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Clock genes play a pivotal role in the rhythm maturation of bony fish ovaries. To explore the role of clock genes in the rhythmic ovarian development and maturation of tongue sole (Cynoglossus semilaevis), we used real-time fluorescent quantitative PCR technique to analyze the expression profiles of circadian locomotor output cycles Kaput 1a (Clock1a), brain and muscle Arntlike 1a (Bmal1a), Cryptochrome 1a (Cry1a), Cryptochrome 2 (Cry2), and Period 2 (Per2) at the ovarian stages Ⅱ, Ⅲ, Ⅳ, Ⅴ, and Ⅵ.The coding DNA sequence (CDS) of five clock genes were cloned and phylogenetically analyzed. We found that the CDS sequence length of Clock1a was 1 620 bp and encoded 539 amino acids, with the encoded amino acid sequence of Clock1a having a predicted molecular weight of 81.9 kDa. Clock1a has the functional domain PASD1 (consisting of 64 amino acids) and PAS11 (consisting of 103 amino acids). The CDS sequence length of Bmal1a was 1 881 bp and encoded 626 amino acids, with the encoded amino acid sequence of Bmal1a having a predicted molecular weight of 68.9 kDa. In the Bmal1a sequence, the functional domain PASD3 is composed of 63 amino acids, and the functional domain PAS11 is composed of 103 amino acids. The CDS sequence length of Cry1a was 1 896 bp and encoded 631 amino acids, with the encoded amino acid sequence of Cry1a having a predicted molecular weight of 71.4 kDa. Its functional domain FAD7 was composed of 199 amino acids. The CDS sequence length of Cry2 was 2 007 bp and encoded 669 amino acids, with the encoded amino acid sequence of Cry2 having a predicted molecular weight of 76.0 kDa. The Cry2 sequence contains a functional domain FAD7 consisting of 199 amino acids. The CDS sequence length of Per2 was 4 248 bp, encoding 1 415 amino acids, and the encoded amino acid sequence of Per2 has a predicted molecular weight of 154.0 kDa. In the Per2 sequence, the functional domain PeriodC consists of 295 amino acids and a functional domain PAS11 consists of 102 amino acids.The neighbor-joining method was used to analyze the Clock1a, Bmal1a, Cry1a, Cry2, and Per2 phylogenetic relationships between C. semilaevis and other bony fish, amphibians, birds, and mammals. The homology of Clock1a, Bmal1a, Cry1a, Cry2, and Per2 with other bony fish was 60%–79%, 94%–100%, 85%–91%, 84%–94%, and 70%–84%, respectively. Therefore, we believe that these five amino acid sequences show strong conserved property. In addition, the homology of Cry1a and Cry2 was 62%, indicating that Cry1a and Cry2 evolved differently during the evolution of C. semilaevis. In the constructed phylogenetic tree, Clock1a, Bmal1a, Cry1a, Cry2, and Per2 of C. semilaevis were clustered together with other bony fishes, indicating a close relationship between C. semilaevis and other bony fish in the evolutionary process. Moreover, the homology of Clock1a between C. semilaevis and mammals, birds, and amphibians is low, indicating that there are evolutionary differences in the evolutionary process of Clock1a. The high homology of Bmal1a, Cry1a, Cry2, and Per2 with mammals, birds, and amphibians suggests that these four clock genes were strongly conserved during the evolution of C. semilaevis.In the present study, we found that the expression levels of five clock genes were high in stagesⅡ and Ⅲ, which were equivalent to the non-reproductive season (P < 0.05). However, the expression levels of five clock genes were low in stages Ⅳ and Ⅴ, which were equivalent to the reproductive season (P < 0.05). Therefore, the expression profiles of Clock1a, Bmal1a, Cry1a, Cry2, and Per2 in the ovaries of C. semilaevis also have seasonal characteristics. The ovarian development and maturation of C. semilaevis goes through stages Ⅱ, Ⅲ, Ⅳ, Ⅴ, and Ⅵ, and then reaches stage Ⅱ again and starts a new reproductive cycle. The variation patterns of seasonal factors, such as light and temperature, in fish ovaries were consistent with the annual expression patterns of the five clock genes in this study. Therefore, it can be considered that the expression of clock genes in C. semilaevis has an annual cycle. The findings presented in this study can enrich the theory of ovarian development and maturation of C. semilaevis and provide a theoretical basis for improving breeding technology and seedling efficiency.

