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2 results on '"Bezdenezhnih NA"'

1. Mechanochemically activated doxorubicin nanoparticles in combination with 40 MHz frequency irradiation on A-549 lung carcinoma cells.

Author

Orel VE, Kudryavets YI, Satz S, Bezdenezhnih NA, Danko ML, Khranovskaya NN, Romanov AV, Dzyatkovskaya NN, and Burlaka AP

Subjects
Algorithms, Antibiotics, Antineoplastic administration & dosage, Antibiotics, Antineoplastic chemistry, Antibiotics, Antineoplastic pharmacology, Apoptosis drug effects, Apoptosis radiation effects, Cell Cycle drug effects, Cell Cycle radiation effects, Cell Line, Tumor, Cell Survival drug effects, Cell Survival radiation effects, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Doxorubicin administration & dosage, Doxorubicin chemistry, Drug Delivery Systems methods, Electromagnetic Fields, Electron Spin Resonance Spectroscopy, Fractals, Free Radicals chemistry, Humans, Lung Neoplasms drug therapy, Lung Neoplasms pathology, Lung Neoplasms radiotherapy, Microscopy, Electron, Scanning, Nanostructures ultrastructure, Particle Size, Powders, Cell Proliferation drug effects, Cell Proliferation radiation effects, Doxorubicin pharmacology, Nanostructures chemistry
Abstract

Targeting of mechanochemically activated doxorubicin (MA DOXO) nanoparticles, conventional doxorubicin, and electromagnetic irradiation (EMI) at A-549 lung carcinoma cells in vitro was investigated. Conventional DOXO was micronized using an input energy of 20 W/g for 5 min resulting in positively charged MA DOXO particles 10 times smaller than conventional DOXO. Mechanochemical activation gives rise to additional free quinone radicals. High performance liquid chromatograph analyses demonstrate that conventional and MA DOXO are quantitatively similar. Tumor cells were exposed to 40 MHz electromagnetic irradiation at a power density of 2 W/cm2. The lethal dose LD50 values of MA DOXO were 5 times greater than conventional doxorubicin. MA DOXO in combination with EMI at 37 degrees C demonstrates improved drug delivery to A-549 human lung carcinoma and greater cell kill than does conventional DOXO.

Published
2005
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2. Effects of mechanochemically activated doxorubicin and 40 MHz frequency irradiation on human A-549 lung carcinoma cells.

Author

Orel VE, Kudryavets YI, Satz S, Bezdenezhnih NA, Danko MI, Khranovskaya NN, Romanov AV, Dzyatkovskaya NN, Burlaka AP, and Sidorik EP

Subjects
Drug Delivery Systems, Friction, Humans, Particle Size, Temperature, Tumor Cells, Cultured, Antibiotics, Antineoplastic pharmacokinetics, Antibiotics, Antineoplastic pharmacology, Carcinoma pathology, Doxorubicin pharmacokinetics, Doxorubicin pharmacology, Electromagnetic Fields, Lung Neoplasms pathology
Abstract

Aim: To study in vitro influence of mechanochemically activated (MA) doxorubicin (DOXO) and electromagnetic irradiation (EMI) on human lung carcinoma A-549 cells., Methods: Solid state DOXO was MA by input energy 20 W/g during 5 min. Tumor cells were exposed to 40 MHz EMI with power density 2 W/cm(2) at temperature 37 degrees C., Results: Particles of MA DOXO have sizes 10 time smaller than officinal DOXO, high performance liquid chromatography analysis showed that parameters of officinal and MA DOXO were quantitatively equal. Mechanochemical activation initiated in the drug formation of free radicals with g = 2.005, g = 2.003 and g = 1.97. LD(50) values of MA DOXO were 5 times lower than that of officinal drug. Cell survival decreased in the following way after effects EMI --> officinal DOXO --> MA DOXO --> officinal DOXO + EMI --> MA DOXO + EMI., Conclusion: Treatment by MA DOXO and drug with EMI at 37 degrees C showed better targeting of drug in human lung carcinoma A-549 cells outcomes than officinal DOXO.

Published
2004

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