stimulates lung cancer cell growth via an increase of glutathione peroxidase activity. A docking model study and pull down assay showed that thiacremonone completely fits on the active site (cys-47) of glutathione peroxidase of PRDX6 and interacts with PRDX6. Thus, we investigated whether thiacremonone inhibits cell growth by blocking glutathione peroxidase of PRDX6 in the human lung cancer cells, A549 and NCI-H460. Thiacremonone (0-50 μg/ml) inhibited lung cancer cell growth in a concentration dependent manner through induction of apoptotic cell death accompanied by induction of cleaved caspase-3, -8, -9, Bax, p21 and p53, but decrease of xIAP, cIAP and Bcl2 expression. Thiacremonone further inhibited glutathione peroxidase activity in lung cancer cells. However, the cell growth inhibitory effect of thiacremonone was not observed in the lung cancer cells transfected with mutant PRDX6 (C47S) and in the presence of dithiothreitol and glutathione. In an allograft in vivo model, thiacremonone (30 mg/kg) also inhibited tumor growth accompanied with the reduction of PRDX6 expression and glutathione peroxidase activity, but increased expression of cleaved caspase-3, -8, -9, Bax, p21 and p53. These data indicate that thiacremonone inhibits tumor growth via inhibition of glutathione peroxidase activity of PRDX6 through interaction. These data suggest that thiacremonone may have potentially beneficial effects in lung cancer.
- Published
- 2014
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30. Polyacetylenes from the adventitious roots of Centella asiatica with glucose uptake stimulatory activity
- Author
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Yang Hee Jo, Sang Won Yeon, Jong Hoon Ahn, Ayman Turk, Qing Liu, Mun-Ock Kim, Bang Yeon Hwang, So-Young Park, and Mi Kyeong Lee
- Subjects
Bioengineering ,General Medicine ,Applied Microbiology and Biotechnology ,Biotechnology - Published
- 2023
31. Interruption of p38MAPK-MSK1-CREB-MITF-M pathway to prevent hyperpigmentation in the skin.
- Author
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Song-Hee Kim, Jiyeon Lee, Jihye Jung, Ga Hyun Kim, Cheong-Yong Yun, Sang-Hun Jung, Bang Yeon Hwang, Jin Tae Hong, Sang-Bae Han, Jae-Kyung Jung, and Youngsoo Kim
- Published
- 2024
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32. Chemical constituents from Neopestalotiopsis clavispora culture medium with estrogenic effects in MCF-7 cells
- Author
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Haeun Kwon, Quynh Nhu Nguyen, Seung Mok Ryu, Jaeyoung Kwon, Sojung Park, Yuanqiang Guo, Bang Yeon Hwang, Joung Han Yim, Jae-Jin Kim, Ki Sung Kang, and Dongho Lee
- Subjects
History ,Polymers and Plastics ,Plant Science ,Business and International Management ,Agronomy and Crop Science ,Biochemistry ,Industrial and Manufacturing Engineering ,Biotechnology - Published
- 2022
33. Hygrolansamycins A-D, O-Heterocyclic Macrolides from Streptomyces sp. KCB17JA11
- Author
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Jun-Pil Jang, Byeongsan Lee, Kyung Taek Heo, Tae Hoon Oh, Hyeok-Won Lee, Sung-Kyun Ko, Bang Yeon Hwang, Jae-Hyuk Jang, and Young-Soo Hong
- Subjects
General Medicine ,Applied Microbiology and Biotechnology ,Biotechnology - Published
- 2022
34. Targeting phosphorylation circuits on CREB and CRTCs as the strategy to prevent acquired skin hyperpigmentation.
- Author
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Song-Hee Kim, Changseon Na, Cheng-Yong Yun, Jun Gu Kim, Seung Tae Baek, Hyun Jin An, Jae Duk Lee, Seung Wha Lee, Jae-Kyung Jung, Bang Yeon Hwang, Sang-Bae Han, and Youngoo Kim
- Published
- 2024
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35. Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
- Author
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Lee, Ayman Turk, Solip Lee, Sang Won Yeon, Se Hwan Ryu, Yoo Kyong Han, Young Jun Kim, Sung Min Ko, Beom Seok Kim, Bang Yeon Hwang, Ki Yong Lee, and Mi Kyeong
- Subjects
Cordyceps militaris ,Mori Folium ,adenosine deaminase (ADA) ,cordycepin ,medicinal plants - Abstract
Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of Cordycepsmilitaris with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of Cordyceps to increase the cordycepin content. Cordyceps cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3′-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in C. militaris.