The whitespotted conger (Conger myriaster) is an important fish species with high economic value in China and Japan owing to its high nutritional content. It occupies an important position in marine-eel export in China. C. myriaster has the potential to be artificially cultured and has promising economic prospects. Thus, there is growing international interest in C. myriaster research. Indeed, to meet the demands of domestic and foreign markets, artificial culturing of C. myriaster is urgently required. However, at present, there is no report on the research of C. myriaster reared in industrial recirculating aquaculture systems. Feeding frequency has been reported to affect feed intake and growth performance in a number of fish species. Feed management in terms of optimization of feeding frequency has emerged as one of the crucial areas of research in the field of aquaculture. Overfeeding and leftover food disrupts the water quality, while inadequate food supply has direct impact on production cost. Traditional flow through systems uses large volumes of water, and intensified aquaculture has drawbacks, such as large amount of discharged waste from effluent water. Among the excreted nutrients, nitrogen and phosphorus constitute the largest proportion. Sustainability with low environmental impacts is one of the concerns when developing aquaculture systems. Based on the concepts of reduced water consumption and efficient water management, recirculating aquaculture systems offer the possibility of creating a sustainable future for fish production. Thus, a 70-day experiment was conducted to investigate the effects of different feeding frequencies and water quality on the growth performance and physiological and biochemical indices of C. myriaster [body weight: (150.64±5.43) g] reared in industrial recirculating aquaculture systems; physiological and ecological methods were used in this study. The experiment comprised two feeding frequency groups, feeding once (group T1) and twice (group T2) per day, and each treatment group had three replicates. The average body weight of the fish in each culture pond and the water quality indices of the culture system were measured and analyzed every 14 d. At the end of the experiment, the blood, liver, and intestine samples of C. myriaster were collected to determine and analyze growth and physiological indices. The results indicated that (1) the growth performance of group T2 was better than that of group T1. The final body weight, final body length, weight gain, and specific growth rate of group T2 was significantly higher than those of group T1 (P < 0.05). The feed coefficient of group T1 was significantly higher than that of group T2 (P < 0.05). However, no significant differences were found in the final body height, liver body ratio, visceral body ratio, condition factor, or survival rate at different feeding frequencies (P > 0.05). (2) The serum malondialdehyde of group T1 was significantly higher than that of group T2 (P < 0.05). The hepatic alanine aminotransferase and hepatic aspartate transaminase of group T1 was significantly higher than those of group T2 (P < 0.05). The intestinal trypsin of group T2 was significantly higher than that of group T1 (P < 0.05). There were no significant differences in the levels of serum superoxide dismutase, serum catalase, intestinal amylase, or intestinal lipase between groups T1 and T2 (P > 0.05). (3) In the 24 h changes of ammonia nitrogen (NH4+-N) and nitrite nitrogen (NO2–-N), the change patterns of the two indices in outlet of the T1 group were similar to those of the outlet of the bio-filter; the two indices of the outlet water in T1 and T2 groups were one (24:00) and two peaks (20:00 and 08:00) respectively; the difference in NH4+-N in the outlet water of the two test groups within 24 h was significant (P < 0.05); at 04:00 and 08:00, the NO2–-N content of the outlet water of the biofilter was significantly lower than that of the other two test groups (P < 0.05); the 24 h changes range of NH4+-N and NO2–-N in T2 group were smaller than those in T1 group. (4) During the whole test period, each water quality index was maintained within the safe breeding range, and no significant differences were found in NH4+-N, NO2–-N, chemical oxygen demand, and active phosphate concentration between T1 and T2 groups (P > 0.05); at the end of the test, the total nitrogen and total phosphorus of T2 group were significantly higher than those of T1 group (P < 0.05); the biofilter has different treatment effects on various water quality indices, among which the treatment effect on NH4+-N is the most efficient, up to 54.23%. Based on the above results, the feeding frequency of twice per day was better than that of once per day in the industrial recirculating aquaculture mode for C. myriaster (body weight: 150~250 g). Without affecting the survival rate and condition factor, the twice per day group can save costs and improve economic benefits. However, due to the limitations of the test conditions, we did not explore more times of feeding, and further tests are needed in the future. The predominance, which was the high efficiency of water qualities treatment and optimal effect of culture, was demonstrated obviously in closed recirculating aquaculture systems. This study aims to provide a theoretical reference for the appropriate feeding strategy in the industrial recirculating aquaculture model of C. myriaster.

