1. Damage to the Optic Chiasm in Myelin Oligodendrocyte Glycoprotein–Experimental Autoimmune Encephalomyelitis Mice
- Author
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Angela E. Schellenberg, Vanessa L. Palmer, Heather T. Whittaker, Jonathan D. Thiessen, Marc R. Del Bigio, Annie Kim, Richard Buist, Blair Cardigan Smith, Sheryl L. Herrera, and Melanie Martin
- Subjects
lcsh:Medical physics. Medical radiology. Nuclear medicine ,Pathology ,medicine.medical_specialty ,lcsh:R895-920 ,experimental autoimmune encephalomyelitis ,H&E stain ,Optic chiasm ,Myelin oligodendrocyte glycoprotein ,03 medical and health sciences ,Myelin ,0302 clinical medicine ,medicine ,magnetic resonance imaging ,Optic neuritis ,mouse ,Original Research ,030304 developmental biology ,0303 health sciences ,pertussis toxin ,biology ,medicine.diagnostic_test ,business.industry ,optic chiasm ,Experimental autoimmune encephalomyelitis ,Magnetic resonance imaging ,medicine.disease ,Hyperintensity ,medicine.anatomical_structure ,biology.protein ,business ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Optic chiasm lesions in myelin oligodendrocyte glycoprotein (MOG)–experimental autoimmune encephalomyelitis (EAE) mice were characterized using magnetic resonance imaging (MRI) and validated using electron microscopy (EM). MR images were collected from 3 days after induction to remission, approximately 20 days after induction. Hematoxylin and eosin, solochrome cyanin–stained sections, and EM images were obtained from the optic chiasms of some mice approximately 4 days after disease onset when their scores were thought to be the highest. T2-weighted imaging and apparent diffusion coefficient map hyperintensities corresponded to abnormalities in the optic chiasms of EAE mice. Mixed inflammation was concentrated at the lateral surface. Degeneration of oligodendrocytes, myelin, and early axonal damage were also apparent. A marked increase in chiasm thickness was observed. T2-weighted and diffusion-weighted MRI can detect abnormalities in the optic chiasms of MOG-EAE mice. MRI is an important method in the study of this model toward understanding optic neuritis.
- Published
- 2014