- Published
- 2023
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36. Molecular Networking-Guided Isolation of Cycloartane-type Triterpenoids from Curculigo orchioides and Their Inhibitory Effect on Nitric Oxide Production
- Author
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Jun Gu Kim, Thi Phuong Linh Le, Jae Sang Han, Yong Beom Cho, Dongho Lee, Mi Kyeong Lee, and Bang Yeon Hwang
- Subjects
General Chemical Engineering ,General Chemistry - Published
- 2022
37. Supplementary Data from Thiacremonone Augments Chemotherapeutic Agent–Induced Growth Inhibition in Human Colon Cancer Cells through Inactivation of Nuclear Factor-κB
- Author
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Jin Tae Hong, Sang Bae Han, Do Young Yoon, Dong Cheul Moon, Bang Yeon Hwang, Sugkil Song, Heon-Sang Jeong, Hee Soon Lee, and Jung Ok Ban
- Abstract
Supplementary Data from Thiacremonone Augments Chemotherapeutic Agent–Induced Growth Inhibition in Human Colon Cancer Cells through Inactivation of Nuclear Factor-κB
- Published
- 2023
38. Data from Thiacremonone Augments Chemotherapeutic Agent–Induced Growth Inhibition in Human Colon Cancer Cells through Inactivation of Nuclear Factor-κB
- Author
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Jin Tae Hong, Sang Bae Han, Do Young Yoon, Dong Cheul Moon, Bang Yeon Hwang, Sugkil Song, Heon-Sang Jeong, Hee Soon Lee, and Jung Ok Ban
- Abstract
Chemotherapeutic strategies commonly use multiple agents to overcome drug resistance and to lower drug toxicity. Activation of nuclear factor-κB (NF-κB) is implicated in drug resistance in cancer cells. Previously, we reported that thiacremonone, a novel sulfur compound isolated from garlic, inhibited NF-κB and cancer cell growth with IC50 values about 100 μg/mL in colon cancer cells. In the present study, we tested whether thiacremonone could increase susceptibility of cancer cells to chemotherapeutics through inactivation of NF-κB. Colon cancer cells were cotreated with thiacremonone (50 μg/mL, half dose of IC50) and lower doses of each chemotherapeutic agent (half dose of IC50) for 24 hours. NF-κB activity was completely abrogated in cells treated with a combination of thiacremonone and docetaxel, whereas thiacremonone on its own did not alter NF-κB activity. This combined drug effect was also found with other anticancer drugs in colon cancer and in other cancer cells. In good correlation with inhibition of cell growth and NF-κB activity, the combination treatment also regulated NF-κB target genes. Oral treatment of mice with thiacremonone (1 mg/kg) by administering it in drinking water for 4 weeks significantly augmented docetaxel (1 mg/kg, i.p., four times)–induced decrease of tumor growth accompanied with regulation of NF-κB activity and NF-κB target genes. These results warrant carefully designed clinical studies investigating the combination of thiacremonone and commonly used chemotherapeutic agents for the treatment of human cancers. (Mol Cancer Res 2009;7(6):870–9)
- Published
- 2023
39. Three new succinate-phenolic conjugates from the fruits of Actinidia arguta
- Author
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Jong Hoon Ahn, Sang Won Yeon, Se Hwan Ryu, Solip Lee, Ayman Turk, Bang Yeon Hwang, and Mi Kyeong Lee
- Subjects
Plant Science ,Agronomy and Crop Science ,Biochemistry ,Biotechnology - Published
- 2022
40. Construction of an Artificial Biosynthetic Pathway for Zingerone Production in Escherichia coli Using Benzalacetone Synthase from Piper methysticum
- Author
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Jungoh Ahn, Juhee Won, Kyung Taek Heo, Young-Soo Hong, Bang Yeon Hwang, Kyung Won Park, Byeongsan Lee, and Jae-Hyuk Jang
- Subjects
Zingerone ,ATP synthase ,biology ,Chemistry ,General Chemistry ,Reductase ,medicine.disease_cause ,Ferulic acid ,De novo synthesis ,chemistry.chemical_compound ,Biochemistry ,Polyketide synthase ,Caffeic acid ,biology.protein ,medicine ,General Agricultural and Biological Sciences ,Escherichia coli - Abstract
Zingerone (vanillylacetone; 4-hydroxy-3-methoxyphenylethyl methyl ketone) is a key component responsible for the pungency of ginger (Zingiber officinale). In this study, it was confirmed that a type III polyketide synthase (PKS) gene (pmpks) from Piper methysticum exhibits feruloyl-CoA-preferred benzalacetone synthase (BAS) activity. Based on these results, we constructed an artificial biosynthetic pathway for zingerone production from supplemented ferulic acid with 4-coumarate CoA ligase (4CL), PmPKS, and benzalacetone reductase (BAR). Furthermore, a de novo pathway for the production of zingerone was assembled using six heterologous genes, encoding tyrosine ammonia-lyase (optal), cinnamate-4-hydroxlase (sam5), caffeic acid O-methyltransferase (com), 4CL (4cl2nt), BAS (pmpks), and BAR (rzs1), in Escherichia coli. Using the engineered l-tyrosine-overproducing E. coli ΔCOS4 strain as a host, a maximum yield of 24.03 ± 2.53 mg/L zingerone was achieved by complete de novo synthesis.