Abstract A series of terpenoids (1–17), comprising six new compounds designated bipolariterpenes A-F (1–6) and eleven recognized compounds (7–17), were isolated from the wheat culture of the potato endophytic fungus Bipolaris eleusines. Their structures and stereochemistry were clarified by HRESIMS, NMR, DP4 + probability analyses, and computations for electronic circular dichroism (ECD). All compounds are made up of six meroterpenoids, four sesterterpenes and seven sesquiterpenes. Among them, four sesterterpenes (4, 5, 10, 11) were investigated for their antifungal, antibacterial and cytotoxic properties, and six meroterpenoids (1–3, 7–9) were evaluated for their antifungal properties. The compounds 7, 9, and 10 had substantial antifungal activity against Epidermophyton floccosum at a concentration of 100 µM. No antibacterial and cytotoxic activities were observed. Graphical Abstract

Gravity field is the most basic physical field generated by the material properties of the Earth system. It reflects the spatial distribution, movement and change of materials determined by the interaction and dynamic process inside the Earth. Over the years, a variety of technical means have been used to detect the Earth’s gravity field and supported numerous studies on the global change, resource detection, geological structure movement, water resources change and other related fields of research. Here is part of the progress in surface and marine gravimetry obtained by Chinese geodesy scientists from 2019 to 2023 from the following aspects, including: ① Continuous gravity network in Chinese mainland; ② Application of superconducting gravity measurement; ③ Network adjustment for continental-scale gravity survey campaign and data quality control; ④ Regional time-variable gravity field and its application; ⑤ Research progress on novel technologies for gravity inversion; ⑥ Research progress on marine gravity field determination; ⑦ Application research on marine gravity field.

BackgroundUltrasound-guided percutaneous device closure of perimembranous ventricular septal defects (PmVSD) is a minimally invasive recent treatment approach. Perventricular PmVSD device closure is an emerging radiation-free intervention, yet it comes with certain limitations. No studies compared both of these treatment approaches.MethodsWe performed a retrospective institutional data comparison of percutaneous (PCP Group, n = 138) and perventricular (PVP Group, n = 67) ultrasound-guided device closure procedures in 205 patients with PmVSD between March 2017 and December 2022.ResultsPatients of the PCP and PVP groups had a median age of 4.9 years (IQR, 3.1–14.0) and 5.3 years (IQR, 3.4–13.1) respectively. The median PmVSD diameter in the PCP Group was 4.0 mm (IQR, 3.3–5.3) and 5.2 mm (IQR, 4.0–7.0) in the PVP Group (p = 0.001). There was no significant difference in success rates between the PCP and PVP Groups (intention-to-treat population, 88.4% vs. 92.5%, p = 0.36; as-treated population, 88.4% vs. 89.3%, p = 0.84). 5/8 failed percutaneous cases that were shifted to the perventricular approach were successful. Compared to the PVP Group, patients of the PCP group experienced a significant decrease in ventilation time, drainage volume, and postoperative hospital stay (p

Accurate rotation of microparticles is of great significance in micro-rotors, multi-angle microscopic observation, microbial three-dimensional phenotyping, and microsystem assembly. However, most methods can only rotate a single object, thus limiting the throughput. In this study, we realized the simultaneous rotation of many trapped and aligned subwavelength glass cylinders inside an evanescent wave field excited by a resonant phononic crystal plate. The unique feature of the rotation lies in its periodic distribution as well as the rotation axis being perpendicular to the acoustic axis. The rotary power originates from viscous torque generated by the evanescent wave-induced near-boundary acoustic streaming's asymmetry distribution on the trapped cylinder. Furthermore, the three-dimensional topographies of rotated cylinders can be reconstructed from the microscopic images under different rotating angles. Our findings can pave the way toward developing simple, disposable, and scalable microfluidic devices for massive subwavelength acoustic rotation by carefully designing acoustic metamaterials. [ABSTRACT FROM AUTHOR]

Abstract Three hitherto undescribed Stemona alkaloids, named stemajapines A–C (1–3), along with six known alkaloids (4–9), were isolated and identified from the roots of Stemona japonica (Blume) Miq. (Stemonaceae). Their structures were established by the analysis of the mass data, NMR spectra, and computational chemistry. Stemjapines A and B were degraded maistemonines without spiro-lactone ring and skeletal methyl from maistemonine. Concurrence of alkaloids 1 and 2 revealed an undescribed way to form diverse Stemona alkaloids. Bioassay results disclosed the anti-inflammatory natural constituents stemjapines A and C with IC50 values of 19.7 and 13.8 µM, respectively, compared to positive control dexamethasone with 11.7 µM. The findings may point out a new direction of Stemona alkaloids inaddition to its traditional antitussive and insecticide activities.

Meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Meniscus injuries are difficult to heal and frequently progress into structural breakdown, which then leads to osteoarthritis. Regeneration of heterogeneous tissue engineering meniscus (TEM) continues to be a scientific and translational challenge. The morphology, tissue architecture, mechanical strength, and functional applications of the cultivated TEMs have not been able to meet clinical needs, which may due to the negligent attention on the importance of microenvironment in vitro and in vivo. Herein, we combined the 3D (three-dimensional)-printed gradient porous scaffolds, spatiotemporal partition release of growth factors, and anti-inflammatory and anti-oxidant microenvironment regulation of Ac2-26 peptide to prepare a versatile meniscus composite scaffold with heterogeneous bionic structures, excellent biomechanical properties and anti-inflammatory and anti-oxidant effects. By observing the results of cell activity and differentiation, and biomechanics under anti-inflammatory and anti-oxidant microenvironments in vitro, we explored the effects of anti-inflammatory and anti-oxidant microenvironments on construction of regional and functional heterogeneous TEM via the growth process regulation, with a view to cultivating a high-quality of TEM from bench to bedside.

Wearable flexible pressure sensors are a novel class of human activity detection device. However, the preparation of pressure sensors with high sensitivity and wide sensing range still faces great challenges. This study reveals an flexible heat-resistant variable density point pressure sensing array (PSA) with ultra-wide sensing range based on Ti3C2Tx-MXene. MXene containing polar oxygen-containing functional groups coated polyester fiber fabricated the pressure sensing layer while a stainless steel wire core is used as a flexible electrode for signal collection. The signal processing device rapidly converts the mechanical signal into electrical signal output to increase the transmission speed and range. Experimental results show that the PSA can effectively sense dynamic and static pressures with high sensitivity (0.14–0.87 kPa−1 over a pressure range of 7.2 Pa–2000 kPa), a wide sensing range (0–15000 kPa), fast response time (80 ms), 10,000 cycles (2000 kPa) stability and maintains a 81.25% current response. The noval fully woven structural flexible PSA exhibited larger area, of which the weave density is varied to control resolution and the pressure mapping is referenced to each pixel point to qualitatively and quantitatively analyze shape and pressure distribution. Variable density points pressure sensor with wide sensing range and spatial pressure mappinghas promising applications in healthcare.