- Published
- 2021
41. A new bibenzyl and a new methylflavan from the tubers of Bletilla striata
- Author
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Bang Yeon Hwang, Jin Woo Lee, Jun Gu Kim, Dongho Lee, and Mi Kyeong Lee
- Subjects
Circular dichroism ,biology ,Data interpretation ,Plant Science ,biology.organism_classification ,Biochemistry ,Nitric oxide ,chemistry.chemical_compound ,chemistry ,Bletilla striata ,Ic50 values ,Bibenzyl ,Agronomy and Crop Science ,Two-dimensional nuclear magnetic resonance spectroscopy ,Biotechnology ,Nuclear chemistry - Abstract
A new bibenzyl, (+)-(1S)-4ʹ,5ʺ-dihydroxy-1,3ʹ,5ʹ,3ʺ-tetramethoxybibenzyl (1), and a new methylflavan, (2S)-7,4′-dihydroxy-5-methoxy-8-methylflavan (2), together with 10 known compounds were isolated from the methanolic extract of the tubers of Bletilla striata. Their chemical structures were elucidated based on spectrometric data interpretation, especially 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data. All isolates (1 - 12) were evaluated for their inhibitory properties on nitric oxide production induced in RAW 264.7 cells, and dihydrophenantrenes 4 and 6 exhibited the best inhibitory effects with IC50 values of 29.5, and 6.5 μM, respectively. Additionally, significant activity possessed phenantrene 5 and methylflavane 2 with IC50 values of 38.9, and 44.9 μM, respectively.
- Published
- 2021
42. Phenyldilactones from the leaves of hardy kiwifruit (Actinidia arguta)
- Author
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Jong Hoon Ahn, Se Hwan Ryu, Sang Won Yeon, Solip Lee, Seon Beom Kim, Bang Yeon Hwang, and Mi Kyeong Lee
- Subjects
Biochemistry ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
43. Hygrolansamycins A-D
- Author
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Jun-Pil, Jang, Byeongsan, Lee, Kyung Taek, Heo, Tae Hoon, Oh, Hyeok-Won, Lee, Sung-Kyun, Ko, Bang Yeon, Hwang, Jae-Hyuk, Jang, and Young-Soo, Hong
- Subjects
Molecular Structure ,Lactams, Macrocyclic ,Macrolides ,Streptomyces ,Anti-Bacterial Agents - Abstract
Six ansamycin derivatives were isolated from the culture broth of
- Published
- 2022
44. Sesquiterpenoids from Chrysanthemum indicum with Inhibitory Effects on NO Production
- Author
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Thi Phuong Linh Le, Jin Woo Lee, Haeun Kwon, Bang Yeon Hwang, Dongho Lee, Yong Beom Cho, Jun Gu Kim, Jae Sang Han, and Mi Kyeong Lee
- Subjects
Pharmacology ,chemistry.chemical_classification ,Circular dichroism ,biology ,010405 organic chemistry ,Chemistry ,Stereochemistry ,Organic Chemistry ,Pharmaceutical Science ,Inhibitory postsynaptic potential ,biology.organism_classification ,01 natural sciences ,Flavones ,0104 chemical sciences ,Analytical Chemistry ,Nitric oxide ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,Drug Discovery ,Ic50 values ,Molecular Medicine ,Acid hydrolysis ,Chrysanthemum indicum ,No production - Abstract
Three new guaianolide lactones (1-3) and four new 9-oxonerolidol glucosides (5-8) together with 20 known compounds were isolated from the MeOH extract of the flowers of Chrysanthemum indicum. Their structures were elucidated based on the interpretation of NMR, HRESIMS, and electronic circular dichroism (ECD) data along with acid hydrolysis. Of the isolates, sesquiterpenoids 1-4 and 15 and flavones 17 and 18 exhibited inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide production in RAW 264.7 cells with IC50 values in the range 0.2-27.0 μM.
- Published
- 2021
45. Tetrahydroprotoberberine N-oxides from Chelidonium majus and their inhibitory effects on NO production in RAW 264.7 cells
- Author
-
Jun Gu Kim, Jin Woo Lee, Thi Phuong Linh Le, Haeun Kwon, Dongho Lee, Mi Kyeong Lee, Bang Yeon Hwang, and Jae Sang Han
- Subjects
Chromatography ,biology ,010405 organic chemistry ,Chemistry ,Plant Science ,biology.organism_classification ,Inhibitory postsynaptic potential ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Nitric oxide ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Ic50 values ,Papaveraceae ,Chelidonium ,No production ,Agronomy and Crop Science ,Two-dimensional nuclear magnetic resonance spectroscopy ,RAW 264.7 Cells ,Biotechnology - Abstract
Two new tetrahydroprotoberberine N-oxides, 7R,14S-cis-tetrahydrocoptisine N-oxide (1) and 7R,14R-trans-tetrahydrocoptisine N-oxide (2) along with fourteen known compounds (3–16) were isolated from the aerial parts of Chelidonium majus. Their structures were elucidated by spectroscopic and spectrometric methods, such as 1D and 2D-NMR (HSQC, HMBC, NOESY) and HRESIMS. The absolute configurations of 1 and 2 were established by comparison of their experimental and calculated ECD data. All isolates were evaluated for their inhibitory effects on the nitric oxide production in LPS-induced RAW 264.7 macrophages, and compounds 2, 6–10, 13, and 15 showed significant inhibitory effects with the IC50 values ranging from 1.1–31.9 μM.
- Published
- 2021
46. Targeted isolation of sesquiterpene lactone dimers from Aucklandia lappa guided by LC-HRMS/MS-based molecular networking
- Author
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Jae Sang Han, Jun Gu Kim, Thi Phuong Linh Le, Yong Beom Cho, Dongho Lee, Jin Tae Hong, Mi Kyeong Lee, and Bang Yeon Hwang
- Subjects
Plant Science ,General Medicine ,Horticulture ,Molecular Biology ,Biochemistry - Abstract
An LC-HRMS/MS-based molecular networking strategy was applied to investigate the potential sesquiterpene dimers of Aucklandia lappa, leading to the isolation of three undescribed guaiane-guaiane dimers and one guaiane-eudesmane dimer together with six known sesquiterpenes. The structures were determined by analyzing their 1D, 2D NMR, and HRESIMS data as well as ECD calculations. The biogenetic pathway of the sesquiterpene dimers was postulated to involve the Diels-Alder cycloaddition as the key step. All compounds exhibited their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages with IC
- Published
- 2023
47. Diterpenoids and Diacetylenes from the Roots of Aralia cordata with Inhibitory Effects on Nitric Oxide Production
- Author
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Dongho Lee, Haeun Kwon, Thi Phuong Linh Le, Jin Tae Hong, Jun Gu Kim, Jin Woo Lee, Youngsoo Kim, Bang Yeon Hwang, Mi Kyeong Lee, and Jae Sang Han
- Subjects
Pharmacology ,Circular dichroism ,biology ,Diacetylene ,010405 organic chemistry ,Stereochemistry ,Organic Chemistry ,Pharmaceutical Science ,biology.organism_classification ,Inhibitory postsynaptic potential ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Nitric oxide ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,Ic50 values ,Molecular Medicine ,Aralia cordata - Abstract
Bioactivity-guided isolation of a MeOH extract of Aralia cordata led to the isolation of four new ent-pimarane diterpenoids (1-4) and a diacetylene (5) together with 21 known compounds (6-26). Their structures were established based on the interpretation of one- and two-dimensional NMR and HRESIMS data. The absolute configurations of the new isolates were determined by electronic circular dichroism data analysis, single crystal X-ray diffraction, and Mosher's esterification method. All compounds exhibited inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages with IC50 values ranging from 1.1 to 69.4 μM.