Abstract Backgrounds Hyperlactatemia is a common metabolic disorder after cardiac surgery with cardiopulmonary bypass. Epinephrine use has been identified as a potential cause of increased lactate levels after cardiac surgery. Stress can lead to an increase in catecholamines, mainly epinephrine, in the body. Exogenous epinephrine causes hyperlactatemia, whereas endogenous epinephrine released by stress may have the same effect. Opioids are the most effective anesthetics to suppress the stress response in the body. The authors sought to provide evidence through a retrospective data analysis that helps investigate the relationship between intraoperative opioid dosage and postoperative lactic acidosis after cardiac surgery. Methods The clinical data of 215 patients who underwent valvular heart surgery with cardiopulmonary bypass from July 2016 to July 2019 were analyzed retrospectively. Blood lactate levels were measured at 0.1 h, 2 h, 4 h, and 8 h after surgery. Patients with continuous increases in lactate levels and lactate levels exceeding 5 mmol/L at two or more time points were included in the lactic acidosis group, whereas the other patients were included in the control group. First, univariate correlation analysis was used to identify parameters that were significantly different between the two groups, and then multivariate regression analysis was conducted to elucidate the independent risk factors for lactic acidosis. Fifty-one pairs of patients were screened by propensity score matching analysis (PSM). Then, lactic acid levels at four time points in both groups were analyzed by repeated measures ANOVA. Results he EF (heart ejection fraction) (OR = 0.94, P = 0.003), aortic occlusion time (OR = 10.17, P

Moderate clothes-changing person re-identification is to find the same person from different camera scenes under the premise of considering the moderate change of clothes.The implementation of existing person re-identification methods is usually based on the assumption that the pedestrian’s clothing is invariant,so they rely on clothing-related features.Then,when the above assumptions are not valid,these methods are difficult to achieve the ideal recognition performance.Considering the important characteristic that pedestrian’s shape hardly change when the change of clothes is moderate,the moderate clothes-changing person re-identification is studied.Inspired by the binocular summation in biological vision system,a self-attention siamese network is proposed in this paper.Analogous to biological binocular information acquisition process,the network takes two types of images of the same pedestrian with different clothes as two branch inputs,and then achieves summation effect with siamese architecture.Subsequently,the contrastive learning and fusion learning of multiple features are carried out to obtain the pedestrian feature representation with identity discrimination.Finally,empirical studies show that the proposed method achieves best performance at present on clothes-changing person re-identification benchmark.

Abstract Five undescribed alkaloids were isolated from the seeds of Cephalotaxus oliveri along with 27 known ones. The new structures were elucidated based on spectroscopic data including 1D and 2D NMR, MS and calculated ECD spectra. Among them, (+)-acetylcephalofortine C was an enantiomeric Cephalotaxine alkaloids. The performed bioassay revealed that those alkaloids were not cytotoxic against cancer cells and had no neuroprotective properties in the HEI-OC-1 cells model.

Objective: In this study, we compared the enhancement of blood vessels and liver parenchyma on enhanced computed tomography (CT) of the upper abdomen with two concentrations of contrast media (400 and 300 mg I/mL) based on similar iodine delivery rate (IDR) of 0.88 and 0.9 g I/s and iodine load of 450 mg I/kg.Methods: We randomly assigned 160 patients into two groups: iomeprol 400 mg I/mL (A group) and iohexol 300 mg I/mL (B group). The CT attenuation values of the main anatomical structures in the two groups with different scanning phases were measured and the image quality of the two groups was analyzed and compared. The peak pressure and local discomfort (including fever and pain) during contrast medium injection were recorded.Results: The mean attenuation value of the abdominal aorta was 313.6 ± 29.6 in the A group and 322.4 ± 30.1 in the B group during the late arterial phase (p = 0.8). Meanwhile, the mean enhancement values of the portal vein were 176.2 ± 19.3 and 165.9 ± 24.5 in the A and B groups, respectively, during the portal venous phase (p = 0.6). The mean CT values of liver parenchyma were 117.1 ± 15.3 and 108.8 ± 18.7 in the A and B groups, respectively, during the portal venous phase (p = 0.9). There was no statistical difference in image quality, peak injection pressure (psi), and local discomfort between the two groups (p > 0.05).Conclusion: When a similar IDR and the same iodine load are used, CT images with different concentrations of contrast media have the same subjective and objective quality, and can meet the diagnostic needs.