- Published
- 2021
48. Chemical constituents from Pterocarpus santalinus and their inhibitory effects on nitric oxide production
- Author
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Jae Sang Han, Hye Ryeong Hong, Jun Gu Kim, Thi Phuong Linh Le, Yong Beom Cho, Mi Kyeong Lee, and Bang Yeon Hwang
- Subjects
Pharmacology ,History ,Polymers and Plastics ,Molecular Structure ,Pterocarpus ,Macrophages ,Drug Discovery ,General Medicine ,Business and International Management ,Nitric Oxide ,Sesquiterpenes ,Industrial and Manufacturing Engineering - Abstract
A tropolone (2) and an acorane sesquiterpene (3), along with twenty previously known compounds were isolated from the heartwood of Pterocarpus santalinus. The structure of the isolated compounds was elucidated via 1D and 2D NMR spectroscopy and HRESIMS analysis. The absolute configuration of 3 was determined by comparison of the experimental and calculated ECD data. All compounds were evaluated for their inhibitory effects against nitric oxide production in LPS-stimulated RAW 264.7 macrophages.
- Published
- 2022
49. Polyacetylenes from the roots of Cirsium japonicum var. ussuriense
- Author
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Seung Hyun Lee, Jun Gu Kim, Thi Phuong Linh Le, Jae Sang Han, Yong Beom Cho, Mi Kyeong Lee, Dongho Lee, and Bang Yeon Hwang
- Subjects
Molecular Structure ,Macrophages ,Polyynes ,Plant Science ,General Medicine ,Horticulture ,Nitric Oxide ,Molecular Biology ,Biochemistry ,Cirsium ,Polyacetylene Polymer - Abstract
Eight previously undescribed polyacetylenes, cirussurynes A-H, were isolated from the methanolic extract of the roots of Cirsium japonicum var. ussuriense. Their structures were elucidated by interpretation of extensive 1D and 2D NMR spectroscopy and HRESIMS spectrometry data. The configuration of triols in cirussurynes A, B, and E-G was deduced by the J-value based configuration analysis together with specific rotation values. All compounds were evaluated for their inhibitory effects on nitric oxide production against LPS-induced RAW 264.7 macrophages, and exhibited IC
- Published
- 2022
50. Revolutionizing technologies of nanomicelles for combinatorial anticancer drug delivery
- Author
-
Dae Hwan Shin, Jin-Seok Kim, Ik Sup Jin, Bang Yeon Hwang, Chun-Woong Park, Youn Bok Chung, and Min Jeong Jo
- Subjects
0301 basic medicine ,Polymeric micelles ,business.industry ,Organic Chemistry ,Pharmacology toxicology ,Antineoplastic Agents ,Nanotechnology ,Anticancer drug ,03 medical and health sciences ,Drug Delivery Systems ,030104 developmental biology ,0302 clinical medicine ,Solubilization ,030220 oncology & carcinogenesis ,Drug Discovery ,Drug delivery ,Animals ,Humans ,Nanoparticles ,Molecular Medicine ,Medicine ,business ,Micelles - Abstract
Insufficient efficacy of current single drug therapy of cancers have led to the advancement of combination drug-loaded formulations. Specifically, polymeric micelles have been focused on as efficient injectable vehicles for the delivery of several anticancer drugs simultaneously to cancer cells. These nano delivery systems have evolved with advancements in the area of nanotechnology. The current review presents a summary of the past events that have led to the procession of nanomicelles and novel nanotechnologies for combinatorial drug delivery. It also focuses on the advantages, disadvantages, and considerations for the design of nanotechnologies for combinatorial drug delivery systems. The opportunities and challenges of nanotechnologies in drug delivery to overcome current disadvantages are also discussed. Furthermore, we have added findings regarding the trends and perspectives regarding nanotechnologies for combinatorial anticancer drug delivery.
- Published
- 2020
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