Coal is the main energy source in China. As flat and shallow coal seams are being depleted, adverse coal seams such as inclined and steeply inclined coal seams account for larger proportion of seams that are mined. For these coal seams, instability such as slip and tipping of mining equipment due to the large inclination is a significant challenge for the productive operation of intelligent or smart mines. Therefore, this paper serves to provide some insights into improving their stability. In this paper, research on the anti-tipping and anti-slip technology of shields is carried out on an intelligent working face with a large dip angle. A mechanical model of “support-surrounding rock” was established. Through the analysis of the influence of the self-weight of the support on its stability and through theoretical analysis and field practice, it was found that the critical tipping angle of the support in the free state is 27.8, the critical slip angle is 16, and the support is more prone to slip in the free state; the shields in the middle of the working face are the key area for stability control. Suitable technical measures are taken to ensure the stability of the supports, which provides the management and practical basis for safe and efficient mining in the intelligent working face with a large dip angle.

Highlights Ti3C2T x MXene-based coaxial zinc-ion hybrid fiber supercapacitors (FSCs) were fabricated with braided structure, which can be prepared continuously and present excellent flexibility and ultrastability. A sports watch driven by the watch belts which weaved uses the obtained zinc-ion hybrid FSC and LED arrays lighted by the FSCs under embedding into textiles, demonstrating the great potential application in smart wearable textiles. Abstract Zinc-ion hybrid fiber supercapacitors (FSCs) are promising energy storages for wearable electronics owing to their high energy density, good flexibility, and weavability. However, it is still a critical challenge to optimize the structure of the designed FSC to improve energy density and realize the continuous fabrication of super-long FSCs. Herein, we propose a braided coaxial zinc-ion hybrid FSC with several meters of Ti3C2T x MXene cathode as core electrodes, and shell zinc fiber anode was braided on the surface of the Ti3C2T x MXene fibers across the solid electrolytes. According to the simulated results using ANSYS Maxwell software, the braided structures revealed a higher capacitance compared to the spring-like structures. The resulting FSCs exhibited a high areal capacitance of 214 mF cm–2, the energy density of 42.8 μWh cm−2 at 5 mV s−1, and excellent cycling stability with 83.58% capacity retention after 5000 cycles. The coaxial FSC was tied several kinds of knots, proving a shape-controllable fiber energy storage. Furthermore, the knitted FSC showed superior stability and weavability, which can be woven into watch belts or embedded into textiles to power smart watches and LED arrays for a few days.

Most of the images encountered in daily life are color images, yet grayscale remains prevalent in many fields due to its reduced data size and simplified operations. When reducing the dimensions of a three-channel color image to a single-channel grayscale, a portion of the original color information is inevitably lost. To yield high-quality grayscale images, this study introduces a color-to-gray conversion method based on chroma difference. This method defines a novel color distance metric for color-to-grayscale conversion, incorporating pixel chromaticity differences alongside brightness variations. The discrepancy in gray pixel values in the output grayscale image effectively mirrors the overall differences among input color image pixels. Optimization is conducted using the conjugate gradient method, ensuring appropriate reflection of luminance information from the input image and chromaticity data from the original color image within the grayscale rendering. Experimental validation confirms the efficacy of this approach. However, as the method accounts for all pixel pairs, it occasionally considers unnecessary pairs, leading to potential distortion in color differences between pixels and consequent inadequacies in chromaticity variation. To address this issue, a weighting factor is introduced, prioritizing color combinations with similar color differences. Experimental findings demonstrate that grayscale images produced using the proposed method outperform those generated by alternative approaches in preserving color differentials and retaining detailed features of the original image. The resulting output exhibits clear and natural outlines, as supported by both subjective and objective assessments. [ABSTRACT FROM AUTHOR]

A total of eleven new dimeric chromanones, paecilins F-P (2–12), were isolated from the endophytic fungus Xylaria curta E10, along with four known analogs (1, 13–15). Their structures and absolute configurations were determined by extensive experimental spectroscopic methods, single-crystal X-ray diffraction, and equivalent circulating density (ECD) calculations. In addition, the structure of paecilin A, which was reported to be a symmetric C8-C8′ dimeric pattern, was revised by analysis of the nuclear magnetic resonance (NMR) data, and single-crystal X-ray diffraction. Compound 1 showed antifungal activity against the human pathogenic fungus Candida albicans with a minimum inhibitory concentration of 16 μg/mL, and Compounds 8 and 10 showed antibacterial activity against the gram-negative bacterium Escherichia coli with the same minimum inhibitory concentration of 16 μg/mL.

Abstract Background The stem cells of the stem cell banks have prominent problems for insufficient sources, easy contamination, unstable biological characteristics after serial subcultivations, and high cost. Methods After collecting the construction processes of the existing stem cell banks and suggestions from authoritative experts in the past 10 years, 230 reference principles were obtained, and finally, the principles of “5C” for the establishment of modern standardized stem cell banks were summarized, and their related applications on the management of sports injuries were reviewed as well. Results The basic principles of “5C” for the establishment of modern standardized stem cell banks include (1) principle of informed consent, (2) confidentiality principle, (3) conformity principle, (4) contamination-free principle, and (5) commonweal principle. The applications of stem cells on repairs, reconstructions, and regenerations of sports injuries were also reviewed, especially in tissue-engineered cartilage, tissue-engineered meniscus, and tissue-engineered ligament. Conclusions The proposal of the basic principles of “5C” is conducive to relevant stem cell researchers and clinical medical experts to build modern stem cell banks in a more standardized and efficient manner while avoiding some major mistakes or problems that may occur in the future. On this basis, stem cells from stem cell banks would be increasingly used in the management of sports injuries. More importantly, these days, getting stem cell samples are difficult in a short time, and such banks with proper legal consent may help the scientific community.

Abstract. Background:. Mastectomy techniques have been extended to nipple-sparing mastectomy (NSM). This study aimed to assess the actual application of NSM in China and identify the factors influencing postoperative complications. Methods:. The clinical data of 615 patients (641 surgeries) undergoing NSM from January 1st, 2018 to December 31st, 2018 at 28 centers nationwide were retrospectively analyzed to obtain the rate of NSM and investigate factors related to NSM surgery. Results:. The proportion of NSM surgery performed in this study was 2.67% (17/641). Malignant breast tumors accounted for the majority of NSM surgery (559/641, 87.2%). A total of 475 (77.3%) patients underwent NSM combined with reconstructive surgery. The rate of reconstruction decreased with age in our study, and implants were the most common option (344/641, 53.7%) in reconstruction. Radial incision was the most selected method regardless of reconstruction. However, for those who underwent reconstruction surgery, 18.4% (85/462) of cases also chose curvilinear incision, while in the simple NSM surgery group, more patients chose circumareolar incision (26/136, 19.1%). The tumor-to-nipple distance (TND) influenced postoperative complications (P = 0.004). There were no relationships between postoperative complications and tumor size, tumor location, histologic grade, molecular subtype, nipple discharge, and axillary lymph nodes. Conclusions:. NSM surgery is feasible and only TND influenced postoperative complications of NSM surgery. But the proportion of NSM surgery performed is still low in nationwide centers of China. The selection criteria for appropriate surgical methods are important for NSM in clinical practice. To optimize clinical applications of NSM, further multicenter prospective randomized controlled studies are needed. Trial Registration:. ChiCTR.org.cn, ChiCTR1900027423; http://www.chictr.org.cn/showprojen.aspx?proj=38739